LOCUS       DACDRK010000001       129106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDRK010000001 DACDRK010000000
VERSION     DACDRK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 129106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 129106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..129106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.001"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(12..1535)
                     /locus_tag="IWJ77_000001"
     CDS             complement(12..1535)
                     /locus_tag="IWJ77_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9451655.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="IWJ77_000002"
     CDS             1787..2968
                     /locus_tag="IWJ77_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9451656.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IWJ77_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IWJ77_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9451657.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="IWJ77_000004"
     CDS             complement(5053..7764)
                     /locus_tag="IWJ77_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9451658.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="IWJ77_000005"
     CDS             complement(7774..8475)
                     /locus_tag="IWJ77_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9451659.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="IWJ77_000006"
     CDS             8661..9260
                     /locus_tag="IWJ77_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9451660.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="IWJ77_000007"
     CDS             9341..10354
                     /locus_tag="IWJ77_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9451661.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="IWJ77_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="IWJ77_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9451662.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="IWJ77_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="IWJ77_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9451663.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="IWJ77_000010"
     CDS             11802..13175
                     /locus_tag="IWJ77_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9451664.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="IWJ77_000011"
     CDS             13532..15676
                     /locus_tag="IWJ77_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9451665.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="IWJ77_000012"
     CDS             complement(15728..16768)
                     /locus_tag="IWJ77_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9451666.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="IWJ77_000013"
     CDS             complement(17045..18847)
                     /locus_tag="IWJ77_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9451667.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="IWJ77_000014"
     CDS             complement(18861..19613)
                     /locus_tag="IWJ77_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9451668.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="IWJ77_000015"
     CDS             complement(19622..19984)
                     /locus_tag="IWJ77_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9451669.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="IWJ77_000016"
     CDS             complement(20160..21032)
                     /locus_tag="IWJ77_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9451670.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="IWJ77_000017"
     CDS             complement(21058..21570)
                     /locus_tag="IWJ77_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9451671.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22538)
                     /locus_tag="IWJ77_000018"
     CDS             complement(21708..22538)
                     /locus_tag="IWJ77_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9451672.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(22554..23162)
                     /locus_tag="IWJ77_000019"
     CDS             complement(22554..23162)
                     /locus_tag="IWJ77_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9451673.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="IWJ77_000020"
     CDS             complement(23155..23472)
                     /locus_tag="IWJ77_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9451674.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="IWJ77_000021"
     CDS             complement(23628..24356)
                     /locus_tag="IWJ77_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9451675.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="IWJ77_000022"
     CDS             24515..25396
                     /locus_tag="IWJ77_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9451676.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="IWJ77_000023"
     CDS             complement(25633..26676)
                     /locus_tag="IWJ77_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9451677.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="IWJ77_000024"
     CDS             complement(26679..27347)
                     /locus_tag="IWJ77_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9451678.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="IWJ77_000025"
     CDS             27489..29825
                     /locus_tag="IWJ77_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9451679.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="IWJ77_000026"
     CDS             complement(29940..30251)
                     /locus_tag="IWJ77_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451680.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="IWJ77_000027"
     CDS             30432..31907
                     /locus_tag="IWJ77_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9451681.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="IWJ77_000028"
     CDS             31954..32538
                     /locus_tag="IWJ77_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9451682.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="IWJ77_000029"
     CDS             32540..32959
                     /locus_tag="IWJ77_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9451683.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="IWJ77_000030"
     CDS             complement(33240..33524)
                     /locus_tag="IWJ77_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9451684.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="IWJ77_000031"
     CDS             complement(33876..34586)
                     /locus_tag="IWJ77_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9451685.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="IWJ77_000032"
     CDS             complement(34583..36055)
                     /locus_tag="IWJ77_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9451686.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="IWJ77_000033"
     CDS             36193..36666
                     /locus_tag="IWJ77_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9451687.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="IWJ77_000034"
     CDS             complement(36819..37370)
                     /locus_tag="IWJ77_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9451688.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="IWJ77_000035"
     CDS             37475..38005
                     /locus_tag="IWJ77_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9451689.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="IWJ77_000036"
     CDS             complement(38488..38931)
                     /locus_tag="IWJ77_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9451690.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="IWJ77_000037"
     CDS             complement(38947..40107)
                     /locus_tag="IWJ77_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9451691.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="IWJ77_000038"
     CDS             40614..42314
                     /locus_tag="IWJ77_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451692.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="IWJ77_000039"
     CDS             complement(42416..43249)
                     /locus_tag="IWJ77_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9451693.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="IWJ77_000040"
     CDS             complement(43553..45718)
                     /locus_tag="IWJ77_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9451694.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="IWJ77_000041"
     CDS             45978..48272
                     /locus_tag="IWJ77_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9451695.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="IWJ77_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="IWJ77_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9451696.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="IWJ77_000043"
     CDS             48923..49930
                     /locus_tag="IWJ77_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9451697.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="IWJ77_000044"
     CDS             49952..50971
                     /locus_tag="IWJ77_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9451698.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="IWJ77_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="IWJ77_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9451699.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="IWJ77_000046"
     CDS             complement(52729..54150)
                     /locus_tag="IWJ77_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9451700.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="IWJ77_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="IWJ77_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9451701.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="IWJ77_000048"
     CDS             complement(56561..57817)
                     /locus_tag="IWJ77_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9451702.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            58397..59698
                     /locus_tag="IWJ77_000049"
     CDS             58397..59698
                     /locus_tag="IWJ77_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9451703.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="IWJ77_000050"
     CDS             complement(59767..60366)
                     /locus_tag="IWJ77_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9451704.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="IWJ77_000051"
     CDS             60620..61501
                     /locus_tag="IWJ77_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9451705.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="IWJ77_000052"
     CDS             61514..63124
                     /locus_tag="IWJ77_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9451706.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="IWJ77_000053"
     CDS             complement(63201..63860)
                     /locus_tag="IWJ77_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9451707.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="IWJ77_000054"
     CDS             complement(64143..64808)
                     /locus_tag="IWJ77_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451708.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="IWJ77_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="IWJ77_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9451709.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66571)
                     /gene="dhaK"
                     /locus_tag="IWJ77_000056"
     CDS             complement(65588..66571)
                     /gene="dhaK"
                     /locus_tag="IWJ77_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9451710.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="IWJ77_000057"
     CDS             complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="IWJ77_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9451711.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="IWJ77_000058"
     CDS             67122..67442
                     /locus_tag="IWJ77_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9451712.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="IWJ77_000059"
     CDS             complement(67554..68423)
                     /locus_tag="IWJ77_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9451713.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69793)
                     /locus_tag="IWJ77_000060"
     CDS             complement(68519..69793)
                     /locus_tag="IWJ77_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9451714.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(69819..70328)
                     /locus_tag="IWJ77_000061"
     CDS             complement(69819..70328)
                     /locus_tag="IWJ77_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451715.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="IWJ77_000062"
     CDS             complement(70503..72638)
                     /locus_tag="IWJ77_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9451716.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="IWJ77_000063"
     CDS             complement(72721..74376)
                     /locus_tag="IWJ77_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9451717.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="IWJ77_000064"
     CDS             complement(74378..74692)
                     /locus_tag="IWJ77_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9451718.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="IWJ77_000065"
     CDS             74862..75122
                     /locus_tag="IWJ77_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9451719.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="IWJ77_000066"
     CDS             75136..75450
                     /locus_tag="IWJ77_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9451720.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="IWJ77_000067"
     CDS             complement(76180..76734)
                     /locus_tag="IWJ77_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9451721.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="IWJ77_000068"
     CDS             complement(76860..78788)
                     /locus_tag="IWJ77_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9451722.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="IWJ77_000069"
     CDS             complement(79094..79810)
                     /locus_tag="IWJ77_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9451723.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="IWJ77_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="IWJ77_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9451724.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="IWJ77_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="IWJ77_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9451725.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="IWJ77_000072"
     CDS             complement(83280..84740)
                     /locus_tag="IWJ77_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9451726.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="IWJ77_000073"
     CDS             complement(84730..86025)
                     /locus_tag="IWJ77_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9451727.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="IWJ77_000074"
     CDS             complement(86357..87034)
                     /locus_tag="IWJ77_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9451728.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="IWJ77_000075"
     CDS             complement(87064..88155)
                     /locus_tag="IWJ77_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9451729.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="IWJ77_000076"
     CDS             complement(88256..88690)
                     /locus_tag="IWJ77_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9451730.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="IWJ77_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="IWJ77_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9451731.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="IWJ77_000078"
     CDS             complement(90728..92623)
                     /locus_tag="IWJ77_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9451732.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="IWJ77_000079"
     CDS             complement(92681..94456)
                     /locus_tag="IWJ77_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9451733.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="IWJ77_000080"
     CDS             complement(94506..96002)
                     /locus_tag="IWJ77_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9451734.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="IWJ77_000081"
     CDS             complement(96196..97818)
                     /locus_tag="IWJ77_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9451735.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="IWJ77_000082"
     CDS             complement(98267..99580)
                     /locus_tag="IWJ77_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9451736.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="IWJ77_000083"
     CDS             complement(99825..100049)
                     /locus_tag="IWJ77_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451737.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100437)
                     /locus_tag="IWJ77_000084"
     CDS             complement(100069..100437)
                     /locus_tag="IWJ77_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9451738.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="IWJ77_000085"
     CDS             complement(100635..101345)
                     /locus_tag="IWJ77_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451739.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="IWJ77_000086"
     CDS             complement(101363..101860)
                     /locus_tag="IWJ77_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9451740.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="IWJ77_000087"
     CDS             complement(101897..102349)
                     /locus_tag="IWJ77_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9451741.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="IWJ77_000088"
     CDS             complement(102484..103089)
                     /locus_tag="IWJ77_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9451742.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="IWJ77_000089"
     CDS             complement(103539..104159)
                     /locus_tag="IWJ77_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9451743.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="IWJ77_000090"
     CDS             complement(104161..105030)
                     /locus_tag="IWJ77_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9451744.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="IWJ77_000091"
     CDS             complement(105053..105460)
                     /locus_tag="IWJ77_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9451745.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="IWJ77_000092"
     CDS             complement(105479..106768)
                     /locus_tag="IWJ77_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9451746.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="IWJ77_000093"
     CDS             complement(106795..107097)
                     /locus_tag="IWJ77_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9451747.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="IWJ77_000094"
     CDS             complement(107122..109215)
                     /locus_tag="IWJ77_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9451748.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="IWJ77_000095"
     CDS             complement(109704..110468)
                     /locus_tag="IWJ77_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9451749.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="IWJ77_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="IWJ77_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9451750.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="IWJ77_000097"
     CDS             complement(111473..112549)
                     /locus_tag="IWJ77_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9451751.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="IWJ77_000098"
     CDS             complement(112573..113166)
                     /locus_tag="IWJ77_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9451752.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="IWJ77_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="IWJ77_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9451753.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="IWJ77_000100"
     CDS             complement(114836..115135)
                     /locus_tag="IWJ77_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9451754.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="IWJ77_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="IWJ77_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9451755.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="IWJ77_000102"
     CDS             complement(116890..117801)
                     /locus_tag="IWJ77_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9451756.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="IWJ77_000103"
     CDS             117921..118778
                     /locus_tag="IWJ77_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9451757.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="IWJ77_000104"
     CDS             complement(118965..119327)
                     /locus_tag="IWJ77_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451758.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="IWJ77_000105"
     CDS             119673..121112
                     /locus_tag="IWJ77_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9451759.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="IWJ77_000106"
     CDS             complement(121119..123671)
                     /locus_tag="IWJ77_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9451760.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="IWJ77_000107"
     CDS             complement(123785..125116)
                     /locus_tag="IWJ77_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9451761.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="IWJ77_000108"
     CDS             complement(125082..126560)
                     /locus_tag="IWJ77_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9451762.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="IWJ77_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="IWJ77_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9451763.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        37793 a        26983 c        22557 g        41773 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggac ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
   128941 ataaaatgac tgtccttcat tcactaaaca tatttttatt tttttatatt gttaaaaatt
   129001 cacaatttat tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt
   129061 tttaacggca tttatatgct ttttcagaaa ttaactgatg atttca
//
LOCUS       DACDRK010000002       127816 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDRK010000002 DACDRK010000000
VERSION     DACDRK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 127816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 127816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..127816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.002"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWJ77_000110"
     CDS             212..604
                     /locus_tag="IWJ77_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9451764.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWJ77_000111"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWJ77_000111"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9451765.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWJ77_000112"
     CDS             3165..4775
                     /locus_tag="IWJ77_000112"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9451766.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWJ77_000113"
     CDS             5067..5936
                     /locus_tag="IWJ77_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9451767.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWJ77_000114"
     CDS             6101..7372
                     /locus_tag="IWJ77_000114"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9451768.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWJ77_000115"
     CDS             7369..8664
                     /locus_tag="IWJ77_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9451769.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWJ77_000116"
     CDS             8763..9026
                     /locus_tag="IWJ77_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9451770.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWJ77_000117"
     CDS             9471..9920
                     /locus_tag="IWJ77_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9451771.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWJ77_000118"
     CDS             9977..11395
                     /locus_tag="IWJ77_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9451772.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWJ77_000119"
     CDS             11395..12255
                     /locus_tag="IWJ77_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9451773.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWJ77_000120"
     CDS             12429..13202
                     /locus_tag="IWJ77_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9451774.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWJ77_000121"
     CDS             13349..13714
                     /locus_tag="IWJ77_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9451775.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWJ77_000122"
     CDS             complement(13862..15070)
                     /locus_tag="IWJ77_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9451776.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWJ77_000123"
     CDS             complement(15089..15367)
                     /locus_tag="IWJ77_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451777.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWJ77_000124"
     CDS             15560..16216
                     /locus_tag="IWJ77_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9451778.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWJ77_000125"
     CDS             complement(16658..17455)
                     /locus_tag="IWJ77_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9451779.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWJ77_000126"
     CDS             complement(17452..18636)
                     /locus_tag="IWJ77_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9451780.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWJ77_000127"
     CDS             complement(18661..19908)
                     /locus_tag="IWJ77_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9451781.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWJ77_000128"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWJ77_000128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9451782.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWJ77_000129"
     CDS             21888..22202
                     /locus_tag="IWJ77_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9451783.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWJ77_000130"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWJ77_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9451784.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWJ77_000131"
     CDS             22887..23531
                     /locus_tag="IWJ77_000131"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9451785.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWJ77_000132"
     CDS             23546..23875
                     /locus_tag="IWJ77_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451786.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWJ77_000133"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWJ77_000133"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9451787.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWJ77_000134"
     CDS             24863..25687
                     /locus_tag="IWJ77_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9451788.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWJ77_000135"
     CDS             25680..26027
                     /locus_tag="IWJ77_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9451789.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWJ77_000136"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWJ77_000136"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9451790.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWJ77_000137"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWJ77_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9451791.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWJ77_000138"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWJ77_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9451792.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWJ77_000139"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWJ77_000139"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9451793.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWJ77_000140"
     CDS             31684..32451
                     /locus_tag="IWJ77_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9451794.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWJ77_000141"
     CDS             32441..34483
                     /locus_tag="IWJ77_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9451795.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IWJ77_000142"
     CDS             34764..34913
                     /locus_tag="IWJ77_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9451796.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IWJ77_000143"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IWJ77_000143"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9451797.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IWJ77_000144"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IWJ77_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9451798.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IWJ77_000145"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IWJ77_000145"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9451799.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IWJ77_000146"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IWJ77_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9451800.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IWJ77_000147"
     CDS             39330..40412
                     /locus_tag="IWJ77_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9451801.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IWJ77_000148"
     CDS             40412..41215
                     /locus_tag="IWJ77_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9451802.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IWJ77_000149"
     CDS             41285..42025
                     /locus_tag="IWJ77_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9451803.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IWJ77_000150"
     CDS             42036..43040
                     /locus_tag="IWJ77_000150"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9451804.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="IWJ77_000151"
     CDS             complement(43085..44929)
                     /locus_tag="IWJ77_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451805.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="IWJ77_000152"
     CDS             45141..45659
                     /locus_tag="IWJ77_000152"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9451806.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="IWJ77_000153"
     CDS             45787..46470
                     /locus_tag="IWJ77_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9451807.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="IWJ77_000154"
     tRNA            46779..46852
                     /locus_tag="IWJ77_000154"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="IWJ77_000155"
     CDS             47011..47370
                     /locus_tag="IWJ77_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9451808.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="IWJ77_000156"
     CDS             47493..49124
                     /locus_tag="IWJ77_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9451809.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="IWJ77_000157"
     CDS             complement(49186..49827)
                     /locus_tag="IWJ77_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9451810.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="IWJ77_000158"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="IWJ77_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9451811.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="IWJ77_000159"
     CDS             50444..50788
                     /locus_tag="IWJ77_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9451812.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="IWJ77_000160"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="IWJ77_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9451813.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51501..54335
                     /locus_tag="IWJ77_000161"
     CDS             51501..54335
                     /locus_tag="IWJ77_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9451814.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="IWJ77_000162"
     CDS             54526..55017
                     /locus_tag="IWJ77_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9451815.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="IWJ77_000163"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="IWJ77_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9451816.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="IWJ77_000164"
     CDS             56142..56945
                     /locus_tag="IWJ77_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9451817.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="IWJ77_000165"
     CDS             56966..57880
                     /locus_tag="IWJ77_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9451818.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="IWJ77_000166"
     CDS             57963..58337
                     /locus_tag="IWJ77_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9451819.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="IWJ77_000167"
     CDS             58476..58721
                     /locus_tag="IWJ77_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9451820.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="IWJ77_000168"
     CDS             58893..59957
                     /locus_tag="IWJ77_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9451821.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="IWJ77_000169"
     CDS             60203..60628
                     /locus_tag="IWJ77_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9451822.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..61070
                     /gene="nusB"
                     /locus_tag="IWJ77_000170"
     CDS             60621..61070
                     /gene="nusB"
                     /locus_tag="IWJ77_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9451823.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61167..62021
                     /locus_tag="IWJ77_000171"
     CDS             61167..62021
                     /locus_tag="IWJ77_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9451824.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62022..63359
                     /locus_tag="IWJ77_000172"
     CDS             62022..63359
                     /locus_tag="IWJ77_000172"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9451825.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63364..63588
                     /locus_tag="IWJ77_000173"
     CDS             63364..63588
                     /locus_tag="IWJ77_000173"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9451826.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63590..64480
                     /locus_tag="IWJ77_000174"
     CDS             63590..64480
                     /locus_tag="IWJ77_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9451827.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64489..65307
                     /locus_tag="IWJ77_000175"
     CDS             64489..65307
                     /locus_tag="IWJ77_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9451828.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65414..65863
                     /locus_tag="IWJ77_000176"
     CDS             65414..65863
                     /locus_tag="IWJ77_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9451829.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65881..67563
                     /gene="recN"
                     /locus_tag="IWJ77_000177"
     CDS             65881..67563
                     /gene="recN"
                     /locus_tag="IWJ77_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9451830.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67618..67734)
                     /locus_tag="IWJ77_000178"
     CDS             complement(67618..67734)
                     /locus_tag="IWJ77_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9451831.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67807..68763)
                     /locus_tag="IWJ77_000179"
     CDS             complement(67807..68763)
                     /locus_tag="IWJ77_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9451832.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68934..69302
                     /locus_tag="IWJ77_000180"
     CDS             68934..69302
                     /locus_tag="IWJ77_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9451833.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69472..69903
                     /gene="mraZ"
                     /locus_tag="IWJ77_000181"
     CDS             69472..69903
                     /gene="mraZ"
                     /locus_tag="IWJ77_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9451834.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69924..70883
                     /gene="rsmH"
                     /locus_tag="IWJ77_000182"
     CDS             69924..70883
                     /gene="rsmH"
                     /locus_tag="IWJ77_000182"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9451835.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70888..71289
                     /gene="ftsL"
                     /locus_tag="IWJ77_000183"
     CDS             70888..71289
                     /gene="ftsL"
                     /locus_tag="IWJ77_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9451836.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71290..73482
                     /locus_tag="IWJ77_000184"
     CDS             71290..73482
                     /locus_tag="IWJ77_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9451837.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73508..74470
                     /locus_tag="IWJ77_000185"
     CDS             73508..74470
                     /locus_tag="IWJ77_000185"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9451838.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74484..75845
                     /locus_tag="IWJ77_000186"
     CDS             74484..75845
                     /locus_tag="IWJ77_000186"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9451839.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75871..76959
                     /gene="murG"
                     /locus_tag="IWJ77_000187"
     CDS             75871..76959
                     /gene="murG"
                     /locus_tag="IWJ77_000187"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9451840.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76974..78194
                     /locus_tag="IWJ77_000188"
     CDS             76974..78194
                     /locus_tag="IWJ77_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9451841.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78350..79675
                     /gene="ftsA"
                     /locus_tag="IWJ77_000189"
     CDS             78350..79675
                     /gene="ftsA"
                     /locus_tag="IWJ77_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9451842.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79698..80939
                     /gene="ftsZ"
                     /locus_tag="IWJ77_000190"
     CDS             79698..80939
                     /gene="ftsZ"
                     /locus_tag="IWJ77_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9451843.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80956..81633
                     /locus_tag="IWJ77_000191"
     CDS             80956..81633
                     /locus_tag="IWJ77_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9451844.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81647..82246
                     /locus_tag="IWJ77_000192"
     CDS             81647..82246
                     /locus_tag="IWJ77_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9451845.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82261..82548
                     /locus_tag="IWJ77_000193"
     CDS             82261..82548
                     /locus_tag="IWJ77_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9451846.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82597..83379
                     /locus_tag="IWJ77_000194"
     CDS             82597..83379
                     /locus_tag="IWJ77_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9451847.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83400..84107
                     /locus_tag="IWJ77_000195"
     CDS             83400..84107
                     /locus_tag="IWJ77_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9451848.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84369..87167
                     /gene="ileS"
                     /locus_tag="IWJ77_000196"
     CDS             84369..87167
                     /gene="ileS"
                     /locus_tag="IWJ77_000196"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9451849.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87216..88742)
                     /locus_tag="IWJ77_000197"
     CDS             complement(87216..88742)
                     /locus_tag="IWJ77_000197"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9451850.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88757..89404)
                     /locus_tag="IWJ77_000198"
     CDS             complement(88757..89404)
                     /locus_tag="IWJ77_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9451851.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89588..89917)
                     /locus_tag="IWJ77_000199"
     CDS             complement(89588..89917)
                     /locus_tag="IWJ77_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9451852.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90094..90822
                     /gene="sfsA"
                     /locus_tag="IWJ77_000200"
     CDS             90094..90822
                     /gene="sfsA"
                     /locus_tag="IWJ77_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9451853.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90838..91851
                     /locus_tag="IWJ77_000201"
     CDS             90838..91851
                     /locus_tag="IWJ77_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9451854.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91851..93128
                     /locus_tag="IWJ77_000202"
     CDS             91851..93128
                     /locus_tag="IWJ77_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9451855.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93191..95893
                     /locus_tag="IWJ77_000203"
     CDS             93191..95893
                     /locus_tag="IWJ77_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9451856.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96045..96362
                     /locus_tag="IWJ77_000204"
     CDS             96045..96362
                     /locus_tag="IWJ77_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9451857.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96392..96712
                     /locus_tag="IWJ77_000205"
     CDS             96392..96712
                     /locus_tag="IWJ77_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9451858.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96820..98280
                     /locus_tag="IWJ77_000206"
     CDS             96820..98280
                     /locus_tag="IWJ77_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9451859.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98348..98569
                     /locus_tag="IWJ77_000207"
     CDS             98348..98569
                     /locus_tag="IWJ77_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451860.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98600..98782
                     /locus_tag="IWJ77_000208"
     CDS             98600..98782
                     /locus_tag="IWJ77_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9451861.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98782..99195
                     /locus_tag="IWJ77_000209"
     CDS             98782..99195
                     /locus_tag="IWJ77_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9451862.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99318..100499
                     /locus_tag="IWJ77_000210"
     CDS             99318..100499
                     /locus_tag="IWJ77_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9451863.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            100888..100961
                     /locus_tag="IWJ77_000211"
     tRNA            100888..100961
                     /locus_tag="IWJ77_000211"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100922..100924,aa:Arg,seq:cct)
     gene            complement(100998..101198)
                     /locus_tag="IWJ77_000212"
     CDS             complement(100998..101198)
                     /locus_tag="IWJ77_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9451864.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(101503..102735)
                     /locus_tag="IWJ77_000213"
     CDS             complement(101503..102735)
                     /locus_tag="IWJ77_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9451865.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            102989..103558
                     /locus_tag="IWJ77_000214"
     CDS             102989..103558
                     /locus_tag="IWJ77_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9451866.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(103736..104176)
                     /locus_tag="IWJ77_000215"
     CDS             complement(103736..104176)
                     /locus_tag="IWJ77_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9451867.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            104334..105098
                     /gene="map"
                     /locus_tag="IWJ77_000216"
     CDS             104334..105098
                     /gene="map"
                     /locus_tag="IWJ77_000216"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9451868.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            105130..106053
                     /locus_tag="IWJ77_000217"
     CDS             105130..106053
                     /locus_tag="IWJ77_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9451869.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            106129..107268
                     /locus_tag="IWJ77_000218"
     CDS             106129..107268
                     /locus_tag="IWJ77_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9451870.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            107261..108061
                     /locus_tag="IWJ77_000219"
     CDS             107261..108061
                     /locus_tag="IWJ77_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9451871.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            108061..108888
                     /locus_tag="IWJ77_000220"
     CDS             108061..108888
                     /locus_tag="IWJ77_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9451872.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            108866..109600
                     /locus_tag="IWJ77_000221"
     CDS             108866..109600
                     /locus_tag="IWJ77_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9451873.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            109700..110566
                     /gene="rfbA"
                     /locus_tag="IWJ77_000222"
     CDS             109700..110566
                     /gene="rfbA"
                     /locus_tag="IWJ77_000222"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9451874.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            110580..111152
                     /gene="rfbC"
                     /locus_tag="IWJ77_000223"
     CDS             110580..111152
                     /gene="rfbC"
                     /locus_tag="IWJ77_000223"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9451875.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            111174..112202
                     /gene="rfbB"
                     /locus_tag="IWJ77_000224"
     CDS             111174..112202
                     /gene="rfbB"
                     /locus_tag="IWJ77_000224"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9451876.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            112300..113151
                     /gene="rfbD"
                     /locus_tag="IWJ77_000225"
     CDS             112300..113151
                     /gene="rfbD"
                     /locus_tag="IWJ77_000225"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9451877.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            113186..115219
                     /locus_tag="IWJ77_000226"
     CDS             113186..115219
                     /locus_tag="IWJ77_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451878.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            115263..116543
                     /locus_tag="IWJ77_000227"
     CDS             115263..116543
                     /locus_tag="IWJ77_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9451879.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            116753..117559
                     /locus_tag="IWJ77_000228"
     CDS             116753..117559
                     /locus_tag="IWJ77_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9451880.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            117571..118791
                     /locus_tag="IWJ77_000229"
     CDS             117571..118791
                     /locus_tag="IWJ77_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9451881.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            118781..120367
                     /locus_tag="IWJ77_000230"
     CDS             118781..120367
                     /locus_tag="IWJ77_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9451882.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            120406..122544
                     /locus_tag="IWJ77_000231"
     CDS             120406..122544
                     /locus_tag="IWJ77_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9451883.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(122912..123895)
                     /locus_tag="IWJ77_000232"
     CDS             complement(122912..123895)
                     /locus_tag="IWJ77_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9451884.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            124273..125664
                     /locus_tag="IWJ77_000233"
     CDS             124273..125664
                     /locus_tag="IWJ77_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9451885.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            125680..126720
                     /locus_tag="IWJ77_000234"
     CDS             125680..126720
                     /locus_tag="IWJ77_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9451886.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            126739..>127816
                     /locus_tag="IWJ77_000235"
     CDS             126739..>127816
                     /locus_tag="IWJ77_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9451887.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSR"
BASE COUNT        41572 a        21779 c        26887 g        37578 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    61081 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    61141 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    61201 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    61261 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    61321 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    61381 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    61441 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    61501 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    61561 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    61621 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    61681 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    61741 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    61801 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    61861 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    61921 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    61981 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    62041 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    62101 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    62161 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    62221 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    62281 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    62341 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    62401 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    62461 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    62521 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    62581 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    62641 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    62701 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    62761 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    62821 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    62881 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    62941 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    63001 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    63061 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    63121 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    63181 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    63241 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    63301 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    63361 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    63421 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    63481 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    63541 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    63601 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    63661 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    63721 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    63781 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    63841 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    63901 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    63961 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    64021 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    64081 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    64141 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    64201 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    64261 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    64321 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    64381 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    64441 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    64501 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    64561 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    64621 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    64681 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    64741 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    64801 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    64861 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    64921 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    64981 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    65041 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    65101 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    65161 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    65221 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    65281 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    65341 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    65401 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    65461 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    65521 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    65581 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    65641 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    65701 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    65761 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    65821 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    65881 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    65941 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    66001 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    66061 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    66121 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    66181 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    66241 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    66301 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    66361 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    66421 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    66481 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    66541 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    66601 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    66661 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    66721 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    66781 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    66841 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    66901 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    66961 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    67021 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    67081 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    67141 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    67201 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    67261 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    67321 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    67381 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    67441 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    67501 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    67561 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    67621 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    67681 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    67741 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    67801 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    67861 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    67921 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    67981 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    68041 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    68101 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    68161 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    68221 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    68281 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    68341 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    68401 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    68461 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    68521 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    68581 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    68641 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    68701 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    68761 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    68821 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    68881 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    68941 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    69001 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    69061 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    69121 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    69181 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    69241 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    69301 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    69361 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    69421 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    69481 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    69541 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    69601 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    69661 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    69721 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    69781 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    69841 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    69901 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    69961 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    70021 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    70081 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    70141 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    70201 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    70261 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    70321 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    70381 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    70441 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    70501 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    70561 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    70621 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    70681 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    70741 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    70801 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    70861 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    70921 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    70981 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    71041 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    71101 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    71161 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    71221 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    71281 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    71341 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    71401 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    71461 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    71521 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    71581 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    71641 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    71701 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    71761 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    71821 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    71881 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    71941 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    72001 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    72061 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    72121 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    72181 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    72241 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    72301 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    72361 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    72421 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    72481 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    72541 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    72601 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    72661 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    72721 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    72781 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    72841 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaaac
    72901 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    72961 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    73021 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    73081 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    73141 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    73201 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    73261 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    73321 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    73381 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    73441 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    73501 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    73561 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    73621 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    73681 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    73741 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    73801 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    73861 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    73921 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    73981 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    74041 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    74101 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    74161 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    74221 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    74281 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    74341 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    74401 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    74461 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    74521 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    74581 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    74641 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    74701 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    74761 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    74821 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    74881 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    74941 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    75001 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    75061 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    75121 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    75181 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    75241 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    75301 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    75361 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    75421 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    75481 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    75541 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    75601 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    75661 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    75721 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    75781 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    75841 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    75901 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    75961 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    76021 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    76081 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    76141 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    76201 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    76261 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    76321 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    76381 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    76441 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    76501 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    76561 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    76621 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    76681 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    76741 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    76801 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    76861 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    76921 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    76981 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    77041 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    77101 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    77161 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    77221 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    77281 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    77341 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    77401 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    77461 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    77521 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    77581 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    77641 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    77701 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    77761 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    77821 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    77881 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    77941 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    78001 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    78061 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    78121 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    78181 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    78241 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    78301 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    78361 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    78421 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    78481 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    78541 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    78601 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    78661 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    78721 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    78781 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    78841 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    78901 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    78961 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    79021 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    79081 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    79141 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    79201 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    79261 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    79321 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    79381 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    79441 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    79501 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    79561 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    79621 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    79681 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    79741 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    79801 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    79861 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    79921 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    79981 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    80041 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    80101 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    80161 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    80221 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    80281 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    80341 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    80401 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    80461 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    80521 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    80581 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    80641 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    80701 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    80761 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    80821 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    80881 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    80941 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    81001 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    81061 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    81121 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    81181 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    81241 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    81301 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    81361 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    81421 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    81481 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    81541 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    81601 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    81661 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    81721 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    81781 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    81841 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    81901 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    81961 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    82021 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    82081 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    82141 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    82201 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    82261 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    82321 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    82381 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    82441 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    82501 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    82561 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    82621 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    82681 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    82741 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    82801 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    82861 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    82921 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    82981 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    83041 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    83101 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    83161 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    83221 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    83281 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    83341 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    83401 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    83461 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    83521 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    83581 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    83641 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    83701 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    83761 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    83821 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    83881 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    83941 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    84001 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    84061 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    84121 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    84181 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    84241 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    84301 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    84361 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    84421 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    84481 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    84541 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    84601 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    84661 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    84721 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    84781 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    84841 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    84901 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    84961 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    85021 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    85081 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    85141 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    85201 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    85261 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    85321 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    85381 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    85441 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    85501 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    85561 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    85621 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    85681 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    85741 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    85801 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    85861 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    85921 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    85981 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    86041 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    86101 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    86161 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    86221 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    86281 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    86341 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    86401 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    86461 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    86521 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    86581 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    86641 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    86701 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    86761 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    86821 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    86881 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    86941 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    87001 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    87061 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    87121 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    87181 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    87241 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    87301 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    87361 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    87421 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    87481 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    87541 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    87601 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    87661 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    87721 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    87781 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    87841 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    87901 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    87961 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    88021 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    88081 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    88141 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    88201 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    88261 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    88321 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    88381 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    88441 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    88501 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    88561 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    88621 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    88681 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    88741 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    88801 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    88861 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    88921 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    88981 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    89041 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    89101 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    89161 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    89221 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    89281 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    89341 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    89401 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    89461 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    89521 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    89581 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    89641 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    89701 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    89761 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    89821 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    89881 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    89941 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    90001 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    90061 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    90121 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    90181 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    90241 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    90301 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    90361 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    90421 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    90481 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    90541 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    90601 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    90661 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    90721 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    90781 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    90841 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    90901 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    90961 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    91021 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    91081 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    91141 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    91201 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    91261 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    91321 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    91381 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    91441 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    91501 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    91561 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    91621 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    91681 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    91741 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    91801 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    91861 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    91921 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    91981 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    92041 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    92101 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    92161 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    92221 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    92281 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    92341 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    92401 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    92461 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    92521 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    92581 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    92641 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    92701 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    92761 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    92821 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    92881 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    92941 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    93001 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    93061 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    93121 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    93181 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    93241 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    93301 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    93361 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    93421 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    93481 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    93541 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    93601 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    93661 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    93721 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    93781 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    93841 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    93901 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    93961 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    94021 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    94081 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    94141 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    94201 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    94261 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    94321 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    94381 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    94441 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    94501 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    94561 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    94621 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    94681 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    94741 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    94801 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    94861 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    94921 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    94981 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    95041 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    95101 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    95161 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    95221 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    95281 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    95341 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    95401 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    95461 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    95521 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    95581 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    95641 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    95701 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    95761 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    95821 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    95881 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    95941 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    96001 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    96061 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    96121 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    96181 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    96241 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    96301 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    96361 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    96421 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    96481 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    96541 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    96601 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    96661 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    96721 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    96781 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    96841 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    96901 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    96961 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    97021 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    97081 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    97141 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    97201 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    97261 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    97321 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    97381 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    97441 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    97501 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    97561 gggcaactga tcattgattt ggtgcaaaaa tatattgcag agccattcct aggattatct
    97621 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    97681 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    97741 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    97801 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    97861 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    97921 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    97981 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    98041 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    98101 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    98161 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    98221 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    98281 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    98341 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    98401 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    98461 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    98521 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    98581 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    98641 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    98701 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    98761 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    98821 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    98881 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    98941 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    99001 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    99061 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    99121 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    99181 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    99241 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    99301 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    99361 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    99421 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    99481 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    99541 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    99601 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    99661 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    99721 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    99781 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    99841 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    99901 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    99961 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
   100021 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
   100081 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
   100141 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
   100201 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
   100261 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
   100321 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
   100381 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
   100441 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
   100501 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
   100561 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
   100621 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
   100681 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
   100741 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
   100801 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
   100861 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
   100921 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtacaaataa cccctgtatc
   100981 ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa actgctatgt
   101041 cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgc
   101101 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
   101161 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
   101221 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
   101281 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
   101341 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
   101401 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
   101461 tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt
   101521 tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga
   101581 tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc
   101641 atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg
   101701 attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg
   101761 tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag
   101821 cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt
   101881 aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga
   101941 aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt
   102001 tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt
   102061 aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag aaaattgttc
   102121 tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac
   102181 agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg
   102241 tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac
   102301 gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc
   102361 attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc
   102421 ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat
   102481 tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg
   102541 gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg
   102601 tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc
   102661 tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt
   102721 aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg
   102781 ataccattta attatgattc aaacaatgag gctttaaata aaaaaaggag gttatttttt
   102841 taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga agaaaatcaa
   102901 aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
   102961 tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca
   103021 gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag
   103081 aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat
   103141 ggatgacttg agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt
   103201 cactgttgag catacaggga atacgattat cgatcttgct tttaattata tccattttgc
   103261 aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga
   103321 gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt
   103381 gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg
   103441 gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat
   103501 gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat
   103561 atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt
   103621 cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga
   103681 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
   103741 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
   103801 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
   103861 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
   103921 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
   103981 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
   104041 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
   104101 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
   104161 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
   104221 ccaaaacatc attgaaaaga aaataaaacc taaagacacc tggggaatgt catttggcta
   104281 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
   104341 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
   104401 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
   104461 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
   104521 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
   104581 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
   104641 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
   104701 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
   104761 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
   104821 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
   104881 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
   104941 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
   105001 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
   105061 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
   105121 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
   105181 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
   105241 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
   105301 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
   105361 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
   105421 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
   105481 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
   105541 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
   105601 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
   105661 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
   105721 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
   105781 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
   105841 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
   105901 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
   105961 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
   106021 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
   106081 atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
   106141 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
   106201 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
   106261 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
   106321 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
   106381 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
   106441 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
   106501 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
   106561 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
   106621 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
   106681 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
   106741 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
   106801 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
   106861 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
   106921 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
   106981 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
   107041 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcgtattg
   107101 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
   107161 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
   107221 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
   107281 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
   107341 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
   107401 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
   107461 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
   107521 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
   107581 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
   107641 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
   107701 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
   107761 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
   107821 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
   107881 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
   107941 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
   108001 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
   108061 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
   108121 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
   108181 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
   108241 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
   108301 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
   108361 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
   108421 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
   108481 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
   108541 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
   108601 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
   108661 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
   108721 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
   108781 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
   108841 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
   108901 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
   108961 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
   109021 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
   109081 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
   109141 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
   109201 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
   109261 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
   109321 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
   109381 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
   109441 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
   109501 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
   109561 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
   109621 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
   109681 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
   109741 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
   109801 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
   109861 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
   109921 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
   109981 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
   110041 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
   110101 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
   110161 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
   110221 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
   110281 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
   110341 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
   110401 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
   110461 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
   110521 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
   110581 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
   110641 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
   110701 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
   110761 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
   110821 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
   110881 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
   110941 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
   111001 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
   111061 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
   111121 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
   111181 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
   111241 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
   111301 agaacttggc cggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
   111361 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
   111421 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
   111481 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
   111541 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
   111601 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
   111661 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
   111721 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
   111781 taacgagtat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
   111841 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
   111901 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
   111961 tgatcttaga aatgatgggc caaccaaaag acgcatacga ccatgtaaac gaccgcgcag
   112021 ggcacgattt gcgttatgca atcgattcga caaaattgcg agaagagcta ggttggcagc
   112081 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
   112141 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
   112201 aatacagagg aaatttcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
   112261 tctctttttt aaaataaaaa tcaaggagcg gataaccata tgtttttatt aactggagga
   112321 aatggacaat taggtacaga attgcgccat ttattagatg aaaaaaatct gccatatgtt
   112381 tcaacagatg caaaagactt ggatatcaca gatgctgaga aaacaatggc ttatattaca
   112441 gaattaaaac ccgatgttat tttccattgc gcagcttaca cagcagtaga taaagcagaa
   112501 gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt
   112561 gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta cagactatgt tttcgatgga
   112621 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
   112681 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
   112741 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
   112801 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
   112861 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
   112921 catttgtcga acgaaaatag ctgttcttgg tatgagttcg caaaagaaat attgaaagat
   112981 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
   113041 cagtattcag tcatgagcct gaaaaagaca gaagcgttag gttttgtcat accaacttgg
   113101 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
   113161 tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga
   113221 aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt gacctgtttc taaaactccg
   113281 ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc
   113341 acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat
   113401 ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct
   113461 ctcacaagct gaagaggact tttcgttggt gaacgaggat tattcattaa acggacaaaa
   113521 catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa
   113581 ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt
   113641 agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga ggaaaaaagc
   113701 tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat
   113761 gcaacatgtg ggcgatttga ttttctttac tttgggactg atgattgctt tttatcatta
   113821 tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt
   113881 cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct
   113941 gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt
   114001 ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg
   114061 gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt
   114121 aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat
   114181 tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt
   114241 tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa
   114301 gttactggga cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg
   114361 gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat
   114421 ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag
   114481 agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta
   114541 tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc
   114601 agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt
   114661 ttacctcgct tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga
   114721 aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca
   114781 agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg
   114841 agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga
   114901 tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc
   114961 taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt
   115021 tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta
   115081 caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat
   115141 gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa
   115201 tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta gtagaaagga
   115261 gagtgaagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct
   115321 tgcttttgtt gctgacgcta gtcttttttg tttggacagc aggggtaggt tttccagcaa
   115381 ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca
   115441 ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc
   115501 ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac
   115561 tgtttccttt ggttatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg
   115621 atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat
   115681 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
   115741 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
   115801 gtggatttgc cagttctttt gaacgatggt atgttcgaat catcggtggg atagcagtcg
   115861 ttgcagcctt tttaggtatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
   115921 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
   115981 ttgcctacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
   116041 aaaaagtcaa tttgtttacc gatcgagaag atatttggca taaattctat cagacgctag
   116101 gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccag cgaggaaacc
   116161 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
   116221 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
   116281 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
   116341 tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg
   116401 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
   116461 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
   116521 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
   116581 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
   116641 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
   116701 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
   116761 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
   116821 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
   116881 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
   116941 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
   117001 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
   117061 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
   117121 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
   117181 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
   117241 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
   117301 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
   117361 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
   117421 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
   117481 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
   117541 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
   117601 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
   117661 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
   117721 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
   117781 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
   117841 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
   117901 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
   117961 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
   118021 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
   118081 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
   118141 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
   118201 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
   118261 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
   118321 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
   118381 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
   118441 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
   118501 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
   118561 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
   118621 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
   118681 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
   118741 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
   118801 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
   118861 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
   118921 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
   118981 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
   119041 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
   119101 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
   119161 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
   119221 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
   119281 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
   119341 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
   119401 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
   119461 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
   119521 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
   119581 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
   119641 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
   119701 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
   119761 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
   119821 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
   119881 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
   119941 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
   120001 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
   120061 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
   120121 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
   120181 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
   120241 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
   120301 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
   120361 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
   120421 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
   120481 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
   120541 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
   120601 gttaagaccc aaagcggttt caaggtacgt ttatcaggga aaatgagagg aaaagccatc
   120661 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
   120721 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
   120781 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
   120841 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
   120901 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
   120961 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
   121021 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
   121081 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
   121141 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
   121201 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
   121261 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
   121321 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
   121381 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
   121441 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
   121501 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
   121561 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
   121621 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
   121681 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
   121741 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
   121801 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
   121861 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
   121921 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
   121981 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
   122041 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
   122101 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
   122161 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
   122221 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
   122281 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
   122341 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
   122401 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
   122461 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga cggaatcggc
   122521 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
   122581 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
   122641 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
   122701 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
   122761 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
   122821 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
   122881 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
   122941 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
   123001 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
   123061 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
   123121 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
   123181 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
   123241 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
   123301 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
   123361 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
   123421 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
   123481 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
   123541 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
   123601 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
   123661 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
   123721 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
   123781 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
   123841 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
   123901 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
   123961 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
   124021 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
   124081 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
   124141 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
   124201 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt
   124261 aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata
   124321 ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt
   124381 ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg
   124441 atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt
   124501 gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg
   124561 acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc
   124621 ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt
   124681 cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat
   124741 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
   124801 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
   124861 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
   124921 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
   124981 agtattattg aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
   125041 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
   125101 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
   125161 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
   125221 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
   125281 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
   125341 tcgatcgtag gaccacgtcc tgaacgttct ttcttcgtcg atcagttcaa tgcagaaaac
   125401 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
   125461 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
   125521 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
   125581 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
   125641 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
   125701 ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa
   125761 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
   125821 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
   125881 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
   125941 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
   126001 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
   126061 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
   126121 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
   126181 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
   126241 agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg
   126301 aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac
   126361 cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac
   126421 gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt
   126481 tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata
   126541 ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa
   126601 aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt
   126661 ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag
   126721 ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt
   126781 ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt
   126841 gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga
   126901 ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata
   126961 gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt
   127021 ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat
   127081 agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt
   127141 ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt
   127201 gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga tttagagctt
   127261 tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt
   127321 acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta
   127381 gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa
   127441 gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta
   127501 aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta
   127561 atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta
   127621 aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt
   127681 gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct
   127741 aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag
   127801 agtatatata gtagag
//
LOCUS       DACDRK010000003        94266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDRK010000003 DACDRK010000000
VERSION     DACDRK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 94266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.003"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWJ77_000236"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWJ77_000236"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9451888.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWJ77_000237"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWJ77_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9451889.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWJ77_000238"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWJ77_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9451890.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWJ77_000239"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWJ77_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9451891.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWJ77_000240"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWJ77_000240"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9451892.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWJ77_000241"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWJ77_000241"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9451893.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWJ77_000242"
     CDS             complement(8053..8523)
                     /locus_tag="IWJ77_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9451894.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWJ77_000243"
     CDS             complement(8569..8994)
                     /locus_tag="IWJ77_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9451895.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWJ77_000244"
     CDS             complement(9061..9330)
                     /locus_tag="IWJ77_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9451896.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWJ77_000245"
     CDS             complement(9341..10936)
                     /locus_tag="IWJ77_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9451897.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWJ77_000246"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWJ77_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9451898.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWJ77_000247"
     CDS             complement(14489..15049)
                     /locus_tag="IWJ77_000247"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9451899.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWJ77_000248"
     CDS             15402..16376
                     /locus_tag="IWJ77_000248"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9451900.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWJ77_000249"
     CDS             16546..17946
                     /locus_tag="IWJ77_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9451901.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWJ77_000250"
     CDS             complement(18024..18746)
                     /locus_tag="IWJ77_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9451902.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWJ77_000251"
     CDS             complement(18825..19331)
                     /locus_tag="IWJ77_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9451903.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWJ77_000252"
     CDS             19454..21253
                     /locus_tag="IWJ77_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9451904.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IWJ77_000253"
     CDS             complement(21321..22895)
                     /locus_tag="IWJ77_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451905.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IWJ77_000254"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IWJ77_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9451906.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IWJ77_000255"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IWJ77_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9451907.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IWJ77_000256"
     CDS             complement(24672..25310)
                     /locus_tag="IWJ77_000256"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9451908.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IWJ77_000257"
     CDS             complement(25311..25961)
                     /locus_tag="IWJ77_000257"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9451909.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IWJ77_000258"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IWJ77_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9451910.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IWJ77_000259"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IWJ77_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9451911.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IWJ77_000260"
     CDS             complement(29104..29844)
                     /locus_tag="IWJ77_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9451912.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IWJ77_000261"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IWJ77_000261"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9451913.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IWJ77_000262"
     CDS             complement(31215..32153)
                     /locus_tag="IWJ77_000262"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9451914.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IWJ77_000263"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IWJ77_000263"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9451915.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IWJ77_000264"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IWJ77_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9451916.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IWJ77_000265"
     CDS             complement(35313..35618)
                     /locus_tag="IWJ77_000265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9451917.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IWJ77_000266"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IWJ77_000266"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9451918.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="IWJ77_000267"
     CDS             complement(36724..37008)
                     /locus_tag="IWJ77_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451919.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="IWJ77_000268"
     CDS             complement(37026..37916)
                     /locus_tag="IWJ77_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9451920.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="IWJ77_000269"
     CDS             complement(37936..38154)
                     /locus_tag="IWJ77_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451921.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="IWJ77_000270"
     CDS             complement(38124..39824)
                     /locus_tag="IWJ77_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9451922.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="IWJ77_000271"
     CDS             40091..40864
                     /locus_tag="IWJ77_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451923.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IWJ77_000272"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="IWJ77_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9451924.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="IWJ77_000273"
     CDS             complement(41688..42659)
                     /locus_tag="IWJ77_000273"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9451925.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IWJ77_000274"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="IWJ77_000274"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9451926.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IWJ77_000275"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="IWJ77_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9451927.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="IWJ77_000276"
     CDS             complement(45614..46438)
                     /locus_tag="IWJ77_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9451928.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="IWJ77_000277"
     CDS             complement(46411..47091)
                     /locus_tag="IWJ77_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9451929.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="IWJ77_000278"
     CDS             complement(47114..48052)
                     /locus_tag="IWJ77_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9451930.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49148)
                     /locus_tag="IWJ77_000279"
     CDS             complement(48297..49148)
                     /locus_tag="IWJ77_000279"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9451931.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IWJ77_000280"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="IWJ77_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9451932.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="IWJ77_000281"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="IWJ77_000281"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9451933.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IWJ77_000282"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="IWJ77_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9451934.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IWJ77_000283"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="IWJ77_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9451935.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="IWJ77_000284"
     CDS             complement(56026..57267)
                     /locus_tag="IWJ77_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9451936.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IWJ77_000285"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="IWJ77_000285"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9451937.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59178)
                     /locus_tag="IWJ77_000286"
     CDS             complement(58294..59178)
                     /locus_tag="IWJ77_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9451938.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="IWJ77_000287"
     CDS             complement(59956..61242)
                     /locus_tag="IWJ77_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9451939.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="IWJ77_000288"
     CDS             complement(61235..62503)
                     /locus_tag="IWJ77_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9451940.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            62755..63216
                     /gene="mscL"
                     /locus_tag="IWJ77_000289"
     CDS             62755..63216
                     /gene="mscL"
                     /locus_tag="IWJ77_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9451941.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(63276..63722)
                     /locus_tag="IWJ77_000290"
     CDS             complement(63276..63722)
                     /locus_tag="IWJ77_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9451942.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(63815..64060)
                     /locus_tag="IWJ77_000291"
     CDS             complement(63815..64060)
                     /locus_tag="IWJ77_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451943.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(64176..64754)
                     /locus_tag="IWJ77_000292"
     CDS             complement(64176..64754)
                     /locus_tag="IWJ77_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9451944.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(64831..65361)
                     /locus_tag="IWJ77_000293"
     CDS             complement(64831..65361)
                     /locus_tag="IWJ77_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9451945.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(65365..66225)
                     /gene="rlmB"
                     /locus_tag="IWJ77_000294"
     CDS             complement(65365..66225)
                     /gene="rlmB"
                     /locus_tag="IWJ77_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9451946.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(66218..66613)
                     /locus_tag="IWJ77_000295"
     CDS             complement(66218..66613)
                     /locus_tag="IWJ77_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9451947.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(66610..68022)
                     /locus_tag="IWJ77_000296"
     CDS             complement(66610..68022)
                     /locus_tag="IWJ77_000296"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9451948.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(68019..68555)
                     /gene="cysE"
                     /locus_tag="IWJ77_000297"
     CDS             complement(68019..68555)
                     /gene="cysE"
                     /locus_tag="IWJ77_000297"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9451949.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(68877..70337)
                     /locus_tag="IWJ77_000298"
     CDS             complement(68877..70337)
                     /locus_tag="IWJ77_000298"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9451950.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(70507..71664)
                     /locus_tag="IWJ77_000299"
     CDS             complement(70507..71664)
                     /locus_tag="IWJ77_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9451951.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(71698..73068)
                     /gene="radA"
                     /locus_tag="IWJ77_000300"
     CDS             complement(71698..73068)
                     /gene="radA"
                     /locus_tag="IWJ77_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9451952.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(73131..73658)
                     /locus_tag="IWJ77_000301"
     CDS             complement(73131..73658)
                     /locus_tag="IWJ77_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9451953.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            73888..74694
                     /gene="proB"
                     /locus_tag="IWJ77_000302"
     CDS             73888..74694
                     /gene="proB"
                     /locus_tag="IWJ77_000302"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9451954.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            74691..75938
                     /locus_tag="IWJ77_000303"
     CDS             74691..75938
                     /locus_tag="IWJ77_000303"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9451955.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            76014..76358
                     /locus_tag="IWJ77_000304"
     CDS             76014..76358
                     /locus_tag="IWJ77_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451956.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(76475..76801)
                     /locus_tag="IWJ77_000305"
     CDS             complement(76475..76801)
                     /locus_tag="IWJ77_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451957.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(77416..78618)
                     /locus_tag="IWJ77_000306"
     CDS             complement(77416..78618)
                     /locus_tag="IWJ77_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9451958.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            78735..79250
                     /locus_tag="IWJ77_000307"
     CDS             78735..79250
                     /locus_tag="IWJ77_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9451959.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(79449..79895)
                     /locus_tag="IWJ77_000308"
     CDS             complement(79449..79895)
                     /locus_tag="IWJ77_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9451960.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(79942..81420)
                     /gene="msr"
                     /locus_tag="IWJ77_000309"
     CDS             complement(79942..81420)
                     /gene="msr"
                     /locus_tag="IWJ77_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9451961.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(81774..82403)
                     /locus_tag="IWJ77_000310"
     CDS             complement(81774..82403)
                     /locus_tag="IWJ77_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9451962.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(82387..82851)
                     /locus_tag="IWJ77_000311"
     CDS             complement(82387..82851)
                     /locus_tag="IWJ77_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9451963.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(83338..84606)
                     /locus_tag="IWJ77_000312"
     CDS             complement(83338..84606)
                     /locus_tag="IWJ77_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9451964.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(84837..85574)
                     /locus_tag="IWJ77_000313"
     CDS             complement(84837..85574)
                     /locus_tag="IWJ77_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9451965.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(85641..86210)
                     /locus_tag="IWJ77_000314"
     CDS             complement(85641..86210)
                     /locus_tag="IWJ77_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9451966.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            86338..87210
                     /locus_tag="IWJ77_000315"
     CDS             86338..87210
                     /locus_tag="IWJ77_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9451967.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            87552..88424
                     /locus_tag="IWJ77_000316"
     CDS             87552..88424
                     /locus_tag="IWJ77_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9451968.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            88421..90379
                     /locus_tag="IWJ77_000317"
     CDS             88421..90379
                     /locus_tag="IWJ77_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9451969.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(90711..91562)
                     /locus_tag="IWJ77_000318"
     CDS             complement(90711..91562)
                     /locus_tag="IWJ77_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9451970.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            91685..92152
                     /locus_tag="IWJ77_000319"
     CDS             91685..92152
                     /locus_tag="IWJ77_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9451971.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(92415..92897)
                     /locus_tag="IWJ77_000320"
     CDS             complement(92415..92897)
                     /locus_tag="IWJ77_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9451972.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(92997..93860)
                     /locus_tag="IWJ77_000321"
     CDS             complement(92997..93860)
                     /locus_tag="IWJ77_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9451973.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        26339 a        20305 c        16934 g        30688 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
    62761 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
    62821 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
    62881 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
    62941 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
    63001 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
    63061 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
    63121 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
    63181 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
    63241 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
    63301 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
    63361 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
    63421 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
    63481 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
    63541 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
    63601 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
    63661 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
    63721 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
    63781 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
    63841 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
    63901 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
    63961 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
    64021 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
    64081 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaag aaatgcaata
    64141 aaaataaata agaagctcat taagattttt ctaaattatt cctcattata aaatttcaag
    64201 tgttttttta ttttgagttt tactctcccg taagcgctca ttacttgctt gtgatccatt
    64261 gctaaacatt cagccatgtc tacaaaatca acgccttgca tataataatg aaaaatttta
    64321 gcttctattt ttgaaaactg gatttcgcaa ttttcgattg cttcttttaa taaaagatgc
    64381 tgaattgcta aaggttcttg caatgtgatc tctgctaaaa agtctggtcc ttctgtctct
    64441 attttttggt cgattgagac ttcatctttt tgatcacgac gcttcagcgc gttctgcttc
    64501 ctcaacagag aatagatttg attgcttata acgcttttat aataacttcc aaagctaaca
    64561 cctttgcttt tttcgtaagt tctcaatgcc tgatataaag cgatccttgc ttcttgttcc
    64621 caatcctcag aaacataatt tcttaaataa taggtgtttt tcaaacgtag taaaagtgga
    64681 aaatattttt gatacagggc agcaaatgct tcttcgtcac cattacgtgc agcgcgcaat
    64741 aactcctcag acataaaata attcctccat aaattcgttt atggaggaat cttatcatca
    64801 atgattcatc gaactgatta gaacgatatg ttatttattt tttctcatta aatcgtccaa
    64861 tttttgggac aattttttta actgctcttc attccatgga gaattacgtc ggaaatcaat
    64921 aaaatgcata tctttatttt cttgggctat tgttttttcc gtttttttta tcgtcttata
    64981 taattcataa gcagaagttc gtaatgctcc ttgggaaaag atcatccact gttctgctaa
    65041 atcgcttgtc gccactgtca cttgtgtcaa acggtcattc agctgaccag cgattctctc
    65101 gatataactg tcagccgttt cgtcttcttt cgtaaaaaca acatccaact ggtatttctt
    65161 ataattttgt tggatgcccg gaactagttg ggcatcgaag acaacgatga cttccattcc
    65221 ttcatatttc gcataatttg ataaacgatg taaaagagct tctcttgcat ccgccatttt
    65281 gtcctgcttt ttaagctgga caagttccgg ccacgaacca atcatgttgt agccgtctac
    65341 aatcaacagc tgttttttca tggtctactc cccctgacgt tgggaaaaag cctgatacat
    65401 aagcaaacca gcagcaacac tggcatttaa gctctggacg tgcccggtca tcgggatcgt
    65461 cagcaattca tccatttctt ttttcaatcc tggactcatt cctttgcctt cattaccaat
    65521 gactaaagca attgatcctt tggcattcca tttttggaag tttgtgccgg tcatatctgt
    65581 gccaaatacc caaaatcctg tttctttgag ctgttgcaca gcttgtacca aattcgtcac
    65641 gcgtgcaaca gggacatgtt cgactgcccc agtggaagtc tttgttacga taggtgtgat
    65701 tccaactgct cggtgttttg gtatgatcac accatcgacg ccacttgcgt ctgctgtccg
    65761 taaaatcgac ccaaaattgt gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc
    65821 ttcttttttc tcttttgttc gttcaatcag ttggtcaagg gataaatatt cataaggtgt
    65881 aatcgcaaga acgactcctt gatgcacgcc gtgatcgctc aaagtttcta atttttgttt
    65941 aggaacccat ttgactggca cagcttgttc acgtgcgatt tctttcagtt cttctacttt
    66001 ttctcctcgt gaatcttctt gaagaaagag tttattccct cgcccttgtt gtagcgcttc
    66061 tactgcggca tggtgcccaa agacgaaatt atcttctaat gcttcttcat tttgatcaga
    66121 aactgactga tttcgtccag tttgcttttt atttgaatat ctattttgtt ttttgtcttc
    66181 aaatccacgt ttcgattgat gtttgtttgt tctttcttta cccatttttt tcaccaactt
    66241 tctgaataca ccagcggatc aattcttcca tccgctcggt ttgttttgtc aaatgcagat
    66301 agcccatgag cgcttcgaac cctgttgcga cccgatacgt cgtaacatca gcattttttg
    66361 cgctcgtatg gcttttagca tttcttcctc tgcgataata aagttcttct tcttctgtca
    66421 aaagatgttc ttccagcatt tgctgaatca aaaaggcttg tgcattcgca gaaacataat
    66481 tcgttgccgc acgatgcagc ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac
    66541 gaacatagat ttcgtaaatc gcatctccaa cataagccaa ggctaaaccg ttcaattgtg
    66601 tgtaatctct catgcttctc ttctccatcg ggttccttgc ggtgtatctt ctaataagat
    66661 ccctttttct ttcaacaaat cacgaatttc gtcgcttcgg acaaaatcac gattgcttct
    66721 tgcttgattt cgttcttcga tcaattgttc aatctcttca tctaataact cttctgctgt
    66781 aaataaaata ccgaagatcg aaagccattg cgagaataaa gcttctcctt gtgccaagac
    66841 agcggcagaa actttcggct ggtctagata agtgttcagc cacttagcaa gctcatagac
    66901 cactgtgatc ccatttgctg cattaaaatc atcgtccatt tcttcgatga atcgctgttc
    66961 taaattttct aacaaagcga ttttatccgc atcatctgcc aaattctctg aggcagtttc
    67021 ttggcggaat ttcaaattgt caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc
    67081 tttcatcgtc gtttcactgt agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa
    67141 acgcaagact tgtggatcaa cgtttttgat catttcatgt acagtgatga aattccctag
    67201 agatttactc attttttcat cgtcttcgcc aatcgttaca tagccattgt gcatccagta
    67261 attagcgaat tttttccctg ttttcgcttc actttgtgcg atttcatttt catgatgagg
    67321 aaattctaaa tcttgcccgc caccatggat atcgatcgtt tctcctaaat gttttgttgc
    67381 cataacggaa cattcaatat gccaaccggg acgtcctttg ccccatggtg aatcccaaga
    67441 aatctcgcca ggttttgctt gtttccataa agcgaaatct aatgggtctt ctttttgctg
    67501 ctgttcttcc cctgtacgct gacttgctcc gacttccagt tcatcgatgg attggtcact
    67561 taatttccca taattttcaa atttacgggt acggtaatac acatcacctg aagattcata
    67621 agcatagcct ttttcaacca acacttgaat aaatgctaaa atatctggca tatgatccat
    67681 gactcttgga tgcagtgtag caggttcgac attcaacgct ttcgtatctt cttcaaatgc
    67741 tcggatgaaa cgatcagcaa cttctggtgc tgtaatacct aattcgtttg ctgtacggat
    67801 gattttatcg tctacatccg tgaaattcga tacgaaatcg actttatagc ctcgatattc
    67861 aaaatagcgt cgaattgtat cgaaagcaat ggtgctacga gcatttccga tatggatata
    67921 attatacaca gtcggtccgc agacatacat accgacatgt cccttttgta tagggatgaa
    67981 ttcttctttt tttctggtca atgtattgta gatttttatc attctgatct cctcactttt
    68041 tctcctttga ttcgaacgac ttttgctggt actcctaccg ctgtagcgtc gtctggaaca
    68101 tctttcaaca caacggcacc tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct
    68161 aatatctgcg cattcgcaga gagcatggct ccttgtttaa cagttgggtg tcttttaccc
    68221 gtttcttttc ctgtgccgcc taatgttacg ccatggaata aaatcacatc gtcctcgatt
    68281 tctgcggttt cgccaatgac gattcccatt ccatggtcga tgaacacgcc agttccaatc
    68341 gttgctcctg gatggatctc aacaccagtt aggaaacgcc agaattgagc attgatcttc
    68401 gccaataaaa agagccggtg ctgatataaa aaatgagaga aacgatgcca aaacaaggca
    68461 tgaaaaccag ggtatgtcaa aacgatctca gctgtcgaac gtgcagccgg atcgtttctc
    68521 tttgctgcat caatcgctcg ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat
    68581 tttatgtagg aaatcatctt cttataaaga aaagaagcgc cttttaaata agtttcctta
    68641 tcaaaagacg ctttccgcgt ggttccactt ttcttcataa tcaatgatta tctctgacag
    68701 ctaacgcctg ttatacgttc ttggctactt tgtttcctat aaaacagttt ttcctgtttt
    68761 ctgtgtttcg ccaagaccac tcccagaggc atttcggcaa ccattcattg ataccttgca
    68821 ccacccggta tctctcttgt aatgatcagc tgcttacttt tttctgttca tcgtgtttag
    68881 ttcagtactt gatttaaatg tgctaatgat ttttcacgac caagcaattc gatcgtttca
    68941 ggcaattctg gaccatgcat ttgtcctgat acagctacac ggattggcat gaaaagattt
    69001 tttcctttta cgccagtttc tttttgaaca gctttgatgg cagctttgac agttggagca
    69061 tctactactt ccatttgttc tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt
    69121 tcaccagcaa gtacttcttt tgctgcttca ttcaatacag gatgttcatt gaaaaatagc
    69181 gtagacagat caacgatttc tgcagcatag ctcatttgag gttgatacag gctaacgatc
    69241 ttcttcaccc attcgattgt cttttcatct gggttttctg ctacacgtcc atcagcgatt
    69301 agataaggca gacacatgtc agtaaatgca tctagatcca ttgctttgat gtaatggttg
    69361 tttacccatt gcagtttttt cgcatcgaaa gctgcgggtg atttacttag gcgttttgga
    69421 tcgaacattt tgatcaattc ttggtgttcg aagatttcgt cttcgcccac aggtgaccag
    69481 cccaataatg caataaagtt gaacattgca tctggtagat agcctaattc gcgatattgt
    69541 tcgataaatt gcaagattga ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg
    69601 atgatcaatg tcatatgtcc gaaggttggt ggtgtccagc caaatgcttc atagatcatc
    69661 agttgttttg gtgtattggc aatatggtca tctccacgta aaacgtgagt gattttcatc
    69721 aaatgatcat cgaccgcaac agcaaaatta taagttggca taccatcgcg tttttggatg
    69781 acaaagtcgc caccgatatt atcggattcg aacgaaatag cgcctttcac catatctgta
    69841 aatgcgtatt ctgtattgcg aggcacacgg aaacgtacaa cagattctag accttgtgct
    69901 tctttttctg cttgttcttc aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt
    69961 tctcctcttg cgcgttgtgc ttcccgttct gcttctaatt cttcttctgt gcaatagcat
    70021 ttgtatgctc gattgctagc aagcagctga tcaatcagcg gttgatagat ttctttacgt
    70081 tcagattgac ggtaagggcc gtattctcca ggattttcag gagattcatc ccaattcatt
    70141 cccaaccaag caaggttttc caactggctt ttttcgccat cttcgatatt acgtttttgg
    70201 tcagtatcct caatacgaat aataaagtct ccatcattgt ggcgggcaaa caaataattg
    70261 aataatgcag ttcttgcatt tccaatatgc aggtgcccag ttggacttgg tgcatagcgt
    70321 acgcgtactt tcgtcatttt cttcctcttc tttcaaacta gactttgact aacagcaaaa
    70381 attccgtcag ttacggcttc tcaaatatca tttatttaaa gccactcagc acaaatttta
    70441 ccttttctaa ggtaaatagt aaagccaact ggttgaattt ccttaccagt tggctgaatt
    70501 tcttttttat tttgattgtt tagtcttttt ctctgtctct tttttattgt cgattcctcg
    70561 tcctgcatgt gcaggtttag caaagatcat gcgtccagca gctgtttgaa gggcacttgt
    70621 tacaacgact tcgatacgtt cattcatgta atgctgtcca tcttctacga caaccatcgt
    70681 gccgtcatca aggtaggcta ctccttgttg gcgttctgtc ccggctttta ctactagaac
    70741 attcatcgtt tcaccaggga tgacgactgg ttttacggca tttgccaacg cattgatatt
    70801 aagaacaggt acattttgga attcagagac tttattcaag ttatagtcat tggttacaat
    70861 gacaccatct aaaagcttag ccaatttgat caatttgctg tctacttcac tgatatcttc
    70921 aaagtctcca tcgtacattt cgacagatat accctcttct ttttgcaagg catttaggat
    70981 atctaatccg cgacgaccac gcacacgttt caaactatcc cctgagtccg cgatgtattg
    71041 tagttcgtaa agaacaaaat tagggatcat caaggttcct tctaagaatc cagtctttgt
    71101 aatatcataa atccgaccgt caatgatcac gcttgtatcc aagattttat atttatggaa
    71161 attttctcct gattttcgtt ctaacagttg actctcaact tgactagctg tttcttgctc
    71221 gatttttttg cttcgtgaac caaaaacttt cttccattct tcgattcgtg tcgttcccat
    71281 tctcaatccc agatagccta agatgatcat cagtaaaatc ggcaatacac tattgacgaa
    71341 tggaatttcc atattgtaaa gaggggtcga gatcacaaca cctaaagtca atccgatgat
    71401 cgcaccgatc gcaccgaaga gaagataagt caagctgacc tcacttagtt tttgttctac
    71461 ttgtttgata atagctgaga tatgtttggc caacagcaaa gataataaaa agaaaataag
    71521 tgcgcctaac aggctgttgg taaatgtatt attcagccat ccgtttgact gttgaccgat
    71581 tgcctgccaa gctgagggta aaaatgaaat acccaagctt gcgccgacaa tgatcatcag
    71641 caaagtaatg acacgttttt gcatcgtttt tcctccgttt cgacaatcat ttattagtta
    71701 gcggaacaca cgcttcaacg tttcagccaa agttgctact ccaactatct cgattccttt
    71761 tggcgcttcc cagttaccta agttgttttt aggtaggtat acttttgtga agccgagttt
    71821 ttggacttct ttgactcgtt gttcaatatg actgacacgt ctgatttctc cagtcagtcc
    71881 aatttcaccg atgaaacatt ccgttggctg tgttcctttt tctttatagc ttgaagcaat
    71941 actgacagcg atagctaaat caatcgctgg ttcatttaat tttaccccac ctgcagcttt
    72001 taagtaagca tcttgattct gaagaagcaa cccagctctt ttttccagca ctgccatgat
    72061 cagagatacc cgattgaaat caagacctgt tgttgttcgt ttggcatttc cgaacatagt
    72121 aggtgtgact aatgcttgga tctctaccaa aattggacgt gtcccttcca ttgccacaac
    72181 gatagcagag ccagttgcac cgtctaggcg ttcttctaag aaaatctgtg aaggattagc
    72241 gacttcttcc aatccatgct catgcatttc aaaaatccca atctcattcg ttgagccaaa
    72301 tcggtttttt actgctcgca aaatccgaaa actatgatgt tgttcacctt caaaatataa
    72361 caccgtatcc accatatgtt ctaacattct tgggccggcg atcgagcctt ctttggtaac
    72421 atgaccgacg ataaaaatgg ctatcccatt tgttttagcg gttttgagta gttctgcagt
    72481 ggtttctctt acttggctga cacttcctgc cacacttgtg atgtctggct gcgtcatcgt
    72541 ttggatggaa tcaatgataa cataatcagg ggagatatgt tcgatggcac gactgatttc
    72601 gttcatatct gtttcagcgt atagataaaa ttctgtgttg atacttccga ggcgttccgc
    72661 gcgtaatttt atctgctctg cgctttcttc gccggataca tataagactt ttccgcctat
    72721 ggctgctaat tgctgagaaa cttgtaacag caaagtggat ttcccgatac ctggatcgcc
    72781 accaatcaag accatagacc ctggcaccac acctccgcct aacacacggt tcagttcttc
    72841 taattttgtt ttgatccgag gttctttttt agggacgacg tctgcgattt ttgttggttt
    72901 tgctttttct cctgtcaaac ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc
    72961 ttcgaccatc gtattccatt ttccgcaatt tgggcagcgt cccaagaact taggcgacac
    73021 atacccgcaa ttttggcaaa caaattgtac ttttgatttt tttgccattg taccgcctcc
    73081 ttttatccta atcatgagac attaaatcaa gcttaagtat ctttaacgtt ttatttactg
    73141 gaagaaccaa atccgccttg acgttgagca gtcgtttgat cttgatctgc ttttaaaaac
    73201 ggcaaaaaga tcccttggcc gatacgctcg ccttttttga tcactacatc cttgaaacca
    73261 aagttggtga attggaacat gatatggcct tcatttgcct cattattgta ataatcactg
    73321 tcaatcacac ctactccgtt tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg
    73381 ttagcaagct gcaaaaattc atcttctccc atgtaagctt tgattccagt aggcacgagg
    73441 gttggtttca attgtttttc attttctgat acatcttttt tcaatccatc tgcgactgtt
    73501 tccaacccct ttttccagcc tgctttccag atacttggaa tcacgacctc ttctgctgct
    73561 tcaaaatcat agccagcagc atgatgtgta gcgcgctgcg gtagattgat attttgatct
    73621 ttatatttag aaataacttc aaatcctctt tttctcaccg gttttcctcc ctaaataaac
    73681 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
    73741 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
    73801 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
    73861 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
    73921 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
    73981 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
    74041 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
    74101 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
    74161 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
    74221 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
    74281 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
    74341 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
    74401 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
    74461 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
    74521 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
    74581 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
    74641 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
    74701 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
    74761 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
    74821 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
    74881 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
    74941 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
    75001 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
    75061 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
    75121 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
    75181 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
    75241 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
    75301 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
    75361 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
    75421 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
    75481 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
    75541 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
    75601 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
    75661 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
    75721 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
    75781 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
    75841 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
    75901 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
    75961 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
    76021 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
    76081 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
    76141 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
    76201 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
    76261 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
    76321 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
    76381 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
    76441 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
    76501 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
    76561 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
    76621 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
    76681 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
    76741 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
    76801 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
    76861 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
    76921 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
    76981 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
    77041 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
    77101 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
    77161 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
    77221 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
    77281 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
    77341 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
    77401 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
    77461 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
    77521 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
    77581 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
    77641 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
    77701 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
    77761 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
    77821 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
    77881 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
    77941 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
    78001 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
    78061 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
    78121 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
    78181 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
    78241 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
    78301 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
    78361 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
    78421 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
    78481 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
    78541 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
    78601 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
    78661 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
    78721 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
    78781 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
    78841 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
    78901 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
    78961 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
    79021 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
    79081 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
    79141 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
    79201 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
    79261 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
    79321 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
    79381 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
    79441 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
    79501 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
    79561 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
    79621 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
    79681 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
    79741 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
    79801 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
    79861 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
    79921 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
    79981 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
    80041 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
    80101 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
    80161 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
    80221 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
    80281 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
    80341 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
    80401 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
    80461 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
    80521 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
    80581 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
    80641 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
    80701 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
    80761 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
    80821 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
    80881 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
    80941 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
    81001 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
    81061 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
    81121 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
    81181 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
    81241 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
    81301 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
    81361 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
    81421 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
    81481 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
    81541 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
    81601 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
    81661 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
    81721 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
    81781 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
    81841 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
    81901 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
    81961 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
    82021 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
    82081 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
    82141 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
    82201 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
    82261 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
    82321 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
    82381 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
    82441 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
    82501 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
    82561 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
    82621 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
    82681 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
    82741 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
    82801 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
    82861 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
    82921 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
    82981 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
    83041 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
    83101 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
    83161 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
    83221 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
    83281 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
    83341 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
    83401 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
    83461 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
    83521 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
    83581 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
    83641 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    83701 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    83761 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    83821 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    83881 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    83941 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    84001 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    84061 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    84121 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    84181 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    84241 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    84301 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    84361 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    84421 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    84481 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    84541 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    84601 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    84661 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    84721 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    84781 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    84841 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    84901 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    84961 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    85021 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    85081 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    85141 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    85201 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    85261 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    85321 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    85381 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    85441 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    85501 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    85561 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    85621 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    85681 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    85741 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    85801 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    85861 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    85921 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    85981 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    86041 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    86101 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    86161 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    86221 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    86281 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    86341 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    86401 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    86461 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    86521 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    86581 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    86641 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    86701 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    86761 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    86821 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    86881 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    86941 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    87001 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    87061 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    87121 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    87181 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    87241 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    87301 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    87361 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    87421 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    87481 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    87541 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    87601 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    87661 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    87721 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    87781 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    87841 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    87901 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    87961 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    88021 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    88081 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    88141 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    88201 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    88261 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    88321 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    88381 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    88441 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    88501 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    88561 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    88621 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    88681 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    88741 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    88801 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    88861 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    88921 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    88981 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    89041 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    89101 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    89161 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    89221 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    89281 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    89341 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    89401 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    89461 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    89521 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    89581 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    89641 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    89701 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    89761 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    89821 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    89881 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    89941 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    90001 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    90061 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    90121 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    90181 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    90241 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    90301 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    90361 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    90421 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    90481 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    90541 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    90601 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    90661 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    90721 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    90781 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    90841 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    90901 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    90961 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    91021 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    91081 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    91141 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    91201 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    91261 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    91321 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    91381 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    91441 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    91501 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    91561 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    91621 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    91681 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    91741 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    91801 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    91861 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    91921 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    91981 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    92041 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    92101 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    92161 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    92221 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    92281 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    92341 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    92401 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    92461 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    92521 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    92581 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    92641 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    92701 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    92761 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    92821 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    92881 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    92941 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    93001 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    93061 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    93121 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    93181 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    93241 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    93301 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    93361 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    93421 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    93481 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    93541 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    93601 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    93661 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    93721 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    93781 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    93841 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    93901 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    93961 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    94021 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    94081 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    94141 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    94201 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    94261 ataaaa
//
LOCUS       DACDRK010000004        81116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDRK010000004 DACDRK010000000
VERSION     DACDRK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.004"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..361
                     /locus_tag="IWJ77_000322"
     CDS             <1..361
                     /locus_tag="IWJ77_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451974.1"
                     /translation="GTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVI
                     LMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQF
                     GLTVLGICTFIITVINR"
     gene            complement(601..684)
                     /locus_tag="IWJ77_000323"
     tRNA            complement(601..684)
                     /locus_tag="IWJ77_000323"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(648..650),aa:Leu,seq:gag)
     gene            1067..2284
                     /locus_tag="IWJ77_000324"
     CDS             1067..2284
                     /locus_tag="IWJ77_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9451975.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2468..3304
                     /locus_tag="IWJ77_000325"
     CDS             2468..3304
                     /locus_tag="IWJ77_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9451976.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3380..4528
                     /gene="nagA"
                     /locus_tag="IWJ77_000326"
     CDS             3380..4528
                     /gene="nagA"
                     /locus_tag="IWJ77_000326"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9451977.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4748..5806
                     /locus_tag="IWJ77_000327"
     CDS             4748..5806
                     /locus_tag="IWJ77_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9451978.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            5825..6844
                     /locus_tag="IWJ77_000328"
     CDS             5825..6844
                     /locus_tag="IWJ77_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9451979.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(6946..7662)
                     /locus_tag="IWJ77_000329"
     CDS             complement(6946..7662)
                     /locus_tag="IWJ77_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9451980.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(7631..8416)
                     /locus_tag="IWJ77_000330"
     CDS             complement(7631..8416)
                     /locus_tag="IWJ77_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9451981.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8407..9156)
                     /locus_tag="IWJ77_000331"
     CDS             complement(8407..9156)
                     /locus_tag="IWJ77_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9451982.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9454..9978
                     /locus_tag="IWJ77_000332"
     CDS             9454..9978
                     /locus_tag="IWJ77_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451983.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            9994..11493
                     /locus_tag="IWJ77_000333"
     CDS             9994..11493
                     /locus_tag="IWJ77_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9451984.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11581..12183)
                     /locus_tag="IWJ77_000334"
     CDS             complement(11581..12183)
                     /locus_tag="IWJ77_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9451985.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12287..13243
                     /locus_tag="IWJ77_000335"
     CDS             12287..13243
                     /locus_tag="IWJ77_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9451986.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13548..14504)
                     /locus_tag="IWJ77_000336"
     CDS             complement(13548..14504)
                     /locus_tag="IWJ77_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9451987.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14678..15238
                     /locus_tag="IWJ77_000337"
     CDS             14678..15238
                     /locus_tag="IWJ77_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451988.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15231..16055
                     /locus_tag="IWJ77_000338"
     CDS             15231..16055
                     /locus_tag="IWJ77_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9451989.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16065..16682
                     /locus_tag="IWJ77_000339"
     CDS             16065..16682
                     /locus_tag="IWJ77_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9451990.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16700..17383
                     /locus_tag="IWJ77_000340"
     CDS             16700..17383
                     /locus_tag="IWJ77_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9451991.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18066..19301
                     /locus_tag="IWJ77_000341"
                     /pseudo
     CDS             18066..19301
                     /locus_tag="IWJ77_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            19309..20196
                     /locus_tag="IWJ77_000342"
     CDS             19309..20196
                     /locus_tag="IWJ77_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9451992.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20213..21049
                     /locus_tag="IWJ77_000343"
     CDS             20213..21049
                     /locus_tag="IWJ77_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9451993.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21052..21675
                     /locus_tag="IWJ77_000344"
     CDS             21052..21675
                     /locus_tag="IWJ77_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9451994.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21672..22964
                     /locus_tag="IWJ77_000345"
     CDS             21672..22964
                     /locus_tag="IWJ77_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9451995.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23323..23420
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23536..24117
                     /locus_tag="IWJ77_000346"
     CDS             23536..24117
                     /locus_tag="IWJ77_000346"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9451996.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24120..25427
                     /locus_tag="IWJ77_000347"
     CDS             24120..25427
                     /locus_tag="IWJ77_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9451997.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            25720..26208
                     /gene="purE"
                     /locus_tag="IWJ77_000348"
     CDS             25720..26208
                     /gene="purE"
                     /locus_tag="IWJ77_000348"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9451998.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26192..27325
                     /gene="purK"
                     /locus_tag="IWJ77_000349"
     CDS             26192..27325
                     /gene="purK"
                     /locus_tag="IWJ77_000349"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9451999.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27338..28633
                     /gene="purB"
                     /locus_tag="IWJ77_000350"
     CDS             27338..28633
                     /gene="purB"
                     /locus_tag="IWJ77_000350"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9452000.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(28813..29190)
                     /locus_tag="IWJ77_000351"
     CDS             complement(28813..29190)
                     /locus_tag="IWJ77_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452001.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29446..30174
                     /locus_tag="IWJ77_000352"
     CDS             29446..30174
                     /locus_tag="IWJ77_000352"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9452002.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30174..30428
                     /gene="purS"
                     /locus_tag="IWJ77_000353"
     CDS             30174..30428
                     /gene="purS"
                     /locus_tag="IWJ77_000353"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9452003.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30430..31101
                     /gene="purQ"
                     /locus_tag="IWJ77_000354"
     CDS             30430..31101
                     /gene="purQ"
                     /locus_tag="IWJ77_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9452004.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31102..33324
                     /gene="purL"
                     /locus_tag="IWJ77_000355"
     CDS             31102..33324
                     /gene="purL"
                     /locus_tag="IWJ77_000355"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9452005.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33309..34748
                     /locus_tag="IWJ77_000356"
     CDS             33309..34748
                     /locus_tag="IWJ77_000356"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9452006.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            34780..35823
                     /locus_tag="IWJ77_000357"
     CDS             34780..35823
                     /locus_tag="IWJ77_000357"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9452007.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            35820..36398
                     /gene="purN"
                     /locus_tag="IWJ77_000358"
     CDS             35820..36398
                     /gene="purN"
                     /locus_tag="IWJ77_000358"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9452008.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36395..37930
                     /gene="purH"
                     /locus_tag="IWJ77_000359"
     CDS             36395..37930
                     /gene="purH"
                     /locus_tag="IWJ77_000359"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9452009.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            37946..39220
                     /gene="purD"
                     /locus_tag="IWJ77_000360"
     CDS             37946..39220
                     /gene="purD"
                     /locus_tag="IWJ77_000360"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9452010.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39342..40256
                     /locus_tag="IWJ77_000361"
     CDS             39342..40256
                     /locus_tag="IWJ77_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9452011.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40389..41954)
                     /locus_tag="IWJ77_000362"
     CDS             complement(40389..41954)
                     /locus_tag="IWJ77_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9452012.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(41947..43398)
                     /locus_tag="IWJ77_000363"
     CDS             complement(41947..43398)
                     /locus_tag="IWJ77_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9452013.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43412..44428)
                     /locus_tag="IWJ77_000364"
     CDS             complement(43412..44428)
                     /locus_tag="IWJ77_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9452014.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            44648..45736
                     /locus_tag="IWJ77_000365"
     CDS             44648..45736
                     /locus_tag="IWJ77_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9452015.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            45790..47316
                     /locus_tag="IWJ77_000366"
     CDS             45790..47316
                     /locus_tag="IWJ77_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9452016.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47318..48523
                     /locus_tag="IWJ77_000367"
     CDS             47318..48523
                     /locus_tag="IWJ77_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9452017.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            48780..49757
                     /gene="guaC"
                     /locus_tag="IWJ77_000368"
     CDS             48780..49757
                     /gene="guaC"
                     /locus_tag="IWJ77_000368"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9452018.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50021..51445
                     /locus_tag="IWJ77_000369"
     CDS             50021..51445
                     /locus_tag="IWJ77_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9452019.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            51906..53015
                     /gene="pdhA"
                     /locus_tag="IWJ77_000370"
     CDS             51906..53015
                     /gene="pdhA"
                     /locus_tag="IWJ77_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9452020.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53018..53995
                     /locus_tag="IWJ77_000371"
     CDS             53018..53995
                     /locus_tag="IWJ77_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9452021.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54020..55663
                     /locus_tag="IWJ77_000372"
     CDS             54020..55663
                     /locus_tag="IWJ77_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9452022.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            55671..57077
                     /gene="lpdA"
                     /locus_tag="IWJ77_000373"
     CDS             55671..57077
                     /gene="lpdA"
                     /locus_tag="IWJ77_000373"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9452023.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57239..57514
                     /locus_tag="IWJ77_000374"
     CDS             57239..57514
                     /locus_tag="IWJ77_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9452024.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57520..58287
                     /locus_tag="IWJ77_000375"
     CDS             57520..58287
                     /locus_tag="IWJ77_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9452025.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58455..60284
                     /gene="typA"
                     /locus_tag="IWJ77_000376"
     CDS             58455..60284
                     /gene="typA"
                     /locus_tag="IWJ77_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9452026.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60545..62317
                     /gene="recQ"
                     /locus_tag="IWJ77_000377"
     CDS             60545..62317
                     /gene="recQ"
                     /locus_tag="IWJ77_000377"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9452027.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62431..62733
                     /locus_tag="IWJ77_000378"
     CDS             62431..62733
                     /locus_tag="IWJ77_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9452028.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            62736..63899
                     /locus_tag="IWJ77_000379"
     CDS             62736..63899
                     /locus_tag="IWJ77_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9452029.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            63925..67353
                     /locus_tag="IWJ77_000380"
     CDS             63925..67353
                     /locus_tag="IWJ77_000380"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9452030.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67374..68498
                     /locus_tag="IWJ77_000381"
     CDS             67374..68498
                     /locus_tag="IWJ77_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452031.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68488..68925
                     /locus_tag="IWJ77_000382"
     CDS             68488..68925
                     /locus_tag="IWJ77_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9452032.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            68909..69247
                     /locus_tag="IWJ77_000383"
     CDS             68909..69247
                     /locus_tag="IWJ77_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9452033.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69366..69926
                     /gene="rsmD"
                     /locus_tag="IWJ77_000384"
     CDS             69366..69926
                     /gene="rsmD"
                     /locus_tag="IWJ77_000384"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9452034.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            69923..70414
                     /gene="coaD"
                     /locus_tag="IWJ77_000385"
     CDS             69923..70414
                     /gene="coaD"
                     /locus_tag="IWJ77_000385"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9452035.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70404..71447
                     /locus_tag="IWJ77_000386"
     CDS             70404..71447
                     /locus_tag="IWJ77_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9452036.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            71606..72334
                     /locus_tag="IWJ77_000387"
     CDS             71606..72334
                     /locus_tag="IWJ77_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9452037.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72450..72695
                     /locus_tag="IWJ77_000388"
     CDS             72450..72695
                     /locus_tag="IWJ77_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9452038.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            72692..72910
                     /locus_tag="IWJ77_000389"
     CDS             72692..72910
                     /locus_tag="IWJ77_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9452039.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73046..73585
                     /locus_tag="IWJ77_000390"
     CDS             73046..73585
                     /locus_tag="IWJ77_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9452040.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73578..75851
                     /locus_tag="IWJ77_000391"
     CDS             73578..75851
                     /locus_tag="IWJ77_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9452041.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            75889..76935
                     /gene="holA"
                     /locus_tag="IWJ77_000392"
     CDS             75889..76935
                     /gene="holA"
                     /locus_tag="IWJ77_000392"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9452042.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77024..77278)
                     /locus_tag="IWJ77_000393"
     CDS             complement(77024..77278)
                     /locus_tag="IWJ77_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9452043.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(77678..77947)
                     /locus_tag="IWJ77_000394"
     CDS             complement(77678..77947)
                     /locus_tag="IWJ77_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452044.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78417..78872)
                     /locus_tag="IWJ77_000395"
     CDS             complement(78417..78872)
                     /locus_tag="IWJ77_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9452045.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79010..80938)
                     /locus_tag="IWJ77_000396"
     CDS             complement(79010..80938)
                     /locus_tag="IWJ77_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9452046.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        26309 a        13954 c        17571 g        23282 t
ORIGIN      
        1 aggaacgaaa ctgtcacttt tttatccagg tacttggata gctactttag tagtcttggc
       61 attattacta tgtttaattg ttggaattta tttaagtttt gacaataaga aaacttctat
      121 ttttttagtt attttgatga taatgggatt ttgttcaagg ttaattatgg gattttctcc
      181 cacagtatgg gcttccggaa tgcggacgta ctatatactt tatgttgtta tcgccatctt
      241 agtactaatg gcagttaaag agctaatgaa aagcatgagt gtacaaaaaa atgaatttat
      301 gcaatttgga ttaaccgtgc ttggtatttg tacctttata ataacggtga taaacagata
      361 atatacaaaa aatccctcgc tttagtatta ggtgagggat tttactcgtt tttcgattaa
      421 ttgttttaac acttgcaagt ttctacgagt agcttgtttc cgaccttcat atactcacct
      481 caggctgttt acatgattgt acgttctttt tgtaaaggat acagtgatat gtatataatg
      541 gcataaagaa acgttcaaac aaaaaaactc gtgaagcaaa attaacttca cgagttgtag
      601 tgcagatgat gggactcgaa cccacacgag ctaacgctca catgaccctc aatcatgctt
      661 gtctgccaat tccaacacat ctgcgtaata ataacacact aatgatagcg aggttttaca
      721 tagttgtcaa gctcaaaaat gggaggactt attaagatgg agaaatatga cggagaattt
      781 tcaggactgg gaatgatctt aggtgtattg attggacttg cattcggacg ttttttgctt
      841 ggttttatgc taggaatcat ttgtggaatt gcaatggatt gggcagcgaa tctatggaat
      901 gactatcatg acaattaagt tctcaacgct tgtttgtgtt tttttaatac ttacgttttt
      961 cctaaataag tggcaaaatt ttctaggatg gagtatgata ttgaaatagt aaataacaaa
     1021 aaatttggaa agttctaact ttttgaaatg agggattggc taagtgatga gagaatttga
     1081 aaaatccaac aaattagaag gtgtcagtta cgatgttcgc ggtcccgttc tagaagaagc
     1141 ggagcggatg caagaagagg gcattagtat tttaaaattg aacacgggaa atcccgcacc
     1201 ttttggattt gaagcaccaa acgaagtcat tcgtgatttg atcatgaatg cccgtgactc
     1261 agaaggctac tcagattcca aggggatctt ttctgctaga aaagcaattg agcaatattg
     1321 ccaactgaaa cattttccga atgtgacgat caatgatatc tacaccggca acggagtgag
     1381 cgaattgatc acgatgtgta tgcaggggtt gctggacaat ggagatgaag tactggttcc
     1441 aatgccggac tatccgttgt ggacggcttc tattgcatta gctggaggca cacctgttca
     1501 ttatatttgc gatgaagagg cagaatggta tcctgatatt gatgatatca aatcaaaaat
     1561 aacgagtcgg acaaaagcta ttgttatcat taatcctaat aatcctactg gggcacttta
     1621 tccaaaagaa ttattagaag aaatcgttga agtagctcga caaaataacc tgattattta
     1681 ttccgacgaa atctatgatc gcttagtgat ggatggtcta gaacatattc cgattgctac
     1741 attagcgcct gatttgttcg ttgttacgct gaacggtcta tccaaatctc atcgtgtagc
     1801 aggatttcga gtaggctgga tggtcctaag tggagataag tcacacgtca aaggctatat
     1861 agaagggctg aatatgttgt cctccatgcg tttgtgttcc aatgtattgt ctcagcaaat
     1921 tatccaaacc gcgctaggcg gttatcaaag tgtcgatgag ctgctcctgc caggcggaag
     1981 gatctatgaa caaagagaat atatctataa tgcaatcaat gatattcctg gtctttcagc
     2041 cgtaaaacca aaagctgctt tttatatatt cccaaaaatt gatacaaagc gattcaatat
     2101 tttggatgat gaaaaattcg ttttggactt tttacatgaa catcatattc tacttgtaca
     2161 tggtggtgga tttaattgga accagccaga tcattttcga atcgtttatc ttcctaaaat
     2221 ggatgatttg aaacaaacag ctaagaaaat gagagaattc ctagcaacct atcgccaaaa
     2281 atgattttaa aaaccaaatt tttcagtagg agggccacag acaaacacac tgaggctctc
     2341 tttctagtgg agttttctta aataactaaa gtatattatt tagttgcaag tcttttcttg
     2401 aagtttgcga cttcctaaag taaaatacga atagaatgcg aatacgcata ttttggggag
     2461 gatacttatg gaaacgttaa tcgagtcctt tggaacgatc attaaagaga ttcgaaaaga
     2521 acaaaaaatg acgcaaaaaa tgctttcgca tgatatttgt tcacaaagtg tattaagtcg
     2581 gatcgaaaat aatgaggaac tgccgaatgt attggtgatg atgcagattt gtcaacggct
     2641 aggggtaacg attgatcatg tgatgaatct ctctcgaaaa actactcgga caaacgataa
     2701 attatttgaa ctgttggata gttgtttcca aaaacgtgat tatcggaagt tagaacaagt
     2761 actaaaatct tctgatattt ctgaaaatct gtatcaagca actgactttc aacgctatta
     2821 ttattatctc ggtgtttgcg aattcacgtt gcgtcaaaat atctctcagt ccctttatta
     2881 tttgaaagaa gcattaagct actctagcca aaaagaaaga atccatgttt ctgacacaga
     2941 aatacaactg attagttgta tcggaaaaat atatggtgtg gcaggaaaaa cggctgaagc
     3001 acgctactat ttgagtcgaa gcatccaact gctgcatcaa tcagctgatg aacgatcaaa
     3061 aagtgaattt tcgcaaattt tttataatta tggaaatttc ttattccatc aaaatgaaat
     3121 cgacgaagcg ttagaacagg tgaatcaagg aatctattgg gctcaagaga agaatagcta
     3181 ctattacctc aatgatttat ttgtgcttaa aagcttaata tacaagcgta aagaagaacc
     3241 agaaagagca gcattctatg aaggcttagc acaagctgtg aaacaaattt caaaaaattt
     3301 gtaaatggtg tagaccattc ttgacttctt ctctaaataa gagtacttta gaggtagaaa
     3361 aacaaaggga gagaatagaa tgagaacttt tatctatgct gacaaattct ttttagcgtc
     3421 aggtgtcaaa catgcaggct atcttgaaat taccgatgga ctttttggtg aatacaccaa
     3481 agaactacct gagggagaag tcaacatcat tgaccaaagc ggcaaatgga ttgctcctgg
     3541 tttggtagat acacatattc acggctatat gaatcatgat gtaatggata atgatgcaga
     3601 aggcatcaaa gtgatgtctg aaggtctgtt atcttgtgga gtgacttcat ttttaccaac
     3661 aacattgacg tcaagcaaag aacgtttaaa agatgtagct gagacaatcg gcaaaatgta
     3721 tcaggaagca ccaggagcta aaatcaaagg aatctatttt gaaggtcctt tcttcacaga
     3781 agaacacaaa ggggcacaaa acccaagtta tttcggggat cctgatttgg atactttcca
     3841 tgaatggcaa gaagcttcag gtggtttaat caaaaaaatt gctttagcac cggaacgtca
     3901 aggtgtagaa gagtttgtca aacaagtgac tgaagaagga gtagttgttt ctctaggaca
     3961 cagtaatgct acactagaag aagcgcaaaa agctgttgaa gcaggcgcaa gtgtatttgt
     4021 ccatgctttc aatggtatgc gtggattgaa tcatcgcgaa ccaggtatgg tgggtgcgtt
     4081 attatcttta gaacatgtct tttctgaatt gatttgtgat ggccatcatg tacatccaaa
     4141 tgctgcggga atcctaatgg aaaaagcagg acatgatcat gtcgcattaa ttacagactg
     4201 tatgatggcc ggtgggatgc ctgatggaaa ttacaattta ggcgaatttc cagtagtagt
     4261 aaaagaagga acagcacgtc tagaatctgg caacttagct ggaagtatat tgaaattgaa
     4321 agaagcaatt aaaaatgttg tagactgggg attagcaaca ccagaacaag cagtcatgat
     4381 ggctagtctg attccagcaa tcagctgtaa aatcgatgat acatgtggaa aaatcgctca
     4441 aggacgcgat gcagacttta tcgtattgaa ccctgatatg acattggaag caacttatct
     4501 agatggcgtg gaacgtttcc acgcataata taatttaagt taaccttaaa actaaacttc
     4561 ttaagtttgg ttttaaggtt tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa
     4621 aagtttcttt aatttcctat tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt
     4681 taatacgtat tagcaaaata aaagcttttt ttattgtgaa taaatgaaca taattaggag
     4741 gagaaagatg aacttatttg acatttttcg atcgactttg acagatatgt cgattgtcag
     4801 tgcaatcact tcaacagttt ttattatatt attagggttt gtttgtagaa aaaaggggat
     4861 tttttctgaa tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc
     4921 actggcattt cgagctttca tgcaagatat caatacagaa ttactgatgc aaggaatcaa
     4981 tgtcttgatt tggggattag ttatttatat tattcttatt ctagtttcaa aacctttttt
     5041 cttaagctat aaaggggata agcaaacaac gttgcgtgtc ctgacgatct ttggttctac
     5101 gacctttttc ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc
     5161 ctcaattttc aatattggct accgaatctt tttgtattct tatggataca taaaaatgag
     5221 tggcttgaag atggaagcta aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc
     5281 tacttttgcc ggccttttca tttggctgtt ccaagggtat ctgccacaag tgtcagttac
     5341 aggtgcagaa ggagagataa gccaagtagc gtttttgcga attgaccaga ctgcaccgtg
     5401 gctatttcag ccgatggatt atttagctgg tttaagttca ccattggcgt ggctagcaat
     5461 cggcgcaaca ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa cttcatggta
     5521 ttacagtcta gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc tagcagtttt
     5581 gacggcaacg aatattctac cagtcaattt tgaagcactg gcaacaatcg ttatcatgat
     5641 ggctacacca gctgcaacag tggcagcagc ctatgctatc agttttgata gggaggcact
     5701 tttagcctcc aatgcatcct tactgtcaac tatttttgct gtggtactga cacctgtatg
     5761 gatcgttgta ttacaaatga ttcatggcat aggtttattt tcataagaaa tggaggaaga
     5821 aagtatgaga atcgcatgtt acggtgttcg tccgaacgag atagattttt ttgaaaaatt
     5881 gaatatttac cattatgatc tcagtcttta tgaagaactg ttgacacatg acaatatcgt
     5941 gtcagcaaag gaccatgatg ctgtattgtt gaggggaaat tgtatagcag atgagaccaa
     6001 tcttgcaaaa atgcaggaat atgggattcg ttatgttttt acacggactg tgggagtcaa
     6061 tcatatcgat ctacaagcgg cagctgattt tggtatgaca gtagcccgtg taccatcgta
     6121 ttcgccaaat gcgatagcag agttatcttt gacttttgcc atgatgcttt tgagaaatac
     6181 tgcgtatacg acgatccgaa catctttcaa agattttcga gtggatgagc aaatgtttag
     6241 tcgtgaaatc agaaattgca cagtagggat cataggaaca ggcaggatag ggttgacgga
     6301 agctgttttg tttaagggac taggagccaa agtgatcggc tacgatattc atcagacaga
     6361 agctgctaaa aaagtattga cgttccactc gcttgaagag ttattagcca aaagcgatat
     6421 tgtcagtatt catgtacctc atatcccagg tgaaaacgat caaatgatca atgaaggatt
     6481 tttagctcaa atgaaaaaag gtagcatctt gattaataca gcccgtggag agcttcaaga
     6541 taatgaagct attttaagag ctttgaaaag caatcattta gctggttttg caaccgatgt
     6601 ttttgcaaat gaaacagagg tttttttccg atccttcaag ccatgggaga cgattccaga
     6661 ccctgctatc cagcaattag tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg
     6721 ttcaaataca gatgaggcat tatcaaatat gatttcgaca agtttcgaga actttcgtga
     6781 aataatagaa actggaaaaa cgaaaaatga ggtatctctt cctaaagccc gtcaattaag
     6841 ataagaaaac atggatattc gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt
     6901 tacttctgta tacttggact attttgtatg ggataattcg atgactcaac agtaatctgt
     6961 gagctttttc cgcccattct ttgtcaattc ataacaggtt agcggctggc cgatttccaa
     7021 atatttgatt ctttcgttca atatatcttg ttctactaaa aaattcaaat atttcttgac
     7081 ggatatacgt gacagtgagc ttttttgagc cagttctttt gttgaaaaaa tagtatcttc
     7141 ttttagaata aatgcactta ttttttttaa agtcagtttt gacagacctt taggcaaatc
     7201 gatgaatgat ggtatttcgt gaatgggagt ggtgtggttg aatacttgat cgatcaattt
     7261 ctgatcggct ttttcagtcg tttggattgt ctgagagaaa gcaagatact tttcgatcgc
     7321 cagctgaaaa cgttgcaaag tgaacggttt gatcaaataa tctaaggcac cgtagcggat
     7381 cacattcttt atggtttcga tatcatctgc agcagtgatc aaaatcactg ggatatcatt
     7441 tccttgctgt ctaagccaag ataaaaaatg gataccactt tcgccaggca tgtatacatc
     7501 aagcagcagc agatctgcgt ttttttgtct gaaatatttt tttccttctt ctacatttct
     7561 gaaactttcg attacgttca tattagggat gctttccaca aaagtacgat tcaattctgc
     7621 aaccattgga tcatcttcga ttatcaggac atttgtcatc attttgatct tccttttttg
     7681 gtaattctag atagacgatt gtaccgccat ttgtttgttt ggaaaattct aaaaatccac
     7741 cgtggcgttt gacgattgaa tggatgagat tcaatccaaa cccacgattg attcccttcg
     7801 tcgaaaaacc tagcgtaaat attttttctg agatttcttc tgatatcccg acaccgccat
     7861 cgcagacggt cacgatgaat acttcagctt ctgtgtcata gtttaaatga atccggacct
     7921 gtccgtttct ttcatgtttt tgtagcacgg cttcttttgc attatcaagt aagttcccta
     7981 acacttgcaa aagtgaatgg atggagtcat ccatctccat tttagggata aaagaatcgc
     8041 tttctaacag catttcaaca gattgttctt tggcttcgtt tattttgcct aataaaaaac
     8101 cggcaattgc aggtactttg atcagacttg agacttggcc aatctctttt ttataatttt
     8161 gatttagctg ctggataaaa tgggtgactt cttcatactt cttcaaatca atcaaaccag
     8221 aaatgacatg aagtttattc atgaattcat gggtttgagc tctcatggag tttatatatt
     8281 gtttcgtccc cccaagctcc tccattaatt tcattacttc tgatcgattt cgtaaagttg
     8341 cgactgctcc taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa atagtttcta
     8401 tcccatttaa atacatggat tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa
     8461 acaaaacgtc atatagatta tcactgattt ttttcctact tcgaaatcca atagctgctc
     8521 tgtttggtag aaaatgtttt ttgcaggtgt attgatcaag aggatagttt tatcagaaga
     8581 gatggctaag attccttcat ctacttcatt ttcgataatc gttttttcac ttaactgttc
     8641 tgctatttct tgtggttcaa gtccaagcag tacttttttc attttttggg caagaaaaac
     8701 ggcgcctatg atgcctacgc taagtccaat aatcaatgaa aaaacaatca ttttttgtcc
     8761 atctttgact tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg caccaataat
     8821 ctcttgatca taataaatag ggacgaaata gcggaagccg aaccctaaca ttccttcttt
     8881 ttcagagaaa aattcttgtc ctccaatcac ttttttcaca tcttcatgac ttgagaaagg
     8941 aagaccgatg taacttgggt ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac
     9001 gataaaatct aaattcgatg caatcatgat ttcatttgtt attccttgaa tcgcatgtgt
     9061 cgggtcacca gtctgcagct ttttttggat gagtttatca ttggcaacga ttttagcaat
     9121 gctagagatt ttatctgaca cactagatcg gttcatcgaa agaataaaat tcctgatcaa
     9181 tatggcagaa acaatcaaag aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt
     9241 ctggagcttt aatggttttt tcttaaaaaa aggaaacata ggctacctcg aaagaattta
     9301 ttgtgaaaat tttaacatga aaacaaaaat ttaacaaatt gtcaacgata aacacaacct
     9361 tcctataaaa aacaccactg agtcggtaaa agaagctata cagaattttc aaacattggt
     9421 atgatagacg tagaaatgga aggaggcgtg tatatgatag ggggaatcgc acttgagacg
     9481 atttattggt atacattgct gatttgtgca ggattggcag tcctattgat tatttttgga
     9541 gatgtattcg attttgatgg cccgcttgat ccgatgctta ttatcccttg gttaactttc
     9601 acaagcttat ttggttttct aggtgagcgt ttaacagatc aatcatcttt atttttattt
     9661 attgctagtg gaggcatatc gatagtgttg gtatttttgc ttaatttcta tatattgatg
     9721 ccgatgaaac atgccgaatc aacgatgtcg tcttctgaaa aaactctgga aggacaagta
     9781 gctgttgtga tcacaccgat ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt
     9841 accggttcca ttagtcgtcc agcttgtttt tacagccccc aagaacagac agctgagcgg
     9901 ggaacgaaag tattgatcat tgagatcaag gaaagaatct gttatgtcat gccttatgaa
     9961 agcatgataa aagcttaaag gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg
    10021 atcgcaatcg cagctttgat tttactgatg ttgctgatcg tctttgtagc gaagtaccag
    10081 acagcaaaac cggatgaagc gttgatcatc agtggaagtt atttaggaag caaaaatgtg
    10141 cacattgatg aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg
    10201 ccagtttttc aacgttcgaa tcggatcagt ctattatcga gtaaattgga cgtctccacc
    10261 cctgaagtgt ataccgaaca aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa
    10321 atcggttcat ctgtagaaga gattgctaca gcagcagaac agtttttagg caagacaaga
    10381 gaagaactgg aaaatgaagc aagagaagta ttagaaggac acttgcgctc gattcttggt
    10441 tctatgactg tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa
    10501 gtcgcttctg tagaccttgc aaaaatgggg ttgatcatcg tttctttcac tatcaaagag
    10561 gtcagagaca aaaacggtta tcttgactct ttagggaaac caagaatcgc ccaagtcaaa
    10621 agagatgcag atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa
    10681 gcagaaaaag aatcacaagc agctgaatta cagcgacaaa cagagatcgc agaatcatta
    10741 aaagaaaaag aactgaaatt agcaacctac aaacaagaac aagatgttgc taaagcaaaa
    10801 gcagaccaag cctacaattt agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa
    10861 atgcagatta aaatcgtaga acgccaaaaa caaatcgaac tggaagaaaa agaaatcaca
    10921 cgtcgtgaaa agcaatatga ttcggaagtg aagaaaaaag cagatgccga tcggtacgct
    10981 aaagaacaag aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga agcagaacga
    11041 ttccgtgtgg aagcactggc agaagcggaa gccaataaga cacgtttagc tggacaagca
    11101 gaagccgaag cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc
    11161 aatgccttca aggaatatgg cgaagcagct gtcttgagta tggttatcga tatgctaccg
    11221 caattgatgc gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat
    11281 acaggaagca gttcaggaga aaccagtgga gcgaaccgag tgacaaatta tgccacaaat
    11341 ttgctttctt ctactcaaga aacactaaaa gcgacaacag gacttgactt gaaggaaatc
    11401 gtagagaatt tgtcgacacg tgggactact agccaagtaa atatgtatga agcgccaaaa
    11461 gaggcagaac aaaactcgat gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa
    11521 aatggaggaa gactttttaa agttcttctc tccatttttt tattttattg caaaagaaaa
    11581 ttagtcctcg ctatttttct tttgtttttg gatcttagct tctatctctt gtaaatttgt
    11641 tcttagcaat cgttctttct tttggagttc gaccaaatct tctgatagtt cttccttctt
    11701 ttctgtttca ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg tccctataaa
    11761 agtatctagt acacgttgga tagcatacaa agcggattcg ccttgaggga tgctcaacgc
    11821 aatcagcaac aacgtagcta ttgcggaaat gataccggaa ttattgttaa taccatctga
    11881 cacgacgatc acaataataa ctagaatagg aagcaagatc aattctacca aaaaatcatt
    11941 gtgaaaatag cttttgacaa aaaaataaat gatagctaat gtaccaccta atgtattgcc
    12001 gatgatccta gactttccaa aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac
    12061 agcagataat gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa acaagataca
    12121 taaaaaaaca gctaaggcac ttttgatcgt acgcatacct agtcgaaaac ggccaaattg
    12181 catagagatt ctcctttttg gtctttttac tatcatacct aaacttttgt ctgatagcta
    12241 gtttttcatt attcaagtat aattaaaata aaaaaggggg aagtaaatgc gtataggaat
    12301 gcgaacagtc aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc
    12361 gctgttgtat tggccagctg cagggatcat tgcattatta agtgtaggaa ataccaagcg
    12421 ctctacattg cgtacgggca tgaatcgaat cgcagccttt atattagcta cggcaacagc
    12481 actgttcagt ttttttgctg ttgggtatac tatttttggc ttcagtcttt ttttattcct
    12541 gttcatcccg cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa attcggtttt
    12601 ggtcactcat tatttagtgg aggaaaacat gtcttggcag ctgatcggca atgaagcggg
    12661 attaatggcg attggattag tatttgcttt gcttgcaaat gtttacatgc cagatcgtac
    12721 gaagcagctg aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata ttttgagaca
    12781 aatggcagaa tttcttttat cagaaaataa agaagatgtg cagatcaaat gcgaacattt
    12841 actgacattc attcgcgaaa gccaagaaga tgccagagaa catcaagaaa actactggct
    12901 cagacagccg ctatactacg aaacttactt ctctatgaga agagcacagg ccaatgtgat
    12961 taaagacatg ctggaaaatc tagagagaat ccagcaacca gcttattatg gaaaacatat
    13021 ctatggttta ttgatttata ctgcagaaac attttcagag tcaaatgatg gcagacaaat
    13081 tttaacacgt attgaagaag tttatgtatt gtatcggcaa atgcctctgc cgactagtcg
    13141 ccctgaattc gaggatcgag cagaattgtt ccagtttttg cagtctttta aaagttttat
    13201 tgaaatcaag gcagaattct cccaacaaaa aatccaaaaa tagaggttgt gaattcagct
    13261 aatattcagg tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa
    13321 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgctc
    13381 cttgtgactc ggagctgaac ggtaggctca caacctcttt cttaaacggt gttcaaaagc
    13441 tttcctctgc ggtaataagt ccagccaaac gaaaaatagg aggagctatt tttgtttggc
    13501 tgttcttatt acttgatgca gaacagcttt ttcacaacct tttaaaatta catatttttc
    13561 catagaactt gcatttgatc caagatttcg atgacatctt ttgtcaagaa gagagagcga
    13621 ttgggcaagt caccttcaat catgtgtacc atattcttta tttcgtaatt cattgcttca
    13681 gatgtattcc cgctttcgat gaattccttt gtaccatctg taaataagat ctccgcacga
    13741 tcagcacgcg ggtaatcagc aatcgtcaca taaccgttct caaaggcaac gataccgact
    13801 tttggcattt ttgcctgaaa agtcaagctg acagcagcaa gttcattttc tttgttacgc
    13861 aagatagtga cggattgttc gtccacgcct gtttcaaaag gaaccattgt agaggcaact
    13921 acttcaggtt gagagctcaa aaagaaacga gcgaaagata cagcatacgt accgatatcc
    13981 aacagagctc ctccagctaa ttctggattg aagaaccggt ttttagggtc tggttctttg
    14041 taactgccga aaggtgcttg gatcattttt aaagccccca gttttcctgt atccattatt
    14101 gagcgtaact gctgataaag tggcatgttg aagattgtca tcgcttctgc taagatcaaa
    14161 ttcttttctt ctgctaagcg catagcgtca gccagttctt ttttattcat cgtgatggct
    14221 ttttcacata gaacgtgttt gcctgcttcc aatgctgcca agatatggtc gatatgctgt
    14281 ctatttggta cagcaatgta cacgatgtcg atttcagcat cgtctaacat ttcttgatag
    14341 cttccgtaag cttttggaat attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt
    14401 ctggaagcaa ctgctgttag ttcgctcgtc tcttgattga agctttcggc aaactcatgt
    14461 gcgatggtac caagaccaac gattccccat tgatattttt tcatctgaaa acgctccttc
    14521 atagttggat tgagtttctt gtctgtctat tattataaca aaaatcacga agaataaaga
    14581 ggagaggact tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc
    14641 aaaacttaag tagaatagtt aaaaggaggg gataaacgtg catcaagtat atattgatct
    14701 tattatgagt gtattattag aacagtttga aactgaaaaa aattttttag atgaatactt
    14761 gctgatcagt ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga
    14821 agcgatgcag aaagtaaaaa atcttttcac tgattatgaa tggatgctaa cgcaaaagat
    14881 tttgcgtcaa acgattattt ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca
    14941 aatcaaaaca cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat
    15001 ccctttgaaa aataatcagc aggctggtag ataccttgac ttgaaagtaa gtattcaata
    15061 tgatgaatgg ggctatgatg atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca
    15121 gatcgaagga gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg aagaaactta
    15181 cgttcatgag gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa gtgaataaat
    15241 ttttaggatt tggagcagtt gtgattatga tcgcagcaat gggctatgtt atttttagcg
    15301 atgatgattc gcatgttcaa gccggcaatc aagcagtcga acgaacaagc accaaaacaa
    15361 ctgctagtaa agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa
    15421 aagatcgtac gtccaaacaa ttgccagatg ttaaaacaga cgattggaag ctagttttgg
    15481 ttggtccaga tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa
    15541 gtaacggata cttgatcgac aaacgcattg ctccagaata tgaagcattt gccaaggctg
    15601 ctgaagcagc tggatttcca ttagtgatgg tctcagctta ccggtctgta tcatcccaac
    15661 aacaagtttt cgaacaaaat gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg
    15721 aggcgacaaa gattactaaa gaaacgatta cggagccagg atacagcgaa caccacacag
    15781 gtctggcagt cgatgtcgtc gatcaaaact ggtacaacag ttatccttct actgtattgg
    15841 atgcaagtta cggagatcag cctggagcta aatggatcgc agataatgct gcgcagtatg
    15901 gatttatcgt tcgttatcca aaaggacacg aagatataac taagatcaca tatgaacctt
    15961 ggcacttacg ttatgtcggt aaagaaaacg ctgagtatat caccaaacac caattgacgc
    16021 tagaagaata tctgaatgaa ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt
    16081 ggaaaaagca aaagcgcaag aatatccgtt ggccagcagt ggttgccggt gtagcagtga
    16141 tactcttagc ctttgttttt tccttgaaga atctgtcttc tccttttact gataatcagc
    16201 aagaaaatga agcgcaatcc catcagcaat ttattgaacg gcttgttccg catgcccaag
    16261 aactacaaga tggttatgga gtgcttccta gtatcatttt aggacaagct atattagaat
    16321 caaattgggg aaaaagtacc ttggctagca aatacaataa tcttttcggg atcaaggctt
    16381 atggggatca gaagaaagta agcctggaaa caaaggaatt tgttaacgaa gaatggataa
    16441 ctatccaagg agacttcaaa gtgtatgatt catgggaaca gtccatggat gaccatacac
    16501 aactgttcgt ccaaggagta gattggaatc ctgcgctgta tgaaaaagtg attacggcca
    16561 ccaactatca ggaagccgca caagcattgc aagatgcagg ttatgcgaca gaccccggtt
    16621 atgcgcaaaa aatcatccag gtcatcgaga cgtatcaatt gaatcagtat gaccggagat
    16681 agcaaaggag aagtggctga tggcagaaca atttacgcca gaaatcatga cggaattgag
    16741 aaaagatatc gtaagagtac cggaaatcat tttagaagca agcgggatca agatatttgg
    16801 aaaattgatc cgttcgatca tctttactac ggatattgcg atcatacgta atacaaatgc
    16861 gaatgcagta attgctgtgt atccttttac acctcatcca gcgattacta aaagcattat
    16921 tgaggcagct gacattccag ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg
    16981 ttcttcctac atgagcatgt tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg
    17041 tcctacgcct ttagagacag taaaagaagt ttgtcgagta gttgatattc cagttatcag
    17101 tacggtaacg tcgaaatata cagaaataga tgaaaagtta gcagctggta tttcgattat
    17161 caatatcagt gcaggtaaag aaactgcaga agcagtacgc cattttcgta aaaaatatcc
    17221 tgaacttcct atcattgcga caggtggtcc aacggaagaa agtattgctg agaccattca
    17281 agcaggagca aatgccatca catatacgcc accaagtagc ggagagcttt ttagtcgtaa
    17341 aatggaaaaa tatcgggaaa ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag
    17401 acgaggaaga gcagatagtg gctcttcctc gtcttttgta ctccagtact atttgttcag
    17461 aattcaaaaa aaagagaaat ttttttgttt ttttacctgt aatgtacata agaagagtta
    17521 aaaccttttg ttattattag cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt
    17581 ctatagaatg aaaaacgaat aaaaactaaa aaaggcaccc gacaatttag tagcagctaa
    17641 attgtcgggg cttgaaaagt cagcgggaac tgaccaatca agttgccata gtcaatgctt
    17701 gtgcatcgac gtgtatattg tacctaattt catacagaat tactagttat ttttctgttt
    17761 tttagggaag agcgcacacg aagaagcatg cttatttagc tatgtcaagg atattgggca
    17821 gttgtctgat atcctttttt ctttgcgtaa ttatcctccc atcattgaag caagaagtgt
    17881 ttttagcgat gaaagaagaa tcagcactat ctaatttttc ttgaaagtat tgagttgttc
    17941 atcctaggca gaacgataag gcaagtcttg gtttttattc cccttgaaaa agatcgttta
    18001 ataaatatgt tctgtataaa aatacagtat attgaaaaat aacaatggaa gtagaggtgc
    18061 agagaatgaa gaaaagaatg agaatcatct tagctttaag tgttatgtta ttcttcttga
    18121 cggcttgtgg gcaagaagat gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc
    18181 aagtccgaca cgatgcaaca gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg
    18241 tgacgatcga ttatgaattt tatgattatg aaggatatta cacaaaatta ggtacactag
    18301 cagcatcaga tgatatctgg gacattttcc aactgaacaa tcaatttcct caatacatca
    18361 atcagattga accattagat gaatatgtgg agcaagggat aattgatatt tctaatgcag
    18421 atgaatccta tttagcaacg acgacttacg acgggcaatt agttggactg tctaacgggg
    18481 tgaattcgtt tgcgattgct tacaataaaa atatatttga acaattaaat atccctgaac
    18541 cagcttcaaa ttggacatgg gatgaatttg aacaaatcag ctatcaaatc actgaagaat
    18601 taggaatcta tgctatttca agattcgaag attttatagc tggctgtatt attcaaatac
    18661 cacaagttga gaaaggattg aatttttata atcgggaaga tctatctaac agcttgggat
    18721 ttgaaaatcc agactatcta acagattttt tccagctgcg taaaaatctg gtagacaaag
    18781 gggcccatcc agaaccaggt ggtggagctg caagtacgac ggatctgtct tttcaggcgg
    18841 ttagggatag agaagcggca atggtgtttc tatcaagtaa ccagttgaca gaaatattgt
    18901 caggggctcc tgaagatatg gagattcagt tgattaatcc acctcgcaga aaagcagatg
    18961 gagaatctgg tatcccttta cgttcttcgc aaatgctgag tatggcaaaa agctcagccc
    19021 ataaagaaga agctgctaag tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa
    19081 ttttaaaagg agaacgtggt gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg
    19141 cagatgatgc aactttgcaa gcgacatttg attatttaga aatgatttcc gatatggaca
    19201 acggcgaagt gaatgtcttt gatagtgctt tgaatccgga aattcaagac caatacaatc
    19261 ttttagttga aaaagtcatt tatgaagaag taacagcaga aggaaggcat gaaaaaagaa
    19321 aaactaaaac ggttcttgga tcaagacaat gtggtcggtt acgtatttgc ttctccattc
    19381 atcctaggat ttctattgat aacagtgata ccgatgatct tgtcacttta ttattcgttc
    19441 accgattatc gtttgggaaa tccagtctca tggatcggct tggataatta tataaagatg
    19501 ctgagtgatg cccgtttttt gaactccatc cgagtcactt ttctctatgt atttgcatct
    19561 gtacctgtga aactgatctt tggtctattg attgcttatc ttttaacgag gaatatcaaa
    19621 ggaacgactt tctttcgttc tttgtattat gtgcctagtt tgatcggtgg cagtatttct
    19681 atcgctctag tttggcggga gttgttttct aaaaacgggt tagtgaatat tttcctggaa
    19741 cgattaggcg cacctgcgct ttcctggttt agcgatcaaa aactggtcat gatccctctt
    19801 gttttgatgt ctgcttggca attcggttcg gcaatgatca tctttgctgc aggtatcaaa
    19861 gagatcccag gctcgtatta tgaagcggca gagatcgatg gagcgaacaa atggcagacg
    19921 ttttacaaga tcacgttacc tcttctttcg ccgatcattt tgtataactt ggttatgcag
    19981 acaatcgcgg cgtttatgac ttttacgcag gcatttatca ttacccaagg tggtccgaat
    20041 gatgccacga acttttatgc tctttatgtg tataaccaag ctttttcttt taattctatg
    20101 gggtatgcta gtgctatttc gtggatcatg ctgttgatta tgggagctgt cacctttctg
    20161 attttcaaat catcaaaaaa atgggtcgtt gtgtgaatga gaggaggaag aaatgaataa
    20221 tacagtaacg aaatggtttc aatacggttt tattttgcta ttaggtctag tcatgattta
    20281 tccggtctta tggatgatcg caggttcttt taaaacggat caagagatta tgagtggttc
    20341 gctgaatttg attccagaga cttttcaatg ggaaaattac gcaaaaggat gggcggggtt
    20401 tgctggaatt tcatttttta ctttctttag gaattcgttt gtcatctctg cagtttcgac
    20461 gatagggaca gttctgagct cgacttgtat tgcttacgca ttatcgagaa tcgatttcaa
    20521 aggaaaaaga ttctggttcg tcgtcatgtt agtaacgatg atgattcctg cacaagtgat
    20581 tttgattccg cagtttatta tttacaatcg gctgaatttg gttgggacgt atgtgccgct
    20641 gattttacca catttttttg gacaagcatt tttcatttac cagatcatgc aattcatggt
    20701 cagcatcccc aaagagttag atgaatcagc gattattgac ggatgcagca agtatagtgt
    20761 ctttaccaaa attatttttc ctttgctgaa accttcgatc atcacgacga tcatcatcca
    20821 gttttattgg aaatgggacg acttcatggg accattgatt tatttaaaca agccgagatc
    20881 ttacacggta tcgattgcca tcaaattatt tgcagacgct ggatctagta cgacttacgc
    20941 gagtatgttt gcgatgtcta cgttgtcgct gctaccggtt tttctgattt tcttattctt
    21001 caataaatat cttgtccagg ggatcagtac aagcggtctg aagggctgaa tatgaaaaag
    21061 ccaatagaag agatgtttct ttacaagctc aactactatg tctgtcggat ttttttaacc
    21121 agtcttatca gtaatgctct tttcgcttta gttctaggat gtttgttcac tttaggatta
    21181 gtgctgtctg ctcgtgttgt ctatctgatt tattttttga tgatgtatcc ttcttattat
    21241 ctatctgtcc attacttggc agaaaaaaag gtatgttgga ggaaagggaa aatcaaacgg
    21301 atgtgtttca tcattacgtt gtttcagctg tttgtcatta tttttgcagt tgattggtat
    21361 ttattagtgg gaaagattgg agcttggtat ctgattcctt ttctattgtg tctgttgctt
    21421 tttgtatccc atagtttttt tagtacgcta attgatatct gcaaagaaga taaagcagat
    21481 agtcttgttt atttgatcaa aaggaatatc tggctgttgt caaaaaacgt caaggggttt
    21541 ctgtggtatt tatgcctttt gtatggactg ttctggtgta tacagcctga ttttttactc
    21601 actgcattac tgtatccagg gctgccattt gtcacagtat tttgcttaaa agaaaaggag
    21661 aatgacttta aatgaagaaa aaatatgtac aagtcggact tggtgggaga gcgcgttttt
    21721 tttatgaggc aattgcctct acgtatcatc aaacttgtga actagtcggt ttttgtgatg
    21781 tatctcaaac aaggatggat tatgcaaatc ggatacttgt gaacaagtat ggacagaaaa
    21841 aagtaaagac atgtatgcca tcacaatttg agcaactact ggatgacgta cggccggctg
    21901 aagtcattat tacgtcagta gatcgaacgc atcatgatta tatcattcgt gcaatgaaaa
    21961 aaggatatga tgttatttgc gaaaaacctc tgactatcga tgaggaaaaa gctcaagaaa
    22021 taataacggt ccaaaaacag acgggaagaa gcctgcgagt cacttttaat tatcgttatg
    22081 caccgtatca cacgaagata aaggaactga tagaaagtga tgtgattggt gatgtgtttt
    22141 ctatccactt cgaatggctg ttaaatacgc aacacggggc agattatttc agaagatggc
    22201 atcgaaataa gcacaatagc ggcgggcttc ttgttcataa atctacccat cattttgatc
    22261 tagtgaattt ttggttgaac agccgtcccc aaaaggtatt tgcattcggt ggattaaact
    22321 tttatgggat gcataatgcg gagaaacgcg gagtagaaat attttacagt cgatgctatg
    22381 gaagcgaact agctaaagct gatccatttg ctatagacct gtcagccaat gaggaattgc
    22441 gagaaatgta tttagaagca gaaaaagaca gtggatatat tcgtgaccgc agtgtatttt
    22501 ccgatgatat ctctattgaa gatacactag gcgtaacggt caaatatgaa actggtgcga
    22561 ttttgacgta ttcgctaaac gcgtatatgc cgtgggaagg attcaatgtt tctttcaatg
    22621 gcagcaaagg acggatcgaa atgaaagtga tcgaaaagtc ttacatcaat gcaggtggag
    22681 ataaaaagga tgaaggagca gccgttcttc agtccatcca tgtctatccg atgttcggtg
    22741 agccatatga ggtaatcgtc gaacaagaag cgggcggaca tggcggagga gatccgcgtt
    22801 tattagatga tctatttggt gaagaaaaag aagaagatcc gtggaatcgg gcagcaactc
    22861 acgtagatgg tattttgtcg attttgacag gaatcgcagc aaatcattct attgcttcag
    22921 gaaaagcagt agtgattgac gagcttgtca gcttaaaaga ataagaatag atttacggaa
    22981 attacttccc gtagtttata aaatcttgcc ttattgccga taggctgtct tttgaacact
    23041 atttattcgg aaaaaggggc tatgacatgc tttttgtcat gccttctttt tatgtgggct
    23101 gcttttttag ataggagaaa ataaaaataa ctatttaaac ccgaacaaaa aggaataatg
    23161 cattaaaaat cattagattt tacaaaaaaa taagtaaacc acgttgtcag tttcagaaag
    23221 tagtgttaga ctgttttcaa tatttgaaac aacatttttt attcaaatat ggcaaactgc
    23281 cttcacgtgc aaaacagttg caagaaaatg aatttacact attattagtt ttacatacct
    23341 acttatatat cgtcataata tggatgacag tttctagcca gtaccgtaaa tgctggacta
    23401 taagtaaaag attggctatt tcacccttct tttcttatga taaagtggag ggtgtttttc
    23461 tttaacagaa acatagtagg tttttgcaca aaagcggtta tttcggtatt aataaatgat
    23521 aaaggagcaa ataaagtgaa agaattagtt gaacgtattc aaaaagacgg acgtatatta
    23581 ggcgagggcg tattaaaagt cgacagcttc gtcactcacc aagtagaccc tgtattgatg
    23641 gaacaaatgg gaaaacggtt tgcagaagta tttaaagaac aaggaatcac gaaagttgtg
    23701 accattgaag cttctgggat cgcaccagct ttatttgctg caagagaatt agatgttcca
    23761 atgatcttcg ctagaaaagc aaaaagtttg acgatggatg aagaattatt gacagcttct
    23821 gtttattctt ttacgaaaca agtgacaagt acgatttcta tttctcgtaa atttttatct
    23881 tcagaagaca aagtattgat tatcgacgat ttcttggcaa acggacaagc ggcaaaagga
    23941 ttgatcgaat tatgccagca agctggagcg caagtagaag gaatcgggat cgtcatcgaa
    24001 aagtctttcc aagatggacg tcagttactt gaagaaatgg gattgagagt cgtttctctt
    24061 gcgcgtattg cttccttgac gaatggacaa gttgagtttc ttgaggagga cgcataatcg
    24121 tggaaaaaga aacacaaaat ggaaaagctg cggttttagg gctgcagcat ctgctagcga
    24181 tgtatgcagg agcagtagcg gtgcctcttt tgatcggtac aggactaggc tttaatcaag
    24241 aacagatgac ttatttgatt tcaattgata tctttatgtg tggattagcg acattattgc
    24301 aattgacagt taaccgattt ttcggaattg gcttgcctgt cgttttagga tgtgcgatcc
    24361 aagcagtagc tccactgatc ttgatcggaa ccaatgaagg agtaggagct atctacggat
    24421 ctattatcgc ttctgggatc tttgttgtgt tagtttctgg tgtcttttct aaaattaaaa
    24481 aactattccc tcctatagta acaggaacgg ttatcaccgt tattggtttg actttgattc
    24541 ctgtagcaat cgagaaaatg ggtggaggta atgcaacagc agctgatttt ggtgacaaaa
    24601 caaatctgct acttgcattt gtgacgattc tcctaattgt aggcgtgcaa atgctggcaa
    24661 aagggttcat tcgttcaatc gcggtattga ttggtttagt cggaggaagc atcttagctg
    24721 ctttcatggg aatgattgat ctgagcgcga ttggagatgc gccgttgttc catgttccac
    24781 aaccctttta ttttggtaaa ccaactttcg acgtatggtc gattttacta atgatcatca
    24841 tttccatggt cagcatggta gaatcaacag gtgtatattt tgcacttggt gacattactg
    24901 ggaaaaatgt tggagaagaa gaattgaaaa agggatatcg tgcagaagga cttgccgtta
    24961 tcttaggcgg tattttcaat acattccctt atactggctt ttctcaaaat gtcggattgg
    25021 ttcaactttc gggaatcaaa acaagaaaac ccatctattt ctctgcattc ttcttgattg
    25081 ttttaggatt attccctaaa atcggtgcgt tagcacaaat catccctgag ccggtacttg
    25141 gtggaggaat gttagtcatg ttcgggatgg tggcagtcca aggaatccgt atgctgttgg
    25201 aagttgattt cagcaatgat aaaaatctat tgattgttgc tgtttctgtt ggatttgggc
    25261 tgggtttcaa tgttacgccg acattattca gtcaaatgcc gcaaacattg caaatgttca
    25321 ctggaaatgg catcgtaatg agtagcatta cggctatcat attgaatttg atctttaatg
    25381 gattgaaaaa agatgaagaa atattggaag aagccttcaa gaagtaatag agcaaaagaa
    25441 aggttgtgaa atcagcgttt tgacctgatt gttagagaaa caaacggaaa aatagttcct
    25501 cgtattttgc cgtttgtttg agctaatacc gagggtcagc tgattgaaca ccgtttatcc
    25561 ataaaaagga atgtgaccta acttttgtca cattcctttt tttcatcttt aataaacacg
    25621 aacaataaaa tgtttttctt tgttaacatt cgaaatttct ttgacatcaa aaaaagtgat
    25681 tgttaaaata tgaatgtaga aaaactggga ggaaaaacaa tgacagcaaa aatctctgtg
    25741 attatgggga gtacttcaga ttgggaaaca atgaaagaag catgtcaaat attggatgaa
    25801 ttcggtgtgt cttatgaaaa aaaggtcgtt tctgctcatc gaaccccaga tttgatgttt
    25861 gaatatgcac aaaacgctcg aaaaaaagga atcaaaatca ttattgcagg agcaggggga
    25921 gccgcgcatc tgccgggtat ggtcgcatca aaaacaactt tgccggttat cggtgtaccg
    25981 gtccaatcac gaacattaaa tggtttggat tcgcttcttt cgattgtcca aatgcctgga
    26041 ggagtacctg tagctactac agcaatcgga aaagcgggag caattaatgc tggactactt
    26101 gcgatacaga tgctttccat gtatgattta gaattggagg ctaaattggc tgaaagacgt
    26161 tcaatgttgg ctaaaactgt tatagaaagt agtgatcaac ttggttaaac ctttgttgcc
    26221 tggttcaacg atcgggatcg tcggtggcgg tcaattaggt cgtatgatgg cgattagtgc
    26281 aaaagaaatg ggctttaaag taggtgtgct ggatcctgtg agcgattgtc cagccgcaca
    26341 agttgcagat tggcacattg aaagtgtcta tgatgacacc tttgcgctga aagagcttgc
    26401 cagaagaaca gatgtcatta cctatgaatt cgaaaacgtc agtgtagagg cgttaaatgc
    26461 gattttaccc atgtcattta ttcctcaagg gacagacttg cttgcgatta cgcaagaccg
    26521 cctgttagaa aaatcttttt tggaaacgaa taacatcgtg attgctcctt atgcaacgat
    26581 cgtcagtccg acggatatcc aagatgcgat tgacgggatc ggctatcctt gtgttttgaa
    26641 aaccactcga ggcggctatg atggcaaggg gcagtatgtt ttgaaaagtc gggcggatct
    26701 agctcctgcc atggaccttt taagagaagg aacctgtgtt ttagaagcat ggatcccgtt
    26761 tgaaaaagag atttcgatca tggtggcagg aaatggtcaa ggagatttca cgacttttcc
    26821 ggtcgtggaa aacatccacc acaacaatat tcttcatgaa acgattgctc cagccgcgat
    26881 tgatcaggat gtgatcgagg aggctgagcg tattgcccgt gtgattgcag aagcagtgag
    26941 cctttcaggt gttttagggg tagaaatgtt tttaacaaaa acaggcgggc tatatgtgaa
    27001 tgaattagct cctcgtcctc acaattctgg acattattct attgaagctt gttctatgag
    27061 tcagttcgat gcccatattc gaggtatttg tggttggcca ttaggagaaa aagtacgttt
    27121 gataagcaat gcagtaatgg tcaatgtttt gggagaacaa ctgctggaaa gctatcgctt
    27181 gattgagaat aagccagact ggcaattcca tttctatgga aaaaaacaag caaaaaaagg
    27241 tcgcaagatg ggacatatca ctatcttgac agaagacatc cagcaaacgc tggaagaaat
    27301 caaacaaacg aatatttggg attagaggag aaaaaacatg ttagaacgtt atacacgccc
    27361 tgaaatgggg aatatctgga cggaagagaa caaataccgt gcgtggctcg aagtcgagat
    27421 tttagcagat gaagcttggg cagaacttgg tgaaatcccg caagaagatg tgaaaaaaat
    27481 tcgtgagcat gcatcatttg atatcgatcg gatcttggaa atcgaaaaat taacacgcca
    27541 tgatgtcgtt gcgtttacac gagcggtttc tgaaacattg ggagaagaaa gaaaatgggt
    27601 ccattatggt ctaacaagta cggatgtcgt agatactgct tatggctatc tcatcaagca
    27661 agcaaatgat attttacggg aagacttgaa acgttttacg gaaatcattg gcgaaaaagc
    27721 aaaagaacac aaatatacag tgatgatggg tcggacacac ggtgtccatg ctgaaccaac
    27781 tacttttgga ttgaaacttg ccacatggta ttcagaaatg aaacggaaca tcgaacgatt
    27841 tgaacatgcg gcaaaaggag tagaagcagg aaaaatctca ggagcagtgg gaacgttcgc
    27901 aaatatcccg ccatttattg aagagtatgt atgtaaacaa ttggggatcc gtccacaaga
    27961 aatctctact caagttctcc ctcgggatct acatgctgaa tatttctcag cgatggcgct
    28021 tattgctaca agtattgaac gttttgcaac agagatacgc ggattgcaaa aatccgaaac
    28081 acgtgaagta gaagaatttt ttgcgaaagg tcaaaaaggt tcttctgcta tgccgcacaa
    28141 aaggaatccg atcggttcag aaaatatggc tggtctagcc agagtggtgc gcggacacat
    28201 ggtcactgcc tatgaaaatg tcacattatg gcatgaaaga gatatttctc attcttcagc
    28261 agaacgaatc atcttgcctg ataccacgat tttattgaat tacatgttaa atcgttttgg
    28321 caatatcgtt aaaaatctaa cggtctttcc tgaaaacatg aagcggaata tgtctgcaac
    28381 atttggtttg atttatagcc agcgcgttat gctgaaattg atcgataagg gaatgacaag
    28441 agaagaagct tatgatcttg ttcagccaaa aactgcgtac gcttgggaca accaagtgca
    28501 gttccgcccg ctgttagaag aagatccaac catcacatcg gtattatcta aagaagaaat
    28561 agacgatgcc tttgattatc atcatcattt gaaaaacgtt gacgtgatat tcaaacgagt
    28621 aggtttggaa taaagaagag tgagttgcaa aattcaatta aaatagaata gcaaaaataa
    28681 ccaaaaaaga agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat
    28741 caataatagg aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa
    28801 aaaatagagg aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag
    28861 atgttgcagc aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg
    28921 gatacgataa tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc
    28981 tttgtgttct gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc
    29041 tcggcggatt tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt
    29101 gatcgacagc aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa
    29161 aagctgacct tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca
    29221 aatttttagt taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt
    29281 ataagatttt cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac
    29341 atatttttgt tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa
    29401 aaacgatgta tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa
    29461 cttttgtatg aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg
    29521 atagaatatt tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga
    29581 aaaggagaat tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga
    29641 ataaaaaatc attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa
    29701 attatccctc tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta
    29761 ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac
    29821 cagttggatg atccatttat caatgaagag catatctatt tcttacaact tgcaaaaccg
    29881 gaagaaatca gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta
    29941 tttgctaagg tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga
    30001 caaattttgc ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg
    30061 aatgaacata tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat
    30121 caagaagtat ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt
    30181 tcgtaaaagt atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa
    30241 aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact
    30301 ttgagataca acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg
    30361 acaagctgct tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg
    30421 aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg
    30481 ttatgggcag ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc
    30541 tctttagaag gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg
    30601 agatgcggtg cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa
    30661 gaagggaaac cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt
    30721 ttgccaggtg tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg
    30781 aaagtcaacc ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt
    30841 ccagttgctc atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa
    30901 aaaaataatc agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt
    30961 gcagggatta cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca
    31021 gtagaagact tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac
    31081 ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag
    31141 aaaaaaagaa tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa
    31201 gatatcttag gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc
    31261 gaacattgtt cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct
    31321 catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca
    31381 gttgtcttca aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct
    31441 gcgacaggag ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg
    31501 ttgctagatt cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa
    31561 gaagtagttg caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc
    31621 gaaattgcat ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt
    31681 ttgattgaac acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg
    31741 tatgtaggtg caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa
    31801 tttaatcaag aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa
    31861 aagctgctgc ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt
    31921 atacaggata tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct
    31981 ggctcaggac ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct
    32041 tatgaaatga tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag
    32101 gaagaaaaaa tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa
    32161 gtaacagacg acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca
    32221 gtcgatgcat tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga
    32281 atcgtagaat ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta
    32341 ttgcttgatc tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat
    32401 gattcacaag tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg
    32461 gttagaggga cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat
    32521 ttggatccaa aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct
    32581 agcggtggta agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca
    32641 gaagtttttt gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta
    32701 ggaactcctg ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc
    32761 tatccaacac caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact
    32821 caggaattca aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt
    32881 gatggcagtg aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt
    32941 gatctatcca ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg
    33001 attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct
    33061 ttcaaacatc agctagggat atctgtacag tttgaattgt cctctgctca attatttgca
    33121 gaaactcaat caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa
    33181 atgatgggag atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt
    33241 tcagcaactg acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat
    33301 gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt
    33361 ggggtcatcc agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag
    33421 gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt
    33481 tggggctgct ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag
    33541 ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc
    33601 cttttctatt caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca
    33661 atgctcgttc ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg
    33721 atacggagat attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat
    33781 taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg
    33841 caatgattgc cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa
    33901 acggagcata cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta
    33961 taagagatgt ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt
    34021 gctttacgaa ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac
    34081 cagattcaaa catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt
    34141 tagcgaaaga atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt
    34201 cagccgcaag cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa
    34261 atcaatacat tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc
    34321 gaatgaaatt atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg
    34381 attcaattgt tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg
    34441 caaaagaagt ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga
    34501 ttgatattca aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag
    34561 aatgtatcgg tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg
    34621 gtctggaaaa agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc
    34681 cgacgccgtt atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt
    34741 tcttttaaaa gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa
    34801 gctggcgtag atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa
    34861 aaaacggaac gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta
    34921 tcggtctttc aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa
    34981 ttagcagtgg caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg
    35041 tgtgtcaatg atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca
    35101 acggggaaaa atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt
    35161 gttcaaggaa atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca
    35221 ggtaatgatt atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg
    35281 acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat
    35341 tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca
    35401 tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc
    35461 tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc
    35521 actggtggag ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa
    35581 atcaaacttg gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa
    35641 attcctgaga tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc
    35701 gaagaatcga aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat
    35761 gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga
    35821 tgagaatcgc tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc
    35881 tatcgaaaaa aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat
    35941 atgttttgaa acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat
    36001 ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg
    36061 atttgatcgt attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg
    36121 ataaaagaat catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca
    36181 tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc
    36241 aaggtgtaga tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca
    36301 cgttcgattc tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga
    36361 ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc
    36421 agataaaact gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat
    36481 ttcaacagga ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga
    36541 agaagtgaca gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat
    36601 ccatggtggt cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga
    36661 aaatatccaa ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat
    36721 gaaaccagat gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat
    36781 gctccgtagt gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta
    36841 tgaagaagta ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa
    36901 actagcggca aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt
    36961 aacggattgg gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca
    37021 aacgcttcgt tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct
    37081 ggaaaaatat tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa
    37141 tatccgagat gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt
    37201 cgccttgaaa cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg
    37261 ggaagcagcg tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg
    37321 agaagtagat ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc
    37381 cccttcttat acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact
    37441 gacagtagac tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg
    37501 tggacttctt gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt
    37561 gacaaaacgt aagccgactc cagcagaagc aaaagcatta gcgtttgctt ggagagctgt
    37621 caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg
    37681 agccgggcag atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa
    37741 aatggaaggt gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata
    37801 tgcagccaaa catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga
    37861 ttctattgat atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt
    37921 tagacattag agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga
    37981 acatgcaata agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc
    38041 aggaaatccg gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca
    38101 ttcagcgtta atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga
    38161 agttcctttg ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg
    38221 tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga
    38281 tacttatcaa atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc
    38341 gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa
    38401 aggtgtagtc gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa
    38461 ccagcatcga tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga
    38521 agaattctcc cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca
    38581 agatcataag cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta
    38641 ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa
    38701 accagcggct ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg
    38761 tttgattgct acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc
    38821 tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt
    38881 attagagaat cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt
    38941 ggttgcagct gccggttacc ccgaagaata tgagacagga atggttctac cagattttct
    39001 ggaagaagag ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg
    39061 agcaggagga cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa
    39121 aaaaatttac accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat
    39181 tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata
    39241 aaaaatacaa caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg
    39301 tggtacactt ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag
    39361 gtacttctga tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg
    39421 aagatccagt aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg
    39481 cagatattta tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat
    39541 caatcaaacg agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt
    39601 ttgatttttc taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa
    39661 tggattatgt ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag
    39721 tagctgaagc gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca
    39781 atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta
    39841 atcagctgca atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata
    39901 tgacagatga agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg
    39961 atatgacgat ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg
    40021 ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa
    40081 cgacaccaac tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga
    40141 tcgccggtac gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata
    40201 tgtctcggga agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat
    40261 tgtcataagc aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt
    40321 tcagcttcga gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat
    40381 ttcgtttatt agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa
    40441 atcccttgtt ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca
    40501 aaagcaatat ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt
    40561 ctttgttctg tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg
    40621 aaatgagagg gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg
    40681 gtaaaatttt ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt
    40741 gaaggctgtg agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa
    40801 agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct
    40861 tgcgggattt tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa
    40921 cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta
    40981 gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa
    41041 tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg
    41101 gaactggcgg tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt
    41161 ctttctgcga caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca
    41221 tggaccaacg tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca
    41281 actattaaaa atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc
    41341 ctaaataaaa acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact
    41401 tggtttgctg ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca
    41461 gcaagtttca atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga
    41521 ttttttttca attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct
    41581 aaagcagtta ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc
    41641 agataatcat ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca
    41701 ctaataaata tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac
    41761 ccgtccatga aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt
    41821 gaagacagtg ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa
    41881 ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata
    41941 aaaactttat acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt
    42001 gacagttgtt ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta
    42061 ataaagaagg agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc
    42121 gatccctttg gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc
    42181 gttgtctata actttgaatt ggatcatctc atcaattttg catgcggtaa tacgaataaa
    42241 tcctttgtga cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa
    42301 gatcattttt aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag
    42361 catatggccg tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt
    42421 ttcaagagga atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc
    42481 atgggtcata gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc
    42541 caaactgtta taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg
    42601 tgctaaatgg tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat
    42661 tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa
    42721 gccaaggttc cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg
    42781 gacctttctc tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac
    42841 tccaagttcc actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct
    42901 tgctaagtcg atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac
    42961 agaatctacc tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt
    43021 attgatcagc tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa
    43081 cgtactaatt tctttaagtg tatttgtatt atttttgatc tctcgaatat cattcctaag
    43141 ttcttctagg atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc
    43201 ccacatactt tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat
    43261 atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag
    43321 caaagggata acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat
    43381 gcttaaattt tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta
    43441 tattctgctg ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg
    43501 tttgaatatt tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt
    43561 tacactcgac tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc
    43621 catcaatagt tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag
    43681 ctaaagcacc gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat
    43741 catttgtttc taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga
    43801 caattcggtg aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg
    43861 ttggtttacg attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt
    43921 tctctgaacc agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat
    43981 tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga
    44041 cgaaaagctc gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg
    44101 ctcgctttgc ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga
    44161 tcatatccac tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca
    44221 tgtagccatt tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt
    44281 cagtatgctt tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg
    44341 ggtcaggtgc ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa
    44401 gggctaaaaa aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact
    44461 atcttatcag ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga
    44521 aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa
    44581 aaaaatcaat gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg
    44641 ataaaaaatg aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag
    44701 cattctatta agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg
    44761 aatcgctatg ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc
    44821 ggaattagaa aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa
    44881 tcaagtagca caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc
    44941 tatcgatggt tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt
    45001 gatcgcctat gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga
    45061 taattttgga gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga
    45121 aggaaaagga ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact
    45181 aatcaattac aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt
    45241 cgtgccatct ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac
    45301 ggcgcaagca aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc
    45361 tgttttgtcg ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg
    45421 gtatgggagt gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg
    45481 cgttaaatcg atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt
    45541 agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa
    45601 tgatactgaa acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt
    45661 atctgtcgat aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc
    45721 cgatctgaaa aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg
    45781 ataagaatta tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga
    45841 atccgagcgt tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc
    45901 ggagaaaacg gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat
    45961 ggctcctatg acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa
    46021 gatagtgaag aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt
    46081 tcaataaaag agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg
    46141 gaactgacag aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg
    46201 aatcttctag tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca
    46261 ttatcaaaat ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa
    46321 gaaagcgcga atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc
    46381 attatttcac ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg
    46441 gatggacaga cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc
    46501 gggatggtgg gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa
    46561 gtttatttta aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata
    46621 aacgaccaca tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg
    46681 ggagcgggca gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata
    46741 tccggacgta tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt
    46801 gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg
    46861 gatattcggg aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta
    46921 gataaagaaa aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca
    46981 aactccatcc atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta
    47041 tccaagtggc tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata
    47101 gatgttgggg caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa
    47161 gctgtctgtc ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat
    47221 acgatgaacg aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc
    47281 ttgatgaaat taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac
    47341 ggataaaaaa gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta
    47401 cagcatggtg atcattttag cgggactact tattgcgttt caattgatga cggatggtat
    47461 tttctggcgt ccattgaata taactaacct tgttctgcaa aatagccata tattagtact
    47521 agctgcaggg atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt
    47581 gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg
    47641 gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg
    47701 ggtagcttat gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg
    47761 tggactgaca caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca
    47821 aaaaatgtct acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac
    47881 attgatttta ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa
    47941 tcgtcggaaa aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt
    48001 atttacagca gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc
    48061 gattattttg atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac
    48121 agtagcaggt cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg
    48181 gatcaaaaca aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt
    48241 agctggatta gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat
    48301 ggaactggat gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg
    48361 aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc
    48421 gattttaggc gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc
    48481 cgttgtgcta gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc
    48541 agaaaaaaga ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc
    48601 attgtttcgt ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct
    48661 ctttttgctc ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc
    48721 cttgattaaa ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa
    48781 tgaaagtatt tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc
    48841 gttctgaatg tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc
    48901 ctgccaacat gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt
    48961 atttctatat catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga
    49021 aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta
    49081 tagagaagtt ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg
    49141 gacatgctaa ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt
    49201 ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg
    49261 cagatgcaac aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag
    49321 gctttgggac tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca
    49381 agccgatcat cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt
    49441 ttggtgcgac aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg
    49501 aaacaaaagt cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc
    49561 aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt
    49621 tgaaagatac actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag
    49681 gaagagatct ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta
    49741 atggagacac aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc
    49801 attaagtaat aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat
    49861 tattatcgca gatacaagct tttgaacatc attattcaga ataagggact gtgataagat
    49921 ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt
    49981 aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa
    50041 aaaacaagga ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg
    50101 aaggatcaat cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt
    50161 gaatcgttcc accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag
    50221 caaacgtggc agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag
    50281 aatcgattgg cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa
    50341 aataagagaa gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat
    50401 gccagatgag cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc
    50461 ggaagaaaaa gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta
    50521 tttggaaagt aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg
    50581 tcagcaagca atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt
    50641 agagtcacct tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt
    50701 tgggataccg atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa
    50761 aaataaattg aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat
    50821 gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat
    50881 tgaagacgat gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg
    50941 aacgattgat ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac
    51001 aacgaaaatc ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca
    51061 agaaatgatg gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa
    51121 tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact
    51181 gataagcgct tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta
    51241 ctatgcctgg attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt
    51301 agatatgggt cttggaattg caccaagtct actttttggg caagatataa atggaatcag
    51361 aatcaattac agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat
    51421 tctagaaaag tggttaacag aataatttca caaagtaaat agattaaaga atataacagc
    51481 ttggatttaa atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac
    51541 taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca
    51601 acaaaactgt ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga
    51661 aaatagaaaa aatgcagatg aaaaataaaa cgctttcctt attgtgattt tattcactaa
    51721 tgctgttaat atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct
    51781 tgtatttctc ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt
    51841 ttattcactg tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt
    51901 gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg
    51961 cagatttccc agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag
    52021 tacctgattt atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg
    52081 tattagacca acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa
    52141 ctgctggaca agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc
    52201 ttcttccagg ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag
    52261 cttttttatg gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc
    52321 taccaccaca aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt
    52381 tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac
    52441 aaggtgattt ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt
    52501 atatccaaaa caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa
    52561 cacttgcaca aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc
    52621 cgcttgcagt atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac
    52681 cagtcctaat cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc
    52741 caacccgcta ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc
    52801 gcttccgtaa atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca
    52861 tcgaagcaac taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac
    52921 aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac
    52981 aaattgcaat ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca
    53041 agcaatcaca gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg
    53101 tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt
    53161 cggagaagac cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt
    53221 cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt
    53281 cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac
    53341 tagaaatcta cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt
    53401 gcattcggat aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc
    53461 ttccaaccct tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt
    53521 tgttttccta gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc
    53581 ttacgaagtt cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat
    53641 cacatacgga gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa
    53701 tatatctgtt gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat
    53761 tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg
    53821 cgttgccgcg caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc
    53881 aatcggacga gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg
    53941 gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa
    54001 gaagggacga aacaaagtca tggcttatca atttaaattg ccggatatcg gtgaaggaat
    54061 cgcagaaggt gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga
    54121 tacattacta gaagttcaaa atgacaaatc agtagaagaa atcccgtcac ctgttacagg
    54181 aacggttaaa aatgtgattg ttccagaagg taccgtagca aatgttgggg atgtattagt
    54241 agaaatcgat gcaccaggac acgaagacaa tgaaggggac agtggtgtag ccgcagaatc
    54301 ccaaactcca gctaaaccgg ctgctgaacc cactgttgat acagaatcag ctggatcgtc
    54361 aagcgaagga gtcttccaat ttaagttacc ggatatcggt gaaggaatcg cagaaggtga
    54421 aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat gaagatgata cattactaga
    54481 agttcaaaat gacaaatcag tagaagaaat tccgtcacct gtcacaggga cagttaaaaa
    54541 cgtgattgtt ccagaaggca ctgtagcaaa tgttggggat gtattagtag aaatcgatgc
    54601 tcctggacac aatagtgcac cgtcaactag tgcgccatca gctgaagcac caaaagaaaa
    54661 agtggaaaca tcaggttcag caagtgtcgt tgaagctgcc gatccaaata aacgtgtatt
    54721 ggctatgcca tctgttcgtc aatttgcccg tgaaaaagat gtagatatct cacaagtaac
    54781 tgcaactgga aaaggtggca gagtaacaaa agaggacatc gaaaacttct tggcaggtgg
    54841 tccaagcagt gcaccagcga aatcagaagc gccagaagcg gcagcaccga aagaagcagc
    54901 tcctgcagca gaaagcaaac cagctgcacc agcaaaacca ttcaagtcca acttaggaga
    54961 cttggaagaa cgtgtagcaa tgacgccaac acgtaaagct atcgctaaag caatggtcaa
    55021 cagtaagcat acagctccac acgtgacatt gcatgatgaa gtagaagtgt ctaaactatg
    55081 ggataatcgg aaacgattta aagaagttgc tgctgcaaat ggtacgaaac ttactttctt
    55141 gccatatgtc gtgaaagctt taacagcgac tgttaagaaa tacccagtat tgaatgcatc
    55201 tatcgacgat gcaaaccaag aaatcgttta taaacattac tacaatatcg gtattgcaac
    55261 tgatacggat cacggactat atgtaccaaa tgtgaaagat gcagaccgca aaggaatgtt
    55321 tgctattgca gacgaaatca atgagaaagc aaaattagct catgacggca aactatctgc
    55381 agaagatatg cgcaatggaa caattacgat cagcaatatc ggatctgtag gtggcggttg
    55441 gtttacacca gtcatcaact atccagaagt agctatttta ggtgtaggaa cgattgcaca
    55501 acaaccaatc gtaaatgctg aaggcgaaat cgttgtcggt cgtgtgatga aactgtcatt
    55561 aagtttcgat caccgtatcg tagatggagc aactgcgcaa caagcaatga ataatatcaa
    55621 acgtctttta gctgatccag agctattaat gatggaagga tgataaacaa atggtagttg
    55681 gagattttgc tgtagaatta gatacagttg taattggtgc gggacctggt ggctatgtag
    55741 ctgccatccg cgctgctgaa atgggacaaa aagtagcaat cattgaaaga gagtatattg
    55801 gcggtgtttg tttgaatgtc ggctgtattc cttcaaaagc actgatagct gcaggacacc
    55861 attatcaaga gtcactagat tctgaaatgt ttggagtaac tagtgaaaac gtcaaactag
    55921 attttgcaaa aacacaagaa tggaaagaaa acaaagttgt ccatacgttg acttctggtg
    55981 ttggtttcct attaaaaaaa cataaggttg aaacaatcga aggagaagcg ttcttcgtag
    56041 atgatcacac actacgcgtc attcatccag attcagctca aacttattcg ttcaataacg
    56101 caatcatcgc aacaggttcc cgtccaatcg agattccagg atttaaattc ggaggacgtg
    56161 tgttagattc tactggcgga ttagcattga aagaagtacc taagaaattt gtgatcatcg
    56221 gtggcggtgt gattggtgcc gaattgggcg gagcgtacgc aaacttagga gcagaagtaa
    56281 cgattcttga aggctcacca caaattttac caacttacga aaaagatcta gtaaaattag
    56341 tagaagatga tttcaagaaa aaaggcgtaa cagtcgtaac gaatgcaatg gcgaaagaag
    56401 cagtcgataa cggtgacagc gtgacagtga aatatgcagt ggacgggaaa gaagaatccg
    56461 ttactgctga ttatgtgatg gtcactgtag gtcgtcgtcc gaatacagat gatatgggct
    56521 tagaacaagc gggtgtcgaa gtaggagaac gaggattgat cacggtggat aaacaaggac
    56581 gtacgaacgt acctaacatc tatgccatcg gtgatatcgt tccaggggcg gcattagcac
    56641 acaaagccag ttatgaagca aaaatcgctg ctgaagcaat ctctggcaaa aaagtagctg
    56701 tagattataa agcaatgccg gcagtagcct tcactgatcc tgaattggct tctgtaggta
    56761 tgacgatcaa agaagcaaaa gatgctggta tcgaagcaaa agcatataaa ttcccatttt
    56821 cagggaacgg tcgggcatta tcgcttggta aaacagaagg attcatccgt ctagttacga
    56881 cgatcgaaga taacgtgctt attggcgcgc aaatcggtgg agtcggcgca agtgatatgg
    56941 tctctgaatt agctttagca atcgaatcag gaatgaatgc tgaagatatc gccttgacga
    57001 ttcatcctca tccatctctt ggcgaaatcg tcatggatgc ttcggaatta gcattaggtt
    57061 taccaatcca tatttgattt gaataataca ggcgaaacgc aactgaaaaa tcagttgcgt
    57121 tttgtttttt agaattatca ataatattat tatgtaaact taaaaatatg acaacttctt
    57181 ggtttattac cacttcctat gctacactga agaggaatat gaaataggag agttgaagat
    57241 gcaagaatat cagtatccac ttgatttaga ttggacaaca gacgaaatgg tcattgtaat
    57301 gaagatgtgg gaagatctgg aacgtgcgaa tgaaaaaggg ataaacaatg aagctttttt
    57361 gaagacttat cagcagttta aaactgttgt caaatccatt ggtgaggaaa aacgattggg
    57421 acgggaattt gaaaaagctt ccggttattc tttatatcgt acagtgcagc aagcaaaaaa
    57481 aaatccaaac aaaactttga gaatgaacgg gtgagataaa tggaacagat gattgatcat
    57541 atcaagtcct ggcttgaaga agctggtgag aaaatcaaat cttctcaaca aacattaaca
    57601 atctcgcaaa aatcaaatcg aacggatcta gtaacaaatg tcgatcgaaa catccagaat
    57661 tttctaatcg aaaaaatcca ttcctttgat ccagaggcga aaatattggc agaagaagaa
    57721 gggtgtaatc aattatcgga tctcgatgga agagtcttta tcatcgatcc tattgatggc
    57781 accttgaatt ttgtgattga aggggaaaat ttttgtatta tgctcgctgt ttatgaatcc
    57841 ggcgttggaa agcttggttt catttatgac gtgatgagaa atgaactttt ttggggtgga
    57901 aaaggaatag gcgtgtataa aaatgatgag ccattgccta gaccagcaaa taaatcctta
    57961 tcggaaggct tacttggtgt gaatagttat cttttcggtc ataataaata tggaatccga
    58021 gaaattgcgg aaagcagcat ggggacacgt atgtacggat gcgctggtct tgaactagtg
    58081 gcaatcttga aaggaaacca cattgcttat atttcaaacc ttagcccttg ggattatgca
    58141 gcgggaaatg tcttattgga agagtttggt atggtaacaa gcggtctttt tggtactcct
    58201 ttgacatttg aaaaaaggga atactattta gcagggactt cttgtgcata tgcagaaatc
    58261 gcagaccttt tgaaaaaaga aggctgaaaa aaaggtagaa atcattcaca aaaatgataa
    58321 aaacgagcaa aagattaatt ttctcgaaaa aatgaaaaca ttttactata tttcatgaaa
    58381 aaagtctgct ataattagca gtcgagtaaa atttgccctg tcataaagaa ccaataaaaa
    58441 aggagttagt tacattgaat tatagaaacg atatccgtaa cgtcgcaatt attgcccacg
    58501 ttgaccacgg aaaaacaaca ttagttgatg aactattaaa acaatcagat acattagacg
    58561 cacatacgca attgcaagaa cgtgcgatgg actcaaatgc gctagaaaaa gaacgtggta
    58621 tcacgatttt agccaaaaac acagctgtag actataaagg tattcgcgta aacatcatgg
    58681 atactccagg acacgcggac ttcggtggcg aagtagaacg gatcatgaaa atggttgacg
    58741 gcgttgtgtt ggtcgtagat gcgtatgaag gaacaatgcc tcaaacacgt tttgtattga
    58801 aaaaagcact ggaacaacat atcacaccaa tcgttgtcgt aaacaagatt gacaaacctt
    58861 ctgctcgtcc tgagcatgtc gtagatgaag ttcttgaact attcatcgaa ttaggagcag
    58921 atgatgatca gttagacttt cctgtgattt atgcatctgc actaaatggt acgtcaagct
    58981 tgtcagatga tccagcagat caagaaccaa ccatggcacc tatttttgat acaattattg
    59041 aaaaaatccc tgctcctgtc gataacagcg acgaaccatt acaattccaa gtttcattac
    59101 ttgactacaa cgattatgta ggtcgtatcg gaatcggacg tgtatttaga ggaacaatca
    59161 aagtaggaga ccaagtagca ttaattaaat tagacggaac agttaagaaa ttccgtgtaa
    59221 cgaaattatt tggtttcttt ggactaaaac gtctagagat tcaagaagca aaagctggag
    59281 acttgattgc tgtctcaggt atggaagata tctttgtcgg tgaaacagtt actcctgtgg
    59341 atcatcaaga tgcgttacct atccttcaca ttgacgaacc aacattgcaa atgactttct
    59401 tagttaataa ttcaccattt gccggtcgag aaggtaaatt cgtaaccgct cgtaagattg
    59461 aagaacgttt gatggcagaa cttcaaacag atgtatcctt acgagttgaa ccaacaaact
    59521 ctccagatgc atggacagtt tctggtcgtg gagaattgca tttgtctatc ttgattgaaa
    59581 acatgcgccg tgagggatac gaattgcaag tatctcgtcc agaagttatt gaaaaagaaa
    59641 tcgacggtgt aaaatgcgaa ccgtttgaac gcgtgcaaat cgatacacca gaagaatata
    59701 tgggtagtgt tatcgagtcc ttgagcttac gtaaaggtga aatgcaagat atggtccata
    59761 caggaaacgg acaaatccgt ttgacattct taacacctgc ccgcggtttg attggttatt
    59821 caactgaatt cctttcaatg acacgtggtt acggaatcat gaaccatact tttgaccaat
    59881 atctgccaat gcttcctggt caaattggcg gacgtcacca aggggcgctt gtttcgatcg
    59941 atacaggaaa agcaacgact tattctatca tgagtatcga agaacgcggt acagtcttcg
    60001 tagaaccagg tacagaagta tatgaaggaa tgattatcgg ggaaaacagc cgtgataatg
    60061 atttgactgt caacatcacg aaagcaaaac aaatgacaaa cgtacggtct gctactaaag
    60121 accaaacatc tgtcatcaaa aaaccaaaac aattgacgtt ggaagaatct ttggaattct
    60181 tgaatgatga tgaatattgc gaagtaacac cagaaagcat tcgcttgaga aaacaaattt
    60241 tagagaaaaa tgctcgtgaa aaagcatcta aaaagaaaaa ataatctgta aaaggatgaa
    60301 gagagaaacc agcttgttat aaaaaatagc tggtttcttt ttttgtccaa ctcttttctg
    60361 tggagaagtc atctttgtca atggacataa attcagaaaa tacgccgaaa gacgtttaaa
    60421 agacttttaa aaaacttatt tctagagagt ttttgtttca taataagata atgtttcacg
    60481 aaaggagatc aacttgtcct tcgaagaaaa tcgaagtaaa atggcaagag aaaggagcgg
    60541 gaagatgaac gacttattaa aacaatactt tggttatgat gagtttagac ctggtcaaaa
    60601 agaaattatc cagaaagtca ttgatcagga aaatgtatta ggaatcatgc ctaccggaag
    60661 tgggaaatca atctgttacc agcttccagc tttactatta gatgggctta ctgtggttgt
    60721 gtccccttta atctctttga tgaaggatca ggtggacgcg gcgaatcaat taggtatacc
    60781 cgccactttt atcaatagct cgttagatgg ttacgaaaca gctagacgct tccaagaaat
    60841 cgatagacag cagtaccgtt tactttatat cgctcctgaa cgttttatca tgccggattt
    60901 tatccaagca atgaagcgat ggaacgtccg tatgatcgct attgacgagg cacattgtat
    60961 ctctcagtgg gggcatgatt tccgcccaag ttatttacag atggcgaacc agctagatca
    61021 actgccaaat cgtcctgtaa ttgttgcttt gacagctaca gcgactgtgc aagtagctgc
    61081 tgacataaaa agacttttaa aaattccaga aaacaaccat attcagactg gttttgaacg
    61141 agaaaatcta cgatttcaag taattaaaga tcaaaaaaaa gaacaatatc taatcgaata
    61201 tttaaagatc aacaaaaatc aatctggtat catttatgca gcaacgagaa aagaagtaga
    61261 ccgtctctat catttgctta aaaaattcga tttttccgtg ggtagatatc atggtggtct
    61321 aaatgagaat gagcgaacag aaatgcaaga agccttttta tacgatcgct tgcagctgat
    61381 tgtagcgaca aatgcatttg gtatgggtat aaataaaagc aatgtccgtt ttgttatcca
    61441 ttatcaaatt cctggctctc tagaagcata ctatcaggaa gctggtcggg cgggaagaga
    61501 tggtctttcc agtgaagcca tcctgctttt ctctcctcaa gatatccaag tccaaaagtt
    61561 ttttatccag cagtcccaaa gagaagaagg gcaaaagcaa aaagaatatg aaaaattaaa
    61621 agcgatgaca gaatacgtgt atattgagtc ctgtcttcag caatacatcc taaactattt
    61681 tggcgagaca agcagtccgt gcaaccgttg tggtaactgt ttagatgaca gagaaatagt
    61741 agaagtaacg acagaagcac agatggttct ttcttgctta aaacgtatgg gtgagaacta
    61801 tggtaaacaa atgctgatga aagtcttagc aggatcgaaa gaacaaaagc tgcaagcttt
    61861 aggttttgga catttatcta cctatggact gatgaaaaac caatcgcaaa aagaaacgat
    61921 gcagttaatt gaatatttga tatcgaatgg ctatcttctg accatcaatg gtgagtatcc
    61981 tgttttgaaa gtaacagaaa gagggataca agtattgaaa ggacaagaat ctgtttatcg
    62041 aaaggaaccc aagaaagtcc agcagctatc ggatgaagaa accgatacac tatttgaagt
    62101 gctgcgggaa ctgcgaacgg atctagcatc agaagcaggc gttcccccgt atgtcgtatt
    62161 ttcagattcc acattgaaag aaatgagccg aatacgtcca tcaagcagat tggagatgct
    62221 tcagataaaa ggagttggcc aaagcaagct tgataaatat ggcgaagcct ttttatctcg
    62281 gataaaaaat gcggactcta acgttctgga taagtagaaa gaacgaatac gtaaaaatga
    62341 ataaaaacaa ctggttaaat ctgttttttt tgctccttat gaaaatcaaa tatgttataa
    62401 ttaaggaatt agaatgggag gtaagcacag atggattctt tgtcgaaaaa acttgcgatc
    62461 caagtattaa atgatgaagc agaacggatc aaacgattaa tccgaaatca aaacaacagt
    62521 ctctgtatct cacaatgcaa agccttcgaa gaagtggtag atacacagat gtacggcttt
    62581 tcccggcaag tcggttttgc tgtccgtata ggagtaatcg gaaagaacga gggccagctg
    62641 atgttaagtg agctagaacg agaattgaat cagttatata gtgaggttta ccaagaaatt
    62701 tatgataaaa aagaagttgg caaggaggaa tagacttgcc taagaaagtg aaaaaaaggc
    62761 atctattaga ctatagcatc ttgattccct atttaatttt atgtgtattg ggattgatca
    62821 tggtatatag ttctacgtcg tatctattat tagaaaacgg acaaaatccg tccgcatcag
    62881 tcattaacca gagtattttt tgggtgctta gcttgattgc tatcgcacta ctttataaaa
    62941 tgaaaacaga tgttttgaaa aaccagcgtt tgatcatggc agccatcgca gttttgacga
    63001 ttttgctgct gattgttgtg ttttttggta aggagatcaa tggggcgaaa ggttggctgc
    63061 aaattgcagg attctcgatc caacctgccg aatacctaaa aatcatttct atctggtacc
    63121 tgtctctgac tttatctaaa agacaaaaca gtgtacaaaa agattttctt gggactgtga
    63181 aacgtccact tgcaatggtt atcggactta ctgcattagt cgctattctt cccgattttg
    63241 gtaatgcggc agttatcttt ttgattattt tggttctgct tttagccagc ggagtcaatt
    63301 atgtgtatac tttgattgta ggagtaggag gcttttgtct aagtactttt acgatctggc
    63361 tgatcaatat cacaaacgga aagatcttac ccggacgttt acagtatatc tataatcgat
    63421 ttgctattta ccaaaatcct ttctcagacg aattaaataa agggcatcaa ctagtcaacg
    63481 gctactatgc catgttcaac ggaggactat ttggcagggg attaggtaac agtatccaga
    63541 aaaaaggatt tttacaagaa gcacaaaccg attttattta tgcaatcgta gtggaagaac
    63601 tgggagtgat tatggggatt ttgatattag cgttgctatt ctttatgatt gtgcgcattg
    63661 tccttgtagg tatccgttca aaagatccgt tcaattcgct tttgtgcatt gggatcggcg
    63721 caatgttttt gatccaagta tttgtcaatc ttggaggaat tactggcgtt attcctttga
    63781 cagggatcac gttcccattt ttaagtcaag gaggctccag tttgctgatg ctttcgattt
    63841 gtgtcggctt tgtcctcaac atcagtgcgg atgaaaaacg caagagctta ggtttataat
    63901 aaaaaaagat gtaggagtgg ttcgatgaaa aaagttttag ttgctaacag gggtgaaatc
    63961 gctgtacgtg tatttcgtgc ttgtacagaa ttaggaatta aaactgttgg aatctatgca
    64021 gaagaagatg aatattccgt acaccgtttc aaagcggatg aagcatattt agtaggacaa
    64081 ggaaaaaaac cgattgatgc gtatctagat attgaaggga tcatttctat cgcgaaagaa
    64141 tgcggagctg acgctattca tccgggttac ggtcttttgt cggaaaactt gaattttgcg
    64201 caacggtgcg aagaagaagg catcattttt gttggaccca aactgcatca tttagatatt
    64261 tttggagata aaatcaaagc aaaagctgca gctatcgaag cggggattgc ttcgattcca
    64321 ggcacagatg gaccaatcgc ttctatagac gatgccttgg agtttgcgaa acaatacggt
    64381 tatccgatta tgatcaaggc agctctaggc ggcggcggac gcggcatgcg tgtagctcac
    64441 gatgaaaaga gtgcaagaga gggctacgaa cgtgcgaaaa gcgaagcaaa agcagccttt
    64501 ggtagtgatg aagtgtatgt tgaaaaatac atcgctaatc ctaagcatat cgaagtacag
    64561 attctcgggg atacgcatgg aaacgtcatc catctttttg aaagagattg ttctgttcag
    64621 cgccgtcacc aaaaagtagt agaagtagcg ccttgtgtct ctatgaatga acagcaacgt
    64681 caaaaaatct gccaggcagc tgtccaattg atgaaatatg tcgggtatgt caacgcaggt
    64741 acagtagaat ttcttgttga aggagatgat ttttacttta tcgaagtcaa ccctcgcgtc
    64801 caagtggaac atacgatcac agaaatgatt acagatattg atatcgtaac aacgcaactg
    64861 ttgattgctc aaggtttgga tctgcataaa gaaatcggat tgcctcagca agaaggaatt
    64921 aaattaaatg gttcagccat tcaatgccga atcactacag aagacccgtt gaataacttt
    64981 ttaccagaca cagggaaaat cgatacgtat cgttcacctg gtggttttgg cgtacgtctg
    65041 gatgtcggaa atgcttacgc gggctatgta gtgacaccgt attttgattc attgctagta
    65101 aaagtatgta cgcatggtgc tacctttgaa acagccattc agaaaatgga acgttgcttg
    65161 aaagaattta gaatccgtgg agtgaaaaca aacattccat ttatgctcaa tgtcatcaca
    65221 catcctgaat tccaatcagg aaatgcaaaa acaaccttca ttgatagtac agcaacatta
    65281 tttgaatttc ctcgactacg agaccgcgga aacaaaacga tgaaatatat cggtgaaatt
    65341 actgtgaatg gttttccagg tatcgaaagt ggcgaaaaac cattttatga agaaccacgt
    65401 atgcctaaag atctaatcac aagaactgat tacgtaacgg ctaagaatgt attagatgcg
    65461 aagggtgccg atgctttggt cgaatggatc aaaggacagg aaaatttgtt gttgacggat
    65521 acgacattta gagacgccca tcaaagtctg ctagcaactc gtgttcggac aaaggatttc
    65581 aagcagattg cacgtttgac tggagaaggt cttcctgaac tcttttcaag tgagatgtgg
    65641 gggggagcaa catttgatgt ggcttaccgc tttttgaatg aagatccttg gcaacggttg
    65701 agaaaaattc gttctttaat gcctaatacg ttgctgcaaa tgcttttcag aggttcaaat
    65761 gccgttgggt attctaatta tcctgacaat gtattggtgg aatttgtcaa agaagcagca
    65821 gcgcaaggaa tcgatgtatt tcgaatcttt gatagtttga actggactcc acaaatggaa
    65881 aaaagtatcc aagctgtacg ggatactggt aaaatcgcag aggcagctat ttgctatact
    65941 ggcgatatca acgatccaag tcgggcaaaa tacaatgttc agtattataa agacatggca
    66001 aaggaactag aacaactggg tgcgcatatc attgcaatca aagatatggc tgggctgctt
    66061 aaaccgcagg cagcttatcg tttgattagt gaattgaaag aaacgacaga tctgccaatc
    66121 catctgcata ctcatgatac cagcgggaat ggaatcatca cttattctgc tgcttcaaaa
    66181 gcaggagtag acatcgttga cgtggcaatg agtgcaatga gtgggaatac tagtcagcct
    66241 agcatgagca gcttgtatta tgcactggtc aatggtccac ggctgccaga aatcacgatt
    66301 gaaaatgcac agaaattaaa ccattattgg gaagatgtgc gcatgtatta caaaccgttt
    66361 gaaaacggct tgaatgctcc tgaaacagaa gtatatatgc atgaaatgcc aggaggacag
    66421 tattcgaatc tacagcaaca ggcaaaagct gtcggattag gtcatcgctg ggatgaaatc
    66481 aaacaaatgt atcatactgt caacttgatg tttggcgata tcgttaaagt aactccatca
    66541 tctaaagtag ttggagatat ggcactattt atggtccaaa atgatcttac agaagaagat
    66601 atttatgaaa aaggcgaaac actaagtttc cctgaatctg ttgtgacttt cttccaagga
    66661 gaactagggc aacccgttgg cggatttcct aaaaaattgc aaaagattat tcttaaaggc
    66721 agacctgcat tgaatgaacg tccaggtctt ttggctgaat cagtcgactt caacgaagta
    66781 aaaaaagaat tggcagaaaa aattggctat gaaccaaaac aagacgaagt attaagctat
    66841 ttgatgtatc cgcaagtctt tttggactat caaaaagcat ataatcaatt tggcgatgtt
    66901 acattactgg acacaccaac atttttccaa ggtatacgtt tgggtgaaac aatcaatgta
    66961 caaatcgaac gcggaaaaac gcttatcatt cgtttagatg aaatcggaga acccgatatc
    67021 gaagggaatc gcgtactgtt cttcaatttg aatggacaaa gacgtgaaat cactatcaaa
    67081 gataattcga tcatcagtac ggtccaaacc cgattgaaag ctgaaccgac aaaccgagaa
    67141 caaattggag caacgatgtc tggttctgtt ttggatgtat tagtgaaaaa aggcgataac
    67201 gttaaaaaag gtgatacgtt gatgattact gaagcgatga aaatggaaac ggccatcgaa
    67261 gcacgttttg acggagaagt cgcacatgta tatgtgtctt caggagacac tatttcgtct
    67321 ggtgaccttt tgattgaagt gactgagaaa taataagcga aaggtgggga aacatgaaaa
    67381 gactgggttt gttttggctg attctgctat tagttattgt ggcattctac atggaaccag
    67441 tcttgtttcc gccaaaactt gaagtgaagg aaaactctga tcaacaaata tcaaaccaaa
    67501 tgacaacgtg gaaataccag gagcttaaag caactggttt ttctacttat atcggaaaac
    67561 cgatcgatca gctggaaaag gattttggcc aagcctatga tcgattaact agcggatttg
    67621 gttttgaaac aagatattat gaaaataaag ctagtcatct cacatttgaa gcgaacatcc
    67681 aagacagcag agtcacaact gttaaagtac tgcaaacagg gcctgctgat atcgcgccgt
    67741 tttatttagg gatgacgatg caagatctta cgaaaatcac tactatctat actaatttca
    67801 cttttgagta ccatgataca gaagttggaa tcgaactaat ggaagaggat atgaattatc
    67861 gtccgctaat tgcttttgat aacgacactt ttgctatttt attttttgat caaacaacaa
    67921 acggcctatt ttcagttgtg tatttagata aagatagtct tttgaaacta ttgccatatc
    67981 aagtgtttgg tgaaggcctt ccacattatc aagcagataa atcggctgat tgggaatcaa
    68041 tcaatcgcgt taaagaacga aaaagtacga ctttattaaa tatgcttaga agagaagacg
    68101 aacttcccat atacaatcaa acacttgcct ttcaaaacca cagtcagctg ctactacatg
    68161 attttttgac aaggcctgag gaaatattgt cagaggaacg tatggctgat tggcaaaaat
    68221 cgctaggcag tgctcaaacg acagtgaaat tctcactgac gaatgatgaa ttggagaagt
    68281 taatcagtaa acaaaaatta gaaaatacta gtgggctatt tattcatccg gcgattgacg
    68341 ctactttttc gtttttatat tggtacagcg atccttacaa ccatgaccgt ttcatggacc
    68401 cgggaacgga ttctgtaggc attgcttttt ctaaagaaaa tatgttagtc ttattgcaag
    68461 aagaaaacgg agaaagtagt gatagcagtg atcgttaacg aagagctgtt tcaactggag
    68521 gatcaatgcc tccaagtcgc tgaaatgata aaaaagagta aaacgatggc ttcttatctt
    68581 gaacataaac agtcaatgga agaatctgca aaagtttgcc agttgaaaga agcgttcttg
    68641 tctaagaaag aagcatttga aaatgtagcg gcatatggtg agtatgcacc tgattatcgt
    68701 gaaaaaaaac gagctgttcg aaaagcaaaa agacagcttg atatggatga gcatgttgca
    68761 gctttccgat atagtgaaac acagctgcaa agtctgttgg atcaaattgt ccaagtactt
    68821 gccgaagaag tttctaatga aatcaaagta gatgcaggta gcccgttttt tgaaacgaaa
    68881 aaacactttg gatgcggagg taattgtcat gcaattagac aatagtgaat ttgaaataac
    68941 aaaacgacgc gggctgatcg tatgggtcta cagcttaaaa caattaaaga atctgaaaaa
    69001 gttcggcttg ttgcattatg tctcaagaag aatgaaatat gtcgtgcttt acttaaatga
    69061 agagacattg gaagaaacag aagaacgaat ccgtaaatta cattttgttc gaaacgtaga
    69121 acgttcttac cgaccggata tcgaaatgaa ttttgccgaa aaaataggaa acaaaacagt
    69181 attaaaagac gaaggtttcg aaattgaaga gctaaataca gaaatccgat tagctgaaca
    69241 tctttaaaaa tctcttatga gcagttttta aataaaaaat aacttggata acgatcgatt
    69301 tttgatcatt gtccaagtat ttttttggta tactagtacc agtaccattt ctaaggagtg
    69361 agtagatgcg gataatatca ggaaattatg gcgggcgccg tttaaaaagt ttagcagggg
    69421 cgaacaccag accaactaca gataaagtaa aagaatccat attcaatatg attggaccat
    69481 attttgatgg agagacagta ttggatctgt ttgcaggaag cggtggttta gctatcgaag
    69541 cagtatctag aggctgcagt catgcggtat gcgtagacaa aaattaccaa gcgctaaaaa
    69601 tcatcaaaga aaacatcgaa attacgaaag aaccagagaa atttaccaca ataaaacttg
    69661 atgcggacaa agcaatcgaa cggctagcag cggagaaaca acaattcgac tacttgtttc
    69721 ttgatccgcc atatgcaaaa caaaaaataa tcgatcaaat ccataaaatg gaagaactgg
    69781 gacttttttc taaaaccgca gttatcgtat gcgaaacgga taaaacagtc gaattgccag
    69841 aaactgtggc tggattccat caaataagaa aacagattta tggcataacg gccgtaacta
    69901 tctatagaaa agagggaaat caatgaaacg tgcattattc ccaggaagtt ttgatccttt
    69961 tacaaaaggt cacttagata cggtggaacg tgcggctaaa ttattcgatg aggtagtaat
    70021 tggtgttttt atcaatactt caaagaaaag tttgtttccc ccagaagaaa gaatgacatt
    70081 gatcacaaaa gctgtttctc atcttcctaa tgtaaaagtc atgcatcaag aaaatcaatt
    70141 gacggtagaa acagcaaaag agattggtgc agatgcgttg atcagaggca tacgaagtat
    70201 caaagacttt gaatatgaac gagagattgc tcagatgaat catcatctat atgatgaatt
    70261 ggaaactgtc ttcttattag caaaaccaga atatagtcac gtgagttcca gtattttaaa
    70321 agaagtactg cactttggcg gagatgtgag cagctactta ccgccagtga tcaacgaagc
    70381 tttagctgga aagcgtgaat caaatgaaag ctaaacaatg gttgaaaaca gtactgatta
    70441 tcatatttgg aatgttgatc attggatttg tagtagttcc cttgccgtat tatgtagaat
    70501 caccgggagc cacgatcgat ctgaaagaac taattacggt caatgataag gaagataaag
    70561 gttctgggtc tttctctctt acctctgttg gtatcaggca agctacaggt ttcttagctt
    70621 taaaagctaa aatgacccct tttgaagaat tgatcagtga agaagaattg actggtgggg
    70681 cgacaaatga agaatataat caaatgcaac agtattatat ggattcttcc caaaatgcag
    70741 caatcgaaca agcgttaaaa cttgcagaca agccttataa aatggaattc aaaggcgtat
    70801 atgtcatgag tgtagagaaa acatcgaatt tctttggcaa gctttcagtt ggagatacag
    70861 taacaaaagt agacgggaaa agctttcaaa gtacagaagc atttatggat tatgtaaaaa
    70921 gccaaaaagt tggacaaaca atcgaaatca gttatttgca taatggtgaa gaaaaaacag
    70981 cttctggtga tctgatcgag ttaccaacag ataaaaaggc aggaataggt atcacgctta
    71041 ctgaccatac cgagatcgaa tcagatacag atgttcgtat taattctggc tctatcggtg
    71101 ggccatcagc gggattgatg ttcactttag aaatctatga acaagtcact ggtaaaaact
    71161 tgcgacatgg taagcaaatt gcaggaactg gaacgatcaa cagtgaagga gaagttggcc
    71221 gaatcggagg aatcgataaa aaagtggcaa gtgcggataa agctggcgta gagattttct
    71281 ttgcaccaga tgatacgatt tcagacgatg ttaagaaaga atatcctgaa ataaaaacca
    71341 actacgaaga agcaaaagct gccgctaaaa aattgaattc ctcaatgaaa atcgtaccag
    71401 taaaaacagt acaagaagcg cttgactatc tagagaaaat gaaatgaaac taaataatta
    71461 aagaggggtt gtgacacgag ttctgtcaca acccctcttt ttcgtcaaaa atcttcacga
    71521 taaaaataaa aacaaaagaa aaaattgacg taattagatg ttgaagggac gattttgcca
    71581 gagctcactg tttcttcaat tttaaatgaa aggagtaatg aatgattttt actggtcatt
    71641 ctttgaaaaa agaacaatga tagaaaaaat cagcaagaaa atactagctt cacccaaatt
    71701 gatcatttgg atgtctatga tagttcttat cttttgtatc gcaagtggtt ttttattatc
    71761 aaatttgcta ttttatagag aaacagaaga gactgctgca aaagaaatac tttcttcgtt
    71821 tagttccagt ggagaagatc catcagctga aggaaacgat gtagaagagg atgctcgtcc
    71881 aaaggtcatg tacaccgata tcaaaggaag tgtgaaggag cctggtatct attccttttc
    71941 ttcagaagaa cgggtctatg atgtactaaa gcgtgcaggt ggattattag aagaagcgga
    72001 tagcgatcga atcaactttt ctgcgaagat agaagatcag caagtcctat atatccctgc
    72061 tgtaggtgaa gagctgccag aacatcttga tcagttggca tcctcggaag gaaaacagcc
    72121 tacagcagat tccgagcctc ccaaaataaa tatcaataca gccactccat cagaacttca
    72181 gcagattcct gggatcggat cagtcaaagc gcaagaaatc attcgattta gagaagaaaa
    72241 cggatccttc cagaaagtgg aagacctgca ggagatttct ggtatcggtg aaaagacagt
    72301 tgaaaaacta aaaaacttcg ttacaattaa atagaatgat tcaaaatcga ctggcacgag
    72361 caataaatgg acgctaatgg atactaagaa taatccaatt ctaagagctt ggcaggacga
    72421 ggaggtagta tttttggata ggaaatagct tgattgttcg caaagaaaca ttaaaaaaac
    72481 caatgctgaa tgtctatctc caaaataaaa tatctgggat tcatattatg aatacggctg
    72541 tcagcggcaa taacagtcaa gctctacgag aacgctttgc caaagatgta cttagctata
    72601 cagcagataa agtatttatt ctcattggga cgaatgatct agcagagcat aaacaacttt
    72661 ctaaagaaac gtaccaaaaa atctgcagtg gatgatagtt cagctgaaaa aagactatag
    72721 ttctgatcat gctttcttta tcacgccacc agcagtcgat gagacaaaac aaaaatatcg
    72781 gaataacaag ttgatcaaag aatatatctc tgttctcaca gaagtagcaa aacgccatga
    72841 ttgtgaagta tcgattttta tacggaattt cttgaacaaa tggattcgaa gaatttttat
    72901 aatgggctaa tggatgacgg acttcatttc ggcagtgaag ggtaggatct tttgtcagac
    72961 aggatgattc gttatattta aagaatattt tgtttaagca atgagtactt tttaatcaac
    73021 ttgaaaaaac ggaggaatat aaaacatgtt agaaaaatct tcaaaagcat gcgatacgaa
    73081 taaagaaaaa gttactgata agtcacataa tgaacgcatt ccttgggacc agtactttat
    73141 ggctcaggca gtactgttat ctttacgtag tacgtgtaca cgtttagaag taggagcgac
    73201 actcgttcga gaaaaaagga tcattgctgg cggctataat ggagcagtca gtggagatgt
    73261 ccactgcata gatgaagggt gttacgtcgt ggatggacac tgcttgcgaa cgatccatgc
    73321 agagatgaat gcactcctac agtgtgcaaa acttggtatc ccgacggaag gttcagaaat
    73381 ctatgtcaca cattttccat gtttggcctg tacaaaggct ttgttgcaag ctggtgtgaa
    73441 agggatcaat tatctccatg actatcgaaa tgatccttat gcccaagcgt tgatcgaaca
    73501 attaggtgta tctgtacacc aagttgcttt agactcacag catttttcaa aacttcaaaa
    73561 tgaattaaca ggaaaacatg tctagcgttt tagaaaggca tgttcgaaat cggttgattt
    73621 ttccggcaat tacgtcgatg attatctgtt tgttgtatta cactgacaat tggggttatc
    73681 gtttgcttct tgccggaatt tttttatggt tttcgctaaa agaaggacgt tttatcgcgt
    73741 acttgtgtat tttgtgtagc ataacggtat ccatttctta taggctacaa gagtattcat
    73801 ttttagagga aaaaacaaca tctgcgcaaa ttactgtatt tacagatacg attcgtgcaa
    73861 atggtaattt tttgacaatg gaaggaaggt tatcagagaa taaacaaaag ctccagcttt
    73921 cttatcaagt aaacacagaa gcagaattga aaacatggct taacttccga ggaaagggaa
    73981 tagaattgac tgctgaaggc gagttcatct ctccagcaaa aaagcggaat ctttataatt
    74041 ttgaccaaga agcttacttc aggaaccata gaatttctgg acggttccaa attcaaaaga
    74101 ttgaacagat caccatgcaa aatcactggc gaaactggct tcagagaaaa agaggatctt
    74161 ttatctcctt tgtccaatct gaatttccgc aaaaaacaag tgtatatatt aattctttat
    74221 tatggggcta taaagatgca ggattcagag aaccagaaga aatttttagg gagagtggtt
    74281 tgctgcatct gttcagtcta tcgggtctac acatccaatt ttatttaggg tggctgtatt
    74341 atttatttcg acgtatcgga tggccactac acgtcagtat tattccgttg agtctactaa
    74401 ctatctgtta tgtttctatt gcaggaagtt ctatttctgt tttgcgtgct agtttgctgt
    74461 ttttattgcg attgctagtc aaattgctga ataagcatta ttccaccatg gacttatttg
    74521 cagttgtctt ttggcttctt ttactatttg atcctcgatt tctttttcat gcgggtggac
    74581 agcttagttt tttcatgagt tttttgttta tcctgatttc ttttgatgga gattggttga
    74641 aaaaaacggc ctctcacgtc ctgtttacta tgatcacgat gccgttgatt gtatggcatt
    74701 tttatgagtg gcctatttta ggtagtcttt ttacattact gattgcaccg gtattcaagt
    74761 ttctttttct tcctggtgtg ttatttctca cactcttcaa tcgctggctg ccggaaactt
    74821 tacttttgct gatagaagag ggattaatct tatttgagcg aataatcgat ttttcttcag
    74881 ggtcgtctct cattatagga cgtttgcccc ttgtgctatc tgggatgtat attttcggat
    74941 tactgatttg tatggatagg ttgaaagaac aacctgtgaa acacttgttt tatgttggtc
    75001 ttttttcttt tatttttttg actttgccat ttttacgctt ttcttcaacg atcgctttta
    75061 tcgatgtcgg tcaaggagac agtatctttt tgcaaagtcc ttttcgaaaa gaaacgattt
    75121 tgatcgatac tggaggtcag ttacactttg aaagagaaaa gtgggcgcaa ggtatgatac
    75181 gtcctcctgc tgaaagaaat atcgttccat atctaaaagg tcaggggata agtgtaatcg
    75241 ataaactcat cttgacacac gacgatacgg atcatgtggg agaattaccc acgttatctc
    75301 agcattttac tatcaaaaaa atttatatcg gttggggcgc tggacgaaat gagccattac
    75361 aaaggcattt atctgaagct caaaaaaatg gtactgagat cattgaaatc aaacatgggg
    75421 acaggatcag cggttattat gatctttatg tattgactcc ttttgaaaag ggagagggaa
    75481 gaaatgaaga ttcgattggt ctatggatgg aatataacaa taggcgcttt ttatttttag
    75541 gagatttgaa tcaagcaatg gaaaaacaat tgcttttgat ttatcctaat ctgaaagccg
    75601 atgtagtcaa attaggtcat cacggtagcc gtacgtcaag taacacagat tttctttccc
    75661 atattgaagc aaaacatggg attatatcct gcggcgtaaa taatcgttat ggtcacccgc
    75721 acagcgaagt attggagaca ctagaagaaa atcagataaa aacatggcga acggatcaac
    75781 aaggaatgat cacctatcgc tggcatccag tttttcatcc tcatgggcta gtggaaacaa
    75841 tgatagacta gtaattctca tctgctcatg gtaggataat ttttgaaagt ggtgagtaat
    75901 ttgagtatac aagcagagtt acaaaaaata cgtaaagaaa aacttgcatc ttgttacttg
    75961 attttaggaa cagaaaagtt tctccaagat caagttcgga cagaaattgt caaaaaaatc
    76021 caattagagg gcgaagatga cttaaatttt ttaagttttg acatggaaaa tgcaagttta
    76081 gatgaggtag tagctgaagc agagacgttg ccgtttttcg gagatcaacg gttagtattc
    76141 gtagagaatc cttatttttt aacaggtgaa aaagtaaaca atggaatcga acaaaatact
    76201 gatttattga cggcttattt gaaagaacca ttagaatcga ctgtattagt attctttgcg
    76261 ccatatgaaa agttggatga acggaaaaaa gtaacgaaac aattgaaaaa aacagcaatt
    76321 atcatcaatg tccaacagtt gaatgaaaaa gaagtacgtc aatatctgat gaatacctta
    76381 gagaattcct caatcaaaat ggaacgaagt gcaattgatt tattcttgag gttgaccgac
    76441 ttagatttat ccaaaatgat gagagagtta cagaaattgg tcctttatgg acaaaatcaa
    76501 cagcaaatca cggtaagaga agtagaagaa ttagttccta aaacgttaga acataatatt
    76561 tttgacatga cccaatatat tttaaaaggg aagacggaac aagctttgcg tttatacgaa
    76621 gatttagtta tgcaagggga agaaacaatc aaaattaatg cgattttgct ttctcaatta
    76681 cgtttatttt tacagacaaa atttcttgta aagattggtt atcagcaggc aaatattgct
    76741 gaaacattga aaatccatcc atatcgtgta aaacttgcca tgcaggaagt aaaaaaattc
    76801 gatgaacatt tgctggtaca gttgtttgat caattagttg agatggatta tcaaatcaaa
    76861 acgggacaaa tcgaaaaaga attaagtttt caactattcg ttttgcgcac agggcagttg
    76921 ttaagaaatc gataagaaga aagacgaggg caaagcatgc gggaaggtcg tttgtttttg
    76981 tccttttttt gacaaataaa aaaatcggcc gaagccgatt cttttattta gcgatttttt
    77041 tgcttaaacg agatttgtcg cggttagctt tgtttttgtg gattagacct tttgattcag
    77101 ccatatcaat agctttaaca gcttctttat ataacgcatc tacgttgtca gcacctgaag
    77161 ctacagcatc ttcaaatttt ttgattgcag tacgcatagc gcttgtttga gatgaatttt
    77221 tcacattagc gttttcgctt gtacgaacac gtttgattgc agattcaatg tttggcatta
    77281 gtttcacctc cgataactta agttcttacg cgtgttaccg cataattcaa cattcattat
    77341 tatacctaaa ggcagcgtgc tttgcaatat gaacatgaca agtttttttc cttaacagtc
    77401 ttttattagg attttttttc agtaaaaacg gatagtacaa gctgaaaaaa atcaaaaaga
    77461 cgctattgtg ggaggcaata gcgtcaaacc aaactagatt tttatttatc tatttaaaca
    77521 aagtttgaat gggaatgttt ggtactaagt tattatataa caaagagaac cgtttgcaaa
    77581 gttttttttg aaaagggata cgattccctg ttgttctttg tttttctatt aagaaactgc
    77641 agttatttta taaccagtat cctttgatca gtaaaggcta ttctggtgta ccgattattt
    77701 taatgtattc atcgatattt tttatcaaca ttttttttat gaccaactgg ttccgacggt
    77761 tccatttgcc acttacttcc agcacatatt tatttagggg caaacgtaag atatattttg
    77821 ccaaatagcg atctttaagc aggccattga tcacacggtc ctcaagctgc aagttgatcc
    77881 cagtcagcat cattggctgt tcttgtaaaa cgtacgtatc aattaaaaga ccttcaaaag
    77941 tttccacaag cgtcttcctt tcttttcaaa gtaacctgaa aacaggcact aattcttcca
    78001 ttttgatatt cgttgaagtg gatgcgatca ttctttcttt tgtccactgt aatctagtat
    78061 aacaaaaaag agcagaagag ccaaaaagaa aaaagagcat cgaattctat aggaagtctc
    78121 attggcacgg cctaaattat tacaaaacat tgattaaaaa aagcccctgc atcgcaggag
    78181 catcccctat atcgttttaa taggtcaatc aaaaagcagt atcaaacaca ttttgtatca
    78241 aatcaggaaa taatagataa gctaagccca gaatcaaaag taccgctaga agaaaccagc
    78301 cgattgttcc tatgaaatac tttaattttt cgaacattgt atcacctcgt tgtcagtata
    78361 tcacggatca gtaaattggg ggtgttaaga atctcctaaa atagacaaaa tttgttctat
    78421 tttcctaaag caaatacttg taagatacag taagtgatac cagaagcaat gaccggtccg
    78481 gcggcgattc ctcttaagaa gacaacacct aaaatcgtgc cgaaaacaag tgcaacagtg
    78541 atttgtggat cagaagcaat caatcctaca cccttggaag ataagacagc cactagccca
    78601 ccgcaaaaaa cagctaccca gccaataggg gatttaaagg cttctaatag atcacgtaga
    78661 ccgatttctc ctgttgcgat agggattaga atagccgctg tgatgatcgt caccccaata
    78721 ttgattcctt tgccttgaag ccaagggaaa aaggaacggg aggctggtat gagcttcata
    78781 atcaaaacaa aagctgccgc tatcatcaga ctttgatttt tagctaaaat tgctacaagt
    78841 aaaataattc ctaaaaaaag ccaactttcc atgaaatata ttcctcactt tcttcgaaac
    78901 aatcataaca tgtttttaga aagttacaaa agtaggaatt catccttcaa gacctattca
    78961 taagctgttt ttttcataaa ataaacaaaa tcataaaaaa acactttgct tatttgacag
    79021 aagacagccg cgctttgtat agtggacaag acttacattt ttttgaagaa tcagaaagat
    79081 caggtaaagg tcttagtaaa cgcaagccat tcagaataac caatattgtg cttccttcgt
    79141 gtcctacaac tccaagaggt agatttaaaa attgcaggaa gttagaagca atcaacaaga
    79201 gaataacaag aatcgaaaaa ataatatttt gtttgatgat ccgccgcatt tttaaagata
    79261 gccgatgact gtagacaaat ttatttaagt cgcttttcat taagacgatg tctgcgatat
    79321 ccatagcggc atctgtccct tgccccatag cgacacctaa agaagcggct gctaaagcag
    79381 gcgcatcatt gactccatca ccaaccatcg catttacttg gtaaagttca ttctcttttt
    79441 tgactagttg tgttttttct tccggagtac agcttgcata gtaatgttca atccctactt
    79501 gttcagcaat ggcctttgct gtgccagaat ggtcgccggt tatcatactt gtatggatgt
    79561 tttgggattt aaagtaggaa attacttgtt gggtatttgc ttttggaata tctagcagac
    79621 ctaaaacagc aattaactgc tgatctttag aaagataaat cacggttttg ccttgatttt
    79681 ccaaacgttc gatcatttcc gcaatctctt tgctgattat catcgtctca ggatcatatg
    79741 cgtgcttgcc aactttccat tgcgcaccta aataggtagt ttgtaatcca aaaccagtaa
    79801 tttcttctat tggtaagtat tggatttctt caggtattgt gcaatcgaaa tattgcaaaa
    79861 tcgcatgagc aagtggatgc gtcgtttttt gttccatagc aacaagaagt tgttgtgcca
    79921 aatccttttc atcaaagaaa aagctgtcag ttacaactgg ttttccttta gtgagcgttc
    79981 ctgttttatc aaaggcaatc gcttttaatt cagaaagctt ttctaaatgg ataccgcctt
    80041 tgattaaaat cccatttttt gcagaatttg aaagagcagc tagagaagct ggtgtagcag
    80101 atgcgacaag cgcacaagga gaagccacaa ctaataatac cattcctcga taaaagcttt
    80161 catcccaact ccaacctagg aaaaagtggg ggagaaggat cattagagga acagcaagca
    80221 aaatcaattt tacataatta ttttcgattc gttcgataaa tgaagctgtt tgagttggcg
    80281 tattctgcgc ttcttctact aattggataa tcttagcaaa gacagtttcg gaactcgttt
    80341 ttgttactcg aatcgtgatg ggatttcctt gattcagcgt tcctgcatac atctcatcgc
    80401 ctaaatattt ttctacaggc acagattcac cgcttaatgc agactcatcg atcgttgcct
    80461 gtgtgctttc taaaaagcca tctatcggaa cagctgctcc tttagcgaca aataccaagt
    80521 tatccacctt tagttgagaa acatctactt cttgcatttg tccattttct tttaataatt
    80581 gggctttttg tggttgaaga ttcatcaaag cagtaatttc tttttgactt ttattagttg
    80641 tgtattcctc taatgcaccg cttaaacaaa agatgaatgt caacattgct ccttcaaagt
    80701 aattaccgat caaacaggca ccaatagcag ctaaagccat caataaatcc acattcagtg
    80761 tccgatcatt ccacaattct ttcaagcctt ccgatgtttg tttatatcca ccgatgataa
    80821 tggcactgat ataaaaaagt ggggtgatgg taagagagaa tcggtcgaag ataaaaccga
    80881 agatcataaa taaaaggcag ccgattgttg ctgcaaaagc ttttttctga ttagtcatcg
    80941 ttattagacc tccttgtcaa aacaaggata gcataataaa aatgtaattg ataataataa
    81001 cagctaaatg agaacgataa tggttatcat ttggtgatta agcttgatgt tgtttttatt
    81061 atttataatg attattatta aagaaaataa aaaaccagaa catttctcaa ataaga
//
LOCUS       DACDRK010000005        78972 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDRK010000005 DACDRK010000000
VERSION     DACDRK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.005"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..479)
                     /gene="dfrG"
                     /locus_tag="IWJ77_000397"
     CDS             complement(<1..479)
                     /gene="dfrG"
                     /locus_tag="IWJ77_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9452047.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYE"
     gene            841..1881
                     /locus_tag="IWJ77_000398"
     CDS             841..1881
                     /locus_tag="IWJ77_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9452048.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1979..2167
                     /locus_tag="IWJ77_000399"
     CDS             1979..2167
                     /locus_tag="IWJ77_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9452049.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2225..2578)
                     /locus_tag="IWJ77_000400"
     CDS             complement(2225..2578)
                     /locus_tag="IWJ77_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9452050.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3109..3531
                     /locus_tag="IWJ77_000401"
     CDS             3109..3531
                     /locus_tag="IWJ77_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9452051.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3528..3758
                     /locus_tag="IWJ77_000402"
     CDS             3528..3758
                     /locus_tag="IWJ77_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9452052.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4256..4456
                     /locus_tag="IWJ77_000403"
     CDS             4256..4456
                     /locus_tag="IWJ77_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9452053.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4483..5676
                     /locus_tag="IWJ77_000404"
     CDS             4483..5676
                     /locus_tag="IWJ77_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9452054.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5744..7309)
                     /gene="guaA"
                     /locus_tag="IWJ77_000405"
     CDS             complement(5744..7309)
                     /gene="guaA"
                     /locus_tag="IWJ77_000405"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9452055.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(7368..8291)
                     /locus_tag="IWJ77_000406"
     CDS             complement(7368..8291)
                     /locus_tag="IWJ77_000406"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9452056.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            8487..9092
                     /locus_tag="IWJ77_000407"
     CDS             8487..9092
                     /locus_tag="IWJ77_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9452057.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            9400..10416
                     /locus_tag="IWJ77_000408"
     CDS             9400..10416
                     /locus_tag="IWJ77_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9452058.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            10557..11858
                     /locus_tag="IWJ77_000409"
     CDS             10557..11858
                     /locus_tag="IWJ77_000409"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9452059.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11904..12566
                     /gene="deoC"
                     /locus_tag="IWJ77_000410"
     CDS             11904..12566
                     /gene="deoC"
                     /locus_tag="IWJ77_000410"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9452060.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            12587..12985
                     /locus_tag="IWJ77_000411"
     CDS             12587..12985
                     /locus_tag="IWJ77_000411"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9452061.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            13051..14133
                     /locus_tag="IWJ77_000412"
     CDS             13051..14133
                     /locus_tag="IWJ77_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9452062.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            14345..15907
                     /locus_tag="IWJ77_000413"
     CDS             14345..15907
                     /locus_tag="IWJ77_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452063.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15900..17033
                     /locus_tag="IWJ77_000414"
     CDS             15900..17033
                     /locus_tag="IWJ77_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452064.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            17033..17989
                     /locus_tag="IWJ77_000415"
     CDS             17033..17989
                     /locus_tag="IWJ77_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452065.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            18088..19254
                     /gene="deoB"
                     /locus_tag="IWJ77_000416"
     CDS             18088..19254
                     /gene="deoB"
                     /locus_tag="IWJ77_000416"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9452066.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            19278..20096
                     /locus_tag="IWJ77_000417"
     CDS             19278..20096
                     /locus_tag="IWJ77_000417"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9452067.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            20113..20820
                     /gene="deoD"
                     /locus_tag="IWJ77_000418"
     CDS             20113..20820
                     /gene="deoD"
                     /locus_tag="IWJ77_000418"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9452068.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(21118..21804)
                     /gene="gpmA"
                     /locus_tag="IWJ77_000419"
     CDS             complement(21118..21804)
                     /gene="gpmA"
                     /locus_tag="IWJ77_000419"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9452069.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(21976..22557)
                     /locus_tag="IWJ77_000420"
     CDS             complement(21976..22557)
                     /locus_tag="IWJ77_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9452070.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(22690..23373)
                     /gene="rpiA"
                     /locus_tag="IWJ77_000421"
     CDS             complement(22690..23373)
                     /gene="rpiA"
                     /locus_tag="IWJ77_000421"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9452071.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            23692..24105
                     /gene="rpsL"
                     /locus_tag="IWJ77_000422"
     CDS             23692..24105
                     /gene="rpsL"
                     /locus_tag="IWJ77_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9452072.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            24189..24659
                     /gene="rpsG"
                     /locus_tag="IWJ77_000423"
     CDS             24189..24659
                     /gene="rpsG"
                     /locus_tag="IWJ77_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9452073.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            24753..26840
                     /gene="fusA"
                     /locus_tag="IWJ77_000424"
     CDS             24753..26840
                     /gene="fusA"
                     /locus_tag="IWJ77_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9452074.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            27012..28199
                     /gene="tuf"
                     /locus_tag="IWJ77_000425"
     CDS             27012..28199
                     /gene="tuf"
                     /locus_tag="IWJ77_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9452075.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            28606..28914
                     /gene="rpsJ"
                     /locus_tag="IWJ77_000426"
     CDS             28606..28914
                     /gene="rpsJ"
                     /locus_tag="IWJ77_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9452076.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            28945..29574
                     /gene="rplC"
                     /locus_tag="IWJ77_000427"
     CDS             28945..29574
                     /gene="rplC"
                     /locus_tag="IWJ77_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9452077.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            29602..30225
                     /gene="rplD"
                     /locus_tag="IWJ77_000428"
     CDS             29602..30225
                     /gene="rplD"
                     /locus_tag="IWJ77_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9452078.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            30225..30515
                     /gene="rplW"
                     /locus_tag="IWJ77_000429"
     CDS             30225..30515
                     /gene="rplW"
                     /locus_tag="IWJ77_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9452079.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            30554..31387
                     /gene="rplB"
                     /locus_tag="IWJ77_000430"
     CDS             30554..31387
                     /gene="rplB"
                     /locus_tag="IWJ77_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9452080.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            31430..31708
                     /gene="rpsS"
                     /locus_tag="IWJ77_000431"
     CDS             31430..31708
                     /gene="rpsS"
                     /locus_tag="IWJ77_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9452081.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            31731..32078
                     /gene="rplV"
                     /locus_tag="IWJ77_000432"
     CDS             31731..32078
                     /gene="rplV"
                     /locus_tag="IWJ77_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9452082.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            32092..32748
                     /gene="rpsC"
                     /locus_tag="IWJ77_000433"
     CDS             32092..32748
                     /gene="rpsC"
                     /locus_tag="IWJ77_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9452083.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            32751..33185
                     /gene="rplP"
                     /locus_tag="IWJ77_000434"
     CDS             32751..33185
                     /gene="rplP"
                     /locus_tag="IWJ77_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9452084.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            33175..33363
                     /gene="rpmC"
                     /locus_tag="IWJ77_000435"
     CDS             33175..33363
                     /gene="rpmC"
                     /locus_tag="IWJ77_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9452085.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            33388..33654
                     /gene="rpsQ"
                     /locus_tag="IWJ77_000436"
     CDS             33388..33654
                     /gene="rpsQ"
                     /locus_tag="IWJ77_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9452086.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            33712..34080
                     /gene="rplN"
                     /locus_tag="IWJ77_000437"
     CDS             33712..34080
                     /gene="rplN"
                     /locus_tag="IWJ77_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9452087.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            34117..34425
                     /gene="rplX"
                     /locus_tag="IWJ77_000438"
     CDS             34117..34425
                     /gene="rplX"
                     /locus_tag="IWJ77_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9452088.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            34453..34992
                     /gene="rplE"
                     /locus_tag="IWJ77_000439"
     CDS             34453..34992
                     /gene="rplE"
                     /locus_tag="IWJ77_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9452089.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            35011..35196
                     /locus_tag="IWJ77_000440"
     CDS             35011..35196
                     /locus_tag="IWJ77_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9452090.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            35232..35630
                     /gene="rpsH"
                     /locus_tag="IWJ77_000441"
     CDS             35232..35630
                     /gene="rpsH"
                     /locus_tag="IWJ77_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9452091.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            35662..36198
                     /gene="rplF"
                     /locus_tag="IWJ77_000442"
     CDS             35662..36198
                     /gene="rplF"
                     /locus_tag="IWJ77_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9452092.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            36359..36715
                     /gene="rplR"
                     /locus_tag="IWJ77_000443"
     CDS             36359..36715
                     /gene="rplR"
                     /locus_tag="IWJ77_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9452093.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            36736..37236
                     /gene="rpsE"
                     /locus_tag="IWJ77_000444"
     CDS             36736..37236
                     /gene="rpsE"
                     /locus_tag="IWJ77_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9452094.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            37251..37430
                     /gene="rpmD"
                     /locus_tag="IWJ77_000445"
     CDS             37251..37430
                     /gene="rpmD"
                     /locus_tag="IWJ77_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9452095.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            37474..37914
                     /gene="rplO"
                     /locus_tag="IWJ77_000446"
     CDS             37474..37914
                     /gene="rplO"
                     /locus_tag="IWJ77_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9452096.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            37914..39209
                     /gene="secY"
                     /locus_tag="IWJ77_000447"
     CDS             37914..39209
                     /gene="secY"
                     /locus_tag="IWJ77_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9452097.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            39271..39918
                     /locus_tag="IWJ77_000448"
     CDS             39271..39918
                     /locus_tag="IWJ77_000448"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9452098.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            40109..40327
                     /gene="infA"
                     /locus_tag="IWJ77_000449"
     CDS             40109..40327
                     /gene="infA"
                     /locus_tag="IWJ77_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9452099.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            40360..40476
                     /gene="rpmJ"
                     /locus_tag="IWJ77_000450"
     CDS             40360..40476
                     /gene="rpmJ"
                     /locus_tag="IWJ77_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9452100.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            40494..40859
                     /gene="rpsM"
                     /locus_tag="IWJ77_000451"
     CDS             40494..40859
                     /gene="rpsM"
                     /locus_tag="IWJ77_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9452101.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            40887..41276
                     /gene="rpsK"
                     /locus_tag="IWJ77_000452"
     CDS             40887..41276
                     /gene="rpsK"
                     /locus_tag="IWJ77_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9452102.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            41356..42294
                     /locus_tag="IWJ77_000453"
     CDS             41356..42294
                     /locus_tag="IWJ77_000453"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9452103.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            42322..42702
                     /gene="rplQ"
                     /locus_tag="IWJ77_000454"
     CDS             42322..42702
                     /gene="rplQ"
                     /locus_tag="IWJ77_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9452104.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            42974..43813
                     /locus_tag="IWJ77_000455"
     CDS             42974..43813
                     /locus_tag="IWJ77_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9452105.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            43789..44658
                     /locus_tag="IWJ77_000456"
     CDS             43789..44658
                     /locus_tag="IWJ77_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9452106.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            44648..45445
                     /locus_tag="IWJ77_000457"
     CDS             44648..45445
                     /locus_tag="IWJ77_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9452107.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            45648..46394
                     /gene="truA"
                     /locus_tag="IWJ77_000458"
     CDS             45648..46394
                     /gene="truA"
                     /locus_tag="IWJ77_000458"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9452108.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(46727..49396)
                     /locus_tag="IWJ77_000459"
     CDS             complement(46727..49396)
                     /locus_tag="IWJ77_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9452109.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(49634..49822)
                     /locus_tag="IWJ77_000460"
     CDS             complement(49634..49822)
                     /locus_tag="IWJ77_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9452110.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            50092..50454
                     /locus_tag="IWJ77_000461"
     CDS             50092..50454
                     /locus_tag="IWJ77_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9452111.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            50506..52188
                     /locus_tag="IWJ77_000462"
     CDS             50506..52188
                     /locus_tag="IWJ77_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9452112.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            52305..54341
                     /gene="recG"
                     /locus_tag="IWJ77_000463"
     CDS             52305..54341
                     /gene="recG"
                     /locus_tag="IWJ77_000463"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9452113.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            54390..55391
                     /gene="plsX"
                     /locus_tag="IWJ77_000464"
     CDS             54390..55391
                     /gene="plsX"
                     /locus_tag="IWJ77_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9452114.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            55513..55755
                     /gene="acpP"
                     /locus_tag="IWJ77_000465"
     CDS             55513..55755
                     /gene="acpP"
                     /locus_tag="IWJ77_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9452115.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            56023..57027
                     /locus_tag="IWJ77_000466"
     CDS             56023..57027
                     /locus_tag="IWJ77_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452116.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            57028..57972
                     /locus_tag="IWJ77_000467"
     CDS             57028..57972
                     /locus_tag="IWJ77_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452117.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            57972..58934
                     /locus_tag="IWJ77_000468"
     CDS             57972..58934
                     /locus_tag="IWJ77_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452118.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            58961..59875
                     /locus_tag="IWJ77_000469"
     CDS             58961..59875
                     /locus_tag="IWJ77_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452119.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            59901..61682
                     /locus_tag="IWJ77_000470"
     CDS             59901..61682
                     /locus_tag="IWJ77_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9452120.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            62030..62716
                     /locus_tag="IWJ77_000471"
     CDS             62030..62716
                     /locus_tag="IWJ77_000471"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9452121.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            62736..66317
                     /gene="smc"
                     /locus_tag="IWJ77_000472"
     CDS             62736..66317
                     /gene="smc"
                     /locus_tag="IWJ77_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9452122.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            66326..67135
                     /locus_tag="IWJ77_000473"
     CDS             66326..67135
                     /locus_tag="IWJ77_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9452123.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            67146..68144
                     /gene="ftsY"
                     /locus_tag="IWJ77_000474"
     CDS             67146..68144
                     /gene="ftsY"
                     /locus_tag="IWJ77_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9452124.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            68331..70598
                     /gene="gshAB"
                     /locus_tag="IWJ77_000475"
     CDS             68331..70598
                     /gene="gshAB"
                     /locus_tag="IWJ77_000475"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9452125.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(70687..71139)
                     /locus_tag="IWJ77_000476"
     CDS             complement(70687..71139)
                     /locus_tag="IWJ77_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9452126.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            71289..72239
                     /locus_tag="IWJ77_000477"
     CDS             71289..72239
                     /locus_tag="IWJ77_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9452127.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            72232..73191
                     /locus_tag="IWJ77_000478"
     CDS             72232..73191
                     /locus_tag="IWJ77_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9452128.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            73188..73943
                     /locus_tag="IWJ77_000479"
     CDS             73188..73943
                     /locus_tag="IWJ77_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9452129.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            73966..74919
                     /locus_tag="IWJ77_000480"
     CDS             73966..74919
                     /locus_tag="IWJ77_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9452130.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(75563..75859)
                     /locus_tag="IWJ77_000481"
     CDS             complement(75563..75859)
                     /locus_tag="IWJ77_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9452131.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            76214..76570
                     /locus_tag="IWJ77_000482"
     CDS             76214..76570
                     /locus_tag="IWJ77_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9452132.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            76580..77479
                     /locus_tag="IWJ77_000483"
     CDS             76580..77479
                     /locus_tag="IWJ77_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9452133.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(77876..78955)
                     /locus_tag="IWJ77_000484"
     CDS             complement(77876..78955)
                     /locus_tag="IWJ77_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9452134.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT        25553 a        14399 c        16914 g        22106 t
ORIGIN      
        1 ctttcatata catgaaaata gtagttatac ggatttttat cattctttat ccctttttgg
       61 gcaaatacct cattccattc ctcataattc acttctggaa aaaaagtatc tccttcgaat
      121 tcatgatgta tttttgtgat gtacattttc tcaacataag ggaaaaacaa attataaatc
      181 tgttctcctc cgaaaataaa aatttcttct tcgtttttac ataactcaaa aacatcttct
      241 attgaatgaa caatttcaca accattaaag gtaaacccct tatctctcgt cagaataata
      301 tttcttctgt caggtaaggc tcttccgatt gattcaaggt tcttcctacc taatattatc
      361 ggatgtccct ttgtagtatt tttaacatat tcccagtcct tgggaatcct ccaaggaatg
      421 tcattctctt tgccaatcac tctattctta tccatcgcag caatcaaaga aactttcatt
      481 tataacacag cctttttaac taatttacca tctaatttct tatttctatc aaaaaacctt
      541 ttctcattat cctgcccgtt agttgaataa gcaatttttt ctataagtta tattatcata
      601 ataccacaaa atcacaaatt ttcattcaat tatcattttc aattgcattt tataaatcat
      661 tttgtatgta atttggtatg taagttaaaa tgaaaaaagt agtccatttt gaagtacatt
      721 ttgtcatttt gtactccaaa actgaactac ttacttgcat atatacgcct ttatagtgga
      781 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      841 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      901 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
      961 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1021 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1081 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1141 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1201 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1261 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1321 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1381 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1441 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1501 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1561 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1621 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1681 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1741 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1801 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1861 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1921 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     1981 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2041 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2101 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2161 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2221 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2281 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2341 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2401 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2461 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2521 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2581 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2641 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2701 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2761 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2821 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2881 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     2941 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3001 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3061 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3121 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3181 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3241 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3301 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3361 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3421 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3481 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3541 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3601 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3661 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3721 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3781 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3841 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3901 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     3961 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4021 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4081 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4141 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4201 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4261 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4321 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4381 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4441 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4501 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4561 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4621 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4681 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4741 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4801 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4861 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4921 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     4981 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5041 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5101 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5161 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5221 aaggcgagcg ttcgtcatat tgtattagat aaatgcacat taacttattt atccgaatgg
     5281 aaggaagtac aacaaaatct aattcagaca gactttgtaa tgagctataa cggtattcct
     5341 acccaaaaac atacactatc atatgctatt gaacgctatg ctaagctggc tggtatccat
     5401 cgaatcagaa tccatgggtt acggcattct catgcttcct tgttaatcag tatgggagaa
     5461 aatccactta tcattaaaga tcgtctagga cacgaagata ttgagacgac tttaggaacg
     5521 tatggacatc tttatcctaa cagtaatttc gaggtagcta acaaattaaa tggagccatt
     5581 tcctttaaag aagcaaaaca aaattatgat acttcaccaa agaatcaatt tactgtagac
     5641 tacttgcgaa acaaagaaat gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga
     5701 gccagaaacc cttggtataa gaggatttct agctctccta caactattcc cactccactg
     5761 ttgctggtgg tttagaagtg atgtcataca cgatacggtt gacgtgggct acttcgttta
     5821 cgatacgtac agagattttt tgcaagactt cccatgggat acgggcaaag tcagcggtca
     5881 ttccgtcgat tgaagttaca gcacggatac cgactgtgta gtcgtaagta cgtccgtcac
     5941 ccataacacc gacagagcgg atccctggaa gaacagtgaa atattgccag atatcacggt
     6001 caagtccagc agctgcgatt tcttcacgca agatagcatc agattcgcgt acgatttcta
     6061 gcttgtcttc agtgatttcg ccaagaacac gaataccaag accaggacca gggaatggct
     6121 gacgccaaac gattgaatca ggcatgccta actgagtccc taattcacgg acttcgtctt
     6181 tgaataatgt gtttaatggt tcgatcaatt caaattgcat atcttctggc agtccaccta
     6241 cattatggtg agatttgatc gtctgtgctg tttctgtacc agattcgata acgtcagtat
     6301 acaacgttcc ttgtgctagg aaagagacgc cttcttcacc agcaagtttt gttgcttcgt
     6361 catcaaaaac atatacgaat tcattcccaa tgattttacg tttttgttct gggtcagata
     6421 cacctttcaa cttgtctaag aagcgatctc ttgcatcgac tttgatgatg ttcaagccga
     6481 attttccgcc tagactttcc attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt
     6541 caacaaagat agatgtcaat tggtcaccga tcgctttttg taaaagaacc ccaacgacac
     6601 tagaatcaac gccacctgaa agaccaagca agactttttt gtcaccaact tgttcacgga
     6661 ttttttctac ttgcatatcg ataaagttat ccattgtcca gttaccttca cagtgacaaa
     6721 catcaaaggc aaagtggcgc agcaattcga ttccgtattc ggaatgacgt acttctgcat
     6781 ggaattggat accataaaaa ttacgtgttg tgtcttggat agaagcaatc ggacaatctt
     6841 tgcttgttcc gactgtttca aagccttcag gaactttagt taccaagtca ccatggctca
     6901 tccatactgt ttgttgtttt ggtgtttctt taaacaagac agcttgatcg cttgttactt
     6961 ctaacaaggc tttcccatat tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt
     7021 acgtgatcaa ttgcatacca tagcaaatcc ctaatacagg aatacctaaa ttgaagatct
     7081 caggatcgat cccaaatgca ttctcatcat atacactgtt tggtccgcca gaaagaatga
     7141 ttccctttgg attcatcgct tttacttctt ctgcagtaat gcggtggctc aataattcgg
     7201 aaaagactcc aagatcacga atacgtcttg tgatcaattg gttatactga ctaccgtagt
     7261 ctaatacgat aatcttttca acgttcgtca tatcggcaac gtttgtcaaa tttattaccc
     7321 ctatcccttt aaattttatc tcaaaatagt caaactattt taaaatgcta atattttcgc
     7381 atgaaaatcg aatcgatgat atgattttca gttttatgga gaattatatc ggctcttcct
     7441 cgtgtaggca agatatattc ctgtaaattt ttcaaattca catttttcca tacatctttt
     7501 gccattttaa aggcttcttc acgatctccg atagcaaaac gataataata attctctgga
     7561 ttttggaaag ccgtatccag taacgcacca aaacgatcca aataccattt ttcgatcagc
     7621 acgggatcgg catcgacata gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga
     7681 ttcgcaggta attgaagcgt attgatcccc tcaacgatca agatatcagg ctgctggatc
     7741 aattcgtact tgtctggaat gatatcatac acatcatgtg agtaaacagg tacttttatc
     7801 tcttcctgac cagattttac acggttcaag aaatcgatca gcatctccat atcataactt
     7861 tcaggaaatc ctttacggtc catgattcca cgctctttta atatacgatt tggataaaga
     7921 aatccgtcag tcgtgatcag ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc
     7981 tgcagcagtc tggctgttgt actttttcca acggctacac ttccggcgat cccgataata
     8041 aaaggcggta cttctacgta tttatgaaga aataaacctt tactcaagaa caaagattca
     8101 aattctttca tataaagata aatcagatga gcaagaggta catagatatc tcgaacatcc
     8161 tgcatggaaa tgcggtcatt caaacttttg atattatcta gttcatcttg tgtcaaaggt
     8221 gcttgcccat tacgataaaa ttctcgccat tcttctcttg ccacacggta gtagttcatt
     8281 ggatcattca tattagcctc ctgcgttctc ccatcaatgg aactatttta acacaaaata
     8341 aacgcaaacc ccaccactta agagagattg ccgatctttt tgtataatct ttataataga
     8401 aagaaacgga gtcttattct tgacttgcgc acggatacat aaaaggatag actgttgtca
     8461 tgaaagaaat gagaaggatg gaagacatga caaatcatta ctattctgaa cgcccagata
     8521 cggcgcacga ttttgaacaa tgggcttttg agttaaaagg cagaaccttt caatttgtaa
     8581 cagattctgg tgtattttca cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg
     8641 ctttcgattg ggaagagtta cctgatgaag gaaagatctt agatgtggga tgcgggtatg
     8701 gaccgattgg gttagctttg gcatttgcga cacagcggtt tgttgaaatg gtcgacatta
     8761 atgaacgagc ggtaagtcta gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg
     8821 atatccatca atcgaatatc tatgaagcag ttcatgaaga aacctatgca gctatcgtaa
     8881 gcaatccgcc gatccgtgct ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc
     8941 ctcggttgaa gaagggcggt acactgacaa ttgtgatcca gaagaaacaa ggagcgccga
     9001 gtgcgcaaaa gaaaatggaa gaaacatttg gtaatgcaga aatcgtcaca aaagataaag
     9061 gatattatat catcaaaagt gtgaaagaat aactagctga aaaatagtgg agcgacgaaa
     9121 gagtggaact gggcgtgcat gtacgggtac gtatcagttc tttttttgtt gagaagtttt
     9181 taaaaacaaa gaactatttt ttcttcaaaa agaaatagta ggaacagagt cgttgttttt
     9241 tacgataaaa taaataagat acagaacttg tatagaaggc tgccaagtcg gtagataaag
     9301 gaggttttta tcaaaaaaca tactttcctt ttttctctat tcttttcttg ggggttttgg
     9361 tacactgata gggaatttag atagaaggga gccaatagga tgaagctgac catcagagat
     9421 atcgcagaaa tggcaggcgt atcagtaaca actgtctcac agattttgaa taacaaagga
     9481 agcagattta gtgataagac aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat
     9541 aagcccgatt atttcgcttc aaatgtgatc aatcgacatt caaagaccat cggaatgatc
     9601 gtccctgatg tcactgattt cttcttctcc aaactgatcg aaggtgtaga gagctatttg
     9661 aatcctttgg gctatgtgat catgttatgc aattctcgtc atagccaaga aaatgagatc
     9721 aagtatttac aagagctttc ccaccgctca gtggacggaa tcttgctggc cacaccaaat
     9781 attttacctg agaaatatgc attagacagc ggtttttatc aaaaaatgcc aatcatttta
     9841 atcgatcgcg gattgaatcg tcgggataac ggacggctta tcgtaaagga atatgagggc
     9901 gcctatcaag ctgtttcgct gtttattgaa aatggccata cgaagatcgg catgctgaag
     9961 gaaaccactg gatactatca attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa
    10021 gatcatggcc tgccttttaa tgggaaattt gttgaacatg gagagttgac tgtccaaggt
    10081 ggatatactg catcaaaaaa attactcaaa cataaagata tcacggctat tttttgcggg
    10141 aatgatgcca tggcgatcgg atgctatcag gcgatcgatg aattgggcaa aaaaatccca
    10201 gaagatatct cagtcatagg attcgatgga ttaaagctgt cagaatacat gattccgcaa
    10261 ttgacgacag tgcagcagcc aagttttgat attggatttt atgctgcacg tttcctgatc
    10321 gatacgatcg aatttcctca aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt
    10381 attcgtgaga gcgtaaaagt attgacaaag ggctaactcg gcggtatatt gcttatgtaa
    10441 gcatttacaa aggctagtta gtgctttttt tatttttagt aaaccagttt actaaagtgg
    10501 tttacctttc ctaaaaataa tgtataatac cgaatgaaga caaggaggat caggcaatgc
    10561 gaatggtgga tctgattgaa aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg
    10621 agtacatcgt cacgaactat acgaatggaa agattccaga ttatcaagtc agtgcattat
    10681 tgatggcaat tttttatcaa gacatgacaa atgaagaaat cacgaactta acattggcga
    10741 ttgctaattc tggtgatgtc atcgatttga gttctcttga aggaataaag gtagataagc
    10801 attcgacagg tggcgttggt gacacgacca cattgatttt agccccccta gtagctagtg
    10861 tcggagtgac agtggctaaa atgtctggac gcggtctagg ttacacaggt ggtacactag
    10921 acaagctaga agcaattccc ggatttcaga tcgagctttc ggatgaagaa tttgtacgca
    10981 ttgttaatga aagtaaagta gcggtaattg gtcaatcagg aaacttggca ccggcggaca
    11041 aaaaattgta tgccttgcgt gacgtcacag cgaccgttga ttctcttccg ctgattgcca
    11101 gttcgattat gagtaaaaaa atcgcggcag gagcagatgc aatcgttttg gatgtgacca
    11161 ctggagacgg tgctttcatg aagaacatcg aagatgcaag acgtctggca aagaccatga
    11221 cgagcattgg gaaactggct aaccgagaaa cagtggcggt tatctcagat atgtcagaac
    11281 ctcttggcga agctatcgga aatagtctag aagttgtcga ggcaatcgaa acattacaag
    11341 gaaatggtcc agaagatcta gtagaaatgt gctacgcctt aggaagccaa atggttgttc
    11401 ttgctggcaa agctaagaca accgatgaag caagaacgtt gcttcaagaa gctctggaat
    11461 caggtaaagc gttagcaaaa ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga
    11521 tcgtggaaca tccagaacga attttgactg cacgctacac aatggagctt cctgctaaac
    11581 aaagcggagt tgtgtcaaaa atcgtggcaa atgaattagg aattgctgca atgatgctag
    11641 gtgctgggcg taaaaccaaa gaagaagata ttgatcacgc tgttggattg aaactgcata
    11701 aaaaaattgg tgacactgtg acaaaaggtg agtcactatt gacgatctat agtaacgacg
    11761 aagaaatcac ttctgtcatt gaattattat ataaaaatat tgaaattggt gaatcggcaa
    11821 tgaagccaac gttgattcat gacatcatca cagaataaag gaaaaaagcg gactttaccg
    11881 ttttaaagaa aaggggaaaa gaaatggaac taaaccgaat gatcgaccat actattttga
    11941 aagcagacgc atctaaagaa gctgtgatgc aaattatcga agaagcaaaa aaataccatt
    12001 tttattctgt ctgcatcaat ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg
    12061 agccagtagc cgtatgtaca gtaatcgggt ttcctctagg agcaaacaca cccgagacaa
    12121 aagcatttga aacaacagat gccatcaata acggtgcaga tgaagtcgat atggtcatca
    12181 atatcggtgc tttgaaatca aaggatgatc aacaagtcca aaaagatatc gaagcagttg
    12241 ttgaagcagc aaaagatcgt gcgcttgtca aagttattat tgaaacatcg ttattagata
    12301 gagaagaaat catccgagct tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa
    12361 catcaactgg tttctcaaca ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa
    12421 ctgtaggacc agaaatgggt gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa
    12481 tggcaatgat cgaagctggt gctacaagaa tcggaacaag cgcaggagta gcaatcgtat
    12541 ctggttcaac tggtgaaggc tattaatttt ttcaaaggag aagctcatgg cagaactcaa
    12601 acaagaatgg atcaacacag ctattgaagc gttagataaa gcctatgttc cttattccca
    12661 ttttccggta ggcgcttgtc tcgtaacgga atcaggcaag atttatcaag gcatcaacat
    12721 tgagaatgca tcatttggat taacgaattg tgccgagcgc acagcttttt tcaaagcagt
    12781 atctgaagga gaaaaaacat ttacgcatct agtcgtagcg gggcacaccc ccgatcctat
    12841 ctctccttgt ggtgcatgtc gacaagtaat ggcagaattc tgttcccctg atatgccggt
    12901 tacgctagta ggagacaatg gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta
    12961 tgcatttact gaaaaagatc tataactcat gttcattcag tcagctgact gttgaaaaat
    13021 aaaacaatta ttttaggagg ctttacagaa atgaaaaaag caaaattatt tggtttaggt
    13081 gctgtcgcac tagcagcagg gttattttta ggtgcatgcg gcaacaacgg ctctactgat
    13141 tcatccggtg gcaaaagctc atcagacaca acaactgcag cattgatcac agacacaggt
    13201 ggtgtagacg accgttcatt caaccagtca gcttgggaag gactagaaaa atgggggaaa
    13261 gatcaaggac tttcacgagg aaatgatggc ttccaatatt tccaatcttc aaatgaatca
    13321 gattacatcc caaatattga ccaagcattg aatgctggat tcaaaacgat ttttggtatt
    13381 ggttataaac taaaaccagc gatccaagaa caagcaacaa acaatacagg tacgaacttt
    13441 gttattatcg atgatgtcat cgatggatta gataacgttg tatctgcaac tttcaaagac
    13501 aacgaagctt cttatcttgc aggtgttgca gcagcttata cgactgaaac aaacgtagtc
    13561 ggctttatcg gcggtgtaaa aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc
    13621 ggtgttgatg caggtgcgaa agaattagga aaagaaatca aagtattgaa tcaatatgct
    13681 ggtgacttct ctgctccaga taaaggacgt tcaattgctc aaggtatgta tgcacaaaac
    13741 gcagatatca tcttccatgc ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa
    13801 tcattgaacg aatcaggcga caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca
    13861 gacgaaggcg aatacacatt aaacggcgaa aagaaaaact tcacattgac atctacactg
    13921 aaagcagttg gaacagtagt agaagatttg gcacaaaaat cagcggacgg taaattccct
    13981 ggcggcgaac acactgttta tggattgaaa gaagacggtg ttggtctaac agaaggacaa
    14041 ctttctgacg aagcgaaaaa agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac
    14101 gtaaaagttc cagaaactcc tgaagaaaac taaaaccatt tctatagcct tgttagacac
    14161 atgtttgaca aggctatggt ttgattagaa caagaattgt tcatttatcc aagctgcctt
    14221 gtgacagttt ggataaggga acaactccct gaagtaatgt ttgtttcaaa agaagaacgc
    14281 tcactaagcg tgaatcttag tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa
    14341 aaaggtggtt caagaaaata ttgtcataga aatgcagaat attaccaaag aatttggaac
    14401 attcaaggca aatgacaaca tcaatttaca agtaaaggca ggagaaattc atgcgttgct
    14461 tggagaaaac ggtgctggca aatctacatt gatgaacgtg ctttccggat tattagagcc
    14521 gacatcaggg aaaattttga tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc
    14581 caaccaatta gggattggga tggtccatca gcactttatg cttgttgatg cctttactgt
    14641 aacagaaaac atcgtgttgg gaagcgagcc tagtcgcgca gggatgcttg accataaaaa
    14701 agcacgaaaa gagatccaaa aagtttctga acagtatgga ttgtcagtca acccagatgc
    14761 ttacgttcgt gatatttcag ttggaatgga acaacgggta gaaattttaa aaacacttta
    14821 ccgaggagca gatgtactga tttttgatga gccgacagct gtattgaccc ctcaagaaat
    14881 tgatgaatta atcgtgatca tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat
    14941 tacgcataag ttagatgaaa tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg
    15001 aaaaggaatc ggtacagtca acgttaaaga cgtaacctca caacaattag ccgatatgat
    15061 ggttggaaga acggtttcat tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt
    15121 tttgtctatt gaaaatctag tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt
    15181 gaacttagag gttcgtgctg gcgaagtact tggtatcgct ggaatcgatg gaaacgggca
    15241 gtcggagttg atccaagctt tgactggttt gcgaaaggca gaaagcggac atatcaagct
    15301 aaaaggggaa gacatcacta ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca
    15361 cgtgccagaa gaccgtcata aatacgggtt agtcctagat atgacattat ctgaaaatat
    15421 cgcactacaa acctatcatc aaaaacctta cagtaaaaac ggcatgctga attattcagt
    15481 aatgaatgaa catgccagag aattgatcga agaatatgat gttcgaacaa cgaatgaact
    15541 tgttcccgca aaagctttat caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt
    15601 cgaccgagat cctgatctgt tgattgttgc aaatccaact cgtggactgg atgtaggtgc
    15661 gatcgaattt attcataaac gtctgatcga acaaagggac aaatacaaag cagtgttact
    15721 gatcagtttc gaattagaag aaattttaaa tgtttcggat cgtattgctg ttatccatga
    15781 aggagaaatt gtcggtatcg ttgatccgaa agaaacatct gaaaatgaat taggtctatt
    15841 gatggcgggc tataccttag aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca
    15901 tgaatgatcg cagcgaaaaa ttccgtaatt tattagtgcc tgtactttca gttgttttag
    15961 gattgatctt aggcgcgata ttgatggctg cttttggttt tcagcccatc caaggttatt
    16021 cttctatgct agatgcagcc cttggttcgc aaagagggat aggtgaaacg ttaaggcaag
    16081 caacgccgtt gatttttaca gcattaggtt tttctgtagc taactcagct gggtttttca
    16141 atattgggct ttctggacaa gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg
    16201 cgtttccaga tctgccaaaa gttgttttgc ttccgttatg cgtgatctta ggtgccttag
    16261 ctggtgcgat ggcagcggct gttccaggtt tacttcgagc attttttgga acaagtgaag
    16321 tgatcgtaac aatcatgatg aactatatct tattatacgt aagcacattt gctttacatg
    16381 aagtgatgcc tgaaagctat cgctcaagtt tggactcttc caatatgatc agtcaaaatg
    16441 cctcactgcg gttaccatgg ttaacacaag cttttggcgg ctctcgcgta aactcaggat
    16501 tatttttagc aatcatcgcg cttattttcg tttggctgct gatgaagaaa acgactttag
    16561 gatttgaaat ccgttctgtt ggattgaatc cttttgcttc tgagtatgca ggaatgagta
    16621 gtaaacgtac gatcgttttg tcgatggtca tttctggcgc tttggcaggg cttggcggtg
    16681 tggttgaagg attaggaacg tatcaaaact tctttgttca aacgacttcg ctatctattg
    16741 gatttgacgg aatggctgtt tccttgcttg gttctggatc agcaatcggt attcttttat
    16801 cagcgatctt gttcagtatt ttgaagatcg gtggacttgg catgcagaca ggagcaggtg
    16861 ttccgtttga gatcgtcaat gtatcgattg ctttgattat cttctttgtc gggatcaact
    16921 tcctgattcg tttcgtcatg gctaaagtct tccaagggaa aaaacaagaa gagatcgttg
    16981 cgacaataga atcgaaacca acgaatcaaa accatgaagg gggcgaacta taatgtcgac
    17041 tatcgaatta attgccttga tccttacttc tacactggtt tattcaacac cgctaattct
    17101 aacgtcacta ggcggaactt tttctgaacg aagcgggatc gtaaacgtag ggctagaggg
    17161 aatcatggtc atgggtgctt ttagttccat cgttttcaac ttgacgatga gtccatcact
    17221 tggttctatg acaccatgga tcgctatttt agtgggtggc gtcgtaggta tcctattttc
    17281 tctgattcac gcagtagcaa cagtcagttt gcgtgctgac catattatca gcgggacagt
    17341 aattaatctg atggcacctg ctttaggtgt tttcttgatc aaagcatggt atggtaaagg
    17401 tcaaactgat aacatcacgc agaatctggg gtatttttct ttcccaggat tagcagatat
    17461 tccggtcatt ggaccaattt tctttaaaaa tacattctta cctgcttatt gtgctatttt
    17521 gattgctgtc ctagcttggg tgatcatctt caagacgaaa ttcggcttgc gcctccgttc
    17581 agttggtgaa aatcctcaag ctgccgacac attaggtatc aatgtatatg ctatgcgtta
    17641 ttcaggcgta ctgatttctg gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc
    17701 gatttccggt aacttttcag ctggtacgat cgttggacaa gggttcatct ccatggctgc
    17761 tatgatcttt ggtaagtgga atcctttagg ggcaatgggt gcctcattgt tctttggttt
    17821 tgctcaaagc ttgagtatca ttggcgcaca attaccagtt atttcttcga ttccgccagt
    17881 attgctgcaa attgctccat atttgctgac aatcattgtg ctggttgttt tcttagggaa
    17941 agcttctggt ccaaaagcaa acgggaagaa ctatatcaaa tcaaaataat cttttagcga
    18001 ataaaaaggg tgggttacgg gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat
    18061 aaaaataaaa agaaagaagg aataatcatg tttaaacgcg tacatctgat cgtcatggat
    18121 tcagtaggaa ttggagaagc accagatgcc gataagttcg gtgatgttgg ttctgataca
    18181 ttaggacaca ttgcgaaaga agcaggattg aacgttccac atttagagca gcttggttta
    18241 ggaacgatcc gtcctcttga aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg
    18301 aaattagaag aagtttctgt agggaaagat acgatgacag gccactggga aatcatggga
    18361 ttgaatatca aaactccatt tcgtgtgttt cctaatggat tccctgatga tctgatcaaa
    18421 caaatcgaag aattttctgg ccgtaaagtc gtatgtaaca aaccttatag cggaacagct
    18481 gttattgacg attacggtga acaccaaatg aaaacaggcg atttaattgt atatacttct
    18541 gccgatcctg tccttcagat cgcggcacat gaagaaatca ttcctttaga agaattatat
    18601 cggatttgcc aattcgttcg tgatataaca aaagatgatc cgtatatgat tggccgaatc
    18661 attgcacgtc cgtacgtggg tgaaccagga gacttcacac gaacaagcaa tcgccatgat
    18721 tatgcgttga atccattcgg acacacagtc cttgattctt tgaaagaagc aggaaaggac
    18781 gtgattgctg ttggaaagat caacgatatt ttcaacggac aaggaatcac tgaatctgtc
    18841 cgcacgaaaa gcaatatgga tggcgttgac cagctgctga aagttatgaa aaacgatttt
    18901 tcaggaatca gctttaccaa tttagtagat tttgatgcat tatacggtca tcgtcgggat
    18961 gtagtcggct atgcgcatgc gattgaagaa tttgacttac gaattccaga attgcttgaa
    19021 gcgatggaag aagacgactt gctaatgatt acagccgatc acggaaacga tccgacgttt
    19081 actggtaccg atcatacgcg agaatatgta ccgttattgg tttacagtaa aaaaatgaaa
    19141 gaacaagggg cattgcctca aggatattat tcagatattt cagcaacgat tgctgagaac
    19201 tttagtgttt cttcgactga aaatggcgaa agcttcttga aattattaaa ataatctttt
    19261 taaataggag aaagcaaatg accaagttaa gcgagatgtt acaagaaacg acccagttta
    19321 tccttgataa aggagtaaaa gaaattgatt ttggtttgat tctaggttca ggattaggtg
    19381 aattagcaga tgaaatcgaa gatgctgtaa tgatttctta tgatgagatc ccgttctttc
    19441 ctgtttcgac agtagttggg catgcaggaa agctagttta tgggtctctg gcaggcaaga
    19501 aagtgttagc catgcaaggg agattccact actatgaagg tcattccatg caaacagtga
    19561 cctatccagt acgtgtgatg gccggactag gggcccattc agtcattgtg actaatgcat
    19621 gtggtggtgt caacgaatca tttgtaccag gagatttgat gctgattacc gatcatatca
    19681 attttactgg tgaaaatcct ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc
    19741 cagatatgag tcaagcttat acgcctgact atcaagcaac agcaaaagca gcagcagaaa
    19801 aactaggttt ggatcttaaa gaaggcgtat atatgggcta ttctggacca acttatgaaa
    19861 cgcctgcgga gatccgaatg tctcggacaa taggagccga tgcagtaggg atgtcgactg
    19921 ttccagaagt catcgtagca gctcacagcg gattaaaagt gttaggaatc tcttgtatta
    19981 ccaacttagc agcaggtatg caagcgaatc tgagccatga agaagttgta gaaacaactc
    20041 agagagtcaa acaatctttt aaagcattgg ttaaagaaac attgacatta ttgtaatagg
    20101 aggaacaata aaatgagtgt gcatattgaa gcgaaagaag gcgagatcgc cgataagatt
    20161 ttacttccgg gagatccttt gcgagctaaa tatattgcgg agactttttt ggaagatcca
    20221 gtttgttaca atcaggtacg cggtatgtta ggctttaccg gcaaatataa aggagaacgt
    20281 atttctgttc aaggaacagg aatgggaatg ccatcagcca ccatctatgc ccatgaattg
    20341 atccagtcgt atggtgtaaa gaaattgatt cgagtaggta catgcggtgc attgtcaaaa
    20401 gacgtccatg ttcgtgattt agtactagca caaggtgcag cgaccagttc ttcgatgatc
    20461 gaaaaaaatt tccaagcctt tcattttcct ccaatcagtg atttcaatct attactgaaa
    20521 gcatatgaga tagctaaaga aaagggctat acggttcatg taggaaatgt tctttcggaa
    20581 gattctttct acaaagatga tttgacagag acgttccaat tagctgaact tggcgtttta
    20641 ggagtggaaa tggaagcagc ggctctttat tacttaggcg cgaaatacca tgtgcagaca
    20701 ttaagtttga tgaccgttag cgaccatttg atcaccggtg aagaaacaac agctgcagaa
    20761 cgtcaatcta cgttcaatga aatgatcgaa gtaggattgg aaacagcgat agcagaataa
    20821 gaaggaaatg aaaaagctgc cctgcattaa gtaataagaa catccaagca aaaatagctc
    20881 ctcatgtttt tgacttggct ggacttatta ttgcaaatgt aagcttttga acaccgttta
    20941 ccaagaggtt gtgaatctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag
    21001 cttttcatgt ttgatgaata ttgtgacttg tgaccgagaa gttggcagga gaaaaccgtt
    21061 tatacgaaaa aagggactgt gacaaatcgt gtcacagtcc cttttttata gtgtttttta
    21121 taggtagtat tttttagcaa ctgttaagtc atcatttaat tcataaacaa gtggttgacc
    21181 tgttggaatt tcaagatcca tgatgtcttc gtcagagata ccttcgatat gtttagctag
    21241 ggcgcgtaat gagttaccat gtgcagctac taaaacagtt ttgttgtcta gtaatgctgg
    21301 agcaatttca tcttgccaga atggcaaagc gcgttctaat gtcactttca agttttctcc
    21361 accaggaata tcgcgtttgt ctaacattgc gtaacgacgg tcatttgctg cagaaccttc
    21421 gtcagtttct tccattaatg gaggaagagt atcgtaagaa cgacgccaga tgtgcacttg
    21481 ttcgtcgccg tatttttcag ctgtttcttt tttgttcaaa ccttgtaatt taccatagtg
    21541 acgttcgttt aagcgccatg atttgatttg tggtacccaa agttgatctg aatattctaa
    21601 gatcaagtta catgttttga ttgcacgagt taaaacagaa gtgtaagcaa catcaaattc
    21661 gattccagct tctttgattt tgcgtccgcc ttcttttgct tcttcgattc cttctggtgc
    21721 tagatttaca tcagcccaac cagtaaattg gttcaatgca ttccattcac taagaccgtg
    21781 acgagaaaaa actaattttg gcatgtttag ctctcctttt aagataggtt tatatttctg
    21841 aaattttcag ttctgtttta ttgtacaatt ttttggaaga gatttccatc gaaaaattag
    21901 ataagattct atgagaaaac aagtagagaa tataaaaaaa cgaacagtgc ccacgcaact
    21961 gttcgttttt aggtattaga tgcataattg ctcgatttct ttttcaggta aattgatata
    22021 tttataacat gttccaactg aaacaccgca ttttgaagaa ataaattgga tcgtttcttt
    22081 tttttcataa tagagacgtc ggattttttt gatcgttttt gcatcgattt ttggtcttcc
    22141 gccgattttt ccttttttac gagcttcatc taatccaact aatgtacgtt ctttgatcaa
    22201 tgcgcactcc atgtttgcta ggttttccat taattggaaa tagatttttc ccatttcgcg
    22261 cgtatcgatt ccttcatcaa gactaatcaa gtgcagcccg cgatctttga ataattgcgt
    22321 gagttctgtt aattgccgcg tagatttgcc taatcgatga agttggcaaa tgactagtgc
    22381 atcgccttgt tgcatgtcag cgatcactga ctccaaatga acagcagtag aatcattttg
    22441 ttccacttca tatgtctcat ggaaaatttc ttgacaacca aagtcagata attgatcaac
    22501 ctgagttgtt aaatcaacgt catttatggt tgtgcgtgca taaccaataa ttttcatggt
    22561 gattcctttc tagcagtgat tttctaactg gatataacag taaaaatgta gcataaagta
    22621 cgtcaaaaag atagtttttt cgcaaatttt ccgctgtctt tttttattgg atgatcaagc
    22681 agtttttctt tatttacgac ggattgcgtc aagaatctca acgccttttt ctgagccgac
    22741 aactacttta gaaaccatgt ttaggaaaag cccatgttct actaccccaa caagttcatc
    22801 taaataggta gctaagcttt ctggctgttc gatgacttct agatgcagat caatgatgta
    22861 atgaccacca tctgtggtta agacttctcc gttttcgtct gtacgcaaga cgggttggaa
    22921 tcttttttct tcaaaaatat gaagtagctg ttgggagcca taagggatca cttcaactgg
    22981 gagtgggaac ttcccaagtt tatcgactaa tttagaagaa tcaacgatcc aaatcgtttc
    23041 ttttgagtac gtagcgacta ttttctcgaa aagcaaagca gcgccgccgc ctttgatccc
    23101 ttgaaaatca gcactgattt catcagcacc gtctattgtc aaatcgacta cggggacttc
    23161 atcaatactc tttaacggta ttcctaattt ttccgcttgt tcttttgtcg catttgaagt
    23221 agtaactcct gtgatacgta aaccttcttc acgcatacgg cgaccaatct cttctaccat
    23281 ataataggcg gttgaaccgg tcccgagacc tacgatcatc ccgtccttta cgaatttcgc
    23341 ggcttcaatg ccagctaatt gttttaaatt catctttgcg tccacctttt tttagtttga
    23401 gcatcgtgct ttcctcacta ttttagcgtg atttacggta aaaagatacc attctttttt
    23461 attccaataa agctttttca aaaaaaattg catctcttgt tgacatgttt tttcattcgt
    23521 gatattctaa caaaggtgct ttatctacag gtttctgtga agaaacgtgc tgactgttta
    23581 tcaaagcata tttgataagg atgcagacat tgcatttatt ttttatcaca aaaaaagaac
    23641 cacctggatg tgtggaacta gacgcaaatc gtgggaagga ggaaacaagg aatgcctaca
    23701 attaaccaat tagtacgtaa acctcgtaaa tcaaaggtgg aaaaatctaa ttcacctgct
    23761 ttgaacaaag gatataacag ctttaagaaa actcaaacta acgtaaactc acctcaaaaa
    23821 cgtggggtag caactcgtgt gggtacaatg acacctaaaa aaccgaactc agctttacgt
    23881 aaatatgcac gtgttcgttt gtcaaactta attgaagtta ctgcttatat cccagggatt
    23941 ggtcataact tacaagaaca cagtgttgta ttattacgcg gtggtcgtgt aaaagactta
    24001 ccaggggtac gttaccatat cgttcgcggt gctttagata ctgctggtgt taacgaccgt
    24061 aaacaaagcc gttctaaata cggaactaaa aaacctaaag cttaatttta attattaaac
    24121 aaatgaaact tgcttctact atatagaagt aattactatg aacaatattt cggaaggagg
    24181 agttacggat gccacgtaaa ggtcctgttg caaaacgtga tgttttacca gatcctattt
    24241 ataactcaaa attagtaact cgcttgatca accgtgtaat ggttgacgga aaacgcggga
    24301 ttgcagctaa cattatctat aatgcctttg acttgatcaa agaatctaca ggtaacgatc
    24361 cattagaagt gtttgaacaa gcaatgaaaa acgttcaacc tgttcttgaa gttaaagcac
    24421 gtcgtgttgg gggttcaaac taccaagtac cagttgaagt tcgccctgaa cgccgcgtta
    24481 ctctaggtct tcgttgggta gtaaactacg ctcgcttgcg cggtgaacac acaatggaag
    24541 aacgcttagc aaaagaaatc atggatgctg caaacaacac aggtgcttct gttaaaaaac
    24601 gtgaagacac tcataaaatg gctgatgcca accgtgcgtt tgctcactat cgctggtaag
    24661 attctctctg tttggtcggc ttttataagt cgacctcaga tagtgtcctt atttttagca
    24721 actagatagt aaagagagga gaaacaagga gaatgaccag agaattttct ttacaaaaca
    24781 ctcgtaatat cggtatcatg gcccacatcg atgctggtaa aacgactgct actgaacgta
    24841 tcttgtacta tactggtaaa atccataaaa ttggtgaaac tcatgaagga gcttcacaaa
    24901 tggactggat ggaacaagaa caagaacgtg gtattacgat cacttctgct gctactacag
    24961 cacaatggaa gggtcaccga atcaacatca tcgacacacc aggacacgta gacttcacag
    25021 ttgaagttga acgttcattg cgtgtacttg atggtgctgt aactgttctt gatgcacaat
    25081 caggtgttga acctcaaact gaaacagttt ggcgtcaagc aacaacttat ggtgtacctc
    25141 gtatcgtatt tgcaaacaaa atggacaaga tcggtgctga cttcttatat tcagtatcaa
    25201 ctttacatga ccgtttgcaa gcaaacgctc acccaatcca attaccaatt ggtgcggaag
    25261 acgacttcac aggaattatc gaccttgtaa caatgaaggc tgaaatgtat acaaacgatc
    25321 ttggaacaga gatcgaagaa acagaaattc ctgaagaata ccgcgaatta gctgaagaat
    25381 ggcgcgaaaa attggttgaa gctgtagctg aaactgatga agaacttact ttgaaatact
    25441 tagaaggtga agaaatcact gaagctgagt taaaagaagg tatccgccgc gcaacagtta
    25501 acgtagaatt ctacccagta ctatgtggtt cagcatttaa gaacaaaggg gttcaattac
    25561 tattggacgc tgttcttgat tacttgccat caccgttaga tatcccagct atcaaaggga
    25621 ttgaccctaa aactgatgaa gaagtagaac gtcctgcaga cgacagcgct cctttctcag
    25681 ctttagcatt taaagttatg actgacccat tcgtaggtcg tttaactttc ttccgtgtgt
    25741 actctggtgt tttacaatca ggttcatacg tacaaaacgc aacaaaaggc aaacgtgaac
    25801 gtgttggtcg tatcctacag atgcatgcca actctcgttc agaaatctct gaagtttacg
    25861 ctggagatat cgctgctgct gttggtttga aagacacaac tactggggac acactatgtg
    25921 atgaaaagag tctagttatt cttgaatcca tggaattccc agaaccagtt atccaagtcg
    25981 ctattgaacc taaatcaaaa gctgaccaag ataaaatggg tgtggcttta caaaaactat
    26041 ctgaagaaga tcctactttc cgtgctgaaa caaacgtgga aacaggcgaa acaatcatcg
    26101 ctggtatggg tgaacttcac ttagatatca tcgttgaccg tatgcgtcgt gaattcaaag
    26161 ttgaagctaa cgtaggtgcg cctcaagtat cttaccgtga aactttccgt gctggcactc
    26221 aagccgaagg taagtttgta cgtcagtctg gtggtaaagg tcaatacggt cacgtatgga
    26281 ttgaatttac accaaatgaa gaaggtgctg gtttcgaatt tgaaaatgcg atcgtcggtg
    26341 gggttgttcc tcgtgaatac attccagctg ttgaaactgg tttgaaagat gctatggaaa
    26401 atggtgtctt agctggctat ccattagtag atatcaaagc taaactttac gatggttcat
    26461 accatgatgt cgattcaaat gaaactgcct tccgtgttgc tgcttctatg gcattacgtg
    26521 cagcagctaa gaaagctaat ccagtaatcc ttgaaccaat catggctgta gaagttgtta
    26581 tccctgaaga ttatttaggt gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgttg
    26641 aaggtatgga agctcgcgct ggcggccaac aagttgttcg tgcgatggtt ccacttgctg
    26701 aaatgtttgg ttatgctaca actcttcgtt ctgctactca aggtcgtggt acatttacaa
    26761 tgacatttga ccactacgaa gatgtaccaa aatcagtaca agaagaaatc attaagaaaa
    26821 acggcggaaa agctgaataa tttcttaaaa ccggatagtc ctgcaaagag attatccaaa
    26881 gagactatat ttttaatata gtttcgtgta aaataattat cgatgatatg ggcatgtggt
    26941 gtaccttgtg tacatcatat acctatcaga caaataaaaa atatattgta tttttaggag
    27001 gacattttaa aatggcaaaa gaaaaatttg accgttctaa accacacgtt aacattggta
    27061 ctatcggaca cgttgaccat ggtaaaacta cattaacagc tgcaatcaca actgtactat
    27121 ctaagaaaaa cggcggccaa gctatggctt acgatcaaat cgatggtgct ccagaagaac
    27181 gcgaacgcgg aatcacaatc tcaacagctc acgttgagta tgaaacagat acacgccact
    27241 acgctcacgt tgactgccca ggacacgcgg attacgttaa aaacatgatc actggtgctg
    27301 ctcaaatgga cggagctatc ttggtagttt ctgctgctga cggcccaatg cctcaaactc
    27361 gtgaacacat cctattgtct cgtcaagttg gtgttcctta catcgttgta ttcttgaaca
    27421 aagtagacat ggttgatgac gaagaattac tagaattagt tgaaatggaa gttcgtgacc
    27481 tattaacaga atacgaattc cctggtgacg atgttcctgt agttgctgga tcagctttga
    27541 aagctctaga aggcgacgct tcatacgaag aaaaaattct tgaattaatg gctgcagttg
    27601 acgaatacat cccaactcca gaacgtgaca acgacaaacc attcatgatg ccagttgaag
    27661 acgtgttctc aattactgga cgtggtactg ttgctacagg tcgtgttgaa cgtggacaag
    27721 ttcgcgttgg tgacgaagtt gaagttgttg gtattgctga agaaacttca aaaacaacag
    27781 ttactggtgt tgaaatgttc cgtaaattgt tagactacgc tgaagctgga gacaacattg
    27841 gtgctttact acgtggtgtt gcacgtgaag acatccaacg tggacaagtt ttagctaaac
    27901 caggtacaat cacacctcat acaaaattct ctgcagaagt atacgtgttg acaaaagaag
    27961 aaggtggacg tcatactcca ttcttcacta actaccgtcc acaattctac ttccgtacaa
    28021 ctgacgtaac aggtgttgtt gaattaccag aaggaactga aatggttatg cctggcgaca
    28081 acgtaacaat ggaagttgaa ttaatccacc caatcgctat cgaaaacggt actaaattct
    28141 caatccgtga aggcggacgt acagttggtg ccggtgtcgt tacagaaatc aaagcttaat
    28201 tcacttaagt tttaaagtaa agcaacattt attcggacgt aatcgctcgg aacgaaggtc
    28261 ttaggatctt tgttccgagc ttttttattt attgcaggat caggaaaaaa gacaggagta
    28321 ttataaatag aagaaacacg aaataaaaga gtcagtattt tctaaatgaa actttatgaa
    28381 aacaaaatgt tttttacaag aaaaagcctt gctttgtgag tttatatata ttataatgac
    28441 aaaggtgcgt ttactttagt gaattgcaca gacgtaatca gaatgatttc gtcggctaag
    28501 aaacgagagg ttgcgacacg cccggacgct ttgccacgaa cgagcgtgac ggaaattttc
    28561 gtggagctat gtctactttt caaaataggc gaaggaggga acaagatggc aaaacaaaaa
    28621 attcgtatcc gtttaaaagc gtatgaacac cgtattttag atcaatcagc ggataaaatc
    28681 gtggaaactg caaaaagaac tggagctagc gtgtctggtc caattccatt accaactgaa
    28741 cgcagtcttt acacaatcat ccgtgcgact cataaataca aagattcacg cgaacaattc
    28801 gaaatgcgta cacacaaacg tcttatcgac attgtgaacc caacaccaaa aactgttgat
    28861 gctttgatga agctagactt accatctggt gtaaacatcg aaatcaaatt ataaaactaa
    28921 aacaaaaata atggaggtgt actcatgacc aaaggaatct tagggaaaaa agtgggaatg
    28981 acacaaatct tcactgaatc tggtgaatta attccagtaa ctgttgtaga agctactcca
    29041 aacgtagtac tacaagtgaa aacaatggaa acggatggct acgaagctat tcaagttggt
    29101 taccaagata tgcgtgaagt tttatcaaac aaacctgcga aaggtcatgt tgcaaaagca
    29161 aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg ttgagctagg agaatatgaa
    29221 gtaggaaaag aaattacagt agatgttttc caagcaggag acattattga tgtaacaggt
    29281 actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc acggacaaag ccgcggacca
    29341 atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa tgggtccagt tgcacctaac
    29401 cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg gcaaccgcgt aactatccaa
    29461 aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta tcttgatcaa aggtaatgtt
    29521 cctggagcta aaaaatcttt aattactatc aaatcagctg tgaaagctaa ataattgtag
    29581 gaagaaagga ggaactaagg aatgccgaat gtagcattat tcaaacaaga tggaagccaa
    29641 aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg aacctaatga aagtgttgtt
    29701 tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag gaacacacgc ggttaaaaac
    29761 cgtagcgctg ttcgcggcgg cggacgtaaa ccatggcgtc aaaaaggaac aggtcgtgca
    29821 cgtcaaggat ctatccgctc accacaatgg cgtggaggtg gcgtagtctt tggaccaaca
    29881 ccacgttctt acagctacaa acttcctaaa aaagttcgtc gtttagcaat gaaatctgta
    29941 ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg aaggcttgaa cttcgacgca
    30001 ccaaaaacaa aagaattcaa acaagttctt gctaacttat ctatcgactc taaagtattg
    30061 gttgttctag aaaaaggtaa cgactttgct gctttatcag cacgtaactt accaaacgtt
    30121 tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg tatcaaatac aaaagtgtta
    30181 gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg cataatgaac ttactagacg
    30241 taatcaaacg cccagtgatc actgaaaaat caatgcttgc catggatgac aagaaataca
    30301 cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa acaagcggta gaagctgctt
    30361 ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg tgcgaaattc aaacgtatgg
    30421 gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat cgttacattg actgaagatt
    30481 caaaagaaat ccaattattc gaagctgctg aataagcact aatagataac tcattaaatt
    30541 aggagggaaa aacgtggcga ttaaaaagta caaacctacc tcaaatggtc gtcgtaacat
    30601 gacaggttct gattttgctg aaatcacaac atcaacacct gaaaaatcat tgttacaacc
    30661 attgaaaaac catgctggac gtaacaacaa cggccgcatc actgtacgtc atcaaggtgg
    30721 cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt aacaaagata acgttgttgc
    30781 aactgttcaa acgatcgaat atgatccaaa ccgttcagct aacatcgcgt tagtacacta
    30841 tgaagatgga gtgaaagctt acatcttagc acctaaagga ctagaagtgg gtatgaaact
    30901 tgtttcaggt ccagaagcag atatcaaagt cggaaatgct cttccattag aaaacattcc
    30961 agttggtact gttatccaca acattgaaat gaaacctggt aaaggtggac aattgatccg
    31021 ttctgctggt acaagtgctc aagtacttgg taaagaaggc aaatacgtat tgatccgctt
    31081 gaactctggt gaagttcgta tgatcttagc tgcatgccgt gctacaatcg gttctgtagg
    31141 taacgaacaa cacgaattaa tcaacatcgg taaagctgga cgctctcgtt ggatgcgtaa
    31201 acgcccaact gtacgtggta gcgtaatgaa ccctaatgat cacccacacg gtggtggtga
    31261 aggtaaagct cctatcggac gtaaagcacc agtttctcct tggggtcaac cagctcttgg
    31321 ttacaaaact cgtaacaaaa aagctaaatc agacaaactt attgttcgtc gtcgcaaaaa
    31381 taaataatta ctttgaccat gtgtcgcaca ttttgagagg aggttcacca tgggtcgtag
    31441 tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa aaagttgaag ctcaagcagg
    31501 ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt tcaacagttt tcccaagttt
    31561 tgttggatat accatcgcag tttatgatgg acgtaaacat gtaccagttt acatccaaga
    31621 agacatggta ggacacaaat taggtgaatt tgcacctaca agaacttatc gtggacatgc
    31681 tgctgacgat aagaaaacga aacgctaatt tgaggggagg ataaatccaa atggcagaac
    31741 aaatcacatc agctaaagca actgcaaaaa cagttcgcac ttcacctcga aaagcccgtt
    31801 tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc aatttcaatc ttgaaattca
    31861 caccaaacaa atctgctgga atcattgaaa aagtattgat gtcagcagtt gcgaatgcag
    31921 aaaataactt tgacttagat gttgaaaact tggtagtaag cgaagctttt gttaacgaag
    31981 gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc agcttctcca atcaacaaac
    32041 gtacaagtca tatcacggta gtcgtatcag aaaaataagg agggataatc agtgggtcaa
    32101 aaagtacatc caattggaat gcgtgtaggc atcatccgcg actgggatgc taaatggtat
    32161 gctgaaaaag agtatgctga gttcttacac gaagatttaa gaatccgtaa atttatttca
    32221 actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg aacgcgcaac aggtcgcgta
    32281 aacatttcaa ttcacacagc taaaccaggt atggttattg gtaaaggcgg ttctgaagtc
    32341 gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa gaatccatat caatatcgtg
    32401 gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg aaggaatcgc acgtcaatta
    32461 gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca tccaacgctc aatgcgtgct
    32521 ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa acggagcaga tatcgctcgt
    32581 tcagaaggat actcagaagg aactgttcct cttcatactt tacgtgcgga cattgattac
    32641 gcatgggaag aagcagatac aacatacgga aaactaggag ttaaagtgtg gatctatcgt
    32701 ggagaaattc ttccaactaa aaaaaacact gagaaaggag ggaaataatc atgttagtac
    32761 ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat gcgcggggaa gctaaaggcg
    32821 gaaaagaagt agcattcggt gaatacggtt tgcaagctgt tgattcacat tggatcacaa
    32881 accgccaaat cgaagctgct cgtatcgcaa tgactcgtta catgaaacgt ggtgggaaag
    32941 tatggattaa aattttccct cacaaatctt atactgccaa agcaattggg gtacgtatgg
    33001 gttctggtaa aggggcacct gaaggatggg ttgcaccagt aaaacgtggt aaaatcatgt
    33061 ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc gttacgtcta gcttctcaca
    33121 aattaccaat gaaaactaag atcgtaaaac gtgaggaaat gggtggtgaa tcgaatgaag
    33181 gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg atcaagaaaa acaattaaaa
    33241 gaagaattgt ttaatcttag attccaatta gcaacaggtc aattagaaaa cactgcacgt
    33301 attaaagaag tacgtaaatc gattgcacgc atcaaaactg tgttgcgtga acaagctaag
    33361 taattatgga aggaggccat taagcgtatg actgaaggaa gaaatcaacg caaagtttat
    33421 caaggtcgcg tggtttcaga caaaatggat aaaacaatta ccgttgctgt cgaaacaaag
    33481 aaaaaccacc ctatctatgg caaacgcatg aactattcta agaaatacaa agtgcatgat
    33541 gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta tggaaactcg tccattatca
    33601 gctacaaaac gtttccgttt gttggaagta gtcgaagaag cagttattat ctaataacac
    33661 gagattttcc tatatagatt gaatgcaaaa tctgaaagga ggatacacaa cgtgatccaa
    33721 gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac gtgaaatttt gacgatcaaa
    33781 gtccttggtg gatctggtcg taaaactgct aacatcggtg atgtgatcgt tgctacggtt
    33841 aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg tcgttaaagc cgttatcgtt
    33901 cgaacaaaat caggagctcg tcgtacagac ggttcatata ttaaatttga tgaaaatgct
    33961 gcagtgatta ttcgtgatga taaaagccct cgtggaacac gtatctttgg gccagttgca
    34021 cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac tagcaccaga agttttataa
    34081 tcttgagaca cttatcaaag gaggtgcgaa acagttatgt ttgttaaaaa aggcgataaa
    34141 gttaaagtta tcactggaaa agacaaaaac aaagaaggcg ttgtactagc agcgttcccg
    34201 aaaaaagata aagttattgt cgaaggtgta aatgtcgtta aaaaacacca aaaaccttct
    34261 caagctgctc ctcaaggtgg aattgttgag atggaagcgc caattcatgt ttctaacgtg
    34321 atggtggttg actctactgg tgtagctggc cgagttggct acaaagaagt agacggcaaa
    34381 aaagtccgtg tttctaaaaa aaccggtgaa gttttagata aataaaagtt taaggaagga
    34441 gggtcttact gaatgaaccg cctaaaagaa aaatatctta aagaagtaac tccatcattg
    34501 atggaaaaat ttaactatag ctctgttatg caaacaccta aagttgaaaa aatcgtcatc
    34561 aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact tagataaagc agttgaagaa
    34621 ttaactttga tcactggtca aaaaccaatg attacaaaag ctaaaaaatc aatcgctgga
    34681 ttccgtttac gtgaaggaat gccaattggt gcaaaagtta cattgcgcgg agaaagaatg
    34741 tacgaattct tagacaaatt ggtttctgtt tcactaccac gtgtacgtga cttccatggt
    34801 gtaagtaaaa aagcctttga cggtcgtgga aactatactt taggtattaa agaacaattg
    34861 atcttcccag aagtagatta tgatttagta gataaagtac gtggtatgga catcgttatt
    34921 gtaacaacag caaactctga tgaagaatct cgtgagttgt tgacacaatt aggcatgcca
    34981 ttccaaaaat aattaaagga ggcgaactac gtggctaaaa aatcaatgat tgctaaaaac
    35041 aaacgtcctg caaaattttc aacacaagct tatactcgtt gcgaacgttg cggacgtcca
    35101 cattcagttt atcgtaagtt ccatctttgc cgtatttgct tccgcgaact tgcctataaa
    35161 ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag taaattcgca taagaaggag
    35221 gtaaaagatc aatggtcatg acagatccaa ttgcagattt tctaactcgt atccgtaacg
    35281 caaacatggt taaacatgag tctttagaag tacctgcatc aaaaattaaa cgtgacatcg
    35341 ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga atacatcgaa gatgacaaac
    35401 aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg cgaacgtgtt atcacaaact
    35461 taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa agctgacgaa gtaccaaaag
    35521 tattgaacgg tttaggtatc gcaatcattt cgacttctga aggtgttgtc actgataaag
    35581 aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta cgtatggtaa tcaagtagaa
    35641 tctacaagga ggtgtcttta agtgagccgt atcggaaata aagtcgttgt tatccctgaa
    35701 ggagtaacag ttactcaaga cggaaacaac gttacagtta aaggaccaaa aggtgaattg
    35761 actcgtactt tttcagcaga tatcaaaatg aatatcgaag gaaacgaagt aactttcact
    35821 cgtccaaacg acagtaaaga aatgaaaaca attcatggaa caactcgtgc gaacttcaac
    35881 aacatggttg taggtgtcag cgaaggtttc caaaaagcgt tagaactaat cggggttggg
    35941 taccgtgcac aattacaagg atcaaaactt gtattgaacg ttggttactc acatccagta
    36001 gaaatcacac caccagctgg agtaactgtt gaagtaccag ctaacacaca agtaattgtt
    36061 aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa acattcgtgg aactcgtcct
    36121 ccagaacctt ataaaggtaa aggtattcgt tatgttggcg aatatgtacg ccgtaaagaa
    36181 ggtaaaactg gtaaataatc gtttagaaaa attgcataga agccagcagc aggaaaaagg
    36241 catttggctg aagtttacct tcaaaaaaat acatttttac ttgtgccgca gcttctattt
    36301 ttcgaaacgt atttactgct tgaaatagta gtacaataaa aataaagagg tgacaattgt
    36361 gattacaaaa ccagataaga acaaaacacg tcaaaagaga catcgtcgtg tacgtaacaa
    36421 tatctctggt actgctgagc gcccacgctt gaacgttttt cgttctaaca aaaacatcta
    36481 cgctcaaatt attgatgacg tagcgggtgt aacgctagca agtgcctcta ccttggataa
    36541 agaaatttca ggtggaacaa aaacagaagc agcacaagct gtcggtaaac tagttgctga
    36601 acgtgcagct gaaaaaggca ttaaagtagt agtctttgac cgtggtggat acctttacca
    36661 tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat ggactagaat tttagaagaa
    36721 ggaggaatac cattcatggt ttatatcgat ccaaaacatt tggaattaga agaccgcgtt
    36781 gttgcaatta accgtgtaac aaaagttgtt aaaggtggac gtcgtctacg ttttgctgct
    36841 ttagttgttg tcggtgacaa aaacggacac gtagggtttg gtacaggtaa agctcaagaa
    36901 gttcctgaag caatccgtaa agcaattgaa gatgcgaaga aaaacttagt tgaagtacct
    36961 atggttggtt caacaatccc acacgaagta atcggtgctt ttagtggtgg tagaatccta
    37021 atgaaacctg ctgtagaagg ttctggggtt gctgctggtg gaccagttcg tgccgtatta
    37081 gaattagctg gggtagcaga tatcacttct aaatctttag gctcaaatac accaatcaac
    37141 gttgttcgcg caacagttga aggtttgaaa caattgaaac gtgcagaaga agttgcagaa
    37201 cttcgcggca aatcagttga agaattaatc ggataaggag gacaacaata atggctgaat
    37261 taaaagttac tttaaaacgc agtattatcg gacgtcctca aaaccaacgc gatacagtta
    37321 aagcgttagg tcttacaaag attaacagtt cagtggttaa gccttctaat gaagcaatta
    37381 aaggcatgat caatactgtt tctcacctag tggacgttga agaaatttaa tttagaaaaa
    37441 tcataactag aacattaagg aggtgccaaa cctatgaaac ttcatgaatt aaaacctgct
    37501 gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta cttcatctgg taacggaaaa
    37561 actgccggac gcggacaaaa aggacaaaaa gctcgttcag gcggcggtgt gcgtttagga
    37621 tttgaagggg gacaaacacc attgttccgt cgtttaccaa aacgaggatt cacaaacgtg
    37681 aaccgtaaag actatgcagt cattaatctt gatgtattga accgctttga agacggagct
    37741 gaagtaactc ctgttgcttt agttgaagct ggaatcgtga aaaacgaaaa agctggaatc
    37801 aaagtattag ctaatgggga actaaacaag aaattaactg tgaaagcagc aaaattctct
    37861 aaagcagctc aagaagcaat tgaagcggct ggtggatcaa tcgaggtgat ctaatgttta
    37921 gactattaaa agatgctttt aaagtaaaag acattagatc gaaaatccta tttactgtat
    37981 ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt accaggcgta aatgctgctt
    38041 cattgcaaaa cctttcttct ttgccatttt tcaacatgtt gaacttggta agtggtagtg
    38101 cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc atacattact gcttctatcg
    38161 tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt tgaatggtcg aaacaagggg
    38221 aagttggtcg taaaaaactg aatcaagcga ctcgatatct gacattagta ttggcattta
    38281 tccaatctgt cggtatcact gctggattca atcaatacgc tcaattaggt ttcgtgaata
    38341 atcctaacgt agcaacttac gtaacgatcg gtttgattct aacagccggt acgatgtttg
    38401 ttacttggct aggagaacag atcacagaaa aaggtatcgg aaatggcgta tcaatgatca
    38461 tttttgcagg tatcatttct agattgcctg aaagtatcag agaaatttat gaagattact
    38521 ttataaatat tgatccttca gacatatgga aatctgctat ttttgtggcc attttactag
    38581 tagcggtctt gttgatcgtg acattcgtaa catttttcca acaagctgaa cgcaagatcc
    38641 cgatccaata tacgaaacgc gtagctggcg cgcctacaag cagctatctg ccgttgaaag
    38701 taaatgcagc tggagttatt ccagttatct ttgctagttc gtttattgct acgccaaatg
    38761 ctgttttaca agcattgagc ggtagattcg gttctgaaaa ctggtacaaa gtgatgatgg
    38821 agatctttaa ctataataca gcacctggtg ctacgattta tacgattctg atcgttgcct
    38881 tcactttctt ctacgccttt gttcaagtaa accctgagaa attagcggaa aacttgcaaa
    38941 agcaaggaag ttatatacca agcgtacgcc ctggtaaggg gactgaagag tatgtctcaa
    39001 gattactgat gcgtttaagt acagttggtg cagtcttcct tggtttagtg gcattgcttc
    39061 caatcattgc tcaaatggtt tgggatcttc cacaatcaat cggattaggc ggtacaagct
    39121 tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca gttagaaggc ttaatgctga
    39181 aacgtaaata tgttggattc attgagtaag aaagcgtgtt gaggtttccc ctcaacacac
    39241 gttctcacac atttattagg agggaaaaca atgaacctca ttttaatggg gctgccaggc
    39301 gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt atggtattcc gcacatctca
    39361 acaggagata tgttccgcgc agcgatgcaa aacgaaacag ctcttggcct ggaagcaaag
    39421 tcttatatgg ataaaggtgc attagttcct gatgaagtaa caaatggaat cgtgaaagag
    39481 cgactagctg aaccagatac agagaaaggc ttcttattag atggctttcc acgtacgcta
    39541 gatcaagcaa aagctctgga cgcaatgcta aaagatttga ataaaaaaat tgatgctgtc
    39601 atcgatatcc atgttggtga agaaatcttg gtcgaacgtc tagccggccg atttatctgc
    39661 agcaattgtg gagcaactta ccataaaatt ttcaacccta caaaagttga agacacatgt
    39721 gatcgttgcg gtgggcatga attctatcaa agagaagatg ataaacctga gacggttaaa
    39781 aatcgtctgg ctatcaacat caaaaatagt gaaccaatct tggcttatta taaagaacaa
    39841 ggtttgctaa acactattga tggtgctcgt gaaatcgatg ctgtatttgc agatgtacaa
    39901 aaaatcattg aaaaatagca cgattgcccg tagccgactt gcattatgca gttactcttg
    39961 tcaagttcaa taaattatga tagaatatgt cagtccgact tctagagaat cctctagatg
    40021 atccattttg agaattttga ggtgggaact gcagtttccg ccattttatc gtgtgaaaat
    40081 gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa gatatgattg aagtcgaagg
    40141 tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt gaactagaaa acggacacca
    40201 agttcttgct actgtttcag gtaaaattcg tatgcattac atccgtatct tacctggtga
    40261 caaagtaacg gttgaattgt ctccatacga tcttaaccgt ggccgtatta cttatcgctt
    40321 taaataattg tactccgtaa aactacagga ggtattatca tgaaagtaag accatcagta
    40381 aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag gacgcgttat ggtgatttgc
    40441 ccagcaaatc caaaacataa acaacgtcaa ggataattgg aggtgctata aatatggctc
    40501 gtattgcagg agtagatatt cctcgtgata aacgtgtagt agtttcactt acttatatct
    40561 atggtattgg taacactact gctaaaaaga tcttagctga tgctggtgta tctgaagatg
    40621 ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat ccgtgcagaa gtagataaat
    40681 taaaagttga aggggatctt cgtcgtgaag tgaacttgaa catcaaacgt ttgatggaaa
    40741 tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc tgttcgtgga caaaacacga
    40801 aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat cgctggcaag aaaaaataat
    40861 tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa agtgaatcgt aaacgccgtg
    40921 tgaagaagaa tatagaagca ggtattgcac atatccattc tacattcaat aacactatca
    40981 tcatgatcac tgatactcac ggaaatgcat tagcgtggtc atcagctggt tcattaggtt
    41041 tcaaaggaag caaaaaatca actccttttg ccgctcaaat ggcagcagaa actgctacaa
    41101 aagcagcaat ggaacacgga ttaaagactg ttgaagtaac agttaaagga cctggttctg
    41161 gacgtgaagc agcaattcgt tcattacaag ctacaggttt agaagtgact gcaattcgtg
    41221 acgtgactcc agttcctcat aatggtgccc gccctccaaa acgccgtcgt gtttaatgag
    41281 tgccgctcat tttgagtttt atcagtaacg tcattgaaca agacactttt cgttttgaaa
    41341 ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa tcgcaaaaat tgatgaagaa
    41401 aaagattatg gcaagttcat cgttgaacct cttgaaagag ggtatgggac tactttaggg
    41461 aattccctac gtcgtatttt attatcttct ttgccaggtg ctgccatcac taatattcaa
    41521 atcgatggtg tactgcatga attctctacc atcaaaggtg ttagagaaga cgtcactcaa
    41581 atcatcttga acattaaagg cttagcgctg aaaatgtatg gccaagaaga aaaaaccctt
    41641 gaaatcgata ttaccggacc tgctacagta actgctggcg atattatcgt tgatagtgat
    41701 gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct ctgaaggagc tacgttccat
    41761 gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag cagatgaaaa taaaaaagaa
    41821 gatatgccaa ttggcgttct tccagtcgat tcaatctaca cacctgttcg tcgtgtgaac
    41881 tatcaagttg agaacacacg tgttggacac cgtgaagatt ttgataaatt gacgatggag
    41941 atttggacag atggttcaat tgaaccgttg gaagcaatga gtttgtctgc gaaaatcatg
    42001 actgaacatt tggatatttt tgtgaactta actgatgaag caaaaaatgc tgaaatcatg
    42061 gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa tgacgatcga agagttagac
    42121 ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa tcaatactgt acaagaactt
    42181 acaaataaat ctgagcctga aatgatcaaa gtccgtaact taggacgtaa atcattagaa
    42241 gaagttaaag caaaattgca cgacttaggt ttaggtttac gtaaagaaga ttaaaacctt
    42301 ttacgaagga gggaaaccaa cgtgagttat cgtaaactag gacgcacatc tagccaacgt
    42361 aaagcgatgt tgcgtgattt aacaactgat ttattaatca acgaacgtat tgtgactact
    42421 gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa tgatcacttt aggtaaacgc
    42481 ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac gtaatgaagt tgccagcgtg
    42541 cgtgaagaaa atgaggacat cgtcattgaa tcagctttac aaaaactgtt caacgatatt
    42601 gcacctcgtt acgcagaacg tcaaggcgga tacacacgca ttttgaagac agaaccaaga
    42661 cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct aaaatcacac taataacatc
    42721 actttataga tgattctttt agagtgttat gatgttggaa gatatttttc cgagtctagc
    42781 tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag cactcatcat aaagtgtttt
    42841 tttatacctt caattacctg tcatgacaga tatgtttgaa ggcataaaaa aacattttaa
    42901 gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt tagtaggaac tttaggatga
    42961 agaaggagta atcatggagc caattattga attagaaaag ataaattata aataccagcc
    43021 agatgatctt cgccccgcat tgaaagatgt ctcctttaca attgataaag gtgagtggat
    43081 cgctattatt ggacacaatg gttcaggaaa atcgacactt gccaaaacga tcaatggatt
    43141 gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa atattggatg aagaaaatat
    43201 ttggacgatc cgacagatgg ttggaatgat ttttcaaaat ccagacaatc agtttgttgg
    43261 ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat caagggattc ccagagaaga
    43321 aatgctagtg cgtgtgaaag atgcacttga gaaagttaga atggctgagt ttgcttcacg
    43381 cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt gccattgccg gcgtggttgc
    43441 cttacgacct gatatcatta ttttagatga agcaaccagt atgctagatc cagaaggtcg
    43501 tgaagaagtg atctctacga tcaagaagat caaagaagaa agtcaattga ccgttatatc
    43561 tatcactcat gatattgatg aagcagctaa tgccaatcgg atactagtga tgagacaagg
    43621 cgaacttgtc cgtgaaggta caccaaaaga aattttttct gctggtcctg aactgatcga
    43681 cttaggtcta gatctaccat ttcctgaaaa actgaagagc gcactgaaag aacgtggggt
    43741 tgatgtgccc agtgaatata tgacagaaga aaggatggtg gattggttat ggacatccgt
    43801 tttgaacaag tagacttcac ttatcagccg aatacaccat ttgaacaaag agccttattt
    43861 gacatcaata tgacgatcaa agaaaacagt tatactgcat tagtagggca taccggaagc
    43921 gggaaatcaa ccttacttca gcatttgaat gcgcttgtta agccaacaag cggtacagtc
    43981 catattggag aacgagatat tcagccggat acggataata agaatttaaa gcccatccga
    44041 aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc tattcgaaga aacggtagca
    44101 aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg aagaagaagc actagtccta
    44161 gcaaaagaaa cattagaaca agttgggctg gatgaaagct atttggaacg ttcgccattt
    44221 gaactttcag gagggcaaat gcgtagagta gcaatcgctg gtgtgcttgc catgaggccg
    44281 gaagtacttg tattggatga accaacggca ggacttgatc cccagggcag aaaagaaatg
    44341 atggagatgt tttggcgtct tcataaggaa cagcagatca ctattgtatt agttacgcat
    44401 ttaatggatg atgtagccaa ttttgcagat tatgtctatg tgttggaaaa gggacgaatc
    44461 gttaatagcg gggagccgca agaggtcttt caaaacattg aatggctaaa agaaaaacaa
    44521 ctaggcgtac ctactgccac cgagtttgca gaagaattaa tggctaaagg gatgaacttt
    44581 gctaccttgc ctttgacagc tgaagaatta gcagacgcta tcgtccatta tgctggagga
    44641 aatgcccatg atgaataaat tgatatttgg tcgttatatg cctggagatt ccttgatcca
    44701 tcgattggat ccccgagcta aattattagc cagtttttat tttatcggaa tcattttttt
    44761 agcaaataat tgggaaacgt accttttgtt ggcatttttc acattacttt ctgtcttctt
    44821 gtcaaaagta gatttgaaat tttttatccg aggcattcgc ccattgatct ggctaatcct
    44881 cttcactgta gctttgcagg tcttgtttac aagtggcgga gaaacttatt ggagttgggg
    44941 tgttttccat atcactgaat ttggtgtgat gaacggcctc tttattttct gtcgattcgt
    45001 gttgatcatt tttatgtcga ctctactcac gcttactaca ccgcctttgg aattatcgga
    45061 tgcgattgag tacttgcttc gtccattgaa agccgtacgg ttcccagtcc acgaaatttc
    45121 attgatgctg tcgattgctc ttcgttttgt gcctacattg atggacgaaa cagaaaagat
    45181 catgaacgct cagcgcgctc gcggtgttga ttttggtgaa ggaaacctag ttcagaaaat
    45241 gaaagcagtc attcctttgc tgattccttt atttgtcagc agcttcaacc gtgcggaaga
    45301 tcttgcgact gctatggaag caagaggcta caaaggtggg gaaggaagaa cgaaatatcg
    45361 tgtattgcat tggcataata gagacacgat ggttatcatt gcttttgtat tattaactgt
    45421 gctactgatc tttttaagag gataaacgtt cagtgggccg ctatctattc ttttcttgct
    45481 gatctgtttg caagaaagga tgcaagcagt gtatgacaag aaatattagg gtctgtggca
    45541 gactaaggtc acaggcctct ttcttttatc tgaaaagaaa gaacgaaaaa attttataga
    45601 aaaatcatta ttccagataa ccttttagga acaaaggaga gaaaaacatg gtacgttaca
    45661 aagcaatcat tgcgtacgac ggaacaaatt ttaatggatt ccaaaaacag ccaaatgggc
    45721 gtactgttca agaagaagta gaaaaaacat tacaaaaaat ggcaaatgga aaagaaatca
    45781 cggtctttgg ttcgggaagg acagatgccg gagtacatgc gatcgggcaa gtaatccatt
    45841 ttgattatcc agaggagcgt ccgttagaac gaatgcgttt cgcgctagat acccagtcac
    45901 cagaggatat tgctgtaaga caagtagaaa ttgtttcaga agacttccac gcaagatatt
    45961 tggtcaaaga aaagacgtat caatttcgag tggatatagg gaaaccacgc agtccttttc
    46021 gacgccatta tgcaagttat tttccttacc cacttgattt ggaaaaaatt cagcgggcgc
    46081 ttcctgattt attagggact cacgatttca cctctttttg tgcatcagga agctcgatag
    46141 aggataaaat acgtacgatc tacgaggcta agatggaggt aaatgaaaca aaagacgaat
    46201 tgttgtttac atttagggga aatggcttct tgtataagat gatacggatc ttagtaggaa
    46261 cgcttttgaa aattggcaat ggccgattgc ctgaagatag tatcccagat attattgcga
    46321 aaaaagatcg taatgcagca ggacctacag cacaccctga aggactttat ttgtatgaag
    46381 tgaaatatga ataagaggtt atgatagaag tggtcagctt caagaaataa gaaggtattt
    46441 acgaaaatta cttctcaaaa ttttgcaaaa tttcggctta ttttcgaaga agctacttct
    46501 ggaacaccgt ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc acaaagagca
    46561 atttcaaaaa acagcttctc atctttgatg aatatcgtga cttgtggacg aggcgttgat
    46621 aagtgaatca tctttttgtt acctattttt tctgtaacca aaaaacgatg gaacgttgtt
    46681 ttgattgccc ttggtgcgga cagtctgaac aggttccatc gtttctttat tttctacgtc
    46741 ttcttctgtg tgtgtttcgt ggttgttctt ctattgctgg atcaatcgtg ccataaaagg
    46801 ggcgattgcg gtcaaggtac cagacataaa gaataaacaa tgcaatacat aggacaaaaa
    46861 ggattgcacc tatcgtaaat ccttcaggca agtaactgaa agtgacagta tgtttacctg
    46921 ctggaacttt tatcatcaag aatgcgtctt tgaaattgct gaccttgact ggtttcccat
    46981 caattttagc tttccatcct ttgtcataag gaatagttgt caccaacatg gaatcttcag
    47041 cggtatttac agtcccagaa gcttttctgc cagtggcttc catttcgaca cctttttctt
    47101 tgatctgatt cattgcggct tgataggcta caacatctag accaacgact ttaggctcca
    47161 tgaaacttat ttctttcgtc ccataaaaac ttgcagtaaa tgtcactgtt gtctcttgag
    47221 gataatagcc aagatcatag tattgaccag aaatgttgat ttgcgttttt cgcgatgtac
    47281 cattgacagt gatcgtcgca ctagaacttt ctaattgcgc aaaattggtt gggaacaaac
    47341 ttaagtaagc ttgtgtattt gcaggcacat ggacttccca agtgagctct tttggctggt
    47401 tatctgctgt ttctgagtaa gtgacgccag tcgttgtctt tgtgatcgtc gtattcaact
    47461 gattcttcaa agtaggtgta tagaatgtaa aatactgttc atttagacca gacaatgtat
    47521 tgatcaagtt tgtctgactg gtcaaattat ctgtcactgg ctgctcaaca tcattaacgg
    47581 acaacggtgc agtaaatgct agtggcaatg cattgacatt ctgatacagt gtatagtcgc
    47641 cgctttgacc agcttggcgg aagccatatt tactgaacgt gctttcgttt ttagaaatgt
    47701 tatatttgat tcctgtaaac ccatccatca gcaaagtatt attgttataa cggatattca
    47761 aatttgtccc tcttgaacgg aaacctaatt tatccaggta agaggaagaa ttacgatttc
    47821 ttatagaaga aaacaaactg ataccactgt aaccatagtt gatcgaatcg tttgcagata
    47881 ctggatccag attttccaag cggtaaaacg agtcggaatc tttttttgtt tcatttacaa
    47941 gagctttcaa gtcaggataa ggatcagtat ataaactgcg ggaagcataa ttccaatcat
    48001 caagaatgcc atggatcata ctgttcgtat tgaccagtgc ttcagcagac atgatcaata
    48061 ataacaatat actcagatga ttgaatgaaa gttttttatg ctgataaaat cccacagcaa
    48121 aagcataaat ccctaagaaa accactgtca atacaaagga agtcatcgtc acataggtgt
    48181 agcttccttc aggtttgatg ccccatgcca ccgcaaagag agcagctacg accaaaatga
    48241 ttcctaacaa cagcccacca tcgtcttgtt ctatcttttc ccatcctttt cctgcaagca
    48301 ggagcactaa gaaagagaac agataggcat aacggaataa aaacatattc ggtgcatgca
    48361 taccatgcca gaacaaattc aatggaacaa gataaaaact tgcgataagt atggcgaaaa
    48421 gactgccgta caaaaattta tttttcaacg gtactttccg actggcaaaa taaaacagac
    48481 agaggactaa tggtagcaga cctgcgtaaa cgaacggaat cgatccgtat tttgtcgtgt
    48541 cataaacacc aatcatattt ttcatgatta gatccagata agaagtcgct tctgttttca
    48601 acgtagtgat ctgactcagt gcctcgccat ttgaccgaag atccagcact gctggcaata
    48661 caatgatcat tgaagcgccg cctgccagta aagaagtgat accataagga acaatcgttt
    48721 ttttatattc tgaccagttt gtcaatagac gggcgataaa atataagaaa gaaaatacgc
    48781 caatcataaa acccatataa aaactggata aaaacaagag taaataactg ataaataaaa
    48841 gtttcggccg tttaagatcc ataacacgat gaatccccaa aatcactaat ggaagataaa
    48901 caaatgcatc cagccacatg atgatctccg aatgtgcagt gataaatgag atcaatgcgt
    48961 aactgacgct aagtgcaaca tgcccccagc gaggaatctt gaacgtacgt gcaagaaacc
    49021 aaaaagccaa tccggcacag ccgaccttaa gcaaagtcag aacatacagt gcatctggca
    49081 tcatctggtt tggaaaaaag aacaccagtg gcgtgaagat cccgcctaga tagtaagaaa
    49141 ttaaagataa ataattcaat cctagagaag cattccatgt ataaaaaata ctttgctttc
    49201 caagaagaac atttcggaag ctggcatgaa aattagaaaa ctgtgaaaag gcatcacttg
    49261 ccaaaatact tctgctgctt ccaggataga ttccgatact tagataaaca agtgctaaaa
    49321 tcaaaaacgg aatcaggaaa ctagctgctg cgtagcgtcc gttttctcta aagaatgact
    49381 ttaaattttt tttcatattt attttccacc aaatttctat gaagttgtat tcagtttatc
    49441 atatccttgt ttttctctgc ttaaaaagag tctagttttc acaaaaaaat aaaatttaga
    49501 ataaataact tcataatttg ttcgtataac aaaaataaac gcaaaaaaag ggtaggaatc
    49561 aaaagtccca cccttaaaaa tttttattaa ataaaaaacc gttcactatc tgaacggtcc
    49621 aagtatcatt caattaaacg cgttcaactt ttcctgattt caaagcacga gttgacaccc
    49681 aaactttttt aggttttccg tcgaccataa cgcgaacttt ttgtaagtta ggtttaacag
    49741 tacgttttgt tgagttcata gcgtgtgaac ggttgtttcc actttttgtc ttgcgacctg
    49801 taaagtaaca aacttttgcc atggtaattt cctcctttgc cttgatttgg agcaatagaa
    49861 taattcagct cataccagac taacatatca tatagaatgg gaatttgcaa gtacattttc
    49921 atagatacaa ttgacaattg agaagaataa ttgtaaatta aacaaggtta tacctgaaat
    49981 tttgcaaggt ttttgagtac tttgggtaac tgccttttat ttcaagttgc gctatgataa
    50041 aatatgatag aagaaaaatg ggagtttttt acccgttaag gaggctcatt catggctgta
    50101 aaaattcaaa cgtcagctgg aaccattgaa attaccaatg atgtaattgc aacagttgtt
    50161 ggtggcgccg cgacagatgt gttcggtgtt gtaggaatgg ccagcaaaaa tcaaattaaa
    50221 gataatatta ctgagatttt gcgtaaagaa aattactcaa gaggagtagt tgtccgccaa
    50281 gaagataatg gtattgctgt agatgtttat acgattatca gctatggtac gaaaatctct
    50341 gaagtttccc gcaacgtcca agaaaaagtg aaatacaatc tagaggcatt attaggcgta
    50401 actgccaatt cagttaatgt ctttgtacaa ggtgttcgtg tactgccaga ctaggcagga
    50461 gactggctgg taactcagca gtcgatctga aggaggatta tctaggtgag tgtaacagaa
    50521 ataagtgcaa gccagttcca ggaaatggtc caggcaggtg cgaatcgtct gcaagtaaat
    50581 gcagaatacg tcaattcatt aaatgtattc cctgtgccag atggcgatac gggaacaaat
    50641 atgaatctat ctatgactag tggagctaag gcagtagtag attcaacctc agaaaaagta
    50701 ggagaattag cagcaatgct ttcaaaagga ttgctgatgg gtgcgcgcgg aaattccggc
    50761 gttattttgt ctcaattgtt tcgtggattt tctaagcaaa ttccagatgt tgtctcatta
    50821 aatgcttctg atcttgctgc agccttcact catggagttg aaactgcgta taaagcagta
    50881 atgaaaccag tagaaggaac gattttgaca gtagctagag aagcagcgaa agctggagaa
    50941 aagaaagcaa aaagtactga tgatgtcgtt gaagtaatga cagctgttgt aaaaggagca
    51001 aagcgtgctt tagctaaaac tccagacttg cttccagtcc taaaagaagt cggtgtcgta
    51061 gacagtggag ggcaaggact gctgtttgtt tatgagggct ttttaagtgc attgaatgga
    51121 gaattccaag cggatgaaac atatgaacct tctccagcag aaatggatga tatggtcaat
    51181 gcagaacatc atcgcagtgt ccaaggtcaa atggcgacag aagatatcca ttttggttac
    51241 tgtacagaaa tcatggtaaa aatcggagaa ggacctacag tcgatagtac attcgattac
    51301 gaagaattcc gtaactattt agatggtata ggagattctt tacttgtggt caatgatgac
    51361 gagatcatca aagtccacgt gcatacagaa aacccaggag aagtcttgaa ttatggacaa
    51421 aaattcggtt cattagtcaa aatcaaagtc gacaacatgc gcttgcagca tgagacgatt
    51481 ttggaacacg atgaagaagt gaacgagttt gcgaaaaaag aaataccacg tatcccgtat
    51541 ggtatcgtag cgatcgctgc tggtaaaggc gtccaagagc tgtttgaaag tttaggcgct
    51601 aattatgtta tcagtggtgg acaaacaatg aatccaagta cggaagacat tgttaaagcg
    51661 attcaagaag taaacgcaga taaagtgatt gtcttgccaa acaataagaa catcttcatg
    51721 gcagctgatc aagcagcaga agtagcagat atcccagtag cagttgttcc ttcaagaacg
    51781 atttcccaag gattgacagc tatgctttca ttcaatgatc aagcttcttt agaagaaaac
    51841 aaagaaacga tgacagaaat gttgtctagc gttgtgagcg gtcaagtcac tcacgcgatc
    51901 agagatacct caattgacgg tgtagagatc aaagaaggag actttttggg catgatcgat
    51961 ggaaaaatcc tgatctctaa cccagatatc acagcaacag cgttaagtac actagaacaa
    52021 atgatcaatg aggacacaga gatcgtaacg atcttggcag gtgatgaagg aacagttgaa
    52081 caagcaaatg aatttgctga gaaactaaca gaaaaacatc ctgatttgga aatcgagatc
    52141 caccaaggag atcagcctgt ctatccttat ctattttctg ctgaataatt tctgtgtcag
    52201 aatatctttc taacacagta ttcattcata aaaggccttc ggttagtgat caggtatgat
    52261 cgctgaccaa gtcctttttt tgttaaagga gggaggaaaa atttatgcgt tctttagatg
    52321 atccaatcac tatgctggca ggagtcggag aaaaaagagc agctagtctt gccagcttgg
    52381 gcatccaaac gattgaagac ttactgactt attatccttt tcgctacgaa gatattcagg
    52441 aaagaaatct aaacgagatc caggatcaag aaaaggttac tttgaaagga atcgtagtct
    52501 cgcctcctgt gatgaatcga tttggttata aaaaaagccg attgcaattt cgtatgatgc
    52561 aagaacatga tgtgttcaac gtttcttttt ttaatcaacc ttatctaaaa gaaaaagtca
    52621 ttttatcgga ggaaatcgcc gtatacggta aatgggatgc aaagcgtaaa gccttgaacg
    52681 ggatgaagat tttaggttct caaacaatgg atgacttttc gccgatatat cacgtcaaca
    52741 aatcaattcg tcaaacgaca ttagttgatc taatcaaaaa agggtttcaa gaatacggca
    52801 cattgattga agaaaatctg ccaaacaacc ttgtggagaa atatcgactg cttgaccgag
    52861 cttctgctgt aagaagtatg cattttccaa aagaccacga agaaaaccat caagccaaac
    52921 gacgtgtcgt ttttgaagag ttctttttat ttcaaatgcg tcttcaagga ttgaaaaaag
    52981 cagaaaaagc agaaacgaat ggtttggaga tcctttatga tgtccaaaaa ttgaaacaat
    53041 ttactcaaaa gctacctttt gaactgacaa atgcacaaaa acgtgtaacg aatgaaatct
    53101 gtcgggatct tcgcagtccg cagcatatgc agcgactttt gcagggagat gtaggtagcg
    53161 gaaaaacagt ggttgccgcg attgcgttat acgctaccgt tacagcaggt ttccaaggtg
    53221 cgttgatggt gcctacagaa atcttggcac aacaacatat ggaaagcttg caacagctgt
    53281 ttgatcccaa tgaagtaacc atcgcattga ttacggggtc aacgaaagca aaagaacgac
    53341 gtgagctcct tgaaaaacta gagcaagggg aaattaacgt cgtgatcggc acacatgcat
    53401 tgatccaaga tggagtcgaa tttcagaatt taggtctagt tattacggat gagcagcacc
    53461 gttttggcgt gaaccagcgg aaagtacttc gtgaaaaagg ctggcgacca gatgtcttat
    53521 ttatgacagc gactccgatt cctagaacat tagctattac agcatatggt gagatggatg
    53581 tatctatcat tgatgaactt ccagctggac gtatcccgat cgaaacacgc tgggtaagaa
    53641 cacctcaatt ggacagtgta ctggattgga cgaaaaaaga actggcaaga ggacatcaaa
    53701 tgtatgtcat ctgtccgctg attgaagaat cagaagcatt agatgtaaaa aatgcagtgg
    53761 aaatctatga aaaacttcgt gaactatttg caccaaaata tgaagttggc ttgttacatg
    53821 gtaaaatgaa gaatcaggaa aaagaagtaa tcatggaagc cttcaaagac aaccagatgc
    53881 agatattagt ttcgactact gtaattgaag taggggtaaa cgtgccgaac gcgaccgtca
    53941 tgctgatcat ggatgcggat cgatttggat tggcacagct gcaccaactt cgtggacgtg
    54001 ttggacgcgg aagtgatgct tcctactgta tcttagttgc taatcctaaa aatgaactag
    54061 gtgtggaacg gatgaaaatc atgacagaga ccaccaatgg ttttgtacta agcgaaaaag
    54121 atctggaatt acgtggtcct ggtgaagtat ttggcttccg tcaatctggt ctgcctcaat
    54181 ttgcgattgc tgacttggtg acggatggaa atgttttgga ggtagccaga gaagaggctg
    54241 cagccctttg gcaaataaag gattggcaga tgcttcctga gtatcagcct ttagcgcaaa
    54301 gcctccaaat agaaaatcag gataatcgtt atttcgatta gcagactgtg ctatacttta
    54361 tactgatgac ggaaaaagga gtggaaacaa tgaaaattgc cgtagatgca atgggcggag
    54421 atcacgcacc tcaagcaatt gtagaaggcg tggctttagc gcagcaagat tttccagaag
    54481 tagaattctt actttacgga aaagaagcag aaatcaagaa atacttaaca aatgaaaaaa
    54541 acatcacgat tatccataca gacgaaaaaa tcaatagtga tgatgagcca gtaaaagcaa
    54601 ttcgacgaaa aaagactgca tcaatggtcc ttgctgcaca agcagtgaaa aacggtgaag
    54661 cagatgctgt attttcagca gggaatacag gcgcgctact agcagcaggg ttgttcatcg
    54721 taggacgtat caaaaatatc gaacgccctg gcttgatgtc taccttgcca gtagtaggga
    54781 aagaaggtgc cggtttcgat atgctggatc ttggcgctaa cgcagaaaac aaaccagaac
    54841 atttgttgca atacggtatt ttaggttctt tttatgcacg taaagtccga ggtatcggac
    54901 gtcctcgtgt cgcactctta aacaacggaa cagaagcaac aaaaggtagt gaagtgacga
    54961 aaaaagccta tgaactcctt cagaatgaaa caagcttgaa ttttattgga aacgtagaag
    55021 ctcgtgagct actcaatggt gtggctgatg tagtggtcac agatggattt acaggcaacg
    55081 ctgttttgaa atctattgaa ggaacagcaa tgaatatgat gaatctatta aaatctgcta
    55141 ttctaaatga agggatcaaa ggaaaaatgg gcgctatgct tttaaaagac ggcttacgtt
    55201 ctttgaaagc agaaatggat tattccaaac atggcggtgc cgttttgttt ggcttgaaag
    55261 caccagtcat taaaactcat ggagcaacag gccccgatgc tgttcgctat acgatccgcc
    55321 aaatccacac aatgcttgag acagatgtag ttggacaatt agtcgaacaa tttgaaaaaa
    55381 gcgaagaata gtatttggat atctgaagcg atccgttcca ttgaaaaaaa ggtggacatt
    55441 cctgaaaaat gccagtaaaa ttagccatgg tgtaggtaat tgacaagtgt atgattcttg
    55501 gaggtgaaaa agttgactcg cgaagaagta tttaacaaag tagcgaaaat tatttcaaat
    55561 cattttgaga tagataccga taaagtgacg gatgaattga acatcaaaga tgatttaaaa
    55621 gctgattcaa ttagtattat ggaattcgtt ttagagcttg aagatgaatt cggtacggaa
    55681 atttcagatg aagacgctga acaaattgaa acagtcggtg gcgcagtaga ttatatcagc
    55741 agccatctta aataatttca aataaaagca aagaaggata aaaagctgca tgcagctttt
    55801 tatccttctt tttttaatct ttttgacacg taaaagtcat ttttctgata attctggcga
    55861 taaacagaaa ttatgttgca aagtaaagcg gttttctata taattattaa taattcatta
    55921 cacaaagata agaataacct ttgttataaa acgatttatt ctgtgtaatg tcctttttta
    55981 ttaagaaaaa cttccattta ggaagcagag aggggtattc gattgtccga taaccaatta
    56041 ttagaagttc agaacctaca tacaggattc cgactcaaag atgactatta cgatgcagta
    56101 gatgacgttt catttacttt agacaaaaac gagattttag caatcgttgg ggaatctggg
    56161 tgcgggaaaa gtaccttggc tactacgatt atgggattac ttgatcctaa taacacaaaa
    56221 attacaggtg agattttata taacgatctg aatttaatcg acctgaacga aacgctctat
    56281 aataaaatcc gcggaaatga tatcggaatg attttccaag atccgttgtc tgcattaaat
    56341 cctctcatga gaatcgaaga ccagatcaaa gaaggacttt cctatcacac aaagatgaat
    56401 gcagaacagc gtcaagctcg tgcactagaa ttgctagaac aagtagggat tcctaatcct
    56461 gcgagagttg gacgccaata tccccatgaa ctatcaggcg gtatgagaca acgtgtcatc
    56521 atcgcaatcg caatcgcctg caaacctcca atcgtcattg cagatgaacc gacgacagcc
    56581 ctagatgtaa ccatccaagc acagattctt gatttactga aagaccttca ggaagaaaca
    56641 aaaacaggga tcatcttgat cactcatgac ttaggagtcg tagcagaaat ggctgatcga
    56701 gtggcagtga tgtatggcgg acagttcgtt gaagtagcag gtgtaaaaga actgtttgat
    56761 catcctaagc atccttatac acgctcttta ctgaactcta taccacaaga aggaaatcat
    56821 gatgcagaac tccatgtgat cgaaggtgtc gttccttctc tgaaaaacat gcctcgtaaa
    56881 ggttgtcggt ttgcaccacg tattccatgg ataccagcaa gcgatcatga agaagagcct
    56941 atccttcatg aagtcgcacc gaaccacttg gtacgttgct cctgctataa gcatttccac
    57001 tttagagacg aaaaaggaga ggtgtagatg acagaattaa tcacaatcaa agacctgaaa
    57061 gttcattacc ctattcgcag tggattcttc aatcgagtaa cggatcatgt atatgctgta
    57121 gatggtgtgg attttattat cgaaaaagga aagacatatg gtttagtggg agagtctggc
    57181 tctggaaaat ctactacggg aaaagccgtt gtgggattag aaaaggtaac agccggacaa
    57241 atcatgtatg aaggaaaaga tgtaacgaag cgaagcaacc gcaaaaaaat gggttataac
    57301 aaggatgtgc agatgatctt ccaagattca atgtccagtt tgaatcctaa aaagcgagtg
    57361 ttggatatca ttgctgaacc catccgaaat tttgaacggt taagcgatca agaagaaaag
    57421 aaaaaggtaa aaggactgct agatatcgtt gggatgccgg aagatgcgct atataaatat
    57481 cctcatgaat tttcaggcgg acaacgtcaa cgtctaggag tagcccgtgc tgttgctacc
    57541 aatccaaaac tgatcgtagc cgatgaacct gtatctgctc tggatctatc tgttcaagcg
    57601 caagtattga atttcatgaa acgaatccag caagaatacg gcctcagcta tttattcatt
    57661 tcacatgatt taggtgtagt aaaacacatg tgcgacaata tcgcaattat gtacaaaggt
    57721 cgatttgtag aaatcggtac aagagaagac atttataatg acccgcgaca tatctatacg
    57781 aaacgtctgc tttcagctat tccgcaaatc gatgtagaaa atagggaaag ccacaaagaa
    57841 aatcgacgaa gagtagaaca agagtatatc caaaaccaaa aagaatacta tgatgcgact
    57901 ggtcgagtct acgatttgcg tacgatcaca actacacaca aagtggcgtt gaaagatgga
    57961 ggtgcaagct aatgtggaaa acgattctgc gacgtgtttt attgatgatc ccgcaagtta
    58021 ttattttaag tgttctgatc ttcttattag cccaagcgat gccgggggat ccatttaccg
    58081 gtttgatcaa tccaaatcag gatccggcag tgatcgaaca gatgcgacaa gcagctggct
    58141 tgaatgaccc atggtatgtc cagtacttcc gttggatcgg aaatgctttg caaggcgatt
    58201 ttggtcaaag tttcttatac aaacgctctg ttgctgactt gatcggcgaa cggatcatca
    58261 acactttata tctatcgatc ttgacagttg tccttactta tttgattgct gtgcctctgg
    58321 ggatgcttgc tggccggtat caaaattcaa tcttggataa gattgtcgta atctacaact
    58381 tctttagttt tgctgttcct ttatttatct tcggcttgat catgctattt gtttttggtt
    58441 atcgtttagg ctggttccca acaagtggat cacaaacact aggtttgcaa ggtggattta
    58501 tcactcaatg gatagaccgt ttgaaatata ttctgttacc atcaattacc caagcatttt
    58561 tggcgactgc cgtcacaatc caatatctgc gaagtgaagt aatcgattcg aaatcgctcg
    58621 attttgtcag aacagcccgt tccaaagggg taccgacgaa taaagtattc agtcgacata
    58681 ttttccgaaa tgcagcgttg ccgatggctt ctcaaatggg gtatgaaatc acttcgttga
    58741 ttgctggttc tgtagttatc gaaaagattt ttggttatcc gggagtcgga aaactattta
    58801 ttgattccat cggccaacgg gactataccg tgatcacatc cttagtattg attttaggaa
    58861 tcgcgacgct agtggggaca ttactttcag atatcatcat gagtatcgtt gacccgcgta
    58921 ttcggattca ataaaaccaa tcagaaagga gaatggatcg atgagtgaaa aaaacaatac
    58981 agcagttcaa gaaagtattc ctcctatggg gatacgaatg attgctcgag aatttaaaaa
    59041 agataaaatt gctatttttt cattaggttt gctagtggtt ttattattag tgatctttat
    59101 cggtgcgatg ctgaccgatc aagacaaagt gatgtatgtt agtatcttcg acaaatacgc
    59161 tgcaccagga acagtttcga cacaaggaca aaagttcatt ctgggagcag atgaaggcgg
    59221 acgtgatgta ttaggacaat tgatcatagg tgctagaaac tctgttacga tcggatttgc
    59281 gatcacgatc cttacttcga tcatcggggt tggattaggg atcatctctg gctactacgg
    59341 agggatcatt gacaacatct tgatgcggat cgttgatttt atcatgattc taccgatcat
    59401 gctgatcatt atcgtcttcg tatcgattat ttccagatac aatgtctggt cctttatatt
    59461 catcatgagt gcattttatt gggtagctaa agcccgatta ttccgaagca agacattatc
    59521 tgaagcaaga agagattatg tcagtgcctc aaaaacaatg ggaaccaatg attttaaaat
    59581 catgttccgc gaaatcatgc cgaatctaag ttcattaatc attactaatt tgacgatgaa
    59641 ctttgcggca aacatcggta tcgagacaac tttgactttc ttaggtttcg gcttgccggc
    59701 aaatgtacct agtttaggta cattgatcgg ttatgcaagt aacggagaag tcttagtgaa
    59761 tcggcaatgg atctggttac ccgcatcaat ccttatttta gttttgatgt tgagtatcaa
    59821 ttatgttgga caagcattca agcgttctgc agatgcgcgc caacggttag gctagtatag
    59881 aagcgaaggg ggaaatagga atgaagaaaa cgatttttgg atttgcagcg ctgatgtctg
    59941 ctgtcgttct tgcagcatgc gggggcggag gtacagatac ctctggaaac aactctggag
    60001 gcaacggcaa taacaaagag gaattaactt ttccaattgc aacaaaaaat aatgatgatg
    60061 caatcgaaga cggcgaatta gaagctgcgg tcgcaacgga cagtcagttt aaaggattgt
    60121 tccagtggga gttttaccaa gattcatatg atgcagcttt tatggctcct tctcatgaac
    60181 cgctatttgc aaacgatgac gatttcagaa tcacagacga cggagctgca acgcttgatc
    60241 tagatgaaga tacgaaaaaa gcgacaatca aagtaaaaga caacgtcaaa tggtcagatg
    60301 gtgaagatgt aacggcggac gatgtgattt tcccttacga aatcattggg aataaagatt
    60361 acactggtat ccgttatgac gacactttcc gtaacatcgt aggtatggaa gaatatcatg
    60421 atggaagtgc cgacacaatc tccggaatca aaaaaataga cgacaaaaca gttgaaatcg
    60481 aatataaaga agtaaaccca agcatgcttc aatcgggtgg tggtatctgg tcatatgcag
    60541 caccaaaaca tacgttagaa ggtgtagcaa tcaaagatat ggaatcaagc gatcaagtac
    60601 gtaaaaatcc agtgactttt ggcccttatt acatgagtaa tatcgttgca ggagaatcag
    60661 tagaatactt gccaaacgag cactactgga atggtacgcc taaattgaaa aagatcacga
    60721 tgaaatctgt accgatggca agtgcaactg aagcattgaa ctctaaatta tatgatatga
    60781 ttttccaaat gccaacagat acttacgata cctataaaga aattgctggc tatacaaact
    60841 taggccgtga acaaacttct tatacttatc taggattcaa gttaggtaaa tggaatgcag
    60901 atacaaagag tgtggaatac aatccagatt caaaaatggc tgataaatca ttacgtcaag
    60961 caatgggcta tgcattagat aataatgcag taggtgaacg tttctatgca ggtttgagaa
    61021 gcaatgcgac ttcattgatt cctccagtat tccagtcata ccatgatgat gagatgaaag
    61081 gattcacaca agatctagac aaagcgaaaa aactattaga tgatgctggc tacaaagatg
    61141 tggatgacga tggtttaaga gaagacaaag atggaaataa actaacaatc aactttgctt
    61201 cgatgtccgg tggggaaaca gcgcaaccat tggcagatta ctatatccaa caatggaaag
    61261 aaattggttt ggatgtccaa tacacaacag gtcgtctgat cgaattcaat tctttctatg
    61321 ataaattaga aaacgatgat cctgaaattg atgtttacca agctgcttgg aatacaggaa
    61381 ctgatccaaa tccaagtgga ctatggggac caaatgctgc gttcaactac actcgttatg
    61441 aatcagaaga aaatacaaaa ttgatcaacg atatcagttc aagcaaagca tttgatgaag
    61501 agtaccaaaa agaagcatat caaaaatggc aacaatatgc atttgaagaa gcatttgcga
    61561 ttccaacgtt gttccgtaat gaagtattgc cagtcaacga ccgtgtcaaa gactgggatt
    61621 ggtcttatga tgtaccaaac ccatgggcaa atgtttctgt tacagcagat tcacgttcat
    61681 aatcactaaa aatgaccgaa aaagggcgat gtcctttttc ggttttttga tatttttctg
    61741 tttatttctc taatattcag gtcaaggcgc tgatttcatg acttctccaa taaacggtgt
    61801 tcaaagtcca gtttctcggt attaagtcaa atttatgcaa aaatatggaa agccattttc
    61861 acaaaatttt ttcttcatac tcgaaactaa acgaattttt cacaacttct cgtcaatcac
    61921 taggattatt aaagctttac ttaccttttg accgaaccat ttcaaatttg acaaaaaacc
    61981 agtataataa ggaagtatcg aaaaaatatc cataaaagga atgtggaaaa tggacaacca
    62041 gctgacaaaa gaactaaaag aaaactataa catcgttttt cacgatctaa atttgcttga
    62101 acaagctttt acccattcat cctatgtgaa tgagcatcgc aatctgcaat tatcggataa
    62161 tgagcgactt gaatttttag gagatgccgt tttagaactg atggtttctc agtatttatt
    62221 ccgtttatat cctcatttgc cagaaggaaa attgacaaaa acacgagctg cgatcgttcg
    62281 cgaagacagc ttgtcgaaat ttgccaaaga atgtcacttt gatcaatatg ttttgttggg
    62341 caagggagaa gagaattcag gaggacgtac gcgaccagca ttgctttgcg acctattcga
    62401 agctttttta ggcgcgttat atcttgacca gggattcgac acgacccatg cattcatcga
    62461 aaaagtgatt tttcctaaag tcaaagcagg tgctttttca catgagatgg atcataaaac
    62521 aaaactacaa gaagtcttgc aaaaatcagg tgatgtatca atcgaatacc gattgatcaa
    62581 tgaagaaggt ccagctcatg aacgagtatt ttggatcgaa gtttatgtag atgatcaatt
    62641 gattggcact ggacaaggga aatcgaaaaa attagcagaa caagctgcgg cggaaaatgc
    62701 attggcagca ctgtaaaagt aagtgaggga aagctttgta tttgaaacga attgaaatag
    62761 ccggttttaa atcatttgca gacaagacag tcattgattt tgaaaatagc gtcacagcag
    62821 tggttggacc aaatggcagc ggaaaaagca atattacaga ggctatccgc tgggtattag
    62881 gagaacaatc tgctaaaagt ctgcgaggcg gaaaaatgcc ggatatcatc tttgccggtt
    62941 ccgatacccg taagcagctg aatattgcag aagtcacagt gattttagac aatagtgatc
    63001 attatctgcc attggagtac aatgagatca gtgtaacccg ccgttatcga cggactgggg
    63061 aaagcgagtt tttcatcaat aaacaaagct gcagattaaa agatatccaa gaattgttct
    63121 tagattctgg attaggaaaa gaatcgttct caatcatttc tcaaggaaaa gtagaagcga
    63181 ttttcagtag taaaccggaa gaccgtcgtg ggatctttga agaagcagct ggtgtcttga
    63241 aatacaagca gcggaaaaag aaagcagaac agaagctttt tgaaacagaa gacaacctca
    63301 gccgcgtcca agacatcatc cacgaattgg aagaacagtt aactcctttg gccgcccaga
    63361 gtgaggcagc aaaggagttt ttacggctaa aagaaacact gactcaaacc gatgtctctt
    63421 tgatggttgc tgaaatcaag acagcaaaaa aagattggga caacaaacaa gcacagcttg
    63481 cgaaattcaa tctagaatta ggcaaactat ctgagtcgat ccaagaacaa gaatccattt
    63541 tggcgaagca gcgtaaagaa aatgcgcaag ccgatcgtct gatcgaaaaa aatcagcaag
    63601 tattattaga tctttcggaa aaactgaagc agacagaagg acaaaaagat gttttgcagg
    63661 agcggacaaa gcatacacaa aaaagctcgc aagaatatca aacttcatta gcagaagcac
    63721 aaaaaaaggt taaacatttt gaaaagcttc aagaaagctt gatgaaagca gcagcagaaa
    63781 aagaaacaga gatccaaaaa gctgaagcaa atctcataaa aacacagcaa gaattagaaa
    63841 aataccaaaa atcaacgaaa gaattattgg cagaattaag agatcaatat gttgatttga
    63901 tgcaagaaca agcagctgta ggaaacgaac tgaagtatct agaacgtcaa tatatacaag
    63961 aaacagcaaa aagcaagcag acgttagcca agcaatcgga agtagaagca tcagtagacc
    64021 gattgatctt acaaaaagaa gaattgacac aaaagcaggc gcagctaaaa tcatctttaa
    64081 ctgaaacaaa agagaagtta gaaatgatcc aacaaaacgg gaaaaagttc caagaagcat
    64141 tagcaaaaga acagcctaaa atgtatcaat taatgaatca agtccagcag ctacgtgctc
    64201 gtaagaaaag ccttcaagaa atccaagaaa actattttgg tttttaccaa ggggttcgat
    64261 tggtcttgca gcataaacag cagctttcgg gtattgtcgg agcagtggct gaactgatcg
    64321 atgttcctgc agatttcacg ttagctatcg aaacagcttt aggtggggca gcccagcatg
    64381 tcatcgttga aaatgaaaaa gatgctcggc aagccatcac gtatttgaaa cagcaaagag
    64441 gaggaagagc aacattcctt ccactgacga caatcaaacc aagacaatta ccggctcata
    64501 tattgacaca agcagcagct gtagaagggt ttatagggat tgccagtgaa caagtcagtt
    64561 atcccgatca aatccaaact gtggttcaaa acctgctagg aacgatcctt ttagccaaag
    64621 acttaactag tgccaatgca atcgcgcaaa cgatccgcta tcaatatcga gtggtttcct
    64681 tagaaggtga tgtgatgaat gccggaggat ccatgacagg tggagcgaat aaacgaggaa
    64741 accaaggcag tttgtttgta caaaatcaag aattgaaaca gttgactagt gaatttgaag
    64801 aagcagataa acaactccaa gcacaagaaa aaaaagttca ggagctgcaa caagaaacag
    64861 ctcgtttagc cgaagaacaa gaagttttgc gtacacgagg cgaacaatta cgttttgaag
    64921 aacaagaagc gacaaatcag ctacaaaaca tcataaatga acttgaacgt tttgaaaaag
    64981 aaaagcaaat ctcaactttt gagacacgtg aactgcagca gtttatcgaa gattacgaaa
    65041 agcaaacgaa cgaattgaaa gacaaacaaa cagatcttga atcgcaacga caaaaaatcg
    65101 acgaagaaat caaatcgtta agtcaagaaa gcgaccagat ggaagccaga agagcacaag
    65161 tacaaagtca aaaagcacaa gaacaagcag atcttgctgt cttgaaagag cagtttaatc
    65221 atctgcagat ccagctccgc ggtgcccgag tccaaaaagc agaggcaacc gaacgtcaag
    65281 aagcgataga aaagcagttg gcgacattga cagcagactt ttctgatcat gaagtcacag
    65341 aagaaagttt ggaaaaacaa attaacgaat tatctgctca gcgagaaaca ttaaaagccg
    65401 agttggcaaa agccaaagag cagcgagata gaaggcaaaa ggagatcgat aagcttgaag
    65461 cagtcttggc agagcgtaat cgtgagcaaa aagcacgact gtcggaacaa tccaaattag
    65521 aagtccaaaa agaccgagct gaaatgattt tagataatca tttgacctat ctgcagtcag
    65581 aatatcagct gacatttgag aaagcttccc aagactatca agaaacaacc gatatcgaag
    65641 acagtagaac aaaggtttca tccttaaaag aacagattga aaaactggga ccagtaaact
    65701 taaattcaat cgaacaatac gaacaagtca gtgaaagaca tacattctta gcaacacagc
    65761 gagatgatct gttagcagcg aaaaaccaat tgtttgaaac gatggatgag atggatgatg
    65821 aagtgcggac aagattcaaa gaagtatttg aagctatccg tcaagaattc aaaattgtct
    65881 ttccaaatat gtttggcggc ggtaaagctg aattagtact gacggaacca actgatttgc
    65941 tgaaaacagg tattgagatt gaagtacaac ctcctggcaa aaaactgcaa agtctaagtt
    66001 tgctttctgg aggagaacga gcacttacag ccatcgcgtt gctcttttcc attatccgtg
    66061 tctgtcctgt tccgttttgt atcttagatg aggtagaagc agcgttagat gaagccaacg
    66121 tgaaacgttt tggtcgttac ttgagcgatt tccaagatga cacacaattt atcgtagtca
    66181 cacaccgcaa aggaacgatg gtcgcagcag atgtactgta cggagtgacg atgcaggaat
    66241 ccggtgtatc aaaaatcgtt tctgtacgga tggaagatat caatgaagag gcgaaaatca
    66301 acgaaacgaa agtgtaggaa cgaaaatgat caaattagta gcaatcgatt tggacggtac
    66361 attattaaat gaacaaaagc tgatttcaga tgagaataaa caagcattag cacaagcaaa
    66421 acagcaaggg gtcaagatcg ttctatgtac aggacgcccc ttagctgcga tggcgcatta
    66481 tctgcaagaa ttaggactgg tagatgaagg tgactacagc atcactttca acggcggttt
    66541 ggtacaaaaa aatgatactg gtgaaatcat ggaaaagaaa gtgatggaag tatcagatat
    66601 ccaacgctta tacaaactgg ctcagcaatt ggatctgcca ctagatgttt tgtctgacaa
    66661 tgtggtttta cagctgccta gtgccccaag gaagcaatcc ttgtacaatg tgttgaataa
    66721 gctgctgcaa ttccaaccag cggatataag cgatattaca gatgaatttg tgcttaataa
    66781 ggcagtaatc gcttatgacc aagaagaatt agatcctaaa atcaaagaaa taccagcaga
    66841 attccatgaa atgtatgaga tcatcaaaac aagaagtatg cttttggaat ttatgccaaa
    66901 aggtgttaca aaagcttatg gtatttcttt attggcaaaa gatcttggtt tgaaacaaga
    66961 agaaatcatg gcgatcggtg atgaggaaaa tgatttaccg atgatccaat atgctggact
    67021 aggcgtagcg atggagaatg ctgtgccttt cgtcaaagaa gcagcagatt tcgtgacagc
    67081 cagcaatgaa gaaaatggcg tagccaaagt gatccaaaaa tttgtattag agtaggggga
    67141 gtcaaatggg attttttgat aaaatcaagc gtgcttttac tggcgaagag aaaacagaag
    67201 aagttgaagt acaggagaag tacgacaagg gtttagaaaa gacgcgtaaa acatttggtg
    67261 aacggatgaa tgagttgttt gccaattttc gaacagtcga tgaagacttt tttgaagaat
    67321 tagaagaaac acttatcggt gcagatgtag gttttgaaac agcgatacga attaccgaag
    67381 cattacgtca agaagtgaaa ctcagaaatg cgaaaaaatc ggcagaagtc caaaatgcga
    67441 tcatcgagaa acttgtggat ctctatgaag cggaaggaat caatgaagta aatgaactaa
    67501 atatccaaga agacggacta acagttattt tgtttgtcgg tgttaatggt gtagggaaaa
    67561 caacaagtat tggtaaactt gcccatcagt tcaaaaacga aggtaaaaaa gtattgatgg
    67621 cagcagcaga taccttccgc gctggagcaa tcgaccagtt agttgtctgg ggcgaacgcg
    67681 ctggtgttga agttgttcgt ggcaatgcag gaggcgatcc agcagctgtc gtattcgatg
    67741 ctttggaaag agcaaagaca gagcaggcag atgttttact agttgatact gcaggccgac
    67801 tgcaaaataa agtgaacttg atgaacgaat tagaaaaaat aaaacgagta atccagcggg
    67861 aattaccaga agcaccccaa gaaacacttc ttgtattaga tgcaaccact ggtcaaaacg
    67921 cgatggttca agcaaaacaa ttcaaagaaa caacagatgt gaccggtctt gttttgacaa
    67981 aattagatgg tacagcaaag ggcggtatcg tacttgctat ccgtaatgaa ttgcatttac
    68041 cagtgaaatt ggtcggtctt ggtgaaggaa tcgatgactt ggagccgttt gatccaaatg
    68101 attttgtcgt tggattgttc aaaggattac taaaagaaga ataacccatg tctaaaaagc
    68161 aggagctgtg acaaaagttg cagctccttg tttgaatgat tgtttttaac atagctgtgt
    68221 ttttgatgcc ttcattttta tacttatttg taagcggttc tcaaacaatt tcgttctttt
    68281 tcttagactt tttgattata attagattca tatgaattgg aggtcactta atgatgaatt
    68341 ttaagcaatt attattgcat gtcaatgcgc gcccttttat cgaccaagct cgatttggta
    68401 tagaacggga aggacagcgg gttgatcttg caggaaatct agcaaaaacc gatcatccag
    68461 caatctttgg cgatcgatcc tatcacccct atatccaaac agattttagc gaaacacaaa
    68521 cagagatgat cacccctgtt accgattcta ttcccgaatt atttcagtat ctcgctgctg
    68581 tttatgatgt gactgctcgt tctataccga aagaagagat gatctggcca ttaagtatgc
    68641 cacctgcctt accggaaaaa gacgaagaga tcattattgc aaaattaaaa aatttcgaag
    68701 atgtcttgta tcgacgttat ttagcaaaag aatacgggaa gcgaaaacag atggtaagcg
    68761 gtattcattt caattttgaa tttggcgatg agttgctaag aacattgttc agccatcagg
    68821 aagaatttca agatttttcc gaattcaaaa cagaacttta tttgaaaaca gcgagaaact
    68881 ttatgcgcta tcgctggatg atcacttatt tattcggtgc ttcaccaatg agtgagaaga
    68941 actacttttt agatgaatca catccgcaag aacctgttcg cagtattcgc aacagtgcgc
    69001 tgggctatac gaatcatcca aatgtgaaag tttcctatgc ttctatgaaa cagtatttag
    69061 cagatatcga gcgaatgatc gaagaaggga aactttcgga ggagaaagaa ttttatacac
    69121 cgcttcgttt ccgaggcgga aagaaagtcg cagatttagc aacaacaggt gttcgttata
    69181 ttgaattgcg gaatatcgat ttgaatcctt atgcaagatt gggtatcaat ccagagcaag
    69241 ttcggttctt acaattattc ctgatgtaca tgttatggac agaagaaaaa gaagactgtg
    69301 accaatgggt agcagaaggt acgactcgaa ataacaaagt agcactagaa caaccatcag
    69361 atcaaacaga atttcatcaa gaaggtagag aaattcttga aggaatgaag caaatgctgg
    69421 ttgaattgga ttggttggat tctctttatt tagtagagga agcattgact caaatggatc
    69481 atcctgaaca aacgttagca gcgaaactct atcaagaagc acagctatcg agtcagcaag
    69541 aagttgctgt ggcattggga catcaatatt ataaagaaag ccatgaacgt ccctatcagt
    69601 tggccggttt tcgcgaaatg gaactttcta ctcagatatt tatgttcgat gccatccaaa
    69661 aaggtgtcca agtcaaagta ttagatgagt cggatcaatt tttgcgtctg caattccaag
    69721 accatgtaga atatgtgaaa aatgcaaaca tgacaagtaa ggacagctat atcgtcccac
    69781 ttatcatgga aaacaaaaca gtaacgaaaa aagtcttaaa agaagctggg ttccgggtac
    69841 caggaggcgc agaattttca tccatggaag aagcggtaaa agcttatccg agatttgccg
    69901 agcaggcatt tgtgatcaaa cctaaatcaa ccaattacgg gttaggtatc acgattttca
    69961 aggaaggagc tagtctggaa gattatcaag caggtttagc cattgctttt cgtgaagaca
    70021 gttcggtttt agtagaagaa tttatgccgg gaacggaata tcgtttcttt gtgatcgacg
    70081 gagaagtcca agccatcatg ttgcgagtcc ctgctaatgt tattggtgac agtatccgaa
    70141 cagtgaaaga acttgttgaa gaaaaaaata gtgacccttt gcgtggaacg aatcatcgtg
    70201 cacctttaga attgatccaa ttaggtgaat tagaacaatt gatgttgaaa gaacaagggt
    70261 tgacgatcga atcagttccc caagccaatc aaattgttta tctaagggag aactcaaata
    70321 tcagtacagg tggtgattcg atcgatatga ctgatgaatt ttctgaagct tacaaaaaaa
    70381 tcgccgtttc tgcggtagaa gcactaggag ctaagattag tggtatcgat ttgattatac
    70441 cagataaaga aatcgatcct acaactgata aaaaagcata tgggatcatc gaagcaaatt
    70501 tcaatccagc catgcatatg catgtttatc cttttgcagg aaaaggcaga agattgacga
    70561 tgaatgtatt gaaattgtta tatcctgaag tattttaaaa gaaaaagctt ttgacagatt
    70621 gtcctatttg atgtcaatct gatcaaaagc tttttttgca tgtaaaaatt tatgtgtttt
    70681 ttcgttttaa aataaacgat cccagaagct ttttttcttt tttggttttt ctttttcagc
    70741 tggcgaagag ttttcttctg ggtatttttt ttcgatatca atcacaggtt catttactgc
    70801 ttcttttgct gttaagagca atccgaattc ctcaggattt gcagtgacgt aatcgttgac
    70861 tacagtgaaa cggctattgt ttttcgttgc tagctggata tattggtttt ggacagaaag
    70921 gggtagagcc ccattcaaga gtatggaagc atctggatgt tttttgagtt cttttaaaaa
    70981 gttttctttg ttttttggtt ggatcatttc agcaacagtc atactaagat agcagcgttc
    71041 cctgaatgtc cctaaatatt tatgctgttc ttcagggttc actaaaggtg taccataaag
    71101 tcccttatcc aaatggcttt ttacatcatc ttgtgccata aaaaataccc tccttcttct
    71161 ctttgtcttt cttacgatta tagcatattc ccaataatac ctaaaacata tgaaaaagcg
    71221 gttgcttttg ttcaacgatt cacttaaaat aagaatgaaa atcattctca attagaaagg
    71281 tggattggat gcgctacgga cgactagtag cattattagt tattttaagt agtatttcat
    71341 tgttcatcgg agtatcgaat gtacaattgt cagaactgct ccagttagat gggcaaagct
    71401 tacaaatctt gctagtgact cgtttgccac gaacgatttg tttgatcctt gtgggagcga
    71461 ccagtagtat ttgtgggttg atcatgcaac atttgaccca aaataaattt gtttcaccaa
    71521 cgactgcggg cacgatggat agtgcgcgtt taggtatttt agtggctatg attttcctac
    71581 ctggtgcatc cttgattgtc cgatccttga cagcattttg ctttgctttt gcaggaacat
    71641 tgcttttttt atcccttacg cgattgttcc tccagaaaaa tcaattgatg cttccgttga
    71701 ttggagtcat gttagggaat atcattggtt cagttgctac tttttttgct tatcaattcc
    71761 agttggtaca aaatatgact tcatggcttc aagggaattt tgcgactgtg atacgaggaa
    71821 attatgagtt attgtatgct gttgttccat tgttgctctt ggtttggcta tttgcttacc
    71881 gatttacgat cattggtctg ggcgaatcgt ttgcagtaaa tctgggaata gattaccaga
    71941 agatgcagtt tttaggcatt ggcttagtat cattggctag tgcagtcatg ttgatcatgg
    72001 taggaagcat tccttttatc ggagtgatca ttccgaatct tgtctctaga atatatggag
    72061 atcaagtgca caaaacaatt ggcgtaactg ctttagcagg tagtttgttc ttagtatttt
    72121 gcgatcttgt cgctcgcgtg ttgatttttc cttatgaaat accagtcagt gtgatcgttg
    72181 gaatcatcgg tggggctatc ttcctttatc ttctgataag gggacgacga tatgcttaaa
    72241 aaaaagccat ctatttttat tggtatgctt gttcttgttt tatttgttac aggaatgtac
    72301 ttgacgatcc atacatatgg aaactggcat tttgcatgtg cattgaggag taagaaaatt
    72361 tttgcattta tccttgtctc gctagctgtc agcagtgcca caatcagctt tcagacaatt
    72421 acgcataacc agtttttgac tccttcagta ttgggacttg agtcgttata tgtccttgtc
    72481 caaacggtcc ttttcttctt tatcggagga gtgacgatgc ttcagcagac aggtacagtt
    72541 tttttcttat tgaacgttgg gctgatggtc gggctgagtc tattgttatt tttctttctt
    72601 ttgaaaaaaa tgagagcgaa tcttttttta ttactgatgt cagggatgat tttaggtacg
    72661 ctttttggta gtgtcagtac ctttttacaa gtcatcatgg atccgaatga atatgatttg
    72721 cttcaaggca gattatttgc aagctttggc aatatccaga gtcagtatct tttgcctgct
    72781 tctttgatca ttatcggctg ctggtacctg ttatttcgat atcgacatgt gctagacgtt
    72841 ttccatctcg gaaatgaaca agcagtaaac ctcgggatag acacagatcg ttttgaattt
    72901 ttgattttat gttgtgtcag tcttttgaca gggacatcaa cggctcttgt gggtccagtt
    72961 acttttttag ggtttattgt cgcaaatatc agttatcgtg tcttttctac ttatcgtcat
    73021 ggtcagctat ttatcggaag ctttctggta gctatgctgt ttctgacagg aggacagctc
    73081 gcagtagagc agattttcgg ctggaataca acagtgagtg tcgtcgttga atttgtcgga
    73141 ggaatttatt ttgtcacaaa attgattcgc gaaaggaaga gtatcctatg attgatttac
    73201 aaaaggtcaa taaactgtac catggaaaat cagtggttaa agatgtacag ttaacgattc
    73261 ctgaaaagaa attgactgct tttatcggtc caaatggtgc tggaaagtca acggtgttgt
    73321 cgatgatcag tcgtttgata ccaaaagata caggtgaaat ctatttagac cataacgaag
    73381 taaaggcttg gaaatccaat gaattagcca aaaaattatc gatcctacga caatcgaatg
    73441 aaataaatgt ccaaatcacc gtgaaagaat tagtagaatt tggacgattt ccttatacga
    73501 aaggaaaatt gacgacaaaa gacgaggaaa tcgttcagga atcactggaa caccttgggt
    73561 tagaagaaat agcggatcaa tcgattgaca ctctttcagg cggtcaactc caacgtgcgt
    73621 atattgcaat ggtgctagcg caagacacgg attatatttt actggatgaa cctttgaaca
    73681 atctagatat gaattttgcg gttcaaatca tgcagatttt aaaaaattta gtacaagaaa
    73741 tcgggaaaac aattgttatt gttcttcatg atatcaactt tgctgcaagt tatgcagacg
    73801 aaattgttgc aatgaaagat ggacagttat ttacgcaagg aacgacccaa gaaatgataa
    73861 aaaaagatgt attggatgaa ttatacgaaa tggacatacg gatttgtgag attgaaggcc
    73921 gcagattctg tctttatttc taaaactaac attaggagag aagagatgaa aaaattagta
    73981 gtaggtatct tgattctagc atcatttggt ttagcagcat gtggaaatag caacaacacc
    74041 agtcaggcag acacaaaaaa aagcagcaca caaactgaaa ctacattgac gataacagat
    74101 agtaatggag accaaatcga ggtgccaaat aatccagaaa aagtcgtcgt attcgacaat
    74161 ggttctttag atacgatgga tgctcttggt gttggagata aggtagttgg agcagctaca
    74221 agcagccttc ctgagtatct ttcatcctat aaaaaagtag aatcagcagg tggaataaaa
    74281 gaaccagact tagaaaaaat taatcagctt cagccagatc taatcatcat ttctggccga
    74341 caaagagatt ttcaaaaaga tctaagtcaa attgctccga ccattttcct ttctttagat
    74401 gcgaaaaatc catgggaatc tttccagcaa aatgtcactg ctttagggga gatctttggt
    74461 aagcaagaag aagctaaaac tcaattagag gaattatcta gtgcaatcga tcagacgaaa
    74521 aagaaagcag aagctactga caaaaaagca ctagtcacac ttgtgaatga agggcaatta
    74581 tcagcctacg gatctggttc tagattcggt ttcatccatg atttatttgg ctttgaacaa
    74641 gcagatgatc aaatcgaagc atccactcac ggtcaaagtg tttcttatga atatgtgctg
    74701 gaaaaaaatc cggatatctt atttgtagta gacagaacaa aagctattgg tggagacgac
    74761 agcaaagatg atatttctgc caatgaactg gtagcacaaa cgaatgctgg taaaaatcag
    74821 cagatcatct cccttgaacc agatgtctgg tatttaagcg ggggcggttt agaatcaatg
    74881 aaactgatga tcgaagatgt gaatcaagca tttaaatagc ataaataaca gaggattaaa
    74941 ctctcatact atgtaacaat aatgtttctt acattcttaa atagcaagag cctatcctca
    75001 aagaagttat caaaaaccat ccgtaaaatc gtattgatga ttttacggat ggtttttatt
    75061 tatttgagaa atcttttttc aacgtgaatt cattatctac atcatacggg ttgattctag
    75121 aatggggagt ggcaaaaaat agtgtgatcc attttttcag catcaagatc gcctcctttg
    75181 atttgaatca aaggtacaaa gcgaaactta gataaaactt aaatttagat atttttattt
    75241 tgtaccgatc tcttctttaa acgatgttcc atctgctgct tctcggcaat aagtccacta
    75301 gactagaaaa tatgagaagc tattttctgt ccagcgttct tattactcaa agcaaatcgc
    75361 agttgtcaca atctcttcta aacgatgttc catctgctgc ttctcggcaa taagcccact
    75421 agactagaaa atatgagaag ctattttctg tccagcgttc ttattactca aagcaaatcg
    75481 cagttgtcac aatctcttct aaacaatgtt caatcagctg accttcggta ttagctcaaa
    75541 gcactgattt cacaacctct atttaggcat gtgtctcagc aagcggttga tagttgatga
    75601 tctgcatttt tcgataaaaa tcggcgggaa gcttactcca ccgttttaat aattgataga
    75661 aataactctc ttctgctttg attttatact cgcttaatat caatccaaaa ctattcgttt
    75721 tttttgaagc taatttttta taaatatcca atgctttggt tttatcttca ttgacaaaga
    75781 gtgtttttac ttcaatataa gaaccctcga tattttgtgt ttcttcgata aaatagatgt
    75841 tttttatata ttccttcata gtgattcctc caaagaagtc ttttatttga taagtttact
    75901 ctaccgaaga gttgttaata atccgtgaac aaaaagtaat tctttttcca atagttgtta
    75961 aaggtttttt attttttata gtattttttg ttatttaaga aaggaataag gttaaacaaa
    76021 taacaggttg ctttttgggt ctttttccgt tcaagaaact gaacgaagaa aagggaaagt
    76081 tgtctctttt gtgataagag aaatcataat aaacgaatga cttactttcc aataaattga
    76141 gaacagtcag aaccagacaa attagttgca tctgatgaat aaagtgatta ttctatgaca
    76201 tgaggtgata cagatgaaat tagaagtgac aaaagaagca caagaagtat tgaagaataa
    76261 attagaagca gacgatcaac ttttattaga tatcgaaaac ggtgacggtc catttgcaga
    76321 aagccaagtc agttgccaat tggatacggc atttcgattg atcattgtca aaaaagatac
    76381 acaaacagat ctaagtcttt attctgtagt ggctgataca ccagttggac caatcaaaat
    76441 caaagacagt gcaatacttt acatggatga tccatccaca cttgctttag aaccaactta
    76501 taatagttta caattaaaag gaccaagcgg cttgagaaaa ggcaacttgc aagttgttcg
    76561 aaaagactag agggataaaa tgggaaaatt ggcgatcatc agcgacctac atgtggacat
    76621 caataaattc ggcgaatcag agctgatgca gctttggcaa gttttgcaaa agcacaagat
    76681 cactcggctt catctagccg gagatacggc taataaagta gaccgttgcc tagctgttgt
    76741 cgaatttttc gtcaataaag gattatctac gacatttcat ttcggcaatc acgagttggc
    76801 agatgtgccc aatgaacaga tgatggacca ttttccagac ccacattttt taaatgaacg
    76861 gtatattgct ttaaatgagc agaccgttct tttagggatg aatggctggt acgactatca
    76921 gttttccgaa ttgaaagatt acgaggcaat cgtacgaatg aagcgtttgt attggtatga
    76981 tcggttcatc cagcgtggga aaacagatct cgaggtcaat gcagaaatgc ttgcagctgc
    77041 aaaaaatttg ctagaccagc tggaagcaaa aaaacttgag gtgattttat ccactcattt
    77101 cgttcctaaa cgggcattta tcatgtatca atctgctcct tatgagcgtt ggaacaaatt
    77161 aaatgcattt ttaggatcag aatcattcgg agaattactt gaccgttatt caaatatcaa
    77221 acaagtagtt tttggccata cgcatcgtcg ctttgaagat aaatccatcg gtcacacgat
    77281 ttacagctgc agaccttttg gttattatta tgaatggcag ctgacccgag attttgtctt
    77341 gaaagaacag ttgctagaca gctatgatcc tatgaaatta cgtgtcctct tgcgtgccca
    77401 tcacccagct tttcaggcct atcaaacaca acatttgcaa gaggaatttg aacaagcgat
    77461 gacaatcgtt ccgtattgag acagcattag taatgtgtaa aagaaaatac cttgtaaaac
    77521 ataaggataa gtaagagcag tagatccatt tatcgtggat atgctgcttt ttttgcggaa
    77581 aataatcagc gaatgtttat ttgatcggat gatttgtaac tcgcagagta ctagatgcaa
    77641 gcgtgtattc tttttttgaa cgataatcac aaaaaaaaat ataatcgtaa atttcaagtt
    77701 taagttagcc acccatggtg acccggttgc tcgttaccac caaccgtcta atttttctaa
    77761 tcgagtgggc ccttgacaat gagctgaggc atggtagtct cccgactggc tggccccggc
    77821 cagctttcca gacacaagcc atgccgaggg aggctcgttg tcaagggccc acgtgtcaag
    77881 ccgtcaaccg ttcctgccta aatgcctgga cgaacgtgtc atgcggcgtt gcatagccca
    77941 aaatctttcg cgggtaatcg ttcatccaac gctgaatccg caagatttgg ttttcgctca
    78001 cgttgccgat tgggctgccc ttggggatga aacggcgaat cagcttgtgt tggttttcgc
    78061 tcgtcccgcg ctcccaggaa gcataggggt ggctgaagta gatgtttgcc acatcctgca
    78121 aggcatcgtg cagtcccgcg aactccgagc cgttgtccga ggtaaccgtc ttgaacagct
    78181 ggccgaagta cgcaccagcc cgttctctca gctgtcggac cgcttggttg accgattcgg
    78241 catccttccc ggccagcttc agaatgacct cgaagcgtgt ctgccgttcc accaacgtca
    78301 gcagcacggc atccgacttg gtcttgtttc ctacgaccgt gtcgacttcc caatggccga
    78361 aggttttccg ggtaccaacc gcctccgggc gctcttcaat ggatttccct agcacccgtt
    78421 tattcgggcg gttgccatga cgggacgatt tcggtttgcg ggacagtttt tctagcaggt
    78481 ccatattctt cgttttcatg atgccacggt cgatccactg atagagggtg gtggtcccag
    78541 ggataatggc cgggtcgaac aattcgtgtt ttctggcaaa gcccaccacc acgtccgggg
    78601 accatttttc caacagcatc atgtcgtccg cccattcaat gaaggcgtcc gtgtcggccc
    78661 ataagggccg tctgccgcag ttcaagcggt tcttctcgta ggccgcctgc ccggcatcgg
    78721 cgtcgtagac gtcatgataa tagtcgtaga ccttcccgtt ctgattctgg cggttaagct
    78781 gagtaacggt cccgcgtttg atttcgttgt ttatggtctg cggcacgcag cccaggacat
    78841 ccgcaacctg acggttggaa tagttttcct ttttcaggat ggcgatttgg caacgttctg
    78901 agtaggttag gtgttttccc ttgcgtgaaa ctgtgttatc atggttttgc gtcatgtgaa
    78961 ttcatccttg tc
//
LOCUS       DACDRK010000006        68686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDRK010000006 DACDRK010000000
VERSION     DACDRK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.006"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(594..1985)
                     /locus_tag="IWJ77_000485"
     CDS             complement(594..1985)
                     /locus_tag="IWJ77_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9452135.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2198..2584)
                     /locus_tag="IWJ77_000486"
     CDS             complement(2198..2584)
                     /locus_tag="IWJ77_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9452136.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2640..3263)
                     /locus_tag="IWJ77_000487"
     CDS             complement(2640..3263)
                     /locus_tag="IWJ77_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9452137.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3327..3995)
                     /locus_tag="IWJ77_000488"
     CDS             complement(3327..3995)
                     /locus_tag="IWJ77_000488"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9452138.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4007..4447)
                     /locus_tag="IWJ77_000489"
     CDS             complement(4007..4447)
                     /locus_tag="IWJ77_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452139.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4506..5178)
                     /locus_tag="IWJ77_000490"
                     /pseudo
     CDS             complement(4506..5178)
                     /locus_tag="IWJ77_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5363..5761)
                     /locus_tag="IWJ77_000491"
     CDS             complement(5363..5761)
                     /locus_tag="IWJ77_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9452140.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5758..6531)
                     /locus_tag="IWJ77_000492"
     CDS             complement(5758..6531)
                     /locus_tag="IWJ77_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9452141.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6528..7211)
                     /locus_tag="IWJ77_000493"
     CDS             complement(6528..7211)
                     /locus_tag="IWJ77_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9452142.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7378..8265)
                     /locus_tag="IWJ77_000494"
     CDS             complement(7378..8265)
                     /locus_tag="IWJ77_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9452143.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8262..9131)
                     /locus_tag="IWJ77_000495"
     CDS             complement(8262..9131)
                     /locus_tag="IWJ77_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9452144.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9268..10554)
                     /locus_tag="IWJ77_000496"
     CDS             complement(9268..10554)
                     /locus_tag="IWJ77_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9452145.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10827..11405
                     /locus_tag="IWJ77_000497"
     CDS             10827..11405
                     /locus_tag="IWJ77_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9452146.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11405..12334
                     /locus_tag="IWJ77_000498"
     CDS             11405..12334
                     /locus_tag="IWJ77_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9452147.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12331..14067
                     /locus_tag="IWJ77_000499"
     CDS             12331..14067
                     /locus_tag="IWJ77_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9452148.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14130..14966)
                     /gene="pheA"
                     /locus_tag="IWJ77_000500"
     CDS             complement(14130..14966)
                     /gene="pheA"
                     /locus_tag="IWJ77_000500"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9452149.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14963..15472)
                     /locus_tag="IWJ77_000501"
     CDS             complement(14963..15472)
                     /locus_tag="IWJ77_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9452150.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15465..16760)
                     /gene="aroA"
                     /locus_tag="IWJ77_000502"
     CDS             complement(15465..16760)
                     /gene="aroA"
                     /locus_tag="IWJ77_000502"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9452151.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16773..17864)
                     /locus_tag="IWJ77_000503"
     CDS             complement(16773..17864)
                     /locus_tag="IWJ77_000503"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9452152.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17881..19047)
                     /gene="aroC"
                     /locus_tag="IWJ77_000504"
     CDS             complement(17881..19047)
                     /gene="aroC"
                     /locus_tag="IWJ77_000504"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9452153.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19049..20113)
                     /gene="aroB"
                     /locus_tag="IWJ77_000505"
     CDS             complement(19049..20113)
                     /gene="aroB"
                     /locus_tag="IWJ77_000505"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9452154.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20146..21168)
                     /gene="aroF"
                     /locus_tag="IWJ77_000506"
     CDS             complement(20146..21168)
                     /gene="aroF"
                     /locus_tag="IWJ77_000506"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9452155.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21827..22801)
                     /locus_tag="IWJ77_000507"
     CDS             complement(21827..22801)
                     /locus_tag="IWJ77_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9452156.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22782..24665)
                     /locus_tag="IWJ77_000508"
     CDS             complement(22782..24665)
                     /locus_tag="IWJ77_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9452157.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24683..26908)
                     /locus_tag="IWJ77_000509"
     CDS             complement(24683..26908)
                     /locus_tag="IWJ77_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9452158.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            27023..28057
                     /locus_tag="IWJ77_000510"
     CDS             27023..28057
                     /locus_tag="IWJ77_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9452159.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(28124..29137)
                     /locus_tag="IWJ77_000511"
     CDS             complement(28124..29137)
                     /locus_tag="IWJ77_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9452160.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(29215..29403)
                     /locus_tag="IWJ77_000512"
     CDS             complement(29215..29403)
                     /locus_tag="IWJ77_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9452161.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29843..29971)
                     /locus_tag="IWJ77_000513"
     CDS             complement(29843..29971)
                     /locus_tag="IWJ77_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9452162.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29974..31347)
                     /gene="rlmD"
                     /locus_tag="IWJ77_000514"
     CDS             complement(29974..31347)
                     /gene="rlmD"
                     /locus_tag="IWJ77_000514"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9452163.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31458..32513)
                     /locus_tag="IWJ77_000515"
     CDS             complement(31458..32513)
                     /locus_tag="IWJ77_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9452164.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32523..33953)
                     /gene="gatB"
                     /locus_tag="IWJ77_000516"
     CDS             complement(32523..33953)
                     /gene="gatB"
                     /locus_tag="IWJ77_000516"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9452165.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33950..35422)
                     /gene="gatA"
                     /locus_tag="IWJ77_000517"
     CDS             complement(33950..35422)
                     /gene="gatA"
                     /locus_tag="IWJ77_000517"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9452166.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(35422..35727)
                     /gene="gatC"
                     /locus_tag="IWJ77_000518"
     CDS             complement(35422..35727)
                     /gene="gatC"
                     /locus_tag="IWJ77_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9452167.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35833..37869)
                     /gene="ligA"
                     /locus_tag="IWJ77_000519"
     CDS             complement(35833..37869)
                     /gene="ligA"
                     /locus_tag="IWJ77_000519"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9452168.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37886..40120)
                     /gene="pcrA"
                     /locus_tag="IWJ77_000520"
     CDS             complement(37886..40120)
                     /gene="pcrA"
                     /locus_tag="IWJ77_000520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9452169.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            40275..41834
                     /locus_tag="IWJ77_000521"
     CDS             40275..41834
                     /locus_tag="IWJ77_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9452170.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41945..43876)
                     /locus_tag="IWJ77_000522"
     CDS             complement(41945..43876)
                     /locus_tag="IWJ77_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9452171.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43894..44808)
                     /gene="pfkB"
                     /locus_tag="IWJ77_000523"
     CDS             complement(43894..44808)
                     /gene="pfkB"
                     /locus_tag="IWJ77_000523"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9452172.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44805..45557)
                     /locus_tag="IWJ77_000524"
     CDS             complement(44805..45557)
                     /locus_tag="IWJ77_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9452173.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45716..45925)
                     /locus_tag="IWJ77_000525"
     CDS             complement(45716..45925)
                     /locus_tag="IWJ77_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452174.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45936..47264)
                     /locus_tag="IWJ77_000526"
     CDS             complement(45936..47264)
                     /locus_tag="IWJ77_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452175.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47629..48216)
                     /locus_tag="IWJ77_000527"
     CDS             complement(47629..48216)
                     /locus_tag="IWJ77_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9452176.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(48299..49549)
                     /gene="clpX"
                     /locus_tag="IWJ77_000528"
     CDS             complement(48299..49549)
                     /gene="clpX"
                     /locus_tag="IWJ77_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9452177.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49796..51085)
                     /locus_tag="IWJ77_000529"
     CDS             complement(49796..51085)
                     /locus_tag="IWJ77_000529"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9452178.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(51239..52171)
                     /locus_tag="IWJ77_000530"
     CDS             complement(51239..52171)
                     /locus_tag="IWJ77_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452179.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(52328..52987)
                     /locus_tag="IWJ77_000531"
     CDS             complement(52328..52987)
                     /locus_tag="IWJ77_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9452180.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52990..54015)
                     /gene="sppA"
                     /locus_tag="IWJ77_000532"
     CDS             complement(52990..54015)
                     /gene="sppA"
                     /locus_tag="IWJ77_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9452181.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(54028..54318)
                     /locus_tag="IWJ77_000533"
     CDS             complement(54028..54318)
                     /locus_tag="IWJ77_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452182.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54727..56532
                     /gene="glmS"
                     /locus_tag="IWJ77_000534"
     CDS             54727..56532
                     /gene="glmS"
                     /locus_tag="IWJ77_000534"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9452183.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56604..57959)
                     /locus_tag="IWJ77_000535"
     CDS             complement(56604..57959)
                     /locus_tag="IWJ77_000535"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9452184.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57983..59152)
                     /locus_tag="IWJ77_000536"
     CDS             complement(57983..59152)
                     /locus_tag="IWJ77_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452185.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(59149..60036)
                     /locus_tag="IWJ77_000537"
     CDS             complement(59149..60036)
                     /locus_tag="IWJ77_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9452186.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(60225..63917)
                     /gene="nifJ"
                     /locus_tag="IWJ77_000538"
     CDS             complement(60225..63917)
                     /gene="nifJ"
                     /locus_tag="IWJ77_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9452187.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            64116..64637
                     /locus_tag="IWJ77_000539"
     CDS             64116..64637
                     /locus_tag="IWJ77_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9452188.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(65336..65893)
                     /locus_tag="IWJ77_000540"
     CDS             complement(65336..65893)
                     /locus_tag="IWJ77_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452189.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(66072..66683)
                     /locus_tag="IWJ77_000541"
     CDS             complement(66072..66683)
                     /locus_tag="IWJ77_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452190.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(66796..67053)
                     /locus_tag="IWJ77_000542"
     CDS             complement(66796..67053)
                     /locus_tag="IWJ77_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452191.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(67650..67928)
                     /locus_tag="IWJ77_000543"
     CDS             complement(67650..67928)
                     /locus_tag="IWJ77_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452192.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(67962..68213)
                     /locus_tag="IWJ77_000544"
     CDS             complement(67962..68213)
                     /locus_tag="IWJ77_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452193.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        19211 a        14833 c        12080 g        22562 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttacgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgtaaaat atgaggaact atttttgttt
      421 ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttattc acggtgtttc
      481 agaagtagct tctcggaaat aagccgaaat ttcacaaaaa tttgagaaac tatttttagg
      541 aaacttcttc ttatttctta aagctgacca cttctatcac aacctctttt tgactatgca
      601 ttcacatcga tcactttaga atcatcattg atgacatcgc gatctaaatc tttgatgcgt
      661 gcacttgtca cgataccagc taccatgcta ccgctgacgt ttactgctgt acgtgccata
      721 tcgattaatg gttcaacaga gataactaac ccaacgattg ctactggcaa gttcattgcc
      781 cctaaaacga tcagtgaagc aaatgttgcg cccccaccga ctccagctac accaaaagaa
      841 ctgattgtta caattgccac aagcatcaaa ataaattcca aactaaagac attcactcca
      901 gctgctggtg cgacgatcgt tgccaacata gctggataaa cgccagcaca gccgttttgt
      961 ccaatagaca aaccaaaact tgctgagaaa ttagctgttg catcatctac tcctaaagct
     1021 tttgtctgag tctcgatatt caatggcaaa gctccagcac ttgaacgtga agtaaacgca
     1081 aagctcaata ccggaaaggc ttttttgaaa tagttgattg gattgacttt tgcaccaatc
     1141 aaaatcagtg agtggacaat caaaacagca agcaatgcta cgtaagaagc aacgacaaat
     1201 ttacctaaat tgacgatagc tgcaaaatca cttgttgcca aaacgtttgt catcaaagca
     1261 aatactccat aaggtgtcaa acgcaaaaca agcgtcacga tccgcataat gattttatac
     1321 agactgtcga ctaaatctga aaagaatgct gcttcttttg gcgcttttct tttcacgcct
     1381 agataagcga ttccgacaaa tgccgcgaaa ataacgactc caatcgtact tgttgcacga
     1441 ctacctgcta aatcagcaaa aacgttttta gggataaagg ataaaatctg ctgtggaatc
     1501 gataagtttt caactgtttc ctgacgtgtt gccagttcag agattcgtgc tgtttctgca
     1561 gctccttgtg tgaatttagc tccttggagt ccgaacagca ttgttgaacc aattccgatc
     1621 aatgctgcaa tagctgttgt tccaagtaaa gaagtcaata cagtcgcact aattttccct
     1681 agcttttcag aaactttcat ttttgtgaat gcaccaatga tcgaaataaa cactaaaggc
     1741 ataactagca tttgcaagaa tgatacatat ccttgaccta caatcccgat ccattccatt
     1801 gattgagtaa cgactttgtc gtctacacca aagatcaatt gaagcagacc gccaaagacg
     1861 ataccaactc ctaatgctgt aaagacgcgt gttgaaaatt ttgtatggcg cttttgcata
     1921 cgatagaaca catataataa tgccgcaaaa actagaacca ctaaaactgt gatgagtgtt
     1981 gtcatttttc tcctcctcat attattaatg acaagtccat cgaaggaagt aaaggttccc
     2041 gagaatgatt cttcaaccat cctcctgaaa aaaatttttt cagtgacttt tatattttag
     2101 acgattttta tcgaaatgac attctttttg cttacaatta gtaataaaaa aacatacctg
     2161 tgatagcttt aatctatcgc aggtatgttt ttactgtcta ttcttgtttt tctgattgaa
     2221 aattcaattt taccccaggc tgatctaagg cgatctctgt cacctcataa gacattcggc
     2281 gtacgatatc gaataacggt gctactagct cgtccacttc ttcttgtgtg acatcatttt
     2341 gaccttcaat tttccgattg aggatatgat tgatctgact cacacttcca gataaaacat
     2401 atgtatcaaa cactaaacgg aactctaagc gtgctcctac gatgctatgt tctttcggat
     2461 agttttcgat cgtttctttt aaaggcgaga agcctacttt caaatctgtt tctacttctt
     2521 ggttcggcaa tctcatatca taatgaaatg cttctacaac ttctttttgt cttctgattt
     2581 ccatataatt ttatcactcc tttatgaaaa ttataacata tctcctttga aaggggatac
     2641 tactttttaa tgatgctctg caaggtaagg atttcattca gatttcctac cacataatct
     2701 gccgggatct cacccagaaa atgatcgatg tcgtaaagac aagtagcgac tcctgcattt
     2761 ttccctgctt cgatatctaa acgacgatcg ccgatcatca tcgtgtctgt tctttctaaa
     2821 tgaaaagtat cgatcaaata atttaaagat gctggatcag gtttacgagg aaagtcctga
     2881 tcgatcccta ctacttcctc aatcagatgt gccaatcgat gttcttttaa caggccccat
     2941 gttgattctg tcaaacggtg ggtcaggatg aaatgacgac cgccgttgtc ttttagcatt
     3001 tccagtactt gtttcgtttc tggaaatgga cgggaaactt ccgtactttc tttttcatat
     3061 cggtgaaata attcattaaa ctctctggca ttcaagtttg attcattgat caaataacta
     3121 gtagaatact ttttcatttt gaagtagatt tcttttttgt ccatactgat accaaaatct
     3181 tttagtgctt gccatgcacc atctaccatt gcgggatacg tatcaaacaa tgtaccatca
     3241 aagtcccata tataattttt catgttcctt ttgttccatt cctttgcttc ttttttctat
     3301 ctctcacttt tagataaagg tgttctttaa agtatttttt tgtccagaat tgtcaaatca
     3361 tctgctaatg tatactggat tggttgtgca tttgggatat cgatcgtatc catttgatct
     3421 tcaggaacat cttctaaata ttttgtcagt gctcggatgc tattaccatg acccacaact
     3481 agtaaatctt gccgatcttt tagttttggt gctagatgat cttgccataa gggaatgatc
     3541 cgattcactg tcatttccag actttctccg tatgggatca aatgcggatc caagtgatca
     3601 tatctgcggt caaatctatt ttctgcggtt aacggcggaa cttccgtata tcctctgcgc
     3661 catgttttta cttgatcttt tccgtatatt tctttcatct tatctttgtt tttacccaca
     3721 agatccccat aatgccgttc atttagacgc catgttttta ttataggaag ccataactga
     3781 cccatttctt ctagtgcgac ttgtccggta agaatcgcac gtttcaaaac agaggtataa
     3841 acggcatcga attgaagtcc ggcttcaagc attttttgac cggcaactgc tgcttgttct
     3901 tttccttttt ctgttaaggc gacatctagc catcctgtcc agtaattttc aaaatttgct
     3961 tcgctttcgc catgacgtaa taatactaat ttcattcttt tctcccttaa acattcgttt
     4021 cgaattttat accacatgtt ttgcatgtca cacgtattcg tccttttcct ttaggtgcac
     4081 gctgcttttg atgacaattc gggcactcaa aaaaagtata actttttcga tccattttga
     4141 cttgcttttt gttttttatc tgatgatatt tttctttgac tctatcaaga tatttcaaat
     4201 aacgttgatt ctcatttagt ctggggtaga ttttttttga taggaaacga aatatcatcc
     4261 atccgaaaaa gacgatgaac aacaattgtc cgattctata gggcaaccaa cgacttagca
     4321 aactaaagac aagcgcagcg atcaatagac cgcgattcaa ctgatcaaat ttcgcatatc
     4381 ttccctgcat aagtttgtga tagttctgct gaaatcgtat tagcctttct aaccatgttt
     4441 gcatcatatt atctcctcca tcatctaata tttattatga caaaaaaatc caaaaagaaa
     4501 aagccttaat tggccttttc cattgcaact tggtgaagga cattccgatt tctggtaaag
     4561 tcaatcacaa tcactttctt tttatcttct ctgttgacta ctcgaaatgt gtccgtcaat
     4621 ggaagaaaca gcttcttaga tggtagctgt tgcatacgta taaaacgatg gatcatcgta
     4681 atcaattgag gaaattgaat agacgtgtga agcagacctg gatatttttt caacagctgt
     4741 gtcgttttga tcagattccg tttttttact gatcactttt aaaaagcttt ttagaatctt
     4801 ttgctgacgt ctaaaaatag aaaaagaatt ttcgtcatct atttgataac gcatttgttt
     4861 ttgcagttct gctgcagtca gatggatctt cccatcgttt gtatcgatca ttaaaccttc
     4921 tggaaacagt tcaaccactg ctcgatccca agattcaaga gaaagctcga tataatcttt
     4981 tactggtaat tccaattcta ccttaaaaaa gtgcaaaaca gccgacagcc cttctgcttg
     5041 atagatttct cctaatgtta gcccctctag atggaactct ggtgaaaaat gcagcaatgt
     5101 tttattttcc tgcaattgaa catatagaaa tgtagatcgc tcaatcagag tatcttttaa
     5161 aacaactaaa aaattcattt ttctccccct tttttcatca tttttaaaat agcatatttt
     5221 gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt cataagtatt
     5281 cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct tgtttgtgtt
     5341 tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta ttttcatcta
     5401 aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata ccaatgactt
     5461 tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg atgccttgac
     5521 caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac acaatatctt
     5581 tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca gaacctcttc
     5641 catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata acatcttcgc
     5701 ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca agatctttca
     5761 tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt catcttttcg
     5821 tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa tcattgcaac
     5881 cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga ttgccacgct
     5941 cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt tatcaaaagc
     6001 cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc cgtatcctgg
     6061 gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca atcctactgc
     6121 tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc tcccctcgct
     6181 caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc ctactaagaa
     6241 ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg caatagcact
     6301 gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga aaaggatcaa
     6361 cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt tgcgcatacg
     6421 aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg ccaaaatgac
     6481 ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca ttggaagatc
     6541 acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac cattccaaaa
     6601 ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc aagggccaag
     6661 ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc aatcggcata
     6721 aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat agccatcaat
     6781 tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa tactccagtg
     6841 ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga tatatcatat
     6901 acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat cccgatagca
     6961 aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt ccctactaga
     7021 tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat tccgatcatc
     7081 atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg tgcaaaaaca
     7141 aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa
     7201 ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt
     7261 cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc
     7321 aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta
     7381 ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc
     7441 agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga
     7501 agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac
     7561 taagggccat aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata
     7621 ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc
     7681 taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa
     7741 gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa
     7801 aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg
     7861 aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat
     7921 ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc
     7981 gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg
     8041 aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt
     8101 attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct
     8161 taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt
     8221 gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct
     8281 actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct
     8341 gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg
     8401 gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc
     8461 aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc
     8521 gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc
     8581 tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc
     8641 caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc
     8701 ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat
     8761 agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg
     8821 gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt
     8881 gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct
     8941 tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata
     9001 ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc
     9061 atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct
     9121 ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa
     9181 agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag
     9241 cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg
     9301 tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc
     9361 tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt
     9421 ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata
     9481 agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt
     9541 gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc
     9601 tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt
     9661 ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc
     9721 tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga
     9781 ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa
     9841 aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt
     9901 ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc
     9961 aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc
    10021 tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc
    10081 tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta
    10141 tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac
    10201 aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata
    10261 atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt
    10321 cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt
    10381 ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca
    10441 gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa
    10501 acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat
    10561 ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga
    10621 aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac
    10681 aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg
    10741 tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca
    10801 agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac
    10861 agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa
    10921 tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata
    10981 tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa
    11041 aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg
    11101 ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg
    11161 atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa
    11221 gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac
    11281 aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac
    11341 cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc
    11401 attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc
    11461 cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg
    11521 tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat
    11581 caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg
    11641 aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc
    11701 aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga
    11761 gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc
    11821 aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga
    11881 actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga
    11941 tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa
    12001 aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa
    12061 agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc
    12121 catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag
    12181 taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt
    12241 cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc
    12301 ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc
    12361 tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct
    12421 ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat
    12481 gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca
    12541 ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa
    12601 catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa
    12661 atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc
    12721 ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag
    12781 agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt
    12841 cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac
    12901 gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct
    12961 gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca
    13021 tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt
    13081 gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt
    13141 acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg
    13201 attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg
    13261 gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat
    13321 ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg
    13381 atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag
    13441 aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat
    13501 aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa
    13561 ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt
    13621 ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat
    13681 ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg
    13741 aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg
    13801 caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt
    13861 gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga
    13921 atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta
    13981 acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct
    14041 aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag
    14101 ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact
    14161 ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc
    14221 tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag
    14281 ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct
    14341 gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt
    14401 tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc
    14461 tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct
    14521 gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct
    14581 gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac
    14641 tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga
    14701 ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga
    14761 tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga
    14821 acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca
    14881 tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt
    14941 tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga
    15001 tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag
    15061 aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc
    15121 gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt
    15181 aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc
    15241 caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct gactccactt
    15301 ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt
    15361 cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac
    15421 cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct
    15481 cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct
    15541 tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata
    15601 ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg
    15661 atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc
    15721 accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg
    15781 attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga
    15841 atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc
    15901 gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca
    15961 tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc
    16021 ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt
    16081 atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg
    16141 atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca
    16201 acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc
    16261 gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg
    16321 attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt
    16381 tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta
    16441 aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg
    16501 tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc
    16561 tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa
    16621 tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg
    16681 atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt
    16741 atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta
    16801 ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc
    16861 agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc
    16921 gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata
    16981 aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt
    17041 tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct
    17101 gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg
    17161 ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga
    17221 aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga
    17281 ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca
    17341 tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt
    17401 ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat
    17461 agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg
    17521 acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca
    17581 tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag
    17641 gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa
    17701 tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga
    17761 ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa
    17821 gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt
    17881 ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga
    17941 atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc
    18001 aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg
    18061 ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat
    18121 gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt
    18181 attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga
    18241 gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat
    18301 actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc
    18361 taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt
    18421 tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg
    18481 tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc
    18541 tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag
    18601 aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg
    18661 ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg
    18721 tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac
    18781 agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga
    18841 accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt
    18901 catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc
    18961 ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg
    19021 ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct
    19081 ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt
    19141 ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta
    19201 gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc
    19261 cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta
    19321 ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg
    19381 tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt
    19441 ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt
    19501 cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga
    19561 tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat
    19621 caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat
    19681 tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt
    19741 gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca
    19801 ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat
    19861 cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa
    19921 ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag
    19981 caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg
    20041 atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat
    20101 gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg
    20161 aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt
    20221 ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac
    20281 gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca
    20341 aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa
    20401 tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg
    20461 ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca
    20521 ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac
    20581 agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta
    20641 ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa
    20701 ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata
    20761 tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc
    20821 cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt
    20881 cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg
    20941 gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc
    21001 atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac
    21061 tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac
    21121 tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc
    21181 tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac
    21241 attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact
    21301 tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac
    21361 atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt
    21421 ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt
    21481 tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat
    21541 tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg taagaattgt
    21601 acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt tttttaattt
    21661 tctgatagtt aactttgttt ttcagaaaaa taacaattga ttttattgaa gaaaatagtc
    21721 atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata gaataaagca
    21781 gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac tgaataataa
    21841 agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca tccgctcccc
    21901 agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc gttcgtcttt
    21961 ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg aatggtaaag
    22021 cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct tcttgatctt
    22081 tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt tccatatgca
    22141 tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact ttataaatac
    22201 cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct tcatactcaa
    22261 aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat tccggtaatc
    22321 ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg atttggtagg
    22381 aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg atttcaaccg
    22441 tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc aaatcgatct
    22501 ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg gctcctaacc
    22561 acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt gctcgccaaa
    22621 aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc gattccagtc
    22681 cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct aatgtacagt
    22741 cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact gtatttttca
    22801 taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa aacgatccca
    22861 ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata gcgcatcact
    22921 tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc ccatatatag
    22981 cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat tccacctaac
    23041 gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc tttattttca
    23101 aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc tgaaatcgaa
    23161 tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc ggggtctact
    23221 tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc atttccaaga
    23281 gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt tgctcgatca
    23341 agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg ctcaaatgcg
    23401 cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac atggattcct
    23461 gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt ccgtacgccg
    23521 ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat agtgatggcg
    23581 cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat ccgttttcca
    23641 ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga atagtcaata
    23701 tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata taaattgggc
    23761 tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt catttgctct
    23821 tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat cgctacattc
    23881 agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt gccattttga
    23941 catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg tcctcgagca
    24001 actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc ttccaagaat
    24061 gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc caaataaggt
    24121 gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa atgaccattc
    24181 aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg ttttcccttt
    24241 agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc tttagcagct
    24301 gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg gcgaaataca
    24361 tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt tgctaattca
    24421 atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt tttataaaat
    24481 gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc atttaaagac
    24541 tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac atgatcgcta
    24601 tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt tatatttgct
    24661 ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct gtttggaaat
    24721 caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc gaaaatatcc
    24781 gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga tatacttcat
    24841 ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct tcatcttgtt
    24901 taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc aaacgataat
    24961 aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg tcttttactt
    25021 gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa acagcgctca
    25081 tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga tttggtacag
    25141 ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca tattggattt
    25201 tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata taataaataa
    25261 acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt tccggataat
    25321 cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc gctacttctc
    25381 cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg ttcatatccc
    25441 atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc tcaatcgtct
    25501 gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca gaaggcattc
    25561 tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa tcaaccgaga
    25621 tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca taatctgcta
    25681 tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct aatgaagaat
    25741 tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg atgcctagct
    25801 cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag taagtggctt
    25861 cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt gcgattcttt
    25921 cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt gagtaagaaa
    25981 ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa aaacgatggc
    26041 tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt tcgaaagaat
    26101 ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct gtcgtatgat
    26161 gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga cggctttcaa
    26221 atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg acatgtgctc
    26281 ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc tggaaactag
    26341 ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca gatcgtgtga
    26401 tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct gtttctctat
    26461 ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa gtttgaggca
    26521 agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc agatccagta
    26581 agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc atgctgccat
    26641 gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga tttaagggaa
    26701 catttcctga aaatccacga tctcttcggg gataattttt attgtctttg tactgcttga
    26761 ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt atcagataag
    26821 aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag atgaaatttt
    26881 tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg aaaccgttta
    26941 ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa aaatttttta
    27001 tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa aggcaaatgt
    27061 ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg tggctcagac
    27121 tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca gtactcgtaa
    27181 atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca aagcgagcga
    27241 agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag ctcgacgtct
    27301 gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat gggaagatct
    27361 agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga ttaccgcctt
    27421 gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc aagaggtcga
    27481 tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt tcttagaaaa
    27541 tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg aattcggtgg
    27601 gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta tgatcgatca
    27661 tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac aaggtgaggt
    27721 acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc ttgtcgggag
    27781 cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc aaattggaga
    27841 agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct cccctggctt
    27901 gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa tggcattgga
    27961 gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac gattgattgt
    28021 tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg aaaagccgct
    28081 tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta tacttattca
    28141 caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa tcaaattggt
    28201 attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg ggtaccttta
    28261 attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat gcatttccgt
    28321 cagttatata cgttggcgga aattccttag aaacatggtc agccacacta gcttctttca
    28381 attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg gttttgacga
    28441 aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa tcaacaggtc
    28501 cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg attttcattt
    28561 gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt tgtgaacctg
    28621 cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct ttttcttcag
    28681 ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat tctgaatcag
    28741 gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact aattttgtcg
    28801 caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt acccaaaata
    28861 gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta ttctctttat
    28921 acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct tgatcgaacg
    28981 tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag aatgctccag
    29041 gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga atgattccga
    29101 agaaccatcc gataaattta agaattttct ttttcataag gctccctcag tagttagtat
    29161 taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga tgatttaatg
    29221 attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc attgttcttc
    29281 aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta agcacaaacg
    29341 atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct ggtgctcttc
    29401 catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct tcagagactt
    29461 tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa taggcacgga
    29521 cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat gccgccaaaa
    29581 tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt gagaatatga
    29641 aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa atctgagaca
    29701 gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc ttattggcaa
    29761 atcgccaaaa taatgtagac taactaaaaa gactaagaaa caataacaaa agttaacact
    29821 tcttagtctt tctttagcac gtctatttat taaagatttt agggcctttt ctcatgtttt
    29881 tacgatcatt cttaggtgtt tccatttgtt tggatcggcc tccaccttgt ttctttttga
    29941 tcaattcttt cattttttct ttttggtcca tttttattct cctttaacga atgaagcgac
    30001 taattcgata tgatgggttt gcgggaaaag atctacaggc tgaactttct tcacggcata
    30061 acctgcttct gcaaataact tagcatctct tgcaaaagtt gccggattac atgagatata
    30121 gacgattcgt tctggtgaca tttcgattgc tgattcaata aaatgatgat ctaatccttt
    30181 tcgtggaggg tcaactacga tcacagatgg tttgatccct ttttcttgcc attttttcat
    30241 aactttttct gcttttccga cttcataatg aacattttcg attttattat ttaaagcatt
    30301 gaactgtgca tcttcaattg cttctggaat aacttccatc ccatacacct tatccactct
    30361 gtctgctaaa gacaagccaa tcgtacctat tcctgaataa gcgtcgatca caatgtcttt
    30421 tttatctaac tcggcaaact cgatggctgt ttggtacaga tactctgctt gttgagtatt
    30481 tacttgataa aatgactttg ctgagattcg ataggttttt cctaatagct gatcttcgat
    30541 atagtctctg ccatataaaa ctttttcttt gtctcctaaa atcacatttg ttttttcttc
    30601 attgatattt tggatgaccg aaaccacttc tggcaattct tcatgaatag cttcagcaat
    30661 ttccttggat ttaaagaatt tttctttacg agtaactaaa acgaccatca tttcatggga
    30721 ataatgacca cgacggataa tgatgtgacg aagctgtcct tcattttttt cttcattata
    30781 tcctcgtact tggaaccgtt gcaagatatc tctgactact ttgattgctt gatcgatgac
    30841 aggatcttgg atataaaaat cttcgatagg gactagatca tggctattct ttttgtaaaa
    30901 accggtggct aactgtccat cgatacggcg aacagggatc tgtgctttat tacggtagcc
    30961 gaatggttct gccattccga ccgttggaag tacaggaact tctggcatct tagcaatctt
    31021 cgccattaca tgctctactt gtttttgttt gaagacaagc tgttgttcat acttcaaatg
    31081 ggctaaaggt gcgataccta tgcgaagtaa tgcattgttt tctacaggct gacgatcagg
    31141 tgattgtgta ataaatgttt cgacttttcc atatccaaac ttattgcctg cttttactac
    31201 tagtatttct acttcttctc cagggagtgc attttcgata aataatggat aaccatcgat
    31261 ttttgctacc cccattcctt cataactcaa gtcgacgatt tcgactgtat atcgttcgtt
    31321 tttctttact atttgctgtt ctgtcatatt ttgctccttt aagcctattt ttcaatccta
    31381 ctgaactcta ctataaatca gctaattaca caactaaaac cggcactgac cttactacgt
    31441 tcagcaccgg cttttgatta attcttttga ttaggtgttt cgtctgctat ttttccgtca
    31501 ccgtcgatat cttcatccgt taaacgttcg acttctttta cgaattcttt gctgacaacc
    31561 tctaattctt cttcatctgt acctgtgatt gcttcatcag gaatctgatc ggtattggca
    31621 aagaactcaa tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc gccgtattca
    31681 ccatctaagt tgatcattac tggtcgattg atatcatctt ctgtttcaac gaacaaactg
    31741 cttgttttgg tatagattat tcttggatca tcgacatgtc gaccgccatt cagcatgagt
    31801 gctgcaatat gcaagatctc aaagatattt gctgtcttca caatgattaa agaaaacttc
    31861 ccatcatcca atttggcatc tggcgcgatc tgttcgaatc ctccaacaga gttcgtcagc
    31921 cctaggaaaa acatcgaagc atttccgata tattcccctt catcatactt caagcgcatt
    31981 ttgattggct ttactcgagg gagcatctct gctcctttcg caagataagc aagataacca
    32041 aaaatactct ttagttctga tggaacttca taagtcaatt ctgtcaaata gccacctgca
    32101 gcgatattga tgaagtaact gttatcagaa aggccgatgt ccatttttac ggtttggtgt
    32161 ttttttatca cttctgctgc tgctttaata ttatctctag gaatttttaa tgctcgagca
    32221 tagtcgttcg ttgtaccagc tgggatgatg gccattttag gacggtgttc aagcggagcg
    32281 attccattaa ccacttcgtt gattgtgcca tcgccaccag ctgccactac tagatcaaaa
    32341 cctagttctg cgactctaca tgcttcattc ttggctgaat ctggttctgg tgtagttgca
    32401 taggcgctgg cttcaaatcc tgcttcttct aatgcctgta ggatatccgc caaatttttt
    32461 ttcaataatt cttttcccga ggtggggtta taaattacgc gtgctttttt cattttcagc
    32521 ctctatcgtt tcgctaattc atctttaagt agcttattca cgacaccagg atttgcttgt
    32581 cctttagttg ctttcatgat ttgaccgact aggaagccaa ccgcacgatc ttttccattt
    32641 ttgaaatcat cgacagattg ttggttgtta tccaaaattt cattaatgat tggcagtagt
    32701 ttgttcggat cagataattg cacccaacca tttttttcaa ctacttcttg ggcttctccg
    32761 ccatttgtga tcaattcacg gaagactttt ttggcgattt ttgagctgat cgttccatcg
    32821 gcgatcaaac gaatcattcc tgctaagttc tcaggtgtca attttgtctc agctaattcc
    32881 agtttctcac tgttcaagta agcagaaact tcacccatca accagttaga tgcttgcttg
    32941 gcatctgcac cattttccaa aacagcttca aaaaaatcag acatttcttt tgtaagtgtc
    33001 aataccattg cgtcatactc tggaagtcca aactcacgga tatagcgttc gcgacgagaa
    33061 gctggcatct caggcaaact agcttttact ttttgaatcc attcgtcatc gatcacgaat
    33121 ttcgggatat caggctctgg gaagtaacga taatcactag acccttcttt gacacgcatc
    33181 aaaactgttt tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat ttctcctcct
    33241 gaaagaagaa cttttgcttg acgtttttct tcaaaagcta aacctttttt cacgaaagat
    33301 aaagagttca agtttttcaa ttctgctttc gtaccgaact cttcttgtcc ataagggcga
    33361 agagaaatat tggcatcaca gcgcatagag ccttcttcca ttttcacatc gctgacttct
    33421 gtaaattgga taattgatcg caatgcttct agataagcgt atgcttcttc tggtgaacgc
    33481 atatcagctt cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa atcaacgtaa
    33541 gaataaccgc caataccatg catgtttttt ccagcatctt cttctaaatg gacacgttcg
    33601 atccggattt ttttcttttt accttctact tcgatttcga tccatccgtc atgaccgatt
    33661 ggttgatcaa attgggaaat ttgatatgct tttggattat ctgggtaaaa gtaatttttc
    33721 cggtcaaaat gagtatcttt agagatttca cagtttaatg ccaaagctgc tttcatcccg
    33781 aattccagcg cagctttatt cattactggc agcacgcctg gataacccca gtcgatcacg
    33841 tttgtattgc tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa aatttttgag
    33901 ttggttttta actcgacgtg gacttctagt ccaatgactg tttcaaaatt cattcgtctt
    33961 tccctcctaa aatagctggt tgttgtttat ggaattctgt tgcttgttca aacgcatatg
    34021 ccgcttgata catcgttttt tcatcaaaat aattaccgat gatctgcaat ccaactggta
    34081 atccttctga taaacctgcc ggtacagaca tacctggaag tccagccaag ttgacaggga
    34141 tcgttaagat gtcactcatg tacattgtga ttggatcgtt aatgttctca ccgattccga
    34201 atgcaactgt tggtgaagat ggcccgatga tcaagtcata atctgcaaag actttttcaa
    34261 aatctcgttt gatcaatgta cggacttgtc ctgctttttt gaagtaagcg tcataataac
    34321 ccgcgcttaa ggagaatgta cctaacatga tacggcgttt tacttcttca ccgaatcctt
    34381 ctgaacgtga gttgacatat acatcatcta atgatttcac attttcagag cgataaccgt
    34441 agcggatacc atcgaatcgt tgcaaattgg aacttgcttc tgatgaggca atgatataat
    34501 aaacagccac accgtatttt gaatgaggca agctgacttc ttccacaaca gcacctaatg
    34561 aacggaatgt ttctgctgct ttcaatacag cttcttttac gccaggggcc acaccttcgc
    34621 ctaagtattc tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc agtccctctg
    34681 caaaatcagg aactgaaacg cctgaagaag taccgtcttt ttcgtcataa ccgctgatag
    34741 ctgtcaatgc taatgcatta tctttcaccg tacgggtcat tggtccgatt tggtcaagag
    34801 atgaagcaaa ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt ttcatcccta
    34861 cgatgccgtt aaaagctgct ggttggcgga tacttccccc agtatcacta cctaaagaaa
    34921 ctggaatttg tccagctgca actgctgctg cagaaccacc agaagagcca ccaggtactt
    34981 ttgttgggtc ccaagcattt tttgttttct tgaaataaga agtttctgta cttccaccca
    35041 tagcaaattc atccatgttt agcttaccaa cagggatcat atctgcttga tagattttat
    35101 ccatgaccgt tgcatcataa attggttcaa aattgtaaag gatcttagat gcagcggttg
    35161 tcaagatatc ttttgttacg atattatcct tgataccaat tggaatacca gcaaggacgt
    35221 tgctttcatc aatccctttt tcatctaaag cttttgcctg ttccaatgct ttttcttcgt
    35281 tcaatgtaat gaatgcctcg acctcaggct ctgtttcttt gattcgttca aatgttgttt
    35341 ttgtcaaatc ttgtacagtg atttctttcg atacaagcat atgatgcagt tcttctagag
    35401 aacgattgtg taattctgtc attatgcgcc agcctcccca ttatcgatga ttgcgggtac
    35461 tttgataaag ccgtcttctg tttccggcac atttttcaat aattcatcgc ggttccatcc
    35521 agcttgcgcc cgatcttgtc gcatcacgtt tactgtttca accacattcg acgtaaatgg
    35581 aacaccttcc gtatctactt cttccagcat ttcaaccata tcgatgattt tacctaattg
    35641 gtcggtaaaa cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt tggcaacgtg
    35701 tttcacttgt tcttcactaa tcgccatact accccttctt tctaaacgag ttaaaaactt
    35761 tgctctctat tttaacacaa gagtgccgtg tctgaaattg tttattgaaa tttgtctgaa
    35821 aaaactcatt tttcattctt ctactgagcg acttttagcc aatgcatctg ccatttgatc
    35881 ttctgtccaa acttcgattc ctaattcttg tgctttggtc agcttgctgc cagcatcttc
    35941 tcctgctacc acgatatccg tttttttcga tacgctacca gtcactttcc cgccaagatt
    36001 ttcgatcgtc tcttttgctt cttcacgagt aaatcgtgtt agtttaccag taaggactac
    36061 tgttttttct ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa gccctttgta
    36121 ttcgaaattg acgcctgctt gttttaattc attcatcaat tcatggactt cttcgttact
    36181 gaaataagtg actacactgt cggcaatgat atcaccgatc gtatcaagcg caataatcga
    36241 ctcatagtca cttttactca atgtttctag atcaccgaaa tgttctgcta aaatttttgc
    36301 tgctttcgct ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga ccgaattatc
    36361 tttgctgttg tcgattgctg tcaaaatatt atttgctgat ttttctttta ttttgtccaa
    36421 cgtcaataac tcttcttctg tcaatttgta aagatcagca acatctgcta ctagtttttt
    36481 atcatacatc tgttctagta cgcgcggccc caacccatcg atattcatcg catttcttga
    36541 aacaaagtgg ttcaaacctt ctttcatttg agcagggcat ttaggattga tgcaccgtaa
    36601 ggctacttct tcatccaaat ggacaagttc acttccacaa actggacaat gtgtaggcaa
    36661 ttggtattct tcgctgtctt taggtcgttt atctaacacg acttgcgaaa cttctgggat
    36721 gatgtctccc gctttataaa tcagaactgt atctttcaag cggatatcct tcaacttaat
    36781 ataatcaccg ttatgcaggc tggcacggct tactgtcgtt cctgctactc ggactggtgt
    36841 catgatagct gttggcgtta ctacccctgt tcgaccgact gtccattcga tgttttcgat
    36901 tagcgtttca acttcttctg gcggaaactt gtaagctgct gcccatctcg gtgctttgat
    36961 agtaaaacca agttgatctt gtagcgagaa ttcattcact ttgatgacga ttccatcgat
    37021 ttcatagggc aaatccacac gtttgtcatg atattcttca atgtatgacc acacttcatc
    37081 aatcgaatga catagacgtt tttcccgatt cgtgtggaat ccaatttttt ctagctcttc
    37141 tagcgcatca tactgggttt ttgcttgcat aggaccgaaa tcagctactg tgtataaaaa
    37201 agtgtccaga tttcttttcg ctgtgatttt agaatctaac tgacgcaaag aaccagctgc
    37261 ggcgttccga ggattcgcaa agatatcttt tccctctgct tctctgtctt ggttcaattg
    37321 gacaaatgac cgttttggca tgaaacattc gcctcttact tcgatgttca ttggttcttt
    37381 taatttgatt gggaccgacc ggacagtttt taggttttca gtgatgtttt ctcctacagt
    37441 tccatccccg cgagtcgcac ctctcacgaa atttccatct tcataacgca aagaaacgga
    37501 aagtccatct atttttagtt cacagcaata gtcaactaca cgtcccactg ctttttgtac
    37561 acgttgatca aaagctatca gctcttcttt gctgaaaacg tcgttcaaac tgtaaagcgg
    37621 aatatcatga gtgacttttt caaaaccttc taatactttt ccgcctactc gttgagtggg
    37681 agaatcagac gtgatcaaat caggatattg ttcctcgatt gctactaatt ctgcgtattc
    37741 tttatcataa acatagtctt ccactgttgg tttatcttct acgtagtatt cgcgagacca
    37801 ttggttcagt cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa tcgtcatttc
    37861 atgttccatc atttaccctc cactactaag ctttttcaat tggcgcaaag gcagccaaaa
    37921 gacgttttac tccttgactt gggaaagcca catctagttc tagatccttc gacgtcccgc
    37981 ttactcggac gactgttcct agaccccatt ttttgtgttt gactttatct ccaggtttcc
    38041 agctgttatt ttcccctcca gtagcacttt tagagggaac aaccgctgtc tctggctgtt
    38101 tatagctata tgctgttttt ttagcgaata caggcgtttt tttaggcgta ggacgcatgc
    38161 cttcgatttc cagtaactcc tgatcgattt cttctacaaa acgagaaggt cggttatatt
    38221 gcgtccgtcc atataatgtt ctggagaaag catttgttaa atacaacgct tcttctgctc
    38281 gcgtgatccc cacatatgcg agacggcgtt cttcctccag ttcgctttct tccatcaatg
    38341 ctcgagataa aggaaaaacc ccctcttcta gaccaatcag gaatacaact ggaaattcta
    38401 atcctttggc tgcatgcaaa gtcatcaatg tcacttggga agcatcttct tctaaattgt
    38461 caatgtcgga aacaagtgcc agatcattta agaagaccgt tagtttttct tctggtgcat
    38521 cagcgtcttc ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg accgttaaaa
    38581 attcctccaa gttttcgatg cgtgcttgtg cttctaatgt attttggatt tttaagtctt
    38641 ctagatatcc gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc aagtatggga
    38701 tcatctgagt gacttcttgg atcatctctc caaatgcacc taattgctgt cctgcttttc
    38761 caccgatatt tgccagatcc acattttgcg ctgcttcaag cagtggccat tcatgaagag
    38821 aagcaaaact gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg ggcgaattaa
    38881 cgatacgctc aaaactgatt gaatcttgag gatttaccag cacattcaaa tacgctaaaa
    38941 tatctttgat ttctttacga tcgtagaact tgtgccctcc aaccatttta taaggaatat
    39001 ttgctttcaa cagagtttct tccattacac gagattgtgc gttcgtccgg tacaagatgg
    39061 caaagtcgcc gtaattccga tgattgcttc gaatctcttc ttgcatccgg tctacgataa
    39121 aacgggtttc gtctcgttca ttatctgcac ggtagtaagt gatcttgttt ccttctttgt
    39181 tttctgtcca caagtttttt ggttttcgat tgctattgtt ttcaatgact tggtttgctg
    39241 cgctaaggat gtttttcgtg gagcgatagt tttgttctag caaaattact gcagcatctg
    39301 gataatcttt ttcaaaatcc aagatgttct gcatatctgc accgcgccag ccgtaaatac
    39361 tttgatctgc atctcccacg acgcacaggt tcctaaatct acctgccaat aaattgacta
    39421 gcgtatactg tgcgtgattt gtatcttggt actcatctac atgaatataa tggaatttgt
    39481 tttgataata cgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta ttcatgatca
    39541 ggtcgtcaaa gtccatacat tgattatatt gaagctcttt ttggtaagca gcataacatt
    39601 ttgccgcaat ctcttcgtaa taactgcctt gcatttttgc aaaatcctga ggggtctgca
    39661 ggctgttttt tgcttgagag attgttccta atatacttcg aggatcatat tttttcggat
    39721 caatgttcag ttcttttagt atccgtttca tcaaggtcag ctgttcagaa gaatcgatga
    39781 ttgtaaagtt ccggttatac ccgataaaat caacatctct tcttaaaata cgcacacaca
    39841 tcgagtgaaa agtcgatacc cagacatctt cgcctccaga tgcgagaatc gcattgacac
    39901 gttctttcat ttcttttgca gccttattgg taaaagtaat cgctaggatg ttccaaggat
    39961 tgacttcttt ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc gtctttccac
    40021 ttcctgcacc tgccattagc agtaatggtc cgtcagtgtg aagaacagct tctttctgac
    40081 gggggttcat cccgtttaat aattcagctt tggatgacat taacaacatc ctttctttta
    40141 tcaatcttgt taattatagc attttcttgg ctttcattaa actagctagt tcaaaatcat
    40201 ttgtttcttt ttatggattt ttccttgtca attcgttgat ttcgcgctaa attaaaagaa
    40261 agcgaggcgt ttgaatggaa aaaaaaggaa aaatcaagaa attagataag actcagatat
    40321 attttattct aaaaggaata tttgttggac tgctgacagg aatcgtcgtt agcctgtttc
    40381 gattatctat cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg agtcgggaac
    40441 agccgattta cttaattggc ctagctattt gctgcatcct tgcagcattt ttcgtgggtt
    40501 atctggtcaa aaatgaaccg gatatcaagg gaagcgggat tccacaagta gagggacagc
    40561 ttcgcgggga attatcaatg aactggttct ctgttttatg gaaaaagttc attggcggtg
    40621 tactttcagt tggtgcgggc ctttttctag gtagagaagg accttctata cagcttggcg
    40681 catccattgg acaaggagct ggacaattgt ttcggagtcc atcttcagaa gaaaagattt
    40741 taatttctag cggtgcaagt agcggacttg cagcagcatt caatgctcct atcgcaggat
    40801 tactctttgt tttagaagaa atccatcact ccttttctcc acttgtttgg ctgacatcat
    40861 ttgcttcagc aatcactgct aacttcgtct ctctttactt tttcggcttg agacctgttt
    40921 tatatctagg tgaccttcct tctttgccgt tgaaatatta tggttcctta gtgatcttag
    40981 gagtacttct aggcatctta ggttttattt atcagaatgt cttgctggct ttgccaaaat
    41041 tttacaaccg attgccttta ccagcttatt ttcacgggtt cgttcctttc ttgctgattt
    41101 tgccaattgg ttatttatgg cctagtactc taggcggagg caatcaaatc gttttagctt
    41161 ttggacatgc ttccttgcca atcattatga ttatcggttt gtttgttctt cgattcgttt
    41221 tttccatgat ttcttacggt gggggtcttc caggggggat tttccttccg atattgacat
    41281 tgggggcttt aatcggtagc ttttatggaa acattatggt tgatttcggg atggatccaa
    41341 tctacatcaa ggattttgtc gttattgcca tggctggtta tttcacagcg attggaaaag
    41401 caccgctgac agcaattatt ttagtgacag aaatggttgg ctctcttaat catctgatgc
    41461 ctttaggatt ggctgctctt gttgcttata tcgtcaatga ccttctcggc ggtaatccaa
    41521 tttacgaatc attattggaa cgtctacttg gaaatactca gaccgcctca ctaaaaggtg
    41581 caaggacaac ttttgatttt ccagtaacgg cagagagttc tttagacgga ttaatggtac
    41641 gagacttcaa ttggcctaaa aatatgctgc taatctctat acgcagagga aatcaggaaa
    41701 tcttgactca cggtgatacg atcatgaatg taggagatat tttggttatc ttgactgatg
    41761 aagcaaactt gccaagaatc aagcaggaaa tcgaggctaa atcttctatc acaaatttga
    41821 agcaatcatt ttaacgaaca tggaaaaact attttacaac aactattcat ctacactact
    41881 tgattctata tgattctata aaaaaagaag aggctgggaa aacccagcct cttctttttt
    41941 atttctatgc ttctgctact gcaggagctt ttctcaataa accataaatc acaccagata
    42001 cgactgctcc gatagcaata aacaccaaat acatgaccgg gtgactcagt aagaatacaa
    42061 cgaaaatccc gccgtgagga gccagtaatt tgattccgaa agctccgact aatccgccag
    42121 ttaaagcaga accaataata aaacttggaa tggcacgaat cgggtcagct gctgcaaatg
    42181 gaatcgatcc ttccgtgaca aacgatagtc ccatcacgat gttcgtcaaa ccagcatctt
    42241 gatctgtttt gctgaattta ttcttaaaca aacgtgtagc aacgaaaatt gccaatggag
    42301 gtaccattcc tccagccata acagaagcca tcacgacact tccgccagtt gcaacagtcg
    42361 tagctaaagt tgctgtacca aacacataag cggctttgtt gattggaccg cctaaatctg
    42421 ccgccatcat tccagctaat aatgcaccaa gtaaagcagc attcgaacca ctcatacttg
    42481 tcaagaaacc gttcaatgca tcattgattg ctttcatagg gacattgacc agcaacatca
    42541 agaaaccagt aatcagtaag ccaaacactg gataaaagag gatcgtttta attccatcca
    42601 atgattttgg taagttcttg aataatttac gtaaaaatac gatgacataa ccagctaaga
    42661 atccaccaat cagtgcacct aagaatcctg caccgcctgt attagctaat gcaccagcag
    42721 caaatccaac gatcaaacca gggcggtcac cgatacttga agcaataaat cctgctaata
    42781 ccggcagcat gaaaccaaat gcagcttgtc cgatttggtt gaaccagcta gcagcttgat
    42841 tgtaattccc tagatttgat aattgatcat gtggcacgcc aataaattgg tcgatcatga
    42901 aagaaagcgc aatagcgatt cctccaccaa taacgaaagg taacatgtgt gatacgccgt
    42961 tcatcaaatc tttgtaaatt ctttgtccga tagtaccatc cgcatttcct tcttctgaac
    43021 tgctttcttt tccatcacca tggaatactg gtgcagtacc actaagggct agattgatca
    43081 actcttctgg tttacggata ccatcactga caggacgatt gatcagttct tttccgtcaa
    43141 agcgattcat ctctactttt ttgtctgctg caacgattac accttctgca cgggcgatgt
    43201 cttcagcagt caatcggttt ttgattcctt ctgaaccgtt cgtttcgact ttgatctcca
    43261 cacccatttc tttcgctttt ttcttcaaag catcttctgc catgtaagtg tgagcgatcc
    43321 ctgttggaca agctgtaacg gcaacaacga attttctttc tggatgagca gaagcttctg
    43381 cagcttgtgt ttcttgttct gctttttctt ctgcttcttt ttgctcttca gctgtttgga
    43441 acaagtcttg gacttcttct ggtgtatttg cttcttttaa ttttcctacg aaatctgcat
    43501 caatcagcaa tcgagatagt gctgctaatg cttgcaaatg tgtgtcgttt gcgccttctg
    43561 gagctgcgat catgaaaaac agataagttg gctgtccgtc taaggcttca taatctactc
    43621 ctttttggct ttttgcgaat aaaactgttg cttctttaac tgcactgttt ttcgcgtgag
    43681 gcatggcgat tccatcacca agacctgtag aagtttgtgc ttcgcgggct agaattcctt
    43741 ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa cattttttgg accatttcat
    43801 cgattgcgcc ttttttatca gtcgcttgca aatccatgat catcgcttct ttgaccatta
    43861 aatccttgat attcatgatt atcctccttg aaactaatca atttttgtga tttcaacttc
    43921 tggcagcaac tcatcaatca attcgcgagc tgccaagtca tcagaaaaag ctgttgcgct
    43981 tccgcaagca actccccatt taaaggcttc gattggatct ttcgtacggc tgtaagcccc
    44041 aataaagcca gcaatcattg aatcccctgc acctactgaa tttttcaatg ggcgaactaa
    44101 tacattcgtt ttataagttc cttgttccgt gaagagcaaa gcaccgtctc cagccattga
    44161 gatcagcaca ttctgtgccc cttcagctag taattttttc ccatacggaa tgatttcttc
    44221 tacagattgg aaggttgtgt tgtaaagctc agctaattcg tgattgtttg gtttgatcaa
    44281 caacggtttc ttaacaagtg cattttttag gtcatcacct gttgtatcaa tcacgaaatc
    44341 tgcacctttt tctcgaatga tatcaatcag ttcttgataa aatccttttc gcaggctagc
    44401 tggcgcgctt cctgacaata cgacgatatc tccttgtcct actgttccta atgcttgctt
    44461 caactcatcg atttcttcat ctgaaataga tgggcctaag ccattgatct cagtttcagt
    44521 gtctgatttt agtttgatat tgattcttgt atcttctttt acggttgtga acaaagactg
    44581 gatttgttct ttttcaagcc acttcgaaat aaattcgcca gtgaaccctc ctaaaaaccc
    44641 tagtgctgta gattgagaat cgatccgttt caaaatacgg gaaacattga tcccttttcc
    44701 tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg cctagtttta acccatctac
    44761 gcgtacgata taatcaattg atggattaag tgttactgtg taaatcattg tattgcctcc
    44821 ttaattgttg tcttttcacg gaattcatta acgaattcta atggacaacg ctcggttatg
    44881 atcgtggctg cttccaaagg tgcaacagag gtgaaggaca actggttgaa tttggtatga
    44941 tcaatcaata caaatgtctc ttcggaattt ttcatggcta aacgtttaac agctgcctct
    45001 tctggatccg gagtcgtgaa tccttgttca agatgggcac cgttcattcc cataaatgct
    45061 ttattaaaat gaaaattttt tagttgttct atcgcattag atcctaaaac agcattagtt
    45121 gccagcttta tcgttccacc taaaataatg gtagcaagct ccaaatcaac taaatttgcc
    45181 gcatgttgaa cggaatttgt cactactgtg atctgttttc cttgcagaaa aggaatcatt
    45241 tctaacgtag tagagccagc gtctaaataa atgacatctt cgtcattaat ggtttcagct
    45301 gccgatttgg cgatttgctg tttttcgtga acgtttttga gtgatttttc actcatattc
    45361 tgctcaaaat tcaataattg ctctttctta gctccgccat gaactctttc tagaaaccca
    45421 ctattctcca gctcttgtaa atcacgtctt atcgttgatt cagaagcatt caaaaggttt
    45481 acaagatctt gtgatttcac gattttctgc tgttcaagca agtctaggat tttttgtctg
    45541 cgttcctctg taagcatttt cacgatcacc tcaatacaaa taatagcata ttttttttct
    45601 actagcaata taaatcagtc aaaaacgttc ataaaaatta taaatcagtc aaaaaaaaat
    45661 aaaaaaccag tcagaaagac tggtttttta tttttttgtc tatcatcaag ccgttttatc
    45721 gttttttatt ttcgcttctt gtttttttca tataaacaga agcaggtttt ctttccttat
    45781 gaagaagata ataataaagt cccatcccgc caaacatgac caaaaaaccg atgactgatc
    45841 gaataggaaa attaggaaca aaaaagagac agacggataa taatgatgcc ggaaccatta
    45901 ttcgataata gagttgttta tccattttct tcacctcata ttttttgtaa agtaatcaat
    45961 tgttctaatt ctcggtaatc taaaaattgc acacgcagat gctgctcacc tttcttgaca
    46021 tcgaattgga tacttcgccg ctttttcttt attcgtttga aggtaacatc agagagtcta
    46081 tgtttgattt cctttaacgg taattctgac agggattctg aaggaacata gattcttaag
    46141 gataaaagat actcttgata tttttcatct gttacctcta cttctagata ttccctaata
    46201 taataacaat aagcttcgat tccattatcc aaagcaacag tcggatcgat aactttatag
    46261 cacatcatgc gtgatcgatt ctcgtcaacc caaggaaaat acgcctgagc atttagtgtt
    46321 ccgcgaacac gcctttcttt ataatctcct tgttggatat cataactttt tgttacaaaa
    46381 aatgaagcat tctttttagg aacaggttgc atttgtgcat tctgatactg gacagattgg
    46441 atcgtatggt cagtgtaaat gtattcaacg aatggcggtt tcaataaacc gacaccattg
    46501 tcccaagcaa gatgatcaaa cccaagctgg tccctaaaat aggattgaaa catttgattg
    46561 tttggctttt gccagttaaa ggggtctcga taaagctgga tacatatgac ttcgttttct
    46621 aattccttca ctttttctac ttgttggatg tcgagaaaaa tcggtttagg gatgatttga
    46681 tgataatagc gactataata catttcccaa cacgaagtga aacataaacc ttggtaaaaa
    46741 agatcgtatc ctggaaattg gttgcaatcg actactactt ccccgttttg ttgtttcatt
    46801 ttaggtaaat gttcgatttc atcctttttc tcaaacaata gccgttgaga gatctgtttt
    46861 gtattgtgat agataaactc gctataagct cgcatatagg cgaacacccc atgagcaatc
    46921 atttttttct gaatgaagtc tcgaatcaaa aattgctttt ctttgaaaac agagatagac
    46981 aatacatttt ttatcaataa ttgattatct tccaagcgaa aactacattc gttctcgtca
    47041 tttcctacaa ctaaccaact ttcgtctctt accgatgcta gcattatttt ttcttgaagc
    47101 tttcgaagat catacggatc cgcttgttgt gtgaaagagc ggataaatgt tggctggatc
    47161 aacagacgtg tcaaaatagc ccaatcgctt atttcctgtt ttttttcttc ttcatttaag
    47221 tctgtgagct ggatagtcat caataattta tcttcgccaa tcattttctc acctctttca
    47281 aatcttttta taatttaatt ataaccaatg ataaagaaaa actaaaactt tatgtcttag
    47341 aaaccaattt ggacgattac atgatagact aagtaaaaag aaacaaagag gtgtttacaa
    47401 tggggttgaa atttgaacgt acagataatt tagataaact tttcgaatca tttgctgtcg
    47461 atccggaaaa aacagttgca ccaaaagaag agatcgatcg atttctaaaa ccacagccac
    47521 aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa gctcaaaaaa agacttacca
    47581 gccaaacttt tatgaaagag tttgactggt aagtcttttt tattcttttt atgattcaat
    47641 tgcttgttcg attgctgccc acgcttcttc ttttccctgt tttgtaacgg aagaaaagag
    47701 aataaacgtg tcattgcgat caaaatccaa cgcttttttg atttcgctct catgcttgtt
    47761 ccatttacca cgagggatct tatctgcctt agtggcaaca acaattacag ggatatcata
    47821 atacttcaaa aattgataca tctggatatc ttcttgagaa ggcttatgac gcaaatctac
    47881 taaagaaacg accgcacgta gctggtctct agtcgttaaa tatgtctcga tcatctttcc
    47941 ccattttgct cgctcagttt tggatacttt tgcataccca tatcccggaa catcgacaaa
    48001 atgtaatgcg ttctcgatca aataaaaatt caatgtctgg gtctttcctg gcttaccgga
    48061 agtacgggct aaattctttc gatcaatcaa tgtgttgata aaagaagatt tccctacatt
    48121 tgatcgtcct gccagagcta tttctggcaa ttgtgtatct ggatattgtt ttggtgccac
    48181 tgcacttatg ataatttcag cttgatggac tttcatggtc tctctcctta acaaggtgat
    48241 gaataaagca agcggctgtc gcaaaagcca aaatgacttt tacggcagcc tcttttcatc
    48301 aacccgcttt tttctctgaa tctttataga ttactgttgg ctcaccagac aattcagctg
    48361 cttcttttgt gataatcact ttttcgatgt tttcatttga agggatatca aacatcacat
    48421 ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc accggtattg cgttcgattg
    48481 cttttttcgc aatggctctc aacgcttcag gttcgaattc aagttcggta ttatctagag
    48541 aaagaagttt ttgatattgt ttgactagcg catttttcgg ctcagtcaag atccgtacca
    48601 gatcatctgt tgtcaatttc tctaaagctg ccataacagg taatcgtccg ataaactcag
    48661 ggatcaatcc gaattttagt aaatcttcag gaatgatatg ctgcatgaca ctttcgtctt
    48721 cagaaagttt ttggttttgt gtaccaaatc caatcgtctt ttcgcccata cgattcttca
    48781 caatcgtttc gatgccatca aatgcaccac ctacgatgaa caatatgttc gtcgtatcaa
    48841 tctgaataaa ttcttgatgt ggatgtttac gtccgccctg aggtggcaca cttgcaacag
    48901 ttccttctag aattttcagc aaggcttgtt ggacaccttc gccggaaacg tcacgagtaa
    48961 tagatacatt ttcgctttta cgcgcaattt tatcgatctc atcaatatag atgatccctt
    49021 tttctgcaag ttccacattg taatcagcag attgaagcaa tttaagcaaa atgttttcta
    49081 catcttcccc cacataacca gcttctgtca aacttgtcgc atctgcgatt gcaaaaggta
    49141 cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt accagaccct gtaggtccga
    49201 tcaagcagat attgcttttt tgcagttcaa catcatctgt gttttcttgt gcttgctgat
    49261 tgacacgttt atagtgattg tatacggcaa cagctaacga gcgttttgcg cgatcttgtc
    49321 caatcacgta gttattaaat acttcaagaa tttctagtgg tttagggact tcagtgaatt
    49381 cgcgtacagc ttcttcataa aattcttcat caatgatttc tttacatagg tcgatacatt
    49441 cattacagat gtagacccct ggaccagcta cgatcttttt cacttcttct tgggttttcc
    49501 cgcaaaaaga acagcgtacg gtctcattgc tacttggatt gtcatacatg gtcttcaccc
    49561 cttaacttgc aaaaagtatg caagaaacta tcaaagactt tacagtcttt tactatcata
    49621 acatagttta gtaaaaagaa aaagtgttta acaaaggatc aactgaccta tacctttgca
    49681 ttttttactc ttaacaaaaa ataaggaact gtgacaaaat cgtgtcacag tcccttattt
    49741 cgaataaaca attttctaga agtagcttct tcagaaatga tttattactt gatttctatt
    49801 ctttcgcttc tgtcgctgta cctgtgatca attcgattgc ttttttcatt gtcacatcat
    49861 gttccagcat atcttctgtc aacacgcgtt tgatttgttc gattggcatg ttgtatgttt
    49921 ctgataattc ttggatttct gcattgattt cttcttctgt tgcttgaaga ttttcagcag
    49981 ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat ttctgcttca ccttcgaatt
    50041 gtttatgcaa gtcttcttct gtagaaccag ttaattgata gtacatttca ggagagatac
    50101 cttgacgttg catgttgttc aagaattcgt ccattgaacg atgtacttca tcatgaacca
    50161 tcacgtgtgg caattcaacg atttctgcgt tttcaacagc catacggatc gctgcttcat
    50221 cttttgcatc gtccgctgct ttttcttttg tttctactaa ttctttgcga tatttttctt
    50281 tcaattcgtc taatgttgat acttcgtcat caacatcttt tgcgaattcg tcatctaatt
    50341 caggcacttc ttttgctttt acttcatgga ttgtcacttt gaagactgct tcttttccag
    50401 caagatcttc tgcttgataa tcttctggga aagttacatt tacatctaga ctttcacctg
    50461 atttatgacc aactaattga tcttcaaaac ctggaatgaa tgagcctgaa cctaattcaa
    50521 gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc atctacaaag ccttcgaagt
    50581 cgattactac tgtatcgcca ttttcagctg cagcatcttc tttgataact aattcagctt
    50641 gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc atctgttact tcacggtctt
    50701 gtttttcaac aactagatct ttatattgtc ctaattttac ttctggtttc actgttactt
    50761 cagcagtgat tacccagtct tgaccttttt ccatgctttc aacatcgatt tttggttgag
    50821 aaacaggatc gatgccagct tctttgacag cagcttcata agcttctggt aatacagcat
    50881 tcaatgcatc ttcatataaa gcttcttcac cgtacatacg gttgaatact tgacgagaaa
    50941 ctttcccttt acggaatcca ggtacgttca agcttttttt cactttatta aacgcagttg
    51001 ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt taaaacacca tcattcgtgc
    51061 cttttttttc aaatgttgca gtcatttaat tccctccgag tttagaaatt cattttatac
    51121 atcaaaaacg tttcatgcat acctttaaat agtacactaa ccctttcaaa ttgtaaacat
    51181 attgaatgga tagaatcctt gtttttcata acatttttat ctttttcact gataaaatct
    51241 aatggaagaa gatcatttgc tgcaaaattt tttctctttt ttttaaaaaa tcttgttttt
    51301 catcttctgt accttcatac atcgtttttc ctgtacacgc ctctacagtc agttgaaacc
    51361 agcgcttcgg gtcttcgatg tatgtatcat agacaggata aacaattgca gattgcaacg
    51421 tgaattcttc ttttaacacg gaactaagca gcgcatcttc attcccgtaa cgatctgaaa
    51481 atacacataa ttgctggtaa cttgcctgct gctgcggctt tggcagatct tttggtatga
    51541 ctttgactaa cttttcttca atcgtcaaat agaaaacagg atggcattcg ttcaattgcc
    51601 taagtgtctc cagtaatttt gcccttgcta aaatatggac ctctggcgat acaataagtt
    51661 tactcgctgt ttggataaac tcatgataag gaagctgttc gatctcatca atagacgcaa
    51721 gctgacgtgc aggttccaac gaaggaagtt cctccgattt attgtggagt gccttgatct
    51781 tctgcctctc catttgtccg gataattctt ctgcaaattg gattttttca atgacccgct
    51841 gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt tctagcatat aaaaaatctt
    51901 gtgtactgat taacaagtca aaataaaatt cagcaaactc ctcattttga agatactgtt
    51961 tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc gtccaactcg taaagacaaa
    52021 aaacaagcag gctattagcc tcaaagcttt ctttcaactc gtaagctcgt tgaaataatt
    52081 cttttgcctg ctgatattgc tggtttgcta gtgcttgttg tgccactcgc attaaattct
    52141 catatttttc tggaaaatca atcggcttca cgcagtgtcc tcctcttgct catattcagc
    52201 tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt aaaaataact gaagcaccct
    52261 ctaaaagcta gattttagat ctgactttta gaaggtgctt tcaactgtta ctgcttttta
    52321 ttgctcatca gctgttgact tcctggttga acgctttgat cagattgatc gtaacaacac
    52381 ttgtattagt gaaaaaatcg cccagatgtt gctttttctg attaaagact gctggaagat
    52441 accctaacat cagcaaagga aattttagaa tgaaccggca agcaccttct ctaactaaaa
    52501 cggttgacca gctaagttct tcttcatcaa agctgatgac tcgaatacca aagatcattt
    52561 tcccaatcgt ttggccatga ttgaatttcg tcaataaaat aaaatacgct agataaataa
    52621 gtaaggctgt gaatccgtag agactcagat aagaatctga aaaattaagc caacctagat
    52681 ttttggcaat accaagtgtt aaacgcgtaa tagctccgat acacaaaaga tcgaccaaga
    52741 aggcaaacat tcggataaaa aaacctgcat agaaatactt aggaaaatca tttgcttttg
    52801 gcggttcatt tcttgtgtcc gtatactttt tccatttacg ttgtttcttt ttgatttctt
    52861 cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt ttgggaaaag ttggagtcag
    52921 gtcctacagc aggctgctgt tgcttatctt tttcttgttc cttattttca tttgaaagct
    52981 ctgacatgct tactcacctc cgtaataata cattgctttt ggtgattccg ctgtccctag
    53041 attctcaaga acggataaga tctgactagt ctcagaaggc ttcaatccct ggaattctgc
    53101 tagtttactt cctaaccatg tactagcaaa cccagatggc gtggaagaat attctactag
    53161 ttccgcatct tcaagctgtt gttcctttcg tatagctttt aaagcatctt caggaaatcc
    53221 caattcatcg actaaaccta cttcttttgc ttggacacca tcataaatcc ttccatcagc
    53281 aatttttttg acttcttctt cagatttatt ccgtccctcg ctgacgattg ctacaaaacg
    53341 actatatgcg ctgtcgatat atgcttgaag caccgcttct tcttcttttg tctctggtcg
    53401 cgttcctgaa cccatatctt tcaacgctcc gcttttaact gttgtatctt ccacacccag
    53461 tttttctaga agtcctgaat aattcaatcc tgacatgatc acaccaattg agccggtcac
    53521 tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa atatagtagc caccgctagc
    53581 tgccatgttt ttcatgctga catacattgg gatatcatgt tctttacgaa tcgtatccaa
    53641 tttcttagcg atttccgcac tttcgtagac accgccacca ggagaattta cttctaaaaa
    53701 gatacctttg actgttggat cttcttcaat cgccttcagt tgttccataa aatttttatg
    53761 attgtaacct tcagaagcaa ataaacctga agaccctccc gcagcaatcg ttccatctac
    53821 tgataatttt acgatttttt gcgtactgtc tccctcttcc aacacatttg gagctagttc
    53881 ttcatcccca tataaccaac tgttcaatcc ctcaatttga tcgttttcct gaggcttagt
    53941 caaactagct gaaactaacg aaaccacaaa taatcctgca gcaatcagaa ctgctgccca
    54001 acgtctttta ttcattcttt ctcctttcta atacaagttt tttatgacga tcacagtttc
    54061 tgactgctca gaagtgtcga accgctctgc tttttcttcc cgcgcatacc cggaaaacga
    54121 ctcttttgtt aattcatctt ctaccgtaaa ctcacgagct tgagcaactt ccaaggcatc
    54181 aggtgcgatt tcttggatgc tatcccagcc agtatctaaa aatattttcg tatcatctga
    54241 tacaccagct gatgcttcga atttggacaa ttgttttttt aacattccca gtgtcatgat
    54301 ctcaattttt gaatccattt ttactcctcc tctatccgat tactttctat tgtaccgaac
    54361 tcctgaaaaa gatgatagat aaaaacccat tattttttga cataaaaagg acatttgaca
    54421 aagaatttga tatataccaa tttatttttt attctttttt tgttgacaag aacattcgca
    54481 aaaggttatg attttaaatg tttccagata aaaaacaaaa aggaggatag ttataccttt
    54541 taggatagcg ccaggtctgt gtaaacagat gacgaggaga gggtttatcg aaagattcgg
    54601 cggatggccc tagggactgc actctacaga aaatccacaa aaaatgtctg caaagataaa
    54661 acaaaaggat tttcctgcag ctggaagcat tcatacgaat tcattataga aagtaggatg
    54721 ttttttatgt gcggaattgt tggaatgatt ggattaaaaa atgtaacacc aggattgatt
    54781 gacggtctag aaaaattaga ataccgtggt tatgattcag ctggtatctt tgtctcagat
    54841 ggcacaactg attatctggt caaagcacaa ggcagaattc aaaatttgaa aaacaaaata
    54901 aatattgata ctacaggaaa tataggtatt ggtcatacac gatgggcaac tcacgggcaa
    54961 ccttctgaag aaaacgccca tccacatact tcccaaagtg gacgcttcgt gctcgttcac
    55021 aacggcgtaa tcgaaaactt tgaagaactt aaaattgctt atttagcaaa tgaccacttt
    55081 attggtgaga ccgatactga gattatcgct catctaattg aaactttcgc gaaggatact
    55141 acaacgcaag aggcgttttt aaaagctttg cgggtcatca aaggttctta cgctttcgct
    55201 ttgattgatc ggacagcacc tgatgttatt tacgtcgcta aaaataaaag tccactatta
    55261 atcggactcg gcgatggatt caatgtgatt gccagcgatg caatggctat gcttgctcat
    55321 acgaaggaat tcgttgagat tgaagatgaa gagatggtta ccgtcacatc agaaaaagtt
    55381 attattcaaa attttgcagg tgacatcgta gaacgttctt cattcgaagc acaagtagat
    55441 gcttcagata tcgagaaagg tacctatcct ttttatatgc tgaaagaaat cgacgaacag
    55501 cctattatca tgcgtcgtat cgctcaaaaa tacgtaacag aagacaatcg tgtctctctt
    55561 gatcaaaagc tggtcgatac attgtctgat agcgatcgaa tctatatcgt tgcttgcgga
    55621 actagttacc atgcggggtt agcaggtaaa cagactttgg aaaaattgac acagattcct
    55681 gttgaggttc atttagccag tgaatttggc tataacacac ctcttctttc aaaaaaacca
    55741 ttctttattt tcttgagtca aagtggcgaa acagcagaca gccgccaagt gctggtaaaa
    55801 atcaaccgtc tcggctaccc ttcacttacg attacaaatg ttgctggttc tactctttcg
    55861 cgggaagcat cctttacact gctattgcat gctggacccg aaatcgcagt agcatcaacg
    55921 aaagcttata ctgcacaaat agctgtacta actttattag ccaaagctat cggtgataaa
    55981 aaagaatttg aaacttctct tgtattcgat atcactcatg aattgagttt agttgctaat
    56041 gcgatggaat cggtcatcgc ccaaaaagat tatttagaag agttggcaga agtatatctc
    56101 agcgatacaa gaaatgcctt ttacattgga cgtggtgctg actacaacgt ttctcttgaa
    56161 gctgctttga aattgaaaga aatcagttat atccaggcag aaggttttgc cgctggcgaa
    56221 ttgaaacatg gaacgattgc tttgatcgaa gaaggaacac cagtgatcgg aatcatcagt
    56281 gaagaagtga ctggtgcgca tactcgagga aacctgaaag aagtcgaaag ccgtggcgct
    56341 aaaacactag ttattgtctc agagggatta gaaaaggaaa gtgaccagct ggtgctacca
    56401 actgtccacc cttatctaac tactctaacc acagtcgtac cgactcaatt acttgcatat
    56461 tacgcaacgc tattacgggg attcgacgtt gacaaaccac gtaatttagc gaaatcagtg
    56521 acagtagaat aataaaagaa caaggaccag tgtcagacat ctgacactgg tccttgttct
    56581 tttatttttt atgaaaatat ctcttaatcc agtccgattt cttctttgac tactgccgcg
    56641 attttatcga catagtaatt aactttctca tcagtaggcg cttctgccat cactcgaagt
    56701 aatggttctg taccggaagg acgaactagg attcttcctt ctgatcccat ttcagcttct
    56761 gcctcttcaa tcactttttt gattgccgga acatccatcg caccgttttt atcacttacg
    56821 cggatattca ctaatttttg cggatagatt gttacctctg ctgcaagctc tgataatttc
    56881 ttacctgtct gtttcatgat attcagtaac tgaattcctg aaagcatccc atctccagtt
    56941 gtgttaaaat ctaagaaaat catgtgccca gactgctcgc cgccaaagtt ataatcgttt
    57001 ttgcgcattt cttctacaac gtaacgatca ccaacttgtg taacgacatc tttcaaaccg
    57061 attccttcca ccgctttgtg gaatcctaag ttactcatga cagtcgtaac gatcgtatct
    57121 tttttcaatc gatttttttc tgccaagtat ttagcacaga taaacatgat tttatcaccg
    57181 tcgacaatgt ttccaagttc atccaccgca ataatacgat cgccatcgcc atcgaaagct
    57241 agtccagcat ctgctccttt ttcaacgacc atctcagcta aacgttctgg atgggtagag
    57301 cctacgccgt cattgatatt taacccgtct ggatttgtgc ccattgtata aaaatctgtt
    57361 tcaagatcgg caaacaaacg attaacactt gttgaagttg ctccgtttgc tgcatcgaca
    57421 caaatggtca aaccggaaag gtcaccgttg atcgtttgta caaggaattg agaatatttc
    57481 aataagcctt ctggaaattc ttctacagtt cccaatcctt cagctgatgg acgaggcaat
    57541 tcatccactt cagcatctaa taatgcttca atttctagct cttggtcatc tactagttta
    57601 aaaccatctg caccgaaaaa tttgattcca ttatcttgtg ctggattatg agaagcagaa
    57661 atcatgaccc ctgcgcttgc tttttgcaaa cgtgtcagat aagcaacacc tggtgtagag
    57721 atcacaccta gctggaacac ttcgataccc accgataaaa gtcctgaaat cagcgcttct
    57781 tctaacattt gacctgagat tcttgtatcc cgccctacta acacgcgtgg gcgttttcct
    57841 tcttcttcat gctggcttag gacataacct ccgcaccgtc ccagcttaaa tgcaagttct
    57901 ggcgtaagtt ccttgttggc ttcaccgcgt acgccgtctg ttccaaaata tttacccata
    57961 gttgtaaaat tactccttta acttaatttt ctgaggaatt tgttctgctc atttgtgttg
    58021 attcgcttgt ttctgatgtt tcttctgtac ttgtggtcga gctgcttgtc ccagattccg
    58081 aactggaaga aatggttgtt gtcgatggtt ccgttcctga atcagcagat gaaatacttc
    58141 tactcgtact tgtattttct tgtgtccgac tgctcgtcga agcagagcta ctgcttgacg
    58201 tttgcaacac gggtgtaatc tccacactaa ctgattcggg cttgatactt aacccctctt
    58261 ctactggtat ttcaatttta cgagttgttc gatgattgat acctgagaca ttgatcggta
    58321 cagcaatact gtcaccgatc gatgcaagca aatcgctaga gccgcttact tctgcttcca
    58381 gttttgaaaa actgaaatca tagctttcta ccccggcagc tggtgttcct tgctgtatcc
    58441 cataaagtcg taccgacttg cttggcttga tcgaatcagc tctcactgtt acttgcactg
    58501 gatcagagac gatacttaat acttcccctg ctgcattcag cgcctgtatt gggacctcac
    58561 tattaatccc atcatctgtg accatcgaag cgtccacatc agctactact cgtttgattt
    58621 ctgccaatgt tttgtcacct gtagtgatag agactttttt aggactaacg gaagtatcat
    58681 caagttcata tccagatggt gtgaacgttg aaggaatcaa cgtttccaca tcgaaatctt
    58741 ttattacctt tttctcaacc gttactgtaa tagtcgatgg ttcgatcttt gctgtgacag
    58801 cactgctcaa attttgaatt tttagcggaa cttcatgtgt tccttcgccc agcttagtga
    58861 tatctgctgt gacgcggaac atccgtgtat cttcgtttgc ctctgcattc aactgaactc
    58921 ggtttgcact ggaaagtttg acagtcactg cattttcata tccatggata tagtagcgct
    58981 ttgaatcata agaaatcttg atcggcaccc ccgtaactgt ttgattatac gcttcatttc
    59041 cagaaagcgt atttggaacg gactggccat ttgcattgaa gtacagcgca atactgaaca
    59101 gcaatgcaat aaaactgtaa agaatgcttg tccgtttctc ttttgagatc atttgcgatc
    59161 ccctccctta gtcataccgt ctaggaaaga ttgaatcacg tttttcttgt tttttgtttt
    59221 ctcttcagca attagttctc tacgcaagat tgccaaatat tcttgctggg ataagccagc
    59281 catcagttca ttatctaacg tgatgctgac gtccccggtt tcttcagaga caacgatggt
    59341 aatcgcgtca ctgacttcgc ttataccgac agctgcccgg tgtcgagtcc cgaattcttt
    59401 agggatcaac atactttctg atagaggaag ataggcgctt gctactgcga tctttccttc
    59461 ttttacaata actgcaccgt catgcagcgg tgtattagga ataaagatat tgatcagcag
    59521 ctccccagtg atatctgcat ctaaagcaat ccctgtctca atatactcat ctaatccagt
    59581 atgacgttcg atagtgatca acgcaccgat ttttcgcttg gacatatatt ggatggcttt
    59641 gtcaaaagac aagatcatct tctcatcttc ctgctgttcg ctttttgatt gtttgaaaag
    59701 cgaactgcgt ccaagatgtt ccagtcctct ccggatttcc ggctgaaaaa tcacgatagc
    59761 agctatcacc ccgtacgtaa tgacttggtt catcagccaa gacaaggtgt gcagaccaat
    59821 gatttccgct acgattcgga taacaataaa aatagctaca cctttcagaa gctgaatcgc
    59881 ttttgtgcct cgcagcagtt ggatcagttt gtatacaaga taccagacaa ccagaatatc
    59941 aagtatgttc accaaaaaat tcagagagaa caagtcaccg gaaaacattt gctgccaata
    60001 gtccaaatta aaaagttccc cgatttgaaa tgacataaaa agcctctctt ctcttttgag
    60061 atttcctctc tagtatatca tatgttttaa ttttagaggc tgatatgctc gtatttttct
    60121 tgagaaaaac gcataatttt caagaaaatt gcaagtatgc gttttatttt tctctgcaat
    60181 aaatattttt aaacttcaga gaattattca ttatattttt tctgttattt ttcctttttt
    60241 tcttttgtag cttgttccat ttttttacgt cgttctgctc tggcagctct tctagcagct
    60301 cttctttctt ctgcttcaag gtccgtcgtt ttttcttttc tagtcttaac gggtacatcg
    60361 atagattcct ctggttcaag tcgtttcttg attttttcta attctcccgt cagtgcagca
    60421 tagttgtaaa aacgtgtttt agcgtcgtgg acagtctttt caaataaagc ttttgcaaga
    60481 tttggatttg cgttatctag cgaggaaaag cgaacttgtt tcctcatgaa atcaggcata
    60541 gcagagaaat caggtttttt gtagtccaaa gtcattgggt tcttgttttt ttcgaccatt
    60601 gtagggttat atcgatataa tgaccaataa ccagaagaaa cagcttcttt tgcttcttta
    60661 agcgattctt tcattccgcc aagcaagcca tgtgtgatac aaggcgtata ggcaataatc
    60721 agagaaggac caggatactt ttctgcttct tcaaatgcct tgatcgtttg catttgattg
    60781 gcacctgaag caatttgcgc cacataaaca tttccatatg tcatcgccat cattcctaga
    60841 tctttctttg aagcatactt tccactagcc gaaaatttag cgattgccga agctggtgtt
    60901 gcttttgagg tttgaccgcc agtattggaa tagacttcgt tatccaaaac aaggatattc
    60961 acgtctgctc cgctggcaag tacatgatcg atcccgccaa aaccaatgtc gtaagcccat
    61021 ccgtctccac caatcatcca ttggctgttt tttacgaata gatcctttct cttgtatata
    61081 tcctccagtt cagggaaaat tgatcgttcg ctttcgatcg cagcttgcag ttttgctgct
    61141 ctttgctgcg ttccttcact ctcaaacatg tgtactaacc agtcttccat caattctcgt
    61201 agttcttctg atacttttgg caatacttct ttgattgaac ttgccaaagc ctcgcgacga
    61261 gtctgtgccg ctagaagcat accataacca aattcagcat tatcttccaa taaagaattt
    61321 gaccatgcag gcccttgacc acattcattg gtcgtataag gagaagcagg agaagcaccg
    61381 ccccaaatag aagaacagcc tgtcgcattc gcgatcatca ttcgatcacc gaacatttgt
    61441 gtcaataatt ttacataagg tgtttcgcca cagcctgagc aagcgcccga gaattcaaga
    61501 agcggttgat tgaattgtgt agacaagaca gtattctttc ctttgactgg atttgctttt
    61561 tgtcttaatg tcattgcaaa agcccaattc acagcttgtt ctttctgttc ttcataaggc
    61621 ttcatcacta aagctttttc ttttgcggga caagcctcta cacacagacc acaacctgta
    61681 cagtcttcca ctgaaacttg gatacggtat tttacgccgt ctgcgcctcg catttctctc
    61741 acgataaatc cttctggcgc ttcttccatt tcctcttcat ctgctaaaaa tggacggatg
    61801 gcagcatgag gacaaacaaa ggcacattca ttacacatcg tacagcgatc actaatccac
    61861 tcaggtactt ctaaagcgac gccccgcttt tccatagaag ccgttcctaa aggcatttcg
    61921 ccagttgtca ttccatttgc tacaagtgta ccaacagaga gttgatttcc ttcctgtgca
    61981 ttcactggtt caagtatttc ttgtatataa ccagtgttcc ttttattgat tttttcaggc
    62041 atctccaacg ttctccaatg gtctgggatc gcgacttgat gaagcagttc ggtcgtccga
    62101 tcgattgctt gtatgttttt ttctacgata gccattgatt tatgtccata gcttttcagg
    62161 gcttcttctt ttagcagcgg aagcacctca tcaaaaggaa tgatgtcagc taatttaaaa
    62221 aaagccgttt ccattgccgt attgatccgg cgtcccaaac cgacttcttg ggccaatctc
    62281 atggcattga tcgtatagaa tctaatgtta ttttcagcca agtaacgttt taattttaag
    62341 ggcagatgcg tttctaattg ttcgtcgctc caaagagtat tcaataaaaa aatgccacct
    62401 ggcttcaatc ctttgaccaa gtcatagcta tgcaggtaag ctggtgtgtg acaggcaaca
    62461 aggtccgcat gctcgacaag atacgctgaa cgtatcggcg tatctccaaa acgaagatga
    62521 gagacagtca gaccgccaga ttttttcgaa tcataataaa aatagccttg cgcatattta
    62581 tcggtgtgat ctccaataat cttgatagct gatttatttg ctccaactgt tccgtcagat
    62641 ccaaatcccc aaaacttggc ctggaatgtt tgcggttctg tcaaatccag actttccttt
    62701 ggttccaaag attgataagt cacatcatcc acgatcccta tggtaaaccg ttttttgcgt
    62761 atgcttgggt cttttttcaa ttcatcaaaa actgctgaga tttggtcggg agtcacatct
    62821 ttagacccca atccgtagcg accaccaata acagccgggc gaagttcaga atcatataac
    62881 gcactttgaa cgtctaataa gagaggttct ccacctgccc ctggttcttt acttcgatca
    62941 agaacagcaa ttgtttttac tgtatgaggc aacttttgca cgaatgtttc cactggaaaa
    63001 ggacgataaa gatggatatt caaaaaacct gttttccgtc cgtttgcatt caagtaatca
    63061 atcgtttgtt caatcgtctg tgcaactgat cccatagaaa caatgacttc ttccgcatcg
    63121 tctgctccgt aataagtcac caagtcataa gaagttcctc gtaatcggtt gatctctttc
    63181 atatattttt gaacgatttc aggtaattta tcataatagg ggttgatcgt ttcccgttgt
    63241 tgaaaatgga tgtctggatt ctggttcgta ccactaacag tcggatgatt gggattcatc
    63301 cctcgtttac gaaatgaagc taatttctcc tgattgatca gaggagctaa ttcttcatat
    63361 tccaaaactt cgattttttg aatctcgtga ctcgtacgga atccatcaaa gaaattcaaa
    63421 aaagggactt ctccttcaat agaagcaaga tgggcaacag gtgctaaatc catcacttct
    63481 tgtacactgc tttcagctaa catagcaaat cctgtttgtc ttgcagccat cacgtctccg
    63541 tgatctccga aaatattcaa cgcatttgtc gtcactgccc gactagcaac gtggaaaaca
    63601 cttggcagta gttccccagc aatcttgtac atattcggta tcatcaaaag cagcccttgt
    63661 gaagcggtat atgtagttgt cagtgcacca gttttcaatg atccatggac cacaccagct
    63721 gctcctgctt cggattgcat ttctactact tttactggtt gaccgaatac gttttctttt
    63781 cctgctgctg accattgatc tactaattca gccatggtcg aactgggtgt tattggatat
    63841 attgcagcaa tctcggtaaa tgcataagaa atatatgcgg ctgccgtatt gccatccatt
    63901 gttttcattt ttcgcatgat tcatcatcct tgttcatatt ctctcttatt atctgttcca
    63961 ttataacgta aaacatgcca tttttttctt ttatttataa aaatatttta catgtatcta
    64021 tttgaattgg tcatctaatt tttagacaaa cagtctaaat cgttgccttt ttttgtcaga
    64081 catgtataaa ctataagcga aagaggtgtt ttaacatgga tatatcagtg attgatgcaa
    64141 cgaaaacaaa tacacaaaaa ggaatccttt atggttcatc gaatgctcct aaaaaaatgg
    64201 tcgaattcat caacttagct tgtccgtatt gccgacaatg gtttgaagaa tcttatgagc
    64261 tattagaaga agcagtccaa agcgggcaat tgcagcgagt gatcaaattg ttcgataaag
    64321 aaaaagaaag cttacagcga gggaacgtga tgcaccggta tctcacaatc agcgatggcc
    64381 aaaaagcgat caaagagatc aaacaaatat tcgatactca agacgagtgg aaacatcttt
    64441 ctctccaaga agtagcggat tttgcagtag ataaactaaa gctgacagaa caaaaagacg
    64501 aacagctgag ccaagctgtc atcaacgaag ctgagcaagc acatattcga tttgttccaa
    64561 ctgtcatctt gggtaaagaa atctttgatg aatctatttc aatagaggaa ttaaaagaat
    64621 tgatccaaac aaaataaaaa aataaggagc gtgggacaaa agtaaagaac acttttgtct
    64681 cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat ttcttggagt
    64741 taaacgcttc tgtcccagcc tccttattcc gaataaatgg tgtccaaaag cttgcatctg
    64801 ccagattatc tcatttctta tgacaagcta agcaaattat ctccaccaaa taatttattc
    64861 atatttttga cttgcgcata ctaactaatt cttaaatttt catacaaaat ttctcaattt
    64921 cccttaacga gtaatattta tgtacggtga ttctcactag tatcccaatc actttttaag
    64981 ttatcccgat ttcaattcta cttcaaaaat agttgctttt atgtatgctg ttagttgatt
    65041 agatggtcat ttgttgagct aggtcgttta ttttaaacaa gttgtttctt tagcttatac
    65101 ctatctcgtc taccaaacgc aagatggcaa acacaaatgc atacgcatct gtatttgcca
    65161 ctttgattcg tataaagcta acaactatta actcctctca gctaatagtt gctctctttt
    65221 ttattcagat tcatttttgc tgatcacagc ctagtatttc aatggttttg aacactaaac
    65281 caaattgttc cattacaaca ttcactggct gatgaaatgg attagtagat ttcgattatt
    65341 caaagcgatt ttctattggt ggatcgtgtt cccaactaat aagacggatt ccaaacctat
    65401 cgtacagatc ttctaaacca cttataaatt ttgcacctat ttcaggtgtt ttattgcgtg
    65461 gatattctat tgttacgcca ttaaatggat accaattagg attaggctct ctttttttct
    65521 gtacatctcc catagaatag ttagcacttt ctgggacata atatattctt cgaccatcct
    65581 ctaattcatc taataagata tttcgtgatc gtctatcact tgctaaatgt agacgccacg
    65641 tgcgatacca tgccatgcta cctgcatctg cagaaaatcc atcaaataat gcaacaattg
    65701 tatcttctat cacaatcctt tccccatcta cttcatattc taatctaaaa tcaaactccc
    65761 ctcgggtaat ttctggtctt ggtggcttag gacccaatat tattctactt aaaaccgcat
    65821 tcgttcccag atgaaacaca gctataacaa caataatgat taatattttg tttctatttg
    65881 ataaatttct cattattcgc acatcacctc tacattctca catattagtt cgtaatactc
    65941 ctttaatatc aaaatatcta ctggtcgtaa taatattcgc tatgaattcc tactactcgt
    66001 ataagaaagg ccaaatcatc agcttttact tgctgatgat ttggcttgtt tctatttatc
    66061 gtaattgttt tctaatcacc aaaattgtta acaatcggtt cagatatttc ccagttgatt
    66121 aaggtaattc cgtattgtgt caaaacttct tgcgcttctc cgaaattaag tacagttctt
    66181 atgtatccac tttctcctaa tataaagtct acctctcttt taaaaccttc ctcgagattt
    66241 ctaagggcta tatgtggttt taatgacatt gattgtacat tttcccccat ataatattgc
    66301 gcacttcctc gactaaaata gattcctaga tcgtccccaa aaggtagatc ttctctacca
    66361 ctagctaatc tacttctcca tcttcgttct cttttcatag aacctgcgct gaactcacac
    66421 ccgttaaact cagctatgac agtatcttca attatgttaa tctgtccatc tagttcgtat
    66481 tcaatcctaa atggaaattc tccataggtg atttccggtt cgggtatttt aggtgagatt
    66541 aatgcaaaga aaggaaatat taagcttggc aacatcaaaa aaactcctcc tatgatcaca
    66601 attcccgatg taatccaaaa tataatttgt acaattctca ttgatccatt gtattccatt
    66661 ttattccccc tattactatt catatccgcc tatctatatc gatcttccaa tgcagagata
    66721 attgcttcca taccttgatt ttttactgag ttacctatgc gagatatcat tctccactaa
    66781 ggtgcatcat attgtctatc taacatgatt aattctccaa taggtgtata ctcctccaaa
    66841 aacaacattt aatgcatctc cctcgacaac ataagctacc atgtttgctg tatatgtgtt
    66901 tgagtctaat tcataagcag ataaatttac tgtcgctgga ttaaatataa tcgcattcgt
    66961 atttatagct actgaatttg ctgcttcagc accacctttt gaatgtccga tcattgtgat
    67021 ttcaatagta ggatttacgt ggacagccct caccgtttac cctgaccatt atagaaatcg
    67081 cttacttttt tgaactacgc aacaaaggat acaatgctcc taacttcact gtctccattt
    67141 ttgtccacta aggaatataa aaaataggca ttaattcatt taattcggtc acaacacgtt
    67201 accttttaaa tcattatact gtatctcata cattattatc aataaactat tataaaaatg
    67261 caaaaaatgc tttatcaatc tattttgtaa ttggtcataa tatcattgaa atcataccct
    67321 gtatcgattt ttcctatgaa tctcgtctat ttgtgattga cgaaaaagag gcaagacaga
    67381 cactggaatg gtaattttac ccactatttt gctccaacta tttgtaatcg tacccacttt
    67441 tgaaataccg agatgatcat tttaattgat atgctgattc ccaagcaaaa ccccaagaaa
    67501 ttaaatgctc tatcgcatct agcttcttgg gttttgctta tataaattcc aacatttaaa
    67561 ttttttatgc tgttcggtct cagaaatgcc gtttttgctt atatcacaat atcaagatgt
    67621 caaattattc gtgttgccga tgactttgat taattttttt caaattgttt attaagacta
    67681 atgctgtctt tttgcagata ccaatgttcg aactttttaa cattatcata tgatcctgca
    67741 agttttaact tcttaccatt agatgttatc aaagttataa ttaaccttgc ctttttccca
    67801 ttaggatctt ttattttatg aattttctgc aggtaatctc tcctaatggc agctattttt
    67861 acctcatcag tgctgataaa ccattgatca tccacataca tactgcctgt tatgttgtgt
    67921 ttttccattt cttccgagaa cgaaaagttt aaggctcgtt cttacttcca aagttgaatg
    67981 agtccatgga ttggacttag tgtgagaatt gagaacaaca ataagaatcc atatcctccc
    68041 gcaaacatcc ctaaggctat actgtctctc catccataag taggattaga agagaaaaga
    68101 gtcaccgttt gtaataatat gaaaaggaat aatccagcac aaacaccaaa gataacaact
    68161 ccaaacattg aatttttata caatgaccat ttatttatag gcttttcttt cattttactt
    68221 ccccctactg ttctgttcac acggaatgaa ttcaaagttc ataagccctc ctctttgaac
    68281 atatgaatac gacttgctaa ctatacttta aaaaagcttg ttttaagcgg ttataggcga
    68341 gttgtaatct ctattcatct cattagcaaa taacttgccg aaaagtctac tgaaattaaa
    68401 gcagatagtg gaagaattat ttagccagga tagacactat ttgtgaaaca catgtcttta
    68461 cttttgaaat tctatgatga aaagccttgg aatacagtag ctcttaattg gtaatacttt
    68521 ttccattata aataacctct tttcatttta tatttatccc ctctaaaaat aatatctaat
    68581 ctaatatttt aaacttatat ttccctcgct gtgctaatag agcattacta aataaataga
    68641 aactagaaaa aagcattacg cctgccagaa taaaccaatt atttgt
//
LOCUS       DACDRK010000007        67219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDRK010000007 DACDRK010000000
VERSION     DACDRK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.007"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(165..464)
                     /locus_tag="IWJ77_000545"
     CDS             complement(165..464)
                     /locus_tag="IWJ77_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9452194.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            complement(609..1568)
                     /locus_tag="IWJ77_000546"
     CDS             complement(609..1568)
                     /locus_tag="IWJ77_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9452195.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            1880..2512
                     /locus_tag="IWJ77_000547"
     CDS             1880..2512
                     /locus_tag="IWJ77_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9452196.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            2635..3273
                     /locus_tag="IWJ77_000548"
     CDS             2635..3273
                     /locus_tag="IWJ77_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9452197.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(3392..3464)
                     /locus_tag="IWJ77_000549"
     tRNA            complement(3392..3464)
                     /locus_tag="IWJ77_000549"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3429..3431),aa:Thr,seq:cgt)
     gene            3585..3827
                     /locus_tag="IWJ77_000550"
     CDS             3585..3827
                     /locus_tag="IWJ77_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452198.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(3966..4595)
                     /gene="upp"
                     /locus_tag="IWJ77_000551"
     CDS             complement(3966..4595)
                     /gene="upp"
                     /locus_tag="IWJ77_000551"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9452199.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(4729..5676)
                     /locus_tag="IWJ77_000552"
     CDS             complement(4729..5676)
                     /locus_tag="IWJ77_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9452200.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(5829..7073)
                     /locus_tag="IWJ77_000553"
     CDS             complement(5829..7073)
                     /locus_tag="IWJ77_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9452201.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(7248..8273)
                     /locus_tag="IWJ77_000554"
     CDS             complement(7248..8273)
                     /locus_tag="IWJ77_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9452202.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(8295..8474)
                     /locus_tag="IWJ77_000555"
     CDS             complement(8295..8474)
                     /locus_tag="IWJ77_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452203.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(8467..9306)
                     /gene="prmC"
                     /locus_tag="IWJ77_000556"
     CDS             complement(8467..9306)
                     /gene="prmC"
                     /locus_tag="IWJ77_000556"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9452204.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(9299..10372)
                     /gene="prfA"
                     /locus_tag="IWJ77_000557"
     CDS             complement(9299..10372)
                     /gene="prfA"
                     /locus_tag="IWJ77_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9452205.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(10375..10959)
                     /locus_tag="IWJ77_000558"
     CDS             complement(10375..10959)
                     /locus_tag="IWJ77_000558"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9452206.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(11065..11534)
                     /locus_tag="IWJ77_000559"
                     /pseudo
     CDS             complement(11065..11534)
                     /locus_tag="IWJ77_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            11640..12242
                     /locus_tag="IWJ77_000560"
     CDS             11640..12242
                     /locus_tag="IWJ77_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9452207.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(12346..12915)
                     /gene="lepB"
                     /locus_tag="IWJ77_000561"
     CDS             complement(12346..12915)
                     /gene="lepB"
                     /locus_tag="IWJ77_000561"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9452208.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(13145..13912)
                     /locus_tag="IWJ77_000562"
     CDS             complement(13145..13912)
                     /locus_tag="IWJ77_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452209.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(13909..14388)
                     /locus_tag="IWJ77_000563"
     CDS             complement(13909..14388)
                     /locus_tag="IWJ77_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9452210.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(14513..15250)
                     /locus_tag="IWJ77_000564"
     CDS             complement(14513..15250)
                     /locus_tag="IWJ77_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9452211.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            15411..16811
                     /locus_tag="IWJ77_000565"
     CDS             15411..16811
                     /locus_tag="IWJ77_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9452212.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17034..18386
                     /locus_tag="IWJ77_000566"
     CDS             17034..18386
                     /locus_tag="IWJ77_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9452213.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            18379..19077
                     /locus_tag="IWJ77_000567"
     CDS             18379..19077
                     /locus_tag="IWJ77_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9452214.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(19139..20290)
                     /locus_tag="IWJ77_000568"
     CDS             complement(19139..20290)
                     /locus_tag="IWJ77_000568"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9452215.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(20303..20740)
                     /locus_tag="IWJ77_000569"
     CDS             complement(20303..20740)
                     /locus_tag="IWJ77_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9452216.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(20755..22521)
                     /locus_tag="IWJ77_000570"
     CDS             complement(20755..22521)
                     /locus_tag="IWJ77_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9452217.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            22785..23462
                     /locus_tag="IWJ77_000571"
     CDS             22785..23462
                     /locus_tag="IWJ77_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452218.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            23465..24592
                     /locus_tag="IWJ77_000572"
     CDS             23465..24592
                     /locus_tag="IWJ77_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452219.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(24792..25232)
                     /locus_tag="IWJ77_000573"
     CDS             complement(24792..25232)
                     /locus_tag="IWJ77_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9452220.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(25243..27276)
                     /locus_tag="IWJ77_000574"
     CDS             complement(25243..27276)
                     /locus_tag="IWJ77_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9452221.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(27278..28981)
                     /locus_tag="IWJ77_000575"
     CDS             complement(27278..28981)
                     /locus_tag="IWJ77_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9452222.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(29075..30025)
                     /gene="manA"
                     /locus_tag="IWJ77_000576"
     CDS             complement(29075..30025)
                     /gene="manA"
                     /locus_tag="IWJ77_000576"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9452223.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(30064..31089)
                     /locus_tag="IWJ77_000577"
     CDS             complement(30064..31089)
                     /locus_tag="IWJ77_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9452224.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(31298..32167)
                     /locus_tag="IWJ77_000578"
     CDS             complement(31298..32167)
                     /locus_tag="IWJ77_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9452225.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(32871..34763)
                     /locus_tag="IWJ77_000579"
     CDS             complement(32871..34763)
                     /locus_tag="IWJ77_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452226.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(34760..36487)
                     /locus_tag="IWJ77_000580"
     CDS             complement(34760..36487)
                     /locus_tag="IWJ77_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452227.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(36503..37153)
                     /locus_tag="IWJ77_000581"
     CDS             complement(36503..37153)
                     /locus_tag="IWJ77_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9452228.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            37357..37653
                     /locus_tag="IWJ77_000582"
     CDS             37357..37653
                     /locus_tag="IWJ77_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9452229.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            37845..38465
                     /locus_tag="IWJ77_000583"
     CDS             37845..38465
                     /locus_tag="IWJ77_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9452230.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(38557..39723)
                     /locus_tag="IWJ77_000584"
     CDS             complement(38557..39723)
                     /locus_tag="IWJ77_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9452231.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(39735..40088)
                     /locus_tag="IWJ77_000585"
     CDS             complement(39735..40088)
                     /locus_tag="IWJ77_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9452232.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            40216..41238
                     /locus_tag="IWJ77_000586"
     CDS             40216..41238
                     /locus_tag="IWJ77_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9452233.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(41677..42111)
                     /locus_tag="IWJ77_000587"
     CDS             complement(41677..42111)
                     /locus_tag="IWJ77_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452234.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(42221..44080)
                     /locus_tag="IWJ77_000588"
     CDS             complement(42221..44080)
                     /locus_tag="IWJ77_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9452235.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(44198..45358)
                     /locus_tag="IWJ77_000589"
     CDS             complement(44198..45358)
                     /locus_tag="IWJ77_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9452236.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(45665..47089)
                     /gene="araA"
                     /locus_tag="IWJ77_000590"
     CDS             complement(45665..47089)
                     /gene="araA"
                     /locus_tag="IWJ77_000590"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9452237.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(47105..47803)
                     /locus_tag="IWJ77_000591"
     CDS             complement(47105..47803)
                     /locus_tag="IWJ77_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9452238.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(47760..49421)
                     /locus_tag="IWJ77_000592"
     CDS             complement(47760..49421)
                     /locus_tag="IWJ77_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9452239.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            49639..51039
                     /locus_tag="IWJ77_000593"
     CDS             49639..51039
                     /locus_tag="IWJ77_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9452240.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(51291..52370)
                     /locus_tag="IWJ77_000594"
     CDS             complement(51291..52370)
                     /locus_tag="IWJ77_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9452241.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(52456..53415)
                     /locus_tag="IWJ77_000595"
     CDS             complement(52456..53415)
                     /locus_tag="IWJ77_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9452242.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(53427..54950)
                     /locus_tag="IWJ77_000596"
     CDS             complement(53427..54950)
                     /locus_tag="IWJ77_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9452243.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(54960..55523)
                     /locus_tag="IWJ77_000597"
     CDS             complement(54960..55523)
                     /locus_tag="IWJ77_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9452244.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(55529..56392)
                     /locus_tag="IWJ77_000598"
     CDS             complement(55529..56392)
                     /locus_tag="IWJ77_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9452245.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(56406..57302)
                     /locus_tag="IWJ77_000599"
     CDS             complement(56406..57302)
                     /locus_tag="IWJ77_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9452246.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(57477..58748)
                     /locus_tag="IWJ77_000600"
     CDS             complement(57477..58748)
                     /locus_tag="IWJ77_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9452247.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(58738..60255)
                     /locus_tag="IWJ77_000601"
     CDS             complement(58738..60255)
                     /locus_tag="IWJ77_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9452248.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            60453..61343
                     /locus_tag="IWJ77_000602"
     CDS             60453..61343
                     /locus_tag="IWJ77_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9452249.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(61416..62240)
                     /gene="nadE"
                     /locus_tag="IWJ77_000603"
     CDS             complement(61416..62240)
                     /gene="nadE"
                     /locus_tag="IWJ77_000603"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9452250.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(62252..63724)
                     /locus_tag="IWJ77_000604"
     CDS             complement(62252..63724)
                     /locus_tag="IWJ77_000604"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9452251.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(64361..64723)
                     /locus_tag="IWJ77_000605"
     CDS             complement(64361..64723)
                     /locus_tag="IWJ77_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9452252.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            64946..65698
                     /locus_tag="IWJ77_000606"
     CDS             64946..65698
                     /locus_tag="IWJ77_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9452253.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(65838..66761)
                     /locus_tag="IWJ77_000607"
     CDS             complement(65838..66761)
                     /locus_tag="IWJ77_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9452254.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(66776..67048)
                     /locus_tag="IWJ77_000608"
     CDS             complement(66776..67048)
                     /locus_tag="IWJ77_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452255.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        19465 a        13739 c        11666 g        22349 t
ORIGIN      
        1 atactagctg tttttgtgtt aattaaagga taaaatacaa tataaagaaa catctgtttc
       61 tttatttaga ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg
      121 agattgacta tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa
      181 gtaatcggtg ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat
      241 atctccatat aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt
      301 aaactacgtt ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc
      361 tttgcgtcta tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca
      421 tataatttta gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa
      481 aaatatattt cttcccacta cattctataa acaaagtata cgctacatct agattttttt
      541 aaatcattac tggattgtta aaacctttca atacactgtt atggcaacga tttttatttt
      601 cttaattgtc aaattaagtt agacaaatca tcaatactta cgtaactcaa aaatacttcc
      661 aaaggtgttc gataattcag tgattttcta ggaatattat ttcgtttcga tgcgacagat
      721 tgaataaaag attcatctac tgaattgaaa tccatagatt tcaataaacc atctctacgt
      781 aacaatccgt tagaattctc gtttaggcct ctttgagacg gcgttcctgg atcagcgaaa
      841 taaatggcaa tatcattggc attactgatc tgtttccaat ttgaaaattc ctttccacaa
      901 tcaaaagtga tggatttgaa tagatttttc ggtacagatt caaaccaatg atttaatttg
      961 ttttcaatat caatcgcttg tctaccacac ggtttcaatg tgatgataac ttttgataat
     1021 cgctcaacta aggtaattac agcactttta tgtttcaaac ctacgatagt gtcaccttca
     1081 aggtgaccaa actcatttga gaattggcta taatcttttt cacgttcatg aatagagcga
     1141 cggaaagctt gttttccacg tctttcttga tgtccatttg gtttacgctt gcctttcatc
     1201 ggtaagtcag aaggattaaa tagccccttt ttgaacatac gataaatggt acgagcagaa
     1261 caacgaatag gaaacgctgc acgaccaaca atcacatcgg gtgtccatcc ttgaacaacc
     1321 tttctttgaa tatattctga ttgttcctca ggtaaaacaa gcgggcgtct accacagttt
     1381 gatttgtttt tcttatactg ttgatagtac tctaaggctg attttccttg cttgaagaac
     1441 aggtatactt tatggatcgt ttgtcttgaa cgattcaaga aatgcgcagt tttcgcaaca
     1501 gattgattta ttttgaaata agactctatc attacaagtt catccgttgt aagatgggta
     1561 taggtcattt gtactcactc cttataattc tttggacgga actattttga gtgtagcaca
     1621 aatgactttt ttagttgtct agcttaattt tacaatcggc gttttaaaaa cattctaaat
     1681 taaatcagta aaaattttta ttctaagatt tttattagaa ttacgcctaa tttgtgtctc
     1741 tcagggcaat tctaagaacg tttcaagaga tagcgacact atcgttatcg aaaaaggcga
     1801 aaacctatct cattttctgc taatgtaagt tggtttagct tttgttaata tgactttatg
     1861 aaaatggagg taattaaata tgatttttcc taaagttaat gaagcaagct tgaataaagg
     1921 atcctttttt catatctatg aaaacgaaga ttttgaactg tataatatcc tttcacaaaa
     1981 agaggtcatc gaaaaccaaa taatctgcca tccaattatt tacaaatcta ttttcaagta
     2041 tgcatttttt gcttccaatg attttcaaag aatggagatt accgactgta tattcgaaaa
     2101 ctgtgatttt tcaggctgta tattgacaaa aagcatgttg catcgcgtga aatttgtcaa
     2161 ttgtaaatta acaggtacta aattgatgga aagttatatt gggaacacgt tattcgagaa
     2221 ttgtaaaatg gactatgtaa atttatctgg ttcaaacata aaagaaagta cctttgagca
     2281 ctctgtctta tcaagcgcag attttgtaga ctgttccctc actaaaataa tgttttctac
     2341 taatgattta gaaaatgcta gcttttttga tactgattta aaaaatatcg atttaagtag
     2401 caactccttt gaaatgattg aagtacgctc ggaacatcta aaaaattgta cgcttaatca
     2461 gttgcaagca cttggttttg ctagaaaatt tttacaaata aaagtagtat aatcaaaatg
     2521 aatatataag ataaattttt agcaatacaa atatccataa aaaaacattg cctattttaa
     2581 ctcttcatgt atactgactc tatcttcaaa aactgaatag taggagttat tatcatgaat
     2641 aacattttag gaaaaaaaat agaacttaaa aaagggatat tgggcactat tgtcgttgta
     2701 ctcggtttgt tagggttgct atttgcgcca tccattatta gcattttttc tttaactatc
     2761 actggattta tagcatttac acttgttttt ggtaaaaaag atacgaaaaa aatgttctcg
     2821 aaacctgtcg ctcctgtcaa aaatatagcc aaatattttt ttatcaatgt agtaatcagt
     2881 gccgcagtct cggtattttt gcaacaaatt ttgaaatggg gtttaacagg caatccaata
     2941 aatgaggctt tcagcccgtt attatttatt attttgccta tcatgatact tggagaagaa
     3001 ttgttctcag tgtattttct cgctattttt tctagtaagt tcagcattcc tgtcgctagt
     3061 ttcttgtcag caatcatctt tggatttatc cattacagta cttatgataa tggaaatatt
     3121 ttacacactg tagcacacat ccttttgata caaggggtgg caagattgtt attcaaccaa
     3181 gctgccatta aaagcaactc gatcattaca agttgggcag ttcatgtgat atttgatttc
     3241 acagctatat tgctagtcgt tttattctcc taaattttta tagaaataat acatgctcta
     3301 ttgctaagaa agcatacttt atttccttta aacaaaaaga cagatgtatc aatggattaa
     3361 aaatccttga tatatctgtc ttctctttta atgccagcta tagggatcga acctacgacc
     3421 tacgcgttac gagtgcgttg ctctaccaac tgagctaagc tggccttatc caaaagaaat
     3481 tatatactct atttggctga ttgtaaagag tccgatggaa aattagttta atttcatgat
     3541 atttttcata aaacgtggta taattgatgt gaaaggcagg ttttatgaaa atggcaactt
     3601 ttggaaaatt tgatacaact atcgtaccat cggaaattgg tgaaaaattc aagatcaata
     3661 ctcacgtaat gttcacaatt cacggcaagt cgcaaactgg aacgatttca aagcaattga
     3721 aaaattccgc tgttgttgag attgatgaaa ctgcagaaaa tgcgaacttg atgacgcaaa
     3781 gtaagggtgt agtaatcgtt aactacaaag aactaaaaaa gatttgaatt tgaccatctc
     3841 tgcatttttg ggatggtttt tttatatgca aacgaaagct gtgaaaaagc tgttctgcat
     3901 caaacgttaa aaatgaccaa aaaaggatgt gacaaaagtt gtgtcacacc ctttttcgta
     3961 tttacttatt ttgtaccaaa caaacggtca cccgcatccc ctagtccagg aacgatataa
     4021 ccatgttcat tcaatttttc atctagagaa gctgtataga tgtcaatatc aggatgcgct
     4081 tcttgcaaag cttttactcc ctcaggtgct gctaccaaac aaacaaattt aatgttcgac
     4141 gcgccacgtt ctttcaatgc atcgattgcc atgattgctg atccgcctgt tgccaacatt
     4201 ggatctacta caaacaactg acgtgcatca atgtcttctg gaagtttgac gaaatattca
     4261 tgtggttcca atgtttcttc atctcgataa agaccgatat gtcccacttt agcagctgga
     4321 atcaattcca agataccgtc aaccattcca atacctgcgc gcaagattgg aataatcgct
     4381 acttttttac cagacaacgt tttttgtgtt gtttcagtga tcggtgtttc gatcacgatg
     4441 tcttccaatg gcatatcccg tgatacttca tatgccatca acatagcgat ttcatcgact
     4501 acttcacgaa aaacctttgt tccgcagttt ttgtcccgaa taatagttag tttgtgttga
     4561 atcaatggat gatcgatcac ttgaaatttg cccatttttt aagctctcct ttgttctgtc
     4621 gtttcttttt aattgtaaaa caaaatcaag tgattgacta gtcaaactcg ccatttttcc
     4681 taagaaaatc cgtttttgaa aagaagtttt cctgcggaca agcaaagctt attctttttt
     4741 cttgatccaa gaagtagata atgccattgc tgcacgcgtt gcctttgtct gatccatagc
     4801 atttaccatg acaaaatcat ggatgattcc ttggaagcgt acttgtgtaa caggaacccc
     4861 tgcatcacga agtttgcgcg cataagcctc accttcatct cttaatacat ccgcttctcc
     4921 cgttaaaatc attgctgaag gaagatcatt tagttcattt atatttgctc tcaaaggcga
     4981 agctgtgatt tcagcacgct cttcttcatt cgtcgtatat tggtcccaaa accacatcat
     5041 tccttctctt gtcaaaaaat agttttctcc gaattcatga taggaatctg tatcaaaatc
     5101 agcgtttgtg actggataat acagcaattg cttggatatt gggaggccct ttcgttcttt
     5161 tgttaagatc gtcatgactg tagccatgtt accacctact gaatctcctg caacgattag
     5221 attctcgaga tccagatttt ttgacttggc taatttattc agctgttgca acacagcata
     5281 attttgttca attgctgttg gatattttgc ttctggtgat cgactatatt ctggaaagac
     5341 aacgacggaa ttcgtacgaa ccgcaagttc tctgatcaat ttatcatggg tttgagcact
     5401 gccaaaaacc cagcctgctc catgaatata aaaaataact ggtaatttat ccatattccc
     5461 ttctggtcgt acgagacgaa tattgagttt tccccattcg ccagtatcca ccatcaagtc
     5521 ttcaatatcg acggaatatt tgttcacagg tgattcttgt acttcttcta gtaaaactct
     5581 tccttcttcc ggtgctagtt catagatacg cggatgaggc gcatttgcat cactgaatgc
     5641 tactgcttcc ttttctaacg agatacgatc agtcatgact tttcctccct gagatttagt
     5701 gtgaagacta aaattacacc tcatatacta gagagcatca ccgatattgt caaagaatcc
     5761 gttttcccaa aaaataaaca gaaaagccca acattgaact tttctgttta ctaatggaat
     5821 ttaaattttt attttttatg caacggaaat ttttcagtta attcgcgtac acctgttttg
     5881 acttcgtcta gttgagcgtt atcatctttt gcttgtaatg ctttgacaac tagttcagcc
     5941 acttttacag catcttcttc tttgaacccg cgagtagtga ttgctggcgt gccgattcga
     6001 ataccgcttg ttttgaatgg acttagtgtt tcaaaaggaa tcgagttttt atttacagtg
     6061 atattcacac tgtccaaaat gctctctgct tcttttccat ttatacctaa ctcgcgaaca
     6121 tcgatcaaca tcagatgatt atctgtcgcc cctgaaatga ctctggttcc aattgcttga
     6181 ttgaaaactt tgaccattgc ttttgcattg gcgatgattt gttcactata ttctttaaat
     6241 gcaggatcta atgcttcttt gaatgctact gctttacccg caatcacatg ctctagaggg
     6301 ccgccttgaa taccaggaaa taccgcacta tttatttttt ttgctaatgc ttcatcgttt
     6361 gtcaaaatca tgccgcctcg agggccacgc aatgttttat gagtggtagt cgttgtgata
     6421 tctgcataag gaactggatt aggatgaagt cctgctgcta ctaatcctgc gatatgtgcc
     6481 atatctacca tcagttttgc gcctacttca tctgcgattt ctctgaattt cgcaaagtcg
     6541 atcgttcgtc cgtaagcact ggcacctgct acgatcaatt ttggttgatg ttttctagct
     6601 aaaatacgta ccacattata atcaataact tctgttgtcg gatcaacacc gtaagcaaca
     6661 aagtgatagg ttttgccact gaagttgact ggtgatccat gtgttagatg tccgcctgcg
     6721 gaaagatcca ttcctaagat cgtgtcccct ggttctacca aagcgagata agcagccgtg
     6781 ttggcttgag agccggagtg aggctggacg ttcgcaaatt tcgctccgaa taattctttt
     6841 gcacggtcaa tcgctaaatt ttcaacgata tcgacaaatt cacacccacc ataatagcga
     6901 tgccctggat aaccttctgc gtatttattt gtcaagatac ttccttgtgc agccatcact
     6961 gcttcggaga caaagttttc agaggcaatc agctccaaat tatgttcttg tctttcttct
     7021 tctttggcga tcgcagccca aagatcaggg tcaaacgttt tgtaatctac cacaaacaac
     7081 agctcctttt tattttccta caattttatt tttcccatca aaaacaaatt gccaaatatt
     7141 cgtatctctt ctattacgat tagttacgct tagtttttcg ttcactgata gaagatttac
     7201 ttgatagcat tgtatcatac taaaagaatg cgacaataac caattgatca tttttcaaaa
     7261 taattttgtc ctgctgcttt tttcaagcga ttcatgtaag cattccccaa tccagtttct
     7321 ggatacactt gtacaaaaat caaatctaat ccaagtgttg gttcatctaa tcctcttaga
     7381 cctgcaaaca atcctttagc cgccgcttct actgagttgt cgttatacat ataaacagct
     7441 gcagtatctg tccgcacttg atccgcaatt tcggggcttg ctatgacacc tgcacggatc
     7501 ttcttatttt ttgcccattg aactgcagtt gaccaatcgc catctctgac catcagaaca
     7561 cgtgtatcgg gtgagtagtg tttgtatttc atcccagggg cttttggtgt ttcattttct
     7621 tttacaagat gctgatcaat agcaacagga gattcgatga ctgcttcgat ttgttctttc
     7681 gttactgcac ctggtcgtaa aatcataggc atggctgttg gatcacttaa atcaagaaca
     7741 gtggattcta caccaattcg cgtagctcca tcatcaataa tcccggtaat ttttccttgt
     7801 agatcatgat atacatgatc tgcagtcgtt ggacttggtt ttcccgacgt atttgcactt
     7861 ggaccgacaa gcggaacacc ggaaagttcg attacttcta gtgttttttt gttatccggt
     7921 atacgaaatg cagctgtgga taatcctccc gtcacaactg agggcaaagt gtcttttttg
     7981 atcttaaata tcaaagttaa tggtccaggc cagaagttct tcacaatctt ctctgctgcc
     8041 ggatgaaagt tgtccacata ctcttttact tgttcaaaag aggcgacatg gacgattaat
     8101 ggattatctt gcggtctccc ttttacagaa aacactttct tgacagcgtt tggtaaaaga
     8161 gcgttggcgc ccaatccgta tactgtttcg gtaggaaaag caacaagttc tccttctttc
     8221 agtgcttcgg ctgcctcttg caattctgtt tcatcatatc gtttcgtttc cacagtttat
     8281 ccacaacccc tttttcatac attcttattt tgtctgagtt tatcctcaag ttttccactg
     8341 tttgtggata atctatttgt caatagtgga taaacttgtt gataaaccaa aaagtcgttt
     8401 acatccttgt caaataagtg tttttttata ataaaaaaac aaataaaaaa tttttcatcc
     8461 acaacttcaa ccataaactt ggatcattcg ttcattgcca aaaagatcct tttttatcgc
     8521 cactttttta tctggaaatg cttgctgaaa tatttttttt actgcttcac cttggcgaaa
     8581 tccgatttct aaaaaaatct ttccatttga ttttagtacg cttggtgctt caactgctat
     8641 tttttcataa atagctaatc catcgttctc agcaaaaagg gccattttag gttcgaatga
     8701 ccggacactt tcatccatca gttcccattc attccgactg atgtacggcg gattagaaat
     8761 aatcacatca aattgctgat ccattactgg tcctaatgta tctccatgat aaaaactgat
     8821 tttcgttcct tctttctgag cattttcttt cgctacttct aatgcttctt ctgaaagatc
     8881 cacagcagat atatgccaat tttttcttgc tgctttcaaa ctaatagcaa ttgctccagt
     8941 ccctgtccca atatctatca cttctaacgg tacaccaggc gtctcgtcta aacaccattc
     9001 gactaactcc tctgtttctg gtcgagggat cagtgttgct tcagttacct ttaaccgatg
     9061 atcataaaaa tctgcatatc ctagcaaata ctgaggtgga tgatctgcaa gcagaagtcg
     9121 cagatcggtt tcgatcaacc gctgttcttc ttctgaaatc tcttcgttca tatgaaggag
     9181 ccaatcaagt tttttccatt gttttcgttc caaaaataaa aattggatac tatatgcttc
     9241 tttcccatga tcctctaaaa aagaagaagc ccgtgacagg acttctcgat aggtcatttt
     9301 acccattttg catagcttcc agttttgatg tttgatcata catgatcaat gcatcaacga
     9361 tctcatctaa tttccctgct aaaatctgat ctaatttttg gatagttaat ccgatacgat
     9421 gatcagtcac acgattttgc gggaaattat acgtccggat ccgttctgaa cgatccccag
     9481 ttccgactgc tgattttcga ttggcatcat actcgctttg tgcttcttgc tggatcttat
     9541 cgtacacacg agcacgcaag attttcattg ctttttcacg gttcttgatt tgagaacgtt
     9601 catcttgcat agcaacaacg atccctgttg gtaagtgagt caaacgtaca gcagatgctg
     9661 ttttattgac gtgctgtccg ccagcacctg aagcatgata gatatctgta cggatatctt
     9721 tgtcttcaat atcgatctct acttcttcag cttctggcat cacgacaact gttgcggtgg
     9781 aagtatggat acgcccttgc gattcagttg aaggaacacg ctggacgcgg tgcgcaccac
     9841 tttcatattt taatttcgaa tatacgttgt ctccggtgat catcatgatc acttctttat
     9901 atccaccgat tccggtaacg ttagcttcca tcacttctgt tttccagcct tgtgcttcag
     9961 catacttttg gtacatattg aaaagatcgc cagcgaataa tgctgcttcg tctccgcctg
    10021 ctgctccacg gatttccatg atgatatttt tatcgtcatt tggatctttt ggtaaaagca
    10081 agatcttgat tttttcttct aagacttctt tttctttttt taattcagat aattcttctt
    10141 ttgctagttc agccatttca tcatccagtt tttcaccaag cagttcttct gtatcggtga
    10201 ttccttgtac tacttcttta taacgacgat aaacttcgac tgtttcgcgc gtgtttgctt
    10261 cttcttttga aagctgcata aaacgctttg tatcagaaat cacttctggg tcacttaaaa
    10321 gttctccaag ctcttcataa cggtcttcaa ttgcttgtaa ttgatcgtac atgcttattc
    10381 tccttctgta atcattgatg ggtgaaagta atgttttcga caaacggggt aataagcttc
    10441 atttccgcca atttgcactt gatcgccttc ataaacaggg tttccatcaa tataatgtaa
    10501 attcatgatt gcttttttgt ggcagaacca acagattgtt ttcatttctt cgattttgtc
    10561 tgcataaagc aataaatatt ttgatccttc aaataactcg tttcgaaaat catttttcaa
    10621 accaaatgcc ataaccggaa tatccaattc gtccacaatt ctcgtaaaag caatgacatg
    10681 tttctttttt aagaattgcg cttcatcgat cagcacacaa tagggtttgc tttccatttt
    10741 ctgaattacg tcaaatacgt ctgtttcttc aaatatcggc atcgcattac gtttcagtcc
    10801 gattcggctt gaaacaacgc ctaaaccatc tcttgtatca ataccgcttg tcattaagac
    10861 aactggctta ttttgttctt cataattatg tgcgactttt aggatctcga tcgtcttgcc
    10921 gctattcatt gcgccatact taaaaaatag ttgtgccatg ctctttttca ccttccagtt
    10981 tgttcctatg gtattatact tgattttcag cattttttac agtgagaatt ttcatcaagc
    11041 acagattagt attaaatcta ttgctcactt ttgttccaat tttttgacaa gaactaaaca
    11101 tgggttccat tcatcccata aatttggaaa aacttccaac cttgaaaatc ctaatgattc
    11161 gtagaaacaa atagtttgat catatataga gtagtgcccc tcgtctacag tcttcacttg
    11221 caaatatttg taatgttttg ctgcgtaacc ttctgcttct ttaaaaagtt ttgttcctaa
    11281 tccttggtga tgatagtctt tttttattcc catacaatag aatagatttc ggcagtatct
    11341 tcacttgtct gtgaaagagt gataaagcct aaatattctt cttttctttt ttcacaccat
    11401 aaaggtagtt tacttgattc ctcgatatat ttttccgttg attcaggaag cccaaaccac
    11461 tcaggcaagt ctagtaatat ttctcggaca cactttcctt tttcctctct atcttttaca
    11521 aacgatatcg gcatattctt tctagattct ccctttttga attgagtata gcatgctttc
    11581 tttcttttca cgtgctataa taagttaaaa tttcaaagtt ttacgtaagg agctatttga
    11641 tgacaaaaaa tatttttgat tccatcgcta accattatga ttcccctgac cgtcaagcat
    11701 tagccaagat tattcgcgaa gaactaacta cttatctacc gcgcgacagc caccaaaaag
    11761 tcctgctgga ttacggaggc ggaactgggc ttgttagttt accgcttgcg gaaagattca
    11821 aagaactgat cattgccgat gcttcagaaa ctatgctgaa aatggcagaa gaaaagatcc
    11881 aagcagcaga tctgaaaaat gtccgaacga tccacgctga tgcttctgtc gaatttcctg
    11941 ctgtccaagc taatctcatc cttctatcac ttgtactttt acacattcct gatacggaga
    12001 atattcttac aaaactttat gaaattttag ctcctggcgg tcaattgatc atcgtagact
    12061 ttgacaagaa tgaacaaatt agtcatccga aagtccataa tggctttagc caagaagaat
    12121 tgaataatcg attggaaaaa acgggctttg tctctgctac tagtcatacc ttccatcgtg
    12181 gggaaaagct atttatgaac aaaaacgctt cgcttttctt atccatcagc caaaaagctt
    12241 aaaatgagat tgcaaaaaaa gtgagcaact ccaagaaata agaaggaatt ttcggaaaaa
    12301 gaagctgtga caaaaaacat gtcacagctt ctttttattt tttcatcaca tgattttaat
    12361 ttctgaaaga ggccaataac ggaaaacaac ttttccttgg atcatatctt tgtcgactaa
    12421 cccaaacatt cggctgtctt ttgaaatcaa gcggttatcg cctagaacaa gatactttcc
    12481 ttttggcaca gtatattcaa agtcactcgt aaaggtggat gaactttctg cttgtgcttg
    12541 gaatcctgag ctataagcat attctccttg taacttatct tctgaaaatt cttttttgaa
    12601 ctcatccaga taaggttcat catacttttt gccattaatg gtcaactggt cattttccat
    12661 cttgactgta tctccaggca taccgatgat ccgtttaaca atcatccgtt cttcatcccc
    12721 cggttccttt gtagtaataa tatccattcg ttcatagttt gagatcttag atgaaatcaa
    12781 tcgttgtcca tcacttaatg tagggtccat cgaatgcccg tttacagtaa ctggtgtaaa
    12841 cacgtacact ctagctaata aaatagccgc taatacaagg atcaaaatcc cccaattttt
    12901 caaaaattct ttcacagctt acctcctact tgtattttcc tcgttattta ttacgttttt
    12961 tcacaatact tcgttgtact ttctttattc tacaatgttt ataaatgaaa ggaaagaata
    13021 agtgatgtgt tcgtcttatc tttctcgtta tataaaaaag gaacgtgaca aaagacgtgt
    13081 cacatccccc ttattccgaa taaacgtttt tatcgcaacc actgatacac ggcgtttcat
    13141 cacattatga atgtcttctt ctttggctac gactataaac cactggttct tcctgcgact
    13201 gtgccttttt tgcaatcaat ttcagacgat aaggctcatg atagcgtaac ccgtctttga
    13261 caaaatcacc gccgacgatg aacaatccga caggttccaa tgtgtcttct gttgagtcaa
    13321 gtcgaatggt ccctactttt tccatgtccg agtagcttcg tccaatattt agaacatact
    13381 ctttctctcc tgttttcttg acatagtaat aaggatacaa tgtatttcta tcgaccagaa
    13441 atccatccat gcgaccgagt tcatttgttt tgatctcacc atttccaatc aaataccgca
    13501 tattctcggc taatttcaat tgataatctt cgtcataaga aaccatgacc tcttgtgtat
    13561 tcactttatc gatcgaattt ccattatcta tcgattcttc tgtctggatg ttgaacgctt
    13621 catcaggtaa atacttcaca aaaagctgag tgacatcggc tttcgtgttt ttatctaaaa
    13681 agtaataaaa tacatttaaa acagtaaaat aaacaagcac tagaaatgca agtccatata
    13741 agaaaaattg aagatagttt tggttttgcc aaagtccata aacaattctc aataaataaa
    13801 gcgtgggaat cacacttaaa atcgtatatc cacggttaag atatttagga ctgatgtcta
    13861 aatagccaag gaatttatgc accccgttga taaggtctag taaaaatgtc attgagaatc
    13921 acctgcttgc tggttatacg tttgggtatt tcctgaatct tggttttgct gtgctgtacg
    13981 tgctgcatca tcattttgcg tgttatcatc tacagtcgta ctgcttggta tcgtttcgga
    14041 gctagcactt gtatccggac ttgtacttgg attggttgaa tccgatggag tgccatctgg
    14101 taccgtatta cttgtttcgc ttgagctgtc agttgtatat gtctcatcat acgacgaact
    14161 agttgttgta tcgtcccatg tacttgtcgt agtggtttca ttcaaacttt ccgttgttgt
    14221 tggttcagat gtcgagactg aagtagttgg tgttgtttct tctacagtac tagaacttga
    14281 acttggtgca cggtagctgg aactattcac aatacctgta gtagtcgcga caatcgtcga
    14341 gattgttaat acagcgattg gcttcaaaat aggtgctact tttcgcatct tatctcttcc
    14401 ttcattgaaa tttattcttg tttccttact ttactacgga caaattatac agtctacctt
    14461 atttctacca taaattcttt atttttttct tacgtttttc cgaaagattt ctttaatttt
    14521 cctcaaaaac tacggttgat gtttttaagt aagcttttgc ttcccgattg atttctttca
    14581 gatcgcatcc ttgctgcttg gcagcgaaca cgcaaccaca ataacattgg cgataaatat
    14641 catactcttt gcacatttgc aaagagcgtt catagccttt gtttttcttg aaatcacttg
    14701 gcaagtattg tgtcccgtaa atcttttgga tatccattcc gatctgattg atcaaagctg
    14761 catttttctt tggcgagatg gtcagggcac ttccaaagta atcgtatcct agttcttgcg
    14821 ctttttgcgc aacaagatct aatcgcatat tgaagcatgc actgcagcga gcaccacctt
    14881 ctttttcgtt ttcaagatgg tttgttaaaa ccattttggt gaattcattt ggacggtatt
    14941 catcaacgat tagcgcaaca tgatggtcag ttttctcatt aaattggtct acaaatcgtt
    15001 tttgttcatg taatcttcgc aaatactcac ttttaggatg aatattcgaa tttgaaaaaa
    15061 ataacgtgat ctctgcatat tgagacatga attccaatgt gtgcgtactg caaggagcac
    15121 agcatgtatg tattaatatc ttaggcttta cttcttcttt ctcccattgc atgatcattt
    15181 ttttcagaac tgtatcataa tttatttttt gatttttcat tttttctacg atttgttgag
    15241 cattaatcaa tttgttattc ctcctccttc ttttttctag gattttcctt attataagcc
    15301 atttctattt taatcgaaag ctgtattgtt atctgtttga taatttcttt gagtattcct
    15361 aattcccctt gtatactggt agaaacgact attgtcagga ggatagtact gtgcatgttg
    15421 atttattctt tcgaaggctg cagcggcgag gacagaagtt tgctgccaat catttctcat
    15481 cgatccaaat catcgttttt tattatatat tgatgacggt cttatcactc gttctatttt
    15541 atatgccgat ttttcgcgaa ccagatagcc atgtttcttt tgtggacatg ctgttcatgg
    15601 caatcagtac agtgagtgtg actggactat cgacttttga tatcaattct gtgttcaatg
    15661 ataacggtat tatactgctg gaaatattat ttcaagttgg tggtttgggg atcatgatga
    15721 tttctactgc ttttgtcatt ttttctaaac gacggatcac actgaaacag cgacagctga
    15781 tcatgacgga tatgaatcaa ccgcgtttgt ctggtatcgt ccgactgatt cggatcactt
    15841 ttgctatttt aatctggttt caattacttt ttgggacatt cttttctatt tatttttatt
    15901 acagaggcta ttttgatcgt tggcgtgatg ccgtttttta cggtttttat caggcgatat
    15961 cggctgtcac gaactctggg tttgacgtca ctggggattc catcaagccc tttgcacatg
    16021 actatttttt cttgatcctg atcatgtttt tgatttttgt cggaggaatt ggttttcctg
    16081 tattgatgga gtgccgggaa tggctattat acaaacgttc caatgcgaag cttccgtttc
    16141 gtttttcctt gtttaccaaa ttagctgtgt tagcttttgt tatcttgttt gtttcgggaa
    16201 ctgtcttgat ttatctattg gaaaaggacc atttattcca agattccaat atgagtgtca
    16261 aatggatcaa ttcgatgttt tattcgatta cgactcgtaa tgctggtctg cagattcacg
    16321 atttgggtga ttttcaaatc acaacactga tcattttttc tttactgatg tttatcggat
    16381 gcagtcctag ttctgtaggc ggcgggattc gaacaactac tgtagcaatc atcggtttgt
    16441 atctatattc cttcctcaaa agtgaagata acatcaatat ttttggacgg agaatcgatc
    16501 aagatgatgt gcgaaaatca gtcgttgtct ttatgctttc tcttggtatg tgtttctttt
    16561 gtatcgtttt tttatctgct acagaagaac aaacgctgat ctcgatcatc atcgaagtaa
    16621 cttctgcgtt tggaacaact ggtttatctt tagggattac cggagacttg tctgttgtgg
    16681 gcaaaatcac gattgctaca ctgatgttta ttgggcggat cggaatgcta tatacattga
    16741 tgttattcgt gccaaaagaa acacgagatc taggctatga atatccaagc gagaaaatta
    16801 ttatcggtta aaatgggaat ataggtaaga gggaataata aaatcacagc agcttgaaac
    16861 aatcattttg tttcaagctg tttttttgtt aaatcatgcg attttgtccg atttcatgca
    16921 ctttcagccg taaccttttc ttaagaataa gaaaagcaat ttaacctttt tttcaacata
    16981 tggtatgatt agttgaattt attcgtcagc aaatcgtttt tggaggttac ataatgggaa
    17041 tacgcagtca ttttgcgatt gcagttggaa aaacgtcgca atgggcatta aaaacttttt
    17101 ttaaaggcgg atcaagttta ccaggtaaac tagcgctgaa agtcgatcca catatactag
    17161 atagtttggc aaaagactac caagtcgtcg ttgtcaccgg aacaaacggt aaaacattga
    17221 ctaccgcttt aacagtcaat attttgcgcc agcaattcga tgaagttcta accaacccta
    17281 ctggtgcgaa tatggtacag ggaattgtat cgactttctt gcaggcaaaa ccaaaaaaag
    17341 gacagaaaaa gtttgctgtc ctagaaatag atgaagcaag tttgagcaaa gtcacagaat
    17401 atatgaaacc agaactgttc ttatttacaa atattttccg tgatcaaatg gatcggtatg
    17461 gagaaatcta tacgacctat aaaatgattg tagacggtgc cgcaaagtca ccaaatgcaa
    17521 cgattatcag taacggtgat tcaccaatct tcaattctat cgaaacagtc aatcctagaa
    17581 aatattatgg ctttgaccat gaagaagatc gtgaacaaat ggctcattac aatacagacg
    17641 gtgtcttatg tccaaattgt catcacatct tgcattataa aatgatcact tatgcaaact
    17701 tagggaaata ttactgtcca aattgtgact tcaaacgtcc tgaattggat tatcgattaa
    17761 ctgatatggt acggatggac aatgtgtcag cagatttcgt gatcgatggt catgaatacg
    17821 gaatcgaagt tggcggtatg tataatgtct acaatgcttt agcagctact gcagtggcag
    17881 agtacttcaa tgtcgatcct gataaaatca aacaaggttt aggatatgac gagaaagtat
    17941 tcggccgaca agaagtcatc aatatcaatg ggaaaaaatg tacattagta cttgtcaaaa
    18001 atcctgtagg tatcaatcaa gtaatcgata tgattggtct agctccttat ccattttctt
    18061 tagtggcttt attaaatgcc aattacgcag acggaatcga tgtcagctgg atttgggatg
    18121 gtaattttga aaactttgcc gatatggata tcccagctgt tatttctgga ggagaccgtc
    18181 atacagatat gactttacgt ctaaaagttg ccgggattcc agaaaataaa ttgacagaaa
    18241 caaaagaatt accagaagta atcgaaaaaa tcaaagaatt gccaactgag catgtctata
    18301 ttttagctac ttacactgct gtattgcagc ttcgtaaaga actagctgct caaggattta
    18361 tcaaaggagg aatgaaccgt ggctaattat gaactcaata tcgcccacct ttatggaaat
    18421 ttgatgaaca catatggtga taacggaaac cttttgatgt tgaaatatat agcagaaaaa
    18481 atgggggttt cttgccatac ggagatcgtt agtatacatg aaccttttga tgctgataaa
    18541 tatgacttgg ttttctttgg cggtggtcaa gactttgagc aattaatcat ttcaaaagac
    18601 atccagaaaa agaaagattc tttgatcaac tatatcgaaa atgatggcgt catgttagcc
    18661 atttgtggtg gctaccaatt gctaggtcac tactacatgg gagctaatgg agaaaagatc
    18721 caaggaatca gtgcactgga tcactacacg ttaagtcagg aaaataaccg gtttatcggt
    18781 gatatcgtta tccataacga agaattcaat gaaacttatt atggatttga aaaccacaat
    18841 ggtcggactt tcttaggtga aggtgaacga cctttgggga aagtggtaca aggtcaagga
    18901 aataatggtg aagaccaatc tgaaggtgtc atttaccgaa atgtcttcgg ttcttatttc
    18961 catggaccta tccttgcaag aaatgagaac ctggcagctc gtttgatccg cctagcttta
    19021 gaacaacgtt acggtaaaga attggatctg ccctatcttg aaggacaaac agcctagtta
    19081 ttcgaaaaag gggaatgtga caaaaatttc gtcacattcc cctttttcgt atcctatttt
    19141 aagcgacttg cttggcataa cggttcacgt tttgcttgat attcccgatt gttttcggat
    19201 cattgattcc tgtgacttct tgaatcaatt gttccaagtt ttcatggatc ttcatttcat
    19261 gcaattgacg gctttgttca tcttcaggat catagtaatt aaaaataatc cccattgttg
    19321 ccgtcaaatg tgggcaagtc aagttatgtt cttgtagttc tctcactgga cgtgtaaatc
    19381 tttcttgata acctaactta cgtaatggtg tccgagcgac acgtgtgatc gcatcagaga
    19441 tatttttgtt ttggaagcga tgaatgattt tttcaatata cgcatgatgc tgctctgcat
    19501 caaaccccca tttggcaatc agcaaacttc ctgtttcctg caaaactgat tttaattgtg
    19561 ccacaactaa cgcatcttgc attgcttcat caatggtctg gtaacctaac aaggcacctg
    19621 tataagcgac agtcgcatga ccagtattga cactaaatag tttgcgttcg atatatggtt
    19681 ctagatcatc tacatataaa acccctttta attggatttt tttatttttt ctttggctat
    19741 cgtcaatgac ccattctttg aaaggttcca cttggacgaa taaaggatct tcatggtgtt
    19801 gtaatgggac gattcgatcg actgctgcat ttggaaagcc gatatatttg tcagtaaatg
    19861 atgtttccgg aagatattta tacacttctt tttctagaaa tgctgaaccg ccaatcatat
    19921 tttcacaggc aatgatatct aaagatgttg tgcttccttc cttttctctt tcttggatac
    19981 cttttgcaat caactcagca ataaaaggta agatattcgg gccaatggcg gtagtaacta
    20041 aatctgcttc agcgatttct tggattactt tttctggatt tgtttgattg ttgattcctt
    20101 tgacattttt tacagtgatt ttttcttttg attcatctgc taattcgatt tcgtattctt
    20161 ttctttcatt taaagcatca atgatcgtgt gattcacatc tacgaaagta atctcaaatc
    20221 cattagctgc caaaacctct ccaataaatc ctcgaccgat atttcctgca ccaaaatgta
    20281 ctgctctcat ttttctctct ccttaagcaa ttgctagatt ttccgttact tcttctttgc
    20341 tgagcgcgtc tgctagctgg acaacattgt ccatatcact gcaataaagt gcgatttgct
    20401 gtacaagttg caaatgctca tctccgactc ctgcaatacc gaacaaaaca gtcgcaatct
    20461 tttcttcttc ttcggtacca aaatctacac catctggtac ttgaacgaca cagatgccgg
    20521 attttttcac atattttttt gcttcatctg ttccatgagg aatcgcgata aaattcccca
    20581 tataaacaga taacattttg tctctatcta ccatggcatc gatatattcc tcatcaacgc
    20641 accctgcttc tacaagcatc tgaccgcaat agcggatcgc ttcttctttg gttttaaaag
    20701 atcgattgag ctcgatcatg tcgaatggca aactttgcat gctgttttcc tcccttaagc
    20761 tgtctgtttc aaatattgtg caatcgattc gtatttttgc ggattgacca tgctgttgac
    20821 tgataaatga atggattgag gtgcagccat ctgtactttt tccgctaaac tttctttggc
    20881 tacgatcagt gaatcaggtt catcttttaa atcttggata ttgacttttc ttaacgggat
    20941 agttaaatgg ttcttgtcta atatatcttt gagcacggtc atccccattg ttgctgatcc
    21001 ggaacgatca tcgtggacaa gtagaatctc tttgatttgt ttgatgtttg ctgaaagaaa
    21061 ctcttctgct ggctcattct caatttcaga aactgatgtt ttctgcttgc tgcttaattc
    21121 ttgaatgatt tcatcatatc gaggtgaatt caaaaagttt tctactgaga caaaacgcgc
    21181 gctcggtgct ttttgttttg ctctttcttg tagttcgacc tgagtaacga tcaatgtatt
    21241 cgagtcatct gtcagttggc tgattgcttg attggtcacc gtttgaggca agtctgcttc
    21301 ctgtactttt tttctcaaga tcgaagcgcc catcgcactt gatcccattc ctgcatcaca
    21361 agcaaaaatg attttttgta cgccagagaa tgtagctcct tgaggaatac cttgggcacc
    21421 tttggaagct tgcttagctg cctttgtctc ttgaacagat ttttcgaagt cttcatttgt
    21481 atctttcgca tccgctttca aaattactgc agaaatacca aacgaaacag ctgcggcaac
    21541 aatcacgccc aaaataacag gaaggtaaga attgaacggt gtcatcgcta gaatcgcaat
    21601 gatcgaccct ggagaagctg gcgctcttag tccagcatcc atcaattgga aaatgaaact
    21661 tcctgacatt cctccgccta taactgataa gaataataac ggtttcatca taacgtaagg
    21721 aaaatagatt tcatggatcc cgcctaaaaa gtgaatgatc atggctcctg gtgctgaaga
    21781 ttttgctgct cctttgccaa acaaagtaaa agcaagtaat acgccaagtc ctggaccagg
    21841 atttgcttca agcaagaata gaatagattt tccagcctct gctacttgtt cagttcccaa
    21901 tggtgtcaaa atcccatgat tgattgcatt attcaaaaat aagatttttg ctggttcaat
    21961 aaaaatactt gtcaatggaa tcaaatgggc agataaaatg atctctacac cttttgccat
    22021 tgcttgagtc aatgcatcga caactggacc aatcgcagaa aaacctaata acgctaatcc
    22081 aaaaccgata agacctgctg aaaaattatt gactagcatt tcaaaaccgg atttgatccg
    22141 tttttggaat agctgatcaa atttcttgat ggcatatcca ccaagaggtc ccatgatcat
    22201 cgcgcctagc atcattggaa tatctgttcc gacaatcaca cccatcgttg caattgcccc
    22261 aacaactgat ccgcggtcac cagcaattac ttttccgcca gtataaccga tcaataaagg
    22321 aatcagataa gttagcattg ggcttaccat ggtagcgaaa gcctcatttg gtaaataacc
    22381 atctgggata aatagagccg ttaatacacc ccaggcaatc agtgctccaa tatttggcat
    22441 gaccatgctt gataaagtac tgcctaattt ttgtaccctc gcttttagcg aaatgttgtt
    22501 tttgggaatc gtccgttcca ttttgtctct ccctctctcc ttttgatact ctaattgtat
    22561 ccgcttacta aaaagagaaa tagtcgtact ttggcacgaa actttgtgcc aaaggtttga
    22621 gtatgtcaac aaaataaaaa gatttcggta tcagaaaact tcaattttaa ataaaactgt
    22681 tattttaatt tttttataat atgcaaacaa taacttttgt tagcatttaa tttagatgtt
    22741 ttccattcta ttatgttatt aaattttata ttggaggcct atctatgaca ttactgatca
    22801 aaaacatgaa catgaaacgt ggtaaaaaac gtgtgattcg acaattgaat cttttagtag
    22861 aatcaggaga agtagctgct ttgattgccc cgaacggatt tggcaaaaca actttgctga
    22921 atggcattgc ccaactgctg ccaacaactt atgatcaatt gtcccttcac aacatctctc
    22981 ctactgctta tacagattat cgccggtctt tcttttttct agaatcaacg aatcagttat
    23041 ccgactatct atcggctgtt gattatttga ctttgatcaa atactattgg cagagcaaag
    23101 tagaatgcca aactgttttc caacgattga agatgactga gtaccttact ttgccgattg
    23161 gaaaaatgtc tctaggaatg aaacaacatg tcttgcttgc tgcttacttg atcagtgatt
    23221 catctgtcat gttattcgat gaaccgttga acggattaga cccaggaagt attgatatct
    23281 tgaatagcct catcagacaa tggcagaaag aggggaagac gattcttctt tcttcccacg
    23341 ctatcgcgaa tatccaatcg atttgttcta aagcatgttt tctaaaaaat ggagaaatcg
    23401 tcacagaatc ctcaaatatg gaagagattt tagctattta tcgtaaatta tatgaggtgt
    23461 aactatgtct ttacttacat ttgaacgcaa tctcttacta aaaaacaaag taaacttttt
    23521 atttcctcta ttgttggtcg tgctctttgc attcccactt ttttttgatc ataaacttgc
    23581 ttatacagaa ttcgatgaac tgaatcataa ctatgaagag atgcaacgtt tgattgaaac
    23641 gttgaaagaa gatgagaatg agaaagaatt tgtggaaagt ttagagaaaa gcaacaaact
    23701 gatcgaagca atcttacatg caaaaaacac agggaatgtc cagcaaatgg tcgaagcaac
    23761 ttatcattat gaaaaagata tactggatcg actgatttct ggtcaaagac aaggaattcc
    23821 aatcattgaa caacaaaaac gtgtcgagtt gctccgttat atgaaagaac atcagatcca
    23881 aagatattct atttttgact tgccagcgca tctctcttta gcaaattact acgaaaatat
    23941 tttttcagga atgatttcta gttttttgat tttatgtata accgccttgt ttctttcttc
    24001 catcatctct tatgaaaaaa gaaagcaggt aatctctcta gtcaatctat taccggatag
    24061 tatggtaaag aaacatagca tccgttttac catttattat ggtgcagcta tgctaagtct
    24121 cgtgatgcca tttttgatcg tctctatact agtaataatc aaaaatggtt tgggagactt
    24181 ccgttatcct gtaggaacga tcattggtca ggagatacga atcctgccga tgtatgagta
    24241 tcttttccag agctttctgt tcctgttgtt gtgggtgctc tttttaagta caatcagttt
    24301 tcttctatct gctttatttg aacattctct agtcaatctt ttaggtacac ttctttgtct
    24361 tttcttagca gagtatcgct tgttttcttc gattggatgg atagaatcaa tcagtcatta
    24421 cttgccaact tcttatgttg attttcaaaa tgtgatcata ggaggagata ttttctcgcc
    24481 actagcttca gaacaggtta ctttcatgaa tggtatcttg acacttggta tatggagtat
    24541 cgttttatta ttcataggta tgggcactat ctatataaaa aagagctatt aaaacacaaa
    24601 aataataggg gcggtgacaa aaaccttgtc accgtcccct atttcaaata aacggttttc
    24661 tagaataaat agttttcaaa agcttgcatc cgcagtaata agaacctccc aacaaaaata
    24721 gctcctctta tttttatctt ggttgaagtt attacttgat gcaaggcagc tttttttact
    24781 gtgaacattg gttatatatg tccatgttca aaaatcattt ggacttcttc aatggtttcc
    24841 gcatcactga gcgccattac ttggtcgatg tctgaacaga aaaaggctaa atcttggagc
    24901 accgctaact gatgttcttt ttttaaagcc actacaaata agagtgtcgc gatttttggt
    24961 tcctcttttg ttccaaagtc cacaccatca ggcacttgga ctaaatagat tccttcttct
    25021 actacttctt catctttgtc agtcccatgt ggcaaggcta cgaaattccc gatatagaca
    25081 ctcgcttttt cttgtctttc gatcattgat tggatatatg ccggtttgat tcccccttgt
    25141 tgggtcatcc actggccgat ctcggtcaac gcttctttcc aattttgata ggattgatcg
    25201 agcaatattt tttctgctgg tagctgtatc atcttttcac cttcatttct ttatttcttc
    25261 aatcaggatt ttcgctaaca actgataaac gatttcttta tttcctgagc ggaagatctc
    25321 ggtgttcaag tcattcatga tgattgctcc actgatcttt cctaaaagaa tcccgtcttt
    25381 ttcttggatt ggcgaaggcg ccagcataac taacatacgg gacaaatcga ctggctgtct
    25441 atccatccct tcaatggtca acggatgttt caaattgaaa atacaaaaga tcggtttaga
    25501 aatcaatgag ctggatgtat ggaataaccc catatttgta tgaggaatac cgataggtgc
    25561 ttgttcatat cgcttaagca gccggcgatg aaccacctct tcatccgtta ctagtccttc
    25621 aggaaaataa ggaaataagc tgcgaatcgt ttcggaaatc gtctcaggat tatccagttc
    25681 ttggataaaa aaacgaccga gtaattgatt gatttgatcc atgaaatgca ttgtttcctc
    25741 ataagtatct tctatcatcc actcgttatt gctgttatac gtaatttttt gccttttttt
    25801 cttctcaata tgcgaaaaag cttcttttag ctgcttgact tcatcttcta gcagtagcgg
    25861 ggagactaaa atatattttc caccgtatcc tggtaaaata gatgtagaaa taacaacatc
    25921 atattcttct tgtaaatcga ttttttttaa atcagcaatc cgatagaaat gaatttgttg
    25981 gatgaaggga aaataccttt tcaatcgtag ttccaacatg ctagtagaag ctaatccact
    26041 tggcgaaaat cctgcaatac tgatattgct ttctttcgga cttcgttcca atgaattcgc
    26101 aaaatgaagg accatataag cgatctcttc ttcggataat tttttttctt gaaaaatccg
    26161 ctctactgct ttacggattg cattcgcaac ttcctcgtat tgaaccatga ttttttctaa
    26221 aatcggattg gtcagtgtct cttcttgtaa aacggctctt gaaaacacac cagataaatg
    26281 agtcaacagc attttgtaca aagtttcatc ttcataaaaa tggacattcg tctcttcact
    26341 tacttcttga atcagttgtt ttactaaata agcaagctgt gtatcgaacg tctcttcaaa
    26401 gaaattctga tcaaatgagt tcgtgaagtc atttaataat cctgcaaaaa agacgatctc
    26461 atttactgga taaagttgtt ttgtctcaga agcgatccgt gaaaacaatc gtttagctgt
    26521 ctgtagtaat tctttgtttg ccagactatt atatgtctca tttgtcagta aatgattttt
    26581 cgtcacacgc tccatcgtca atgtcagcat caaaaccaaa tgttcaagct ttctatcact
    26641 tagattggtc tttttttgga tcaattcttc cccgattact ttgcgtgcaa gagatagatg
    26701 ttgcggatca acgaatcgaa aaaaataatt cagtgatgca tcgaaagaaa tactatgaaa
    26761 aagttcatat tcattgattt ctaattctaa tacattcgct gtcagaagcc ggcggtattt
    26821 ttccggtcct tcgatctcgt atccctggtt ccgaatgatt tccaaaggca gttggcgaat
    26881 actttcttcc aactgtttga tatctgcata aaaagtggta ttactgatgc gatatgattc
    26941 catgaaatga gcaagactca acggctctgt cgtcagtaac aactcggcta aaatcgcgtg
    27001 ttgtcgttta gcagtggata agtcattact tttcccatct gtattttctt ttaattgctc
    27061 taatacttct tctgatgcct caatgacaaa tgagcctcta gctgcttttt tcaaagaagc
    27121 atttaccgat ttcaatgaga cttcaagatt atctaattcg cggtaaatag tccgtttact
    27181 gaccttcaat aaattcatca ttttttggat agatacaggt gacgggctac tgagaaattc
    27241 tgctaataat tgtttttctc tgggcgataa atacatctta tatttctccc ttcacgagtt
    27301 tttcgtattc attcgtagct acccaatgtt caactagaac tagccgaggg atcttttctt
    27361 ctagctgttc agttacagct agttctcttt gtgcgatata aatcgtttct tcttggattt
    27421 tgattttttc tattgattct tttgagataa caaactgttt tttcgctttt tttgctaatt
    27481 gacttaaaat cgtcatgccc atagtttgtg agccgcgtct gttgttttta tacaaaaata
    27541 caatattagc aggcgacgtt ttttgttctg tgatatctgc tatctctttt ttttcttccc
    27601 gctttggaaa ttggatcgct tcttttattc gtgacagcaa gatatctgtt tccaaaaagt
    27661 gatccagtac atagatttga ctttgaggta ctttttctct tgcaagaccc gctagttcat
    27721 gttgcacgat aaccagctga tttacttggt tattcaattc atagatagag ctgtatccga
    27781 cagaaagatc gattttttct gctgctaatt tctttttcag cagggcggct cccatagcac
    27841 ttgatcccat acctgagtcg caagcaaaaa tgacggaatg aatgatagat ggttccaaga
    27901 tttctcccct ttctttgacc gttttgttct ctttttcttt atttggtaat ttcaatagta
    27961 tcgctgcaca aatgaaggtc acaaatgtac tgattgctat ccctatactt accccagcta
    28021 gttgtggata aggcgcattg gcaaaaatca cgagtagtga cccaggcgat acaggtgcag
    28081 ctaatcccgt atctaaccat tgaaatatca acgtgccgct tgccccacca gccattacag
    28141 ctaaaaatag atacggtcgc agcaagacaa atggaaaata gatttcgtga atcccgccaa
    28201 tcgcatggat catcaatgct ccactcgcat ttactttttc ctgtttttct gcaaaacaaa
    28261 ggatagaaag taagatccct aaaccaggtc caggatttgc ctccatcaaa aataaggaag
    28321 agtacccgac tgtttgtgag aattcgattc ctaaaggtgt caatacacca tgattgattg
    28381 cattattgaa aaatagtact tttagaggtt ctaagatcag actgacaagt ggtaacagat
    28441 tcattgtgac tagccaagac acaacggagg cgagcaacaa gtgaacacta tttaaaactg
    28501 gttcaataaa aacgattcca attatacaaa taacgctacc agtcagtcct actagtaggt
    28561 tgctgacaag catttcatat ccagccttga atttcacatg gaatctagaa acaaattgtt
    28621 catatacgat gccggcaagc ggcccgataa tcatcgctcc tacgatttgt ggagcattgg
    28681 aacgagcgat caaccctaaa gaagcaattg caccggcgac tgcccctttt tgtcccccta
    28741 ccgatttccc gcctgaataa ctgatcaaga tgggtaaaag cagctggatc atccaatggc
    28801 atacctcttc taatgtggtg tgcaaatcgc cagacacacc ttttgttgct aatgacaaga
    28861 ttccccaagc gataaacaga cttagatttg gcatgatcat ttcatttaag tgatgtccag
    28921 cttttctcag ttgtttcata gacacgcgac tgctgttgtt tgctttccat ttgtattcca
    28981 tctttttcac cagacttcta tttattgatg gacggatttt atgtagaaaa agcagcaaac
    29041 gaaaaaccgt ctgctgcttt cagctatctg ggaactattt gcctggttca gacgcaatga
    29101 ttgctagttc accttggacc gcccactcag aaacgccatt tggaataata cagcttgttc
    29161 ctttttctaa tggatatgtt tttccgtcaa cgacaaggtc tcctgctcct tcgattaccg
    29221 tcatcaatgt ataaggggct tgttttttga aagatgtgat tcctttaata tcccactcat
    29281 atacattgaa aaactcagtt tctaaataag tgacgatcga tgaattccct tttcggactt
    29341 ctttgatttg taattctggt gttttcgcag ggacagttgt tacatcgatc gattgttgga
    29401 tatgcagctc tcttgtttgt ccttgcgcat cttttctgtc gtagtcatac acgcggtaag
    29461 tcgtgtcaga actttgctgt gtttctaaaa tcatgattcc tttaccaata gcatggatcg
    29521 ttccgcttgg tacataaaag aaatcacctt ttttaactgg tactttcttc aaaaggtcat
    29581 cccagcgccc atctttgatc atctcagcta gttcttctct tgtctttgca tgatgaccat
    29641 agatgatttc tgcaccaggt tcagcatcga tgatatacca gcattcagtt tttcctaatt
    29701 caccttcatg tttcatccca taggcatcat ctggatgtac ttgtacagat agatcatctt
    29761 ctgcgtctaa gatcttgatc aataatggaa acactggttc tgaaggatgg ccaaacaatt
    29821 ccttgtgttc tgaccatagt tcatctaatc ttttcccttt gaattcgcca ttttctacta
    29881 cactgacacc atgaggatgc gcactgatcg cccaatcttc tccgatttta tcgttaggga
    29941 tatcaaaacc aaagactgaa cgtaaacgac ttcctcccca aattttttct tgaaaaactg
    30001 gttttaaaaa cataggttcc tgcataattc aaactcctct catttttcat agctattata
    30061 gcattatttc ggtaataccg ctttctcttt caaaaagttt tcaacaattt catctcgaca
    30121 tatgagatac tccgcacgat gatcgacagg atgtacgcga tttgacaaaa atatcaacgc
    30181 ttcttgtgtt tttatatcaa tcaagaaaaa cgtacctgta taaccagtat gaaaaagaat
    30241 cgcatgaccg tcttctacat ccttttttaa gtcccatcct aaagaacgat agccattttt
    30301 tttcggtgtt tgatcttgca gtaaagaatc aatcgtctct tcttctaaaa aacgaccatt
    30361 acggattttt cccttgttca aatacatttg gacaaaagta atgctgtctt tcaaatcaat
    30421 aaagagaccc gcgttacctg catgttcagc caaaactctt gctttcggat catgagtttg
    30481 tccacgcaaa acctcacccg aaggcagttg ttctgtggga acgatttttt tggaaggaaa
    30541 aggaagaaat aagctgttca tcattcctaa tggttccaat acttgttctt gaaagatttc
    30601 cactgcagtt tcttggtaaa tttcctctag catcaaccca agaagaatcg taccggcatc
    30661 tgtatatttt acttcttttc ctagttgctc acctggttct agcttcaaat aagcggcgat
    30721 caattcttcc tgagatagct tatctctgtt tggtatccat gttttgatat cagaagtatg
    30781 tgtcagtaaa tgacgcaaag tgattgtctg attttcaaat tcaggaagat atttatgaag
    30841 cggttgatcg agttctatct ttccatcttc taatagacgt aaaactaatg aagtggttgc
    30901 aacgactttc gtcagtgagg ctgcatcaaa caacagatct tctgtcatca gctgcttttc
    30961 aggaacgact tgggcaaagc cggctgtaaa tgtttctact tgattttctt ttatgaaggc
    31021 gactgctaca cctggataaa atccttcttc catctttttc aaaataagct gtttcgtgtg
    31081 tggatacatc tttttctcct gcttttctat cagattctta cagtccaagc ttaaagctta
    31141 atctatgagg aatcaacctt ttttacacaa aaaaactgcg cgaaaaaccg cgcagtttcc
    31201 cccggtatac tattttctct ttctatctct agaataagcg atagtatgac cgttcataaa
    31261 atgacgagac atgcgacaat cgttaagaaa gaaaaagtta agcgtcgcat cgaatgtcag
    31321 ttttattctt ttcttttggc tcactgacga accaccattc gattcctata gcatcataga
    31381 ttgtgagtac tgtgtgcttt ttgtctaaga agaaatcaac tgatagttgc tgcaagtgag
    31441 caatcagtcc ttccatgctt tcagtatcga tcatgaattt cagaaaatca agtccaatca
    31501 cttcatctgt aggcaaatca attgcgcctc cgctagcttc tgtcaaacca acatggaaat
    31561 ctccttcatt caatacatgg atgccctctt cttcattatg taccttcaaa ccaagtacat
    31621 gattaagaaa ttggcgttct tttgttaagt tagtaacatt taaatggatt ttttcaaaat
    31681 aagaacctgt tgatagttta ctgaattttt cttcagattt tcttaataat gcttcttgat
    31741 ccattgtttc tggaatttct ctttggtcgt tcttattctc ttcatggtaa atttccaatt
    31801 cattctcttc tgggtctacg agcaaaatcc ccatacgtcc atgatcttct aaagcttctt
    31861 tgattggata attcatttta cggatacgac aaaggatatc tgccatttct tctgtgctag
    31921 ggataatcaa tgaaaaacgg ttcagctttt tgatcacccc catgtgatca ttcgctctag
    31981 gactttcttc cagcagcaac atttcacttc cagcttcttc tgttcctaaa atggctaatt
    32041 cgttttcttc tcttttcaaa tcaaagccaa caacgttctt gtaaaaatca atcattttat
    32101 ctcggtcttt cacccgaatg gctacacttt ttaaataggt ggcagccccc aattgaaaat
    32161 ctttcatatt tcttgctccc ctctacgtca agctttattt taacgatgga ttcttcttct
    32221 ttcaagcaat aactttgatt ttgttaaaaa aaatgtgaga aaattcgcat tttgcttgtt
    32281 ttttgtaaat taatgagtaa aaactcttat aaaaagaggt tgtgaaaaag ctgttctgca
    32341 tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt ggttgggctt
    32401 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa aaagtcgctt
    32461 agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg cataaatttg
    32521 gcttaatacc gagaaactgg cttttgaaca ccgtttacca agaggttgtg agcctgccgt
    32581 ttaactccga gtcacaagga gcaatttcaa aaaacagctt ctcaggtttg ttgactattg
    32641 tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa ggaagaggtt gtaaaatcag
    32701 cggtttgacc tgagtattag agatacaaaa cggaaaaata gcttctcata ttttgccgtt
    32761 ttttgagcta ataccgaagg acagctgatt gaacaccgtt tattcggaaa aagggactgt
    32821 gacacgactt ttgtcacagc ccctaaaaaa aataataaat ttgtactatt ctatgaagct
    32881 tcttcttcag aaaactgact gttataaaga tctgcataga atccgtcttt ttccatcaat
    32941 gtatcgtggt ttcctgtttc tacgatcgaa ccatgattca tcacaataat gttgtctgca
    33001 tcgcgtattg tagacaagcg gtgagcaaca acgaaacttg tccggttttc caataggcgg
    33061 ttcatcgctt tttgaatcag tatttctgtt cgtgtatcta cactagatgt tgcttcatca
    33121 aggatcagga catctggatt tgctaagaat gcccgcgcaa ttgtgattag ctgacgctgc
    33181 ccttgcgaaa tattgcttgc atcctcgttt aatacagtgt tatatccttc tggcaattta
    33241 cgaacaaaat cgtctacgtg agcagcttta gcagcctcaa tgatttgttc gtctgttgca
    33301 tcgtcgttcc catatttgat attgtcatag atacttcctg taaacagcca agtatcttgc
    33361 agtaccattg aaaactgtgc gcgcaattca tcacgtgaaa gatcacgggt atctacacca
    33421 tcgtaacgaa tactacctcc agaaacatcg tagaaccgtt caagaaggtt gatcagtgtc
    33481 gtcttccctg caccagtcgg acctacaata gcgaccatct caccaggttt gacatccaag
    33541 ttaaaatcag tcattagcaa gttatctggt gtgtagccga attgaacatt ttcaaaacga
    33601 actttgtatg gtgtatcttt ttcttctgga aggccagatt tttcattgga catctcttct
    33661 tcatccaaca cttcaaatac ccgttcggct gaagcaactg ttgattggat agtgttcatc
    33721 aaacttgcaa gttgagtgat tggttgagag aactgtgttg tatattgcaa gaacgcttga
    33781 acatccccta atgtcatgtt cccatttgca acttttactc cgcctagtac agcgacgaac
    33841 acatagccta gatttttaac gaaattcatc aacggcatga tgattgccga aataaattga
    33901 gctttccaac cagcggcata cagattctga ttttcttttt caaatacttc ctggtctttt
    33961 tcagtatgat tgaatgtttt aacaatggta tgaccaccat atgtttcttc tacttggtta
    34021 ttcaataaac ctaaactttt ttgctgatct gcgaaaaaca cttgtgattt cggcgctacg
    34081 atcatcacga tgatcaagct taaagggatc atcaataacg cgactaacgt caattgccag
    34141 ctgattgaaa gcatcatcac gatgacccca atcaatgtga ctgcactagt tacaaattga
    34201 gtcaaatttt gctgcagcgt gcttgcgatg ttatccatat cattgattgc tcgagacatg
    34261 atatccccat ttgtatgtgt atcataatag gaaatcggta cacgattcat tttggcttcc
    34321 aaatcatgac gtaattcata aactgttcgt tgggaaacac gtgtcataat aaattgctgt
    34381 aaaaagttga atacagcaga aatcaaatac atcacgatta ctgtcgcaat gatctgcgcg
    34441 attttatcaa agtctatcgg aaattttgtt acttgttggc cttgttgcat ctgttgagcg
    34501 ccagccatta cacctttgaa tatttctgtg gttgctttcc ccaagacctt tggtgtttgg
    34561 atttggaaga tcacggctgc tattgctagt acaaatacag cgatgatcgc aaccatacgt
    34621 ttagacatat agccaaacaa tcggcgtacc gttccccaga aatttttagg cttttcacct
    34681 tttacaccga gatttctgcc aggtccacca tgtcctggac cgcctgctgg tccatgagag
    34741 ggacgtgtgt ttgttttact catgctaagt cctcctctct tagttgtgaa tgaacgattt
    34801 cttgataagt ttcgtttgtt gctaatagtt cttcatgtgt tcctttgcca accagttttc
    34861 cttcatctag cacaaggatc atatctgcat ccattactgt acttactcgt tgagcaacta
    34921 aaacggtgat actttctttc atattcgttt tcaatgcttg gcgaagattg gcatccgttt
    34981 tgaaatccaa cgctgagaag gaatcatcaa acacatagat atctgctgtt ttgactaatg
    35041 cccgtgcaat tgccagacgc tgtctttgtc caccagagaa gtttcctcct ccttgttcca
    35101 catggctatc taagccatct tccatctctg aaacaaaatc tttagcttgt gcgatttcta
    35161 atgctttcca aatcatttca tctgttgcat ccggcttacc atactgcata ttgtctcgga
    35221 tcgttccaga gaacagaact gctttttgag gaacaaagcc cattaattct cgcaaattat
    35281 attgagtcat gtcactaatg tccgttccat ttatttggat cgaaccagat tcgatgtcat
    35341 ataatcgagg gatcaaattg actaaggtcg ttttacctga accagtcccc cctatgattg
    35401 cgacgatctc gcccgacttg gcttggaaat caatatcttc caatgcaagt ttttctgctc
    35461 catgatatcg ataattgaca tgattgaagg ccaatgttgc gttttttcca gcaaaagaaa
    35521 ctgttttagg attttcaggg tcatggatac cgattttttc atccaacact tcattaatac
    35581 ggtcagctga agcttgtgca cgaggcacca tgataaaaat agcggaaagc atcataaagc
    35641 tgatcaagat ctgcatcgca taagtcataa atgcaatcag attaccgact tccaaagtca
    35701 tatctgctat gtagtgtcca ccaaaccaag tgattgctac gtttgtaccg ctcatgatca
    35761 atgtcatcat tggcatcatc aatgcgacga tcgtattaac tttgattgcc gtatttgtat
    35821 aatctttatt cgctaaatca aaacgatttt cttcaaagcg tgtcttatcg aatgctcgaa
    35881 tcactctgac ccctgtcaat ccttctcgaa ataccaggtt taaacggtca gttttctttt
    35941 gcatactttt gaataatgga acagcaaagt acatgattcc gccgatcaaa ataatcatta
    36001 ctgggatgac aactaagaag atttttgtca actgatgatc tttttgataa gcaagtatgc
    36061 tcgcgccaat caacgtgatt ggtgcattga tcatcatacg taaaaacatt tgtgtaacca
    36121 tttggatttg agtcacatca ttggtcgttc ttgtgatcaa agaggccgtt ccatatttat
    36181 caaacgcatc ttttgtcaga ctctctgatt ttttatagac atccgtacgc aattgcttac
    36241 ccagtttctg tgattcacga gtagcaaaaa atgtatttgc gatagcagct aagatactga
    36301 tcaaagaaaa gccgatcatc acaaaacctg tatgccaaat ataatcgaca tctccttgcg
    36361 ctaccccttt atcaataata ttggaggtaa gtgtcggcaa atataaatct gccagtactt
    36421 ggatcaccat gaaaactgca gctgcaattg ctgaagtcaa ggagatccgt tttacgattt
    36481 ttagcatcta gttcccttct ttctatttat atactccgtt tttcagccaa gaaagtttca
    36541 aattgaattc actgattgct tgctctactg agtaatcttc agatgttgaa aataagcgat
    36601 acgaatgcgc aattgttgta aatacagtat tcatcactaa ttggatcagt aattccaatt
    36661 catgatcatt ttttactttc agcttcgtcc gatcgatcaa ttcaagcaac tcttttgcat
    36721 cttgtttgtg ttctttatgt ggatcgtgag gcgttttttg gcgactcgta tgaggagcga
    36781 cttttcttga ggcacggtaa tccatgttca taaacaagtt acgataaaaa gaagcattct
    36841 cgccttccaa aatctcataa atcattttag aaaaatagga ttcgaatcct ttgaatagat
    36901 caccatcgga ttctttcatt agttgtaaca tatctttaga gctgtcacgt ctcattgtct
    36961 caaagtaata aaaataaaga tcctctttat cttcaaaata ctgatagaaa ctgcctctag
    37021 ggatatccgc taatttgacg atattagcaa tcgaagcttc ttttaagggt actctagaaa
    37081 actcgattct cgcagcctcg attagtcttt tttgtttttc tttaggcaaa tggaaaaatg
    37141 tctgtgtcgg catatggatt ctcctttctt tcaagatgac acattgtcat atgacgctat
    37201 gtcattataa cacctcctac ctgttttgca agacatttgc atagtttttt tcattatcat
    37261 ccgatacatt aaaaaagtga taagaggaat ccttccattt gaattaatga gagttgccca
    37321 ttaaaatagc cacatactaa acaaaggatg agataaatgg cccaatcaat tagtgtccaa
    37381 gacttcaata aattgcctct cgatgctgac agcatcgttt tagatacaag aaatacctta
    37441 gattacacaa aaaaccactt aagccactcg ctttctatcc ccgctgctat cttgccaaga
    37501 caattaaaaa atctagatcc aaagaaactt tattatattc taagctatac aggcaggcga
    37561 tcagaagcat tagccgatca acttactgct aatggttttc gtgctgttta tatcattggt
    37621 gggatggaag ctcttcagtc gagtgttgct tgaatattgc ttgttagatt caaaagaggc
    37681 tgtgacaaaa aatcgtgtca cagcctcttt tataccctgt ctttttttag ttatttattc
    37741 ttcaacctta tcaatatgac ttgatagcct tttctttttc gagtaaactg attttgttag
    37801 tcatagttat aacaatcata gcaataaaag gagaaggtaa gcatatggca tctgttttag
    37861 tagtcaaagg tcatccccta actgcagaag aatcgcgcac agttaaggca ttgacgtctt
    37921 ttttaacaag ttataaagaa aatcatccag atgatgaagt gactgttttg gaactttatc
    37981 gcgatgatat tccggaaatc gatgaagaat tattatcagg ttgggaagca ttacgtgctg
    38041 gtgcagaatt cacttcctta tcagaaagtc aacaacaaaa aattgctcgt ttcaatgaat
    38101 tgactgatca gttccttgct gctgataaaa tcgttatcgc aaacgcctta tggaatctaa
    38161 atatcccaac aaaattaaaa gcttggttcg ataccgtcaa tgtagctgga aaaacgttcc
    38221 gctatacaga aaacgggcca gaaggattag tgacaggtaa aaaagcactg cacatccaat
    38281 ctaatggcgg tgtatacaac ggacaagact ttgcctctca atatgtaaaa ggtattttaa
    38341 actttgtagg catcgatcaa gtagatcagc tattcatcga aggaatcgat tatgaccctg
    38401 accgtgcaga tgagttgatg caaaatgcat tagataaagc tgctgcttta ggtaaatcat
    38461 tttaaaaatt gagataaatg catacaaaag agacagagga aaaaatcttc aaagcagcat
    38521 ttgtgccttg gatattttcc catctgtctc tttttgttat tctttctgct ttttcaacgg
    38581 attttcttca taaagagaag agattgttgt gtaccattca aaaacatgag taatcactac
    38641 tttagctgct gcataaacag gaatccccaa gatcacaccg atgattccga acaatttccc
    38701 agaagtcagc agaactaata aaatcgttac tggatggatt gctaactgat tccctagtac
    38761 taatggagag atcaagcgtc cttctattgt ctgctctact gcaaaaacga tcagtacttt
    38821 gatgatcatg aatggacctg ctacaatggc taaaaagata gccggaatca tcgctaaaaa
    38881 tgatcctaag taagggatca ggtttaaaaa acccgcaata atccccaacg tgacagcata
    38941 ctctaaacca atgaccgcaa aaccaatcat aaacatgatt gccacagcaa aagccacagt
    39001 tagttgtcct cttatataag aagaaacttg actattcatt tcactcagca cattcaacgt
    39061 cggtttttgc atgcgtgtcg gtaaaaattt gacaaaataa ggtgctaatt gtttaccgtc
    39121 tttcaataga taaaataaga taaacggcat cgtcagcaga gccactaaaa tagtcgctac
    39181 cgcgccgaca aaacttccaa gactctgaac tgtactctta gatatatctt ggatcatatc
    39241 gcctgccgaa ttcatgaact tatcacctgc tgtttccaac tgttcgcgaa attggttgaa
    39301 cagcgggtcc cgcaaaatct cggttaattt attatcaatc gtatctacat attgagggaa
    39361 gttactgatg aagctcactg tttgttcctg aatcttaggg atgatcacta cgctccccca
    39421 tgcgattagt gcaacaacca aaacaaacag cccaatgata ctgtaaagcc ttggtacttt
    39481 tcgcttttca aagtaatcaa ctaccggatt catcaagtaa tacaagattc ctgctaaaat
    39541 gataggcaga cctacgattg ctaaaaattg ccagattggt gcaaacaaat atgaaacttt
    39601 tgtaaataaa aagacgatca gcaatattaa caatacaaca agtaaggctg tgactacttg
    39661 attatttaaa aaccagcgcc aaaaccacga taccggtctg ttcttcttat tttttccttc
    39721 cataattcct cctgttagtt ataaaaaatt tggcttccca aaccaatagc aggcagtcta
    39781 tgcggatgaa gatagattcc atttgcaatt tgtccttctt ctggacaagc atcaaaaatc
    39841 aatgatacgt gagctactgt ctttaaattc ccattggcca tcggaccgac ataatcgatc
    39901 gtatatacct gatcatcaaa agcaattgtt ccgcctgctt tcaaagaaaa aggttcgtct
    39961 tttgatactt cttgaatcac cgaatatttt cgtaaggcag ctgttgctgt tttatcgaac
    40021 aaaatcagca tgggttcttc cttgtttatt gcttctgtcc caatttctgt aatctttgcc
    40081 tgaatcattg cactctctcc tttaattgct ttcaaaaaca gtataacaca ttctcattct
    40141 ttagccgtgt ggaataatta tgtgatagaa taaaaacgaa aactatttca ttacaattag
    40201 gaggaatcag ctcatatgat cgaaaagata attttaggaa cctatacgcg tcgcgaaagt
    40261 aaaggaatct atacgatcga ccttgataca gaaaaagaag aattagccaa cctttcatta
    40321 gctgttgaag aaaatagccc tacctatgta gcaaaaagta aagctggcaa tctttacaca
    40381 gttactagtg tggatggatt aggtggtgcc ggtgcgtacg ataaggattt tcattttttg
    40441 aatgcggtaa cagagtctgg cgctcctttg tgttatgtag cggttgatga agcacgtcaa
    40501 ttactatacg gggcgaacta tcataaaggt gaagtaaatg tctaccatat attaggagat
    40561 ggcgaactga aagcagcaga ttcaatattc catcaagaag cgacaggtcc tcataaaaac
    40621 caagatcatg cgcatgtcca ctatacggat ctgactcctg accaacgatt agtcgtatgt
    40681 gatcttggta cagatcgtgt gtatacttat gatgtgtccg aaaacggcaa attgaatctt
    40741 gtaactacct tcactgcaga gccaggaaca ggccctcgcc atcttgtctt tcatccaaat
    40801 ggacaattcg cttatttatt tggtgaatta gacagcacag tccgcgtgct ttcttataat
    40861 aaagaaacag gtgcttttga agaattacag aaaatatcga ccttgccaga agaattcgac
    40921 ggtgaaaatg gaggggcagc tatccgcatt tcaaatgatg gcaagttcct ttatgcttct
    40981 aaccgaggac ataactcgat tgctgtattc gcagtttttg aagatggttc ccacattcaa
    41041 aacaagcaaa tcatctcaac agaaggggat tttccccgcg atttcgcact agatccaagc
    41101 aataattttg tcgtgtgtgc caatcagaac accgataatc tgacattata tcgccgtcat
    41161 cctgaaacag gattactgga attactgaaa aaagatgtct atgtaccaga atgtgtctgt
    41221 gtttattttg atcgttagta agaaaaggag aatgaaatga atcagcaaga cgtattagac
    41281 agactacggg aagaactaaa tattcctttc tttgatggga aaatcgaaga taaagagtat
    41341 agcgaagagg aatatcagaa actcaaagcc gatttacaga actactttga acaatatgta
    41401 cagaatgtag aaaactaatc agctttccat actatctgaa aagaacatca ggacaagact
    41461 tttccgtcta tcctgatgtt cttttctaca aataaaaaac cgccgagatg gttagactcg
    41521 acggaaaagg agtatttact cgattttgag taagtacaag gttgttgttg gttataggta
    41581 tgtcattatc ataaaggatt ttgatcactc tatcataaag gtaatattac atttatatta
    41641 gataactcta gtttttattg ttatcaaaga tttcttttat tttttcaaca acaaattagt
    41701 cattttttgg atggcagctg tcaattggtc tgtcgcttgg tcttgttttg cttcagatgt
    41761 attgaataga tcttcatcta cccctgattc aagatcgata ctttggatca tttcttcaca
    41821 gcctgctaca tatgaaagat aagctttttc aaaattttta tgaatgccca ttgcttttgc
    41881 tggtggacgc aaagtactga ctttcataag cattgcccga tatttttttg ttccttcttc
    41941 aaataacagg atgatctctt caagagtcga tttgtccagt tcacttgttt ttccttcatc
    42001 aattgcgttt ctgatcactt catatttcgg atgcattcct tctccgattg cttctgtttc
    42061 cttgacgatt tgatcgatgg actgcacata atagccaata ttcggtaaca tgttcgtttt
    42121 cctactttct ccattgttgt tattatttat tctacttgtt tttatcctca aatgacaagt
    42181 acttgaaaaa atttaatggt ttctatacag ctcgccattc ttattcgtgc tcaaagaaac
    42241 catctgctaa actatattgt ttccctgcga caatgtcata ttggatcaat tgatattcgt
    42301 cgtaaatctc ttgttgcgag cgattgaatt gcgcttcttt ataccactgt ccgttttgat
    42361 agtacagttc tcgttcaaac gccggaagtt cctgttctaa tgataaaagc agctgataaa
    42421 agccatttgt tgtctgattc gtctgttcca ttagattcgc tgcaaaataa aacggactgg
    42481 tgattgcatc gtgagtcgtt tgcttttcta attctccttt actgtcgaac ataagaaatt
    42541 gtgtttcatg caatgtttgt ttgtcatttt ttgcttggat cgtatctgaa tagatccctg
    42601 gaagatgatc cccccaaaaa acaactaaag tccttctaga aagattcttc aattcttccg
    42661 tgaactgctt cagtgccgtg ctgctatagg aaatatcttg cagataattc tctaagtcaa
    42721 gtgtcccact tccatctgaa gtttttgcag gatagtctag attatcatac tttccatcgt
    42781 agggcatatg tgtctgcatc gtgactaaat gaatgaactg aggtgttttt tcttccttga
    42841 gaagtgtcat tacttctttg taagctgact catcagaaat atatggatta ttctctatcg
    42901 tatccgtata ggtcatcgta cgctcgctga tgaactggtc aaatcctaat gtctgataaa
    42961 catcctctct tttatacatc gaagtattgt atggatggat cgctgtcgtc tggtatgatt
    43021 gtgcgtttaa cgctgaaacg atagaaggaa gctgatccat ttttggtaca agcatcgtgt
    43081 aaggggtggt gagttgcgca ttaaataacg ccatagaaaa accagtcaat gcttcaaatt
    43141 cgatatttgc tgttccaccg ccatagtttt gagaaagcat attgccacta tatgtttgat
    43201 cagctacttc ataataatct gccaatggct caccagatac ttctacgccg tttaatcgac
    43261 taggatctga gaaactttca ctcatcacga acacgatgtt cggcgactct tcctctacag
    43321 ttttattttt ttcatcggct agcttctggt atttttcagt gatttctttg atcttttctt
    43381 tcgaatatcc ttcaggttca tccattggtt ctaccttcag attgtacaaa aacccaccga
    43441 taaaaccagt gttatagtag ttcattttct ggctgtaagg tatccatagt gccgttttat
    43501 tatatgcttt tcgcaacaaa ttatcgggat tgttgaaatt actgatatac cctagcagtc
    43561 caattgccac aagcagtgtc gttaatcgga tcaattggaa tcgccttcct tttttcaggc
    43621 ttcgaaacat gtaccagcaa aacaatccta tcacggttcc tgcggcagct aagatcactg
    43681 taaacagcat agtgccggtc atatctttca gcaatccgat ttcagtgatc atcttcaaat
    43741 cgtctggata aatcggttct tgccgataac tcattttcat ataatttgca aaacctaata
    43801 caccgatggc tacgacatac gtaagggaac ctaaagggac agatcctgct agtgcaatca
    43861 gaaaaataag aataatcagc aagacgagac aggctaagaa gaatttttcc gtatgccaag
    43921 aaaaagcaaa tttcaacgca aggtctacgg atagatcatt ttgacaccac tgcaaaaata
    43981 aattactaaa aacaacaaga aaaaagatac tgatgattgc tgctgttcct tttaaaatct
    44041 ttttctgttt ggtcgaaagt ctgcttatat atcttttcaa atgcttgctc cttcatttct
    44101 atcaataaaa gaatgttgct tctcttattt tagttgtacg aaattcgaaa gtaaagaacg
    44161 gaaagaagaa ttaatgaaat ccaaatattc tctttcttta tggcctttct ggtttcttta
    44221 ataagcggac ctttcgtact ccagaaattt tttctagctg ttgtttcact tgattgagct
    44281 gttcaaaatc agtactgtct atgtcaatca atatgtagac atgctcattc ttatggttac
    44341 gcgatatatc tgcgatcgct agctcgttct cattcagcag agaaaatatc tctgctaata
    44401 tattggtttt cttttgataa aatatagtga atcgaaacgg cgcttggaaa aacattcgtg
    44461 cggatggata attgaccgct tctctaaccg taccgaacaa aagataatct tttaaggctt
    44521 caactgccaa acgtccgctg tctgccaaag cttcctgagt cgatccccct agatgaggca
    44581 acataagtat acggtcattt tctaagaact cttcttctgg aaaatcagtc aagtatttag
    44641 ccagatgttg tttttccaat gcttgcataa cagcatattt atcaacaatt tcacttcttc
    44701 ctacattgat aagtacagca tcttttttca ttttttctat attcgcctga tcgatcaatc
    44761 cttttgtatc ttctgtcaac ggcagcatga ctacgatgta atccgaggta gaaagaactg
    44821 tttctaaatc tgcttgccga acataatcca actgggcatc gcggatagaa taacccaaga
    44881 catccatccc taagctatag caacttagcg ctacttttgt gcctatcgct ccaagaccaa
    44941 gaagaccgat ggttttgcct tgcaattctc gtcccacata agcacttctt ttgttttctg
    45001 cctgttctaa aatattagga cctgttagtg tttgtaccat acgacttgct tcgattatcg
    45061 gacgggaaga taagagcaaa cagcacaaaa caagctcttt tactgcatta gcatttacac
    45121 ctggtgtatt catgacgatc gtcccgtttt cagaagcttt ttcaacgttg atcgtgttca
    45181 ccccaacacc agctcttgaa attgctagca aatctggtgt aagccattca tctttcactt
    45241 tcgtactacg aataactatt ccttcggctg gcaaatcatc tattattttg aaatattccc
    45301 cttgtaactt ttctatctct tgttctggaa tagcatcaat caaatgaacg tgatacataa
    45361 aaccccctcc ttactaacaa gtatctcgaa atttctgcaa ttagacaact gtttaccatt
    45421 tgacttccta gaagcaaaaa gagcagttag agcagagtag gctgagtgat aaaaataagg
    45481 ctatgacaag ttactgccat aagccttatt ttcgagagca aaatattgag ggtgagatat
    45541 cttgataaat ggtgttccgt tagctgacat tgataataag actaaatttc aaaaaagttg
    45601 aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact aagtggcttt ttcacaacct
    45661 ctttttatct aataaccact gtttccaact caactaattt tgcccacgtt tcaatttgat
    45721 ctgttgttag atttagcgag acaacggtat gatgaccgcc acctgcctga atccatgaat
    45781 ggacaccttc atggaaattc ggtttagttt tccataacac acgtgcaaca ggtaaattag
    45841 gtgcttcagt cgttggttca aaggcttcta cttcattgat cagcagttta taatgcgtac
    45901 cgaaatctgc catcgatacg actacccctt ctcctgcttt cccatcaaat acaagacgcg
    45961 ccggatcttc tctatctccc attgataaag gagaaacaac gattcttggt ttcgtcgcag
    46021 caagcgtcgg atctacttcc atcatatggg attccaagat tgcttcttgg ccactagcta
    46081 gctcgtaagt ataatcttcc atgaatcctg tatctttccc tcgagccatg attttcagca
    46141 accgatcaat cgctgcagtt ttccagtcgc cttccccagc aaaaccgtat ccttctgcca
    46201 tcaaacgttg tacagctagt ccaggtagtt gtttcattcc atacaggtct tcaaaattag
    46261 tcgtaaaagc gttatagcct ccttcttcta agaaacggcg aataccgatt tcctgctgag
    46321 cttgtacttt tacatgttcc tcccatgtct tttcttcata atcaccatag tcgaaggtat
    46381 acaattcctt gtactcttca aataattgat cgacttcttc gtctgatacg cgatcgatta
    46441 cttgtacaag atctccgata ccaaaataat caacagtcca accaaattga atctgtgctt
    46501 ccactttgtc tccttctgtg acacccacgt ttctcatgtt gtcaccaaaa cgagccactt
    46561 ttattccaaa actttcatta taggctactg caacatccat ccaatcagca atctcttttt
    46621 ggatctcagg tcgtttccag tagccaacga cgattttatt ttgtttattt aatcgagcat
    46681 tgatgaaccc atattcgcgg tcaccatgag cggattgatt taaattcatg aaatccatat
    46741 cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc taaatgaagc aatggttttt
    46801 gtagaagctt tgtcccgcgg atccacattt ttgctggtga gaaagtatgc atccaagtga
    46861 tcacacctgc tacttcttct cgataattga cctctttcat gatttttgtg atcgtatcag
    46921 cagtaactgc taattcttga aggcggattg gataaggcaa ttggccattt tcattcagtt
    46981 catcgaccat ttcttgtgca tgtttcttta cttctcgcag tgcttcttcc ccatataaat
    47041 gctgtgatcc tacaacaaac caaaattctt tttctccaat attcaacata attagccact
    47101 ccttttattt gttttgtccg taatacgcat tttttccgtg ttttcgtaaa taatgtttat
    47161 ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag ctgtctggta aataaattca
    47221 tcttgcatac ttcatctagt acgactgcgt tcattaccgc actttgcgca tctttccccc
    47281 aagtaaatgg accgtgtccg tgtaaaagta cccctggaat tgctaaggga tcaattttgc
    47341 gttctttgaa tgtttctatg ataacttttc ctgtttcata ttcgtacccg ctgtcgattt
    47401 cctgttgtgt caaaaatcga gcacaaggta ccgatccata aaaagtatct gcatgcgtcg
    47461 tccccatcgc aggaacatcc aatcctgctt gtgcccagat cgtcgcccat gtcgagtgag
    47521 tatggacgat tcctccgatt tgaggaaatg aacgatataa aacagcatga gtaggcgtat
    47581 ccgaagaggg attgagttta ccttcaatga tatgattgtc caagtccaca acgaccatgt
    47641 cgtctgctgt catctcttca taactgactc cgctaggttt gataacgaaa tatcgtgttt
    47701 caggatcaaa agcgctcaca tttccccatg tatatttgac gagtccgtgt ttaggcaagt
    47761 caagatttgc ttggtatact tcttctttca gttgttccag cattttctat cactcctttt
    47821 tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc cctcacggta acgtctcata
    47881 aatatctgat agcctgctat ttcttctgct gaaggtgtga tggtgattcc ttggtcttgt
    47941 ccaaacacgt cttctgccaa gaagttttct aaagacttcc catgtcgatc catcatatag
    48001 gctgctaata acgcgattcc ccacgctcca ccttctccgg ctgtgtccat cactgtaact
    48061 ggtgcctcca tagcagatgc tagaatccgc tgtccaactt ctggtgtttt aaaaatcccc
    48121 ccatgcccca ctaaacgatc aatttggaca ttctcagatt tcaatatatc cattccgatt
    48181 ctcattgcac caaaagcact ggctaaatgg atccgcatga aattcgctaa atcaaaacgg
    48241 ctacctggtg tacgtacaaa taatggcctt ccttcattta ctcctgtaat gttttcaccg
    48301 gaaaaataac cataagaaag taatcccccg caatccgtat cgccttctag cgctttgttg
    48361 aataataccg tgaataattt atctctgctt atctccatac ccaaagaatt ggtaaattct
    48421 tcaaaaatac tgacccaagc attgatatcc gaagagcagt tattcgtatg aaccatgcct
    48481 actagttctc ctgaaggtgt agtgaccatg tcgatttccg gatggacatt ttctagttct
    48541 ttttctaata cgatcatagc gaaggcggaa gtaccagccg aaatgttccc tgtccctttg
    48601 gctacactgt tcgtagcgac cataccggtt cctgcatccc cttctggcgg acaaaccggg
    48661 attcctggtc tcaaatttcc cgtagggtcg atcagataag cccctgattc gcttaacttg
    48721 ccagcttctt ctcctgctac tagaacttta ggcaaaatgt gatccaagtc aatcgctacg
    48781 tgcgcttttt cgattaactg accaaaaata tttttcattt cttgatggta ggttttcgtt
    48841 gaaggatcga taggaaacat gcctgatgca tctcctacgc ctagtacttt ctcaccagta
    48901 agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat taatttcttt cacatgttct
    48961 tctttattca aaatagattg atacaaatga gcgatactcc atctctgcgg aatattgtat
    49021 tgaaattttt ccgttaatat tcgttctgct tcttcagtga ttgtatttcg ccaagtacga
    49081 aatggtacta gtaactgttc cttgctatca aatgctagat aaccgtgcat cattgcactg
    49141 aacccaatcg acccgattgt cgtaagattc tcgccataga tttcgaatac ttctgctgcc
    49201 atttttcggt aactgacgcg cagtcctttc caaatatctt ctaatgaata ggtccagata
    49261 ccattttcta gtttgttttc ccactcataa ctaccagcgg caataggagt aaaagatgaa
    49321 tcaatcaata cagctttgat cctagttgat ccaaactcaa tccctaaaga tgtacgacca
    49381 tctatgacat ctaacatact ttgcgcattt acttccttca taatgattta actccctcct
    49441 tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt acaatgatta ttgtatactt
    49501 ttgtacgtac atgtcaagca gttatttaca agaatacaat aaaaaacact tgttttgtac
    49561 gtacataagt tataaaatga aattgcttac aaaatctgat caaccgcctt ctcgaaattc
    49621 tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt cctcatcgtt tatttatttt
    49681 tttggcgcat ttggcggcat tctatttgga tatgatattg gtgttatgac cggcgcctta
    49741 ccttttttac agcatgattg gggtttggca ggtaaagctt ctttgatagg atggatcacg
    49801 tcttcggtca tgttaggtgc gatccttggc ggctctttat ctggtcttct ttctgataag
    49861 cttggacgac gaaaaatgat tcttctttct gcacttattt ttatggctgg ttctgtcctt
    49921 tctgcatctg cccctcacaa tggttcttat tttctgattg cagcaaggat tcttctaggt
    49981 ttagctgttg gcgctgcatc tgccctagtt cctgcttata tgtctgaaat ggctcctgct
    50041 agactaaggg gaagattgtc agggatcaat caagtgatga ttgcttctgg catgctcctt
    50101 tcctatgttg cagattattt gctaaaaggg ttgcctgaaa cgatggcttg gcgcgtgatg
    50161 ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg gtgttttagc tctaccagag
    50221 tcaccgcgtt ttcttatgca atctggccgc ttggaagagg caaaaagagt attgaactat
    50281 attcggacac caaaagaagc tgaacaggaa ttcgaacaaa tccagctgaa tgtcaaacaa
    50341 gaaaagacaa ccggaacttc ttggcatact ttgtttttag aaaaatatcg ttccttagtc
    50401 attgccggta ttggtgtagc agtattccag caatttcaag gagctaatgc tattttttac
    50461 tatattccgc tgatcgtaga aaaagcgact gggcatgcag cgagcgatgc attgatgtgg
    50521 ccgatcatcc aaggaatcat tttagttgct ggttctttgc tctttttggt tattgcagac
    50581 aagttcaatc ggcgaacttt attgaaaata ggcggtagcg tgatgggact ttctttcatc
    50641 ctaccagctg ttttaggaac tgtactggat gctcatacaa attcgttgtt gattctcttg
    50701 tttttatgta tttatgtcgc attttattct tgcacatggg ctccactgac atgggtaatc
    50761 gtaggcgaaa tattcccact agctgtccgc gggcgagcat ctggcctagc ctcttctttt
    50821 aattggattg gttctttcct agttggcttg ctttttcctg tgatgactgc ttctatgtct
    50881 caagaaatcg ttttcggcat ttttgggatc atctgctttt taggggtttt atttattcaa
    50941 gagatcgtac cagaaactcg tgggaaatct ttagaggaaa tcgaacaaag tgcatctaaa
    51001 aaaacttatc ctaaacgtat ttctatccat cattcttaaa gaggttgtga cagaagtggt
    51061 cagcttcaag aaataagaag gaaccgctta gtttccagtc acaaggaaca atttcaaaaa
    51121 acagcttctc atgtttgatg aatattgtga cttgtgacca gttggtagga gaacaccgtt
    51181 tagtggagag gtggtgaaag aaacgttcag ccttgagcga tgacaaaagc tctcacaaac
    51241 attgcgacga gtaaccgtag gagcaataag aggcggtaaa aacccgtctc ttatttttcg
    51301 ttttctttca ctgtttcccg ttcaactagt acaggttcat agagaatatt tggctgtgcc
    51361 tgtccttttt caatcgttgt gacgatccac cgtgcggcat cttgtcccaa cttttctttt
    51421 ggatgcgtca atgttgtcaa ttttttattc cctaaatggc ttaaagaaga atcgtctgtt
    51481 cctacgatcg ataggtcatc tggaatatga tagccatttt ctgtcaactt atctagcatt
    51541 aaactagcta tttgatcgtt atagcaaatg atgccatcta aagcttgttc ttctttcaac
    51601 cgttgtactg ttttttcagc gattgtttct cgtgtttccg tggtgaaagt aatgatatct
    51661 tccgtcaaaa aacgcacatt atactgttca cacgcgctga taaaaccttt catcctgtat
    51721 tttccttgaa gatcgtcgat ttttgtgatg aacaacagac gctgatgtcc tttttttatc
    51781 aaatattctg ctgccataaa accgactttc acatcatcta aacaaatata agaggcattt
    51841 aactcttcat aagcagcatt gatcatcacc atcggaatgc cttgttctct caaagcaaca
    51901 taaagtgcta agtttgggtt atactgatta cttttcgtag gctcaacgat caaaccatct
    51961 accccataag caatcatttt cttcaaacac tctttctcca attcgtgatc gttattcgta
    52021 ctggctaaaa gcaatgaata cccagattct cgtaattcat tttcgattcc tcggataatc
    52081 gacggaaaaa tataatcaga gagatatgtg gtgatgacac cgatcgtttt tgttctgttc
    52141 aatggtgttt tttctttctt atagctgtca tctacatatg tgcccgaccc tttttcttta
    52201 cgcagatagc cctcatttac caacacagcg attgcttgcc gaatcgtatg tctgctgacg
    52261 tcatatattt cttgcagctt gaattctgag ggaatggttg tatttacttc gtattcctca
    52321 cgaaggatct tttgttttat ctcttctgca atatcttgat attttgccat ttgccttgcc
    52381 cctttttatt tgtacgtaca tatatcataa cgagtctgca aggtttattc aagcaaaaat
    52441 caaggtttct tgtgctcata aggcatacgg aacaggttca ccaaattctg gaaatccttg
    52501 ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta ggattgtcta acggatcccc
    52561 tttttggttc ttctctggtc gagcatgata gatcaaaata tcaaagcacc cgtcttctga
    52621 aaccgtaaac gagttgtgac caggaccaaa ttgcttattt ttttcagaag aaacgaatac
    52681 tggctccgat aatttatgcc atgattcttt taataaaaga ttcttgtgct catcagccca
    52741 cagcaggccc atggcatagt tttcatctgt tgcactacca gaatacgtaa tgaatatttt
    52801 cccattgcga gtaaggactg ctggaccttc atttactgaa aaaccccgtt tttcccattc
    52861 atactcaggt atagaaagga ggatctgctt cccctttaac gtccaaggat tatccatttc
    52921 ggatagataa agattagaat tcccagtaat ttcaggatct tgttgggccc acacataata
    52981 tagctttcct tgagaagaga aagttgttcc gtctaaactg aaagtttccc ttttcgtttt
    53041 tatttgtccc ttttctatcc aatctgtatt cattggatct atatgctgat tttcaagtac
    53101 gaacatgcgg tgatgatgag attcatcacg tatagtttta tgattgcttg cagcaaagta
    53161 aatataccat tttccattga tataatggac ttcaggtgcc cagatcaatt cactcatcgg
    53221 tccttcttca tgagcctgcc atacaatcag gcttgcagca tgttctaatt cattcagtga
    53281 ctttgccctt cttaattcaa tcacttggta gcctggaacc gatcctgtaa aataataata
    53341 accatctgta tgtttatata cccaaggatc tgcccgttgc cgaacgatag gattattata
    53401 aagtgtcttc atcataacaa atttcctcac attttcttta ttctaattac attccaagag
    53461 tatttttcta tccctacatt taattcgtca tgtttttcta ttgaaaaata gtttatttca
    53521 ggttttattt tttctggttc ttcacgtgta tttttgtcca acagatcgct actggttaat
    53581 gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt caatcgttat cttttgttgt
    53641 tcatttgtgc gattgatcaa gaaaatagtc agctgattgc cgttatctac gatcacactg
    53701 tccacataag gaacattttt tatcttttca caatcataaa cagggcaaat gatttgttgt
    53761 ttcaatactt gacctttagc atatttcgat aagtgttgca tcacataata agttggattc
    53821 ctccatgctt gtcctttagc atctgtcagt attaaaggta ttgtgtttac taacaatgat
    53881 tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag ccagccctaa tgaaccaaat
    53941 aataatgtat cttccatagt aaagtaacac caatcgatgg gactaccgac ttcaaaatcc
    54001 ctatgtcttt tttccggttg ggcatgtaca ttccattcat caaacgctag atacattgtt
    54061 ttctcacttc tcaaatatcc ttttacataa tcacaagtag caacgatttc ttcaatttga
    54121 cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat catcacgctc atttttagga
    54181 ggtttagtca agtcttcttc atcatactta tcaatatagt tatgcaaagc taaataatct
    54241 acattctcat aagcttcttc taatgcgata cgatcccatt cagggtacga tggtaaacgt
    54301 ggcgtagagc ttccaactaa gattgtttca attgaatcat ccactaactt catgactttt
    54361 gaggcttcat ttgctaacct accatattca taagcactct tagcccccac ttgccatgga
    54421 ccgtctagct cattccctaa acaccataat ttaacgccaa aaggcttttt ttgcccattt
    54481 tttattctta aatcactcca gaaggttcct tttggaaaat tacaatattc tactaaattt
    54541 tttgcttcat caattccttt tgttcctaaa ttcaccgtga atatcggttt cgcttgtaca
    54601 tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat ttggttctat acctcgccat
    54661 gctaaatcaa ttttttttgg tcgttgttct ttcctaccta cagtatccac ccaatcataa
    54721 ccagatgtaa agtttcctcc aggatagcga atgacaccta attctaattc ctttacataa
    54781 gagaggacat cttgtctaaa cccgtcacca tcagctgttt catggcccgg ttcataaatc
    54841 cctgtataga cgacacttcc catatgctca acaaatgagc caaacagccg agaactgatc
    54901 tctcctaccg taaaatcctc tctgattttt actttcactt cttcgtccaa cagctttccc
    54961 tacctttcat ttcctatagt caagataata gatacaagtt tccatacgcc accagcaaag
    55021 ataaaaataa aaggagggaa aagaaatatg agcagtacaa atcctaataa caatacgcaa
    55081 caaagaatat ttctcaaaaa atcaaaaaat actttccatg cactttgttc aaaaatttgt
    55141 aaaaatgtga tatctttgtt ttcagaaatc acatatatcc aggataaaag aaacatggaa
    55201 taaataagta aaactacata tccactaatt gaaagtacga ttgtctgttc ttctaataat
    55261 tgattaacaa gaaaataaat aatgatcgtt aacagactaa ggaacaagtt cttaccaaaa
    55321 tgttgcttta ttccttgaaa gaaaaaataa atgatatttc ctgatccctc acctttccat
    55381 tgcgccatta cataaaaaac agcacatgta gcaggcacga tagtaataac aggcaaacac
    55441 acgattaccc agagaatacc taataagatg taatcagaaa ccacaatcaa ctgtccataa
    55501 agtttagtct caactatttt catcttcctc actctttcat tccagacata gctgttcctt
    55561 gaacaaaata tttctgtgca aaaatataaa ccactagtaa tggcagcatt gaaataaccg
    55621 ttccagccat tacaggtcca taaaaagaaa tgttctgccc tgtaaataaa gctaaccctg
    55681 ctggtaaagt tctcattttt gaactacttg tcaatatcaa tggataaagc aagtcattcc
    55741 aactattcat cagcgtgaaa atccctaatg ataaaagagt aggtttgctc aatggcaaca
    55801 tgattttcaa aaaaatatta aactcactta atccatcaat gcgtgctgcc tcttccaaag
    55861 aatctggtaa agtaataaaa aaggaacgca tcatgaagat accaaatgca gttgtcatac
    55921 gaggaatgat caaacctgca taagtatcaa gcatgccaag caaattgact tcaataaata
    55981 gaggaatcat gaagacttgg aatgggatca tcattgtcag caaaacgaaa tagaacaaaa
    56041 ctgattttcc tttgaaacgc atacgagcaa atgcataccc cgcaagagaa tcaaagaata
    56101 cagaagtgat cactgtccct ccagcaaaaa tcactgtatt tttgatgtaa tctaataaag
    56161 ggatcgttgt ccaaacctta atatagttat ctagggtata gctatctgca aatattctag
    56221 gcggaaatga gataatgtct ttttcgtgtt taaaagaaga aataatcagc cacaaaaaag
    56281 gaaacgcaat aaccaacaat ataactagca tactaatcgt tcgaatgatt tttttaccac
    56341 taactgcttt tcgactattc acagtaactg taatcgagct ttctggtgcc atacaggttt
    56401 ccctcctatt tcatttgttt ttctttccgt gacatgtaca cattagtgga gatagctata
    56461 gttgcaacaa tgataaacaa aaccgaagac agtgctgatg catagcccaa ttcataaggt
    56521 gctgtaaatc ctcgactgta aatatattga acagctgttt ctgtcttaaa ttgtgggcca
    56581 ccagcggttg caacatatac ttggtcaaac acttgcatcg aaccaatcag attcgttaaa
    56641 atacaaaaac ctaaggaggg aacgatctga ggtagcgtaa tgttaaaaaa ctgctgtcga
    56701 tttgttgcac catccatttg agcagattca tacaaagagg gagagattcc ctgaatagca
    56761 gtcaagagaa tcgtcatagt aacaccaaaa ttcttccaaa cagtcatgat ggctaccgtc
    56821 ggcatagcaa gggttggatc gcgaaaaaat tgagggatcg gcaagccgac ttgtgaaaac
    56881 aagtaaggaa tcgcccctac attcggatca agcaacatag aaaaaataat cgcaattgac
    56941 gtcaatgaac aaataactgg aatgtaaaat gtcgaacgac aaaaacgatt gaatctggta
    57001 tttttagata atgctactgc tgctaataaa ccaacgataa tttgtgttgg cgtttcgagt
    57061 acggtgaaat ataaagaatg aatcaacgaa ttaactgctc gttcatcact aaatatacgt
    57121 gtgaaatttt caaaacttac aaaatttctt gatgtaaaga aaatattcaa atctgtaaaa
    57181 ctaatagcaa atgttcctaa aagcggtatg acagtaaata ataataaaac taaaaaagat
    57241 ggagttaaaa acaaatacgc agcacgatca ggacgattcc aatatttggt gaatccgttc
    57301 atatagtcca cctcatcttt cattttgata aaatatacaa tcttggttta aacgaatctg
    57361 aataagatat ccgaacaatc gctgtgattt ttgcaataac acctatctgc aaatactagg
    57421 tcgatcattc agatatctgt atcttgccaa cagtctatcc tgtataaagt tctgtactat
    57481 ttttgcagta attgattgat ggcatcatct gcatcgttca tgagtgtctc aggatcttct
    57541 cctgctaata atttttcaat cagtggattg atgatatcgt tattgatcgc agagatttgg
    57601 gtaaaacctg gtaaatatgg ctctgcatag tccatttgtt tcgataattc agatacaata
    57661 ggatcatttt ttacttcttc atcttttgct actgaatcca agtaggcagg aaaaccattt
    57721 tcgatactcc attttttacc aatttcagtt gtattccaat atttcaaaaa ctcatagaca
    57781 gcttcttttt tattattatc tgtacttgcc ggaatggcat atcctacgcc atctaaaata
    57841 gctgtttttt tgtctgaatc actcccttgt ggtaaggtag tcacattata atcaatctcg
    57901 tttgctttca atccgttatt taaccatggc ccattgattt caatggctag ttgaccagca
    57961 ttcattaaat tgtccatttc ggcgccagaa atcgattctg gaccagtctt gtctacatgg
    58021 atcatttttt ggatcgcttt taacgtttta aggttctcag gagacgcaaa ttcagactta
    58081 gtataatcat cgttcactaa tttaccgcca tttgctaaca tccagttata taacggtgca
    58141 gttccatcct tattgaagac aaaacctgat acatttttac tactatcagt taattttggt
    58201 gccatttctg tcaattcttc ccaagtagta ggtggttgtt cagtatctaa tccagcttgt
    58261 ttaaacaaat cattattcca atacatatac atgccttgta cctgcatggg gacaaaatac
    58321 tgtttacctt ctacttcacc taattcaata gccgtttctt taaaatcaga tttatctact
    58381 ccatcatatt cccagaaatc gtctaatggt tgtaccgctc cgttttctac atactgtgca
    58441 aaatctggat aatttaatgc aataaagtca ggtgcttttt tagaagaaat cgctgtagga
    58501 agtttttcat tcaaattggc ccaagtcatg atatccattt caattttgat gtttttatca
    58561 tttgttttat taaattcatt aacgatttct tgcaatattt caccatcaga tgcagtaaac
    58621 ccattccaaa aagtcaaagt cacttcttta tcagaatcag attctgctga acttccgcca
    58681 ccgcaagctc ctaaaatacc tgttgcagct accaacaaag agccaattaa ccatttttta
    58741 cctttcattt tttgttcctc cttcatctga taaacgacat ctgaccacat tccaagatag
    58801 cggtttcaat aaacaattga ttttcccatc ttcagctttt acatttgttg aatgacttgg
    58861 ttttacttgt tcacttttag gaagattagt tgcttttgta tcaaatccac tcatttcaga
    58921 aaaatcaatc actccctcta atgataattc agacaagtga acagttagat tcatttcttc
    58981 ttctccacgg ttaactgcaa atatgacaag ttctttgtca tttattacgg ctatactttc
    59041 taaatacggc acttcttcaa aatcttccgt cgcatagttt gctgaacgaa tttgtgggct
    59101 taacacctct ccttgtccat acacagcagc ttgcataaat ggataataga tcgtctgtct
    59161 ccaagcttct ccgtttgttt ctgtcctgat tggtgcaata acatttacca attgagcaag
    59221 acaagcaatc tttactctat ctgcatgttt caataaagtg attaatagac agcctaatag
    59281 taatgcatct tcaaaattat agttatcttc tagtaaagga ggagcaatct gccaaggcgc
    59341 tgcttgctca tcttgttccg ctgaatgata ccaaatattc cattcatcaa aggacaggtt
    59401 gatctgcttc ttactatgtt tcttcgcctt gactgtatcg cacatcgctg tcacttcaga
    59461 aatgaaccga tccatgtcta aggaacgtgc aagataatga gaaagatcat ttttttggtt
    59521 gccataatat tgatgcagag acaaataatc tatttgatcg tacgcttgat caagaactgt
    59581 taactcccaa tcaccaaatg taggcatttg ccgattcgaa cttccgcata aaaccacctc
    59641 aattgaatca tctacgagtt tcatcgcctt agccgtttcc tctgctaacc gtccatattc
    59701 ttccgctgtt ttatgtccaa tctgccaagg accgtccatt tcattcccta aacaccatgt
    59761 cttaattcca aaagggctct cttgaccatt cgaacgcctt aaatcactcc aataagttcc
    59821 tttaggaaaa ttacaatatt cgactaaatt acgagctgca tctactccac gagtacccag
    59881 attgacagcc atattgattt cggcccccac atgctgacac cagttataaa attcatgaat
    59941 cccaacttca ttcgtttcaa ttgtccgcca agctaaatcc aaccttctag gtctatttgc
    60001 tatgggccca atactgtctt cccaacgaaa tcctgacaca aaatttccac caggatatct
    60061 aatcatggga atgttcaatt cttttaccaa gtgaatgacg tcttggcgaa atccactttt
    60121 attagctgat ggatgatctg gctgataaat cccttcatat actgctcgtc ccatatgttc
    60181 aataaatgaa ctgtacaacc gcggatcaac tttgcctaca cgcttgacac tactaatcaa
    60241 cattgtggct tccataactg acctccttta atttatacat gaataataca cctcgttgaa
    60301 agcgttgtca ataatataaa tgttaaactt acttattttt tatcgatttt aattaaccaa
    60361 aatattaata atgattaaaa caaataattt aatataactt ttattaacaa atatgttaat
    60421 atataagaaa aaccttttaa gaggtgatat atatgcaact acaaataaac gaagaatcct
    60481 taccggtata tgaagcatta gctagtaaaa caaggatcaa gatcatccag ctactttcca
    60541 aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg agtgagcagt gctatcacga
    60601 cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa aacagaaaaa gtaggacaac
    60661 aaaaaatttc gagccttcgt gtcgataaaa tcgatatcag ttttccagaa aaaatattca
    60721 atgcttttga tacgaaagaa acatcgatac ctattggtca ctatacaaat tatgcaattg
    60781 aaccaacatg cggtttagct acgatccatg attttatcgg gaaagtggat gaaccacgct
    60841 attttatgga tcctcgaagg atggatgccc gcattctctg gtttaccagc ggatttgtcg
    60901 agtatcaggc acctaatttt ttaaatacag aggatacatt ggagatgttg gaagtttctg
    60961 tggaaatttc ttctgaattt cctttttcaa atgacaattg gccttcagat atcactttca
    61021 gcctaaatgg tgtggagcta ggaacatgga cgagccctgg agattttgct gatatccgcg
    61081 gtaaatatac gcctgattgg tatccagata atcttaatca atacggcttg ctgaagacga
    61141 tccgaattac caaacacttg acgaatatga atggagaacc cttgtcaaac atcacgatca
    61201 acgacatccc taaagagcaa gacacttggc atttgcgaat cgaagtgaaa gacgatgcca
    61261 aacatgtggg gggctgtact ttatttggta aaggattcgg taattacgat caggatatca
    61321 aattaaaagt ttactacagc tgatattttt cttttctatt ttataaaaca aaaacagcca
    61381 atccctatga agggacggct gtttttgctt tattgttatt tccagaaatc atccaaaacg
    61441 gaaattggca aatgtcgttt atgttgtgtc ttgttccacc atgcttcgat tttttcttgt
    61501 gcttgagcgg gaacttcttt tccttctaga taatcatcaa tttcattata tgtgacacct
    61561 agtgcgactt catctgcaat cattggtttc ccatcttcaa gatctgcagt cggaattttt
    61621 gtgtataatt tttccggtgc attcaatgct tgaagcaatt gtttcccttg tcgtttgtcc
    61681 aaacggaata atggcaaaat gtcggctcca ccatcaccaa atttcgtgaa gaatccagta
    61741 atgttttctg cagcatgatc tgtccctaat acggctcctg ctctttctcc agctacggca
    61801 tattgcgtaa tcatacgttg acgtgctttg atatttcctt tgttaaaatc gctgattccc
    61861 acacctgctt cgcttaatac ttgtacttgt gcatcaacag ctggtttgat atttactcgc
    61921 agactaacat cgggctggat aaagtcaaga gcagcttttg catcttcttc atctgcttgt
    61981 tcaccataag gaagacgaac agcgataaat tgatagtttt catcttttgt ttcttcacgc
    62041 atttcttcca tcgttagttg cgccaaacgg cctgctaaag tagaatcttg gccgccgctg
    62101 atacctagta caaaagtttt aagaaacgga tattttttca aatatgcttt cataaagtca
    62161 atacttttac gtatttcttc ttttggatca attgttggtt tgacacctaa ttcattgata
    62221 atttctgtct gcaggctcat ttattctcct cctatgcttc tgtatgaagt tctgctacgc
    62281 tttttttcat tcgttccatg atagccattt tatggttcca gcattcagta gacagatcga
    62341 ctgggtattt ttgcggattc aagtctcgtt tatactcttc ccatagtgaa tctagatttt
    62401 cttttgtata ttctttgatt tcgtttagtg caggtaattc gtaaactctt tttccttcga
    62461 taaagatatc ttgtaaaact gggcgagctg taaagtctcg aacagtttta ttgataaacg
    62521 tatgcactgg atggaacatg aagatagctt cttcttctcg agggtcttca tcccataatg
    62581 tcacgtagtc tccttcagat ttcccatcaa aatttcgagt gatgcgccac acttgttttt
    62641 tccctggagt agttactttt tctgcattgc ttgataactt gatcgtatcc atcatttttc
    62701 cttcatcatc ttcgatcgat actagcttga atactgcacc aagggctggc tgatcatagg
    62761 ctgtgatcaa ttttgtccca acaccccata catcaatttt agctttttgc atctttaaat
    62821 tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat ttttgcttcc gtaaatccgg
    62881 ctgcatctaa ttgttcccga acacgcttag atatataggc catatctcca ctgtcgatgc
    62941 gtacgccttg gaagttgatt ttgtcaccta attctttcgc cacgcgaatc gcacttggca
    63001 cacctgattt caacgtatca taagtgtcga ctaagaacac gcagtcttta tgggttttag
    63061 cgtaggccat aaatgcttca taatcatttc cataagattg gactaacgaa tgagcatggg
    63121 tcccgctagc tggtatccca aatatcttcc cagcacgtac attgcttgtt gcatctgctc
    63181 cgccgatata tgccgctcta gttccccaga ctgctgcatc cagttcttgt gcccgtctag
    63241 ttccaaattc caatagtgga tcttctccga tgacagattt gatacgagct gcttttgtcg
    63301 cgatcaacgt ttggaaattg accatattca acagtgctgt ttctactaat tggcagtgcg
    63361 ctaaaggccc ttccacttgg attaatggtt catttgcaaa aactagttct ccttcacgag
    63421 cagaacgaac tgttgcttta aattcaaaat tttgtaaata ctccaaaaaa ccttcaggat
    63481 agacttctaa tccacgcaag taagcaatat cggaatcatt gaacgtcaga ttctctaaat
    63541 aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc gtggttaaaa ggcatttctc
    63601 gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc taattcccag tatgtctgca
    63661 tcatattgat ttgatataga tccgtatgca gcgtcaaact gtcgtctggg taagtattat
    63721 tcatcttgta ttactcctct tggcaagttt tcatttccta cccattataa caaaaaaaat
    63781 taataggaaa agaaatcttc cattttttct gaaaaagcac atgttttacg gatatttctt
    63841 gctaaaaatg aactccatct gctaaaacta tcctttctta ttggtattta acctaaacga
    63901 gaaagaatac ttattttatt caatgtatcc gagacacgaa gaaattagcg ttttctattt
    63961 ttcttacttg ataaaataag tagattttcc tactacaatc gtagccgtta agataccgat
    64021 gattgaacaa atataaccaa atgtctctac aacataatca atggcaaaag tttttgaagc
    64081 aataatacgt ctttcctttt ttgcttctta tccccaaaga aacggatata acttattatg
    64141 tattgaattt ttttggaaag gcgaattatg acaagatata ccttgcgttg aaaaaactcg
    64201 cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa gaaagcagct gtcaatagaa
    64261 tggaaacaaa aatggcgaga catcaatcag tctcgccttg ttttttattg tatacattag
    64321 atatggagca ggttaccata aagatgatta ttagtcgtta ttaaatagaa tccttcgtat
    64381 ttttttgttc tatcactaca atatctttat ttggacgttt gaattgtatt ccggcacgtt
    64441 ctagctcctg tttcactcct acatggactt tgacatcttc tcgtgtcatt tttaaaacgt
    64501 gatgaatcgc atagtcgatt tcttgccttg tgattttttt cttcgtattg atcatcacga
    64561 cttgcttttt atcatatgtg tctaccaata gcagttgtgt tcgatctaac gaaaataatt
    64621 tgtaataatt aatagccgta ttcccaaaaa agaattccac tactttagga taaaatgtct
    64681 taatatttaa attactattc acaggtgttt cgattagctt catctcattc ctcttttcta
    64741 tatagtcgat ttagatccaa tcttaagtta tgttaccatc tttcctagca ttaaggaatc
    64801 ggttacaatt gaacctttaa agacaaaagg atttaccttt tatcacttaa tagtataaca
    64861 taatttcgtg agaaaaaaat acaatttttt cttgaccatc tcgttacgta aaggattatg
    64921 cttacatttg tcaggaggaa aaataatgga gtacacaata aagaaaatgt cagagatatc
    64981 aggagtcagt ccgagaacct tgcgctttta tgatgagatt ggactgctaa aaccagctag
    65041 gatcaattct tcaggttatc ggatttatgg caaaaaagaa gtcgatcgtc tgcaacacat
    65101 cttattttat cgaacaatgg cattcaagtt ggatgatatc caagaggtct tagacaatcc
    65161 atcttttgac caccagaaag ctttaatcaa gcatcagcaa atgttgctgg aaaaacgggc
    65221 acaaatcgat accctcttga caactgtcca acagacactt gatatgtacg aaggaggaag
    65281 aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa caaaagttga aagaaaatga
    65341 agaatcctat ggacaagaga ttcgacaaaa atacggaaaa aaaaaggtta cagcatccaa
    65401 tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa atgcaaactg ttgaaaagca
    65461 gatgttattt gatctcactc tttacttaaa agaaccagaa gatgaaagct tagcagaccg
    65521 tatcttccaa gctcataaga agtggttctc ttacagctgg cctgaatatc agccaaaagc
    65581 gcataaagga ctaggaatga tgtacgaagc cgatgaacgg ttcactgcat attatgacga
    65641 gcgaagtggt catggtgcag caaaagcttt aaatgaaatt atccaaaaaa aggcgtaatc
    65701 ttttacatga tccaacaaaa aagtacacgt aaaatgacct aataacaaaa cagcagacac
    65761 ccaagttaga tttttatgct aacttttggt gtctgctcca ttctttcaca agttttttca
    65821 tcctacttta aatatattca tcgttgaata aacgtataag gtattttcac tttatccttt
    65881 ttttctgtaa gaaacaattg aaatgccatt tctccgcaag cttccagatg gtggtcgatg
    65941 gttgggaaac caagaagctc gctgatcagc aaattatcgc gtccaatcac cactggtgtc
    66001 ttatcttgca gatagctttg caagatgcct gctgcaacca tatccgaatt tgtaaagaca
    66061 ccatcaactc caagctcttt aaaaaagaag cctgcttgtt ttcctttctc tgcatctata
    66121 caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa gttgatgaca tagctgcaaa
    66181 gtgatttttg agttataact tagcctgcca cttcgcccca acgtgatgcc caattgcttt
    66241 ataccttgct gcttcaaata agtaagacct tctgtaattg attgttctcg atcaataaag
    66301 acacagcttg cctgttttcc cttgatttct tcacaaaaaa ctagaggtcc atatttttga
    66361 tatgccaata aatcatctag tgtattggca cgagacgtaa tgatcaatcc atcgaaagct
    66421 ttagctgcaa attcttcaag ataccttcgc tctttttctt tatgatagcc cgtcggtaaa
    66481 agtgtcactt tgtaatcata atcaaacgct gcttcaatga tcccactgat caactgatca
    66541 aaatacggat gattggtata aggcagaatc actccaatgc ttttcgtttt tccataactt
    66601 aactgtctgg catttgcatt agggatataa tcgacttctt tcatgaccgc aagtatttct
    66661 ttcctttttt cctcatctac atatggatgt ccattgatta cacgtgaaat agtagaaact
    66721 gaataacctg tgagctttgc gatatctcga atgtttgcca ctgctgcttc gccacctata
    66781 caaaaagatt ttcttgtaaa tagtctacca cagctggacc gcattcaaaa aactgataca
    66841 tttttccttc atgcgaaaaa ctgatccgct gcccgcccca atatatatct tttgtcaaat
    66901 tttcgatttc attcattggt atttctttta ctatattcct attcaaccct tgaaaactgt
    66961 acaacgttaa tacggatccc ttgaactcga caaacgcata aaaataattg ttcgttgtgc
    67021 catctgttat ctctacataa gtattcattg caagccctcc ttttgatact ttgagtatag
    67081 tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca aagaaaagtt ttattcctct
    67141 ttcattatta ccaaagtctc ttattttaaa ttgaatacat tataatgata ctgtaaatat
    67201 atctttatta ttagtgaat
//
LOCUS       DACDRK010000008        62320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDRK010000008 DACDRK010000000
VERSION     DACDRK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.008"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWJ77_000609"
     CDS             591..1280
                     /locus_tag="IWJ77_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9452256.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWJ77_000610"
     CDS             1267..2475
                     /locus_tag="IWJ77_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9452257.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWJ77_000611"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWJ77_000611"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9452258.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWJ77_000612"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWJ77_000612"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9452259.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWJ77_000613"
     CDS             complement(6578..7273)
                     /locus_tag="IWJ77_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9452260.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWJ77_000614"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWJ77_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9452261.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWJ77_000615"
     CDS             complement(8472..8663)
                     /locus_tag="IWJ77_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9452262.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWJ77_000616"
     CDS             complement(8684..9574)
                     /locus_tag="IWJ77_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9452263.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWJ77_000617"
     CDS             complement(9561..10328)
                     /locus_tag="IWJ77_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9452264.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWJ77_000618"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWJ77_000618"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9452265.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWJ77_000619"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWJ77_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9452266.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWJ77_000620"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWJ77_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9452267.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWJ77_000621"
     CDS             14944..15282
                     /locus_tag="IWJ77_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452268.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWJ77_000622"
     CDS             complement(15450..16208)
                     /locus_tag="IWJ77_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9452269.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWJ77_000623"
     CDS             complement(16226..17044)
                     /locus_tag="IWJ77_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9452270.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWJ77_000624"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWJ77_000624"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9452271.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWJ77_000625"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWJ77_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9452272.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWJ77_000626"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWJ77_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9452273.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWJ77_000627"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWJ77_000627"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9452274.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWJ77_000628"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWJ77_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9452275.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWJ77_000629"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWJ77_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9452276.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWJ77_000630"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWJ77_000630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9452277.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWJ77_000631"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWJ77_000631"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9452278.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWJ77_000632"
     CDS             27086..27385
                     /locus_tag="IWJ77_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9452279.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWJ77_000633"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWJ77_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9452280.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWJ77_000634"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWJ77_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9452281.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IWJ77_000635"
     CDS             28352..30325
                     /locus_tag="IWJ77_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9452282.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IWJ77_000636"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IWJ77_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9452283.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IWJ77_000637"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IWJ77_000637"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9452284.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IWJ77_000638"
     CDS             33204..34496
                     /locus_tag="IWJ77_000638"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9452285.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="IWJ77_000639"
     CDS             35232..36095
                     /locus_tag="IWJ77_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9452286.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="IWJ77_000640"
     CDS             complement(36224..37846)
                     /locus_tag="IWJ77_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9452287.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="IWJ77_000641"
     CDS             38095..38616
                     /locus_tag="IWJ77_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452288.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IWJ77_000642"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IWJ77_000642"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9452289.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="IWJ77_000643"
     CDS             40697..41515
                     /locus_tag="IWJ77_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9452290.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="IWJ77_000644"
     CDS             complement(41618..41815)
                     /locus_tag="IWJ77_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452291.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="IWJ77_000645"
     CDS             41984..42562
                     /locus_tag="IWJ77_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9452292.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43607..44110)
                     /locus_tag="IWJ77_000646"
     CDS             complement(43607..44110)
                     /locus_tag="IWJ77_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9452293.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44469..45494
                     /locus_tag="IWJ77_000647"
     CDS             44469..45494
                     /locus_tag="IWJ77_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9452294.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45817..46695
                     /locus_tag="IWJ77_000648"
     CDS             45817..46695
                     /locus_tag="IWJ77_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452295.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47171..47287
                     /locus_tag="IWJ77_000649"
     CDS             47171..47287
                     /locus_tag="IWJ77_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9452296.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48215..48553
                     /locus_tag="IWJ77_000650"
     CDS             48215..48553
                     /locus_tag="IWJ77_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452297.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48647..48877
                     /locus_tag="IWJ77_000651"
     CDS             48647..48877
                     /locus_tag="IWJ77_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452298.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49277..49648
                     /locus_tag="IWJ77_000652"
     CDS             49277..49648
                     /locus_tag="IWJ77_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9452299.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49922..50614)
                     /locus_tag="IWJ77_000653"
     CDS             complement(49922..50614)
                     /locus_tag="IWJ77_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9452300.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50617..51402)
                     /locus_tag="IWJ77_000654"
     CDS             complement(50617..51402)
                     /locus_tag="IWJ77_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9452301.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51639..53039
                     /gene="lacG"
                     /locus_tag="IWJ77_000655"
     CDS             51639..53039
                     /gene="lacG"
                     /locus_tag="IWJ77_000655"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9452302.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53064..53381
                     /locus_tag="IWJ77_000656"
     CDS             53064..53381
                     /locus_tag="IWJ77_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9452303.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53394..55067
                     /locus_tag="IWJ77_000657"
     CDS             53394..55067
                     /locus_tag="IWJ77_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9452304.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55081..56712
                     /locus_tag="IWJ77_000658"
     CDS             55081..56712
                     /locus_tag="IWJ77_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9452305.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57584..58774
                     /locus_tag="IWJ77_000659"
     CDS             57584..58774
                     /locus_tag="IWJ77_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9452306.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            58908..61097
                     /locus_tag="IWJ77_000660"
     CDS             58908..61097
                     /locus_tag="IWJ77_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9452307.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            61094..>62320
                     /locus_tag="IWJ77_000661"
     CDS             61094..>62320
                     /locus_tag="IWJ77_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9452308.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT        19424 a        11323 c        12309 g        19264 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
    43321 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
    43381 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
    43441 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
    43501 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
    43561 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
    43621 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
    43681 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
    43741 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
    43801 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
    43861 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
    43921 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
    43981 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
    44041 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
    44101 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
    44161 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
    44221 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
    44281 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
    44341 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
    44401 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
    44461 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
    44521 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
    44581 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
    44641 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
    44701 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
    44761 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
    44821 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
    44881 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
    44941 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
    45001 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
    45061 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
    45121 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
    45181 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
    45241 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
    45301 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
    45361 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
    45421 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
    45481 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
    45541 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
    45601 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
    45661 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
    45721 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
    45781 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
    45841 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
    45901 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
    45961 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
    46021 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
    46081 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
    46141 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
    46201 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
    46261 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
    46321 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
    46381 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
    46441 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
    46501 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
    46561 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
    46621 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
    46681 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
    46741 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    46801 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
    46861 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
    46921 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
    46981 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
    47041 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
    47101 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
    47161 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
    47221 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
    47281 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
    47341 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
    47401 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
    47461 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
    47521 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
    47581 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
    47641 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
    47701 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
    47761 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
    47821 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
    47881 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
    47941 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
    48001 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
    48061 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
    48121 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
    48181 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
    48241 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
    48301 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
    48361 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
    48421 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
    48481 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
    48541 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
    48601 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
    48661 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
    48721 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
    48781 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
    48841 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
    48901 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa
    48961 tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct
    49021 acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta
    49081 gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa
    49141 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
    49201 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
    49261 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
    49321 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
    49381 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
    49441 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
    49501 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
    49561 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
    49621 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
    49681 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
    49741 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
    49801 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
    49861 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
    49921 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
    49981 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
    50041 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
    50101 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
    50161 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
    50221 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
    50281 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
    50341 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
    50401 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
    50461 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
    50521 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
    50581 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
    50641 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
    50701 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
    50761 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
    50821 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
    50881 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
    50941 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
    51001 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
    51061 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
    51121 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
    51181 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
    51241 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
    51301 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
    51361 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
    51421 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
    51481 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
    51541 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
    51601 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
    51661 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
    51721 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
    51781 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
    51841 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
    51901 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
    51961 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
    52021 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
    52081 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
    52141 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
    52201 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
    52261 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
    52321 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
    52381 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
    52441 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
    52501 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
    52561 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
    52621 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
    52681 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
    52741 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
    52801 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
    52861 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
    52921 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
    52981 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
    53041 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
    53101 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
    53161 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
    53221 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
    53281 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
    53341 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
    53401 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
    53461 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
    53521 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
    53581 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
    53641 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
    53701 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
    53761 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
    53821 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
    53881 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
    53941 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
    54001 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
    54061 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
    54121 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
    54181 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
    54241 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
    54301 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
    54361 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
    54421 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
    54481 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
    54541 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
    54601 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
    54661 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
    54721 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
    54781 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
    54841 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
    54901 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
    54961 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
    55021 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
    55081 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
    55141 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
    55201 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
    55261 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
    55321 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
    55381 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
    55441 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
    55501 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
    55561 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
    55621 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
    55681 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
    55741 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
    55801 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
    55861 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
    55921 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
    55981 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
    56041 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
    56101 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
    56161 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
    56221 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
    56281 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
    56341 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
    56401 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
    56461 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
    56521 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
    56581 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
    56641 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
    56701 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
    56761 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
    56821 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
    56881 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
    56941 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
    57001 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
    57061 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
    57121 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
    57181 ccctccatat tatgttggct ctttattccc actctgtttt caacgtaaag tgtataaaac
    57241 agaatgcgat actatcaagg gagttaaaac aatatacaga acagtaaaac actataaata
    57301 aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat catactctgt ttatatatga
    57361 aaactagatt ctgaatccaa tatttggtta acgagaggcg ttcgaatttg aacgtctttt
    57421 atatattact ttcaaataaa tatgaggtag aatactatct attttctttt taacccaatt
    57481 gttataatgg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc ccttctgtag
    57541 aatagagtta ccacaacaca ttcacagaag agaggacttc cctatgaacg attttactac
    57601 agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc gtgtccattt
    57661 ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag attacgaaaa
    57721 gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg accgtacggt
    57781 caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa
    57841 gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga ccgtcattca
    57901 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
    57961 gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag aagaggtaac
    58021 ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg acgcaacgta
    58081 tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag ttggcattcg
    58141 cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat ccatcacgat
    58201 ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat
    58261 cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca aagctcgttt
    58321 tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg
    58381 taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt
    58441 ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc
    58501 gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca tacgcaagag
    58561 tatttattct acgaacttga ttgaatcctt taataaacaa atcaagaaat acagccaccg
    58621 caaggaacag ttccaaaatg aagagtcgat ggaacgtttc ttagtctcgt cttttgatac
    58681 ttacaaccaa aaattcctag gtcgcagtca taaaggtttc caacaggccg aaggcgagct
    58741 cgaacaaatg ctaagccaac tgattgagaa ttagaaacaa tctgcagtag ggaagaatca
    58801 tttacacaaa attattgacg ctcccttata atgagaactt ttactaacat aatttatgat
    58861 aaacttacta tataatcata ttaattaaat agttaggaga tctgcttatg tttatatcta
    58921 ctcttcaaat tcgaaatttt agaaattatg aatccgaaat ctttcgattc gataatgaga
    58981 caaatactat tataggtgag aatgattcag ggaaaaccaa tgctcttact gcattgagaa
    59041 tcttattaga tgatagttat tactatagta gtaaaacact aaaagaatca gatttttttc
    59101 atggaattca aaatgggtgg caagggcatt ggattattat ctctgcgaca tttgaaggaa
    59161 tcagcgaaga agaatttgac aatgagattt gtgcttccat ttctttaaat tctgaaagtc
    59221 aaacaatctt agaagaattg atttcaaatg cagataaagg tgtaggtagt atttctctat
    59281 ttattagacc taacaaagct attagaaagc aattatttga tatttccact gaattagact
    59341 ctcatcaatt taacgaattt agaaattcta ttcgtctatc cgactatgaa ttttactaca
    59401 catctaaatc aaatctaaat ttctgtatca atgaaaatta tgataaattg gtaggaagac
    59461 tcaatgacaa tatagctagt aatcctgaag aagacgatga agctctgttg ggctcaagaa
    59521 ttgacatgtc agatattttc aagcatattt cggttgttta tgttgacgct cttcgagatg
    59581 ttcttagaga aatgaaaaac aatcgaaacc cagtcaaaag aatcatggaa acaattgaat
    59641 caaaaatatc atcagataat gttgagtcat taaaaacaat tatacagcaa ttaaatgaga
    59701 ctattaccag tgttcctgaa attaggaaaa ttggcgaaaa tattaatcgg caactaaata
    59761 gcataattgg ttcagtgtat tctcctaatc ttcttctttc ctcaactatg tcagatgata
    59821 tgggttcttt agcaaaattt ctttcaatga agccagaaca aaatatcgac ttagacttac
    59881 tagggttagg acatctaaat atgatttatt tagctttgaa aatagtcgaa tttgaagcgt
    59941 gtcgttcaag agaactactt aatattatgt tgattgaaga accagaagct catattcatc
    60001 atcatattca aaaaactctg ttcgaaggat taaatcttca aaaaaactat actcagattc
    60061 tcatgaccac acattctgta catttagctg aagtgtctga aatatctcgg atgaatgtat
    60121 taaaatcgtt taatggcaaa tctatcgtta tgaatccagc aaataatctg aactcatttg
    60181 ctaaagaaaa gttaaaaaag aacaatctaa atctaataaa cgcagtagag agatatttag
    60241 atgttaaaag aaatggattg cttttttcta aaggtgttat cttagttgaa ggagatgctg
    60301 aagaaatact tattcctcaa atagtaaaaa aattatttgg tataaactta gacgaacttg
    60361 gaataggttt gattaatgtt ggtagtactt cgtttgaata tatcgctagc ttgtttgacg
    60421 atgatagaat acagagaaag tgtgctattg taactgattt agatgaacag gcaatcccat
    60481 cagaacatcg attgtataaa agcactgcac aagagaaagg tcaagcaaga aaagaaaaat
    60541 tatcctccct ttttaatgaa aacaattggg ttaattgttt ttatgctaat acaacatttg
    60601 aaattgagtt tttagatgtg aataaagaaa atattctaac ttatgtcagt ccaattatag
    60661 atgacgcatt caaagataaa aaaacaataa ctgcttataa agaagaaata caaagtgaac
    60721 aatatttaga aagaaattta gcgatgttaa aactagccaa tcatgttggt aaggggtggt
    60781 ttgcaattga gttaggtgaa aaaattgact ttttaatcaa aattcctaag tatattctta
    60841 atgctattgc ttttgcatgt caggaaatag tagatctctc aatttataaa aaaattctac
    60901 tccatacaat cgacttgtac gaggaaacta cagaattgtc agctttatca gctagtttaa
    60961 aaacaagtga tacagctgat ttgaatatag caataatttc tttattagag tactttggta
    61021 aagatgagga tgttgtgtat ctaatcaatc agatagaaaa ttacacaaaa atactttatg
    61081 ctgaggtttc aaaatgacaa atatcttcga aaaaatcgta aatgatttaa atttagaaca
    61141 aaaagatgtt gtggaaagtg aagataacct ttatgtaaat gcatgtcctg ggagtgggaa
    61201 aacaagggta cttacaagga aaattgctta ccaagcaatt aaacacgatg gatcactaaa
    61261 aagaataatt gctatcacat acactaatag agctgctgaa gaaattaagg aacgcctaag
    61321 tttacttagt atagatgatg atattaatat ttgggtgggg acaatacatc agttttgtct
    61381 tgaatttatt atttatcctt ttaaaatgaa tctctcaaga gtatcaaaag gatttacaat
    61441 tattgatgat tatactcaaa gagcttacgt cagccaaatt aataatatgc tgaatttaaa
    61501 tctcaaaacg tatgaactga acaaaattga tttgtcactt tctcatgatt ttaaaattaa
    61561 tgaagttcag tatactcaag tttctgaaat gtatcacact atgttggaaa aaaataaaga
    61621 aattgatttt gatatgattt tactagtagc gtataaaatt ttaactgtaa acagtaacgt
    61681 tgcacgtaat attgctagta atataagggc gatttatgtg gatgaatttc aagatactag
    61741 agaattacaa tacaatgtaa tagctaaatt acttcaaaac aaccctcaaa ttcagtcaat
    61801 gtttgttgga gatattgacc aagcaattta tggatcatta gatggtatag ctaaatcagt
    61861 tgaagaacta gaaatgctaa ctagtcacac ttttcaatca aagacgctac atggctgtta
    61921 caggtctaat cagagattag tagacttcta ctctaatttt cagagttgtc cttacgagat
    61981 tgaaagcaga gggaataata aaaacgaccg tggttttata agttatgatt gcaaagttac
    62041 taaggaggat ttacccaaca taataaaaaa tataataaag gaaaagattg aaagcggcat
    62101 tcatgaaaaa gatatttgtg tacttgctcc tcaacactat cctctgtttt cactgggtga
    62161 gtatttaaag aaagaattac cgtattgcaa ttttgatgct ataaatatat caccaattaa
    62221 ggtaaataat catagtttat ttttcaagtt agcaattttg tactttactg aatcaggtga
    62281 aaaggttcga agaagaaagc taattgcaaa cgatatttta
//
LOCUS       DACDRK010000009        52621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDRK010000009 DACDRK010000000
VERSION     DACDRK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.009"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..429
                     /locus_tag="IWJ77_000662"
     CDS             <1..429
                     /locus_tag="IWJ77_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9452309.1"
                     /translation="KFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALMMGAVM
                     AVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIPHHSIA
                     ILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(933..2351)
                     /gene="nhaC"
                     /locus_tag="IWJ77_000663"
     CDS             complement(933..2351)
                     /gene="nhaC"
                     /locus_tag="IWJ77_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9452310.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(2754..3977)
                     /locus_tag="IWJ77_000664"
     CDS             complement(2754..3977)
                     /locus_tag="IWJ77_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9452311.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(4008..4847)
                     /locus_tag="IWJ77_000665"
     CDS             complement(4008..4847)
                     /locus_tag="IWJ77_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9452312.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4864..5544)
                     /locus_tag="IWJ77_000666"
     CDS             complement(4864..5544)
                     /locus_tag="IWJ77_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452313.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTIYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(5546..6619)
                     /locus_tag="IWJ77_000667"
     CDS             complement(5546..6619)
                     /locus_tag="IWJ77_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9452314.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(7266..7937)
                     /locus_tag="IWJ77_000668"
     CDS             complement(7266..7937)
                     /locus_tag="IWJ77_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9452315.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(8024..10702)
                     /gene="mgtA"
                     /locus_tag="IWJ77_000669"
     CDS             complement(8024..10702)
                     /gene="mgtA"
                     /locus_tag="IWJ77_000669"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9452316.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            11361..11798
                     /gene="ndk"
                     /locus_tag="IWJ77_000670"
     CDS             11361..11798
                     /gene="ndk"
                     /locus_tag="IWJ77_000670"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9452317.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(11820..12686)
                     /locus_tag="IWJ77_000671"
     CDS             complement(11820..12686)
                     /locus_tag="IWJ77_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9452318.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(12846..14939)
                     /locus_tag="IWJ77_000672"
     CDS             complement(12846..14939)
                     /locus_tag="IWJ77_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9452319.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(15688..16311)
                     /locus_tag="IWJ77_000673"
     CDS             complement(15688..16311)
                     /locus_tag="IWJ77_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452320.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(16470..17867)
                     /locus_tag="IWJ77_000674"
     CDS             complement(16470..17867)
                     /locus_tag="IWJ77_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9452321.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(17899..18870)
                     /locus_tag="IWJ77_000675"
     CDS             complement(17899..18870)
                     /locus_tag="IWJ77_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9452322.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            19244..19669
                     /locus_tag="IWJ77_000676"
     CDS             19244..19669
                     /locus_tag="IWJ77_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452323.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(20157..20858)
                     /locus_tag="IWJ77_000677"
     CDS             complement(20157..20858)
                     /locus_tag="IWJ77_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9452324.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(20874..21314)
                     /locus_tag="IWJ77_000678"
     CDS             complement(20874..21314)
                     /locus_tag="IWJ77_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9452325.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(21314..21889)
                     /locus_tag="IWJ77_000679"
     CDS             complement(21314..21889)
                     /locus_tag="IWJ77_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9452326.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(22737..23216)
                     /locus_tag="IWJ77_000680"
                     /pseudo
     CDS             complement(22737..23216)
                     /locus_tag="IWJ77_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(23375..24076)
                     /locus_tag="IWJ77_000681"
     CDS             complement(23375..24076)
                     /locus_tag="IWJ77_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9452327.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(24184..25593)
                     /locus_tag="IWJ77_000682"
     CDS             complement(24184..25593)
                     /locus_tag="IWJ77_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9452328.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(25641..27506)
                     /locus_tag="IWJ77_000683"
     CDS             complement(25641..27506)
                     /locus_tag="IWJ77_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9452329.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(27627..28478)
                     /locus_tag="IWJ77_000684"
     CDS             complement(27627..28478)
                     /locus_tag="IWJ77_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9452330.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(28788..29501)
                     /locus_tag="IWJ77_000685"
     CDS             complement(28788..29501)
                     /locus_tag="IWJ77_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9452331.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(29569..29895)
                     /locus_tag="IWJ77_000686"
     CDS             complement(29569..29895)
                     /locus_tag="IWJ77_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9452332.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(30018..30245)
                     /locus_tag="IWJ77_000687"
     CDS             complement(30018..30245)
                     /locus_tag="IWJ77_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9452333.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(30567..31820)
                     /locus_tag="IWJ77_000688"
     CDS             complement(30567..31820)
                     /locus_tag="IWJ77_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9452334.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            31881..32621
                     /locus_tag="IWJ77_000689"
     CDS             31881..32621
                     /locus_tag="IWJ77_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9452335.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(32707..33465)
                     /locus_tag="IWJ77_000690"
     CDS             complement(32707..33465)
                     /locus_tag="IWJ77_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9452336.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(33462..34490)
                     /locus_tag="IWJ77_000691"
                     /pseudo
     CDS             complement(33462..34490)
                     /locus_tag="IWJ77_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(34510..37677)
                     /locus_tag="IWJ77_000692"
     CDS             complement(34510..37677)
                     /locus_tag="IWJ77_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452337.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(37901..38785)
                     /locus_tag="IWJ77_000693"
     CDS             complement(37901..38785)
                     /locus_tag="IWJ77_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9452338.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(38880..39317)
                     /locus_tag="IWJ77_000694"
     CDS             complement(38880..39317)
                     /locus_tag="IWJ77_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452339.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(39534..40445)
                     /locus_tag="IWJ77_000695"
     CDS             complement(39534..40445)
                     /locus_tag="IWJ77_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9452340.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(40902..41330)
                     /locus_tag="IWJ77_000696"
     CDS             complement(40902..41330)
                     /locus_tag="IWJ77_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452341.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(41358..41996)
                     /locus_tag="IWJ77_000697"
     CDS             complement(41358..41996)
                     /locus_tag="IWJ77_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452342.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(42000..44036)
                     /locus_tag="IWJ77_000698"
     CDS             complement(42000..44036)
                     /locus_tag="IWJ77_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9452343.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            44162..44827
                     /locus_tag="IWJ77_000699"
     CDS             44162..44827
                     /locus_tag="IWJ77_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9452344.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(46762..49911)
                     /locus_tag="IWJ77_000700"
     CDS             complement(46762..49911)
                     /locus_tag="IWJ77_000700"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9452345.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(49924..51141)
                     /locus_tag="IWJ77_000701"
     CDS             complement(49924..51141)
                     /locus_tag="IWJ77_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9452346.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(51131..>52621)
                     /locus_tag="IWJ77_000702"
     CDS             complement(51131..>52621)
                     /locus_tag="IWJ77_000702"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9452347.1"
                     /translation="KYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEESKED
                     LIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVD
                     LQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGE
                     FYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQEL
                     NTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSAD
                     TTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIR
                     QKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNK
                     LSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHV
                     GNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT        15293 a        10916 c         8726 g        17686 t
ORIGIN      
        1 aagtttttgt tgatgatcgg tgtttcaacg gtagttatgt ttggtatgat gtacttgaat
       61 gtctatgcgc tcgatcatat cttcttcagt cgtacacgag tattcatggc gttgatgatg
      121 ggtgcagtca tggcagtcat catgctgttg ttcatgtgga aaatgtatga aaacaaaaag
      181 ataaatgtta tgatcttagc cgctagtctt cttgtttttg ccggttcctt gtttatggta
      241 cgaagccaaa ctactgtcaa cgacacagct tggatgaagg ccatgattcc tcatcattct
      301 atcgctatcc tggcaagtaa acgagctgat ttaaaagatc ctgaagtaaa aaaattagcg
      361 gaagaaatca ttcaggcaca agaagaagag atcgacaaaa tgaaaaaaat gatcaaagaa
      421 atggaatgac cattatccgt ccaaataaaa aaagaggatg ttccagaagt tatcagcttc
      481 aagaaataag aagaagctac ttctggaacg ccgtgaataa ggggttgtga aaaagctgcc
      541 ctgcatcaag taataagaac agccaaacaa aaatagcttc tcctattttt cgtttggtta
      601 ggcttattac cgcagatgca agcttttgaa caccgtttat taagaggttg taaaatcagc
      661 gttctgacct gagtattaga gagacaaatg gaaaaatagt tcctcatatt ttgccatttt
      721 ttgagctaat accgagggtc agctgattga acaccgttta tcgagaggtt gtgaaaaagt
      781 cgcttagttt cgcgtattaa gaaaaatttt gtgaaatggc tttccacatt tttgtataaa
      841 tttggcttaa taccgagaaa ctggcttttg aataccgttt attcgtaaaa gggattgtga
      901 caaaagtctt gtcacaatcc ctttttatgt ttttattgtt ctaattgcgc tgtcttgctt
      961 actgcttctt gcggtttcac aaattttttt gagattcccc aacctgtgaa ggcactgacc
     1021 atcgaaaaca caggagaaag caaactgaaa aatacgaatg gcaagtaagt gagtgtagga
     1081 ataccaaatg tacttgctac gaaacttccg gcaattcccc aaggcactaa gtagttgatt
     1141 actgttccac catcttctag cgttctacct aatacagttg gatcaaatcc tgctttttca
     1201 taagtttctt taaatgcatt accaggcaga ataacagata ggaattgttc accaataaaa
     1261 atatttactc cgatacttgt catcaatgtt gtaaaaatca aatttggtgt gttcgtcatc
     1321 tttcttgata ctcgttccat cacggttgag atcaagccaa attcgatcaa caatccacct
     1381 aatgacaaag ttagaataat caatgacaca gtaggcatca tactttcgat tcctccgcgg
     1441 ctaagcagtg aatcgatttg ttggtttcca gtatttgaaa caaaccctga ttcgatgacc
     1501 ccagccaact tcgttaagga agtttgtggg ttttgaataa aaatcattcc tactgctaca
     1561 gcgatattca acaaaatcgt taaaattgcc ggcacctttt tccatgcaca cagtaacatt
     1621 aaaaataaag gaatcagtga ccagattgaa atagtaaagt ttgcttctag aacatcagtc
     1681 acttcacgaa tagatgaaag acttgcggct ttacttgtgt gtcctaaaat catgaataaa
     1741 aagaaagaaa caacaaatgc cgggacagtt gaccacatca tatgcttgat gtgtttgaac
     1801 aaatcggcat caacaatagc tgctgctaga tttgttgatt cggataatgg agacatctta
     1861 tctccaaaaa cggcacctga aacaatagct ccaacaacca gcgctggatt taagcctagt
     1921 gttgtcccaa taccgaaaaa tgcaatccca atcgttgaca taacagtaaa ggcactccct
     1981 acagcgctac ctacgattgc acacacaaca aatacagaag ggacaaacca ctggacactg
     2041 atcaatttga acccaatgac cattagagtt ggaatgatcc ctgcttgtat ccatacagca
     2101 atcaatgaac cgactaaaat aaaaataaag attgggatga ttcctggttt gatgccattg
     2161 atgattcctt cgtgcagcca atctgtcggg tatcttttta tcactgcata caacatgacc
     2221 attccgatcg ctgctaagat cgtgatatta ggtgagacct ttaatccgac aacgccaatc
     2281 gcaatgccta aaacaataac cattaataca agtgttgctt ctaaaaaact tggtgttcct
     2341 ttttttccca tgataatctc tcctttttta gttgttctta tgttctaaaa aagttaagat
     2401 atcggattac tccgccgcaa ttcatccgcg attcctcctt tataaaaata aaaagacctg
     2461 ttttaaacta aggacgaatg actccgcggt accaccttaa ttaaaaacag atctgttttt
     2521 cactttcaaa cttttattca aatgtatgga ctttccatgt aattcgtcaa ttaggcttga
     2581 tcggttcaca gcgaccaccg actttctgaa caaaacacct aaccgctact gcgtgaaaag
     2641 ttgacttgta cgaagtttac cttccataag atcatttgtc aatgaaaaag aaaatatttt
     2701 ttcatttttc tgaaaattat ttttataact ttcacacaca agtttatttt ttactagaaa
     2761 gtagcgccct cttgtttttt tcttgctact atttcttgat gttcttgctt gattctttct
     2821 aatagctttg gctctttcaa cagatcaaga gctgtattgg cgagtagttc tgctgcaatc
     2881 gcaatagaag caaggccttt gtcactttta gcagcagctt tgaattcttc tgtgtgtccg
     2941 gcaatatatg tctcagagat tgctaccgta ggctggattg ttggtatgac ttggctgaca
     3001 tttcctacat cagaagaacc gagacttgtt ctttgttccg tttctatttc ttcatctgga
     3061 acacctgctt cttttgcatg ctggaagaat aagtcatcaa aagaaggagt aacgatgaca
     3121 tcatcaactg cattttggaa caatccaaac tcaaaagtcg tgcccgtact tatcgcagca
     3181 cctcgtacga tattttccac tttttcatat actttatcta atgtttgccg atttcctgca
     3241 cgtaaataga aacgtccagc agcgtaatct ggaatgacgt tggcagctac tcctccgtct
     3301 gtaatgatcc catggatatt gacatctttt ggcaattgca agcgtagagc gttgattcca
     3361 ttaaacactt ggatcaatgc ttccaaagca tttactcctt tttcaggtgc tgctgctgca
     3421 tgggaagcga caccgtaaaa tttgatgtcg actggatcat tcgctaaaga ttctgtcgtt
     3481 ttcccataac gaaaagctgg atggacgcat aacgctacat cgacatcctc aaaaaaacct
     3541 tctcgaacaa agctgccttt agctgatccg ttctcgcccc cttcttcccc tggggtgcca
     3601 tatacacgga tttctccacc agtttcatca atgaattgct tcactatgct ggcggctaat
     3661 acggagtaag taccaaacaa attatgcccg catgcatgac caatacctgg taaagcatcg
     3721 aattctgcca aaaaggcaat tgtgggaccg ggtttattgg aaatataacg tgcatcaaag
     3781 gctgttcggt gtccagctac ttctttttct acccaaaacc tttctttttc taattgttta
     3841 atcaacgctg ctgaggaata aaactcatag ttacttactt caggatgttc gtggatatct
     3901 agagcaagtg cttgatattc tggtaaacga tattggatta gatccttcaa ttggtttgtt
     3961 gagattgttg ttgtcataaa aaattcctcc tatgctagtc atttttctta ttttctcccg
     4021 aatgtttccc atgcaggcac gcttgatcct tttgatgttt tctcgattac tttgactgtg
     4081 tcttctgttt gataagcgtc tactattttt tgatacgttt tattgttttc atcttctttt
     4141 cttgcaacga tgatattcac ataaggacgt gcatggtcat ccactggttc taagaagatg
     4201 gcatcagaag caggattaaa tccagcatct acagctacac cgctattgat cactgaaatg
     4261 tctacatctg acaaagcacg tgctgtttgg gaagcatcta attcagaaat gtctagattt
     4321 tttctattct cggtaatatc agaaacagtg ggcgcttgtc cttttgcctt atccactttg
     4381 atcaatccag cagtttgcaa taacagaaga gctctgccgc catttgtaac atcattaggg
     4441 attgctactt ttgctccgtc tttgatatca gctacatcct tcaaagtgtt agaataaatt
     4501 cccagcggtg cattgacagt atttccgatc gatactaaat cagttccgtg ttctttattg
     4561 taattgtcta aaaagaattg atgttggaaa gaattcaaat caatcgatcc atctgctaaa
     4621 gccacatttg gttgattgta atctgtaaat ttcacgtatt ccaaattaat gttctccttt
     4681 ttcaaacggt ctttcacgtt gtcccataca tctgtatctt ctccaataat ccctagtttt
     4741 acgatagttt cttttttgtc agttgtatct gcttttcctt ggctacaccc tgccaaccaa
     4801 ataattgcta caaatgtaat gactacaaaa cgaataattt ttttcatttt ttctcctcca
     4861 ctttcaatgt gtaatttttt taatcagttg attactgata aattgactta aaaaaactaa
     4921 gattagaatc aataatgtag ccacaatcgt tacatctgtt tggaatcgat tataccctct
     4981 cgatatagca agattcccta atccgcctgc acctactgct ccagccatgg tagtcagtcc
     5041 gatcacgttg atgatagtca acgccgagac ccgaatgata ccaggcaaac cttctaacaa
     5101 atacacacgt aaaatgatac cgattggact ggttcccatc gcttctgccg cttcgatcac
     5161 cccaggatca acttctagca gtgcgttctc gatttgacga gcaaaaaacg gaacaactcc
     5221 aaaaatcaat ggcactaaag ctgctttcgc accaatcgtc gttccaacta agaaacgagt
     5281 agtcaatgct aaaagagaaa gcaagataat gaaagggatg gaacgaatga tattgatcag
     5341 cttatcaagt atttcaaata gcagacggtt agctagtaat ccattagggc gtgtcactac
     5401 taacgtcaca cctaagatga ttccaatcat cccgccaatg aatgctgtcc aaaaagacat
     5461 atatattgtt tgcttcgtcg cttcgacaaa ttcatccgga atcaaataaa cattgggcaa
     5521 atatgtttta aagaaatcag tcattttatc ttcctccttc gatcactttg atacgactat
     5581 ctaaatcgtt tttttgtaaa atatttattt ttacaccaga tttgatcaaa taatccaatg
     5641 cttttttccg ctgtttggtt tctccagcta aagtaacgat caaactccct aacggcgttt
     5701 cttgtaataa ctctacattg ccatataaaa tattcgctgt tacttgatat ttgctgtaca
     5761 gatgagcgat tagaggttcg ttcgtctgat ttccaatata acttaattcc accagccatt
     5821 tattggcgat ctgatcggca aaagcagaat gggcaatgat cgtttctaat gcttggtcaa
     5881 gatgagtagc tgttcgaata aaatctttgg tcaattcttc ttcaggattg ctgaagatct
     5941 ggatgctgct acctttttca acgattcttc catcttccat aaccgctacc ttgttgcaga
     6001 tttctttgac aacttgcatc tcatgagtga tcaggacgat tgttaaacct aactggcgat
     6061 tcaatttttt taacaatgca aggatctgaa gggtcgtttt cggatcaagt gcactggttg
     6121 cttcatcaca taataatacc ttaggttcat tcgccaaagc tcgtgcgatt gctactcgtt
     6181 gtttttgtcc ccccgacagt tgtttaggat aagcagatgc tttttcttcc aaaccgacaa
     6241 gttccagtag ttcattcact ttttgacgac gttcctgttt cgattttcca gaatacttta
     6301 aagaaaaatc aacgttgtca aaaatagttc ggctttccat tagattgaaa tgctgaaaga
     6361 tcataccgat ggcctttctt tcttttcgca attctttggg agagagctga ctcagattcg
     6421 tctgattgac aatgacttct cctgctgttg gtttctgcag caaattcatc acacgaacta
     6481 acgtactttt ccctgcacct gagtaaccga cgattccata aacatcgcct ttttcaatcg
     6541 ttaaatctac agaatctact gcctgtatct ctttttcttt ttgtttgaaa catacagaaa
     6601 catctcgcaa ttcaatcatt aaaattcctc cttatctaaa aaaagcgtcc tcaagaaaat
     6661 gaaaatgtca ttttcttgag gacgcttttt tgcgtgttac cacctcatgt tcaagctgtg
     6721 ttcacacaca actcctcttc aagtacgatc atactctagc aagatatcgg tcgcagccgt
     6781 catcgcctta catctatgct cgggatgaat tgctcgatgg ccattttcca atagtcgttc
     6841 cttgcttctt ctcagctacc gaagctctct gtcaaaggtt cttctatcgt acttaccatt
     6901 tcaatgcaaa taatattcaa aaaaaaagtc cttttactaa cataaactat gtcagtaaaa
     6961 ggacgacgta tcgcggtacc accttttttc ggaagtttcc ttccctcaag cccaacatct
     7021 caaaaagaga gattggcgtt cgttttaacg gtcgaacagt tccgaatttg cctacttatc
     7081 gacaaattat tctccaaggc cattttcagc ttttcttact tgactcttct caccagccag
     7141 agcctctctg caaagtttcc aaacttactt tccttttcaa cgaaatcgat attttgttgt
     7201 tgggattaat ctacatgagg aaaactgaaa agtcaagtac gaaattcaaa ttattttttc
     7261 tatatttatt tttctgtttt tttatttaac atttcaataa cagttcgaag atcttttcct
     7321 tccgcaagaa gtttcaattt ttccttttct tcgacggtat aatttcggac aaaactggaa
     7381 tcaggtatca aaacaacgcc taatcctgct cgtgaagcag ctgttgctcc tactaaagaa
     7441 tcttccactg caaaggctga actagcttct aatcctgttc gttccaaagc tgtcagataa
     7501 ggaatcggtg aaggcttgtt ctccgacact tcgtctccga atgtaaacgt gtccaagtaa
     7561 tcagctaatt taaagtgatc caagatatct gttgctcttt tcgtaacagt ggtcgtagca
     7621 atgcctagtt tcatatgacg ttcttttaag tctgccaata tttcttttgc atatggtttt
     7681 aagtggatgg ctccgcttgt cagctgttga taaaaatatt cttcacgctt cgctcggact
     7741 tcctgtactt tttcagggct acctgctgct ttcactaaaa tatcaaaact ttgtttccag
     7801 ttttttccgc cccaagcgtc aacgatttct tttgggatat ggatatcaaa ttgctgaaat
     7861 ccccaaagcc agccatcacg atacgttttt tctgtgtcaa ctaacaaacc atctaaatca
     7921 aatattgcac ctttcatcta tacattaatc ctttccaaaa caatacacat agtttagcac
     7981 gactggtcca aaaagaacag aagcaaagaa ccagccttct cgtttataag agttcttgga
     8041 atcgacggat atagattttc ttcacagctg tcacaagcgc cagataagcg atgactgtag
     8101 caaataacca taaccagtaa ttgccaggaa gtggcatcaa gcctaattca gcaccaaaac
     8161 ttgtaaaagg aaggacagaa ccgacaataa ttcctaatgt agtgataaca aacatggcaa
     8221 atgttgcgtt gctttggata aatggtactt tcggtgtacg cagtgcatgg agcactaagg
     8281 tttgtgtcca tagtgattcg acaaaccatc ctgcatggaa taatgcgatg aaaatcactc
     8341 gctgtgaact atccaatgta tggaaacttc cgcccactac agcaggacaa ataacaaaat
     8401 acatcaacaa gtaagtagta atatcaaata tcgaacttgt tggaccaaag taaaccatga
     8461 attccccaat actagatgca ttccattttt ttggctcgtc caaatattct ttatccatcc
     8521 gatcccatgg caaggaaatg caggaaacat catagatcag attcaaaaac aaaagttgta
     8581 aaggcagcat tggtaaaaat ggcaggaacg tacttgcaat cagcacggaa aacatattgc
     8641 caaaattaga acttgctgta gccttgatat atttcatgat attcccaaaa gtttctcgtc
     8701 cagaaagaag gcctctttcc aaaatcgtca aatctttttc taaaaggatc acgtctgcag
     8761 attctttcgc aatatccaca gctgtatcta cagaaatacc gatatcagcc actttcattg
     8821 caggagcgtc attgatacca tctcctaaaa atccgacagt atgaccattt ttacgtaaag
     8881 ctgtcgtcaa acgtgctttt tgctgaggat tcaattttac gaatacttca tggtcttcag
     8941 ctacttgtgc taattcatga tcactcattt ccgagatttt ctcaccggta accaattcat
     9001 tgatctctag acctacttga cggcacactg aacgtgtaac taattcgttg tccccagtca
     9061 gaacttttac agctacacca tgttctttca gagctttcaa cgcttgtttc gttgtttctt
     9121 ttggtggatc aaggaaagca agataaccaa tcaaaaccat gtctgactca tctttgactg
     9181 aaaattctcc aacgacgctt ggattggtct tttgtgcgac accaatcact cgtaagccat
     9241 cttcattaag tgactgtact tttgcaagta cggcttcttt tcgttctttt gttaatggtg
     9301 taacagtacc gccaagatca ataaatttag aaatgttcag catttcttca atcgcgcctt
     9361 ttgtgatcat ttgtgtttta ccagctggat cagcaaccac aacactcata cgacgacgtt
     9421 caaaatcaaa aggaatttca tccacttttt gataattgat cttatctgta tctaaagttt
     9481 ctgttgcttc atcaatgata gccacatcta acaaattttt cagccctgtt tgatagtagc
     9541 tatttagata cgcatggcgc aaaacacgat catcttcatt tccttcacaa tccatatgat
     9601 attcaagaat gattttatct tgtgtcaatg tccccgtttt atccgtacat aacacatcga
     9661 ttgcaccaaa gttctggatg gcattcaaat ttttgatcac tgttcctttt ttggccatca
     9721 cacttgctcc tttgaccaaa ttcgtcgtaa cgatcattgg aagcatttca ggtgttagac
     9781 caactgctac agatagtcca aaaagaaaag cttctagcca atcccctttt gtaagcccat
     9841 tgatgacgat aactgctgga gccatcaatg acataaattt gatcagtagc atggatgttt
     9901 tatggatgcc taattcaaag cttgattgaa ttggtttttc agatagcgtt tctgctacat
     9961 gaccaaatag tgtttcattt ccaacagaaa caatgattcc ttccgcactt ccactgatga
    10021 cattacttcc cataaagaca agattttcgt aacttgtttc gctttgctga tcgtcttcac
    10081 atactttcgc atgcttttct actggatagc tttcccctgt caatgctgct tgggaaatga
    10141 ataaatcctt tgcggacaat aaacgcatat ccgctggtat catatcccct gctgaaagcc
    10201 gaacaagatc tccacagacg atttcttcca taggaatctc tgtgtacttt ccattacgtt
    10261 ttacagctgc cgttactttc acaagtgatt tcagtttttc agccgcttga ttggaacgta
    10321 cggactgaat caacgtcatt gtcccgctga cgataaccat agtgaagata atggctacac
    10381 cgaaaagatc tttatctcca ggagctgcta atacatattc cgtgatgaat gaaataattc
    10441 ctaatgcgat caacactaat gtaaatggcg tgatgtatgc tttcaatatt tctacaatga
    10501 atggtgtttt tttgccatat gaaatcgcat tttcaccgta attttctctt gaacgttcga
    10561 tttgttcttc tgtcaatcca tttgctgatg catggaatag ttcaaataat tcttgtttcg
    10621 tttttccagc aaaagctgca tagatttttt gtgtatcttt ctttttttcc atctcaatct
    10681 ctcctttgtg tccaaaagac agctgttctc gctgcttttg gtctttagct attcatcgga
    10741 tcgatagaga aaaaactgaa aggggctgag aacagataga taatagccct ggcctttcct
    10801 ctagatcctt gtttttcgaa tgactgtcat ccatttgttc tcatctcctt tactaagtaa
    10861 ttttaattca tgacttccaa tcatttttcg aatagttatt tttccaatta gaccacctcc
    10921 tttttatttt tcaccgatgg ggatttgcgg caacaaaaaa acctctcgcc aaatactcag
    10981 cgaaaggttt gaaaaagcat attaaaaaaa gcttctcaga tctcatcgtt gagttttgac
    11041 actgtacaac gtaaaaagca gatacctgct tttagctacc ttatgaaaag ccttatctcg
    11101 acaattcctg ttctacccat tggcgtctct cgacgttttt gggcagtagc ctatgtttgt
    11161 ataggagcct cacctaacaa atcttattcg gttttccttc gttaatataa caactaaaaa
    11221 agaggatgtc aaatcaattt tccatgtttt tcatactttt tttaaaatac acacgttata
    11281 ttcctgtctc acgcggtttt atttgtctca ttttccacct cccgctataa tagaaatata
    11341 gtaggaaacg aggtgctgtt atggaaagaa cacttgttat tatcaagcca gacggcgtga
    11401 gacgccattt agtcggaagc atcatccaac gttttgaagc aaagggactg gccattgctg
    11461 aaatgaagtt tgaaactatg actcctgaat tagcaaaaga acattatcag cacttgactg
    11521 agcgcagttt ttttgatgaa ctgatcgatt acatgacttc cggtcctgtc gtttatcttg
    11581 tgctggtcgg cgaggaagtg atcgatatcg tccgtaagat ggttggagca acaaaagctg
    11641 ccgatgctgt tccaggtacg atccgaggag actacgcgct gccggggact gaaaacatca
    11701 ttcacgcttc tgattcccgt gatgctgcgg taaaagagat cgcccgcttt ttcccgccat
    11761 tcgatataaa caaaccgtct gaaaaagcgg cgaagtgata caaattctaa actgctggtc
    11821 tagttgctgg gttcagcaat ggccagcagt tttttctcta aggacctccg ttgattcttt
    11881 cctaacatat attcgagcac tgtgaagaat tcttggtagt ctttcgtctt ttcttcccct
    11941 aaagcagcag aaatcaatct ttgataataa tctgccatca atgtttcata gatcactgta
    12001 cttctttctt tatgtgctaa gctccgtttc atatcttcat tcacactacg tgcctttttc
    12061 aaccgaccat tttctatata aaacgaagtc aggttcatta aaaaagtaaa gagatactgc
    12121 tcatgattgt tcaactgtgc taatcgtcgc attctttttt tgattttcaa ataattgttc
    12181 tcaatgaacc gatcactgaa tatccaagga aaatcagcta gaatagatag ttcaaattga
    12241 ccatagtctg gtatctgcat caagtattct tcaatgattc ggcattcata acgtaaaaat
    12301 tcaaattctg tctcagatac ttctgttttt tcgaccatgt gaagattcaa aaccaaataa
    12361 tacagatctt ctgttttctt aaaatgccta cggttattgc tgatctcttt ataaggatca
    12421 aagctgtctt cttgtacccg atctagcatt cgtctttttt tctttaactt tattggttca
    12481 tccagctggt ttcgcatcca ttcatattct gcaagtgtca tgttcattcg ctctaaaaat
    12541 gatctcaatg ttacaaatga catttttttc actgaatttg tctcaatccc gactagagaa
    12601 gtcttaaaac agattccttc cgctaattgg gactgtgtca acttcttgtt gactcgaagt
    12661 ttcttgatca attcaccatc atacataata gttctccttt ttaatttaaa tttttccaca
    12721 ttcaataaat agatacgtta aaaaggaaaa atacacgaaa aaaataaaaa aatgtttttt
    12781 tcataaaaaa taccaacctt caagcattag aatgttgtct aactactgaa ggttggtttg
    12841 attgtttatt gattattttt ttgctgttca acgtctgttc ttgttactgg atataagttg
    12901 gttgttgttt tattaccatt ttttgaaaag acacttgtcg aatgttcgcc tttttgcttt
    12961 tcgattgctt cattacgttc cgcttcattt ttatagtcat attttttcgg atcaatcgtc
    13021 tcaaatccgt ttggcacata aaaccgcaag agattttcct gattcagctt atctgaaaga
    13081 cttaacgcag tcttgacttt ttcctgatct ttggagattt cctcatcgat tcctgctgct
    13141 ttcgtatcga tgatttctcc tgtctgatta ttgtaagctt ttcctcctag gacggtgtat
    13201 ttgggtgtca caaagttccc atttcgaaaa gcaactactt gatcatgctg agaagataat
    13261 aagtctgtac caaaatgaag ataatcttta tcatcgattc ccaatagatg tagtaatgtc
    13321 ggcaaaacat cgatttctcc accgtattca tgattgactg tccctttagt ataaccaggc
    13381 atatggatca tcaatggcac tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct
    13441 ggatctttac caagagcgct tgctaaatct ttattatctg tattcgagat cccaaaatga
    13501 tccccgtaaa tcacaaagat tgaattttgg taaataccag attttttcaa atacgtaaaa
    13561 aattgttcca atgattgatc cagataatgt gccgttcgaa cataattatc tactgtgcta
    13621 ttgccagtat tcaaagatgg aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg
    13681 ttggttacgg aaagaaattt cgtgtaaaac ggctgttgta gatgctcaag atattgggca
    13741 gattctctga ataaatcttt atctaatatc ccataaccta acgtttcgtc ggattcatca
    13801 aaataagatc gatcaaagaa attgtcataa cctaagtttt tatatacatg atctctattc
    13861 caaaaactag ctacattccc atggaaaaca gcgcttgtat agccttgctt ttgtccaagg
    13921 atcgctggtg cggcttgaaa gacattgtct gacccaagtt ctgtgaataa tgagccttga
    13981 ggtaaaccaa atgtgccagt ttccaacata ttttcagcat cacttgtctt tccctgtcct
    14041 acttcatgaa agaaattgtc aaagctgatc gttgcttgat tctggaaaat actatttaaa
    14101 aacggtgtga cttcttgtcc gtctaccttc atattaatca aaaactgctg gaaactttct
    14161 aaatggagaa cgatcacatt tttcccttta gcgatcccaa atgtttcggg atttggctct
    14221 gcataatgtt tctttgtata atctaaaact tcatcaatcc cattactgct tgcatgcgcg
    14281 cgaacactgc tagtcattcc tgttttgatt ccgtcataaa tagtatacgc atcgattcct
    14341 agatatttga caatatatga acggtcaaat gttcgctgca gcaattgtgg acggttggct
    14401 tcgctcagcg ctagatttcc tgaaaaaata agccctgaaa gacaggtaac agcgatagcc
    14461 atcggtttgc cgacagggtt gcttttgata ggtattttct tgaccttcaa ccaaaccagt
    14521 agcccaatga acacagcgat atccagccag tagatgacat catggggctt catcaaagaa
    14581 aaggagcttc ctgacaatcc ttgcgacact ttcgaaaagc ctaatacaga tttgactgta
    14641 atgaaatctg tgaattcccg ataaaaaata atattcaagt acaacaaaag cgtatttaaa
    14701 atatcaatga tcaaaagaat agctaacgct ggctttggct ttttaatata caaagaaagg
    14761 ccaaaaaaca ataaggtagt cgcaattggg ttgatccaca agataaagta ctggatcgta
    14821 ccttccacac ctaatgaaaa atctgcatag taggcaatga tcgtcttcag ccagaaaaag
    14881 aaaacagctg tcaaaacgat tgctaaacga ttttggcaca attttttaag ttgtgtcacg
    14941 tatatcactt ctctctattc acattttttc ttattttgct cctgaaaaat gaaaatttcc
    15001 taaaaaagtt gttatcttaa gattttttta ttctttctaa agattattcc gtccactttt
    15061 cacagcataa aaccgctgcg atgtaagcga ttaaactaat gaagagataa aagtcttgtg
    15121 gtgaccgctt cactccacgg tattcaaaag ccagtttctc ggtattcgta caaaaaaaga
    15181 ggctgtgaca aaattcttgt cgcagctcct tatttcttga tgcaggacag ctttttcaca
    15241 acttcttcct taaacggtgt tctcctgcca actcctcggc cacaagtcac aatatttata
    15301 aaacatgaga agctgttttt tgaaattgtt ccttgtggct cagagttaaa cggcaggctc
    15361 aagacctctt gatacacggt gttcaatcag ctgaccctcg gtattagatt atgtcaggac
    15421 gctaatttta caacctcttg gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc
    15481 caaccaaacg aaaaatataa ggagctattt tttgaaattg ctccttgtgg ctcagagtta
    15541 aacggcaggc tcaagacctc ttgatacacg gtgttcaatc agctgacctt cggtattagc
    15601 tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca
    15661 aaacgctgat ttcacaacct ctgtttttca ttgtccatag gattgatatt gtccttgtga
    15721 tgactcattc gtaataattg aggcttgatt ttcttcactt cccgttatcg tttgtccact
    15781 gccagaatct gaaggacttg ttcctgtcga ctcgtttttt tgagaagaga cagttgtttt
    15841 cttgacttgt tcatttgtct ttttccattc cgctaaacga tcggcgacca cttttttgtc
    15901 tactttttca gaataagcgg tgatcacttt tccatttttc aaacgggcta ctttgtcgcc
    15961 atcatgagtc aaaagcaaaa cggtcaaatg cttgtcttta aattgatatt gtctgagaga
    16021 aatcgcacta agtacgtcat cgatattaat aaattgattt tcttcttttc cttttggcaa
    16081 ataaatgtcc aattcgcctt tttcgtaatt ggcagttatt ttatactccg cccaccaata
    16141 agttgagaat tcttttttga aattatctac tgattctttg cttgctacga ttttttctgt
    16201 ttctttctga gcaagatctg ctgtcatttt tcctgaaaat gctgcggata aaataagcag
    16261 taagaaaaaa ccgaataacc aaaagagaag aaacggtctt tttttcctca tgctttcctc
    16321 cactcctatt taattttgac tattctatta tatacaaaaa atgaaataga aagacaaaaa
    16381 agttgaaaaa gatcgtaaat aattgaactt ctttttcaca aaatggtaag aatggagaat
    16441 ttcacgcatt tgctctcaac tattctttct taagcctcaa atccgttttt atctgatacg
    16501 tctttgaacc aatgacccga ttttttgatt gttctttttc catcttgtgc taagtctact
    16561 gaaataaagc catatcgatt cttatatgca ttggtccacg accagttatc catacatgtc
    16621 cacatatgat agcctaaaca attggcacct tcttgaatgg cttgatggac atatttcaga
    16681 tgagattgga caaattcgat ccgataatca tcgtggatca tgccatcttc tttgataaat
    16741 cgtgcttcgt tttctacacc cataccgttt tcagaaataa agcagtcgat atttccataa
    16801 tgatctcgta cattgatcaa aatatcatag attccttgtt catagatctc ccatccgcga
    16861 taaggattca ttttacgacc aggcatttca taattgtcga aataatcctc aggcattgga
    16921 ccgtttttag tatcgattgg tgtttctttt gcttttaccc ggcgtggctg gtaataattg
    16981 atacctaaaa gatctactgt attttctttg ataaccgtca aatcctctgc atggatcaca
    17041 ggcatgagat caagttcttt tactatctca atcaattcac ttggaaactc gccttttaca
    17101 gctggatcaa ggaatgaacg attgaagaac gcatctgcta tttgcgctgc tttaacatct
    17161 tcagggtttg attcgtctct tggatagctt ggtgtcagat tcaagatgat accaataaca
    17221 ccaccttgct ccatttcatg gtaagatttg atggctaacg ctgaagccaa cgcttcatga
    17281 tagccaactt ggatcgcttt tttcatatct gactcttctg gataatgcca tcgatataaa
    17341 taacctgctt cgactggaac gattggttca ttatgagtaa accatttttt tactttcgaa
    17401 ccaaacaatt caaaacaact tttggcatat ttttcatagg cttcaaccgt ttctcggttc
    17461 aaccatccgc ctttttcttg cagatacatc ggcatatcga aatgatacag attcacaaag
    17521 ggttcgatac cgttttcaat caactcatca aaataacgat gatagaaacg aacggcttct
    17581 tcgttgattg ttccagattc atcaggtatc aaacggctcc attggataga tgttcgaaat
    17641 gaattatgcc cagtctgttt catcaactgt acatcctctt gatacttcgt ataaacttgc
    17701 gaggtttttt caggtcctac ttgatcaaag aacttttgcg gttctttttt gtaccaataa
    17761 tcaaaaatgt tctctccttt tccatcgcct gaaatccttc cttctgtttg tgggccactt
    17821 gctgcagaac cccaccaaaa atttgctgga aattgatatt ttttcatcaa agcacctctt
    17881 tttctttttt agttcgtttt agttgattcc atgtatgata ttttccatca caatcacttt
    17941 ttcattttct tcctcttctt ttttgattcg ttttaacagc tgttctgctg ctcgttcccc
    18001 gattttatct gttggtggcg tgatcgtgat tttcggaagg atactcagat cgtttttatc
    18061 taaataatca tagtgactga acgcaatatc cttcccgatt ctgacacctt cttgattgaa
    18121 acattcgatg gcaccaatcg acatattgta attgctgaca aaaatagcag ttgccccttt
    18181 ttctaataat gcttttgccc cgtaatagcc gctttttcta gaatagtctc ctgtaaagac
    18241 ctctaattgg atcgatggat cattttggac cgcttctttg gctcctgcta aacgttctct
    18301 tgctacgtag tctgtttgag tggcagcaat aaatccgatt tttttatgtc cttgttcacc
    18361 atgatgagcg atcacttttg ctacactttc tcgatcattc actaatattg tatcaatacc
    18421 tgctggtcct tctggacaat tcaatgcgat aactggaata ctcatttttg tgagcatttc
    18481 aaatccagac cactcttctt cggataagaa gacaatcagc ccatcgatca catgcttcat
    18541 caggtattca attttttgtt caattttttc tggctgatcg gcaaacccgc ttaataataa
    18601 gctataccca ttttcttcag ctactctttc gatccctgag acaacttccg acccaaaacg
    18661 gctggaaatc ccgtttatca aaagtccaat agagaagctt cgattattct tcaacccttt
    18721 agcaatgatg ttttctttat agtccaacgc tgaaatcgct tctgctattt ttttcatgtt
    18781 cttttccttc agttgctgac cattcaagta acgggaaact gttcccactg aaacaccagc
    18841 catcttagcc acatcacgga tcgatgccat acattctcac tcctgccagt tacatttgat
    18901 ttctctttct tgttgttcga ttatgatgac cgcattcttt gcatagtgtt gcttgtcatc
    18961 aatatccata taactaaagt cttcagtaga gcaacttcct ctcactatta attatactta
    19021 aacaagtatt tgaaccggtt catttttaat atagcatttt tttaaaagat tgcaacactt
    19081 ttttaaaacg ctttctgaaa gtggcggatt ttttgccttt ttaaaatttt attctaaaat
    19141 ttactctatt tgtgggtgtt ttgtgcgttt ttctcttcca tccgtttgaa gttttacatt
    19201 ccaatggtat aatttcttta tcctatcaaa tgggggaagt attttgaaga aattaatcgt
    19261 actatttatt ttgtctgcgg gacttttgac attgactggc tgtgatttgt ttggcggctc
    19321 tgaccaatcc tcggacgaaa caaccgattc ttctgtctta aaagcggaaa aagcagatac
    19381 caccaacagt gtatatacgc taaaagtacg taaagatatg gatttatccg cgggacagtt
    19441 gacatttaca gataaaaaaa tggaatggac acgcacgtat cagacacaag gaactgattc
    19501 ttctgaatca tctgaagaaa aaacagtgct gacagatatc aagatcacta ccaaagatga
    19561 cgaatatatt atttcaggaa aagaaaataa aaaagaaaca acagtcatat ttacaaaaat
    19621 cggtaattac cgtttgaagg atgaggatgg caacatctac agtctatagt gccgtctgtt
    19681 ggatcttctt ttgtttctcc ccttcttttc tgctatagta aagaagcaag atgattgtaa
    19741 aggagacgaa atggatgatc gatagtcctg attattgtac atgtaataac aacaaaaata
    19801 tccatacaga atttaatgac caaggaagct tgagcatatg cgacaactgt cacaaactag
    19861 tcaaagattc atttaaaaca tttgaatctt atcctgttca aaattatgta aaaagtagaa
    19921 gaagataaac aaatccaagc aaaactcact atggctgatt tttatgaact tcaaactcag
    19981 cttcttcctt tgatcaagct tcatcagtaa aacaaacagt ggatcatttc agtttacaaa
    20041 tcaaagagta aagatttatt tatttcaaat atttgaatat aaaataacca aacgatagaa
    20101 aggcttattc tacatcacgt agtaattttt ctttcccgtc gtttggtttt ttatttttag
    20161 cctaattctt gaccttttgc atgctggtta tccgtaaaat attttccagc aggctttcct
    20221 tccaattcag ctaatttttc ttggatctga cggatcacct tttttgcatc ttctggattt
    20281 tctacaaaat cgaatttatc cccatccact tgtattttag gacatacatc aaaatcttca
    20341 taccattgat tatagcgggt gttcaacgtt tcataatagg tatataacgt ctcatcgctt
    20401 tctagctgtt catagtcacg cccacgtttt ttgatacgtt ctagcatcgt gtccaaagaa
    20461 actttgatat ggaccatcaa atcaggccgt ttttttggag caacatcatc taactcgttt
    20521 agcatcgtat ctaataagct atcgtactgc tgtacttcga tatccgttac ccgacctaaa
    20581 tcagcgttta gatggaacaa caggctatct tcatagatgg atcggtctag cacattgtca
    20641 tcgttcacta aagcattttt cattgctaaa aatcgcttgt tcaaaaagaa gatttgcaat
    20701 aaaaaagcgt actgctcagg atttgaataa aagagcggca atacttcgtt atcttcgaca
    20761 ttttcataaa atggtttaga atcaatctct tttgcaagca tatccgtcaa actgctctta
    20821 cctgcaccaa tcgttcctga aatcaaaatc actgacatac aacccttcct cttctataag
    20881 tactcgccta cgtaagtttc aacaggcaat gtttcaaaat catattcacg tacagcttgg
    20941 tctgatttta aatacgtatg gagactttgt gtaatcatca agttcgtagg taccgtctct
    21001 tcttgtacaa tgatcatcgg ttttttcaac atagttgcca cacctaattc gtaagccgtt
    21061 cctggatcga ttgtttgatg ctcaaagtca aggatagcaa caacaaattc tgcatttgtc
    21121 agttcttcca tatctttttc gtatacttcc tgcgcccatc cagcgctgaa tagttcataa
    21181 ttgctttctt ggtgatgacg tggagagtag aaacttgtga ctgttttgtt ttcctccaac
    21241 gctttttcga tacgacttac tcgatcgatt tgctcctctg aaaaaaatgg tcctgctaaa
    21301 tatatttgac tcattatttt gcctccttca ataataactg atttttaata gatactgctc
    21361 gtaattcatt gaccagttcc aatggattgt tggaaaagat tacttgatcc aaccattttt
    21421 tatccataaa atcctctttt gcaaaagttt ctaataattg ttgcaagccg ctgtaataac
    21481 cgccaatatt caatattccg attggcttgt cgggcgtcag accgattgct atccaagaaa
    21541 gaagttgtga gaattcctcc aatgtaccta gacctccagg gaaaacgagg acagcatctc
    21601 ccttttcaaa taataatacc tttctttcat cgatcgtttc tgtaggaata aatgtagtaa
    21661 catcttttcc aggcaattca gtttcaaaaa gcccattcgg atagatacct gtgacttttc
    21721 cgttattttc cagtactgtt ttactaatca ctcccataat cccggagtat gaaccaccgt
    21781 aaaccagttc gatttcttct tgtgccatgt attttcctaa cgtttgcgca ataaatttgt
    21841 aactttcttt attaccaaaa cgggaaccgc aaaaaacagt aagtttcata aaaaaacact
    21901 ccctaactca aaagtctatc ttcattctac acgtgttttt ttaaaataaa agactagatt
    21961 ttgtagtttt ttagtggtat ataggataga cgccactata tatagtattt agaaaaaaat
    22021 catttttcag ccattttata gataaaacaa aaataaataa cgtgttttca tcgatatgaa
    22081 atgacttact tatagaagat actgggattt cgtcctatat gccaataacg tgtattattt
    22141 taaacaacaa atacttgttt cttttattaa cgaaaaaatc attttgttct cgtgttatgt
    22201 ttcactgata aatgatgttt ctctaaataa aaaagccgac gaactaaacg atcaaaattt
    22261 gatcattttt gttcatcagc ttctcctaaa aatctttttg ttaacgataa atagctggat
    22321 ttgtaaaaat atcacttgaa tcgtcactgc tggaagaaaa ttcagtgata accgcacctg
    22381 ttgcgcctgc ctggaactag tgtttgatat tgggctcgaa cgtgtactga tctctagcat
    22441 gaagaataat ttcttttttt actgtataat aactttcttt cctttttgga atagtacagg
    22501 aaagtttttt tgtttccttt ccttcgacat aaggtatacc agaccctttc gacaacgaaa
    22561 ggttctcatt tttccttcat cgtcatttca aggagggtgc cgatgctctg gacagtttcg
    22621 atcgctccta aaccaaaatc tgtatattcg attctttcta actctgattc tttcatacca
    22681 ttcctgtctc aaggtacatc tattgtactt ttttcttgta atccattctg cctctcctaa
    22741 ctaattgtca aatcatccca agatatgtct tcttcctctt tttcttcttg gatgatttct
    22801 gcattcggat ctactggttt tttcaataat aaaatggtta ttcctgtgac aaaagaacca
    22861 gctaacaatg caccaatgaa tgcccatggt ctggtcatcg tagcgactgc aaacattccc
    22921 gatgccggaa tacggctatc tgcgccccaa aacatgctta atccgccacc tactgtccca
    22981 ccgactcccg ttgctatgac attgcgtgcc aagtcgttca atacgatagg gaaacatccc
    23041 tctgtgatct gacagatccc cataggaaaa gcggtcttca atgtatcgat ttcttgacga
    23101 ttgagtatat ttttacgaat gattttccct aagaaataag cgatcgtcaa gccgaatggg
    23161 gttaccatcg aagctaaaat caatacagtg attggttcat agattccttc tgaaaatcgt
    23221 gccacacccc cttatccaga ataaatggta atccagaaac agccgtgtat ctgcggtaat
    23281 aagcctaacc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
    23341 tgcagggcag ctttttcaca acctcttttt ttatttatgt ttcataatca atatggtaag
    23401 gatgcagctc cgtatggtag accgcctccg aatattcaat catttcccca atttgcgtag
    23461 acgatttccg tatccgtttg atgccgatct gtgcgtctgt tttcagaatt ccttgttctt
    23521 cttctgttaa aaatactgcc gagaaatcat cagaaaatga gtcaatagta tatccttttt
    23581 tcgtcagcaa acgatacaaa gattctttgc caaattcttc tctgctgaat tgcatcattt
    23641 cttgtggaag ataatgaaca aaataaatat aaggctgatt gttcaaataa tataagcgct
    23701 taaaacaaac agcttgctca ccgaaaaagg aaaacaaagg atcttccttc tttatatgga
    23761 ccaattgcaa atccagcgtt tctttccgga cttcatatcg ggagttctcc aagatttgcg
    23821 taaagcttgc cgctttggat aatcgattgt aaggacgatt gcttagaacc gtcgtcccac
    23881 gaccactttt ctttgcgaca tattcatctg atgccagcag ttcgattgcc tttcggacag
    23941 tgattttgct gacttgaaac agttcttcta attcactttc tgtcggcaac atcgaaccga
    24001 ctggatattt tcctgagaaa atatcttctt taattttatc tgcgacttcc aagtatagta
    24061 cacgtgtcct tttcataacg tcaccctttg ctttactatt actaaaagaa tagcacataa
    24121 aaaataaaaa aagaacacaa atcacatgtg agaagtgtgt tctttttcat tcagctgctt
    24181 taattacaag tttacgccat ttgaggcaat tacttcttta taccaatcaa aagatttttt
    24241 ctttgatcga ttcagtgtcc catttccttc atcgtctaga tccacataga taaagccgta
    24301 acgtttactc atttctcctg ttgatgcact gacaagatcg atacatcccc aaggcgtata
    24361 gcccatcaaa tctacaccgt ctgctactgc attagccatc gcttcgatat gacgacgtaa
    24421 ataatcgatc cgataatcat caataatcgt gccatcctct tccatatgat caatggctcc
    24481 aagaccgttt tcaacaatga ataatggttt ctggtaacga ttgtataatt cattcaatgc
    24541 gattctcagc ccttctggat cgatctgcca gccccattca cttgcctcaa gaaacggatt
    24601 ttttactcct cccagcaagt tcccttctga agttgctgct tgtggatcag tctcattgat
    24661 tgccgtagac atataataac taaatccgat gtagtccact ggatattctt ttagcaactg
    24721 gaagtctttt tccgtttgct ctaatttttc cggatcgact ccatattttt tatgggtacg
    24781 cgctgtgtat gcagggtatt ctccacgtac ttggacatcc gtgcagaaaa aattgaattc
    24841 ttggttttgg atcattgttg ctgcctgatt gactggattg gcatcgatgc tgtaagttgt
    24901 tgcatagata atcatgcagc cgatttggat atccgaacgc aattcgtggc cgatttttac
    24961 ggctttgcta cttgcaacga attgattatg ccatgcttgg aaaatatttt ggtactctcc
    25021 tgctccattg ctttttacta atccttgact taaagctgga aagtgaaagg ccgaattgat
    25081 ttcattgaaa gtcatccaat atttgacttt actgctgtat ctctctaata ctgtctgagc
    25141 aaatcgttca tagaagtcga tcagttttcg atttttccag ccaccgtatt cttttgccaa
    25201 atgaagaggc atttcgtaat gagaaatcgt gatcacaggt tcaatatcat attttaagca
    25261 ctcatcaatc agctgatcat aaaattctaa tcccgcttcg ttaggagtac cttcgtctcc
    25321 atttggaaaa attcgtgtcc aggcaatcga aaaacgataa catttgaatc ccatttccgc
    25381 aaacaaggca agatcttctt tgaaacgatc gtaatgatcg attcctcgat gattcgggta
    25441 aatatatttt tcttgatcaa tcgtccagtc aaattcttca ctgccgataa tcgcaaagcg
    25501 ctgcttacct ccaggcatag catctgcaac ggatagtcct tttccgtctc gatcataagc
    25561 cccttctaat tgatttggca gcagttgctc caccccataa aaatttttct gaaaaacgtg
    25621 attgcatcct gatttcctcc ttattttaca attgccagaa catcttcgtt tgcaataatc
    25681 tctttttggt ctaattcaag tatatcggca aatgcatcag tattcgtaat gacaactgga
    25741 gtgattacgc tatagccagc tgctctaatt gcttcgatgt caaaacgaat caataaatct
    25801 cctgctttga cgagatcccc ttgtttgact aagatctcaa agccattccc atttaattct
    25861 actgtatcca tcccgatatg aatcaaaatc tccgctccgt cagtcgttgt gatccctaca
    25921 gcatgtccag ttggaaaaat agtcgtgatc tctccgtctg caggtgcaac tagttctcct
    25981 acttctggat caatagcgat accttttccc atcacgccag aagaaaaaac ttggtctggt
    26041 acttcactca atggcagtac tttacccgtt aaaggacttt ttaaggtatg ccgtgctgaa
    26101 gtaatcggtt ttcctgcatt cgcatgtttg ttttctaatt gattgtcttg tgtttgttcg
    26161 tcaaatccta aaatcaatgt acctacgaat gcaagaacaa aagcaattcc tgtagctatt
    26221 actgctactg tcacatttga ctctacacca tccaccgtat taatgaaagt cggaatcgtc
    26281 aataagctga ccaaactgct ggagaaagct tttactccgg aaaatccgat aatagctcca
    26341 ccaatacctc cagaaataca agcggcaatg aatggtttct tcaaaggtaa tgtcacacca
    26401 tacacagttg gttcagtgat tccaaataac gaagtcactg tagaagaaat ccctaatgct
    26461 cgaagtttcg tatcttttgt tcgcaatgct acagctaaag cagccccacc ttgtgccaag
    26521 atggctggaa gaagcattgg catcaataca tcaaacccat attgttcgat attcaaaaac
    26581 atgatcggta cgaatcccca atgcatgccg aacatcacga atacttgcca taaacttccc
    26641 atgattagac ctgctacgat tggactaaat ccataaatcg agttgaagag accgcctaaa
    26701 gcattaccgg caaccgtccc gattggtcct aaagcaagaa atgttaatgg aaccatcact
    26761 actaaaacga caagcggaac taaaatcatt tgcagaaatt gcggaatcac tttttttacg
    26821 aatggctcta atttactttg aacccatacg gctagaatga ttggtatgac agaagaagta
    26881 taaccagatg gtgaaagaat cactgggatc ccaaagaaac tgatagcttc tcccgcacca
    26941 gcaactgctg tgatagctgg atacacaagt gccatagcaa ttgctactgc taaaaactgg
    27001 ttggctttga actttttagc tgctgtaaaa gcaagcatga caggtaagaa ggtaaagaca
    27061 ccatctgctg cagcaaacaa tatttgataa gtaccgctat cagctgtcag ccagcccatc
    27121 accgttgcca atgataagaa tcctttcaat accccggcgg ctgccatcgc ccctagaaaa
    27181 ggtgtaaata tactagagat aatatcgatc agacgattca aaatattttt ggactcttct
    27241 tttccatcct ccgaaactgc aagaccttcc tcatccacga tcgattgata aacatctgct
    27301 acattactgc caatcactac ttgatactga ccgccacttt gtacgacttg gatcacgtcc
    27361 gggtcttctt ttaacttttg cgtatctgct aaagattcat cttttaatct gaatcgcaac
    27421 cgcgtagcac aatgtactaa acttttgaca ttcttttgtc ctcctaccgc ttcccataca
    27481 cgacgaccaa ctgcttgatt atccattgtt ttttccttct ttctttttta tttaaaaaat
    27541 aaaaacccag attgaaaaaa acagtcacaa acattcgtga acctattttt tcaatccagg
    27601 ttttgcctgc catacagtaa caatccttat gattggatgc tttttttctc acttactact
    27661 ctttgaatat gaatcgttaa ataaatctgc tctgcgatcc ccatttcctg tttttgcgtt
    27721 tgtcgaagat actcattgat ccgttcgaca caatgaaacg cttttggata ctttccttga
    27781 actaatgcgt agagaaattc atcttgctcg tcagatcctt cccctttgag ataacgctgg
    27841 gcaaaaaact gcagatgagt aatgatacgc tgataattca aagattcttc atcgaacacg
    27901 atgccgaaga atttactgat gatactcagt atatctctta ccattgtggt agcggtcatc
    27961 gtcacttgca tgtcttcgtt tccttgttgg ctattcacaa aatgaaaggc aataaaaccg
    28021 gcttcatcct cagaaagttc cacacccgtc ttttgtttca cgaacaaaag agcctctttc
    28081 gctgcttgat attctttcgg ataaaatttt tttgtttcaa ataataatgg attaggcagt
    28141 tggatatttt cttttgctcg tgtgatcgca taatgaagat gatcggtcaa tgtcagataa
    28201 atattaggcg aaaattcaac ttttagttta tcttcagcca gttcgactac ttgtttgacc
    28261 cattcgatct cctctgccgg aatatcagcc atcagctgct ggaattggtt tttagcaaaa
    28321 gaatctctta aaacaaattc tttttggacc gcttctggcg aaatctcgtc cccaatcttt
    28381 ttttgaaaag ctagtccttt tcccatacaa acgacttctt ccccgaatcc atttttcgaa
    28441 atgacgacat tgttattcaa aattttatca attttcattt tgcttcgccc tctcaagaaa
    28501 aataaaaaaa cctagaattc gcctactgtt tcttagctga attctggttt tgcctgccgt
    28561 acagtaacaa tccatttctt attcaaaatc atcataccat cgaattttta tttatgcaag
    28621 ccttttcata taaaaaataa aaaaactgga acacaagtct atctagaaaa caaccaaatc
    28681 agagatgcac ctctttgcgt ttctcttttt ttcagcaaat tggtttttcc ttatcgattc
    28741 atatcctttg gctgttttta atctgtaacg acttatgtcc cagtatccta aaatgttttt
    28801 gtctctagat tgaaagcaga tctcatatca tctgtcgcat cttttatcga tgtaacactt
    28861 tccagttctc cttgtacaac tagtcgcgtg acggcgtcca tgtcgccaca agtaattagt
    28921 gtgacaattt cttttccctc gacttcattc agtaattggg tatctgtagg agcaactttt
    28981 tctttaaaaa atgttttgta tgtatacaca ttttctaaat ctgtcaaata gatcttggca
    29041 cccatctcta aattttctag cggtgtaaac agcaattcag ggttcgttgc acgatggctt
    29101 gctaaggcat aatttccttt tcccatttct tgatctggag ataatgttcc tgctccgtaa
    29161 agtagtgacg tattatccaa gcctttgaaa accggtaaat tgattgaaac tgaagggact
    29221 gcgatactag caatcactgg tagatctgtt cctctcattt gcgacctcat gaccccttct
    29281 gtcgtcattg gttctaccgc atcaaagtca aaactgacat ctttatcgtt attctttttc
    29341 agatcttctt ttgttacatt cgctatagca tatttatctc ctgtctctct gacaaaataa
    29401 tccttgatct gttcattgaa aatcaaagct agtcctacta ataacaagag gaacaagagg
    29461 atattgacta gccatctttt tgcttttcct ttttttttca ttttctctcc ctccagactc
    29521 attttactgt ttttcttttg ctctttccaa cagccgaatg acacatttct acaactctgt
    29581 gtattttttt cgtacacctg cgcttttttc tacaggatat ttcaaattat ttttgatcaa
    29641 tttttcttca atgatctcat ctagatccag gtgaaggttg tctgccatca tgtatgaata
    29701 gatcaataca tctgccagtt cttctttgat tcgttctaat tgtgtttctt ttacttcttc
    29761 cggcgttttc cattgaaaaa gttctaacaa ttcagatgct tccaaagaaa tagagatagc
    29821 caaatctttt tcgttatgaa attgtcgcca attacgttca tctctaaact ggttgacttt
    29881 ttccatacta ttcatttgct tgccttcttc caatcacttc ttacttgatt aatcatacca
    29941 tttttttaca taaaaaatag atgatgcttt taaaaaaact cctgttttct tttatttaaa
    30001 ataaatgtca tagaaaatca ataatttagg tagccaccat gtgtatgcag tagcgtgtct
    30061 tcttcccttg aggctttttg tttttcgata cgttcttctt cccaaacaga caacgctact
    30121 agaatctctc ttaatgattt tccttcttcg gtgagcgagt actctacttt aggcgggact
    30181 tggttatagg cttttcgatg gatactttca tcttgttcta actctcttaa ctgctgggtc
    30241 agcatttttt ttgtgatcgt agggatttgc ttgcttctcc acgcctaaaa taagcgatat
    30301 tgctcttccc tttatctggt gtaagcaaag cagttagcat tttgacgatc gtgggttccg
    30361 cttttcactt cttatctact tagtatcatg aagtatactt tcgtactaat actaagtagc
    30421 ggaagtgtca actactttgt ttaactacat agcaaaagaa acagaagggt gacaacggtc
    30481 atcacgcttc tgtttctttt gaatatgctg tgttgttgtc tctatctccg tgtagacttt
    30541 cctcaaactt atcctttgac agtaagtcag taggctgatt tgctctttct agatgccaga
    30601 tacatttcta caaaaaaata gtgattctgt tttgaaaatt cgatttttag ttctgcattt
    30661 tcgtaaacat ccaacatttt tttcgcactg tatagaccta acccgccatt ttttttagag
    30721 gtttttcctc ttttgaccat ctccgatagc gtcaaattcg ttggatttgc cttgttcgct
    30781 atactaaaac gtgatccctc tttttcttcc agcagattga tcgtaatcgg actctgatcg
    30841 gctacacgat gatcgaatgc gttagaaata aaaatagata aacagcgaat aaaaacaacc
    30901 agatcaatcg ggattgtttt cacttcgaca ggaataacta cttggatcgg gattttctcc
    30961 gattctgctt tttctagaaa ttttaaaaag acacccctga cagctgggga tacaatattt
    31021 gttagctgtt gtatttgcgg tttttcaaaa atagctgcag aatgatcttc taatatttct
    31081 aaaaattcca acgtttcttt tttattttcc tgaagagtaa tttttaatga aaacagtaga
    31141 cttttataat catgtttgaa ttcctctaga tgatggtaat attcttcatt tatttgatac
    31201 agttgaagcg attcctgatg ttgtctttct tttacttgga aactgatcaa cataatggaa
    31261 aaaaacaaag aaaaaaaact gatgactatc gacaaaagag atagaagcag ataattagat
    31321 gagcggaagt gaacacttcc caataccaag taaatcacgc cccagcttaa taaaatacaa
    31381 atacctccaa aatagtgttc agttggataa gtaaaaagca agttgagaaa tctcgtctgt
    31441 ttgtctatac tttttactaa tagatagaaa ataaatgcga taagtagtgc aaaaaaactt
    31501 gctgttccaa gatgtctctt tcctaaataa tccatcagat caaaaaccag aaaaaagctg
    31561 agtccagtga tagcaaaaaa aagattactg atcaaacagc tgataaacag gtttttcgta
    31621 gtaaaataaa aaagaaacat caataataag ccaactaggt aaggaaagct cactttaggg
    31681 ataggaagta aaagtgtcac caccatcaaa gctatacaac caaagctgtt ttttactagc
    31741 agttgtttcg aaaagaataa atagatacct aaatctaaaa tcaaagtaaa ggtcaaataa
    31801 agtgtcactg ttgcgaccat cttattcctc cattgttttg tttcaaaatg tacacatatt
    31861 tttttaatag gagcctgcat atgaaaaagt attttatcat agaagataac cagtttcaca
    31921 gcgaatggat catcagagaa ttggtcaatc atcatatcga gaatatcgtg cagctcactt
    31981 ctatctctca aatcaatcag ttgatccagt tgggcactat tgataatcac gatgttttct
    32041 ttatcgatat cgacttgaag caatcactca caggtattga tgtcgccgaa caaatacgta
    32101 aaaaaaatgc acatagtaat attgtttttt tcaccgctta tacgaatttt gcagtagaga
    32161 tcatcaatcg gaaaatcctt ccgattggat atatcagaaa agacaggaat ttatctgaac
    32221 aaattcgtca agtgctagaa cagatcaatt ggaaagaaca gcagatcgta aatgtcgata
    32281 aaatctttat tgatcaaaaa ggtggaagtc tcgtcctgta tcctcgggaa attttatata
    32341 ttgaaagtat caaaggtttg aaaaaccaag ttatggtcaa aacaactaca gaagataaat
    32401 tagtgaatac ttctttaaaa caattgaaga cagagttaga aaagtatgct tactttttac
    32461 ttttaaaatc ttactgtatc aacttatcgc aattgcagaa aattgattct gctcattatg
    32521 tgttagaatt tttcaacggt gactccttac tcgttggtcg aaagatcttt gaaaagacaa
    32581 aggaacgttt ccatgacttc caaaaaactg cttcgagttg atgaataaga gctgagacgt
    32641 aagcatagca gatttcaggg agtgggcctt gaatcggctc cactccctga aatcgttgat
    32701 atgattttac gcttcctctt ctgtaggagc atctgttaac gaccatgtga atgttgaaac
    32761 gtagttgtct acagcataat tggatacagg aacaaataat tccactgatt gtctcccttg
    32821 ttccacattc tctcctaatg aataaatcca tgtgccaatc ccttcatttg ctcctgcagt
    32881 tactagatct actgcctgat ctgctgggat ttcgattcct tcagcgccag ttccagaaac
    32941 ggtagatggc attccactgc tattagttgc gctatttcga atcacggcgt ttttgaattt
    33001 cacctttgaa ccagttagtt tattcgtatc agatgttgta ccaataaatt ctgttcgttt
    33061 gactgataat ttccaaccgg cataatttcc gcttttatcc gttacttgta cgaaattctg
    33121 tacttgtgtt tgtgctccag tactgtccgt tccgctttgt aatgcagcat atactgtttt
    33181 tgctgtacct aacttgattg ttccaaaatc aagatctgga gcttggtcaa tcgtcagtaa
    33241 tccggatgtt ccctgatttg gttcgtcagg tgtaaaagga gtatcaggat catttggatc
    33301 aacaggagga actggaacaa cgtcttcagg atttgcagca tctggtttgg tcacagaaat
    33361 cgttccttct gtagtggctg agctcttatt ctcagcaaaa acactttgtg tacataataa
    33421 tagacttcca gctagaacgg tacttaatgc aattgttttt ttcatttctt tttccttctt
    33481 tcttttaaaa taaataatcc agcaaataag ccaataagaa tgactccaac aagtatcagc
    33541 catcgccagt caaatgtttc tggctgattt tttaaatcaa tatccttttc attcaattgg
    33601 tccgctgcat catcagttat ttcaaaatcg gtatggagat tccagcgttt ttctttccaa
    33661 gcacccttca caaccgcttg ataatgtccc ggagctaatt tttttccttc taatgggaca
    33721 agtaaatcaa aatttgtatt gggtgctaca tctatttttt ctttttttat tgaaaggtaa
    33781 ggctcttttg tttctttttt gtagatttca accgatgtag tcaagccgaa catataagaa
    33841 gcatttggat tttgaagatt cgccttaatc acatttcttc catcgctttg atccgcataa
    33901 gcttttttta atttcaaatc tggggaagac tctttttcgc tatttcaaat caagattgct
    33961 ttcacttgag aaaactgatt gacgacacca actgtacttt tgttagaaac gttctctttt
    34021 ttttcttgaa acgtgatccc tcctgcgacc acaccagcca gctctttttc tggcgctttc
    34081 agtgaaagtg tctcagtgac cgcactttga ggaggaacag ttatttctcc cactgttttt
    34141 gctaagtttt tcatatcata acttaatgtt tcatcaaatt tactttttga ttctccatat
    34201 tcgatcacac cgtttgaatt cgtcgtagcg gagtggatca atgtctcaac tgttactgat
    34261 ttatctgttc ggttaaagac cgtgtagggg atttcctgtg ttttcccagg ctccatcctt
    34321 acatttatat atgttttgtt cttatcaatc tgattagccg gtaattcaaa atctaccgca
    34381 aaattgaatt cggaggcatg gacaggtgtc tgccaaagga tgaatccaag gagaaggatc
    34441 ataagtaaag agcagttgtt tcgatgactt ttattccata ttcgattcat caatcatcct
    34501 ccttgatctt tattctgtcg gcgcatctgc taatgtccat gttatctctg ctgagtaatt
    34561 accagctatc tgattttctt tggttaagac aagtttaaac gcccgaccgt tatttaaata
    34621 ttctgaataa tctgcttgca cattcgttga attttgtcct ctgacaattt ccagttcttc
    34681 ttcattcact aattgatact cctctccttt cttgaaatat aggacatcct gcatagaaaa
    34741 tcctgcatca tttgtgaaag gctgagataa ttttatcgtt aagatccacg gatttttcgt
    34801 ttctcgctga tcataaatcc ataatttttc ctgtatatcc atttggccaa atgcttcttg
    34861 tgtcaaacca gttgctttga tctgaccaaa atcgagtttg gaaggggcaa gaaatgagag
    34921 accaccttct actacgatct tcgtagcagg tgcaaaaggc ggggcatcat gataaacaac
    34981 tggcgtatca cttggattgt gattcccaat ttcatcgttg gtacttggct tgttctgcac
    35041 acccttagca gtagcttctc ctgcttggtc gtttaataca atttctaata catctcctgc
    35101 aaaaagttga ttttctggga tagaaagaac aaattcccct gtatcatcaa tagttgtttc
    35161 tgacaaaaca gcttgatttt ttttcaatga aaccaaggct cctgcttcac ctgtacctgt
    35221 gatcgtgcga gaagctgcat tgatcaatgt ggtacttaaa ttagcaggtt caggaggaat
    35281 cacatcgact gtcgttttgg cagagtccag ttctgtcact tcttggccac ctcttacttt
    35341 tttagcagca acaacttgca cttgatcctc cgtttttaat ctctcatcat tgggtacttg
    35401 aacttcgaac aagccgcctt gttcgccttc tccccatggg ttatatcctg ccccttcatt
    35461 ttcatctgtt tgtccttgcg tgagagcagt aatttctaca gatgatttcg tcgtatctgc
    35521 gtaagtaata cgtaaagtca cttctacttc gcctgtccaa gcatctcttg gctttgcctc
    35581 gacaccttcg ggaacaacga catgcccata gattttttta tctgcattcg ttggcactct
    35641 taaggattca atcatcggga gctgattatt ggcactgatt cgattataat ttttcattcc
    35701 ggcacctgtc tgagcaaatt catttgttaa acctgcacta gggcttgata cgaaattgcc
    35761 taagtcggtc ccattgatcg ataagtctgt catatcagaa aaataatccg ggtctttttc
    35821 aaagttatcg aatgttgcgc ctgtttttcg ccataaagcg atttctgaat gggcgatctt
    35881 taaagaagaa tctccatttc cttggaatac ccagccgcct atgccattgg tctcgcttcc
    35941 aatccgtttg ttttggaaat caaaataggt aggagattct aagtccacat tcaacacatc
    36001 cccactgatg atgccggtct tatcttcccc tgttttcccg accatgacta aataagtttt
    36061 ttcacctgcc gtaaaattta aatcaatatc tccagttgta tctacagttg ctccggaatc
    36121 tgcgcgtata tcgatatttg atttttctcc agtgactgta aaatcagctg aacctggact
    36181 ttctagaaaa ccagaagcgt cataaaattg gatcgcttga tttccttgat tgagtctgtt
    36241 cacgtaagat ttgccatcac ctgtatttct taccctcaat gagcctccat tggttactgt
    36301 cagcttttgc gtaccattct caaaacgaac tgcgctggaa cgcccactct gcttatcgat
    36361 gtcgataact gatccggctc cttctacgat caaagaaagg ccttttccta caaaacgcat
    36421 cccattgttc ccgggttgat cgtcatctcc agccattttg accgagattt gtccaccact
    36481 tgatacatgg atttgactag aatctccttg caagcgaaat ccgcccgtag ttgctgtatt
    36541 gatggtgatt ttcccaccat tcgatacagt gactttcgat cctgaacctc tcatttggat
    36601 gattccgcga ttgttgtcat tggtaatatt gatctttgta tttaccgaaa attcgcttcc
    36661 cgaaccactg acttcaaaca tgacattacc aggtagcgta tcgactgcct ctccttcatc
    36721 gctaacgcca tctgcccgat cataatcaag agagaccttt gaaccgctta agatatggat
    36781 tcctggcgct gtttttccac taccagtctc atctcctgaa tctagaaaac cgtagaattt
    36841 ccctagatac ggtccataaa gttgactacc attgatcaca ttcaattgat agcttctaca
    36901 aaaataggct ttacccggag cggcattcag tccttccttc actggttgga catccaacac
    36961 accggaaaca ccatctaatg tgacagttgc acgaggagcg taaatcagtc ctaatttgga
    37021 cccatcatac aaatcaagtg tcccggtaaa ttttatttct cccgtaaaat gatatctgct
    37081 tccccaaaaa tcggtcatat aagcaatgat aatggcattt ccatcgatat ttggtgtcaa
    37141 tccacctgga tgtgcagctg ttcctttaaa attctggaac tcgatcgact gaggtatctc
    37201 attgatataa atatggtgtg tgccgaaatt gtgagcgaat ccaccaaaat caataacgat
    37261 atctcgtttg atctctaatt ccctgtctaa agtcaaaggc tgaatcagtc ggatcttagt
    37321 tactgattcg tctgcgagaa gctgcgccag ttcttctgtt gtggcagctt cgctgtaccc
    37381 atccgctgaa acagctcttg ccagtgccgc ttcactgcta tcagacgttt gttcagctac
    37441 ttctgaggtc tcacttgtct tattttcttc ttgcctttta ttagtagaag cctcactttg
    37501 agattctgta gtttcagaag taatcgattc ttctgaaaaa gtagtcttgt caaaactttg
    37561 attctctgat tgcgttgatt cgtttgtttt ctctacatcg gcaaaaacgg atgtcaccat
    37621 caagagaggg aagacaaaca aaatcgacgc aatcagcttt tttacacatt gattcattct
    37681 ccaccatcct ctctgacgtt ttgacaattt tattttatcg ttttaatcta tttccaattg
    37741 tactttcata tttaaaatat gtatttttat ggaaaaattt ttctattagg tgtaaatttc
    37801 ttttttgtac aaaaaaaagg ctgtgacaaa agtcttgtca cagtccctta tcccgaacaa
    37861 gcggtgttct cccgccaact cctcggtcac aacctccatt ttaacaataa acctgtaaat
    37921 gtgagggcaa tactttgatt gtcacaggta atttatcccc ttcatctcca tcaacattcg
    37981 tcgtcaattc ttcctcatca gctaatgaca caactaattg ctcacaagta aggtaggcta
    38041 gatttttcgt agattcatct actcctttta ataggtctgg caatgtttta agcgtatcga
    38101 tcaaagaaga atcttttaaa taaaccaaat ggagcttacc atcatccact tttgcatttg
    38161 gaagtaatgt ttcaaaaccg ccgactgagt tagttagtcc tatcagcaag gtactgctct
    38221 caatttcttc cttctttcca tcaacttcta cgtgaaacga gtaggattgt gtggaagcta
    38281 actgtttgat tcctgacatg aaataagcta gttttccaaa tttcgtcttc ttctccgcat
    38341 ctacatcatt gattgattca gggatagagc caattgcgac gacattcata aaatagtcgt
    38401 catttatttt tccaatatcc aatggcttga ctgattcaat cgaaaaatga ttgatcgctt
    38461 cctcaggttc tagcggtatc cctaatgctc gagccaagtc attcaccgta cccaatggga
    38521 aaaatccgaa atttggccga tgttcttgtt cagcgattcc actgattcct tcgttgactg
    38581 ttccatctcc gcccatgaca aacacactat gatacccttc cgtagccgct tcgcgagtaa
    38641 agttttttgc atcccctgct ttttttgtat gcaagacgac cacttcatca aacaccgatt
    38701 ccaattttgc tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct gaactggggt
    38761 ttaccactaa taaaactttt ttcatcactt tctttccctt ctgattgatt tctttaatat
    38821 aacgatagca taaagaaaaa aattaggctg tgatgaagta ctgttcatga aacattcatt
    38881 tattttttga caagataaaa catccatact atgaaactga ggaaaattaa aacaatgata
    38941 taggaaataa aagaaaatga taggcattgt atacctaata aagtaaaaat cttaggtggc
    39001 agataaaaac acaaaaatag caagagaatg taatagcgtt tcttccactt tttgttatca
    39061 gaccttatag cgatcagagg aataccaaat gcataactga aaaactcttg atgtactgtt
    39121 aatatgtcac ggccttgaat aataaatgcg acaatccccg ctataattag tgtgcctaac
    39181 accaaaacgg aaaattgatg aatatatttt atatacaaat gatccattgt acttcctgcc
    39241 tttttatttg tgagaaaaac aaatccgtat acgtgaataa cttctagaaa taaaagaaat
    39301 ttgcaaaacc actccattta tctacttcta cgtgcatttt ttgatattgt tttctttatt
    39361 taagattata cagaaaaata tctagggtcg ctggattcta agtaaaaaaa atttttaaaa
    39421 taaaaagaac taaatcaagt agtaattaaa ctactgttga tttagttcta ataaaaagat
    39481 ttagatgaac agttttattg atttaaatag ttcatatttt ttatttatac cgcttattta
    39541 ctacttgctg tactagaatc tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct
    39601 ttgattttta cattagcgtc tttcaatact tcgccaatta ctttattctg gaaggtactg
    39661 tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt atccatgtcg
    39721 ttgcctttgt tttggttttt caccatttta accacataat attccgtagt ataagttgaa
    39781 gcattggtag aagtgatcac atcagaaact tgaccatctt tcaacttaaa ggctgcttct
    39841 ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc gccatcttct
    39901 tttgtcgtag tatcagttga cttgtctttg gctaatttag agaaatcgtc tcccttatca
    39961 gcagctttct tcacatcttt tgcatcatct tcacttgcta cttggataat ttgagcttcc
    40021 acttctggat ggaaagattc ccaagccgtt tttaaatctt catcggtcaa tttgatatgt
    40081 ttctttagac cttcttgaaa ggctagactt tgtttgatac tgtctttgaa tgtttgttct
    40141 gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac ccaattgttt ctttgtttga
    40201 tcatattgag aatcgatttc tttggttgat acgtcatctc catatttatc ttcaaatact
    40261 tgataaacga tcaactgttg cacagcggaa tctgctcctt gcattttttt caagctatcc
    40321 attacttctt gttcgctgat tgtaccgcct ttataactaa cggctgtttt ttcaccggca
    40381 ttcgtacagc ctgacaataa gccagtgcct agtacgatgg caagtgctaa aaatcttttt
    40441 ttcatttatc aattctccta cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca
    40501 agatgaaaat catcaagaac acctttacta atctgctcga tacgtcctga atagatataa
    40561 ggctacacaa tccagaaagt tcgctcagca gaataaatgt caagtagcac atcacacctt
    40621 aatatactac ttcttttgtt accgtttttc aagtctatct tgcgttccgt atttaacgta
    40681 actaattctt agaacaaatg ttatcgattt tgtttttctt cttccgctgc tgggatattc
    40741 aaatcagaca cttttatgtt cgattttcca gacatttttt tgttgttttt attatgatat
    40801 atttttatac aactaaaaat gaatatatta aaactaccaa aaataacttt taacgctgtt
    40861 tcattatatg ttttttctat tttatataaa cttttggata cttaatacca accggttgga
    40921 ttgtaccaac atgagaatgt gtcagttcta tttcctgtcg cacttgcata ggcccatact
    40981 ccttttgttg catttgctga tcgaatagag tttccaactt tcgcttttgc accgtgacga
    41041 ttatagacac gactatagtg gttagaatga cctgtttttc tcgtacaagt tgtatcacta
    41101 taataaaccc atcttccaga gctatgatta attgctgttc tagaaatttc ccggtctact
    41161 ggtaaatttg acttaataat attaccttta gcatcaaaat gaatctctcc tggctttaaa
    41221 gatttgtaat agatttcacc gtgaactgaa tttgcttcca cttcagatga catagcgttt
    41281 cctccaacaa aacctatccc tagtgcacaa ataataactg cttttttcat aaaacgacat
    41341 cctttccttt ttgattttta aagcatgatc ttcttgtttc ctattttggt cagatatgga
    41401 tcatgcgtga ctacgataac taacgtacct tgggaagctt gttgcgataa gatttcaact
    41461 actttatcct tatttctttt atccaatgac ccagtcggtt catcggctaa aatgattttg
    41521 ctattcttca ataagactct cgcacaggcg attctttgct tctcaccacc agataatgtg
    41581 tagataggcg tacttagctt cttgtttata tttacttttt tcaatgcctc atactttgct
    41641 gttcgcttat ctgaaacatt taaattcgaa aacttcaatc caatatctaa attaaaatcg
    41701 attgtttcat attcaatcaa cccaaaatct tgaaaaagaa aagatatggt atttcttttt
    41761 atcaagcgct tctctctccc agttaattgt ttcgtctctt gatcgaacaa atagaattgt
    41821 cctgaactga actgatcatt caatcctaat atattcaata acgtactttt acctgctcca
    41881 ctttctccaa caacagtcac aagatcccca gcatccgcag tgaaacgatc caaatgaaga
    41941 atcgttttgt ttcgaatgat ttttttcgca ccttttatca ctatcaacgg ctccaattgt
    42001 taacctccat tcatcagcaa taactgactt ttcttttctg tagacaaaat aaaaacaact
    42061 gaaagaataa aatcaatgaa gctaaagaaa aaaatattaa aaagatattt aggctctctt
    42121 tctataagaa aaaggaagat tacacttgtc gttccccatt tgatgaggtt catcatgaag
    42181 aaatctttat acgtttcaaa gagcgaataa ccacttgaac gaaagatagc gattttccga
    42241 caattgtaga caaaatatat gcataacatg acatagctca acaagaagaa agaaaagaaa
    42301 gtgagtaaaa atatctgtat caagtaatga aatgatctgc ttaacacttt gacagctatc
    42361 tcttcagtct gagtatacgg aaatgataac tgcaagttat cacgtaactg actttcttca
    42421 atcagctcaa ataacctttc tttcgacaaa gagcctatct tgatctttaa cggtggatag
    42481 atttctccgc tgagaatatt tctttccaaa tacatactgt ttttcaatgt cagtatttca
    42541 acaaccggat aatgttcgat ccaggggata gaaggaataa atgtataaat gggttgattg
    42601 ttttttataa atagaaattt cggttctttt tcactgattt cttgataata ttctgttatt
    42661 tgcgccaggt cattttttaa atctgtatgt ctatatcgtt cagggatcaa tataacaggg
    42721 tgcgtttctt tttcggttat ttggatcaac cttccagaga catcaatcaa aggaaatttc
    42781 ttaagataat tcagatttat ttttatccct ctgccaaact gttcattttc ttcattcaag
    42841 taatctgata cattgactaa tatacttccg tttttattca aagtttcata taattcctga
    42901 tctgctgctg atgcaaaacg attggaataa ataaaatcca cgtggttttt cccgatggtt
    42961 actggataaa atacatgata gtcctcacca gcgatttctt ttggtggttc atgggaaaca
    43021 gctaactgtg aagaagaaaa aacgatagtt gctaattcga cattggcaac ggtcaaaaga
    43081 agagaaaaca acaatccaaa tgtaaaaaga atgtattgga tatagttgaa cgcttctctt
    43141 aacgaagcga ttttttcaac taataaaaga gaaatcaaag agataccata tattccgctg
    43201 aacaagaaaa cgtacatctg gaacaaataa agaaattgct ccttataata cataaaaata
    43261 aaaccaccaa tgattcctgc aattatgaat gtaataatga ttttcttttt ccccaataaa
    43321 caccacaata cttgatgtaa tgaaaagtca ttattcaaca ataaactgat ttctttattc
    43381 atcaataaga aaaaggatga taacaaaagg aataaaagaa tcaatccaat agttaaatac
    43441 actcgataat tatatacgtc tgtatttgaa ataaccgaat acgaatagct attttcaaag
    43501 tcttcaaaaa tattagtggc ataatcagtg tgaaatgctt gattgaatcg atcagtgaat
    43561 agtgaaatga accgttcata ggcttctttt tctctcgtca tcaagaaaaa ttcaccttca
    43621 aaatctttta ttttgatcga ttcttcaatg gacgttacgc gaaaggaggt attagaatag
    43681 ggtaccttct cgtgagaaga tttgacatcc ccttgataat acaatgtcat ttcaagcttt
    43741 ggctgtatct tgaagttcca agatgtatct acttcattgg attcatttac tgtttttttc
    43801 atataagaaa gctcagtttc tgatgagaca ttttcaaata ccatgattag ctgctcatat
    43861 tcttcttgcc caataaaatg ttgtggcaat cgaatcggag tataagtatc tgtttgatgg
    43921 aaaaattcat ttgtccgata gagtacctgt ttatttatac taaccaaggc aaaaccactc
    43981 atgataccgg tcactatgat tgtgagtaaa actgtgaact tttttttgat tatcaccact
    44041 ctcccttact taaatgattc tttgataaaa tcataccgtt tttcatcaaa aaaacgtatc
    44101 tatcaagtgt ataggtcatg tagaataaat ctatatactg tagaatcgaa aggagaagaa
    44161 gatgaacacc ggagaaaaaa tacgaactta ccgcagaaat tgtaagctta cgcaaaaaga
    44221 attaggcgaa aaaattggtg tttccgataa aacgatttct agttgggaaa attctcgtac
    44281 aatgccagac cttgaaatgc tgtcactttt acatcatact ttaggtctgc cgattgattt
    44341 tccttctatt gaatcagcta ccgaatcaat agaaccaaca ttgccagatg aacctcttgt
    44401 taacaagaaa tcttattcat caaaacaacc cttctcactc aaaaagacac tatctttctt
    44461 tctgctcgtc ttttgtttgg tctatctttt tcttatcaat tatcaataca aaaatgaaac
    44521 gatcgatcga ttcaatcctt ttttgaaacc acaaatcggc tataccacac tccctgtagg
    44581 atacgtggac aattccatcg aacgttgggt atgtaatgat atctttggcc atggtacatg
    44641 gctgtctttt cacgctggag aggtagaaaa agagaaaaac catgctgtaa tcaaacacaa
    44701 aggaaaacac gtcatgaacg ttcgcctgct cacttgggaa gccattccct cagattacca
    44761 agaaatcctc agccaagact atgacgacgc cttaaaaaga agccctcagg agccagatag
    44821 gcattaagta ggagatgaac gtagagatag aaaaaatcaa tgcacatgat cttatccaaa
    44881 aaacaggggt acaaattaat tgaaaaagcc ttgagatgaa agttcctaaa taaaaaagtt
    44941 cagaatgata ggtatttctg gcttatagta taatatttac gccacagcct cccaatgatt
    45001 ctttttggaa tcataatcaa ggatggacat tcacaaaaat attttgctca ttcgatccga
    45061 atagattaac gaccaatcta gtaagtaata tagattggta cgtagtcaag ctgcacataa
    45121 tttagatcaa ttttagttct tttttatatt tctatgatct gctcatttac ttttatatga
    45181 tggcttacag taacataaat aaatatgata atcgctggta gtttatccaa gagtactcaa
    45241 taaaatacaa aatggattgt tcaagcgcct atcataatga taaaatgaaa caaataaacg
    45301 atacaaggag gaagagactt tgagtaatgg cagaacaatt ttttcattgt aaaaattgtt
    45361 cagttgttaa tctatgggat ttcggtagca tgggcatttt tttatagagg aatcacacca
    45421 acctggccgt tttatctctt gttagtggga atattagtag gaaattttgc ccctcttcac
    45481 tatcgtacga acttttttcc aactgattct ggcctttttc ttaaaacaaa gacgcgaaaa
    45541 ttagaaaaca ttattgaaat gacaattacc tgtataatca ttttcttagt tatattccta
    45601 aacgtcttct aataagaatc tcttaaaatt agcttgttac agttagttac ctagtttata
    45661 taacacagtt atcttatctt ccttatcaac atcttttgcc aactctttgt cttaaattcg
    45721 aaacatataa gcagagctac gaacaccaag actgtttcgg ctctgttttt tgcatcaaat
    45781 tcactcttat atagctgata atctcggctc tttaaaagaa attattttcg cttcatttta
    45841 ttataaaaag gctaggaacc ttgatgttat caggttccta gcctttattt aagtattacc
    45901 ccacagaccc ttccatatag atttttaaat tacacactag tttaaactaa aacaaacaag
    45961 gaaaaactta ccatttaatc aaaaaattag ctcatatgaa tatattagaa tgtaaattag
    46021 actggtcaaa atctggtcaa aaaagaggac gaatacaggt agtcctcttt tttgttattt
    46081 tgatattcta aatcattttt tattggatta ttcccttgtt cgatatagac ccaaattgat
    46141 ctatcaataa ttatcaaact gagtcatata gagacaaaaa tttcaaaaat gacctgttaa
    46201 atggtcatcg aatatcattt aaaatcaata cgacggagtc agacacggag tcaaaacaag
    46261 cccgctgaga gtaaaaaata tacagtccca taggtaaagt gccacaggac tgtatatttt
    46321 tttatttgag attacctgaa atgattccat ttaattttta tctcattttt tcctactagt
    46381 tagtatcaaa tactctccta ttttctcaat aaactatttt gggttacatg gtatctgaaa
    46441 cagctaaaac cttgtcatta tagcaaaaaa tcactttatc tccgtttcat ttcagataca
    46501 tttcacttaa gcggattcca aaaaaacaaa agaaaaagga acccctagtt agcctatgct
    46561 ttttattggt tcctctcaaa acacggtcat ataacaacgg ggtggggtaa atgaatgaaa
    46621 aaggtggagg aaacattatt caatagctgt cttaaatact tcgttataaa atgtgaacaa
    46681 ataaaagaca tctatcccta ttttctacac ttccacttga ctaccatctt tgaatcgtac
    46741 aattttctta actcttctcg tttacctcaa ttcattctca agtggaatca actcatcatc
    46801 taacatttta cgccatgctc gcttcatttc ttgtgggtat ttaaatggct tcgcttctgg
    46861 atgtttggct ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat
    46921 tggatcttca cctggtagat attgcagatt agaagcatgt aattctttct cagaaacaaa
    46981 ccactcattg gaaaagacat gaatcaaatc atcactcgct tgtgtaaaat agcgacgttt
    47041 aatgacaaag atatcttcat tactatctaa agtccccaca tcaaatgcat ttaaaatttg
    47101 tcggtaaact tctttgactc cttctgtttt attcaattta gccaaagctt tttcaatttc
    47161 ttctcgtgca tctgaactat tcgcagcata agttcctagt aattgatcaa tataagttga
    47221 atcaatatca tacaacttag tggtattttc actataaaat tcgatcgttg aaaaatcttc
    47281 tccttctttt tctggttctt cctctattaa tgaacccttc acggtctcgt acacgccaat
    47341 ttgttcttcc aaaagttgaa tttgattgtt caaagttgga gatttcacca tgtcatcatt
    47401 gtaatcgtca taagtcacta acgattcata agcacgattc aattcttgaa atgctttgac
    47461 gtactcgata cggatttcaa gtggcgtatg ttcatcaatg gattccatgg atgaaacctc
    47521 ttttaactta ccatatgctt gtttgaatct ttttttggat tcctgataac ttggataaat
    47581 caaacctgat tcttcttttg cttttgaata cagttttgtc gcatcctcga cattcttctc
    47641 cattgtcgct ggcttacgaa acgtcacaat caatcctttt gtcttatcag gatacgtacg
    47701 attcgtacgt gaaaaagctt ggatcaatcc tgcatactct agattacgat ccacaaatag
    47761 ggtttgaatc gtaggggcgt caaaacctgt taatagacga tccactacaa tcgctaaatc
    47821 gatttgtttc ccaaattgtt tgaattccgc tctttttcgt gccaaacgat tattgatgtc
    47881 tccgttataa cgctcaatgt ctgctaatga ccaggaagta tcataatact gattatattc
    47941 ctcaataatt ttttgcattt cttcttgttg ggcacttgaa tctcggctat tttcttctaa
    48001 tgaataggta atagcgattc gtggaaaatc aggatcctcc atcgttcgtc cttcacgaat
    48061 gagctgattg ggaaactctt ttgttaacca atcaggctct ttcgtcatct ctttgatggc
    48121 ccgataatac tttttggcca tgtcaatgga actcgtggtt aagattgcag atttcgttgg
    48181 cctgccattt cgaaaatcaa attttgtata ggcattatct ggacggaaca ttttatgaat
    48241 gactttttgg atatgttcat ctgcttcaaa aacagctggg tccaaataac tttctttttt
    48301 gacaggttcc atttgatcaa tcatacgatt gatctgttct gatgaatact ctgcatatgt
    48361 ttctacttct tttaatttcc gatagatttt attctctagt gaagtcggct ccacggtgtt
    48421 ttcatgctct acttgaaatc ctaagacaga gccatcttct agcgcattct taatggtata
    48481 ggtatgcaag acctctccat actgatcatg ggttgtacgt gccaattgcc ctttggcttg
    48541 tttccgattc tcaggaaaaa tcggtgtacc tgtaaagcca aaccaagtgg atttcggaaa
    48601 catttttttg atttcttcca tattttcagc acttaacgct cgatgacact catctactac
    48661 aaacacgata tgttggccca ttaatttttg gaagcgattg gttccttttt tctcttcttg
    48721 tttctttgca tatttcaacg ccgcatctag tttttgacgg gttgtaatga ttacaacatt
    48781 tgcattggca tctgccaaca gggtttcact taattctttg gcacttcccg ttccaacgat
    48841 caagctattc gctttggcgt tcccggaaga aatcccagta ttaaattccg aagcaaattt
    48901 tgtaaactct gtggtggttt gattatctaa atctttgcgg tccactaaca tgatcgttcg
    48961 atcgatcccc gattttcgtg ctaaaagctt agtagataca aaactagtca acgtctttcc
    49021 tgaaccagtc gcatgccaca cataccctga ttgatgttta tttgcggcaa taaacaaggc
    49081 ttgaatcgca tgtacttgat aaggatgtaa caccatgagg gttttattgt cttgatcttc
    49141 actgacgatt gtataatccg caatcaaacg atgagcatcc gggatattta acacttgttt
    49201 acaaaactca tataagtttt ctactttttc attgtctttc gttcgccaac taaataaaaa
    49261 cttagggtgc aagtctttcg gtaaggcatt tgcaaaatac cgagtggttt gttcattgga
    49321 aaccacaaac aactgtaagg tcgaaaagat attgttccga aacatacctt cttctgcata
    49381 ttttttgatt tggttaaatg cctgataaac accatctttt gcagttactt gtttcagctc
    49441 gatttggaca atcggtaatc cattgattaa taaggtgacg tcaaaacgtc gatcccgtgc
    49501 ttctatgtta cttccacgtt ttgcaatttg atggaccact tcataagtag agattcctcc
    49561 accaatgtct tgattggaat aaaggataag tgagacagaa cccaattgcg aatcttcccg
    49621 ttcaatcgtg atccgagcca tcccattttc accttttaac catttcgcag catcaaatgg
    49681 ggttttcgtt cgcagcagta attccgtttt gatcgtttca aattctttat cggtcaaagg
    49741 aaaatcattc agttctgctt gattatttga gataattttt tggcgtaaat tgacccaaag
    49801 gtcttcttct gattttagat ccggacgata cgtccactga ttatgccctt ctccaagtac
    49861 acgaatcaac tgttcttcga cttctgcttc ctcacggtga ctaattttca ttcttcttcc
    49921 ctcctagaca aacatctttt gtaagaagct ttttttcgtt tcttttagta aatctaactt
    49981 acgttgatga agagcgatcg tatcatctag ctgtttgaag aaagtaccga ttttttgttg
    50041 ttcatccttt acaggaacct taacactgat attctcaata tcagaactgt tataagaagg
    50101 caatgcccct acttgggagt atcttggcaa atttatagtt tcaaacaata ttttaccaaa
    50161 atagatatcc caagtattat taaataaata agccatggta ttgttatcta ttaagaaagg
    50221 gcttgtgacc aaacgttttc tattaagcat tattgctgcg ccaactttag caaatataat
    50281 cgctggcaca tcttttattg gcttccattt tttacgctca atttgctcat tagatacata
    50341 atgatttgca tttctcattt catactcatt accaatgtta ttcatgtcgg aaactttaaa
    50401 aaatggagta ccttcagaac caccttgctc ggcatcagga aatccaattc ccgattgagt
    50461 tttacctaat tctcccaact tacgctcttc ccaatcttcc gtaaaaccag gaaaccggat
    50521 ttctggcact tttgctccgt tttcagggaa cattttttgt aagaatcctt ttttcgtttc
    50581 cttcagcaaa tctaacttac gctgatgaag agcgatcgtg tcgtcaagtt gtttgaagaa
    50641 ggtgccgatt ttttgttgtt cttcctcact aggaaacatt gctactatat ttttaaactt
    50701 ttttagagac agaacatgtt gacctgttcc ctctgcttga atagataatt gtcgttggat
    50761 tccattgtta atacgtctta agaagaagtc ggaatcagca gattttaaag taaatacagg
    50821 ataaaaatag cttataatcc ctctatcaat aatgtcatta cggttaaaca caaacacatc
    50881 attatcactt cgactacgga acgtgaaata tcctctaggt agtacaaagt aaccaatatt
    50941 attttctgta gttacttgcc gatttgcaaa atagtcttct tgcaaaacta tccctttttg
    51001 ttgagatgaa gttagcatag gaaattgatt ttgtacactt gtcttttcaa cataatctac
    51061 aacgaagtcc cccaacttcc tctgttccca atcatctgta aatcctggga aacggatttc
    51121 tggttgtgta tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat
    51181 tttcaggtgt tgtttgcagg gaagagatag cttctaaaag ttttttttct agcacttgct
    51241 gttcttgtcg tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt
    51301 cttcgaaagt atctacatag cgtgggatat ttaagttata gtcattttct ttgatttcat
    51361 caaaggttgc caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga
    51421 ttttttgaat gttttcttcc gatagtttat tttgattttt ccctttaaca aattcacgac
    51481 tagcatcaat gaataataca tctcgagttt gacgattctt cttcaacaca atcaccgtcg
    51541 ttggaatcga tgtcccaaag aacaagttcg caggcatacc gatcacagca taaatactgc
    51601 catcttctag taatttttga cggatgaccc cttctgcagc gccacggaat aaaactccgt
    51661 gtggtaagac gatggccatg gtacctgttt ccttcaaatg atagaaccca tgaagaagaa
    51721 acgcaaaatc tgctttggat tttggcgcta atttcccata acgattaaac cgagaatcat
    51781 ctaagaaagt cgtatctgct gaccaattag ccgaataagg tgggttcatt actactgcat
    51841 caaatgtgta aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat
    51901 tcatttcttc ggcgtcgaca ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg
    51961 ttgtatttag ttcttgccca tgatatttaa cgttatctgg gtgtgttaag tagttacgta
    52021 cgtttaacat caatgaacct gaacccatcg ttggatcaaa aacactaaag aatcgtcgtt
    52081 ccttttgatc gagcgttacg atttgcgcca tcatatcaga aaccatatga ggtgtgtaaa
    52141 attctccggc tttcttccct gcttctgaag caaattgact gatcagaaac tcatacgcat
    52201 ccccgatcac gtccccatcg tgttctaaaa catctacatc attcaatttc ttgatgactt
    52261 cggtaatcgt cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca
    52321 catcatcaaa taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt
    52381 ttagatcgtt caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat
    52441 actctggtgc aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg
    52501 attcttcatc tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt
    52561 cctctaagga ttcatctgct aaaagaacca cttgttccaa caatcgatcg gataaatatt
    52621 t
//
LOCUS       DACDRK010000010        52450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDRK010000010 DACDRK010000000
VERSION     DACDRK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.010"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            141..611
                     /locus_tag="IWJ77_000703"
     CDS             141..611
                     /locus_tag="IWJ77_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9452348.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            1240..2343
                     /locus_tag="IWJ77_000704"
     CDS             1240..2343
                     /locus_tag="IWJ77_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9452349.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2627..3307
                     /locus_tag="IWJ77_000705"
     CDS             2627..3307
                     /locus_tag="IWJ77_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9452350.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            3397..4431
                     /locus_tag="IWJ77_000706"
     CDS             3397..4431
                     /locus_tag="IWJ77_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9452351.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            4445..4780
                     /locus_tag="IWJ77_000707"
     CDS             4445..4780
                     /locus_tag="IWJ77_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452352.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            4764..7244
                     /locus_tag="IWJ77_000708"
     CDS             4764..7244
                     /locus_tag="IWJ77_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452353.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7300..8859
                     /locus_tag="IWJ77_000709"
     CDS             7300..8859
                     /locus_tag="IWJ77_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9452354.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            8856..10220
                     /locus_tag="IWJ77_000710"
     CDS             8856..10220
                     /locus_tag="IWJ77_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9452355.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            10391..11461
                     /locus_tag="IWJ77_000711"
     CDS             10391..11461
                     /locus_tag="IWJ77_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452356.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            11476..12144
                     /locus_tag="IWJ77_000712"
     CDS             11476..12144
                     /locus_tag="IWJ77_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452357.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12164..12742
                     /locus_tag="IWJ77_000713"
     CDS             12164..12742
                     /locus_tag="IWJ77_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9452358.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13145..14056
                     /locus_tag="IWJ77_000714"
     CDS             13145..14056
                     /locus_tag="IWJ77_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9452359.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(14312..15229)
                     /locus_tag="IWJ77_000715"
     CDS             complement(14312..15229)
                     /locus_tag="IWJ77_000715"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9452360.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            15431..15868
                     /gene="zapA"
                     /locus_tag="IWJ77_000716"
     CDS             15431..15868
                     /gene="zapA"
                     /locus_tag="IWJ77_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9452361.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15926..16473
                     /locus_tag="IWJ77_000717"
                     /pseudo
     CDS             15926..16473
                     /locus_tag="IWJ77_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            16588..18948
                     /locus_tag="IWJ77_000718"
     CDS             16588..18948
                     /locus_tag="IWJ77_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9452362.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            19035..19349
                     /gene="trxA"
                     /locus_tag="IWJ77_000719"
     CDS             19035..19349
                     /gene="trxA"
                     /locus_tag="IWJ77_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9452363.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            19531..21309
                     /gene="uvrC"
                     /locus_tag="IWJ77_000720"
     CDS             19531..21309
                     /gene="uvrC"
                     /locus_tag="IWJ77_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9452364.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            21454..22353
                     /locus_tag="IWJ77_000721"
     CDS             21454..22353
                     /locus_tag="IWJ77_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452365.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            22346..23608
                     /locus_tag="IWJ77_000722"
     CDS             22346..23608
                     /locus_tag="IWJ77_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452366.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            23771..25669
                     /locus_tag="IWJ77_000723"
     CDS             23771..25669
                     /locus_tag="IWJ77_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9452367.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25968..26687)
                     /locus_tag="IWJ77_000724"
     CDS             complement(25968..26687)
                     /locus_tag="IWJ77_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9452368.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            27024..27356
                     /locus_tag="IWJ77_000725"
     CDS             27024..27356
                     /locus_tag="IWJ77_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452369.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            27343..29346
                     /locus_tag="IWJ77_000726"
     CDS             27343..29346
                     /locus_tag="IWJ77_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9452370.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            29365..29835
                     /locus_tag="IWJ77_000727"
     CDS             29365..29835
                     /locus_tag="IWJ77_000727"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9452371.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            29856..30440
                     /locus_tag="IWJ77_000728"
     CDS             29856..30440
                     /locus_tag="IWJ77_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9452372.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            30455..31441
                     /locus_tag="IWJ77_000729"
     CDS             30455..31441
                     /locus_tag="IWJ77_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9452373.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            31431..31742
                     /locus_tag="IWJ77_000730"
     CDS             31431..31742
                     /locus_tag="IWJ77_000730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9452374.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            31764..33545
                     /locus_tag="IWJ77_000731"
     CDS             31764..33545
                     /locus_tag="IWJ77_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9452375.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            33538..34914
                     /locus_tag="IWJ77_000732"
     CDS             33538..34914
                     /locus_tag="IWJ77_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9452376.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34921..35556
                     /locus_tag="IWJ77_000733"
     CDS             34921..35556
                     /locus_tag="IWJ77_000733"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9452377.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            35586..36941
                     /locus_tag="IWJ77_000734"
     CDS             35586..36941
                     /locus_tag="IWJ77_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9452378.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(37133..38527)
                     /locus_tag="IWJ77_000735"
     CDS             complement(37133..38527)
                     /locus_tag="IWJ77_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9452379.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            38619..39590
                     /locus_tag="IWJ77_000736"
     CDS             38619..39590
                     /locus_tag="IWJ77_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9452380.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            39587..40369
                     /locus_tag="IWJ77_000737"
     CDS             39587..40369
                     /locus_tag="IWJ77_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9452381.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(40538..40909)
                     /locus_tag="IWJ77_000738"
     CDS             complement(40538..40909)
                     /locus_tag="IWJ77_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9452382.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            41043..41486
                     /locus_tag="IWJ77_000739"
     CDS             41043..41486
                     /locus_tag="IWJ77_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9452383.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(41529..42338)
                     /gene="yidA"
                     /locus_tag="IWJ77_000740"
     CDS             complement(41529..42338)
                     /gene="yidA"
                     /locus_tag="IWJ77_000740"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9452384.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(42357..43724)
                     /locus_tag="IWJ77_000741"
     CDS             complement(42357..43724)
                     /locus_tag="IWJ77_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9452385.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(43708..44538)
                     /locus_tag="IWJ77_000742"
     CDS             complement(43708..44538)
                     /locus_tag="IWJ77_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9452386.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            44701..45138
                     /locus_tag="IWJ77_000743"
     CDS             44701..45138
                     /locus_tag="IWJ77_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9452387.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            45214..45834
                     /locus_tag="IWJ77_000744"
     CDS             45214..45834
                     /locus_tag="IWJ77_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9452388.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            46023..46529
                     /locus_tag="IWJ77_000745"
     CDS             46023..46529
                     /locus_tag="IWJ77_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9452389.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            46546..47445
                     /locus_tag="IWJ77_000746"
     CDS             46546..47445
                     /locus_tag="IWJ77_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9452390.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            47532..48416
                     /locus_tag="IWJ77_000747"
     CDS             47532..48416
                     /locus_tag="IWJ77_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9452391.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(48608..48679)
                     /locus_tag="IWJ77_000748"
     tRNA            complement(48608..48679)
                     /locus_tag="IWJ77_000748"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48644..48646),aa:Arg,seq:ccg)
     gene            48739..50073
                     /gene="rpoN"
                     /locus_tag="IWJ77_000749"
     CDS             48739..50073
                     /gene="rpoN"
                     /locus_tag="IWJ77_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9452392.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            50276..51313
                     /locus_tag="IWJ77_000750"
     CDS             50276..51313
                     /locus_tag="IWJ77_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9452393.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            51354..52355
                     /gene="gap"
                     /locus_tag="IWJ77_000751"
     CDS             51354..52355
                     /gene="gap"
                     /locus_tag="IWJ77_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9452394.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
BASE COUNT        17374 a         9052 c        11119 g        14905 t
ORIGIN      
        1 aagaaaagta gaagaataag ggatagatgg aatggataaa taggatttct gaggaaaaaa
       61 gcacatgata gcgttgatag gagtcaccta tgtcaggtga taaaaataca taagtagaaa
      121 attaagagga ggaagagaaa atgaaagaag ttcaattgat tagaaaaagc gaactatcag
      181 aaggcggttg caatgcatgt ggcgtagtag aagcgacaag ctatacatta aaattagatt
      241 ccaatcaagc aataatctct gaactgacag taggcggtct tgtggacagt ctggcgcttg
      301 ctgaaggttt tactggagaa gatatttatg agatgttttc agaagtccgt cagttaaaaa
      361 aaggagaaaa atgcatagag gttcatcatg agagcccgaa tgtgaggttc aaacgtggtg
      421 ataatgagat gatcttcaag aatcatgtgt ctgatcacac tgaactatat gaaatcgtga
      481 atcaaatact gtctgggatt tttgaattag gaccttatga gttcaaggaa gaaaacggta
      541 atccaaaatt aaatgaagaa tggcaagaaa cgattgaaac acaaagaaac aatccacact
      601 tatttcaata gtcttccaca gtatttcgta aaaatgttga tgaaaaagaa acaccgtgaa
      661 tcagaggtcg tgaaaaacct gccctgcatc aagtaataag aacagccaaa caaaaatagc
      721 tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagttttt gaacaccgtt
      781 taaggaagag actgtgaagt caacgttctg acctgagtat tagagagaca aatggcaaaa
      841 tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg
      901 ttttatcgag aggttgtgaa aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa
      961 aatggctttc cacatttttg cataaatttg acttaatacc gagaaaatgg cttttgaaca
     1021 ccgtttattc aaaaaaaggg actttgacac tacttttgtc aaagtcccat cggtttttta
     1081 tctattttta ttacaatcat ttcttccaga gtagtgacat ctggaatggc tgaaatgtaa
     1141 acagattatt tgcctgtatc aatgtcgaag agtaatataa aaagtgtaaa cgctatcata
     1201 ttgttcatca ttcaaatttt atagagagaa ggcattgaga tggaaaaaac atatcaagca
     1261 ggtacaaatg aaggaatagt agattttatc aatatggaag atttagaaat cgcggcttca
     1321 caagtgattc cagctggtgg ttatggttac atcagcagtg gtgctggcga cttatttact
     1381 tatcaagaaa atgaacgggc attcaatcat cgtttgatta ttcctcatgt attaagagat
     1441 gtagaattac ccgatacaac tacacatttt gacgaagaaa tgctgactgc accaatcatc
     1501 atggcaccag ttgctgctca tggattagct catgtgaagg cggagaaagc ttctgcaaaa
     1561 ggtgtagcag attttgggac gatctataca gcaagttctt acgcttcttg tactttagaa
     1621 gagataagag aagcaggcgg ggagaaagca ccgcaatggt ttcaatttta tatgagcaaa
     1681 gataatggaa tcaatttgga tattcttgaa gtggccaagc gtaatggagc aaaagcaatc
     1741 gtattgacag cggatgcaac agttggcgga aaccgtgaaa cagaccgcag aaacgggttt
     1801 accttcccgc tgccaatgcc gatcgttcag gcttatcaat caggagtagg ccaaacgatg
     1861 gatgctgtat ataaatcttc caaacaaaaa cttagtccaa aagatgtaga atttatcgct
     1921 gctcattctg atttgccagt ctacgtgaaa ggtgttcaat cagaagaaga tgtctatcga
     1981 tcattagaat ctggtgctgg tggaatttgg gtatctaacc atggtggccg tcaattagat
     2041 ggggggcctg ctgcgtttga ttcgttgcaa tatgtggcag aagcagtaga taaacgtgta
     2101 ccaatcgtgt ttgacagcgg tgtacgacgt ggacaacatg ttttcaaagc aatcgcatca
     2161 ggtgccgatc ttgttgctat tggcagaccc gttatttatg gattgtcttt aggaggaagt
     2221 acaggagtcc atcaagtgtt tgatttcttt aaaacagaat tggaaatggt catgcaatta
     2281 gctggaacac aaacagtaga agatatcaaa aaaataaaat tgcgtgagaa tcgttttatt
     2341 taagaaataa atgacgaaaa aaataggagt gcggcagatg atgccgtact cctattttta
     2401 tagttcaagg atgtcactaa tagaataatt tgaaacgaaa aaataaaatt gaaatatttt
     2461 ttcatactta ataaaaaagg aagaatattc atgtaaacga ttttattgtt tttttctctt
     2521 ttatacaaaa agggtcataa tttcaagtaa gcggatgaat tcatgaggat tttttgcata
     2581 ctgtctctac tatacttagg tcactaagaa aaaggagtga tttattatga aaaagaacat
     2641 gattgggaca ttattacttt caacacttct tctaggcggc ttggcaacac cagcttttgc
     2701 tgagggtcaa gcgacttcaa aaggagatat tacctttact gaaccaacaa ataccgttga
     2761 gcctcttaac cctactgatc cttcaaaacc agtagagcca gcagatccgg aaaatccagc
     2821 aactggccag acaggttctt taacattgga tgtagttcct gaattgccat ttggtacaca
     2881 cgagattgaa agcggaacaa aaacgtatca agtagacgct tcaaaaaacg atacacctta
     2941 tctccaagta tcagatcgtc gaggtgtagg tgcagatggc caagcacaag ggtggaacgt
     3001 gacagtttcg gtttcagatt ttgtaaacgg ttcacaagtt ttgcaaggcg ctgaattaga
     3061 ttttggaaca tccactgtta aaagtacgtc agataacgaa tccactggac caactagtca
     3121 aactgtaact gggttgagta aagcaagtgc agcgacacca atatttacag cagcaaaaga
     3181 tcaaggtctg ggaacatggc tttctgttta tgatccagca aatatcacac tgaaagtgcc
     3241 gaaagcagca gctggaacat tcacggctga tttgacttgg aacctcgtgg ctggcccagt
     3301 agcatagagt atgaaataaa ttcaacaaac tgcggtgaaa atactatagg tgaaggattt
     3361 tttaccgcag ttgttgttga aaggatgtgg tggacaatga atcaaagtaa acgactgttt
     3421 gtttcgtttt tttctatttt aagtctattt ttcatttttc cgtcaatcag ccaagcaaag
     3481 gattcagcag gagactttgg aatcaaaccg gtatttccag agaaccaaat agataaagca
     3541 atcggttatt ttgatttatt agtggcaccg gaacaaaatc agacactaga agtaattatc
     3601 agcaattctt cagatgaaga acgaacattt gaggtctctg tcaatccggc ggtaacgagt
     3661 gatggtggaa cgatcgatta tagtcaaaaa aacccaacgt tagatgagac attgccgttt
     3721 gatgtccgtg acgttttgct cattgcgaaa aaggaaatca atgtatcagc acatgcagaa
     3781 acaactatac ctatagaagt gaaaatccca gcaaaaagct ttaagggacg tgtattagcg
     3841 ggtattcatg tttcacctaa agaagaagct gaaacagaaa atgctacaga aggagcacaa
     3901 atcaaaaatc gtattgctta taatctcgca gtcgttttac aagaaagtca agagacgata
     3961 gaaccggatc tgaaactttt atcaggagat ttggacgagg tcaacgcaaa gccaactgtt
     4021 cagcttcgtt tccaaaatcc tcagcctagg attatcagca atttgatatt tacgagcaag
     4081 atcttttatg aaaatcagct ttatattgaa aatacgtcta atgaattttt agttgcacca
     4141 aattcgaatt ttcacttgaa tcttgattta gctggtgata aagcaaaagc cggcgattac
     4201 cgcgcagaaa tcatagcaaa atcaggcgac agtaatgaat ggcgatttac acaaaacttt
     4261 acgatcaaaa aagaaaaagc acaaaaagta aatgaaaatt ccgtttttgc agtacaagaa
     4321 caatctttcc catggcttta tgcaggattg gttgctgcag ttctagtgtt atggttgatt
     4381 ttgctgtttt tgtgggtcca caagaaaaag aaaaaaggag aaacaacatg atgaggtgat
     4441 ttcgttgaaa acatcgatct tattgggagg gattattgca agtttgctgt tcttcagctg
     4501 gcaagagcca gtccaagcag ataccaaaag ttcaggtacg attacatttt atgatctaag
     4561 tccagttccg catccaagtg gttcagtcaa tggcgcatca aaacagcaga cacagctacc
     4621 gatttataaa gaatctaatc aacatggcgt attcccggag acgaatgaag aaaagatccg
     4681 ttttctttat ttgatcattg gtatagaatt atggcttctt gcgtggttgt tgttatggag
     4741 aagaaaggaa aaggaggatg aacatgttgc tacaaaataa attttcattt tttcgctcct
     4801 tcttttttac ttcgcttttt gttttaagta tatttatctt ttcaccttcg gcagaagctg
     4861 ttggagcaac gcctcctgcc atttcttatg atccatacac gtatgaaaat atcgtacctc
     4921 caaatggtgg acgtagtgca gtcaatggcg ggaagcttgt ccaactaact ggggctaacc
     4981 aattgcctaa attgactgct acttatttga acgatggttc aaatgatcct tggaaaggtg
     5041 gcacggtccg ttttcattgg gaaagccatg tgaaactaac aaatgtactt gatttggtga
     5101 atatcactgc acaaggattt gcatttgtgg cgatcatcaa tttgccggaa catgtatcaa
     5161 gcagtgatgt attaaaagca attgactgga acaacgccta cttgtatatt gccggtgccc
     5221 ccatcaaggt tactagcggt agtttattat cagcaggaag ccataccttg cgttttggga
     5281 tggggtcgtg ggatacaaat actctattgg cagatattat tgctattatt acgcgaggcg
     5341 aattcaacgt ggacgatatc ccgcttcaat ttgatgttac agctaatgtc gcgaagatga
     5401 cagaaaacgg agataaatat gatacgagtc caaacaaaat tttgaccaag ggtaaattac
     5461 aaccttctat gtctaaaaag gccgtttttt ctgttgattt ttatgattcg aataatatta
     5521 tccaagatcg tatgttcaat tcgattttag ctccagctgc aggtagattt tatcctaaat
     5581 taactggcgg aaacttgaat ccaggagcat atgcattttc tgcaagtgca gagataaata
     5641 cgtggaatag ttacatctca acaagtgatt caacaggaaa atataataca tttgctgata
     5701 ccacagaata cgtggatgga aataaccaga accttccagg ctctaccaat agtcggacat
     5761 tgacgatggg gctgactgaa ggacgttttg atcagttgcc atttagtcgc tttgatcgcg
     5821 tagtcaacta ttttacagga aaaaacgtta cacaaggagc aatactgact catactccag
     5881 caagcacaag tggattgaat cagacagcca atatcgtgta cagtgggaaa gatgcgcaag
     5941 gcggattgct ttctcctgtt gctttaaagg tattggagca gtacaagcta gacggaacgg
     6001 ttttaccaac aaacttaaca gccgatagtc agtggggaaa gctcctttct tctgcatcgt
     6061 accaagtcac ggccaattgg tcagcaactg aactaaaaaa gggttcgatc cattacaggc
     6121 tttattcgaa aggaacccaa aaattagcga caggatggac agatcaactt ttccaaacga
     6181 tccaatcaaa taacgggggt gtaaatacag ctacttctac actacctgca ttggcttcag
     6241 ggcaatatta ttttgattat cgcttggtgg acgatgtact tactaacatg tatccgtctt
     6301 ttgcgtacaa atggcagtct gaacaagctg gaatcaccac tcttcctcaa ataacagtgg
     6361 ctaattttcc atctgtgagt gcagatagtc agctaaaaaa ccttagcaga caacctgaga
     6421 caggagatcc cctgtctgct ctatcaggag atacgattca agaaaatgtg acattcacgt
     6481 taacaaaatc tggggatgcg ataaccgaca aaaaaatcac gatctcactt cctgaaaata
     6541 ctacttatat caatggttcc ttaaaattaa atggtacaac tatcgcagat accggcattc
     6601 aacaaggagt ttctgtccca gcagatctat tgagcaaaat aggagatacc attcatctta
     6661 cttacaacta tcaattaaat actgtagata cttctgtaca atccgtcagc atactgacaa
     6721 aggcagctgt actttccagc aatattacat tagcagatgg tgctaagctt cctaacccag
     6781 ttgtccaaac atctgctaaa acaattcttg tccccaaaca agaattaaca ttagttaatg
     6841 taccagatga ttttacattt ggtaacgatc tccccaaacc gttgaagaca agttattatg
     6901 aagcaaaagg cgatttttct tttgatgtgc gggatactcg gcttccaagc acgtctcctt
     6961 ggcaactgac agggacattg acttctttat ttaagaatga tcaaggtcaa gaattaagcg
     7021 gaacaaaatt atacttcaat cattctggat caaaacaatt gattcagcaa ggtcaaaaca
     7081 cgttgattta tgagagtgat ggaacagcta aaggagaggt tctcgttgac tttcccgata
     7141 ccgatggttt actcttagag gtaaatagca gtacgaatgc tcaacctggc gcaacgtatc
     7201 aaggcatggt cacatgggaa ctgacagctg ggccaaccag ctaagtgatt agaatttgta
     7261 tggatctgtg ataactgtca cagatcctct ttttcaagga tgagaaggaa acttctgctg
     7321 ttaaaatcag aaatgcttgg aggagtggac ttggaacttt tcagtttgct tgaaaaaaat
     7381 gaacaacggc aaatcaaact tttccaactt ttattagagg aaaaaggaga gatttccgtt
     7441 aataacgtac ttacccgctt atcagttgat cgggctacct tgaaagaaga tctgttttat
     7501 ttacaagcta acttggaaaa tatcaaacaa ttggaaattg ttatccaaga agggaatatt
     7561 ttattaatca gacatggaaa tatagcagca gtcgatgttt attatcattg ctacttgcgc
     7621 tattctacaa aatatcagat acttctttat ctgttggaaa atggctcatt tgatcggcag
     7681 aaacttgtca tggaacttaa tatcagttcg gctactttgt ctcgcagagt gaaggaatta
     7741 aatgattctt taaatgagtt tcatctgaaa ataaaaagcg ggcggctgac cggtccagaa
     7801 agtcaaattt gttattttta tttccaatta ttttggtttg gacgtcctta tcttgaaaac
     7861 aaaaaagaat acagtgattt atcaggaaac acttttttag atctgctgca aaaagagttt
     7921 agttttacta ttacagaaga aggacgattg aaatttgaaa tatggctttc cattatgaag
     7981 aagcgaatgc gctgtacgaa aaaatccaaa ccagattata gcaatcttcc tgttggattg
     8041 tttcaaacga cacaattttt attggacctc catacatttt tgacccgtta cttctttcaa
     8101 tatgcctttc cttggaatca aaatgaaacg ttggtttttt atcttttcat gatcagtaac
     8161 tttacattag atacacataa ctcgcatgtc caggagtttt tgataagcga aaaatacaaa
     8221 gacgacgtag tagctcaatt gaatcgaacg tttaaacaat cgatagaaga atatttctct
     8281 ttttatgttt ttccagaaga gatttcgcaa aagattgcgt tgacagtgat gcaaaatcac
     8341 gctcattttg cctattttag aggatggatg aatatttttg gccaagaagg attaaaacag
     8401 cggttaactg aagtagaaga taaacaattc ctggttttat gtgaaagatt ggtcagacaa
     8461 acatttgcta ttgttgattt tactgaagta gacgaggaaa atgcccgctt tgaattattt
     8521 gctcgctatg catctatttt gcggacgctg atcaagcagt tagaattacg tctgcaaatt
     8581 gcttatgatc ttccttatga aaggctaatg gcggatatcg tgaaagaaat catccaggaa
     8641 caattgctga atacgatcaa atatgattta gtcgaatacg agaaagaaaa gcagtatgat
     8701 atcattttga ctagccaatt gaaagagtac ccgtcacaga agtccgctaa agtttttgta
     8761 ttcactagca acgaatataa ttatgatttc ccctatttga accaattttt aaaagaatgt
     8821 tatttagaaa aattgaattt aaggatgaat aaaacatgaa aatcgtgatc attggtgctt
     8881 cccatgcagg catcacagct gccctaaatc taagaaaact tcaaccgaaa gcagaagttt
     8941 tactgatcga tgaagatcat aaagatggac taggctatgt ttcaaatgga atcaaccttt
     9001 atttaaaagg aaagatacgt agtttagctg aagttgccca taacatgcgg actcttcaag
     9061 aaagtggggc aaaactgatc accgaatgga gagtaactga acttgatcca gacaaacatc
     9121 agctcatcct cgcatccaaa gaaggaaaaa acgagaccat aacctatgat aaattaatcg
     9181 tagcaacagg ttcctctcct gtcaccttgt ataaacaaat tgaagctgaa aatgtctaca
     9241 cctataaaaa tctagtgcaa tctaagcaag tacttgccgc tttaaaagaa gcaaaagaag
     9301 tagtgatctt cggtgctggt tatattggat tggaattagc agatgctttg agaaataaag
     9361 gatacattat tcatctagtt gattatatgc cgaatgtttt atcaagatat ttcgataagg
     9421 atatgatcaa cagttttcaa aatcagctgc aaactaagca aatcaatttt tatccaaatg
     9481 aattcttgat cgattggaaa aaatcagaag agaaagtagt atccgtacaa cttctatctc
     9541 aagcaataaa ggcagatatg gtcatttttt ctgcccaaac tcgtcctaat acgacattgt
     9601 taaaagaaaa agtggctttg tatgaggatg agactgtgat agtcaatgaa tatctgcaga
     9661 caagtgatcc ggacatctat gcgattggag atatcgtccc agtcagtttt gacaaaaata
     9721 aacggcatct ctttttacct ttagttactc gagctgtaca tatggcaaga gcagtagcgc
     9781 taactttgag tggtcagcca accgcttacg acctcagaca aaagataaca gcagcagtca
     9841 ttatagacta ctttttagga actgtagggc tgacagaaga tgaagcgcca ttcttggaac
     9901 aaaataccgg ttcgtgttca ggagaatttg atctatttcc acaatacgat gaagaaaaca
     9961 aaacagtaaa tgcaaagctg atctaccatc cggatacatt agaaataatt ggcggacagc
    10021 tgataagcca agaattctta ttatcagatt tgaacctgct tgcggatatc gtgaaacaca
    10081 aaactacgat tccacagcta gcagttgagg attttggatt tctgtctgaa cacacctctc
    10141 gttttcacta cctcaatgag ttagctttta aagtactagc taagagagca aatagcaata
    10201 gagtagataa aagactgtga gaaatatcac ggtcttttat tgtgtgggga tataataaat
    10261 attaatcaat cgagaataaa aaattcatat aatgaaatag cggaagaatt ttttgagcat
    10321 aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt atttcaggag
    10381 gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac gttatgcgtt
    10441 agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg ggttagctta
    10501 tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt cgattgtgct
    10561 ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga tagcagaaga
    10621 agtcgaaggt gggaaagtgg cgtatcttgc ccagacacct ggagtcgtga ctagtaaaga
    10681 cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa atcaattcat
    10741 catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag taggcgatat
    10801 tagtttgaaa gaagaatatg gtctagccgt aggagataca gttaagttat ctggtagtga
    10861 caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct caccagttct
    10921 ctatacaacc atcaatgctt accaagagat ccgcttcgaa aaagaagata caagtgaaaa
    10981 tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg aagatttaga
    11041 acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc aagtattgac
    11101 ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg ggattttcat
    11161 ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac aagggatcac
    11221 aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat ttgttggtat
    11281 tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg cggttccatt
    11341 ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgctggtag tagctgtgtt
    11401 aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg caattggata
    11461 agaaggagga agagcatgaa agcaatagaa tttaaagaag tagaaaaaag tttccaagat
    11521 ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa aggggaattt
    11581 gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt ggcaggcgga
    11641 ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag taatcaaaaa
    11701 gaaagtgaac gcgttaaact acgttttaaa gaaattggct ttattttgca agcttccaat
    11761 ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat caaacatgaa
    11821 catcgtcaaa aagaagcaaa agaattattt gagcagcttg gcgtggataa gttgatcaac
    11881 aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc tcgtgcattg
    11941 tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga ttcagaaaag
    12001 gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa agcaacgatc
    12061 atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat gatggaagat
    12121 ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc atcgatacaa
    12181 caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt ccaagggata
    12241 tctcggcact tcaacaagag agatcgcaca aaaagttggg atcacccaac ccaatctcta
    12301 tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc acttaaaaga
    12361 tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc aagtaacctt
    12421 gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc tgatgcttca
    12481 cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc tatggaagaa
    12541 aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc taagaaatgg
    12601 gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt acatcactcg
    12661 gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt tatatttgaa
    12721 tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaaatagtta ggtgaagaag
    12781 caagatatga cgaagatcgt tatatcttgt tttttttata taggtagttt ttcataaata
    12841 caaatttgtt attctctcat gattatcagg gtatattttt atttattatg tcggtgttcc
    12901 aggaagtgtt ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa ggaaactatt
    12961 tattgggtag atgaatcacc acgaatagtc aaaaggagat ttcaggatat gccaaaaatg
    13021 aaaaaaatca ttttagcagt aatgttattt tctacggtta tatcttttac agcccctttt
    13081 ttgcgtactt aagctgattt taagttttga cccttagtct ggatctacga ggtgagagtc
    13141 aagaatgaaa aaaataattg gagcttttgt atttgcaatc attatcatta ttatctgttt
    13201 tcaaatagat gtttttggtc aaaaaaagat agttggacaa acggtcagca gtacgaacag
    13261 tacggttaca aagacaaagg aatcatttga tgatacagaa caagagacat taatcaaaat
    13321 cggaaaaaat gaccgtacac tatatacaga tgactcgcta actaccccac ttgcaactat
    13381 aaacggcgga gagctggcgg agtttctttc tgaaacaaaa aatgcttatc agatcaagac
    13441 aaatgatggc tatactggat atttagccct cgaagatgga accaaagtca caaagaatat
    13501 acaaacaaaa ccggaagagc tatctgatgc agtgattgta ttagatcctg ggcatggcgg
    13561 aaatgatact ggggcattaa gcaatgatga acaaacagaa gaaaaagata taacattaag
    13621 tacagccata aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt atttgaccca
    13681 tactacagat gaattagtac agcttggaga tatatgcgac tacagtgaag aaaaaaaggc
    13741 agatgtcttt atcagtttac acgcagactc tacggaatat gcgaatgaag caactggaat
    13801 cacgacatat tattactatg gacaagagga aacattagca cagacaattg ctgactcctt
    13861 cactgatctg cctctggatt caagaggaat ctccactggg aactatcaag ttcttcgaga
    13921 aaatcttcaa ccttctattt taatcgagat gggttatatg aataatgata gtgatttaaa
    13981 ggaattagta acagacagtt atcagcagca gattgctgca agtttgacac aagcattgac
    14041 cacttatttt cagtaataaa taagcccagc caaacaaaaa tagctcctca tatttttcgt
    14101 ttggccgggc ttattatcgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg
    14161 agcctgccgt ttaactccgc gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg
    14221 atgactattg tgacttgtga ccgaggagtt ggcatgagaa caccgtttat tcggaacaag
    14281 ggactgtgac atgacttttg tcacagttcc attatttttt tagcagcttc aaagcttttt
    14341 ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg cctttctcca
    14401 atagttttgc cgctacttgg tcagatttcg ttccagctcc tgatggaagg gtgattccag
    14461 cttcagctga tgctttgtgt aactcttcca aaaagctggc acgactgatg atagaagcag
    14521 cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt tcttgtaatt
    14581 gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac tgatcgatca
    14641 aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc gcttgattgt
    14701 gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg tatttttctg
    14761 gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca agctggatga
    14821 tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat tctgcaagct
    14881 gatcacgttt cacataagca gcacatactg ttagcggacc aaaatagctg ccatttccga
    14941 cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa gaggctgttt
    15001 ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct attgctgcat
    15061 tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc ccgtcttttt
    15121 ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga tagtatgctt
    15181 tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata aaattctcct
    15241 cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa gagattgcga
    15301 gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa atatttgtgg
    15361 agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca aataggagga
    15421 aatgattcgt atggcgcacg aaaaaacacg ctacaaagca gtgattgcca accaaacata
    15481 tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc tgatcaatga
    15541 acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag cagcaatctt
    15601 gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatct tggagctgga
    15661 agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg aaaatcgtgt
    15721 gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa atggtcaagc
    15781 agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga aacgaaaagg
    15841 acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa aaatttaaaa
    15901 aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt tattgctaat
    15961 tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg cgattggata
    16021 tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc atttggaatt
    16081 atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg atcaggtatt
    16141 tgcttttcac ctagatgaag ctttttatgc aggaactgct tttctgatta ttttgctaat
    16201 tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt atgtgccgat
    16261 cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg cttacgttac
    16321 tatatttttg attttgcagt tacttgcctt tgttccatta gtgtggtcca aaatcaattc
    16381 ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac caacaagatg
    16441 tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa aatatttcgt
    16501 tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc acagctcttt
    16561 tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac attggaattt
    16621 gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa agaagaactg
    16681 gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca agaaactgag
    16741 gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaact tgaaaatatc
    16801 cgtccccata tgaaacggat cgagatcgga gcagacttga atggagtgga gcttgcacaa
    16861 gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga tctttctgac
    16921 agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac gatccctgtt
    16981 ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga tgacgcttct
    17041 cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt tcgtgaacag
    17101 ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat catcaccatg
    17161 cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt tggcggtgta
    17221 gtccacgatc aaagtgcttc aggacagact ttatttattg aaccaaaaca agtggtggat
    17281 ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca acgtatttta
    17341 agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa tgcttatgtg
    17401 attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt aaaagccatc
    17461 gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca tccactgatc
    17521 gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca agccatcgtc
    17581 attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg tctgttacaa
    17641 ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat cggtattttt
    17701 gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct atcgaccttt
    17761 tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa aagtctggta
    17821 ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct agcaatcgca
    17881 atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca ttatcctgaa
    17941 ttgaaagttt atggatacaa tcgggcaaat acgattaatg ccagcatgga gttcgatgtg
    18001 gatacactaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag caatgcgttt
    18061 gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa gcagttgatg
    18121 aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg ccggaaaatg
    18181 gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga acttcacgat
    18241 gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat ggaaaaagca
    18301 aagaaaaaag caaacgaagt cgtgtcagag gcggaagaaa aagcagaaaa aatcattgct
    18361 gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca tcagttgatc
    18421 gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa aaataaagta
    18481 ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt ggttacgact
    18541 tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt agaaatcggt
    18601 atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca aaaagagccg
    18661 acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc gaatcaattg
    18721 gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata tttggattct
    18781 gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac aggcgcactt
    18841 cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt tgagtttgca
    18901 ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag cttactaaag
    18961 gtaagcagat ggttcgctat ccaagtatag aaatgctata ctaatcatag attttaaaaa
    19021 tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa gaaacagata
    19081 aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt atgcaagcac
    19141 cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt gctaaaatgg
    19201 atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt ccaacattaa
    19261 tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca aaagaacaac
    19321 tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc tgagggtggg
    19381 actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt caaattaata
    19441 ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca tgctataata
    19501 actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa caaattagct
    19561 ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac gatcatttat
    19621 gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg cagccataat
    19681 acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt tacagaatcg
    19741 aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc taaatacaac
    19801 attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga aaaatatccc
    19861 cgtttggtga ttactcgtaa agtactaaaa gacaaagcac tgtattttgg accatatccc
    19921 gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc tctgagaaaa
    19981 tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca atgtctgtgt
    20041 ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga agtgaaaaag
    20101 tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat ggaaaaagct
    20161 gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg tgccattgag
    20221 acaatcatga ctcgccaaaa aatgacaaat actgatttac tggatcgaga tgtatttggt
    20281 tatgcgattg acaaaggatg gatgtgtgtc caagtgtttt tcgtaagaca aggaaagcta
    20341 attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga tttcttgact
    20401 tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt cttgattcct
    20461 gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct gcaaccaaaa
    20521 cgaggagaaa agaaaaaatt agttgaattg gcgaataaga atgcctcagt tgctttgaat
    20581 gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt tgaacgttta
    20641 ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa ctctaatatc
    20701 atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc ggcaagaaaa
    20761 gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc atcaatgaga
    20821 gaagtcatct atcgcagata ttcacgagtg attcgcgaag ggttgccttt acctgatttg
    20881 atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt agaaaatcaa
    20941 ttaggaatcg atattcctat tgctggcatg gcaaaaaacg ataaacataa aacaaatgag
    21001 ttgctattcg gtatagattt ggctgtagta ccgcttgagc gaaattcacc agagtttttc
    21061 ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca tcggcagcta
    21121 cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aagggttagg accaaaacga
    21181 aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc cacgacagaa
    21241 gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga taaattacat
    21301 caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt ttatgtacaa
    21361 caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg gtgtctaagg
    21421 atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga tcttgttaaa
    21481 acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga tggaaaaatc
    21541 atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt gatcttggat
    21601 tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg ggagaaagat
    21661 tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt aacgattcaa
    21721 gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat tgaaccaaaa
    21781 atcgattttt ggatggaaaa atttcaggta aaagggaaaa agacggataa agtaaaatct
    21841 ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca tgaacctaaa
    21901 ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga attgctaaaa
    21961 gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag ccacaatatg
    22021 gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa aatggttttg
    22081 aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt cttggaatct
    22141 ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat ccttcatgaa
    22201 gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat ctttactcgt
    22261 gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt ggaagagatt
    22321 ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca aagatgtcta
    22381 tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt ttatcatgct
    22441 gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca ataaaatcgg
    22501 cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa cagatgattt
    22561 tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg aagaaaaaat
    22621 cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc tttatagcga
    22681 aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg gcttgcaatc
    22741 gatgcttcga gcaagtcaat taggcatttc accagaagaa gtagctagct tgagccaaca
    22801 agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga cagttggaga
    22861 agataactcg acagtccaat atgtcgtaag ttatgtagca acgattgtct tgttcattat
    22921 tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag gcacgcggat
    22981 catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa aactaacagg
    23041 tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg gtataggata
    23101 cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct cattggataa
    23161 cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca ttttgatttt
    23221 ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata cagcaaaagc
    23281 aattttgcca gtgacttatc tatctctagg cggatatatg cttggattga tactaggagc
    23341 ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct tatcttcata
    23401 tattatgccg gttcgcttag caaatgagac agtcggtatg ggtggagcat tgatttcctt
    23461 gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca tgtacaaatc
    23521 gaatgtactc gtgtatagcg aaggcggatt atggagttca ttgaaacaat cgatttcaat
    23581 tatgagaaat gaacgaaaaa aagcataaaa tatagaaaaa tatagaagga agctggacat
    23641 ttattgttcc cagcttcttt tttgtataga cctatttcta tttttttatt atattggata
    23701 tatatattca ttgaaaatag agcataacaa ctttataatg ttgagtatac caatttttgg
    23761 aggcgtttta atggaccgct atttagattt actttcagag aagttcccta caacggaaga
    23821 tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag gaacagagtt
    23881 atttttgagc gatatacatg gcgaatttcc tgcttttgat catattctgc gtatcggttc
    23941 aggaaatctt aaagaaaaga tcaaagacct tttctctgaa caaatgacgg aaacggagat
    24001 cagccaattc actattttta cggcttatcc agaatatgtg ttgacgactg attggtacaa
    24061 aaaacaagat aaatcccagc tgatccatcg tttgatcgat cttttacggt ttacgacagt
    24121 caaatacact cgatcaaaag ttcgaaaagg attgccgaaa gaatatagct atatcattga
    24181 ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa gactatgtaa aaaaaatcat
    24241 tgaacaactt gtcttgatga aggaagaaga aaaattttta acaaaacttg cgcagacgat
    24301 ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat atttttgatc gaggaactca
    24361 agcagccaaa gtaatggatc gaatcatgga tttttcctct gtagatatcc agtgggggaa
    24421 tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttat tgacgctttt
    24481 acggattgcc gcacgctaca actatttatt tgaattagag aaagaatatg gattaaattt
    24541 acgtccatta ttttcttttg cccatcaaac ctatcagaag aatccagtat ttacccctaa
    24601 aaacaggaaa gatctatctg aaagagagca agaagaatta gaaaagatcc atcaagcact
    24661 ttctatcctc caattcaaat cagaacatca gttgttgaat cgacgacctg aatttaaaat
    24721 ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa tcaacgatcg ttttagaagg
    24781 tcagctctat gagttgaaag ggacttgttt tcaaacgatc cagccagatg atcctaaaaa
    24841 attcttggaa gaggaacaaa aagtgatcga tagtttgatg tattcattcc agcactcgtt
    24901 aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg ggaatgtatc tcacgaacaa
    24961 tcagcatgta ctgttccatg gctgtatacc tgtagatgaa aaggggcagc tcttagcatt
    25021 tgaattagac caatcttatc gcggaaaaga actgctggga ttttttgaaa aacagataac
    25081 tgaaagcgca aaagatttga ctgcaaatga agatttggcg accgatttga tttggtatgc
    25141 gtggagcggg ccattttcgc ctttattcgg caagagtaaa atggcaactt ttgagcgcta
    25201 ttttctcaca gagcctcata cgcacgtcga aaaaagcaat ccctattatc acttgcgaga
    25261 tgaagagtgg ttcagtgaaa aaattttggc agaattcgat gcaaaagaag aaggttcagc
    25321 aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga gagtcaccga tcaaagcaaa
    25381 cggaaaagca tttgttattg acggaggtct atccaaagct tatcaaaaaa caaccgggat
    25441 tgctggctac tcattactat gcaattctta cggtttccaa atcgtgacac attatccgtt
    25501 cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa acgaatgtaa aaaaagtgat
    25561 cgagcagcag ctcccacgca aacttaatcg gaatacgaca aaaggaatgg aattacagca
    25621 acagattact caactccaaa ggctgttgga ttatcgaaag gacgattgag aaaaatgcta
    25681 gccaacattg ctcaatagac taaaaagcga ctgcaacaaa aggcgtgtcg cagtctcctt
    25741 cggaattagc tcaaaaaacg gtaaaatatt agaagctatt tttctgttta tttctctaat
    25801 actcaggtca gggcgctgat ttcacgacct cttccttaaa cggtgttctc ctgccaactc
    25861 ctcggtcaca agtcacaata tccaacaaac atgagaagct gttttttgaa attgctcctt
    25921 gtgactcaga gttgaacggc aggctcacga cctaatcaga aaaacgatta aaattcattc
    25981 acaaagaaat cgaataaacg ctgttcagat gaatctacac gatgagaaag ttccggatgc
    26041 cattggacac caaggatttt gtgcatatta tctttacttt ggactgcttc aatgagtcca
    26101 tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat ctttgactgc ctgatgatga
    26161 taggaattca caaagtatga atcagggaga aggtcactta ataaactgtc cgaaacgatt
    26221 ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt gttcgtgttt tacggaccat
    26281 tttggatata atgaaagatc ttgataaagt gttccctcca acacgacatt caatagttga
    26341 agtcctcggc agactgcaaa aatcggtttt ctttgtttca atgcttccaa gatcaatgcc
    26401 tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt ttggatgagg gtcttccatg
    26461 taaagttgcg gactaacatc ctgaccgcct gcaagaatga gtttgtcgat tttctcaatt
    26521 agttgaggcg ctgaatctgg agacatgact ggcagaataa gaggtaatcc ttgagcatct
    26581 aaaactgcat ccacaaatcc ctgcggtgta tagcttactt gatttccttg gaaagtatct
    26641 gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg gtttcatttt tattccccct
    26701 aaagtagact aattgtaaca aggatttaaa aaaatttaaa ctatgtaaga cttgccccta
    26761 tacttatctg ttcttgctgt gttactatct gtaaagagaa ttttcgtgtg aaattcatga
    26821 cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa taaaaaggtt
    26881 gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt attgaagggg
    26941 cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag aaagcatgta
    27001 cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa caaatccgaa
    27061 aagcagaaga agaaaatgag aagcgtgctc gaaccctacg gcagcagttg aagacctatg
    27121 cagaagaaaa agagcaagaa ttggagataa tccaaacgaa cagtcggcaa cagctgaaac
    27181 agttggtaga agaaaaagaa caattcgaaa agcaagaatt agaaaaagaa aaaatgaaat
    27241 tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga ccaatatcaa aagcatcaga
    27301 gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac ttatggccgt cactaagatg
    27361 gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca agcagtacaa
    27421 ggaatccaaa atgttgaaat ccgggatctg tatcaaaata gaacaaacaa ccagtgggta
    27481 gaaaaatatt ttccagatac cacgattatt gataaagaag caaaattaag cgctttaggc
    27541 aatcggctga aagacatcca agaagctatc caattcatac aacaccacgg cgatagccgt
    27601 caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagacttgga agcaaattat
    27661 tccgaagaaa atttcatgaa taaattagaa gaagtccttg atttaaaaga aaagtgggaa
    27721 cagctaactg aaaaacgtga tcgtttagaa gaagaggaag aatggcttct tcattggcaa
    27781 cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga gatggggact
    27841 gtgagtgcag caaattttga aagtttcaag gaagaactaa gtaagcttga tgacgtttat
    27901 atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc tttaaaaaaa
    27961 tcagttgctg ctatagaaga aattgctggg aaatacgggt tcaataaaga aacgtatcct
    28021 tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac agcaattatc cattgttcac
    28081 gagcagcaaa aaaagataac gacagcgtta ggtcagtgca gcggctatat ccaagaattt
    28141 gaatgggtag aagaagtcac acttgccgtt gcagaacggg aaaaagtaaa agaacggttt
    28201 atccatgcca cttatctgat cgttttgcaa ggttgggtag atgcagaaga aaaacagaat
    28261 ttgatgcatg tgttaaacga aactgtatca gaagaagata tatacgttag tttcgaaaca
    28321 ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca tccgcttatc
    28381 gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga agtagatcca
    28441 acgccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc agatgttggt
    28501 tacggattgc tgatgctcat tgggacaata ctagctcaaa aactatttgt tttaccccgc
    28561 gggatgaaac gtttcgtaaa attctttgag attctctcgg taccatcgat catctgggga
    28621 ttgatctaca gttcattttt cggacaggca ttaccaaaag aactatttgg tatccgcttg
    28681 cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt atccgtgatt
    28741 ttcggtctga tacagatctt ggttggatta ttcgtggcag caaaggaaaa tatcaggagg
    28801 aaagattact tgagtgctgt cagtgatggt tttgcctggc aagggatcct gattgggatc
    28861 gttattgcat tgattggtgt catgttgata aaaaatacag catttgttta tcttggcgcg
    28921 gggctatcga tcattgctgc tttatgtatc ttaatcgtgc cgattatcca atcgccgtct
    28981 aaagtcaagg gtgctgccaa aggagcgtat aatctttacg gattgactgg ttatatcggt
    29041 gatttagtca gttatacacg tttgatggca ctaggtattt caggcggaag tattggcgcg
    29101 gcattcaata tgctagttgc tttcatgccg ccagccgctc gattcagtgt agggatcctt
    29161 ttgatcatcg ctttacacgc actgaatatg tttttgacac tgttaagtgc ctatgtccat
    29221 ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt atacaggagg cggacgtgcc
    29281 tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca atcataagaa aaaaaacaat
    29341 aaataattgg aggattttta tacaatgatg gattacttaa ttagtcaaaa tggtggaatg
    29401 gtatttgcag tattagcgat ggcaacagcg acgatttttt cagggattgg ttcagctaaa
    29461 ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa caagtcaacc cgaaaaattc
    29521 ggacaggctt tgattctgca attgcttcca ggtacgcaag ggttgtatgg attcgttatc
    29581 gcctttttga tttttatcaa cttaggaaat gatatgtcag ttgtacaagg attgaacttt
    29641 ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt caggtatcgc ccaagggaaa
    29701 gttgcagcag caggtatcca aattttagcg aaaaaacctg aacatgctac aaaaggaatc
    29761 atttttgctg cgatggttga aacatatgcg attttaggtt tcgttatttc cttcctatta
    29821 gtattgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa aaaatcatct
    29881 ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt agagcaagaa gagcgaatga
    29941 agatcgatca aaactttgaa caaaaacgta cgcaaatcga aacagaacat caaaaacaaa
    30001 aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca gcttaggaat cgccaacaag
    30061 tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttacgacga ttgtttgcag
    30121 atgcaatagc tgaaatggag aattgggatg aatctgaaca aatccagttt atcaaaaatt
    30181 cattgtattc attacctttg acaggcaaag taacattcat tgctggagaa aaatcagcag
    30241 cttatctttc tcagacattg ctggatgaat ggaacaatga acttcccttt atgatggttt
    30301 taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa ggagttcagt
    30361 ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt tttgagattg
    30421 ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac taaatccctt
    30481 gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga tgattcaaac
    30541 aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc catatattta
    30601 tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc gtctatttcg
    30661 ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga tgcgctatac
    30721 tttccataat ctgaaggttt tgacaaaagc cgagatgaca aatcaaaatc tggaccattt
    30781 gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc atacgcaagt
    30841 ttcaaatgaa ctccctgaaa gcattatgga cagtatacga gaagtacgtg attattttga
    30901 agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt tgacagagca
    30961 gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca ttgcttttat
    31021 tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa catatgggtt
    31081 catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt tgaactttgt
    31141 agataaagat atggaagatt acacgaattt tctattagag acagattatg ctgattttct
    31201 gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag cttgaacagt tgaaagatga
    31261 ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac tgccactact
    31321 agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg ttatcggcaa
    31381 aagaagccat ttcgaaccag aaacaatcag aaagcgggtg agacaagtct atgacgcata
    31441 agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt ggctttgatg
    31501 ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg gcaaagaatg
    31561 agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa acgatcgaac
    31621 gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa ggatcattag
    31681 ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa aatatcttat
    31741 agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc tccggacctc
    31801 ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta gtgggagatc
    31861 taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt caagtatatg
    31921 aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa gctttatctg
    31981 ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg ccattagata
    32041 cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg cccgcattgg
    32101 accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg gtgagtgctg
    32161 gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc atggtgccaa
    32221 atgggataaa aggaacgatc aaaacaatcg aaaaaggttc attcactatt gatgaaacag
    32281 tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa aaatggccag
    32341 taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg atcactggac
    32401 agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct gtacctggtc
    32461 cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc gatgtggaca
    32521 tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt ttaaatgaat
    32581 tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg gtattgattg
    32641 ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg ggaatcacaa
    32701 ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac tccacttcac
    32761 gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct ggtgatgaag
    32821 gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct ggaagagtca
    32881 ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct gtatccccgt
    32941 ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta aaagtattct
    33001 ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat tggatacaaa
    33061 gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt caagctgact
    33121 gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag ctgaatgaaa
    33181 tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca ttagaagtgg
    33241 caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc gatacattca
    33301 cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt gaagaaggac
    33361 gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc gtatcgattc
    33421 gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa ctagatcaaa
    33481 ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc atgagcaatg
    33541 attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt tgaaaaagtt
    33601 gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga gattcgtcgt
    33661 ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga aggaacaagt
    33721 ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga actaggtgta
    33781 tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga taacggacca
    33841 gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc agtagctcgg
    33901 gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt gaatacatta
    33961 gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa agaattagct
    34021 gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc cgtagtcttt
    34081 gctgctattg gaatcacttt tgaagaagct gaatttttca tggaagattt tcgtcaaacg
    34141 ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc aatcgaacgt
    34201 atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga aaagggaatg
    34261 catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg agagatttct
    34321 gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta cacgaactta
    34381 gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt tactcaaatt
    34441 ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt gactggttat
    34501 attacagaag gacagatcat cttgactaga gagctttaca agagtgggat tcagccacca
    34561 attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc cggaaagaca
    34621 agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca aggaaaacag
    34681 gcaaaagaat tagccgtagt tttaggtgaa tctgctttat ctgatgtaga caagatctat
    34741 gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta cacgaaccgt
    34801 tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc aagaaccgaa
    34861 ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa gtgagttctg
    34921 atggcacgat tgaatgtgaa tccgacccgc atggaattga ctcgattgaa aaaacagttg
    34981 acaactgcca caagagggca taaattatta aaagataagc aagatgaact gatgcgtcaa
    35041 ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga gtctatgaca
    35101 gaagcaatgt ctgattttgt attggcaaat gcttcgatca cggaaccttt catcgaagaa
    35161 ttatttgttt tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa tatcatgagt
    35221 gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc tgacagacac acctttggaa
    35281 tacgggtatt tgaattccag tgccgaactg gatcggtcaa tcgatcggtt tactaccctg
    35341 cttccaaaac ttttggaact gacagaaatc gaaaaaactt gtcaactgat ggcgagtgaa
    35401 atcgaaaaga cacgtcggcg agtcaatgca ttagaatata tgacgatccc tcaattagaa
    35461 gaaacgatct attatattcg tatgaagctg gaagaaaacg agcgcgcaga agtaacaagg
    35521 ttgatcaaag taaaaaatat gggtaccact gactaagtgg tatctttttt taaaagagga
    35581 aaacgatgat taaacaaagc aaacaaataa agaaacgttt gccggctgtt cagcttctcg
    35641 cattaggatt ttttctcctg attttagttg gcggcggcct acttacccta ccatttttta
    35701 gtaatagtgg gcatgggaca aattttatcg atgcgctttt cactgcaact tctgctgtct
    35761 gtgtaacggg attgacaaca ttgaatacag cagagcattg gaacagtgct ggtcaatttt
    35821 tgatcatgct tttgattgag atcggcggtc ttggttttat gatggttccg attctatgct
    35881 ttgcattgat aaagaagaaa gtcagtctca gcacacgtat tatattaaaa gaagcgctga
    35941 atctagaaga agtatcaggt gtgatgaagc tcatgcttta catcctaaaa ttggcctttg
    36001 ttatccaatt gattggtacg gttgctttat cttttgtatt tgtgcctgaa tttggctggt
    36061 tgaaaggtat ctggtacagt ttgttccatg caatctctag tttttgtaat gcagggtttg
    36121 atttgttagg agacagttta gccaatcacc agcaaaatgt ttatttgatc atggtggtat
    36181 ctgctttgat catttctggt gggttaggct tcatcgtatg gcgtgattta ctggaatatc
    36241 atcagaaaaa gaagatgacg attcattcaa aaatcgcatt aaccgttaca ggtacccttt
    36301 tattaggcgg attcgttgtg ttctttttca cagagcgcaa tggggcaacg ctagtggaag
    36361 ggaatatgtt tcaacgattg gcaaacacct tttttatgag tgtgacacct agaacggctg
    36421 gatattattc agttgattac ctccatatga gccatgcagg tctgatttta acgatgtttt
    36481 tgatgtacat cggcggaaca tctgggtcga cggccggcgg attgaaaaca actacgttag
    36541 gtgtgttgct gatccagatg cgttcgatgt tgaaaggacg tacgagagcg gaagcatttg
    36601 gccgtaccat ccgacaaggc gcagtgttgc gggcacttac tttatttttt gttacgttga
    36661 cgttgtgtgt agtcgccatc atgattttat cggtgactga aacgatacct aaaacttctg
    36721 gcatcgaata tattgctttt gaggtcttct ctgcgtttgg tacagtaggt cttacgatgg
    36781 gattgacgcc agacttgact gcaataggga aattgatcat tatagcgctt atgtacattg
    36841 gacgagttgg tgtattgact gtggtcttct ctctgatgac aaaagcacaa agaacacaat
    36901 caaaatacaa atatccagaa gaaagtgtca tgattggata atttcatctt ctgacagaaa
    36961 cctagtgatt tagtaaaata agaaaataaa ataatttttc ttgcttcttg gatactttaa
    37021 gtgcaaaatg ttcgtttatt ttttgaggca ctacccgttg ttgaacctcg ggtagtgcct
    37081 cttttttatt gaaaggaatc aataagattt gattccttta tttatcaata ggttataatt
    37141 catatttttt tattttttct tttacgatac ttagtccaga agctaaaatt tcaaggttat
    37201 tagaggatag ggaaattcga acgaatgcat ctggttgcgc acgtgtagta aagcgtctgg
    37261 aatgataaag tcggataccg ttagcaagaa agtcattctc aattatctta gggtcaatct
    37321 caggatcaac tggaagaatt cgatagtaag ggacacgatt cgttggtttt tctactttga
    37381 aaagagaatc aaaaagctga ttggctcttt ttgtaagact taatttagtt tctaagatcc
    37441 tatttgcttc atttgaatgg aggatttgag tgatgacttc cacatctaat ccagaagttt
    37501 tcacatttat attaaaaatt gcttgttcta tcttgctttg gacatgtact ggaactacaa
    37561 gataagcaac tcgcaatcca gaacaaataa attttgtcat tccagctaag tagattgttt
    37621 ggttaggtaa taaattttgg aaaggaggaa tgatcgtatg ttgatagtaa gttgttaaaa
    37681 atgaatggat atcatcttca ataacccata gaccttcttt ttcaatcact tcggctaatt
    37741 gctctcgtct tttgtgagag atttgaaacc ctataggatt attacaagaa ggcataagaa
    37801 agacaccatg aatcttcttt tttctacatt cctgaatcaa tatatcaggt cgcattcctt
    37861 gctcgtcaaa atcaattgga acgatctcta aatgatataa ctgagccaat tcaatgaaat
    37921 tagcgtatgt atatcggtct gttgctattc tatcacctgg agagaaaagt gcagaaagtg
    37981 tgatagccaa tccattttga acaccggaaa caatagatag agattctaag gacggaatga
    38041 ctccttgagt ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg cctttgggat
    38101 cttggtaatt aagcaaacca acaagatttg ggttattact agttgattga ataaacggga
    38161 caacgaattt attacattct tcaaaagaac taaccagacc aaaatcaatg atatgttcgg
    38221 ttacttcatt ggtagagatg gtgatggact caaagacatt gggagaaata aatgtgccac
    38281 ttccaatatt cgtatacagc aaacctttct caattccata tttataagcc ttgccaacgg
    38341 tagtaaaatt taaatctaaa taatcagcta gctctctttg ggagggtaac tgagtgtttt
    38401 tctgtaattt attctttaaa atatcttgtt ctaattgttg aatcaacgat cgataataag
    38461 gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg gtaattgtca taagaattaa
    38521 taggcatttt ctccactcct taaaattgta taccatacaa ttttaccatt gattgtattg
    38581 attttagaag ttaatctttt gtgaggaggg aattgtctat gaaaattatt aattttgagg
    38641 aagtagtagc ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa tgtttttttg
    38701 attatgaaag tggtaaaatc agccaaaatc ctcgattggt ggaaatttta cctgacggaa
    38761 aaggcaaaaa tatgtttgcg atgatgcctg cttatttggg ggagaacaga tattttggca
    38821 caaaagtcat taccgctttt ccggataatc atcagtatca tttaccttct catttaggag
    38881 agattttctt gtttgatgct caaaacggac tacccaaagc attgataaac gcaaatgcag
    38941 tcacctggat tcgaacagct gctgtatctg cgctggcaac agattactta gcaaatccgc
    39001 atgcagaaac acttgcttta attggagctg gtcaacaagc attgtcgcat ttgactgcga
    39061 tttctaccat tcgtcccatc actacagtgt ttgtttacga tattttagaa gaaagacgaa
    39121 atacttttat cgctaaagct aaaaaacagt atcctaaaat aacgtttata aatgcaatat
    39181 cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac gttgacctct agtaaagagg
    39241 catttcttca aaaagagtgg gtaagggaag acgcacatat caatgctgtg ggcacattta
    39301 ctcctgatac aagagagata agtacatcgt tgatggaaac ttctcaaatc tatgtagatg
    39361 attactcagc tgccttaaca gaaagcgggg acctgcttat agccatggca gaaggagatt
    39421 tagaggaatc ttgtattgtt ggtacattag gcgaattagt ttctgagaaa aaaacatttt
    39481 ataaaggaga atcgaggccg acgattttcg acgcagttgg tctagctatt gaagatcttt
    39541 gttgtgcaga atatatttat aacaaattag agaaaggggc tttaaaatga acttagatta
    39601 ttacgtagtt gatgcatttg cagatgaggt gttcaaagga aatccagcag cggtttatgt
    39661 gctagaagaa tggctgccag aaggtacgat gcaaaaaatt gccattgaaa acaatttatc
    39721 tgaaacagca tttacagtga agaaaaacca agaatttgag cttagatggt ttacgccaga
    39781 tcgagaaatc gatctttgtg gtcacgcgac attagcaaca gcatttgttt tatttaacta
    39841 ttacaaaata cctgatgaaa cgattaaatt ttctactcaa agcggtaatc tatttgtgac
    39901 aagaattaaa gattattatt acatggattt tcctagtatt atgccgaaaa aagtgccgat
    39961 attgtctgaa tatgaagaag cgattggggc taagattaaa gaagcctact tagcacgtga
    40021 tttatttttc gtattagaag atgaaaaaac ggtggctaaa ttgcagccag attttacagc
    40081 tatcaaaaat tttgaattag gaataggagt catcgtgaca gcagaaagtg tgcaaaaaga
    40141 ctttgtttct agaaccttct ttccaaaatt aacaatcaat gaagatccag tatgtggatc
    40201 agcacattct aatcttattc cttattgggc agaaagaatg aataaaacaa aattgtcagc
    40261 ttatcaaatg tcatcaaggg gcggggatct agaatgtgaa ttaaaagaag atcgtgtgat
    40321 aataggagga aaagcagttt tgttttctaa aggtacctcg tttatttgat ttctttcaaa
    40381 tgaattaaaa ataaattggt ggagagtggc aaccttatag ctacaatggt tgttaaaaaa
    40441 agacgcaaac tttttcaatt taatgaaaag tttgcgtctc attttaatta ttggtgtttg
    40501 ttttttattc aatcaagagt aaaatgcccc atactaacta tcctcggatg acttcgattt
    40561 tataaccatc agggtcaatg acaaagtagt aagaaggcgc agtgccaggt aatcctttta
    40621 agtcagtaat ctcaagaccg gcagctttat gttgctcgtg taatttttct agatcatcag
    40681 ctgctatagc gatatgacca tagccgtctc ctagatcata cgcaccatga tcatagttgt
    40741 aagtcaactc taattcatag tcatccccag gaagtgtcaa ataaacaagt gtaaatttgt
    40801 tttctggaaa atcacggcgg cggctttctt caaaaccaaa tgccttttga taaaattcga
    40861 ttgatgcttc aagatctttc actcgaacac atgtgtgagc cattttcata gattcatctc
    40921 tccttttcct tttactcaat cataacaaaa agaagtaagg aaatagttga tttgtgcttg
    40981 ttcatgagaa atttctcatg taaaatggat agaaaataaa actgttaagg tggaaactat
    41041 tcatggataa accaattttt ggtgaaaaaa agcaatttgc tgattatcaa acccgttatg
    41101 cagcatatat tgtcatagaa agagatcagc agattgcagt aatcgaggct ccaaatggtg
    41161 ctttttttct tcctggcggg gaaattgaag gagaagagac gaaagaagaa gcgattgtcc
    41221 gtgagctgat agaagaaatg ggaattgtgg cagaaatttc ttcttatctg ggagaagctg
    41281 atgagtattt ttactcgaat tatcgtgcaa cttattatta taatccagga tatttctttg
    41341 ttgctgattc ttggaagcgt ataggagaac caactgaaaa gaccaataaa atttggtggg
    41401 tgagccctca agaagcgctt gtgaaattga aacgtggtag ccaccgatgg gcagtcgaga
    41461 gatggatcga aaaaaataaa aactgaaaga aagcgagtag atcagctttc tctcagtttt
    41521 tttatttatt acagtacata ttttttgatt gcttcagcta caccgtgctc gtcgtttgat
    41581 gaagtgatga catttgctgc ttctttgaca agagaaacgg catttcccat tgctactcct
    41641 agaccagcat attcaatcat ggataagtcg ttctcgttat ctccgatagc catgatttca
    41701 tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg ccgcaccttt acttgcttct
    41761 ttatttagta cttcgaagta atagggcgca cttttgaccg ttgtgtattt atcacggaaa
    41821 gtttgcggta aacgcgcaat tgctgcatct aaaatttctg gttcgtcgat catcatcatt
    41881 ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc gatattttag cggcatcttg
    41941 actaaacttg cttcgtggat cgtataagcg ctgatatctc gattaggcgt gtagatcgct
    42001 ttgtcatcga tcgaatggag atgactgccg acacggcgtg ccatcacttc gatctctagg
    42061 aaatcttcat gtgtcagtcc gtgacgggcg atgattttac ctgatttcgt ttgttgtacg
    42121 agtgccccgt tataagtaat aacgtaatca ttatcttgaa aaaggttcaa ctcgtttaac
    42181 tgttcctgga cacctggtaa gggacggcca gtgcataaaa cgatcgaaac accttttgca
    42241 acagcagcgg caatcgcttc ttttacttct tttgttactt ctcttttctc atttaataaa
    42301 gtaccatcga tgtcgatagc gactagtttg atagacataa tttcctccaa tatattttaa
    42361 tttatttcaa tcaatgcgcc attacggata tggctggcaa attcttgata agtttcgtca
    42421 aataaatcat aatgatcacg caaggacgga tcaaccattt cttttggaaa gaaaaatcgc
    42481 tcatctcctt gttcttgtcc cgctaatgca gcaacaagct gactaacctg tgataattct
    42541 atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc gatgtctgcc ttgttctggg
    42601 cgataaaaat cataaggtag atcgtagcta gaattcacag cagtgtaata aacaggatta
    42661 tatccgacct tttctactaa caaagtgagt tcttggacta attttgaatc ccggttgcca
    42721 gaaaaagtag cagatttcag tggtttacga ttcaaaaaac gtttagctaa atcatttaaa
    42781 ataggatcag cagattcaga ccattgagta aagtaagtgc ccaagacccc atcatcaagt
    42841 ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg gcagaagtaa ttgagaatgg
    42901 agttcaaaac tgctggcatc ttcttgatac aactcgtgtg cgcgatgaag cagatgctcc
    42961 aaaattactt ccataccacg ggaaacagga tggaagtaca cttgcacata catttgatat
    43021 cggctaacga tataatcttc cactgcatgc atgccactca tggaaaaagc gatcccgcct
    43081 tcataaggac gaatcacacg taatattcgg gtaagatcaa atgttccata ttctgttcca
    43141 gtgaaataag catcccgaag caaatagtcc attcgatctg catcgatctg actagaaatc
    43201 atttggacaa cttgtgggtt ggggtaagtt ttttggatca cactagctac cttttcagga
    43261 aaaccttctt ctactcggtt aagaatctga tacacttctg tttctggaga agtgatgatc
    43321 tctacagtga tggcttcatg attcgtatgg aagatatgtt caaatgtatg agagtaagga
    43381 ccatgaccga catcatgcaa cagtgccgca cataaagtca ccagccgttc ctgatcatcc
    43441 cagcctccgt tgcctatttt ttcttttgaa aaatttcgag aaaagatatc gcagattcgt
    43501 cttgaaatct cataaacgcc taatgaatga gtaaaacggc tatgttctgc tccgtgaaaa
    43561 gtgaaagaag aagtgcctaa ttgtttaatg cgtcgtagac gttggacttc tttcgaattg
    43621 atcaaatcta agattacttg atgctggaca tggatatagt tatggactgg atcacgaaaa
    43681 actttttcca taggcagcat ttgatattta taaggggttg tcataatgta gctcctttat
    43741 gttattacgt tgttccattc tctggatttg gttgttccat tgctccagct ggatttcttt
    43801 ttttagccag tcggtagaat cctgtaaatc acttgcagaa tacagctttt caaaagcttt
    43861 tgcaaatgct tctttgactt cttttacaaa aaggggttgg tcaagcagtt cttcaagtgt
    43921 tgccattgta gcaggatcca ctgcaggata gccatttgta ccaaagtctt cttttaaagc
    43981 agattgataa aatcgcttca tgacttgccc gcgtttttcc tgattaccgt tgacacttaa
    44041 gtacatcata acagaaatcc catttttgat ccgacgctga gcgataccgg caaatttttt
    44101 gccatggatg cttaaatcat aagttccagg acaataagaa tgggtgattt cgaatgcttg
    44161 tatcggtgcc tttggaaaag cgtatttagt caataggagc atttgctcgt aagcgtcatc
    44221 tatggttatt ttttttgtgt cactttgggg gaaaatcaaa gaaatattca aaattccttc
    44281 atctgatacg atccctaatc cacctgaatt tctgatcact ggagcgtaac ctgcatcttt
    44341 cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca tccttcatac ctaagatgac
    44401 tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg tgttctttag aatattccgt
    44461 taatatatct gttaatgcaa aaggggaaaa gtcgtttttt tgataaatgt gctgatctag
    44521 tcgaatcgca gggttcattc aaccggctcc tttgtataaa ttcatatttt ctacaatatt
    44581 atactctaaa atcaaagagt tgttttggaa aaaaggtttt ctgtaaaaat ggacaagttg
    44641 gttgacaaaa caaaagaaac taggaacaat gtagctatga gaagtaaagg agaatgaata
    44701 atggaattat cacaaggaac accgatttca atcaaattgc gtacaaaagt aaaacagcaa
    44761 ggggaagtgc aagattttta ttttgatttg aaagggcaaa tggtgacaat tggcgatacg
    44821 ctgtacatac gttataaaga gattcaggag gagacgggcg atgagattcc tgtaacgatc
    44881 aaattagttc cagatggtca tgtacagctg atccgtgcag gagaaatgcg gatgcgtttg
    44941 aaatttggct ataaagaacg gttagaaaca tcctatcgaa caccatacgg tatgatccag
    45001 attgctactt ttacgaaaga actgcatgtt agtttgaaag accgtcccac aggagggaag
    45061 gttcgaatcg attatgatct gttcatggga ccggaaaaaa tcggcgaata ttatttgacc
    45121 ttggatttta cagcgtaaat ataaaagaaa agtttgattt tttgtgtctg attttgtatg
    45181 ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa ttagtgtatt tgaaggtgca
    45241 aacaaaagcg aattatcaat gatcgaagta gcacacgcga tcttagaaca acgtggcgat
    45301 gtcatggatt tttctgattt agctaaccag atccaaaact atcttgggaa atcagacagt
    45361 gaaattcgtg attcactcgc acaattttat actgacttga atattgacgg cagctttatc
    45421 tcattagggg ataatcgttg gggcttgcgt tcatggtatg ctatcgattc gatcgatgaa
    45481 gaagtaaacc atggaatgga cgaagaggat gaagatactc cacgtcgccg taaacgcaag
    45541 aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg attacaatga cgatgaccca
    45601 gaagatacag atctgacaga agaagatgac gatgatctgt ttgatgacga tgatgatgaa
    45661 gatgaagaaa tcgctgctta taattctgac ttgcaagaaa tcggagcaga tgacccgaca
    45721 gatgatgaag aagacttgcc aggaaatatc gaagaagatt taagtatcat agacgaagac
    45781 gatgatgatg aagattatga agaggaagaa ttttctgact ctgaaaaaga ataaacatta
    45841 ttttcttacg agaggttgtg acagagacga tcagcctatc agaataagga ggtgtgacac
    45901 gatttttgtc acaccttctt ttttgtatat aaaaatccca agtaaataca taaaaaataa
    45961 aaaaattgaa acttttagcg caccttgtac gtcatattaa gtgaggcaga aaggagtgga
    46021 gaatgatttt tactggatat aagaaaaaac ggcagatcga aaaataccgt cacctgctga
    46081 atggactcat cgaaactgat tatcaaaaaa tgttccgtat cacgatgaat tacgtacatg
    46141 ataaagaaga ggccttagat gtgatgcaag atagttttca taaagccctg aactattttg
    46201 aaaaagaaaa agagatcgag cattttagtg catggtttta tcggatattg atccgcacgg
    46261 cattagatag ttggcgcaga caaaaaagga cgcctgtgga cttatttgaa attcaagata
    46321 tcccccaaca gttaccttta gaaacttccg ttgcagagct ccatatgatt ttagataaaa
    46381 tagacagtcc ggaaaaagag attctgatcc tctacttttt tgaaggattt cgtttgaaag
    46441 aaattgcgac tatcttggat atgaatgaaa acacagttaa aacgaaaatg taccgctcat
    46501 tgaatgcttt acgtcaaatc ctagaatgaa ggagagaaga gtgccatgta ttcccaaaaa
    46561 gaaaatttaa aaaaactaaa gaaaacttat aaagaacaag caattcccaa agggcttaaa
    46621 gaggagatcc gccagcgttt tattatcgaa gagcaatcat ttataaaagc aaagcggaga
    46681 aagagacgtc tccaagggtt cggagtatct ttggctatcg taatgattac aacaagtagc
    46741 cttcttttta atagtcaagt tcgaagcttt gcagaagatt tacccatctt aggatcagtc
    46801 attgaattga tcttaggcga acgctttact gatcgatctg aaaaaatcga tattcaggta
    46861 ccgaagatca atacacaaaa tgagaaagaa aataaaacaa ttcatggatt aaatcaaaaa
    46921 tactttcgcg aaggacaagc agattttgaa aaagctgaaa aagaatatgg aaattttgaa
    46981 acggatcatt accaagtttt gggagattat cagaaaatcg tagacgataa ccggtttctc
    47041 gtgattgaaa ggcagatcac ccaaacagca gcagatagcc atgttgaaaa aagatacgac
    47101 acaatcgaca agaaaaacag tgtccagctc tcacttccac ttttattcaa agatgacacc
    47161 tatctatctg ttttgacaaa agaagtcaaa cggcagatgg ccgaacaagt caaggaagat
    47221 ccatctaagt actattggac tgagcaagat attcaagaag ggacgataga aaaaccaaca
    47281 cttgttacgc ctacacgaag tttttatttg aacaaagagc atcaattagt tttgactttt
    47341 tctcaatacg aaattgctcc tggttatatg ggtacaccag aatttgtcat tcctaagtcc
    47401 gtgacaaaaa cgattttggc atcggaagat tatttggatc attagacgag aagtagttgc
    47461 gactagcagt ttcaattttc aagtaagtac aagtaaataa taagaagaga aaaagagaaa
    47521 gaagagatat agtgaaaaga aattatttag ttatcagtat tattgcttta tccgtgcttt
    47581 tgtttttcga aattacttcg gtggcctcct tgatcaccga agtaaaagga caaacacact
    47641 cttcggagca ttcgtcacaa tctttcaaac aatcagaaca atcaaccatt aaaagccaat
    47701 caactgaaac agctaacgtc aaagaagcca ttcaagaatc agcggaaaag gtacaaagtc
    47761 agtcatcaca atcaacacaa gacagtacaa aaacgagccg agatccggct gtcaaaagcg
    47821 tcgcaatcag ctttgatgat ggaccaggag cgaccacaac accgcagctt ctgcgaatct
    47881 taaaagaaaa aaacgtgcat gccacttttt tcgtcttagg agaaaatacg gcgcagcatc
    47941 ctgaaatcgt gaagcagaca gctgaagcag ggcacgagat tggcaatcac acatacgatc
    48001 atcagaatct ggccactctt tctgcccagt cgatgacgga ggaagtgacg aaagcagaca
    48061 ctgagatcaa aaaagtcact ggcaaaaccc ccacttttgt tcgtccgccc tatggttctg
    48121 tcacgaatgt aggagcaacg atcatccagc gaccgatcat cgaatggtct gttgattcag
    48181 aagattggaa gacaagaaat cctgatttga ttttgcagaa gatccaagca acagtatacg
    48241 atggggcaat tattttattc catgatatct atcctgaaac gattcgtgcc gttcctcaag
    48301 tgatcgatta tctgaaagag caagggtatc gcatcacgac agttggggat ttattagggc
    48361 atccaacggc tgtcgagaac tattatggca gaaacgatca tcggccagtt cagtaagtga
    48421 gacaagtatc tttaataaat aacacatttt aataagtagt tgaacacata actaccttac
    48481 ttgcaaaggc ttaacaatat ctatacaggt acaacaaaaa actgtccacc aattttcttt
    48541 ggcggacagt tttcacttaa aaataaagaa gcaactattc gtaaattatg ctaaaatagc
    48601 agtagaacgc gtcctgaggg aatcgaaccc ccgtctcaag aaccggaatc ttacgtgata
    48661 tccactacac taaggacgca agcactggga taattgtaac taaacagata aaaaattaca
    48721 aggggattgg acaaaaaaat gaaatttgag caaaattttt cacaaaaaca gcagcagaca
    48781 cagaaactag ccatgacgca acagttgcaa cagtccatcc aagttttaca atataacagt
    48841 gaggagctgt tggcattcgt tgagaatcaa gcaatggaaa atccattggt ggaagttgtt
    48901 gagccggaat ggcagccaga ttacataaaa gcttcttcat cttcttatga aggagaagag
    48961 acaaattacc taaatcagat tcctgataca aaagggtctt tatttgattc attgatcgaa
    49021 caagtccatt tgaattaccg agatactttt ttgaggaaaa tcgttcttta tcttgtagaa
    49081 tacatcgatt tgaacggatt tttgacgatt agtttagaag aagccggaaa agagattgga
    49141 gctacgccta tccaaatgct cgatgcgttg actctgattc agcaattaga accagctgga
    49201 gtaggtgcac gtagtttgca agaatgtctc atgcttcaaa cagaacgaga tgattatgcg
    49261 ccggaattag cttatatcgt gttggaagaa tgttttgagg aactggtgga gcgaaaatgg
    49321 aaagaaatcg cacaacgttt ttctgttgat ctacatgctg ttcagcagat attcgactat
    49381 ttacaaacgt tgagtccaag ccctggccgg attttcgacc gtagcagcga attgtacatt
    49441 attcctgatg tccgggtatt agtggacgac gataaaaatg tacaagttat ctcaaaccga
    49501 aaaaatcagc caaatatacg ctttcaagag agttacttca aacaaatgtc acaacaagca
    49561 gataaagaaa ctgaaaatta tttaaaagaa cggaaacagg aattcgaatg gttgaagaaa
    49621 acgatcctac aacgtgggga tacaattttg cgggttgctc aagtcattgt ttcccggcaa
    49681 aaagctttct ttatcgacaa ggaacgtcca atcaagcctc ttacgttaaa ggaagttgca
    49741 actgagatag atgtgcatga atccacggtc agtcgtgcag tcaacgggaa atatttggaa
    49801 acagacttcg gtgtatttga attaaaaaaa tttttcacta cacgaattcc tacgaatagt
    49861 acagaacaga cagaagacct ttcagcagat acagcgaaga aaaagctgca ggaattagtc
    49921 gatcaagaag acaaaaacaa gccgttgtct gaccagaaat tggttgaatt gctgaaaaaa
    49981 gatgagatag cgatctcaag acgaacagta gcaaaatatc gtgatttatt aggaatcccg
    50041 agttcatcaa aaagaaaacg ttatgataac taatcagtca aaagactaga aaaagtattt
    50101 cactttttct agtcttttta gttgaaaaac gagagcaaaa ctgttagact tacagtcgtg
    50161 aggttgagat ttccctttat tgatgtgaaa ttcgaatagt taatttcttt ttttatcgta
    50221 tatgggtcgt tttaagtcta cattggacgt aaagtgtcca actaaggaga tcgctatgct
    50281 ggatgaaata aaaatgattg aatctgttgc accagatatt atcgaagtgt tgcaagaacg
    50341 ctataaaatt ttacgaaata tctattggat gcagccgatt gggcgtcgaa gtttatcgga
    50401 aagtatgggg atcacagaac gagtcttacg aacagaaaca gaccttttga aaaatctgaa
    50461 tttgatcgac acctcaaaaa gcggtatgac gctgacaact aagggcgaag aagtttatca
    50521 aagccttgaa aacttcatgg atcagctttt aggtacgcat caaaccgaac aacagttggc
    50581 agaatatttt ggtaaccaac gctgtatcgt tgtttcagga aacagcgaag agcagaccaa
    50641 agtggctgat gcattcggcg aagcgctgag cgaagcctta gaccgactac ttccagaagg
    50701 cgagaatatc atcgctgtga tgggaggaac tactatggct gtggtagccg aacaattgtc
    50761 taatctggaa aataagaaac ggcataacct gtttgtccct gcaagaggcg ggattggtga
    50821 agcgataact gtacaggcaa attctgtcag tgcaagaatg gctgctaaag caaacggtaa
    50881 ccaccgcgca ctttatgttc cggaacaatt aagtctggca acttacaatt ctttactcaa
    50941 tgagccatct atccaagaag tcttaaactt gattagtgaa gcgaattgtg ttattcatag
    51001 tattgggcgt gcattacaca tggcagcacg tcgtaagatg actgaaaaag aattagtcat
    51061 gctgaaacaa gcaaatgctg ttgcggaatc ttttggctac tttttcaatg aaaaaggtga
    51121 agtcgtttat aaggtcccta gaatcggcat agaattgcaa gatttggaaa aagtcccgat
    51181 catcatggca atcgcgggcg gaaaatcaaa agctaaagca attcgagctt atatgaaaaa
    51241 tgcaccaaaa caaacgtggc tcatcactga tgaagctgct gcaaatgaga ttttaaaagg
    51301 ggtaaccctt taaaataaat atttttttga ttttcataag gaggaaatct taaatgacag
    51361 ttaaagtagg tattaatggt tttggacgta ttggacgtct agcattccgt cgtatccaag
    51421 atgtagatgg aatcgaagta gtagcaatca acgacttgac agatgctaaa atgttggcac
    51481 acttgttaaa atacgacaca actcaaggac gtttcaacgg aacagttgaa gttcatgaag
    51541 gttcattcaa cgtaaacgga aaagaagtta aagtattagc taaccgcaac ccagaagaat
    51601 taccatgggg cgaactaggc gtagatatcg ttctagaatg tactggtttc ttcacttcta
    51661 aatcagcagc tgaaaaacat ttaacagctg gtgctaaacg cgttgttatc tctgctcctg
    51721 gcggaaacga tgttccaaca atcgtttata acacaaacca cgaaacatta actggtaaag
    51781 aaacagttat ctcaggtgct tcatgtacta ctaactgttt agctcctatg gctaaaacat
    51841 tgaacgacaa atttggtgtt gttgaaggat taatgacaac tatccacgct tacacaggtg
    51901 accaaatgac tctagacgga cctcatccta aaggtgactt ccgccgtgca cgcgctgctg
    51961 ctgcaaacat cgttcctaac tcaacaggtg ctgctaaagc tatcggttta gtaattcccg
    52021 aattgaacgg taaattagac ggagctgctc aacgtgttcc tgtaccaaca ggttcattaa
    52081 cagaattagt aacagttctt gaaaaagaag taactgttga cgaaatcaat gcagcaatga
    52141 aagaagcttc aaacgaatct tacggataca acacagacga aatcgtttct tcagatatcg
    52201 ttggtatgac ttacggttca ttatttgatg ctacacaaac taaagtaatg acagttggcg
    52261 acaaacaatt agttaaaact gttgcttggt acgacaacga aatgtcatac actgcacaat
    52321 tagtacgtac tttagaatac ttcgctaact tataagatag acaaacgtac aagtgtaaat
    52381 tatagacatg tacatttaaa agcggggaag caatcgcgct tcttccgctt tttttgataa
    52441 taagtttatt
//
LOCUS       DACDRK010000011        47419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDRK010000011 DACDRK010000000
VERSION     DACDRK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.011"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            128..1396
                     /gene="rseP"
                     /locus_tag="IWJ77_000752"
     CDS             128..1396
                     /gene="rseP"
                     /locus_tag="IWJ77_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9452395.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            1464..3173
                     /locus_tag="IWJ77_000753"
     CDS             1464..3173
                     /locus_tag="IWJ77_000753"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9452396.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            3385..7743
                     /locus_tag="IWJ77_000754"
     CDS             3385..7743
                     /locus_tag="IWJ77_000754"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9452397.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            7888..8361
                     /gene="rimP"
                     /locus_tag="IWJ77_000755"
     CDS             7888..8361
                     /gene="rimP"
                     /locus_tag="IWJ77_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9452398.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            8384..9559
                     /gene="nusA"
                     /locus_tag="IWJ77_000756"
     CDS             8384..9559
                     /gene="nusA"
                     /locus_tag="IWJ77_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9452399.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            9581..9874
                     /locus_tag="IWJ77_000757"
     CDS             9581..9874
                     /locus_tag="IWJ77_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9452400.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            9871..10182
                     /locus_tag="IWJ77_000758"
     CDS             9871..10182
                     /locus_tag="IWJ77_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9452401.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            10195..12501
                     /gene="infB"
                     /locus_tag="IWJ77_000759"
     CDS             10195..12501
                     /gene="infB"
                     /locus_tag="IWJ77_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9452402.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            12528..12875
                     /gene="rbfA"
                     /locus_tag="IWJ77_000760"
     CDS             12528..12875
                     /gene="rbfA"
                     /locus_tag="IWJ77_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9452403.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(13032..13205)
                     /locus_tag="IWJ77_000761"
     CDS             complement(13032..13205)
                     /locus_tag="IWJ77_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9452404.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            13653..16015
                     /locus_tag="IWJ77_000762"
                     /pseudo
     CDS             13653..16015
                     /locus_tag="IWJ77_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            16383..17306
                     /gene="truB"
                     /locus_tag="IWJ77_000763"
     CDS             16383..17306
                     /gene="truB"
                     /locus_tag="IWJ77_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9452405.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            17310..18251
                     /gene="ribF"
                     /locus_tag="IWJ77_000764"
     CDS             17310..18251
                     /gene="ribF"
                     /locus_tag="IWJ77_000764"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9452406.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            18603..19388
                     /locus_tag="IWJ77_000765"
     CDS             18603..19388
                     /locus_tag="IWJ77_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9452407.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            19401..20252
                     /locus_tag="IWJ77_000766"
     CDS             19401..20252
                     /locus_tag="IWJ77_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9452408.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(20376..20828)
                     /locus_tag="IWJ77_000767"
     CDS             complement(20376..20828)
                     /locus_tag="IWJ77_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9452409.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(21293..21661)
                     /locus_tag="IWJ77_000768"
     CDS             complement(21293..21661)
                     /locus_tag="IWJ77_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9452410.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            21811..22293
                     /locus_tag="IWJ77_000769"
     CDS             21811..22293
                     /locus_tag="IWJ77_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9452411.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            22330..23340
                     /locus_tag="IWJ77_000770"
     CDS             22330..23340
                     /locus_tag="IWJ77_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9452412.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(23455..24168)
                     /locus_tag="IWJ77_000771"
     CDS             complement(23455..24168)
                     /locus_tag="IWJ77_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452413.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24358..24948)
                     /locus_tag="IWJ77_000772"
     CDS             complement(24358..24948)
                     /locus_tag="IWJ77_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9452414.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            25432..25659
                     /locus_tag="IWJ77_000773"
     CDS             25432..25659
                     /locus_tag="IWJ77_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9452415.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            25675..26841
                     /locus_tag="IWJ77_000774"
     CDS             25675..26841
                     /locus_tag="IWJ77_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9452416.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            27075..28118
                     /gene="hrcA"
                     /locus_tag="IWJ77_000775"
     CDS             27075..28118
                     /gene="hrcA"
                     /locus_tag="IWJ77_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9452417.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            28212..28775
                     /gene="grpE"
                     /locus_tag="IWJ77_000776"
     CDS             28212..28775
                     /gene="grpE"
                     /locus_tag="IWJ77_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9452418.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            28826..30655
                     /gene="dnaK"
                     /locus_tag="IWJ77_000777"
     CDS             28826..30655
                     /gene="dnaK"
                     /locus_tag="IWJ77_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9452419.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            30806..31972
                     /gene="dnaJ"
                     /locus_tag="IWJ77_000778"
     CDS             30806..31972
                     /gene="dnaJ"
                     /locus_tag="IWJ77_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9452420.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(32054..32719)
                     /locus_tag="IWJ77_000779"
     CDS             complement(32054..32719)
                     /locus_tag="IWJ77_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP9452421.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            32695..32868
                     /locus_tag="IWJ77_000780"
     CDS             32695..32868
                     /locus_tag="IWJ77_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9452422.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(32913..33650)
                     /locus_tag="IWJ77_000781"
     CDS             complement(32913..33650)
                     /locus_tag="IWJ77_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9452423.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(33652..35154)
                     /locus_tag="IWJ77_000782"
     CDS             complement(33652..35154)
                     /locus_tag="IWJ77_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9452424.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            35413..37407
                     /gene="uvrB"
                     /locus_tag="IWJ77_000783"
     CDS             35413..37407
                     /gene="uvrB"
                     /locus_tag="IWJ77_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9452425.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            37417..40233
                     /gene="uvrA"
                     /locus_tag="IWJ77_000784"
     CDS             37417..40233
                     /gene="uvrA"
                     /locus_tag="IWJ77_000784"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9452426.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            40496..41761
                     /locus_tag="IWJ77_000785"
     CDS             40496..41761
                     /locus_tag="IWJ77_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9452427.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            41765..42496
                     /locus_tag="IWJ77_000786"
     CDS             41765..42496
                     /locus_tag="IWJ77_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9452428.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            42631..43515
                     /gene="rapZ"
                     /locus_tag="IWJ77_000787"
     CDS             42631..43515
                     /gene="rapZ"
                     /locus_tag="IWJ77_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9452429.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            43512..44510
                     /locus_tag="IWJ77_000788"
     CDS             43512..44510
                     /locus_tag="IWJ77_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9452430.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            44544..45479
                     /gene="whiA"
                     /locus_tag="IWJ77_000789"
     CDS             44544..45479
                     /gene="whiA"
                     /locus_tag="IWJ77_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9452431.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            46022..46672
                     /locus_tag="IWJ77_000790"
     CDS             46022..46672
                     /locus_tag="IWJ77_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452432.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            46859..>47419
                     /locus_tag="IWJ77_000791"
     CDS             46859..>47419
                     /locus_tag="IWJ77_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP9452433.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        15915 a         8147 c        10181 g        13176 t
ORIGIN      
        1 aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg gtatactatg
       61 gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag taaaggagaa
      121 aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag tgattgttca
      181 tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgttcgcg aatttgctat
      241 cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat atacgcttcg
      301 cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg aaacagaaat
      361 ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag agaagataaa
      421 tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc gttatgattt
      481 agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag tagtaaccta
      541 ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca gaattgcgcc
      601 aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca actttgctgg
      661 acccatgaat aactttattt tggcaatcct gttattcatc attctggcat ttatgcaagg
      721 tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg gagctgcggc
      781 agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag aaatccattc
      841 atggaatgat ctgacaacag tcattacgaa aaatccggat aaaacattag actttaagat
      901 cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag agtcaaatgg
      961 cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta tggataagat
     1021 cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag ccttaggatc
     1081 actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt accaattgtc
     1141 ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc ttctttcaat
     1201 gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga aattagtctt
     1261 gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag gaatattgac
     1321 attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga acgatattca
     1381 acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga taactatttt
     1441 gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca acattacgcg
     1501 aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct ggatatatcc
     1561 gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta ttggaaaaac
     1621 tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg ctgatgcctg
     1681 ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat ggaccaaact
     1741 tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact cacgaagaaa
     1801 cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct ttaaatcttt
     1861 atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg cttcgcggaa
     1921 gagagtttat catgaaagat gcgtattctt tccatgcaac ggaagaaagt ttggacgaga
     1981 catataaaga ttacgaaaaa gcctacaccg aagtattcaa gcgttgtggc ttagaatttc
     2041 gctcgattat cggagacggt ggcgcaatgg gcgggaaaga ctcaaaagaa ttcatggcga
     2101 tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat gctgcaaact
     2161 tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag cttgaattgg
     2221 agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc ttttctgttg
     2281 aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct gtccttgttt
     2341 tggtacgcgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta tctgctgact
     2401 ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag tttggttctg
     2461 tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat gtccaagatt
     2521 tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat gtgaatccag
     2581 aacgcgattt cactgtactt tcttatgaag atcttcgttt tgtacaagaa ggagatcctt
     2641 caccagacaa caatggtgtt cttgccttta ctcgaggaat cgaaatcgga catatcttca
     2701 aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa aatggacgag
     2761 aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta tcagcaatcg
     2821 tagaacaaca ttcagatgaa caaggaatca actggccaaa aggaattgcg ccatttgatt
     2881 tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aaacctgacg aatgaactag
     2941 aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat gaacgcgcag
     3001 gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact gtcggtaaaa
     3061 aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg gttgaagtac
     3121 gtaaagaaga attggtgaat actttaccaa tcctcttgaa ccaaggcgaa taataagcgt
     3181 taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga tgattcgctt
     3241 ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa actcagaaaa
     3301 aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa ttgcattgtt
     3361 tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga aaaactgttg
     3421 gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc tggtgaaatg
     3481 gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctgca tttttccaaa
     3541 attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc ttttaaagat
     3601 attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga acagctcttg
     3661 caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact agcacagcaa
     3721 gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc aatcgacagt
     3781 acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga gttgttcgtc
     3841 agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca agcggaacga
     3901 gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat gaagcaagca
     3961 gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca atcgccagct
     4021 cttgaaggac ccatccacat ccagctagga cgaaatattc cagccgatga accaacgaca
     4081 ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta cgtattcgac
     4141 aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat cacagattat
     4201 acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca agtatttgat
     4261 gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga tacgtttgtc
     4321 cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc tcgcaaagat
     4381 tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat gagtacgatg
     4441 gatgcaacaa acagcatctc tgatcttgtg gcacaagcag ggaaatgggg acaccgagct
     4501 atcgccatta cagaccacgg tggtgcgcaa gcctttccgg aagcacatag tgctggaaaa
     4561 aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga tggtgtccca
     4621 attgcttata atgatgcaca tgaagcattg agtgaagcca catatgtcgt atttgacgtg
     4681 gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc agtcaaaatg
     4741 tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca tcctttgtca
     4801 cgaacaacgg tagatctgac tggtatcacg gatggaatgg ttcgaggatc aaagtcggaa
     4861 gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt agcccacaac
     4921 gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat tcctgaagcg
     4981 gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca attcaaacgt
     5041 ttcggattag gtgtgttatc taaaaaattt ggtgtcagct tggaacagca tcaccgagca
     5101 atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga ggcaatggac
     5161 aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg agattcttac
     5221 aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg actgaaagat
     5281 ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc acgaatacca
     5341 cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg tgataaggga
     5401 gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg agcaaaattc
     5461 tacgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga gcaagaattg
     5521 gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat cggaaaaagt
     5581 ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga agatgccatt
     5641 taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg tcatagtttg
     5701 ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt tttaggggaa
     5761 gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat ctgcgaagaa
     5821 gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga agatgaaata
     5881 tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc tgagatcatt
     5941 caaaaacgat taaaaaaaga gttgaattct attaatggta atggcttctc ggttatttat
     6001 ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt aggttcacgt
     6061 ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt taatccgtta
     6121 gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga tggtacgtat
     6181 ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg cttaaacaaa
     6241 gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa agtgcccgat
     6301 attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac gaaagtattg
     6361 tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga caaaacagca
     6421 tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag tgcagaagtc
     6481 gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca tccagggggg
     6541 atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca atatccagca
     6601 gacgatcaaa attcggaatg gaaaacgact cattttgatt ttcactcgat tcacgataat
     6661 gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat gcttcaagat
     6721 ttatcaggga tcgatccaca aacgatccct acggatgacc cggaagtgat gcggatattt
     6781 gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac agggacatta
     6841 ggtattccag aattcggcac tcgttttgtt cgagggatgc tagaagaaac gcatcctaca
     6901 acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt atggttagga
     6961 aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat cggctgtcgt
     7021 gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc atttaaaatc
     7081 atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg agaaacctat
     7141 ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg ttcaaaaatc
     7201 aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct tcgtgtcgct
     7261 tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt acgtgcagac
     7321 gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc aatgaaagaa
     7381 atcacggaca aaggattaga tgcttccgta aaagaaaaga atcaactgac agtgctggag
     7441 ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt gtataaatcg
     7501 gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag agctgtccca
     7561 agtttgggaa ccaacgtggc aaaacaaatc gttgaagcaa gaaaagacgg tccattttta
     7621 tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga atatatgaac
     7681 gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt cgatatgcta
     7741 taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata gttcttatag
     7801 gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat ggaattacgc
     7861 acttaatttt ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt cacagaaatg
     7921 gtgacaccga ttttagatga acaaaatttt gaacttgtcg aagtcgaatt tgtaaaagaa
     7981 ggaaaaagct ggttcttacg agtatttatt gacaaagaag gcggtatcga tatcgaagaa
     8041 tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg cagcagatcc agatccgatt
     8101 cctcaagctt atttcctgga ggtatcctcc ccgggagcag agcggccatt gaaaaaagaa
     8161 agcgactatg aaaaggcact tggtgattat gtccatgtct ccttatacca accagtagat
     8221 ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg ctgaacagct tacattgaaa
     8281 atacgcataa aaacacgaga aaaagaattg acttttgacc gaaaaaatat tgcaaaagct
     8341 cgcttagcta tccaatttta atcacggaga ggaatcagaa aaaatgagta aagaaatgtt
     8401 aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg tgatcgatgc
     8461 tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac aaaacgtcga
     8521 agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag aagtaacgga
     8581 agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa tcaatccagc
     8641 atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg ggcggatcgc
     8701 tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa gaacgattat
     8761 ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg aacgccagga
     8821 taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta aacaagatca
     8881 aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt cccgagtaga
     8941 aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc tattgcgccg
     9001 cttatttgag caagaagtac cagaagtcta tgatggaatc gttgaaattg tcagcatcgc
     9061 ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa atatcgatgc
     9121 agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca atgaattaaa
     9181 aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca ttgcaaatgc
     9241 attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa aggcctgcac
     9301 agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac aaaacgcacg
     9361 tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg acatgacgga
     9421 attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg aagcaatcgt
     9481 tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg aaacggtcga
     9541 agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa ggaaaattcc
     9601 tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg tccgaatcac
     9661 ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg gacgaggagc
     9721 gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa ttctcgatcg
     9781 agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact atgtcactca
     9841 ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca aaaagccatg
     9901 aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga actgaccatt
     9961 gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc aagcgagaat
    10021 acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt tgagctattt
    10081 tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg aataatggat
    10141 gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga ttgcatgggc
    10201 aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga cgtcgtggaa
    10261 aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac tagcaatgat
    10321 gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc tgcacaacaa
    10381 gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga aacaacgaac
    10441 aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg gagcggtcaa
    10501 gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac cagcagccaa
    10561 aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa caacaaacca
    10621 aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa taaaaaaggt
    10681 aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa attccgtgaa
    10741 ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc gaaaaaaatt
    10801 catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat ggtcaatcaa
    10861 aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg catggaacca
    10921 caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc agaagaagtc
    10981 gatccagata aattagtctc acgtccgcca gtcgtaacga ttatgggaca cgtagaccat
    11041 gggaaaacaa ctttattgga tacattacgc cattcacgtg taacaagcgg cgaagccggc
    11101 ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc aatcactttc
    11161 ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc aagtatcaca
    11221 gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac agttgaagca
    11281 atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa aattgataaa
    11341 ccaggtgcaa accctcaaca tgttatgcaa gaattaagtg agtatgagtt aattcctgaa
    11401 gcatggggtg gagaaacgat ctttgttgag atctcagcga aattcggcca aaacatcgaa
    11461 gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc agatccaaca
    11521 caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg tcctgtgtca
    11581 accttacttg tccaagaagg aacacttcat gttggtgacc caatcgttgt cggtaatact
    11641 tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga agcaggacct
    11701 gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga ccgtttcgta
    11761 gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcgaa acgtgcatta
    11821 ttagaaaacc gtgctgccac aagccgtgtg actttggata acttatttga aagcctgaaa
    11881 gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg ttcagcagaa
    11941 gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa aatcgtccat
    12001 tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag caacgctatt
    12061 atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga tactgaagaa
    12121 gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga aactgcgatg
    12181 aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat cgttcgtgaa
    12241 accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga aggatatatt
    12301 cgtagagata gcggtgtccg tgtaatccgt gacggaatcg taatctatga aggccaactt
    12361 gcaagtctga aacgattcaa agatgacgtg aaagaagtca aaatgggtta tgaatgtggt
    12421 gcaatgattg aaaaattcaa tgacatcaaa gtagatgacg taattgaagg atttatcatg
    12481 gaagaagtca aaaatgacta aaaactgata aaaggaggcc aatcattatg gctaactacc
    12541 gtgaccgccg agtcggtcaa gaaatcttaa aagaagtcaa tgatattttg cgcaaaaagg
    12601 tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt gaaagttacg ggggacttac
    12661 agcaagcaac gatctattac agcattttat ctgacttagc atcagataag aaaacagcac
    12721 aagatggatt gaataaagca tcaggattga ttcgtcgaga gttagggcac aatctaagta
    12781 tttataagac acctgaattg ttctttgaac ttgatgaatc agttgtctat ggcaaccata
    12841 tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa aactcccgca aatcctgtga
    12901 aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag agttccatga
    12961 ctttagtctt gcggcgtgtc agctaaccaa aaaagagaaa aagaaaagta aaaaattcga
    13021 ctttcttttt tctagtagta tataaatttt tgcatataga taaagatcaa gataccgata
    13081 acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt aactagaaga
    13141 tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt taaaagaaaa
    13201 atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca cgatccaaat
    13261 tttggataat ccctgtagaa attagcatag tagctgggat accaattcac atattgacaa
    13321 agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct cgatccagcc
    13381 ccagttttcc agcaatgtgt taactggccc agcgtcattc agcaaattat gcatgatgag
    13441 gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg atcaatgtaa
    13501 aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt ggctagatat
    13561 aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta tctgcgctag
    13621 aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa gaatactttg
    13681 ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa ctttatctta
    13741 aagataaccc cttactgaga cgtacactga aacccgaaga tgtcaaaaaa catccaatcg
    13801 gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac cgagtcatca
    13861 ataaatatga tttgaacatg ttttatattg aagggccagg acacggcgga caagtcatgg
    13921 tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta acggaagatg
    13981 agacaggaat gcaaaaatta ttcaaacgat tttcatttcc tggaggcatt gcttctcatg
    14041 cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac tctctttcac
    14101 atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg attggtgatg
    14161 gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt atcaatccag
    14221 tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata gccaacccaa
    14281 cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga cttggctggg
    14341 aacctttctt tattgaagga aatgattcag aaaaattaaa tcctgttatg gctgaaaaaa
    14401 tggatcaagc aatcgagaaa attaaatcca tccaaaaaga agctcgcctg aagacagcaa
    14461 cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa ggatggaccg
    14521 gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac caagtaccga
    14581 ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg ctgaaatcct
    14641 atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc agagaaatcg
    14701 cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga atcgatccaa
    14761 gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa ccaggctccg
    14821 ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt gagaaaaatc
    14881 cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg aatcaagttt
    14941 tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat gaatggcttt
    15001 cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa gggttcttag
    15061 aagggtacgt tctgactgga cggcatggtt ttttgcaagt tacgaatcat tcttgcgagt
    15121 ggttgactct atgttgactc agcacttcaa atggatgcgg aaatcacatg acctttcttg
    15181 gcgaaatgat tatccttcgc taaatctcat tgcaagctca acagtcttcc agcaagatca
    15241 caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa agaaggcgga
    15301 gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca tggataaagc
    15361 attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc gtgctcaatt
    15421 ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca ttgattgggc
    15481 ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa cggaatctaa
    15541 cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac taaaaattcg
    15601 gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc cgcgtgggtt
    15661 gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat ttgctttcca
    15721 cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc atttaatgat
    15781 tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg tagtaaatga
    15841 acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa gtcaagcaga
    15901 agattttgcg aaaaaaatgg atcagaaatt acaagaacat caaaactata ttcgtgaaaa
    15961 tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc aataaaatga
    16021 taaaatcgat cccgcaaaat agaatttttt acattgatta agaaactgaa aaaataaata
    16081 aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt ttttcgtttt tgggaagtga
    16141 aaaccaaact atttacctgt aagccaccca taaatccaat caaatggtgc aaagaaaaaa
    16201 tcttccattc tattccctcc tttaaaaaag cataacatga tttctattta cgtgagcatg
    16261 aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt cattatgatg tcaaattcgt
    16321 cagtaattga tgcaatgtgt tataataatt cagttaaatc aaaaatgaaa tggagaaaag
    16381 acatggacgg gttattacct ttatggaaag aacgcggaat gacaagtcat gattgcgtat
    16441 tcaaattaag aaaaatctta catacgaaaa aaatcggaca tgggggtacg cttgacccag
    16501 acgtagacgg tgtgttgccc atttgtattg gaaaagccac aaaagttatc gaatatttga
    16561 cagattctgg gaaaatctat gtaggcgaga taactctagg tttcagtaca acgacggaag
    16621 atcgatccgg agaacgggta gaaacaaaac cagtagatat gtctttatct gaaaaaaaaa
    16681 tagatgaagt gatggcttca tttattggtg agatcaccca gataccacca atgtactcag
    16741 cggtaaaagt caacggtaag cggttatatg aatatgcccg caataatgaa acagtggagc
    16801 gtcctgttag aaaggctcag atttaccgtt ttatccggac aagtgaattg acaagagacg
    16861 aagtgacagg aaccatttct tggcgcttcg aagtagaatg cgggaaagga acctatgtcc
    16921 gcacattagc tgtagataca ggcaagaaac ttggttaccc tgcgcacatg tctgatctga
    16981 cccgaacagc aagtgccggt atgtcccaga aggatgctct gacattagaa gaagtagctc
    17041 aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc aatagaaaaa ggagtggaac
    17101 ggttcaaacg agtagatatt actgatgaag tatggcagaa agtaaaaaac ggtatgagac
    17161 ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga agaaattgct ttgttttata
    17221 aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga aaaaaacaaa ttaaaaccta
    17281 gtaaagtatt gagaaatgag gaataacgaa tgaatatcat caagatcaga catccttacc
    17341 aatcagaaca aattccagaa gacgatgtcg ttttagtgct gggatttttc gacggcgtgc
    17401 atatcggaca ccagaaagtc ataaagacag gtatggaaat tgcacaaaaa gaaggattga
    17461 aattagcatt gatgactttc aatcagcatc catctattgt ctttaaaaag atcgatccta
    17521 gcagcgtgaa atatttgact agccttaagc aaaaagaaga aaaaatggcc gctttgggta
    17581 tagattattt atatgaagtt gattttactt cttcttttgc tcatttagca ccgcaggaat
    17641 ttgttgatca atacatcgta gggttacatg caaaatatgc tgtttccggt tttgattata
    17701 cgtatggacc aaaagacatt gcagatgtcg ctcattttcc tgaatatgcc aaaggaagat
    17761 tcaaaatcgt cacagtttct aaagaagaag aacaaggtga aaaaatcagc tctacccgta
    17821 ttcgtggatt actagacagc ggacaagtaa aagaagcagc aaaactatta ggttgcacat
    17881 atgaggtaga aggggtagtt gtccatggcg atgcacgagg cagactacta ggattcccta
    17941 cagcaaatgt caaagtaaag agcacggttc atttgcctaa agaaggtgtt tatgtgtcgg
    18001 aaataaaaat tggggaaaca tggtatccag ccatgggttc aattggacat aatgatacat
    18061 tcggtgaagg acgggagctg actgtagagt tgtatatttt ggactttaat caagatatct
    18121 atggtgaaca tgtcgcggta cgctggaatc aatttttaag agatcaagtg aaattttcta
    18181 gtgtagagga attgattgaa caactcaagg aggatgaaca agcaacagct gaatattttt
    18241 ctcagcaata aaacattatg cataaaatct cttgtttatg tataactagc aattacaata
    18301 aaatgggtga taaataagcc caggaaaggg gctgtgacaa aagtcttgtc acagcctttt
    18361 tttccaaata aatggttttt cagaagtagc ttcttcggaa ataagccgaa atttcttgaa
    18421 gctgaccact tctgtcacaa cctctttctt tatagaaata aaaggacact agcaattcat
    18481 gaaaacgatt gctagagcgt atcatttgct ttagactata tatcttggtg tacactcact
    18541 ttaaggcgga aataaaagtt atccgtaata acaggtttag gtatgaaaaa ggagtgggta
    18601 ctatgtacaa ggaattagaa aacaaagtcg cagtagtaac tggtggctca aaggggattg
    18661 gaacagcgat tgctcaacga ttcgggaaag aaaaaatgaa tgtagttgtc aactataact
    18721 cggataaaga aggagcagaa aaagcagtca aagcaatcga agaggcaggc ggaaaagcag
    18781 tctctgtaca agcagatgtc agttctgaag aaggaataca gcgtttactt gatacggctt
    18841 tagaaaattt tgggacactg gacgtttggg ttaataatgc tgggatggaa aatcagcatc
    18901 caacccatga acttagcctt gaagattggg aaagagtgtt aaaagtcaat ttgactggcg
    18961 tttttatcgg aacgaaagcg gctttaaatt actttacaga gaataataaa aaaggaaata
    19021 tcatcaatat gtcttctgtt catgaacaga tcccatggcc aacgtttgct cattacgctg
    19081 cttcaaaagg cggggtaaaa ttatttaccg aaacggtagc aatggagtat gcaccgaagg
    19141 gaatcagaat caatagtata ggccctggtg ctatccgtac acctattaat gcggcaaaat
    19201 ttgatgatcc tgaacaaaaa gaactcttgg aaagtatgat cccactagga cgcgtaggtg
    19261 aaccaaaaga aatagcggcg acagctgcat ggttggcttc agacgaatcc agttatgtta
    19321 caggtattac actatttgca gatggtggga tgactttata tccttctttc caaggtggaa
    19381 aaggataagg gtgaataaaa atgggtttat tgattgcatt gatcccggca gttggctggg
    19441 ggatccagcc actgattttg aaaaagattg gtggcagacc aacaaatgag atattaggaa
    19501 ctggtattgg agcggtgctt gtcggcttac tcgttcaatt attcgcttct ccagctggta
    19561 tttcagtgac tacatttttg attagtttac tttctggtgc attttgggtt attggacaaa
    19621 caggacagta tacatctttc aacttgattg gtgtttctaa aacgatgcca atttcgactg
    19681 cattacagtt agtcggaacc tcgttgattg gtgtcttcgc ttttggagaa tggtctggaa
    19741 gtatggggaa aatcattgga gcagcagcaa tcattcttct tgtatttggt tctgctttaa
    19801 cggcaatcag tgatgatcct ggaaaaaaag gcggtatcac aaaaggaatt agtctattag
    19861 catcaacaag tattggttat tgggtataca gtgcattgcc taaactagtt gatgcaagcg
    19921 gactttccat tttctttccg caaatgatcg gtgtcttttt aggggcagtg atttatgtga
    19981 ttttccgtaa accaaccgca ttttctgaag acaagagttg gaaagcaacg atcgttggag
    20041 ccatctttag tgtgtcagca ttggcttata ttttttcagc aaatgaaaat ggggttgcta
    20101 ctgcatatat cattacacag ctaaacgtag ttatctctac attaggcggt atgtacatcc
    20161 tgcatgaaaa taagacacca aaagaaaaaa aattaacgat tatcggatta ttgctgatcg
    20221 tagtaggaag catggtcacg gtctttattt gaggataaag atggtaaaat ttcatttatt
    20281 aaaaaaatat ttgtttgaac caaacagtaa gtacttataa aaaaatagcg ctagaatttc
    20341 caacatctct ggaatttcta gcgcttttta attgtttaag atacaaggga aatatctgtc
    20401 tggatcaata atttttcatt gatccattga ctaaagttta cgacataatt gaagttacca
    20461 ccgattagta tctccgcatg attgtcttta taaagatcgt attgtaaaag acggtctttt
    20521 ttatttgcgt aaacatcgtt atggtactgg actaattgtt ctcggaattg attgattgct
    20581 gtaaaatcac gatattgaag gttgaaacgc atcgtgattg gtgcatttag catgaatcgc
    20641 tgcatttctt ttgctgcttc ctctgcttga tctccgtata ggaactcaaa aagattacgt
    20701 tcagaaaatt tccattgccc gccaaccttt gttcctttta gttttccttc atgaagataa
    20761 tttctgatag aacgtgtagt aacacctaat tgttcggcta attcatttac tgagtaaaaa
    20821 tctttcaatg ttcaccctcc tatgtgaatg gttctactga cttagttgat atttgcttaa
    20881 tcacgaaatg tagaaagaac gattttaatt aaggtcattg taaaaatcaa gtcagaaaat
    20941 tctaactttt atttctctaa agaaattcgt tctctcctaa agtgatatta tatttaaata
    21001 tacctgaaaa taatgggcag tattatgaaa gatcactgat attttctcgt attttttcct
    21061 tattcaaaag ttttgcagga aaattgctga aaaaataatt ttactatgta gaaaattcaa
    21121 agccattcaa tcagtgattt ttttcagcac aagaaagact ccttataaaa atagcagata
    21181 ctttgtcaat taagtgttta accagggatg ataaaaagga taggattgaa caaaacctgt
    21241 ataaggtgat gaatagtttc ctcgccagta aaaggtttat tcgtataaag ctttatcgcc
    21301 aaaatttatc atgacctttt ttatcttcaa tattaaatga aatcaattgt tcaatcaatt
    21361 cccagttgat tacacttttc caaggaactt tgatgacgtt atccgtatat acgtagcctg
    21421 cttcttggat atttttttca agggcacgaa tcgctgcttt ttccggtgcg atagaaaaat
    21481 gatttttagc aaaactaaat gcaataataa aggtaccatg gtcagtaaac ataggttggt
    21541 tccattttac agttgtgtct agctggggat acgtttctgt tatcttagca aaaaggtctt
    21601 tgatcttttg ttgatgttct ttttctttga tttgattgat gatcggctga aatttttcca
    21661 ttctaaaacg cctccattaa gtggtttgat ttattttacc agaaaaacaa tagttatgta
    21721 taaaaaacga acaaaacaca agtaatgttg taaccgctat atttatatga taagatgtct
    21781 caaataataa aatagaaaag agacataaaa atgattgact tggcaaagaa attcttacac
    21841 gaaaatatat attttgagtt gtatcatcat cgagccattt atacaaatga agatgctttg
    21901 gtaataaaga aagaacaagg atttacaggt acagagacca aaagcttatt cttaaaagat
    21961 aaaaaagata ataactacgt atatctgact tttactacga aacaaacgga tttcaaagca
    22021 ttagggaaga tcgtcggtaa aaggctttct attgtttcat cagaacgcat ggaagaacaa
    22081 acgggacaaa agcagggcgc tgtttctccg tttggttatg aaaaacagat tccgattatt
    22141 attgatgaag agctcttatc aaatgaaaaa cttgtttttg caccaggtag accagatcaa
    22201 acgatggtca tccaaacggt tgacttaaac aaaatcatcc agctacttga gatgccgata
    22261 tttttgatgc cggcaaacga aaaaaacaat taaccaagta aaatagtgca tacttggagg
    22321 tgaagcaaca tgataagtta tccattatta gaaagaaatg caacgattgg ggttactgca
    22381 ccatcctcag gcgtatcaga ggaacaccat tcgttactcg aatcagcttt aaagagaatg
    22441 gaagaaaaat ctttccaagt agttactgga aagacgcttt ggacgcaaga taaggaaaaa
    22501 tcaggtccag caagaaaacg agcagatgaa ttcaatcaaa tgatgcaaga tgaaaatatc
    22561 caaatgatct ttccgacttg gggaggagaa ttattgattg agattctcga ttttgtcgat
    22621 tttgatgctc tacaaccaaa atggatagta ggttattcag atattagtac gttgctactt
    22681 gctgtcacat taaaaacagg gatagccact gcacatggaa ctaaccttat tgatttaaga
    22741 ggagagtact tggatgaaac gacagcaatg tgggaaagtg tactgaaaac aagagaagga
    22801 gaatcgattc tacagcaatc ttctgaaaag tatcaaaaag tgtggcccca tgagcattca
    22861 tcatcttctg ttttccatct cactgaacca acaaattggc aatcagtcag cggagatgca
    22921 gtaaggatga agggacgatt attaggtggt tgtattgata cgatcaggca tttaatcggt
    22981 actccttatg gagatgtcca atcttttaaa agaaagtata atgatggaaa gccgatcttg
    23041 tggtttctag aaaactgtga gctatcgtca gttgatttaa gaagatcact tgttcagatg
    23101 aaactggcag gctggtttga tggcagctcg gggatcatgt tcggtagaag tgcggcagag
    23161 gatttgttag attataaaat gaaggatgta tacgaagatc tgtcaaagga attaaaacta
    23221 ccgatcttgt atgatattga ttgtggtcat gtaccgccac aactgacatt gattaatggt
    23281 gcctatgcag aagtagcggt agaagataga aaagcgactg ttttgcaaac gtttatttaa
    23341 aaaatttttt cctgccttgg agtctatcaa agaccaataa atctaaatag aaaagaaagt
    23401 gaaagaagct gacaaaatgc tgtcagcttc tttttgtata acatattaga aagtctaatt
    23461 atctataact gtcacatcga ttaactgttt cacgtcattg ggatttcctg taggaatagc
    23521 tactacagat gcattatcaa tgatttcttg agttgtttct ctaatgaacg ctggataaga
    23581 tgtttgatct gcaggcattt gccaatgtga aggaactgga taactagtga ttgtcccgtc
    23641 atggttactt gaatacacta aaactccctg gtaactgtat ttcccagtga gcataaccac
    23701 atctttcgga tagactgcac tccctgcatc ataaggggcg ataggtgttc ctgcaggata
    23761 atgaataaca tgtaattcga aatcacccga tgtatctcta aaagtattac ctctaatagc
    23821 tagccaaaca cgtgcgtact caatttgtag atcagaatat cctgctaacg gatctattga
    23881 ctgagaagac tgtgaggagg ttgtgtctat tgttgaagaa gaaatatttg tctcaactgt
    23941 actactagat gtagaagaag tattgggctc tgagacttta ctgcttgttg aagatgtatt
    24001 cgtttcagta gaagctgatt tctgagtact tgatttttca gctgaactgg tttgtctgat
    24061 tgaacttttg tctgtggtcg cagatggatc actattttct ttactaggag acgtacatcc
    24121 tacaaggaat aaagagacca acaataaaat aaaacctaac tttttcatat aaaatacctc
    24181 attatttttt taataccagt atagcataat cctgaatttt tgaatccgta aaaccttgtc
    24241 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataat
    24301 catcagtaaa attacttgtc acactattga taaggagtat ttaattgaag agaatgatta
    24361 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
    24421 cgttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
    24481 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
    24541 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
    24601 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
    24661 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
    24721 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
    24781 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
    24841 agaagcagtt ttgttttgcc atttctcatt ttggtttagt gtgctagtaa tataagcacc
    24901 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
    24961 ctttactatc tataacaagg acaacagttt taacctacag acattcatta aaattgggta
    25021 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagatag tcaacttaat
    25081 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
    25141 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat attaaaagaa
    25201 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
    25261 gaaaacaacc ctagagatat ataactaggt aaccagttta atgaaagaaa aaatcatcca
    25321 agaagtcgat tcttggattt tttagtggat ttgttcgcca aatacgttta gtatgcgtat
    25381 atgagaggtt gtaagcaaag cgttgtggtc tgagcactta aggaggaatc tgtgaaaata
    25441 gcttttcata tttttgcaga ttttgactta atgccgaagg tcagctttgc gattaccgtt
    25501 tatcgagagg ctgtaaaagc accgtgtagc tccgatgaca ataagcaagc atttttagag
    25561 ctaggtttcc ttgattctaa atatgcggct tatttcaatg agttggtgct tgaacgcgat
    25621 tttaagaagg tcgaaaacag gcacgacttt tcgacgtaag gagaaagaat ttcaatgata
    25681 gaaacaataa atcccgcaga aaacaaggtt tctgcgtaca ttcatatccc attttgcgag
    25741 catatctgct attactgtga ttttaataaa gtttttttag aagggcagcc agtagatgag
    25801 tatgtggaaa tgctgctgaa tgaaatgaaa ataatgctag aaagtcatcc gacagcgaat
    25861 ttagaaacac tttacgtagg gggcgggaca ccaacttctt tatctgctaa acaacttgat
    25921 cgactgttat caggtgctag agagatcctg ccttttcaaa atggtaatga gtttactgta
    25981 gaagcgaatc caggtgattt gacccgtgaa aaacttcaag taatgaaaaa ctatggcgtc
    26041 aatcgtttat ccatgggagt gcagacattt gataatcgct tattgaaaaa aattggacgt
    26101 aaacacaccg cagaagatgt gtatcaaact atgcaatttt tagaagcaga aaattttacc
    26161 aatgtaagca tcgatttgat ttatgcatta ccgggacaaa cactagaagg atatctggat
    26221 actttggaaa aagctttggc tttggatctg cctcactatt ctttgtactc attgatcctc
    26281 gaaaacaaaa caatgttcat gaactgggta cgacaaggaa gactcgaact gcctgatcag
    26341 gaaacggaaa ccagaatgtt tgaagagacg attgaagcaa tggaaaagca tggacgtcac
    26401 caatatgaaa taagtaactt tgggctctcc ggacatgaat ctcaacataa cctcatgtac
    26461 tggaacaatg accactattt tgggtttgga gctggtgcta gcggttatct tggagatatt
    26521 cgttaccgca acaaaggacc aatccaacac tatttacgcc cattacgagc aaatgaacgc
    26581 ccggtattgg aagaagaaaa attgacacga aaaaatcaaa tagaagaaga aatgttctta
    26641 ggactaagaa aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
    26701 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
    26761 gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa tgtgtttgag
    26821 cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat ttaaataaaa
    26881 aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat ttacatcaat
    26941 tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag
    27001 gagaagggct tcttgctata tttataagtg gattagcact tagaatatat gagtgctaat
    27061 gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg atcatccaac
    27121 actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag ggagtcaagg
    27181 ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga ttgctgttga
    27241 agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat tacgtggatc
    27301 atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt cagtctctag
    27361 gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata ttatctgagc
    27421 tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga ctgactggct
    27481 ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca gatatgggga
    27541 atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa ctagaaaaaa
    27601 tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg taccataaac
    27661 ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa gggatgtctc
    27721 agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc agtggacaaa
    27781 tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg atgttttctt
    27841 ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc aatattcata
    27901 tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg attcaagcaa
    27961 gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct actagtatgc
    28021 cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca gctaaacttg
    28081 ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga agctatttgc
    28141 agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa taatgaaagg
    28201 aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca gatgctcagc
    28261 cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa gctgaagcgg
    28321 aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat aaatacttgc
    28381 gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt gagcaattac
    28441 aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac aacctggaac
    28501 gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa
    28561 tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa atccctgcta
    28621 aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca gcgactgaag
    28681 atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta catgatcgcg
    28741 tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa acttacactt
    28801 tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact taggtacgac
    28861 taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg
    28921 aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag taggggaagt
    28981 tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac gccatatcgg
    29041 tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag aaatctcagc
    29101 aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa
    29161 agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc
    29221 tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg cagctgctct
    29281 tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg accttggtgg
    29341 cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg tattgtcaac
    29401 agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg actacatggt
    29461 agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg ctttacaacg
    29521 cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat
    29581 cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa tgacattgac
    29641 acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag taccagtacg
    29701 tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta ttttagtcgg
    29761 tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag gaaaagaacc
    29821 aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc aaggtggcgt
    29881 gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt cattaggtat
    29941 cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga ttccaacaag
    30001 taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca tccatgtatt
    30061 acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc aattgacaga
    30121 tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata ttgataaaaa
    30181 cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa aaatcacgat
    30241 caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag atgcagaagc
    30301 aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg atgtagatgc
    30361 attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg acgaagaaga
    30421 agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag caaacaacat
    30481 cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa
    30541 actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag ctcaaggcgg
    30601 agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata aataatcagt
    30661 tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt gaaaaatagt
    30721 ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg
    30781 atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg aagtcttagg
    30841 gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa
    30901 ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag aagtatcaga
    30961 agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat acgggcatgc
    31021 aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg gattcggcgg
    31081 cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct ttggaggagg
    31141 cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat atacggtcga
    31201 cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata atcgggaaga
    31261 aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa ctacttgtca
    31321 taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg gacgagtgat
    31381 gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag aaccttgtcc
    31441 aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga atgtaccagc
    31501 tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg
    31561 cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata tctttgatcg
    31621 tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag ctttggggga
    31681 tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg ctggaacaca
    31741 gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac
    31801 aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg aacaacaaag
    31861 agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga
    31921 cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt agtttttcta
    31981 gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatatg ttcgcctggt
    32041 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata tgataatcgt
    32101 catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga tattgcaagg
    32161 tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta tttttactga
    32221 agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt ttcaaaatac
    32281 gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc ccgaggtcta
    32341 aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat tcgcaaactt
    32401 gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc gacccaattc
    32461 caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta tcaagcaatg
    32521 atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt ctatgtacag
    32581 tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat aaaggattga
    32641 tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca taatatggag
    32701 aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga tatcatcaat
    32761 gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt ttgtgcagga
    32821 aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa atacacggtc
    32881 ccttcatggc gaccgtgcat ttttggtttt tattaaatat ttaatacttt attcaagaaa
    32941 tcttttgtcc gttcattttg cggattttca aagatttgtt gaggcgttcc atcttctaaa
    33001 aagtttccgt cgtcaatgaa catgacgcga ttggccactt ctttagcgaa ccccatttcg
    33061 tgcgtaacga taaccatcgt cataccttgt tctgctaact ttttcatgac gttcaagaca
    33121 tcaccgacca tttcaggatc gagtgcagaa gtaggctcat caaaaagcat gatgtcaggg
    33181 ttcatcgcta gagcacgagc aatagccaca cgctgttttt gtccaccaga aagcatttca
    33241 ggatacatat cttttttatc ttctagacca actgtctgca gtaatttgat tgcttttttc
    33301 tctgcttctt ctctagattc tttcttcagg tccatcggtg ctaaaatgat gttttccaaa
    33361 atagaaagat gagggaatag gttaaagtgt tggaatacca ttccgatatg ttgtctcacc
    33421 aagttgatat ctgtgctttt atccatcaag tgggcgccgt caataataat ttcaccgtca
    33481 gaaggttctt ctaaacgatt caagcaacgc aagaatgtac tttttcctga acctgaaggg
    33541 ccaatgatac agacgacatc tccttcgttg atggatacat tgatgtcgtt taatactgtg
    33601 ttatcgccgt attttttgac taaatgctca actaagattt tttctgccat cttacttcac
    33661 cttcttttct aaaacttttg ctaattttgt caatgctgtg atcaaaatca agtacatgat
    33721 cgcaatgatc aagtaaacat acgtactttg tgttgtacgt gcaacaatga tcttaccagt
    33781 ttgcagcaat tcgactacac cgattgcgga aatgatcgtt gtgtctttta atgagataac
    33841 gaactgattg acgaatgatg ggatcatgat gcgtatcgct tgcggcaaaa tgattttttg
    33901 cattgtccga ttataagaaa gacctaaact tctggaagct tccatttgtc cgacaggaac
    33961 ggcgttgatc ccgcctcgaa caatctctgc gatataagca cttgcgttaa gagtcaaggt
    34021 gataacacca gcaaggaagt caggaatcgt aaatccaacg ataccaggca acccaaagaa
    34081 aataaagaac gcaagaacca tcattggaat tcctcggatg atatcgacat aaatactagc
    34141 aattgtgcga agagcgcgta ttggtgctac actgaataat ccaaaaatca caccgattac
    34201 tatagccaaa gcaaaggaga tcaaagttag gagaattgtt ctccaaagtc ctttcaacaa
    34261 tgcttgatag ttgttttgga taagtccaag aaatgttgat tcatcagcag tggaagactc
    34321 actggaagtt tctgaaccat ccgctacata ttctttaata atcttatcgt attcgcctgt
    34381 tctttttaat tctttcaatc cttcattgaa catattaaga agctcaggat tttgtccttt
    34441 tttaacggca aatgcatatt ttccgccaga ttcttttttg atcggtgttt ccaattcttg
    34501 tccttgtttg atcgcataac cgattaccgg ataatcatcc atcattgcat cgattgcgcc
    34561 aacttttaac ccgtcgtaca attgatcagc agcatcaaaa tatttgatag aatagccata
    34621 ttcagattca tggtcgctta aaaattcagc actttcggta ccaacttttg ctccgactgt
    34681 ttttcctttc aaatcttcat aagacttgat cgaagtatct ccttttttga cagctaattg
    34741 gatcccgcta tcaaagtaag aatcagaaaa atcatagctt ttctttcgtt catctgttac
    34801 agtcattccg gcaatcattc cgtctgcttg tcctgattcc aaagcttgca aagcagcaga
    34861 gaaaccacgg tagtccattc ggatgttaaa tccttgcagt ttggcgatgt tttccatcaa
    34921 ttttacatcg atgccttcgt actcgccctt tgcgttttga aattcaaatg gagcaaaagc
    34981 agagtcactg gcaattacgt atacgtcttt tttgggctct attttttgca ttgtctcgct
    35041 tgtgcttgac tctgccgttg tagtgttgtc agcttgtgca acatgaccaa acgcaaatgt
    35101 cacaagtcct gtcattaaga aaacaaatga gtgaaataaa tgctttcttt tcataatcaa
    35161 cctcctaaat ttccaatatt ttattattat aggcgaaaag tgctgaatca gacaagactg
    35221 taagctcgaa aaaatgaaaa aaatgcgagt ttctaacatt ttatgttata aaacataaca
    35281 tgtataataa gataaaaaag tggcgaaagt atgttcggtg tggtataatt ttttagtaag
    35341 gaatgccttt gctttccttt tctttttatt ggtcgtaaaa tgaaaaggaa tgaatgaaaa
    35401 ggaggacgaa gaatgattga gagagatacc tcccgacact ttgagcttgt ctcgaaatac
    35461 caaccggctg gtgatcagcc tgaagcgatt aaccaattag tggatggcgt agtcggcggg
    35521 aaaaaagcac aaatcttatt aggtgcgact ggaacaggga aaacatatac gatttccaat
    35581 cttattgaga aagtgaataa accaacactg attattgccc ataacaaaac attggctggt
    35641 cagctgtacg gggaattcaa ggaatttttc cctaacaatg ctgttgaata ttttgtttct
    35701 tattatgatt attatcagcc tgaagcttat gtgccttcaa gcgatacgta tatcgaaaaa
    35761 gattcaagtg taaatgatga gatcgacaaa ttacgacatt cggctactag ttcattatta
    35821 gaaagaaatg atgtgatcgt cattgcatcc gtttcatgta tttttggatt aggctcgcct
    35881 tttgagtacc aaaaacaagt agtttcgatt cgacagggag cagaactaga tcgcaatcag
    35941 ttgattcgtg acttagtcag tatccaattc gaaagaaatg atatagattt tcagcgcggt
    36001 cgttttcgag tgcgcggtga tgtcgtggag atttttccgg cttcaagaga tgaacgtgcc
    36061 ttacgtgtgg agttttttgg agatgaggtc gaacgtatcc gagaagtcaa tgcactgaca
    36121 ggtgaagttt taggagaaac ggaacacgta gcaatcttcc cggcgactca ctttgtgacg
    36181 aatgatgaac atatggagca tgccgtagcg aatatcaaag cggaattaga acagcgttta
    36241 actgtcttgc gaaatgaaaa caaacttctg gaagctcaac gtttagaaca gcgaaccaat
    36301 tacgatattg aaatgatgtt agaaatgggc tacacgtcgg gaattgaaaa ttattctcgg
    36361 catatggacg gacgtaaaga aggtgaaccg ccttatacgc tattggattt ctttcctgaa
    36421 gattttttga tcgtggcgga cgaatcgcac gtaaccatgc ctcaaatcag aggaatgtat
    36481 aatggtgacc gagcgagaaa acagatgcta gtagattacg gtttccgttt gccatctgct
    36541 ttagacaatc ggccacttcg gttagaagag tttgaaaaac atgtcaatca gatcatttat
    36601 gtctctgcga cgccaggacc atatgaacat gaacaaacag ataccgttat ccaacaaatc
    36661 atccgtccga cagggctgct tgacccagtg atcgaagttc gtccaatcat gggacaaatc
    36721 gatgatctag taggcgaaat aaatgaacga gtcgaaaaag accagcgggt gtttgtaacg
    36781 acattgacga aaaaaatggc agaagacctc acagattatt tcaaagaatt agggatcaaa
    36841 gtcaaatatt tgcatagtga tatcaaaacg ctggaacgaa cagaaatcat tcgagatctt
    36901 cgattaggtg aatttgatgt gttagttgga atcaatttgc ttcgtgaagg attagacgta
    36961 cctgaagttt cccttgttgc tatcttggat gcggataaag aaggcttttt acgtagtgaa
    37021 cgttccttag tccaaacgat tggacgagct gctcggaatg aagaaggtaa agtaatcatg
    37081 tacgcagaca aagtaacaga ttctatgcgt ctagcgatgg atgaaacgtc aagacgtcgt
    37141 acgattcagc agaagtacaa tgaagaacat gggatcgttc caaaaacgat cattaaagaa
    37201 atccgagatt tgatttctat tacgaaagaa tcggaagatg atacaaaaga agcggtacaa
    37261 gtctcgtacg aagaaatgac gaaggaagaa aaagacacgt tattgatgaa attagaaaaa
    37321 gaaatgaaag atgcggcaaa agctttggat tttgaaacag ccgcgaatgt gcgagatatg
    37381 attttagaat taaaagcttc aaagtaggag gacaacatgg ctaacgataa aattacgatt
    37441 catggtgcac gtgcccataa cttgaaaaat atagatgtaa cgattccacg agataaattc
    37501 gtggtcgtta caggtttgtc cggttctgga aaaagttccc tagcatttga tacgctgtac
    37561 gctgaagggc agcggagata cgtagagagc ctttctgctt atgctcgtca gtttttagga
    37621 caaatggata agccggacgt agatagcatc gacggattga gtccggcgat ttctattgat
    37681 cagaaaacaa caagtaaaaa tcctcggtcg acagtaggta ctgtgacgga aatcaatgac
    37741 tatttacgct tgttgtttgc acgtgtagga catccgattt gtcccaacga tcatatcgag
    37801 attaccagtc aatcagtaga tcaaatggca gatcaagtaa tggctttgcc tgagcgcaca
    37861 aagatccaag tgttggcacc gatcgtggta aagaaaaaag gacaacataa aaaaatattc
    37921 gaacgaatcc aaaaagaggg atacgtccgc gttcgagtcg atggtgagat ctatgattta
    37981 agtgaagtgc ctgagctgga gaaaaacaaa aaacatgata tcgcgatcgt catcgaccgg
    38041 atcattgtga aagaagggat tcgttctcgc ctatttgatt ctttagaggc ggccctgcga
    38101 ttagcagacg gctatgctct ggttgacgtg atcggtgaag aagaaatgtt gtttagcgaa
    38161 cactatgcct gtccttattg cggatttact gttggcgaac tagaaccaag actgttctca
    38221 ttcaatgcgc cttttggagc ttgtccagac tgtgatggtt taggtgtcaa attagaagtc
    38281 gatcaagatt tagtgattcc agatagaacg aagacactta gtcaaggtgc tatcgttcca
    38341 tggaatccaa tcagctctca gtattatcct caaatgttag cgcaggcttg tgaaagtttc
    38401 ggtatcgatt tagatacacc attcgaagaa ttaccggaag agcatcagga gatcatcttg
    38461 aatgggacag atgatctttt ccatttccat tatgaaaatg attttggtgg cgttcgcgat
    38521 gtagaaacga ctttcgaagg ggtattgaag aatatcgagc gtcgttatca tgatacgaac
    38581 agtgatttca cgcgtgagca aatgcgtctt tatatgacag aattaacttg tcagacatgt
    38641 aaaggttatc gattgaatcc ccaagctcta tctgttcaaa tcaatggaac gaatatcggg
    38701 caaacaaatg agttgtctat tcaaaaagca gtagatttct tctcaaactt aacgttatct
    38761 gaacaagaga aagtaattgc caaaccgatt ttaaaagaag taaatgacag actttcattc
    38821 ttggaaaatg tgggactaga ctatctaact ttgagtcgcg cttccggaac attatctggg
    38881 ggagaagcgc agcgaatcag attggctaca caaattggat cgaatctttc tggagttttg
    38941 tatattttag atgaaccatc gatcggattg catcaacgcg acaacgatcg actgattggc
    39001 tcattaaaga aaatgcgtga tcttggcaac accctcgttg tagtcgaaca tgatgaagat
    39061 acgatgtggg cagcagatta tttgattgac gtcggacctg gggccggaga acaaggcgga
    39121 caaatcgtgg ctgcaggcac tcctgaagaa gtagctaatg atgaaaattc tttgacaggg
    39181 aaatatcttt cagggaaaaa atcaatccct gtaccaaaag aacgccgtaa aggtaatggg
    39241 aaagcaatca agatcactgg tgcttcagaa aacaatctga aaaatattag tgttgaattt
    39301 ccattaggag aatttgtagc agtcacaggt gtgtctggtt ctggaaagag tacattggtc
    39361 aatagtatct taaagaaatc gttagcgcaa aaattaaata agaattctgc taagccaggt
    39421 aaattcaaga caatttccgg ctacgaaagt atcgaaaaga tcatcgatat cgatcaaagc
    39481 ccaatcggcc ggacgccgag aagtaatcca gcgacttata caagtgtatt tgatgatatc
    39541 cgtgggttat ttgctcaaac gaacgaggca aaaatgcggg gttataagaa agggcgtttt
    39601 agtttcaacg taaaaggcgg tcgttgtgaa gcttgtcgcg gggatggaat tattaagatc
    39661 gaaatgcact ttttgcctga tgtctatgtt ccttgtgaag tatgtcatgg taaacgatat
    39721 aattctgaaa cattagaagt gcattacaaa ggaaaaagca ttgctgatat tttggaaatg
    39781 acagtagagg atgctgtaga attcttcaag cacattccaa agattcatcg caaactgcaa
    39841 acgattgttg atgttggctt aggttatgtg acgatggggc aaccagcaac gacattgtcc
    39901 ggtggtgagg cacaacggat gaaacttgcc agtgaattgc acaaaatctc taatggaaag
    39961 aatttctata tactagatga accaacgaca ggacttcata gcgatgacat cgcccgcttg
    40021 ttgcatgtat tacaaagatt agtagatgct ggtaacacag ttttagtgat tgaacacaat
    40081 ctagatgtaa tcaaaacagc agattatatc attgatttag gaccagaagg tggagaaggt
    40141 ggaggaacga tccttgcgac tggaacacca gaagaaatca ttaacgtaaa agaaagttat
    40201 acaggtcact atttgaaaaa aataatggta taaagaggat agatagctga gatagttaaa
    40261 aaaacataaa aaaatgaatt tttgcttgtt ttattaatat cagtgttcta gaatcattac
    40321 atggtagttc tttctacact gattggtata gataaaaact tcctataagt tcaatccgtt
    40381 caaaaaagaa ttgagcaatc ggaggacttt cctttgattg cttttttctt tatggtatga
    40441 tattccttgt gtagttggaa aagaaaaaca agagattatg agtaagtgag agataatgat
    40501 gaacagtatt gaaaatgaag aatttatccc tatattatta ggaagcgaca tgaacgttta
    40561 tggtatggca cgttctttta atgaagcata cggcaaaatc tgccaagcat atgcttcgga
    40621 tcagttagcc cctacccgct acagtaaaat tgtcaatgta gaagttattc caggatttga
    40681 taaagaccct gtatttatcg agacgatgct tcgtttggca aaagaaagat attctgataa
    40741 gagtaaaaaa tatcttttga ttgcttgtgg tgatggatat gcagaattga tctcccaaca
    40801 taaacaagag ctgtcagaat attttatctg tccatatatt gattattctt tatttgaaag
    40861 gctgatcaat aaagtaagct tttatgaagt ttgtgaagaa tatgatcttc cgtatcctaa
    40921 aacattgatc gtcagagaag aaatgttagt aaatggacat ttggaacagg aattgccttt
    40981 tgaattccca gttgctttga aacctgcaaa cagtgtggag tatctttctg tccaatttga
    41041 aggaagaaaa aaagctttta ttttagagac tagagaagag ttcgatttaa ttttaggccg
    41101 gatatacgaa gccggttata aaagtgaaat gattgtacaa gattttatcc cgggtgatga
    41161 tagtaatatg cgtgtgctaa atgcgtatgt agatgaagat catcaagttc ggatgatgtg
    41221 ccttggtcat ccactattag aagatcctac accagcatcg attggtaatt acgttgtgat
    41281 catgccagat tataacgaaa aaatctatca aaccattaag gcatttttgg aaaaaatcga
    41341 atatacaggc tttgccaatt tcgatatgaa gtatgatcct agagatggag aatataaatt
    41401 atttgaaatc aatttgagac aaggtcgtag cagtttcttt gtgacattga acggcttgaa
    41461 tctggctcgt tttgtcacag aagatcgagt gttcaacaaa ccctttgttg aaacgaccta
    41521 tggaacgaat caatcagata aagcaagatt atggatgggt gtgcccaaaa aaatcttttt
    41581 agaatatgct cgggaaaatg aagacaaaaa actagctgag caaatgatca aagaaaatcg
    41641 ttacggtact actgtatttt atgaaaaaga ccgatcaatc aagcgctggt tgttgatgaa
    41701 atatatgttc cataactata ttccaagatt caaaaagtat tttcatgtga aagaaggcta
    41761 gttcatggag aattttttca gtattttagg cggaatgggc acgatggcga cagaaagttt
    41821 tgttcgcttg atcaaccacc gaacgaaagc tacaaaagat caagaatatt tgaattatgt
    41881 cttattcaat catgcaacag ttcctgatcg tacagcttat attttagatc gatcagaaga
    41941 aaatccaatg ccgtttctgc tggatgatat tgagaaacaa aatttattgc gaccaaactt
    42001 cattgtgttg acttgtaaca ccgcacacta cttttttgaa gagttacaag cggcaacgga
    42061 tattcctatc ttgcatatgc caagagaagc agcaaatgaa cttgtccgtc agcatacgac
    42121 agggagagta gcgattctag gaacagaagg aagtatgaaa gctggaatct atgaacgcga
    42181 agtcaaaaat cttggtttcg aaacagtgat tcctgatact gcgcttcaag aaaaaatcaa
    42241 ttacctgatc tatcatgaga tcaaggaatc ggattatttg aatcaagagc tctattacga
    42301 aatcttagag gaagcggtag agcgcctaaa ctgtgaaaaa gtcatcctag gttgcacaga
    42361 attatctttg atgcatgaat ttgctgaaga taatcattac ccagtgatcg atgctcaatc
    42421 tattttagcg gatcgaacga tcgaacgtgc attagctgaa agaagcgagg cattggatac
    42481 agcatcggaa aagtaaaaaa attggatttg gggcaaatat cctaaaagat atttggtcca
    42541 aatctttttg taaaagaaaa agaaaactgg taatgatacg cccgggatga tataatttta
    42601 ttatgcacaa tgtgagaggg gttcgtaaat atgacagata atttgcaatt agtcatcata
    42661 acaggtatga gtggtgcagg gaaaacagta gctatccaaa gttttgaaga tatgggctat
    42721 ttttgtatcg ataatatgcc gcctcgattg attccaaaat tctgggaatt gatcaaagaa
    42781 tcaggtaaag tcacaaaaat ggctttagga gtggatttgc gttctcgttc cttctttgaa
    42841 gaaatccaaa acatgctgat cgaaatcgaa aatacgaact tcatcgacac ttctatctta
    42901 tttttagatg cttcagacga agaactagtg gctcgctaca aagaaacaag acgagcacat
    42961 ccaatggcga tggacggttt gatcactgaa gggatacgta aagaacgggc gatactagac
    43021 gacttgaaag cccaagcttc cgtgatcatt gatacgacaa cattgacacc acgacaatta
    43081 cgtgaaaaaa tcaatgagtc attcaaatca aagggagacc aaggatttcg gatcgaattt
    43141 gtatcatttg gttttaaata cgggttgccg atcgattcgg atattgtgat ggatgtgcgt
    43201 tttttgccca accctcatta tatcccggaa ctaagaccat tgaccggaaa agatcagcca
    43261 gtctatgatt atgtgatgtc ctttgatgaa acgcaatcat tctaccataa atttttggat
    43321 cttctggaaa ctgtattgcc tggatacatt aaagaaggaa aaagcagctt gacagttgca
    43381 atcggctgta caggcggtca acaccgttct gtggcattga ctgaacgttt ggcaaatgct
    43441 ttagggaaaa actataaagt caatgtgaca catcgggaca aggataaaag gaaagagacg
    43501 gtgaaccggt cgtgaaaatg aaaacctacc ggataagaaa accaaagatc gtagtggtcg
    43561 gcggtggcac tggtctgcca gttatcctga aaagtttacg aaatcaagga gcagatatca
    43621 cagcagtagt gactgttgca gatgatggtg gaagcagcgg tgcaatcaga gaatcgatcg
    43681 ctatggctcc tcctggtgac ttgagaaatg tcttagtggc gttatcggat atgccacaat
    43741 tctatgaaga tattttccaa taccgtttca aaaaggaaga tcagttttta gccaatcata
    43801 cgatcggtaa tctgatcatt gccgcagttt ccgagatgcg gggaagcact tatgaggcga
    43861 ttcaattatt agcaaaaatg atgcatgtag atggacatgt ttatccttct tcagaaactc
    43921 ctttgacctt gcatgctgtt tttaaagacg gaacgactgc agtaggagaa tctaaaatcg
    43981 cgatcaaccg taaaaccatt gaccgtgtgt atgtctgcaa cacaaatgat aaaagtgaag
    44041 ccaaagcggc tcgaaaagtc gtttcagcaa ttatggacgc cgacatggtc gttttgggac
    44101 ccggaagttt attcaccagt atccttccaa atttagtgat accagaaatc ggcgaagcaa
    44161 tgctcaagac aactgctgaa accgtctata tctgtaatat catgacccaa aaaggggaaa
    44221 cggaacattt cacagatgcc gatcatatac gtgttttaca caagcatttg aatcagcctt
    44281 ttatcgatac agtattggtc aatacggaaa aagtgcctga agattatatg gacccagaga
    44341 tctatgatga gtatctagta caagtcgcac acgacttcaa cggattgaga aatgaaggat
    44401 gtcgagtgat ttccaccgac tttttagaat tgaaagatgg tggtgtcttc cacgatggag
    44461 acaaagtagt agaagaattg ttccgtttag ttttcggttc aaaatattaa gcaatttatt
    44521 gttggagaaa ggaggagaat aggatgtcat ttgctgcaga tgtgaagaaa gaactaactg
    44581 gtttggaagt tcatcgagaa catgcgaaag cggaattagc tgctttgctg cgaatgaacg
    44641 gctctttagg tattgtgaac cagcagttta ttttgaacgt ccaaacagaa aatgctgcaa
    44701 ttgccagacg tatctattct cttttgaaag atcattatca ggtacgaagt gagctactcg
    44761 ttcgtaaaaa aatgaaatta aaaaagaaca atgtctatat cgtccggttg aagcaaggca
    44821 caaaagatgt gttgatggat cttgatatca tggatggtat gatgttcaat ggacacgttt
    44881 cggacgagat tatgggaaat tcgcaaaaaa tgcgttctta cttacgtggt gcttttatgg
    44941 cctcaggttc agtcaataat ccggaaacga gtcgttatca tttggaaata ttttctattt
    45001 atgaacagca taaccaagac atttgcgata tgttaaatta ttacggtcta catgctcgaa
    45061 cgcttgaacg gcgcaatggc tatatttcgt atttaaaagg agcagaaaaa attgctgatt
    45121 tcttaacatt gattggtgca accaactcaa tgctccgatt tgaagatgtt cgaatcgtac
    45181 gggatatgag aaattcggtc aatcgtctag tcaattgtga gacagcaaat ctgaacaaga
    45241 cgattgatgc ggcttctaaa caaatcgaaa acatcgaatt gatcgaagca aaggtcggtc
    45301 tacatgccct tcctgaaaaa cttcaggaaa tcgcagaact tcggcttcaa catccagagg
    45361 tcagcttgaa agaattaggg gaaatgattc cctcaggtgc aatatcaaaa tcgggaatta
    45421 atcaccgaat tcgcaaaata aatgaatttg cggaaaaact gcgtgaagaa gcagtttaat
    45481 ccaaagaaaa aactgataca tgaagcagga gatgcgaatg tatcagtttt ttttaaaagc
    45541 tatacttgct tttcttccgt caaactataa ttccaacttt actggagtaa cataatgttg
    45601 aaaggcttct attgtttttt aatacaatcg tataaaaagc ggggagacaa ctgaatgaag
    45661 attttagttt tatacataaa cttttgctta atatagaagt cctgatatag ataggatcaa
    45721 gtatttatct ataactggag attaattaaa aactggaagg aactgctcga tcaaccttga
    45781 gaactgtttt tcttacttct ggtcaagatt ctcgtggttg gtggataagg atggttgata
    45841 agttcattgg attagctagt cgaaaacaac caggtgtaat taatgtagaa gttttaacaa
    45901 ggggatggag tgatttattc agtgataatc ataagttcat aaatagtaat tgggatgtat
    45961 ggttggtata cgttatacct gaacttgaac tatcaagatt tggtttagaa tttgagaatc
    46021 gatgtttata aagggagggc aaaaaatgtg cttttttaca acttttacta tcattatgat
    46081 tgcagtactt tttatttttc tagatatagc aaagcgaaat acagcatttt tattatatcg
    46141 ggttttactt cgagcaggtc tcataacatt tatatcgatc gttggatttt ttctattcac
    46201 agttattgta tttatttgga gaactacagc accaccattg ccagagatta catatggaga
    46261 atttccgttt agtcttgaat atgaaataga tgaggaaaga attttgattg aagatacggt
    46321 gattgcagaa tttataggaa gttttagagg gaatgtgaca actcaaccta gacgaagttg
    46381 gagtataaaa cttttaaata atagtggaga gttgaaagca tttcattatg aattcttaat
    46441 aatagaaagt gaagatgtaa caatcacttt aagtagtggc tctgctggat attttatgaa
    46501 tgatggtgga agcaagccac agctcacagt ccgtgatcgt attgcaggtt ttatgcattt
    46561 ttctcactat gacttagaag aagctcgtaa aatacttgca gaatatggga tcacattaat
    46621 caattgggaa tatgcgccac cgataagaaa ttttttcaat gattattatt agtcttataa
    46681 aaaacagaat gtaaaattta tgtgcgcgag tatttagttt tccatttttt gtttgataga
    46741 tatatagcat tgttgaagca tgggatagtg gtgccaattt tatcaatgag gttggtcagg
    46801 cagttaacat aattggtgat gctataaatc catttaattg gaattggagg aattaggtgt
    46861 ggaaacagtg agattatgga tattcatgaa aaattttcgt tcggctttga gatatgttat
    46921 tctggaatca aaatcaggtt attatctgat tgacacagat cactatactt tttggggagg
    46981 ctaccttttt ccgattttta attggataat cccccataag gttatgaaaa ttaacttgcc
    47041 tgcagaagaa ataaatagat taatactcaa caaagattta caaaggaaaa gagagaaaga
    47101 acattctaat gttgcttgga taggtccagc atttgcagta gtgacttctt caattgtcgg
    47161 tcctatgcta gagagtattt atagtgattt ttctattgga atacgttatt tatttgttac
    47221 tttaagtata gctgttcttt tgtgcataaa gggttatgtt agcaagaaga ataaagatat
    47281 tttaacacta atcggtgtaa acaatttatc tagtactaaa attatactgt ttcctgaatc
    47341 tataaaagaa ggatcaaggt tgttgataat ttttataatg cttatactgt tttctgtttt
    47401 atttggggtg atgtttata
//
LOCUS       DACDRK010000012        45099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDRK010000012 DACDRK010000000
VERSION     DACDRK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.012"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            770..1102
                     /locus_tag="IWJ77_000792"
     CDS             770..1102
                     /locus_tag="IWJ77_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9452434.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            1693..2022
                     /locus_tag="IWJ77_000793"
     CDS             1693..2022
                     /locus_tag="IWJ77_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452435.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            2009..2350
                     /locus_tag="IWJ77_000794"
     CDS             2009..2350
                     /locus_tag="IWJ77_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9452436.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            2464..2703
                     /locus_tag="IWJ77_000795"
     CDS             2464..2703
                     /locus_tag="IWJ77_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452437.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2770..3009
                     /locus_tag="IWJ77_000796"
     CDS             2770..3009
                     /locus_tag="IWJ77_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452438.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            3095..3766
                     /locus_tag="IWJ77_000797"
     CDS             3095..3766
                     /locus_tag="IWJ77_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9452439.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3819..5795
                     /locus_tag="IWJ77_000798"
     CDS             3819..5795
                     /locus_tag="IWJ77_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9452440.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            5808..6464
                     /locus_tag="IWJ77_000799"
     CDS             5808..6464
                     /locus_tag="IWJ77_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9452441.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            6560..7387
                     /locus_tag="IWJ77_000800"
     CDS             6560..7387
                     /locus_tag="IWJ77_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452442.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            7397..7645
                     /locus_tag="IWJ77_000801"
     CDS             7397..7645
                     /locus_tag="IWJ77_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452443.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            7676..9787
                     /locus_tag="IWJ77_000802"
     CDS             7676..9787
                     /locus_tag="IWJ77_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9452444.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(9825..11882)
                     /locus_tag="IWJ77_000803"
     CDS             complement(9825..11882)
                     /locus_tag="IWJ77_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9452445.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(11996..12208)
                     /locus_tag="IWJ77_000804"
     CDS             complement(11996..12208)
                     /locus_tag="IWJ77_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452446.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            12395..12862
                     /locus_tag="IWJ77_000805"
     CDS             12395..12862
                     /locus_tag="IWJ77_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452447.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            12859..13101
                     /locus_tag="IWJ77_000806"
     CDS             12859..13101
                     /locus_tag="IWJ77_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452448.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            13119..13421
                     /locus_tag="IWJ77_000807"
     CDS             13119..13421
                     /locus_tag="IWJ77_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452449.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            13492..15546
                     /locus_tag="IWJ77_000808"
     CDS             13492..15546
                     /locus_tag="IWJ77_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452450.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            15546..17378
                     /locus_tag="IWJ77_000809"
     CDS             15546..17378
                     /locus_tag="IWJ77_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9452451.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            18073..19989
                     /gene="ltrA"
                     /locus_tag="IWJ77_000810"
     CDS             18073..19989
                     /gene="ltrA"
                     /locus_tag="IWJ77_000810"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9452452.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            20106..20906
                     /locus_tag="IWJ77_000811"
                     /pseudo
     CDS             20106..20906
                     /locus_tag="IWJ77_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            20903..23434
                     /locus_tag="IWJ77_000812"
     CDS             20903..23434
                     /locus_tag="IWJ77_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452453.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            23484..23816
                     /locus_tag="IWJ77_000813"
     CDS             23484..23816
                     /locus_tag="IWJ77_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452454.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            23817..24410
                     /locus_tag="IWJ77_000814"
     CDS             23817..24410
                     /locus_tag="IWJ77_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452455.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            24573..26267
                     /locus_tag="IWJ77_000815"
     CDS             24573..26267
                     /locus_tag="IWJ77_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9452456.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            26955..28832
                     /gene="ltrA"
                     /locus_tag="IWJ77_000816"
     CDS             26955..28832
                     /gene="ltrA"
                     /locus_tag="IWJ77_000816"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9452457.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            28963..29340
                     /locus_tag="IWJ77_000817"
                     /pseudo
     CDS             28963..29340
                     /locus_tag="IWJ77_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            29340..30545
                     /locus_tag="IWJ77_000818"
     CDS             29340..30545
                     /locus_tag="IWJ77_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9452458.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            30561..31205
                     /locus_tag="IWJ77_000819"
     CDS             30561..31205
                     /locus_tag="IWJ77_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452459.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            31216..32022
                     /locus_tag="IWJ77_000820"
     CDS             31216..32022
                     /locus_tag="IWJ77_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452460.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            32044..32274
                     /locus_tag="IWJ77_000821"
     CDS             32044..32274
                     /locus_tag="IWJ77_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452461.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            32820..33095
                     /locus_tag="IWJ77_000822"
     CDS             32820..33095
                     /locus_tag="IWJ77_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452462.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            33137..33559
                     /locus_tag="IWJ77_000823"
     CDS             33137..33559
                     /locus_tag="IWJ77_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452463.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            33578..35170
                     /locus_tag="IWJ77_000824"
     CDS             33578..35170
                     /locus_tag="IWJ77_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452464.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            35182..35517
                     /locus_tag="IWJ77_000825"
     CDS             35182..35517
                     /locus_tag="IWJ77_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452465.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            35664..35858
                     /locus_tag="IWJ77_000826"
     CDS             35664..35858
                     /locus_tag="IWJ77_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452466.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            35890..38262
                     /locus_tag="IWJ77_000827"
     CDS             35890..38262
                     /locus_tag="IWJ77_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452467.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            38323..39783
                     /locus_tag="IWJ77_000828"
     CDS             38323..39783
                     /locus_tag="IWJ77_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9452468.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            39794..41998
                     /gene="topB"
                     /locus_tag="IWJ77_000829"
     CDS             39794..41998
                     /gene="topB"
                     /locus_tag="IWJ77_000829"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9452469.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            42244..42837
                     /locus_tag="IWJ77_000830"
     CDS             42244..42837
                     /locus_tag="IWJ77_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9452470.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            42853..43752
                     /locus_tag="IWJ77_000831"
     CDS             42853..43752
                     /locus_tag="IWJ77_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9452471.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            43755..44240
                     /gene="ssb"
                     /locus_tag="IWJ77_000832"
     CDS             43755..44240
                     /gene="ssb"
                     /locus_tag="IWJ77_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9452472.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            44606..44866
                     /locus_tag="IWJ77_000833"
     CDS             44606..44866
                     /locus_tag="IWJ77_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452473.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT        16600 a         7129 c         9226 g        12144 t
ORIGIN      
        1 tattacaaat attacaaata aatagttagt attccggagt ttttagatta aaagtttatg
       61 aaaacaatca caatcgtagg ttttaaattc ataatcgtta gatattttag ttatttttca
      121 ctcaaaaaaa tagggaacta gtgtttgcat atgtgctttt ttgagttata ataattcatg
      181 aagagaggta attcacttat ttccccaagc tgaaaagcct gatattactt cttttcaggc
      241 agtgctgcta atattcaatt acactttttt gtatattttt agttgtttta aatttaattg
      301 tctgctatgc ttgtgatgaa ttgattttcg ttctgcactt ttggacgata aatttggaca
      361 attgaaaata caaaaaaaag aaaggccctg tgagactcgg aaggctggta accttcgtcg
      421 acgtccacag gtggcattaa gatcactccc ttaatgcgtt tacctttcag tgttacggta
      481 ttacccgtaa cggatttagt ataacagata ttcaaaagtg ataccacttt ttttggctgt
      541 gttaaggtta aaaatacgtg atattacgat gaatctatgc ctaagcataa tgttataaaa
      601 atgtccgtgt ggctctttgc cgtatgacga acgctgaaat tagagggaac gcatgtttgg
      661 atgcgttccc tcttttttgt ttgtcatgaa cgaaaatcaa ttctctattt ttgaaaaata
      721 acaagaaaaa cttgttaaaa atatttaaag taagtgggtg gattatttta tgtcatatac
      781 aggaattttg agtctcgaag atatttgtca ctacggtaaa cggtgtaccg ctacagaaaa
      841 gatcacgaag aaactatcaa ctggtcaaag taaaaccgtt gtgcagtgta agaaatatat
      901 aatccagaaa gacaaagtct cagaagaaat gatttattac attgggaaac aaaaacaaat
      961 aattttaaaa gatcctatcc ctttaaaaga gctatatcca acaatcaaac atgtttatga
     1021 ccaaaacggt gtactgattg gtcgaagaaa aaatggagtc ttgcgttgta ccgcaaaagg
     1081 gatggggcgt ttaatcagct gaattgatca ttctcataac ttgtatttta tcgtttttcg
     1141 cttgcttgaa aatattgtcg agacttctaa aggaacttag aatcactaga aaaataagga
     1201 gtagacttga tctatcaaca attcctttcg gggggtgacg gggtaggttt ttacgattgg
     1261 taacgtaaaa aattggtcaa tcgagtaaat gcccttgttt ggatagtggt gaaatgaatt
     1321 tgtgtaaata atatgcacag agtaaacaag agggaattta gaagttagac aagttagaat
     1381 agcgcctaga ttaatcatga gcagctacta agggatctag gctttgacgc aaactcatgg
     1441 aataaggagt aggtcttctc gtaatgggga ggacgcacaa gaaattgtgt cgtgtgctgc
     1501 agtacatgta gatacttgcg aagacgttga tctttggatc aatcgagcat ttaccgtttt
     1561 ctactagggt ttctcgtttc ggacgccata aacgagtcga tttattttat atatttttga
     1621 gtgaacgaaa aacgataaaa atattcgtta tgaagtaaaa ctaatttagt agatagcaaa
     1681 ggtggtagag gaatgattca aaaatttgcg aaaatgagta aaactgcatg gattgaaaag
     1741 cttgaaaatg acttgagtgt catgcgatta gctccaaaga aattcaaacg agagattgca
     1801 gaaaaagaag caattttgtc ggtattgaat gcggtagacg ataaatgtcc agtaaaaatt
     1861 agtttttcag atatagcaac aattatcgaa ggtatttatt atggtgaagt tgatgaagag
     1921 atatcttcta caaaagattt attagatttt ttggataaaa acaaagatac ctttatagta
     1981 ctaactgatt atgaggaggt tacaacaaat gaaaaattat gatccgaata gccgatgggg
     2041 aacacatacg attaaggttt ctttccaacg ttgggattac aaaggatttg tgacgtttag
     2101 aagaggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg atttatacga
     2161 tcaaaaattg actgataatc caattggatt cggcttgcta cctgaagatg atcaaggtaa
     2221 tgaatggttt aaaatgacgc ttatgaatga taatggagac gaattatctg ttgaagatac
     2281 ttggtcatat ttgagtgatt atattgtcag cgttgaaatc attgaatttg tagcggataa
     2341 agaagaataa aaactggagg aataaatttt acagataaag gcttagaagg agtcacgacg
     2401 attaataaaa aggtagtatt accttaggca ttgagtaagg aatttattta ggaggaaaat
     2461 tagatggcgc gaccagtttt agaggcgatg gatatggata ctttgacaaa aggaaggtat
     2521 gtacagctgt tagctcgtgg ttattcacct aaagaagtat tcaaatccct gtctaaagga
     2581 acagatatgg aaaaagaaag attacgaaaa gaattcgatt attggagaac ccataatgga
     2641 atcaaggatt tgaaaccagc acggccagta attaaacgaa agaaaaagat aacccaagac
     2701 tgaagtgtag agcgtatgtg atcataggct tttttatttt tgcaaaaaac aaggaaggta
     2761 ggaaaactca tgaattatga agaaatcgaa aacagaaaaa aagtgtcgaa agaaatggaa
     2821 gaaaagttgc taaaaacgat gaaacaaaaa caccttaaaa gattatctgt catgcaatat
     2881 atcaatgata tgcagataac aggaaaagag aaagcttgtt tgttaggttc aatgaaaaac
     2941 tttgaacagt tgagacgaac ttatgtgaaa aaaagttcta attgtcaact attgttagaa
     3001 gtctcatgac attcattaat agaattcatt tttaaaactt tttctatgga aatgagaaaa
     3061 agattttttg ttttgtctga aagggggtag ctggatgaga aaatggttat ttggtttttt
     3121 aggagtcgct ttaatcgtct tttgcagtgt gtttggctat gttagctacc aaaaacatga
     3181 aggaaaggtg tttaagcaaa atatagaaaa aaagatgccc gtagatcaaa tcaatgaaca
     3241 tgcaaagagt tataaggagg atgcaacgaa cgtcaataat gatatgtctt tggggcaaat
     3301 gttgtccata caaaaagaag caatcgaaat gggtgtcaat aaacaagcct ttgcacaaat
     3361 tcagatacct gcacttggtt tagcattgcc aatttttaaa ggggcaaatc aatacacttt
     3421 gagtcttgga gcggctactt atttttatga agatgccgaa atgggcaaag gaaactatgt
     3481 cctagcaggt cataacatgg aaatgccagg tgttttattt tctgatattc aaaagttgtc
     3541 attaggggag gtgatggact tagttagtaa cgatggcgtc tatcgttaca aggtaacgcg
     3601 taaatttatc gtacctgagt actttaaatt aatcgatggc gttcctgaag agaactcttt
     3661 cttgtcttta ccaaagaaag gagagaaacc cttattaacg ttgtttactt gtgtttatac
     3721 gtcacaagga aaagaaagat acgtggtaca gggagagcta caataacaag gttttaatca
     3781 acgcgagtcg ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa
     3841 aaaaagctgg aatgcttgtt ggaatgttag gaatcattgc aagctgcttt caggggtatc
     3901 aagtacacgc agaagatgtc acgcaaaaaa caccaccaga gaaggtcaat atcacggttc
     3961 ataaattaat gtacgatcaa ggaacgcaat tgaatgttga tattgacggg atcaaaaacg
     4021 atggttatac gcacgatcaa tatcctacag gtgtaacgaa atacaacaag gcagattatg
     4081 gtgatgttga gtttacgtta ggaaatatta cggatcaagt attaccgaga gaagattctg
     4141 atctaaccaa cgcgaaagtg gacgagattg tacaagatgt agaagacaag ggatctaaca
     4201 gtgagtacgt caagaatgca accaacaaaa ttacttcagc tgtagatgaa aatggtgaga
     4261 tcacttttac tgatcaacca gcctatgtca attccaaagg gaatgtatat gtggtttatg
     4321 aaagtaaatc cgctgctgga ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc
     4381 caatgacaga taaaacagga tcaggatttt taaaaaatat tcatttgtat cctaagaaca
     4441 ttgtttctaa gttgagtttt gaattaacga aatttggtga cgatggcgca gcccaaagca
     4501 aacaaaccct tttaaaaggg gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa
     4561 caaaattggg tgatcttgta tcggatgatc aaggaaagct gactgcgact gatttgactt
     4621 taggaaaata ctattttgtg gaagttcctt cggaagttgt agtgggatca gatcaagaac
     4681 caacagcaga tcaatattta ttaggcgcag acgctcgcaa cgatgcgcat aataaattaa
     4741 cctttgaaat taccaatgac ggcgtgacaa gtgacttaaa agcttcctac gtgaactata
     4801 aagcaccagt gatcgataaa acagtgacaa atggaacagg acaagaacat tctttccaaa
     4861 ttggtgacgc agtgaattat caaggaacga ttcatatccc tacagatatt gcaggtggaa
     4921 cagatgggat tacagtaaat ggtgtgaaga gtgaaacttc tccttacagc gtatttaagt
     4981 ggggagatac agcaggacaa ggactttctt atgtcgctgc taaagcaaat atcaaagtaa
     5041 caaataaaga tggatctgtg gtattaaaag aaaatacaga ttataaaatc caaaattcag
     5101 aaaatggctt tgtgatcgat tttatcgtga ataatggcca agtaagtgat actgtagcaa
     5161 gtttacacgg acaagatctt caaatgactt ataacatgta tgtaaatgac agcgcagctg
     5221 ttgccaatcc attaacaaat agcgtggatt tcgtttataa caacaatcca tttaatcagg
     5281 aagaacacca cgaaaaaacg aaagctgacg ttgtgactta tggtgcaaaa ttcttaaaag
     5341 tcgatagcgg actatttgga acaggaatca aagcaacacc gcttgaaggc gcagaatttg
     5401 ctgctaaaaa cgcagaagga aaatattatg gtggattagt tgatacggat aaagacggtg
     5461 tcaaagaagc agtatgggta gacgatgtag caaatgcagc gatcctaaaa tcagataaag
     5521 aaggtcactt tgaaatcact ggtttaactg aaggtgaata ttcactggaa gaaacaaaag
     5581 caccaactgg ttaccaaaaa ttaacagaag atattgcatt taaagtaaac aaaaactcat
     5641 ttaaagagga aaatcgaatc actgttaaaa ataatcaaaa agcagctatt ccattaacag
     5701 gtagtaatgg tttccaaacg tatgtattga tctcttgctt gttgctagga gcaggggcac
     5761 tttctgcagt tgtttacttt aagaagaaag cctagaagga tcagctaatg attattcgta
     5821 agcccccaaa aagaagagtc atgcacaaac tgatgatatt tttttggttg ctgtcggcag
     5881 tagcagtcta tttaattcca gctgtggaaa caaatgtagc gaaatttcaa caacagcaaa
     5941 gaattttagc cattgaaaat cttgaaaaaa cagattcttc tttggatgtt gcgaaaatag
     6001 agttaggcga tcctgttggt gttcttacaa ttccaagtat tcctttaaaa ctaccaattt
     6061 atgacggaac ttccgacaaa attttagaga atggtgtagg gattacggaa ggaactggcg
     6121 atattacagg aggaaacggt aaaaatcctt taatcgcggg gcacagtgga ctttataaag
     6181 ataatctttt cgatgatttg ccttcagtaa aaaaagggca aaaattcttt attaaggtaa
     6241 atgaagaaca acatgcctat caaatttatc gaattgaaga agttaaagca gaagaattac
     6301 aaaaaaatta tttagattat ctcatgcctg aaaaagacca agataggatc acgttaatga
     6361 catgcacgcc aaaaggtgtt aactcacacc gctttttagt gtatggtgaa cgagtgtcat
     6421 tcagtaaaga agatttaaaa ctacagaaaa aagcaaaaaa ataggaaatc aatttcattg
     6481 gattggcgat tagcactgct attcgctttt tttgcgttcc tattattttt atttataaaa
     6541 aaataaggag gagatcggaa tgaataagag aaaatggctg tttgtttgtg tagcagctct
     6601 ctttgcaact gcagcttact cacagatatc ttttgctgat agcagtacta ctccaactac
     6661 cagtcagaca acagatagtt caacaactgc aacaagtgac acggacagtt cgaagaacac
     6721 tgcagaagaa acggcagatt caagtacgag ttctactgaa tcagcggaat caacgtcaga
     6781 aagttcttca gagacttcag aaaaactgca agaagtagaa gatacgcaag taatcaagca
     6841 aaacattcat attcaaaaaa ttatttcttc gaatcgaatc aacaatcaag gaaggcaagt
     6901 gactcagcaa gaaggtgtca atggtgcaaa gtttattgtc tacgatatca cgaatatctt
     6961 agaagaaatg acacaagatg ataatgagaa aaatcaatcg gtagaaataa ttgagtcaaa
     7021 attgaaagat cgagcaaaaa aactttcata cgatcagttg aaaaaagtga ctgaaggaga
     7081 aacccaaaca attgatgatc aggaaggcgt gattgatttt tcaattgaag tacctgcaaa
     7141 caaaaaacaa gcatactata tcgtcaatga atcaagccct gaaaacattt caaatagcga
     7201 agatattgtt ttattgacac ctgtttcaga tgaaaagggt gaatttttga cggatgtttg
     7261 gttatatcct aaatcaaaga aatcagagcc gaaagaagaa attaaaaaag ttgtatcaac
     7321 tggtgtaaag aaaaactttt ttgataattg ttgggatttt ttcactcatt tattttttaa
     7381 cgattaggat gaaatgatga aaataataaa aatgttagtt tttaatctat cttttttagc
     7441 cgcaacaata ttgtgttgcg gtttagtttt ttctcaatta tcaatttacc caagatatca
     7501 acgattgttc gttcattttc taaatatcca gcatgcttcc atgccactag aagtgattaa
     7561 caagctctta atcaatccaa tttttatggc tagtttgttt cttcttgcag gattggtaac
     7621 gtgggtatta ggaaaaaaag aatgagaagc ttacaatcgt ttaacaaaat aatagatgga
     7681 gggcattatg gaaaataaaa ttcattgcac tacgggaaga gtatttactt tagtgttgtt
     7741 tttgttcctt cttctttttg caaggggaac tgcagtccag gccttagaaa aagttgatcc
     7801 gagtcaaatc aaagaaaata ataatctggt actttatcat gatagtgatt cattgagaaa
     7861 tatcgaagta actgggatca atggatttaa gatactagat gagtattcaa ctaaaaaaca
     7921 aaaagtactt gcttggccaa aagtcaaaga tttgaaaagc acgtcaaaaa ttgttgccca
     7981 ttatcaaaag gtgggcactt atctaggaaa agaaattgat tgcgacgtaa ctttttctaa
     8041 ttttgtagcg cacgctgggg ttaaccaaga cggattagct aaaacaatta atgggaaaaa
     8101 gtattataat tatttgctct ttgataaaaa catttatcga ggctttttca tgcagtcaat
     8161 caacgttatg aatgttaaac tagattttaa gtatgcagga tcaaaaacaa aagtagcttt
     8221 gagtgaacca cagccagaaa ttaattcaaa aatgagatcg atgagagcag ctggtattga
     8281 gttgaatgat acatttctat ctatttacag tttaaatgga cagtatcagc aacgtgtgtc
     8341 ttggtataat gacaataatg aatctgtcgg tgaacgtaat attgatatga gagaatttgt
     8401 tgggtacgaa aaaatggatc gcttacctta ttttgtcaca acagatacag catgtgcaga
     8461 atacaaagct cctgcgttat caacaaacaa tttaacttca aaagtagtgg gaggacgtgc
     8521 agaaaaggct tatagctcga atgatcattt caccgatgtt gtaggagctg atacttatca
     8581 cagaagtggt gtaacgtata cgcttcaagg cgcttcccca acattcatga ttggcgcaaa
     8641 tacgaatagt atgatgttta gctttgatac tgcattgctt tggacaccac aaccatcgaa
     8701 gcctacaaaa gaagtgttta acaaagctaa tactgaagag gcagcacaca atattgacaa
     8761 aaaagtgatt ccacaaggat cagatgttta ctatcatatt caccaaaagt ttgatgcatt
     8821 aacagtcaac acaatgaaca aatacaaatc atttaaaatc actgatacct ttgacagcaa
     8881 aaattttgat atggtatcgg atgggaaaaa ctatgatggc gcattgtggt atgttgaagc
     8941 gaatgggact tggaaaaaag ctgatacagg aggaaaatta actgtatcag gtaatacagt
     9001 cacttatact gcttctgaca gctttttagc taatgctgca aattacggta gggactacat
     9061 tttgcagctc catatgagag caaaaaatga tgcaaaagga attgcaccaa atgtggctac
     9121 tgtaacgttt aacaatgatc cgaacaaagg aacacaaaaa acaaataaag tagaaaacta
     9181 tttctctctt aaaccaaata aaaaagtgga acaagatggg aaagatgttg atggtcgaaa
     9241 tgatggtaaa aaaggaacgc ctacagctcc tttaaatgca ggatctgaag tacaatatct
     9301 agtcacacaa aaatggcata caaaaggtgt agacattgcg ctcgatcatt acaagcaatt
     9361 tagtattcaa gatccagtgg aagctcgttt gacgtataaa gaaggttctg cgcgagtgat
     9421 agatatgagt acaggaaaag atattacttc agaaggtata ctaacctatg atagccattc
     9481 aagaacgctg aaatgggaag cttccgctga tttcttaaat aacaatcttt tagatggacg
     9541 agaaattcaa ctgatattta cagctaaaac tcctttgaat gaagaattgg atatcgacaa
     9601 ccaagcaaaa gtttcagtag aaagtattga gaaagcaact aatgtagtaa cgattggggt
     9661 aaaacctaac ttacctcagg tcattgtgcc aaaaactgga tcgaatcatt tagtgctaac
     9721 tgttattgga agtatattat ttcttacttt agcatcattg ggctatgtag ttttaaaact
     9781 caaataaata taaaaacaag gctgattttc agccttgttt tcttttattt gaagattgga
     9841 ttcgcactaa taaatacatt atctacgcca attctaccag cagagtcaac tacaaaatta
     9901 tgacctaatc cgagaatact ttggttatta taaagtaaat ttgcattatg ggcagggcta
     9961 tttttccatc ctaccatggc atcgatagca ccatcgtctg ataaaaggtc atcaatatac
    10021 agcccagttt gacttatgtt ttctccctta gcaccatagc agcctaagct ttccaataaa
    10081 tcttggaaag taccaccacc agggcgatag tgactgaagc tgacttgtaa ttctttagct
    10141 cgaatatctg cagcctgttg aacaatatca ttgaattgca ttggggcaac accattttgt
    10201 tggcgataat catttaacca agcaagttct ttttgacgca taaagttacg taattcgtct
    10261 aatgaaatct ttttatattt gaatgtgatt gttttatttt gatcgacgtt attaatggat
    10321 cgttggccgt ttttatccac ggtaacggta ggatcgtcat ttctgcacaa gaaataattt
    10381 tgatcagtca tatttttcca aggagtagag aagctatccc cttcgtatac tgtttttgat
    10441 tcactagaga gaacagcgtt ggtatcagca tttacatgat taacagtcac attaaattta
    10501 cgagtgtagt tgaaaatata attttgactt tcagtcacat tgtttaaggt tactgaattt
    10561 gttggttcta cccgatcgtc taaaacatat ccttgaatag aaggagcagt taaagtgaca
    10621 ctttcaccgt gagtgacgtt gattatctgt tctttcaaaa tatcaactcg cccctcattt
    10681 ttgtagttgt tattgcacaa caaattgatt tgatgcactt gattataatt gaatgtgatt
    10741 gttgtattac ctgtgattgt tttgctttga gtatcattac caatcacttt gtagctaaaa
    10801 ttatctttag caccttcgct atatttaaat gcttttgcct tagcagtgta agtagaacca
    10861 gaagtcactt ttgcttgatc tgtagcaatc acttgacctg tttgattgtc aatatggtta
    10921 atggtaacca tatattcttt tggcgtttca ggtttgtgtt gcaacccttt aaatgcagca
    10981 tcaagactat ttttagcatt gtttacttgt gtttgagaaa ccttagaatc gtctagtact
    11041 tttttagcat tggataaagc agtctgaaaa ttattccaag attcttctgt aaaatctcct
    11101 ttagcagttg attttactcg gttgtataaa gcttgtaatt cagctttatt cacagtagga
    11161 actggttttt tctttaatcc tttgtaagca gaatctaatt tatttttggc actatccact
    11221 tgcttttgag tcgcttttga gtcatttaac actttcttag cattgtttaa agcggtttgg
    11281 aatgcgttcc aggtattgtc ggtatagtca ccttttttcg ttcccttaac gctcgtataa
    11341 agagcttcta gagccgattt attcactttg ggtttagagg tgtcagcgcc ataccaaccg
    11401 ataccttcgt atttccagcc atagtctttg gttaaccatt gtacttcttt ccaacttgca
    11461 gtgtagtgat gtgagccaga ttttacacca ggaaggaaaa ggcggtagat cggtttatta
    11521 tttttttcgg cagaacgcca ggcaggccct tcataagtcc agccgtaatt tttagtcaac
    11581 catttcactt catcccaact agctgtgtaa tggtgatcac gaagaccttt attgtacaaa
    11641 cgatagacaa gatggccacc gctagtcggt gcttcccagg cggggccttc ataatttccc
    11701 cattttatct ttactagatg gtcacgttct tttttgctag aggtgtaaaa atgttcatta
    11761 ttatttggat tgtaaaggcg atacatgacg ttagttgaag cagcgtcaac agatccagca
    11821 agactaagtg aggcagcaaa tgttccaata gctaaaacag tttttggatt aaataatttc
    11881 attttttcaa ctcctaaatt taattaaaaa tgtttacatg attattatat atcggttaac
    11941 cgatatagtc aataaaaaat aagaaaagcc ttatataatt ttggcttttc ttattttatt
    12001 tgttagttga agtattaata tcaacgattt cttcagccaa tttatttaat ttcatagcat
    12061 ttatgaatct catgttgtct atactagagc gtttgttggc aaggtcactt actgtagtta
    12121 agggtacgcc agattctatg gatatagaac gaactgattt accactatca aacagcaacc
    12181 attcgatttt ctttttatca gcgtacattt caatgctcct tcttgttttt tctttattat
    12241 agctcttttt tgtaatgaat cacaatatca agagaaaaat tttctataga aaggtggatt
    12301 tttatgagta ttcgttctcc gtgtatccaa atgaggtttg ttgctacttt cgttatttga
    12361 tatactaaaa ataaacaacg aaagaaggaa taaaatgaag aaaaagcaac tatggtttgt
    12421 ctctatgttt gcaatatttt ttattatttt aagtggatgt tcaagtaata agaaatcgga
    12481 agctaaagaa gataatagtt tattgcaacc aggaactgtt tggaaagaag aagtaggtgg
    12541 tcttattcat aatttaaaaa ttatagatga tactacctgg gaatattctg aaagtgtatg
    12601 gcatccagat cctgtacaaa taacagttaa gaggcaaaaa gactatgaag gtttagaaag
    12661 atacaaaata gtagattctg caggcattag ggaatttata aacaaacctg attcattatt
    12721 tattgttgtt ccctatgaga aggatggcgt aaaaaaaata atttttcttg gatctagcaa
    12781 ggatgagaag cgaacgaaag agaaattaat tcatgatgga tcacaatcaa ataaatacaa
    12841 attacaaaag actagcgagt gattgctagt ctttttattt gtcctgaagg aggaatcaca
    12901 gtggcaaaag ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgccgcggt
    12961 gtatctgaaa cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg
    13021 ggaaaagaca aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc
    13081 gatgaaatta ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga
    13141 attttttaac acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaactg
    13201 ccgcaactag tttgaaaaat aatctgatga tttttgctgc tgttgcggca gtggcttcca
    13261 ttgctcttgg aggtattttc ttcttctttg ggcgaagagg agctgaaaac gggaagacac
    13321 acgtaggtta tgtattgttg ggggtaggag ttattttagc tgccgcttct attgtagttt
    13381 caatgttctc aatgtttgga caaaccccac aaggactata aaaaaacaag tagaaggagt
    13441 aaaaagtacg tggctacttt ttactccttt ttttatggaa ggagaatatt catgtttttt
    13501 acgatttcag gcaatgcaaa attggataaa gagttaataa gaaaagcaac gaagaaagtg
    13561 ggaaagcgta cttccttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa
    13621 cagctacatg tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact
    13681 ctcacgttac atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc
    13741 tcactggatt atcaaatttc agaaggtttt tatcctacat tgattgattt aatccgccta
    13801 gatatattta aagatacgga tgaaaccatg agtgtggaag agtcttatcg aaacgaaaaa
    13861 attttacaag ttttagaaga agagttagaa agcgaccaaa gaaaagtgaa tcaagtgagt
    13921 gaagtcgaag caaacgaaga agtaccatgg gaacgcccaa tctttccaaa ggatgaatca
    13981 aaagaaaaag agaaaggaat tattgacgaa caagctgaca ttttgactca taaatcaata
    14041 tttgaggaag agaacgaggt aatgggtcaa ttggaggtag atccctttga agaaacaatg
    14101 gaggatgaag agacggccga agtagaagtt gatccagttg aagctaatga agacccaaca
    14161 tggactgaag gggattctca ggaatatatt gaaccactta tcagtgaaga agtagagaaa
    14221 aatgaaacag agatacaaaa ggcaacggta tttgattata ttttggatca atatccgcca
    14281 gagcatgaat ggatgaaaga aaaaaataaa ttatttttaa atgaacttta ttcgaaatat
    14341 caattacctc aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt
    14401 aaaggcacac ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca
    14461 caagaagatt tattagctac ttttgaagaa cgtcaacaag aaacagatca agaaattaat
    14521 gagtatgcga cggaacagtc gtcgaattgg gagaaagcaa aagaggaact aaataaagaa
    14581 gaacagcgag tgattgaaga agaagtacaa aaaattaaag agcggtacga aaagaaacgt
    14641 gacaatgcgt attccttgtg tgtagaaaag atcaacaatt ttactcagac gaatcaagaa
    14701 gcattatcga aagaaaaaga aaacttatta aaagaacgta taaaagaacg aaaagaagaa
    14761 ttttaccgag ggttgcgtac tgataagcta aggattagca aagaattaaa tgaacaactg
    14821 aataaactct atcaaactac gactgaagtt ctgatggaaa aacacgagca gatccaacaa
    14881 gaattacaag cacaaattcc aaaatggaaa aaagagtatg aaattcacca acaagaattg
    14941 aaagaacaag aagaaaaaca acggaaaaaa gaaaaagagc gcgaacaatt agaactaaaa
    15001 aaacgtgaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gctagaacaa
    15061 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcga
    15121 caagcacagt taaattatct gacacatcct caaccagtag ctgctgttca aacgccagtg
    15181 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
    15241 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    15301 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
    15361 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    15421 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
    15481 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
    15541 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
    15601 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
    15661 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
    15721 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
    15781 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
    15841 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
    15901 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
    15961 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    16021 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    16081 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    16141 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    16201 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    16261 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    16321 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    16381 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    16441 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    16501 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    16561 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    16621 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    16681 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    16741 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    16801 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    16861 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    16921 taggctataa aggatttagt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    16981 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    17041 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    17101 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggaaaaaagt aacttattag
    17161 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    17221 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    17281 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    17341 tagatgaatt tgggaatagt gtgacctgtt tagcctaagg ataaaagagg ctagttccaa
    17401 cgaacattgg aataacttta ctatcgagaa atcaaatgac ggggtaacgc cctgaagggt
    17461 ttccactgat actgcgacat gcgttagtag gatgtgcatc caaacgcgta tgaagctcgc
    17521 tgaagtcggc agaagggtac cgtaaggaaa gacacgatac gtatctacca cgcaagtgct
    17581 ctagaggtag ggtatgtacc tgatatgtcg ggtgtcctaa aaagttcaat aaggtgagaa
    17641 tgatttgatt atctgacaaa cttgcgaatg gaatgtctga aacgaataac tcatagaaat
    17701 gtgaggggga agggggatac gtcctctttc cattgactcg aggttgagta aaaatgtgtt
    17761 tctatgaaaa accttgcatg gttaaaggct atggagtagt caacaggcaa aagcaagcac
    17821 ctaagttgaa atctatggat agatagtaag gaacagggga agcatctagc acggagggag
    17881 cataccgaag atgtgtttct aaggaaaaac cgaaatcggt tataacttag gtgaatagat
    17941 gtgaaagtag agccacgacc agtgaaaatt tcgtaatgaa attggaggaa caggctcaag
    18001 tcgttattta taatataaga aacctattta aaatcatgga ttctcgtaag actaaaaagg
    18061 tcacgaacca gaatggagcg tgataaaacc atgactaaca caaaaggaat tagcttaaag
    18121 aacaaacaac tcagacacgc cgaatactac gacatgactg agactttcga tgaattgcac
    18181 gcaatgagtg ttgcaaaatc aaattttaaa aatctcatga aattcattat tgatgacaat
    18241 aatatctttc tagcgtatag aaatatcaaa aagaataaag gaagtgcaac ggccggatac
    18301 gatggtgtca ctataaaaga cattgaaaaa ttggcatgtg gtgacttttt aactattatt
    18361 aaaaggcgat ttaaggatta cagaccaaga aaagttaaaa gagtagagat cccgaaacca
    18421 aacggaaaac ttcgaccttt aggaattcct tcaatatggg acaggatagt ccagcaatgt
    18481 attctacaaa ttttagaacc aatatgcgaa gcaaaattta ataagcatag ctatggattc
    18541 agaccaaatt gttctacaga aaacgccata gcagattgtc ttgctagaat caatcaaagt
    18601 aagatgaatt atgttgtaga tattgatata aaaggattct ttgacgaagt aaaccatacc
    18661 aaacttatgc gtcaaatatg gacaatgggg attcgagata aacaactcct tgtaattatt
    18721 cgtaagatgt taaaggctcc gattgttatg cgaaatggca atgttgtttt tccaaccaag
    18781 ggtactccac aaggagggat tttaagtcca ctgcttgcga atatatgcct gaatgaattt
    18841 gattggtgga tagctaatca atgggaggaa cgaactgtca aagagctgaa acaccactat
    18901 cgaaaaaacg gagcatggga taaaggaaat atctattcga aattaagaag ccacacaagt
    18961 ttaaaagaga tgtatctggt acgctatgca gatgatttta aaattttctg ccgaacagga
    19021 gaagaagccc aaagggcttt ttatgccaca caaaaatggc tcagagaaag attaaagctc
    19081 ccaatttcaa tggagaaatc taaagttacg aacctaaata aagctgaaag tgaattcttg
    19141 ggtttttcaa taagactcca caaaaaggga aaaaagaaag tggctttcac gtacgtttgt
    19201 cgcaaagcag aagaaaacat taaaaataaa ctaaaacaac agattaaaaa aattcaatac
    19261 gctggagcaa aaaaaaatag gcaccgagca ataggtgact acaacatgat ggtcatcggt
    19321 atccacaatt actatcggat agcttcacat tgtagtgtca atttcactga gatttataga
    19381 agtgttagga cagtaataga agcaagactg aaagctagag gaatgacgaa aaatggaatc
    19441 tatggacaag atcatgaagg ataccgtccg tatctaaagt ctaagcaaat gagatatatt
    19501 gaaggttttc cgatacttcc aattggctat attaaaaaaa gaactccact caacaagcgg
    19561 agaaatctaa ataaatatac caaggaaggg agaaagctca ttcatcaaga gcaacaagct
    19621 atagaaagct ggaaaatcaa gtatcttgta aaaggtttta gtaacaataa gaaatacacg
    19681 gtggaatttt ctgataacaa aatatctaag tttatttccc aaaaagggaa atgcgctatc
    19741 acaggaaacg aacttttgat ggaagaaatt gaatgccatc acattaaccc atatcatttg
    19801 tccaaagacg atagttacaa gaaccttgtc attatctcta gagaaatcca tagattaatc
    19861 catgcaacta gtgaagaaac tattctgaaa ctgcttcggt tactgaaact aaatcactat
    19921 caaattagaa aactaaacac gtatcgaaaa aaagcagcta attttccaat atcaatcaaa
    19981 atgaattaga taggaaacaa attatattgt aattaacgat ggaacgccgt gtgcggtgaa
    20041 agtcgcacgc acggtgtgga ccgggggaaa agacggagat aatatcaaac tcttacctat
    20101 cggaattgcc accaatggat aatatggaag gaatcatgac ggtcacagct ggccgaaaca
    20161 tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat ggagaacaaa
    20221 acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg acagatgata
    20281 ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa gagaacagta
    20341 ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca gatcgtatct
    20401 taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc gctcctttaa
    20461 aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat catggggaaa
    20521 cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg gatatcaatg
    20581 atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa attgattatg
    20641 attatttcct accagatgaa tatgctagaa atctctatca tggactgcat gatcaattag
    20701 aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag aatgagacgg
    20761 ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt caagaatatg
    20821 ttgaaaagtt gatcaaaaat gaagaaattt ctgaaatgat tgggaaacgg ttgttattgg
    20881 aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca tagccgactg
    20941 gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga aagtcttaca
    21001 agattatggt aattatctgc agtatcaaga tgtgttcagt tggatttgta atactttttt
    21061 atgggggttg atcaaagggt tatataatct caatacttta ctagaaaaaa ccatttatca
    21121 aagctttgat ttaaaagact tattgaacgc cgctggggta aatgaactat ttcaaagttt
    21181 gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag gcttgaaatt
    21241 ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat ttatggccat
    21301 gatcttgatt tttggtggta gttctttaat cgatgaaggg ttaaatatca gtaaaagctt
    21361 ttacggagac gtcactgcag caaataaagc agataaaacg ggttcacctg cttttcaaat
    21421 tattcaaaat aatgtgatcg atgtcagtac attactagca actgacccga ataaagtaga
    21481 atccctgcca acagacaaaa gaaatgcttt aacagcgaaa aattttaaaa cagcgaatat
    21541 caatgggatt attactccta aacaagctaa agaaatggcg gataaagata agagtggttt
    21601 atcagattta caaaaggatc ggttaagagc gttacaatac cgactggaac tggatgataa
    21661 tggaaaagaa attcctgtgc aaatcagtga tgaaggctta gccaaatatg tttatacttc
    21721 agggtatcgt cgatacatta gtagtccagg gattatttta gctggtgaat cttctttggc
    21781 ggtggcctat ttatttatcc tatttacgat cgttacttgc attattgagc tggtatttaa
    21841 aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggacaac ggatgaaaag
    21901 tgctatcgaa gatatttctc aatccttctt gttattggca tttacaattt tagaattacg
    21961 aatctatacc ttaatgcttt caggtattgg tgatttgcat gcggcaggaa agatcaatgg
    22021 attcttgtat gttgtcgctt tgattgtact aaccattgcg ctattcaaag gctctcaatc
    22081 agttacgaaa attttcggcg ttgatacgag tctgaaaaac ggcagtaatt ctcttcttag
    22141 catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa accttgcaag
    22201 tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat ttggccatcg
    22261 aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta caactgtacc
    22321 taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg tttctaaagc
    22381 cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg aagatcttac
    22441 agaaaaagcc aaagacacct tagaagataa attagacggc acgagagcta aagaattggc
    22501 tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag cttcagaagt
    22561 caaagaagga ctaaaagatg cgtatggtga aggacaagtc aatgcagcag tgaaagctaa
    22621 tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca aagatcacca
    22681 agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag aagaaagaaa
    22741 ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat atcgctataa
    22801 acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag taaatcaaga
    22861 agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat atcggtatag
    22921 atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa atcaagaagt
    22981 acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag ttggaactga
    23041 aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag atagtcgttt
    23101 gaatcaattc aaggataatg atcaaagttt tgttcatcaa tcacctgcag gtactgttca
    23161 aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt acaatttatc
    23221 tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag aagctgcacc
    23281 aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat ttactgcacc
    23341 aacaaaaata aatgtgacaa gagaaactaa aaacaggaac aatgcattga aagaacaact
    23401 attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag gccttttctt
    23461 tatatgaaaa ggagaaatca attatggata aatatataaa catgaaaaac cctccaagat
    23521 ttagtggaga atggaagttt ttcgcttttt tctttttaga tctattaacg cttggtggct
    23581 gctttttttg ggcgaaaatg gtcaataatc ttgtccactt accactcttt tttgcactct
    23641 tgaattatgg cttatggatt gcgatagcgt tagtgattgt ttggcgaccc tataataacc
    23701 caggtcaacg acaaattttt gcgattatcg agtctatttt ttttgcggac aagaaccatt
    23761 atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat gaataaatgc
    23821 gagtcaaagg acaaaattca gttgaaagga aatccatttt gcagtttgga aaaccagaag
    23881 agcgctctat cttggactta tttccgtata aatcctgtac agaagaggga gtcttaatta
    23941 ctagagaaga tcattttcaa cgttattatc gtgtagtttc tacagacgtg gaaggattaa
    24001 atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt acgtatgtgc
    24061 caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa atcgtgcaaa
    24121 agcggcagtt attagataaa aatcgtatgg aacagtcctt aggaagaaat ctacagcgat
    24181 taagaaaata tgaaaagaaa ttagtagagg aaatccagga attgaaacga gcagaacagg
    24241 aacgccctga tttaagcttc tttttcgtca ttgaaggaaa gacaatcaag gatttgcaaa
    24301 ccagaaaccg tcagttaatg cgatctagtg gcattttagg gttaaaacca ctctacaaaa
    24361 aagaattgat caagattctt tatcgaatga ataatatgaa tgatgagtag gaatggggaa
    24421 gagagaatga aatatccgtt gatatggtta gaaaaagatg ttccgaaaga agagaccact
    24481 agaaaatttc ctcacacttt aagcctgggt aagttgggaa aaggaaaatc ttttaaccaa
    24541 gtaaagaatc aaacaagaag aggtgactgt acatgttaga agcaattaaa acagcttttt
    24601 caccacaaaa tgatcaaaag gtacttgatc cattaaagca actaggaaat cgggaaatag
    24661 aaattttaaa atccaaaggg tatgatttag actttttaaa acaagtgatg ccacaaggtg
    24721 gattaaattt cgatgaagag tatgtgacct atggaaatgg ttattcacga tgtgtgacag
    24781 tttatcacta cacaaaaaat cctacgcttt tttggttggc caatctcatg aacaatcctc
    24841 atacagtggc aacactagat gttagcacag atgaaaagca aaaagtgatt gatcaaatca
    24901 atcgtgcctt agatgaatta aatgatcggt cagataatga acgaaaagcg actgaccgaa
    24961 ataaaagcgc ggaagaatac ctagaactag cggaatatgc ccgaaaattg acttctaatg
    25021 gggaaatttc taaacaagtc aaagtgagag tacatgttta cggaaacacg attcaagaag
    25081 tagatgaacg agcacaggaa ctgatcgaaa atctcaaagg gatggattat cgtgcagtga
    25141 cctatctgca caaaacgaaa agagactggc aatcgatgtt caccatgttc gatgaacaag
    25201 ctgaatggtt tggaaatatc aaagggcaat cactaccatc gctaaacatt ggcggaggtt
    25261 tcccgttcca ccaccaaacg ctaaaagatc caggtggaac acaatatggg gaaaccatga
    25321 caggcggccc gtttgtttgg aatgccacgt ataaaaatag tgtgcggttg tctaccaata
    25381 ccttgcttct aggaatgatg ggagctggta aatcgaccgt tcttaaaatg atcgcggaag
    25441 cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac tttattccct
    25501 ttttaaagga atataacggc attatggtgc gtttagacgg atctgacggg atgatcaatc
    25561 ctttagaaat atttgccaca cgtacgtatg atgaagcttc cagtttatat gacgatggat
    25621 cagtcaaaga tttgaagatc aatgaagcag cgtcctatca aacgcactta gacaaagtgg
    25681 tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca gaatttaaga
    25741 cctatttaaa tcgtttttat gaagagtatg gaacagtacc acgcggcttt acttcctcaa
    25801 attcgcgttc aaatactgat acgcaagtca caggtaaaga tccagaagct tatccgacgt
    25861 tcaaagaatt tttagacttt ttaggacaac tggaattacc aggtgcttcc caagagaaaa
    25921 agaatcgaaa agaagaaatg gaatccatcg tagaatcctt atgtgagacg tatggcatga
    25981 tttttgatgg tcatagtacg attcgacacc ttgaccaaca acagttggtc ttttttgaca
    26041 ttgactctat tacaggctta gatcgagggg tacaacaatg tcaaatgtac atggctatga
    26101 cgttgatttg gaatcaggca ttgattcaag gaagaagaca acgacgttta attaaagaaa
    26161 aacaaatgga agaacaagac ttcaaatgct tcttggcgat gattgatgag tgccataatg
    26221 gtgcgacctg ttctgcagtg aaaagctgtg ggttgcaagt agtctaaaaa ctattgtaat
    26281 aactcttata acgagaagtc aaatgagtgg gtaacgccac aaagggcttc cactgatact
    26341 ccgacatgca acaagtagtt tatgcagacc aacgttgtgt gaagctcggt gaagtcggct
    26401 gaaggatacc gtaaggtaaa agaaatagtg aataccacga aagttctcta gaggtaaagg
    26461 gtgtatccga taagtcgggt gtccaaaaaa acttaatatg gtgagtatgt acaaacgtac
    26521 tgacgaacta gcgaatgtac tgtccgaaag gcatagctcc tagaaatggg agagacagtg
    26581 agcaaaaaat cactcattgt gctaacttgt ggttgagtaa aagtcgttgt tatgaaagac
    26641 catgctgcgt tacaggcgta ggagaagtta gggggcaaaa gctagcatct aagttaaggc
    26701 atgtggatag atataaggga acaggggaag ccatcaacat ggagagtgca tactcgatga
    26761 agtggtagta agaaatatgt gaatgacaaa taacttactt tagtgatggt gaaagtagag
    26821 tcacgactga tgaaggttct gtaatggaac tggaggaaca ggctcgattc gtctgtaaaa
    26881 gtcatgaaac ccgttcaacc atgggattct cgtatgacta aaaaggtcac gagccaattg
    26941 gagcgtgata aaccatgaca aaagaaacaa gtccaaaaga taggaagttg cggcatgcag
    27001 aatattatgg catgacggaa atcttcgata agatatactc caaaagtttg aagggagata
    27061 atttcaagaa tctaatgcag attatcattt ctgataacaa cattgtccta gcatatcgta
    27121 atatcaagag aaatcacggt agtgtgacaa gaggagaaga tggagtaacg attaaagata
    27181 ttgaaaatct caaccgtaat gaattcgtag aaattgttag gaagcgattc gcacattata
    27241 accctcggaa agtaaggcgt gtagaaattc ctaaatataa tggtaaatta agaccgttgg
    27301 gtattccatc aatatgggat agaatcgcgc agcaatgcat cttacaagta ttagaaccaa
    27361 tatgtgaagc aaaattcaat aatcacagct atggtttccg cccaaataga tcagcagaac
    27421 acgcaatagc agattgtatg gcccgaatga atcaaagtca catggaatat gtggtagatg
    27481 tagatatcaa aggtttcttc gacgaagtca atcatactaa actcatgaga caaatctggt
    27541 ctatgggtat acgagacaaa cagcttctct taatcattag gaaaatgtta agagcacccg
    27601 tagtacttcc aaatgggaag ttgatatacc caacaaaagg aacaccacaa ggcggaatac
    27661 ttagcccatt acttgcgaac atcaatctca acgaatttga ctggtggata gcaaatcagt
    27721 gggaggaaaa agatgccaaa gagctaaaac atcattttag gaaaaatgga gcttgggata
    27781 aaggtaatat ctatcacaag ctcagaaaga gtacaactct aaaagaaata tacctagtga
    27841 gatacgcaga tgattttaaa attttctgta gaaattataa tgaagcgaaa aaagttttct
    27901 atgcaactaa aatgtggtta caggagcgtc taaagttacc aatatctgaa gaaaagtcaa
    27961 aaatcacaaa tctttcaaaa gaagaaagtg aatttttagg ctttacgatg aaattagtga
    28021 ccaaaggtaa caaaaaggtt gttaatacgc atataggtac aaaagcacaa agcatgatta
    28081 aagtgaaact aaaaaaacaa atcaaacgaa ttcaaaaaag tcctaatagc aaagatacct
    28141 ttatctatat tggagtctat aacagtatgg ttattgggat tcataattac ttcaaaattg
    28201 cgactcattg tagtgtagca atgcgaaaaa ttgcaagaga actcagacca agttttgaaa
    28261 tacgactacg agaacaagga atctcaaaag aaggacaata cggaaagtac gaaggataca
    28321 agccttacct acggtcagga caaatacgat atataagagg gtttccaata ttaccagtag
    28381 ggtatattca acacagacat ccgatgtgta aaaaaagaaa tattaataaa tacaccgaag
    28441 aaggtaggga agccattcat aagaagcaac aaaccgtaga agaatggaag atatcgtggt
    28501 tgtgtagcaa tccagtgctt agtaagagag caacgattga atttgcggac aatcgaattt
    28561 caaaattcat cgctcaaaag ggaagatgtg cagtaacagg tgaggagctt atcttatctg
    28621 aaatgcattg tcatcatatt atcccttatc atgaaagcaa aagtgatagc tatgaaaacc
    28681 tagtgattgt tactgaggag gttcatcggg taatacacgc tacacaaagt gaaacgattg
    28741 aggaacttct aaaatatcta aaattaaatc caaaacaaaa agaaaaactc aatgagttga
    28801 gattaaaagt tggtaacgaa gaaatatctt aataaatatt tgtaggacgg gaaaacagaa
    28861 tgtcttttat agacgaggga acgccgtatg cggtgaaagt cgcacgtacg gtgtggactg
    28921 ggggaaaaga cggagataat atcaaactct tacctatcag tatcgattaa tccagatttt
    28981 attgaaacgg taaattatgt cacaaacttt caacgagaaa tgcgtaaagt agaagcaatg
    29041 actgttttag ctacacaatc cccacaagaa atggtgccag aaaatatgtc ttctgataac
    29101 ttctcgaaac tgaagaaagt ctttgaattt tcttctacga agatctttat gcgtatggat
    29161 gaatctattt tacgtcatat agaaggatta gttggtaaat caatgacaaa ctcagaatta
    29221 gaatccattc ctcaacaaaa tcaaggagac gctgtgatca attttggctc aaaagaaacc
    29281 attcgtgttc attttagtcc gaacaaacgt caattaaaat tgtttgatgg aggaaaataa
    29341 tggaaaacca aaatgaaagt ttaatcaagc agtatgttaa gcgtcgtgca aagcgacgct
    29401 tatttttgtg gttgtttggt acttctgcag gtttgattac tattttgatt actgtgttcg
    29461 tgaccctttt tctaatactt gcggcgggtt cgattgataa ttctgattca gattcaagta
    29521 gtggtggcga agcttttaca ggagaatatt cggaaggctt accgatttat aaagaaatca
    29581 aaggaagagg ccctttttct gatgagattg cgcaatatgc tgtaggtgca gcagtgaaat
    29641 ataaattatt accaagtgtg atcctttcgc aatacggtta tgaatcagcg tttggtacgt
    29701 cggcgtccgc aagaaatgat ctcaactatt ttgggattac ttggtttgat ggttgtctgt
    29761 ttcctaaagg aactgcgcga ggaatcggtg gtattgaagg tggctggtac atgaagtttc
    29821 ccaactcaaa agcagccttc tcctattacg gctttatggt agccacacaa tccaatttta
    29881 atgcatgtgt aggaaataaa agccctggcg caagcttatt gatcttagga cgaggtggtt
    29941 atgccgctgc aggaattact gaagatagtc cgtattatac aggttgtatg tccattatca
    30001 cttctaataa attaaccgaa tatgatgaat tcgccatcaa acattggggt gaaggtggca
    30061 ataacaacgg cacgatcact ggtgaatgga cgaatccatt ccctggcagc agtttagata
    30121 aaagttcctt cagtggtggt caattatttg gaacaaatcc aggcggtgaa ttccgtccga
    30181 atggcttcca tgatggatta gactttggtt cagtcgatca tccaggaagt gaaattcacg
    30241 cggtgcatgg aggaaaagtc gtgtacgttg gcaatcctgg tatttcaggt ttaggtgcat
    30301 gtgtgatcgt gattaattac gacggtttga atatggtata tcaagaattt gcgaatagta
    30361 caggaaattc tcgtgtgaag gttggcgatc aagtgaaagt tggtcaagta atcgggatac
    30421 gagatacagc acacttgcat ttaggtttca ctcgaatgga ttggcgtcaa gcacaaggac
    30481 acgcattcac agacgatggt acatggattg atccattacc atctttgaat agcagcaaaa
    30541 aatagatcag gagaaaaaat atggaaaata aaactttaaa aatcgtctta acagctgcaa
    30601 ttagcgttag tttaatcggc aatatcgcgt tgttttggca gctgaaagaa gaacaagaga
    30661 caaatacctt taaaattgaa gaagtggcta aaaacgcgtc tgagaagctc aaaaaaaatt
    30721 cctctgaggc acaggcgaag atcgacaagt taaatcaaac catcaatgag aaaaaccaag
    30781 aaatcgatca gctgaagcaa caagatggca caaaagcaaa agaggaagta actgaagcac
    30841 aaagaaagac ggcagaagag ttcggtcaat tggccattga taaatcatta agtagaaatg
    30901 aattaacgga aaaactaaaa gatgtcgcaa cccaagaagt gatcgacaaa ttaagtcctg
    30961 cagacgatga tcaccaacat gatgattacg gaagttactc ctttacatta ggtgacgtgc
    31021 aaacttatgc tcaaacaagc agtgtaaaag aggaacaaaa ttttgtcgta tttgttgatt
    31081 atacaatcgg caatccgcaa ttcaaagatg tcaaaccaca aaaaataaaa ggtggattaa
    31141 ctgtcactga aaaacaagta gatggtcaat ggaaagtcat agactttagt tatttcactc
    31201 gttaggagga agaaaatgag taagaaaaaa ggagaaagac aacgtttcaa aaaacgaaaa
    31261 agttatcttc aatggctggg aatctttatg attctcggct ttttgattct tgctagtccg
    31321 atttatacga atcagaaggt agatatacca gatgaggtaa gcacgaacgc ctttttaaac
    31381 acgaaagagc tgacaatggt ttcaaaagaa aaaggagcga accagcaatt agtttttgaa
    31441 tttgcgatcg atgatgaaga tcaaagcttg ttaaaagaac tggctaatct taattatcgc
    31501 gtagaagtaa agacagctaa aggggattat caagcaatac gagccaaagt gatcaaggtg
    31561 tctgatgatt attttgcagt gaagctaacg catgtaccag aagaatatat cgccatgcgt
    31621 ttaaccatta ttcctgaaaa aattgatcca aaagtggata tgcaagaacc acaagatctg
    31681 atttattacg tgcacgaaga taaggttaaa gatcaagtga aagatgaaga ttatgaacaa
    31741 cacgcgatca attacaaagt gaaaggatac aagaaagaac aaaaagcagc tcaaaaacaa
    31801 atggaatctt tccaagcaac cattgatttg aatgaagaat tagtgaagaa gctaaaagat
    31861 caattgcctt atcaagtcgc agatgatcag gaaaatactg aaaacaagat taatagctac
    31921 cagcaagaaa ttgaaacatc gaaaacgcaa ctgaaagatc aagaagagat cattcaaaaa
    31981 cttcaagaaa aaattgatcg tatgacaaaa gaaaagattt agaaggacag gtggagtgaa
    32041 aaaatgaagc gaacaaactt ggctgacaaa gatactttcg ctgagcagaa agtattgatt
    32101 aaagcctatc tcaaagttgt aaaagctaaa gaaatatgtc gagaagtgaa tttatctaac
    32161 acaacaatat ctgagttgaa gaatggtcga aagaatttag atactttaaa attcagatca
    32221 tttgaagctc tatatgtagc tgctaaaaaa tattttacag ctgataacaa ataaaaaaca
    32281 ggagtatttt atgtgaaaaa agtatataaa aaatgcttat gaatcagcta tatcacctta
    32341 accatttgtt aatgagatgg gagaaaacat taaccacgat tctaacgcaa acatcaagca
    32401 cataaaacat ttaacactcc tcctttctgg ctgaagattt caaccaaaca ggaagacagc
    32461 gtatcaatta tagcatacac tttttcttaa attcattttt catatgacaa aatatttcct
    32521 tctaatcgac aaagttagaa ggatttttta ttgtctaaat acacaaataa ccttaatttt
    32581 taaagagcat aaggaaacgt gatatcactt tctcacgaaa ggtgttaggt aggctgtgcc
    32641 taccacgttt ctaagaaaga taaatgatat caaaattttg gatttgtgtg tagatttgtg
    32701 ggtaggatga aagcgaatgt gggcagtaaa ataagcgttt gtgtgtagca aaatcaaaaa
    32761 atgtgtaggg atatgtgtag ctacacatat ccctacacat aataaatttg gaggaaaata
    32821 tggacttatt aattagaaat attgaagaaa agtatattaa taaaattgac aaacgttgtg
    32881 aagaacttac ggtcaagact ggcaaaaaat ggagtcgaaa tcaataccta aaagttttga
    32941 tcgaaaatga ttttgatcac gctcttctga attataaaaa agaccaattt gatcggttgt
    33001 tagaaaaatt tgtggatatt caaacttata atacgaaaac tttagaagaa tatattgcaa
    33061 caaataatca attaattggt ttgttaattg aataataaaa atagtattgt gaatttataa
    33121 aaggaggaaa tcaattttgg caaaagcaac aagaaaacat gtatatcttt acgatatgca
    33181 tatcgaagta cttgatgaaa taatttctac tcatgaagat atatctaatt atagtgaagc
    33241 agtcagacat ttgtgtatga tgtatgatcc aaaaatcgaa gaaaaaataa atccaattca
    33301 aattaaattg aatgcaatgg gaaaagaaat ttctatttta accgaaatgg tttcagagtt
    33361 tgcggatctt catttgaaag atacaggaat actaattggt acaaattcaa ctgtttatca
    33421 agaagcagaa gaacgtgtgg aaggaaaaat taaatccaat caaaccaaaa aatattcttc
    33481 taagttaaag aaaccagtga atgaatctgt tcataccaga gtagatccat ttgatccaac
    33541 gaagattcta aaaagataaa agtagaagga gaacgtgatg aataaatcgc ctgcagttat
    33601 tcttacttgt caatttacct tgcccaataa aaaatctttt tcaacataca ttgattatat
    33661 gacaagagaa aaagcactgg aagaaatgga acgacgtacg cccgaagaag aacaggaact
    33721 gcaacgaatc aagaatgctt tagaggattt ttatattcct gaaggagaaa ctttttcgga
    33781 aagtaagacg gataaagaat tcagtgaaac gtcgaatgaa gctcaatcgt tgatggaaca
    33841 tggaatcaat tttgacaagt tagaggaaca agattttacc aaatatcttt cttatatgac
    33901 ccgtcactat gcgttagaac aaaagaaaga actgacgcaa agggaacaaa aagaatacca
    33961 acggttgaac caagcaatga aaaaatatca aacagagcca cataaaaaag cttcaggaaa
    34021 tcttcctgga gttttttcta tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat
    34081 caaacaaatc ttccaaagag gacaacaaaa aggctcaatc atttatcaag atgttatctc
    34141 tcacgacaat tcgtatcttg aaaaaatggg cttgtacgat cccaaaacag ataccttaga
    34201 tgaagaaggt ttgaaagcag ctggaagaaa aatgatggag acattattcg agaaagaaca
    34261 attgaatgaa acaggttact ggatggcaac gattcaccgt aacactaagc atatccatat
    34321 tcattttgca gttgttgaaa aagaaaatac gcgtaaaccg tatactgaga ttgaaaatgg
    34381 tgtgagttat atcgtaccga aggggaagag aagacaagca acattagacg caatgaaaac
    34441 caattatgta catgagttag aacgtttctc ttatgaaaag gatcgaatga tcggtatgga
    34501 gaaacgaaat ttattgacac gaaaaagtga tttgcgtaac accttaacaa aagtagtaaa
    34561 agaaccagca cgttacgatc gacacgccat gaacttatta aatgaagtat ataaaaccct
    34621 acctgaaaaa cgttcggaat ggaattatgg agatgaaaat cgaaccaagc tttcaccatt
    34681 tacaagggga aaacttgatg agttaaccaa atatgtttta acaaaagaac ctggatatca
    34741 agaatatata cagatttcaa aagcattgaa agaagaagcc aaaagccttt atggggaaag
    34801 caaaagagaa acaaagaatg cagaaaaaaa cgccctcttt gatttaaaaa agcgcacagg
    34861 aaatgcgatt ttaacagaac tgaagagaca ggatagtcaa ataaaaccat taatagaaag
    34921 tgtcaatcag atgcaatcta tttacaatga acgatccttt cgaacactca aacagttgcc
    34981 aacaaatgaa tacaagtata gttatcaaga attcgtaaag cgtaggaaac aattccaaca
    35041 aagagttgtc acgcagcgaa gcttgcgtca attaaatcga acaattaatg cacatgaaga
    35101 aaaataccac gcaagtaaag catatgaaga gcaacagcga agaatccaaa tggaacaaga
    35161 aagacaatag gaggaagaga aatgaaacaa tatttagatc aatggaaagt catagaagga
    35221 tcactaaaag aagaacgaat cgaacaactt cctgattgtt tagaaaaaga gcatttgttt
    35281 cagataagcg aaatgttgag aaacgaacag tttgatccaa atcaattttt agttgttgag
    35341 tacccagcta ctggtgtgta ttgttgtaat catgtgaagg gtgaaaaata ttttattatt
    35401 caagaatacg aaggtaagtt agcgccttat tatactactt gggaaataaa tgaagaggga
    35461 atcaataatt tcccatgtga aagcattgaa gagtctattt ctctcacgga atgttaaata
    35521 aatagagacg taaaagagtc ctgaacgtga ttgtccagga ctctttttca atgaattgtt
    35581 ttccgccaat tcactgagtg gtgcgattca attttattat atcaaaagaa agtgagattc
    35641 caaagtgaag ggaggaagaa gaaatgactc agacaacgat tggtgttgtg gttgaaaaaa
    35701 gcagggataa tctcgttttt gtgacagaaa ttcaaacagg tagagctttt gtagtcacag
    35761 acaaagctgc aaaagcgtat caaaatggca atattttaac gttaaatctg actacgaaga
    35821 tgttcgttga tgcagtagaa aattatccat ttgtttagac aaacgatatt ttacaaggag
    35881 gaaacggaca tggcagaaga aaaagaacca ctagataata gtcgtttggg aaagtccaaa
    35941 agaaaattag tacggttgca aaacgaatta aataatcaat ttcaaaagct atttgatcac
    36001 caaaaattga caaatgggca accaatgaat gacaaacgaa atggtcgttc atggtttaac
    36061 cagcaagaaa aaatagaaaa taaaatacaa agtttgagag aagagatcaa gcaacaagaa
    36121 aaacaggtag aaaaacttga aagacaagaa gagctaaagg aaatgggtta taacaagtac
    36181 ggtggcctgg acatgacgat cgaaaatatt ccaagaatca aagaagaaat cgaacgcttt
    36241 gaaaaaggtg aatcgacttt ttcagctgcc acgatccgaa aataccaacg aaaattggaa
    36301 acattagaac agctgaaaga aaaatcagag aaaggaaaag aaaatcttct tcctgaagta
    36361 caagcgatca ttgacagcgg acgagtgacg caatggaaaa agaatccaac tatctatttt
    36421 ctgaaaggct atcgaaaagt cgctttagaa ctcaatgaaa acgggggatt tgaggaatct
    36481 aaaaaatacc aagccacgtc ggaagaagaa aaggcagctg ttcgtgaatt acttgaagaa
    36541 gctacaccta ctgattctgt gaaggagaaa gaaaccgatc atttctatcg agtagaattc
    36601 aacgaaaatg atccagcaaa agggttgaaa agttatggtg gggagatcgt aacactagaa
    36661 ttgatcgatc aactcaaaac actggataac caatacgccg cagaagcagg ctattataaa
    36721 ttctattttg aagaagtgaa gaatggagaa gtagtcgatc agatacgaat ggatctaggg
    36781 gatggcgtca atagtaatca ccccatctat ttagagttag ctagattgtg cgaacaatca
    36841 cctgaaaagg aacaagaaca aacagtcaaa aatgaaccta tttcaaaagt acaaaacgag
    36901 attaaagaga caaaaacaaa acaaaagggt tatcaaaaac gaacggcaga agagattaag
    36961 caagaagtca aatcactttc ccaacaggcg cttgatgcgg tgaaaaagca tactcattca
    37021 cctgaagacg tcaaagaact tttgaatttt atgtctcgtt ttccagaacg atcctttaga
    37081 aatcaattat tgatcgagga acaatttcca ggcgcaacgg cttgtttagg tcgtgccgcg
    37141 ttgaaaaaag aaggaatcta tatcaaaaaa ggagaacaag ggaacaagat ctttgtcaga
    37201 aaaacggtca aaggatttta tgagaaagga cgtggctttg ttcgagaaac agaggctaca
    37261 ccagaagata aaaaacgaat cgaaagtggt caaatcgaag tgatcaaaaa accttactac
    37321 acgattgaaa aagtgtttga tatcacacaa acacaattga aacctgaaga ctatccgaaa
    37381 atcttcccga accgcgtctt tgattttcaa ttggatcaaa cagggcagac agaacttcaa
    37441 gttgggattc aagcggtagc caaaaatatt ggaattcaaa ttcgagatat gacagaaagt
    37501 gaagtctatc aaagagaatt aggacaagca cgtggggcgt acgttcgtaa cgaatttact
    37561 ggcgaagaag aaatcgtcat gaatactcga aatactccta cgcagcattt agccacaagt
    37621 attcacgaat tagcccatgc tagaatgcac aaattttcag aattagatac tgcaacaaaa
    37681 gaactacagg cagaaatgac ctcctatatt gtgtgcaagc attttgggat ggacacctcg
    37741 gagaaagcta ttccttatat tgcgacatgg acgaagaata gtcagacgtt ggatgataag
    37801 gaagctgcgg aacgtgggaa aatcatgaat gatgtcagtc gtgtcgcaaa tgaattcatt
    37861 caaaccattt ctaatgaaat caatcaatac agagaaagag aaccagagat ccagccagca
    37921 gaagtgaagc aggagcataa aaatactgta gaaacggcag aacaaaagaa tattgtgccc
    37981 gttggctcac tttggatcga caagaaagac gggaaagtcc tggaagaaga cacgggccga
    38041 acgttacatt tgaaggatgc ggcgtttatt gaagatccgc agcataatca agtgatttta
    38101 aaaggaaaaa gttatggaaa agagatccaa acggcgttac cttcagaaga atttgcgcta
    38161 ggaaatccat tccgattttc acttgagaca gtgtcagaag tggcaaaacg aaaaaatgaa
    38221 atctattatg aacaacaatt gtcacaaaaa gaacgcgtat aaaacaagca agaaagacta
    38281 tctgaaaaat ggatagtctt ttttcttttg ggaggtttga aaatgacaga gaaaaaaatg
    38341 agtctcattg atcgatgcaa gcaaatggat attgtggatt ttgcacgaaa caatggttta
    38401 gcggtggcca ataaaggacg agactatcgc ttagaagacc atgattcatt tgtttttgat
    38461 cgaagaaaac aacgttttta ttggaacagt caaaatatta gtggcgacat tattgaatta
    38521 gctaaacttt ttttcataga caaagagatc caagacccta agcaacaatt taaagcagcc
    38581 actgatttta ttctaaagaa tgaagacaaa acagaacgag tggaaaatct tcattttgag
    38641 acagaaaagt acaaagatca ccccgttgat tatcaaccac tcacagaaaa aggaaggaat
    38701 tatctgaagg aagaacgaaa actgcccgat tggttgattg attatgcaga aaaagaagga
    38761 ttgattgccg aactaaagcc aaaacatgaa cggcaaaatt tcttagttcg agacgatcgt
    38821 ttggatcatg cggtcgcctt tttatggaaa gatccacaga caaaagagac agtaggggcg
    38881 agttatcaag gaacgttcat tgattatgaa cgttttggtg agagaggcac atataagcat
    38941 atagataaaa attcgacagc gaatcatggc tttaacctaa agattggtga tcctaaacag
    39001 ttaaaatttt ttgagagtag tattgattta cttagctacg ctgcactgaa tcgtgatcag
    39061 ttaaacgata cgtggttagt ctctatggaa gggttaaaac accatgtgat cagtcattat
    39121 tttggtgagg cagtatctga attgagaaag aaacaagcct ttcctcaatc catcgagatt
    39181 tgtgtggata atgaccgtgc aggacatatt ttctacgaaa aagaacaatt aatgggcgca
    39241 gttgatccat ttacgaatca gaaagtacgt tgtgaacgag gaatcgctaa tgattggcaa
    39301 gtgccaagag aatacaaagt catttatgaa gaagtcgcaa aagaagaaaa ggtcacacca
    39361 gaagctatta tggccataca caagacagaa aacaatctgc aattaacgaa ccaactggtt
    39421 tccgctcaca aagtcaatgc gtcttttggt cagcagcttt ctgttaacga ttcaatagaa
    39481 gtcattagtt tgaaagatat ttgtagagaa gtcgcaaaag aattaaaaga ttgtgaacgc
    39541 tcgaatggta cgtatgattt tgatcgtttt tatcaaggaa aaggggatat taattctcag
    39601 attcttttct cttataaagc agaacaatac tacaaaggct ataaaaatca tgaacatgaa
    39661 tttgtacctg aagtgaaaaa ggattggaac gatcaattga agcatgagat ccatcaacag
    39721 gaaattagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
    39781 tgagatcgtc acgatgaaaa ctgtcatttt agcagaaaaa ccgtcacaag cgaaggccta
    39841 tgccgatagc ttttctaaag ccacccgaaa agacgggtat tttgaaatcc aagatcgatt
    39901 gttttcgggt gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
    39961 cgatatgtac gatgaaaatt ggaaacaatg gtcattagag catttgccta ttttccctac
    40021 acattatcac tatcatgttc ctaaagataa gaaaaagcaa ttcaacgtgg tgaaacagca
    40081 acttcaatcg gccgacacca ttattattgc gacagatagc gatcgtgaag gggaactgat
    40141 cgcttggacg atcattcaac aagcaggcgc tgatcaagga aaaaccttta aaagactttg
    40201 gatcaactcc ttagaaaaag aagccattta tcagggattc cagcaattac gagacgcaga
    40261 agagacctat cccaaatttg aagaagcaca agcacgccag atcgctgatt ggttgatcgg
    40321 catgaacggc agcccactgt atagtttact cttgcagcaa aagggcatac caggaagttt
    40381 ttccttaggc agagtccaaa ccccaacgct ttacatgatt tatcagctgc aggaaaagat
    40441 tagaaacttc caaaaagagc cttattttga agggaaagcc caagtaaccg ctcaaaatgg
    40501 catatttgat gcgaagcttg atcccaatga aacccaagcg acgcaagaag catttgaagc
    40561 ctatttaaaa gaaaaaggtg ttcaagtagg aaagcagcca ggcacgattc ttgaagtcga
    40621 aaccgagaaa aaaagcgcgg caagtccacg tcttttttct ctatccagcc ttcagtctaa
    40681 aatgaatcag ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
    40741 tgaagggaaa tatctaagct atccacgaac agacacccct tatattacag aaggagagca
    40801 tgcgtatctg ttggatcatt tagatgaata caagcatttt ttaaaggctg aatcattccc
    40861 tactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
    40921 cgcgatcatt cctacaaaaa cagttatgac agaagcaacg tatgaaaagc tgacaccttt
    40981 acaacaagcg atttatgatc aagttttaaa gacgacggtg gccatgtttg cggaaaaata
    41041 tacctatgaa gaaactacga ttttgacaca agtccagcag cttcagctaa aggcagtcgg
    41101 aaaagtacca gtagatctag gttggaaaaa gctatttggc aaagagagcg acagaaaaga
    41161 gaaggaagaa gaaccgctac ttcctaaagt aacaaaagga gaaacggtga cggtagatct
    41221 acaaatcttt gaaaaagaaa ccaagccacc acaaccttac acggaaggta cactaatcac
    41281 cgccatgaag acagctggta aaaccgtgga tagtgaagaa gcacaatcca tattgaaaga
    41341 agtggaggga attggtacag aagcgacacg agcaaatatt attgaaacct tgaagcaaaa
    41401 ggaatatatc aaggtagaga aaaataaact tgtggtgacg aataaaggaa ttttgctttg
    41461 tcaggctgtt gaaaaagaac cgctgttaac aagtgccgaa atgaccgcca aatgggaaag
    41521 ctatcttaca aagattggtg agcaaaaagg gacacaagct acttttctag caaatatcca
    41581 aaaatttgtt tcccacttgt tggaagtcgt cccaggacaa atccaagcca ctgattttgg
    41641 agcaacgtta caagaggtga aagtagccag tgaaaagcaa gaagcggcgc gtcatttagg
    41701 atcttgtcca aagtgccagg aaaaagaagt catactttat cagaaggtgg cgacttgcac
    41761 gtctgaagcc tgtgatttta aattgtgggt aacgattgcc aaaaagaaac tcacagcgac
    41821 acaactgaaa gagatcattc agaacggccg aacaagtcaa ccagtcagag gactaaaagg
    41881 tcagaaaggt tcgtttgaag caacgatcgt tttaaaagaa gactttacaa caggatttga
    41941 gttttctgaa aagaagaaga ccacctacaa aagaaaagtg agacgtaaaa cgagttagaa
    42001 ttgacggagg gaaaaacgat gagaaataca acgcataaag aacaagaaat cgaaaatcaa
    42061 aaagccaaaa tacgttcaga gcaggagaaa atgcgtgcca gcaaacgaag actacagcaa
    42121 ttgaagaatc aacaacggat tcaaaaagaa cttgaattag atcgataaat cctcctgttg
    42181 ttaatattaa actaacatgt tatagttatg ttagtttaat attaacattc gagagggaaa
    42241 gcgatgaaag ataaagtcaa gtgcattttt gagaaataca atggtaatct atccttgaag
    42301 gatgctagaa atgaagggtt atcacctatg acgttagatc gtttagccga aaaggacaaa
    42361 atagaaaaaa tagcatcagg tttttatacc ttaaaagata gttttgtgga tgaattatat
    42421 ctcgctcaaa gtatctatac tagaggaatc ttctctcacg agacagcgct tgatcttttt
    42481 caagtaggaa catatatccc taaaaaaatt aatatgatgt ttcctaaagg atacaatgtt
    42541 tcgaaagaga atttagatca gtatgccatt caaccgcatt acacgaataa agaaaacttt
    42601 gaactgggaa tcacagaggc agaaagcttt tacggcaata aaattttcgt ctatgattta
    42661 gagcgaacgt tatgtgatat gtggaatccc agatataaag caagcgtaga agtaaaacaa
    42721 gaagcattaa aagaatatat gacaagtacc agcagaaata caaataagct aagaagatac
    42781 atgaatattt taaaatcacc aaaagaaatg actttgtata tgttgccact ttattgagag
    42841 gagtaaaggt gaatgacacc gacacaattt aaagcgaaaa caagaaatgt tgcagctgag
    42901 aaacatttaa acccacaaat tgttcaaaga tattatatga tggaaaaatt attagaatgt
    42961 attgttgaat cgccttatca tgatgatttt gtattaaaag ggggatttct gatcggatca
    43021 aaatacgggt tagaaaaccg ttcaaccgtt gatattgata cgacttatcg aaattcgaaa
    43081 ctaacaaaag aaaaattaga aactgttttt tctgaattaa cagctgctcc tacaaaagat
    43141 ggcattcggt ttgaattaaa gggcttaaca gagacgaggg aagccgatta ttacccaggt
    43201 tttcaagcaa agtttattgc ttttcttgaa aatgcacgtg taccttttaa attagatatt
    43261 acttcaggtg attctattat tccagcagca ttgatatatg aacataaatt gatgtttgaa
    43321 gataaaacaa ttcatattcc agcttatcct acggaacaaa ttttagctga aaaattacat
    43381 gccacgttta gtttcggaat agataattca cgtatgaaag atttctatga tctgtatatg
    43441 atccctaaac tagaacgtat cgatgagata aatttgtata agagtgttca aggaacattt
    43501 aaggaaagaa ataatcaatc agtgtttaag aatttgtata atcaggaaat gcctcaattg
    43561 atgaagaatg agggattgag ggagaaatgg aaaagatatc agcaagaaaa ctattttgcc
    43621 aaagatattt ctttcgaatc taccttaact tctgttgatt cgctgatgca atcaattgtc
    43681 gagcaagagg aagtagaacg aaaacaaaat tttaagagat cacaacaaat gaatcaagaa
    43741 atggagagat aaagatgatc aataatgtaa ctttagtagg tcgattaacc aaagatcccg
    43801 atttacgtta caccgcaagt ggcactgcag tgaccacttt taccttagcc gttaaccgta
    43861 attttactaa tcaaagtggc aatcgagaag ctgactttat caattgtgtg atttggcgca
    43921 aaccagctga aacgatagcg acattagcta aaaaaggcag cttgatcggc gtcgtcggac
    43981 ggattcaaac gcggacttat gacaaccagc aaggacagcg tgtctatgtg actgaagttg
    44041 tggccgataa tttccaacta ttagaaagta aagcagcgac agaaagccgt atgcacgctg
    44101 atcaatcgtc cagcagtcca agccctacca cctttgaaca acgggataca gcgacgccta
    44161 acaacaatgg attgaacgct tcacagaatc catttggtgg acaatcaatc gatattagcg
    44221 atgatgatct accgttctaa gtagcatttt ctattggttg catttgctac ggtttatgtt
    44281 ataatttaga caacaaaaag aattttcaat ctataaccaa aaaccactag ctggcgggct
    44341 agtggctttc ttttacgttt ctattataag aattaacggg taggcttact gtctttcttg
    44401 gcaaatttac aatcaagcca tttgcagata tagtgcgcgg ctacttctgc catgattgca
    44461 aaaacaaaat caaaaagaat tttcaaacct ataacctcct ccccttgcca gattaggccg
    44521 gggtagcaga cagcaagact attatagcat aattgaaata tccaaatgat tggatttttg
    44581 ctataatttt agcgaggtga ttgtgatgaa caaatatgtt ggtttgcttg ataaaatccg
    44641 agtaataaaa acgcaaccgt tattggttcg ctttacttta caaacgattc atgaatcgat
    44701 taattgtgta gttgcagata ttgaaattat cgataaattg ctaattatgg atgatggaaa
    44761 atacaatatt gccgtcacag gtcacttcaa taaacgaaat cagctagtta ttgctagcat
    44821 gtatgttaga aatcccgatc attttacgcg atcgatgggc atataaataa aagaagagtt
    44881 gtagtcctaa atgaggattg cagctctttt tgttttacat agaaacagga aaagaccttt
    44941 ttaggaagac acaagtgcct tctcaattga aaaacctttc tctcactcca accgccaccg
    45001 ccgttcgaga agaaaggaag tgaacgctgc acgttcacta agatatggtg tcttgcaaag
    45061 caagacaaaa tagacaaaac ttcctctcac caaaaggtt
//
LOCUS       DACDRK010000013        43825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDRK010000013 DACDRK010000000
VERSION     DACDRK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.013"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(172..516)
                     /locus_tag="IWJ77_000834"
     CDS             complement(172..516)
                     /locus_tag="IWJ77_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9452474.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(509..943)
                     /locus_tag="IWJ77_000835"
     CDS             complement(509..943)
                     /locus_tag="IWJ77_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9452475.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1081..1818
                     /locus_tag="IWJ77_000836"
     CDS             1081..1818
                     /locus_tag="IWJ77_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452476.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1811..3022
                     /locus_tag="IWJ77_000837"
     CDS             1811..3022
                     /locus_tag="IWJ77_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452477.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3229..4008
                     /locus_tag="IWJ77_000838"
     CDS             3229..4008
                     /locus_tag="IWJ77_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9452478.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4078..4740
                     /gene="trmB"
                     /locus_tag="IWJ77_000839"
     CDS             4078..4740
                     /gene="trmB"
                     /locus_tag="IWJ77_000839"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9452479.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4838..6592)
                     /gene="ade"
                     /locus_tag="IWJ77_000840"
     CDS             complement(4838..6592)
                     /gene="ade"
                     /locus_tag="IWJ77_000840"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9452480.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6724..7653
                     /gene="cpaF"
                     /locus_tag="IWJ77_000841"
     CDS             6724..7653
                     /gene="cpaF"
                     /locus_tag="IWJ77_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9452481.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7610..8659
                     /locus_tag="IWJ77_000842"
     CDS             7610..8659
                     /locus_tag="IWJ77_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9452482.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8656..8934
                     /locus_tag="IWJ77_000843"
     CDS             8656..8934
                     /locus_tag="IWJ77_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9452483.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8909..9379
                     /locus_tag="IWJ77_000844"
     CDS             8909..9379
                     /locus_tag="IWJ77_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9452484.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9396..9698
                     /locus_tag="IWJ77_000845"
     CDS             9396..9698
                     /locus_tag="IWJ77_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452485.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9715..10086
                     /locus_tag="IWJ77_000846"
     CDS             9715..10086
                     /locus_tag="IWJ77_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9452486.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            10058..10417
                     /locus_tag="IWJ77_000847"
     CDS             10058..10417
                     /locus_tag="IWJ77_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452487.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10589..11596
                     /locus_tag="IWJ77_000848"
     CDS             10589..11596
                     /locus_tag="IWJ77_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9452488.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11621..12805
                     /locus_tag="IWJ77_000849"
     CDS             11621..12805
                     /locus_tag="IWJ77_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9452489.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13242..14825)
                     /locus_tag="IWJ77_000850"
     CDS             complement(13242..14825)
                     /locus_tag="IWJ77_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9452490.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15403..15546)
                     /locus_tag="IWJ77_000851"
     CDS             complement(15403..15546)
                     /locus_tag="IWJ77_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9452491.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15768..16247)
                     /locus_tag="IWJ77_000852"
     CDS             complement(15768..16247)
                     /locus_tag="IWJ77_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9452492.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16320..16604)
                     /locus_tag="IWJ77_000853"
     CDS             complement(16320..16604)
                     /locus_tag="IWJ77_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452493.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16682..16876)
                     /gene="ssrS"
                     /locus_tag="IWJ77_000854"
     ncRNA           complement(16682..16876)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWJ77_000854"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16948..18234)
                     /locus_tag="IWJ77_000855"
     CDS             complement(16948..18234)
                     /locus_tag="IWJ77_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9452494.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18372..19043
                     /locus_tag="IWJ77_000856"
     CDS             18372..19043
                     /locus_tag="IWJ77_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9452495.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            19009..19497
                     /locus_tag="IWJ77_000857"
     CDS             19009..19497
                     /locus_tag="IWJ77_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9452496.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19510..20457
                     /gene="prmA"
                     /locus_tag="IWJ77_000858"
     CDS             19510..20457
                     /gene="prmA"
                     /locus_tag="IWJ77_000858"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9452497.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20458..21222
                     /locus_tag="IWJ77_000859"
     CDS             20458..21222
                     /locus_tag="IWJ77_000859"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9452498.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21455..23668
                     /locus_tag="IWJ77_000860"
     CDS             21455..23668
                     /locus_tag="IWJ77_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9452499.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23679..24125
                     /locus_tag="IWJ77_000861"
     CDS             23679..24125
                     /locus_tag="IWJ77_000861"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9452500.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24296..25555
                     /locus_tag="IWJ77_000862"
     CDS             24296..25555
                     /locus_tag="IWJ77_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9452501.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25644..26591)
                     /locus_tag="IWJ77_000863"
     CDS             complement(25644..26591)
                     /locus_tag="IWJ77_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9452502.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26985..28277
                     /locus_tag="IWJ77_000864"
     CDS             26985..28277
                     /locus_tag="IWJ77_000864"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9452503.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28292..30061
                     /gene="aspS"
                     /locus_tag="IWJ77_000865"
     CDS             28292..30061
                     /gene="aspS"
                     /locus_tag="IWJ77_000865"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9452504.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30170..31045
                     /locus_tag="IWJ77_000866"
     CDS             30170..31045
                     /locus_tag="IWJ77_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9452505.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31311..33194
                     /locus_tag="IWJ77_000867"
     CDS             31311..33194
                     /locus_tag="IWJ77_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9452506.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33269..33553)
                     /locus_tag="IWJ77_000868"
     CDS             complement(33269..33553)
                     /locus_tag="IWJ77_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9452507.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33543..34277)
                     /locus_tag="IWJ77_000869"
     CDS             complement(33543..34277)
                     /locus_tag="IWJ77_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9452508.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34406..35029
                     /locus_tag="IWJ77_000870"
     CDS             34406..35029
                     /locus_tag="IWJ77_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9452509.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35221..36342
                     /locus_tag="IWJ77_000871"
     CDS             35221..36342
                     /locus_tag="IWJ77_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9452510.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36349..39477
                     /locus_tag="IWJ77_000872"
     CDS             36349..39477
                     /locus_tag="IWJ77_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9452511.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39496..42690
                     /locus_tag="IWJ77_000873"
     CDS             39496..42690
                     /locus_tag="IWJ77_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9452512.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42836..43543)
                     /locus_tag="IWJ77_000874"
     CDS             complement(42836..43543)
                     /locus_tag="IWJ77_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9452513.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14138 a         7780 c         9240 g        12667 t
ORIGIN      
        1 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
       61 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
      121 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
      181 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
      241 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
      301 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
      361 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
      421 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
      481 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
      541 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
      601 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
      661 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
      721 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
      781 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
      841 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
      901 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
      961 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     1021 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     1081 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     1141 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     1201 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     1261 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     1321 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     1381 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     1441 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     1501 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     1561 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     1621 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     1681 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     1741 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     1801 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     1861 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     1921 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     1981 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     2041 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     2101 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     2161 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     2221 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     2281 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     2341 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     2401 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     2461 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     2521 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     2581 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     2641 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     2701 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     2761 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     2821 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     2881 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     2941 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     3001 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     3061 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     3121 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     3181 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     3241 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     3301 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     3361 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     3421 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     3481 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     3541 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     3601 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     3661 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     3721 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     3781 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     3841 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     3901 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     3961 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     4021 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     4081 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     4141 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     4201 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     4261 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     4321 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     4381 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     4441 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     4501 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     4561 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     4621 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     4681 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     4741 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatctca gaaaatgaac
     4801 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     4861 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     4921 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     4981 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     5041 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     5101 gctgtttgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     5161 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     5221 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     5281 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     5341 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     5401 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     5461 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     5521 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     5581 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     5641 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     5701 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     5761 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     5821 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     5881 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     5941 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     6001 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     6061 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     6121 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     6181 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     6241 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     6301 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     6361 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     6421 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     6481 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     6541 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     6601 ctcccaagat aaatttttat aagtataaca taaattcggc ttttgccaat atttttttgt
     6661 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     6721 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     6781 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     6841 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     6901 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     6961 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     7021 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     7081 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     7141 agcgggacag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     7201 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     7261 cagatcaatc cgaaaatagc acagacttat gatacacttt tgaaactcgc attacggcat
     7321 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     7381 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     7441 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     7501 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     7561 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     7621 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     7681 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     7741 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     7801 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     7861 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     7921 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     7981 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     8041 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     8101 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     8161 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     8221 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     8281 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     8341 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     8401 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     8461 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     8521 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     8581 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     8641 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     8701 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     8761 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     8821 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     8881 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     8941 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
     9001 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
     9061 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
     9121 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
     9181 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
     9241 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
     9301 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
     9361 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
     9421 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
     9481 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
     9541 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
     9601 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
     9661 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
     9721 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
     9781 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
     9841 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
     9901 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
     9961 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    10021 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    10081 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    10141 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    10201 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    10261 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    10321 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    10381 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    10441 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    10501 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    10561 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    10621 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    10681 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    10741 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    10801 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    10861 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    10921 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    10981 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    11041 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    11101 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    11161 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    11221 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    11281 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    11341 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    11401 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    11461 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    11521 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    11581 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    11641 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    11701 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    11761 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    11821 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    11881 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    11941 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    12001 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    12061 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    12121 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    12181 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    12241 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    12301 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    12361 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    12421 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    12481 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    12541 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    12601 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    12661 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    12721 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    12781 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    12841 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    12901 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    12961 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    13021 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    13081 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    13141 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    13201 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    13261 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    13321 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    13381 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    13441 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    13501 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    13561 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    13621 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    13681 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    13741 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    13801 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    13861 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    13921 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    13981 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    14041 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    14101 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    14161 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    14221 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    14281 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    14341 cctccgctaa gttctgttaa ccgtgcttcc tgacagtgtg gaatccctaa gccttgcaag
    14401 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    14461 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    14521 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    14581 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    14641 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    14701 gatttgccag ttccgttgat atcgactaag gcaatttttt caccagcttc aatttgacag
    14761 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    14821 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccctg gcctgtgtta
    14881 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    14941 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    15001 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    15061 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    15121 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    15181 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    15241 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    15301 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    15361 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    15421 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    15481 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    15541 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    15601 cgactaatcg ccctggtgtt cacttttttt attttacttg aactcattga ctgttttccc
    15661 tttattttaa acattataaa gaccgatgag tgcttgccgc gtcttttgtt tttatcacgg
    15721 ttttttgtgc aaaaaaacct gccgatagtt ggcaggtcgt ctacgtgtta agctttggtg
    15781 tggtctttag ctggcaattg gttttctaaa tcagttcgga caacaagcac atcgcaagct
    15841 gcgtttctaa taacatattc agaaacagaa ccaataaata aacgttctac agcattcaat
    15901 cctgtggctc ctaacataat caaatctact tgattatctt ctgggatttc tctggcaata
    15961 atttgttttg gtgagccata ttctacgaca gaggttacat tattcaagcc agctttcttc
    16021 gcattgcttt cataatctgc caatgtttgc tttgccattt cagtagcttg ttctgcaagc
    16081 attccatcaa aggatgaaac ggtttggaaa gaacgtgtgt caatgacatg tgctaataga
    16141 agttctccgt tatttcggat agctacattg accgcctttt taaaagctaa ttcagcttca
    16201 tcagaaccat ctacggcaac catgattttt ttgtattgtt gtaacataag cattcactcc
    16261 tcaagtcaat cgtattgtac tcttttattg tagaacactt tagggaaaaa gagaattatt
    16321 tactgctttg gttcagcaat tttttttgca aatcggatac tgaaggtggc agaaacaata
    16381 attacgatca gtaaaaagac caaggcaata aagcgttgat tgaaaaaagc tgttaaaata
    16441 cccattacgc ctaaaacagc atatgcagca ccgtaaaatt gtaggaatcc ttgattttta
    16501 tcatttttgt tgatcaatac aaaaaagata tcctgtttat taaataaata atagctaacg
    16561 aataataata ctaaagtggc gataatcatt aaaatcctaa tcatgaacaa atcctccaaa
    16621 ataagcaaca aatagaaaaa gcaatttgcc aagagcaaat tgctttattc tttaaaagat
    16681 ggtaatctcc gttaatgccg ttgtttgtcc tgtagtattg atcccgcgaa ttcaagcagg
    16741 tgggtcccat ggtcgtagct tcgaactacc aaatgataag gcatgttacc agcagtgaca
    16801 caagacgggt cccaagatat caataaaaat gttcggtcaa cacacaaaga ggacaattcg
    16861 aacatcacag atttcccacc tctatagtag catatgaatt taaaaagagc aatgagagag
    16921 ctcgcagaaa cagaaccgtt ttcttgtcta gttttttgga ggatgctttt tccactcttt
    16981 tatacggtaa tactgttgac ccagagcctg ttcatatttc cctgtatctt ttggctgata
    17041 atactgagca ttcttcagtt tatctggtaa atattgctga ttgacccaag cctgatcaaa
    17101 atgatgagga tactgataac ccacgccacg gttgagtgat ttcgcacctt tgtaatggct
    17161 atctctcaaa tgatcaggaa catctccagc tttgccctct cgaatatcag caagtgctgc
    17221 atctaatgcc atgtaggcag aattggattt tggcgacaag caaagatcga cgacgacgtc
    17281 tgctagtggg atacgtgcct caggcaatcc caatttttca gcagctaata cagcgttcac
    17341 tgtacgagcc gcagcagcag gattacctaa acctatatct tcataaccga taaccatcag
    17401 acgccggcaa atacttgcca gatcacctgc ctcaactaaa cgggcgagat aatgaagtgc
    17461 agcatccaca tcacttccac gaatcgattt ttgaaaagcc gaaataacgt cataatgagc
    17521 atctccattt ttgtcatggg taagagcttt tctttggaca cattcttcaa tgatcgacag
    17581 tgtaagatga atccttcctt ctttatctga aggagtcgat agtgtagcca gttccagccc
    17641 attcaatgca ctccgcaaat cgccgttcgt tgcacgcgaa aggtgaagaa gagcatcttc
    17701 gtctaactgg attgcttgtt gtccaaggcc gcgttcttta tcccttaaag catgttcgac
    17761 tgccaactgg atgtcttgtt ctgttaatgg tttgacttca aagatctgtg tacggctgcg
    17821 gatcgctgga ttgatggtaa tataaggatt ctcagtagtc gctccgatca agatgattcg
    17881 gccactttct aaatgaggaa gcagaaaatc ctgttttgtt ttatctaatc ggtgtacttc
    17941 atccaataag aggatcactg taccactcat ttttgcttct tccgctacaa cttgtagatc
    18001 cttttttgaa tctgtagcgg cgtttagcat gcgaaatgca taattagtag agccggcgat
    18061 cgcacttgcg atactcgttt ttccagtacc aggaggtcca tatagaatca tggaagagag
    18121 catgcgagca tcgaccatgc ggcgtatgat ttttccttcc ccgactagat gctgctgacc
    18181 aacgacttca tcgatcgttc gaggtctcat gcgataagcc aatggctgtt gcataggatt
    18241 ttcctccttc ttgtttttac gtcccttaat tatatcatat ttagcaagat gcctagaaag
    18301 aacagccttc tgttgttttg ttttcatagc ttatccaact aatattgtgt atgataaagt
    18361 aggtgagaaa aatgacggat aaacaaaaga tagatgaatt tttttggaat aaaacgacgc
    18421 cagaagtggc acgggattta ttggggatgt atttggagca tgacacacca gaaggaagat
    18481 tagctggata tattgtagac gcggaagcat acctaggtcc agaagacgag gccgcccata
    18541 gctatggttt aagaagaaca ccgcgtgtgc gggcaatgta tgaaaaacca ggtacgatct
    18601 acctttacac gatgcatact catcggattt tgaacatcat tactcagcca gaagggattc
    18661 ctcaaggagt aatgatacgt gcaatcgaac ccgccgcaat gattgatcaa atgagtaaaa
    18721 atcgtggcgg aaaaactggt ccagatatca gcaacggtcc gggaaaatta gtggaagcat
    18781 tagcaattcc tcaagaactt tatggtcagt ctatagcaga tagttcgtta cgcttagttt
    18841 ttgagaaaaa aaagacacca aaaaagatca atgctttgcc tagaatcggt atcccaaaca
    18901 aaggtgtatg gacagaaaag ccgttgcgtt ttgttgtgtc gggaaatcct tatatctctt
    18961 tacaaagaaa aaaccaaatc gagaaagatt ggggttggag gaaagaaaat gaaaaagaag
    19021 gatctattaa catatttaga tgaaataatt gaaaaacaag tgacagacta tgatgtagct
    19081 ttagattggg atacaaaaaa tcatacgatc gagatcgttg ttcgcttgtt tgcagaaaat
    19141 aaagcacaat ttgagattga tgatgcagaa gggatcgttt cgcaagaacc aattatcgaa
    19201 tttgaagacg gcgtacttct ttttaatcct cagaaatctg tttttgatga acgcaattat
    19261 ctagcagtaa tcccttatga aggaaaaaaa gggttggcaa aatcagtagc tgattcttta
    19321 gtggaatatc taaatgaagt attagctcaa gggcaaagcg atttgcttga ttttttggat
    19381 gaagacgatg acgaagctgt attcgagctt cactgggata atcaacaact agcagaatta
    19441 gtagaagtaa aacagcaaaa tgaagcagat acttatttac cgtacccaag ttattaagga
    19501 gtgacagata tgaaatggaa tgaagtaaaa gtagaaactg aaagtgaagc agtcgaagca
    19561 gttgctaata ttttgatgga ggcaggggcg agtggtgtcg cgattgaaga ttctttagat
    19621 gttgaacatt ttcagtcgga tcaatatggt gagattttag ataaaaatga ttttacatcg
    19681 ctgaaagaag gcgcactagt catggcttat tttccggaaa cgactttctt accggaaatc
    19741 ttgcctttta tcaaagatcg tatcacacaa ttacctgagt ttggtctagc tattggaaaa
    19801 aataaagtaa cagttagtga agtagaggaa agcaactggg caacagcctg gaagaaatac
    19861 tatcatccgg tgcgtattac gcgaatgctg acggttgcgc caagctggga aaactaccag
    19921 acagaagatc ctctagaaaa aattatcaca ctagatcctg gtatggcatt tggcacaggc
    19981 actcatccca cgacttcctt gacattgaca gcactagaaa cagtcttgcg tggtggtgaa
    20041 acattgctag atgtgggaac tgggtcgggc gtgctaagta ttgcagcaaa atatcttggt
    20101 gcaaaagatg tctatgcctt tgatttagac gaagtagccg ttcgttcggc gaaagaaaat
    20161 atggatatga atgaaatagc caaagatgtc catgtatcag ctaacgatct tttgacagga
    20221 atcgagattg aaagtgatgt aatcgttgcg aacattttag cagatatcat cttattgatg
    20281 atcccagatg cttggcgttt actgaagcaa acaggcacgc tgatcgtttc aggaatcatt
    20341 gaagagaaga agcaaatggt tctggatgcc atgttagaac aagggtttat cgtggatcaa
    20401 attttccagc aaaaagactg gtatgcaatt attttgaaaa aaccagaggt ggaatagatg
    20461 cagcggtatt ttttaaaaga atcatatgaa tcaaaagact attttgtttt agaaggggaa
    20521 aactatcacc acattgtacg ggttatgagg atggaacccg aggatgaagt ttttttggca
    20581 ttcaatgatc aaatagccat tgttgcagaa atcacggaga ttactgaaga ggctgtttat
    20641 ctgaaagaaa ttaaaaaaga agaaaatcaa aaggaactgc caagttcgat taccatagcg
    20701 agtggctatc ctaaagggga aaagctggat tggatcgttc agaaatccac tgaattagga
    20761 gcgcaagctt ttcttggttt cccggcatca gcctctgtcg ttaaatggga tgcaaaaaaa
    20821 cgtatgaaac gacaacaacg gcttgaaaag attgctcaag aggcagcgga acaatcccat
    20881 cggcagatcg tgccatcagt agagttattg gaaacgaaac aagatttatt tgagcggcta
    20941 gatgattatg acgtggtctt ggttgcttat gaggaatcag caaagcaagg cgaaaaatca
    21001 aatgcagcca aaattcttag tgaaacaaaa ccagatagtc gaatcttagc tgtctttgga
    21061 ccagaaggtg gattatcccg cgatgaagtc gattattttg tagaacacaa agggatactt
    21121 tgtggattag gaccaagaat ccttcgtaca gaaacagcac ctctttattt attaagtgct
    21181 gctagctatc aatgggaatt aatgaatcct tctttcagct aacgttcttc attaataaaa
    21241 gagtatcgac agaaagaatt gaaaaaatag tcaacaattt gtataataga aacaattatt
    21301 tatacaatgg atacgaaaaa tcgtttgaaa aagcttttaa aaagcgaaca tttccgtaaa
    21361 aaaacgtata actgcacgga ttttgggaca attatcaaac agatatttgt cccgattccc
    21421 gttttaccga aaaaagaaag ggggatcgat tcctgtgaca aaagaagaaa ttttaacggg
    21481 acccggtgtc ataaagctcg ttagtcaata catgggtccc gaacatgtag catttgttga
    21541 gaaagcatgc gaatacgcga ctgctgcaca tgacggacag tttagaaaat caggcgaacc
    21601 ctacattatt catcctatcc aagtcgcagg tatattagca gatttaaaaa tggatcccca
    21661 tacagtggct acaggcttct tacatgatgt tgttgaagat acagaaatca ctttagaaga
    21721 tctgagagaa gaatttggcg atgacgttgc tatgttagtg gacggcgtaa ccaaattagg
    21781 gaaaataaaa tataaatccc acgaagagca gctagcagaa aaccaccgaa agatgctgct
    21841 agcaatggct caagatttac gagttatcat ggtcaaatta gctgatagac tacataacat
    21901 gcgtacgttg aagcacctgc gagaagataa gcaaagaagg atcgctcagg aaactttaga
    21961 aatctatgcg cctcttgcac atcgtctagg gatcagccgg atcaaatggg aattagaaga
    22021 tacagcactt cgttatctaa atccaaaaca gtattaccgt atcgtccact tgatgcagac
    22081 gaagagagaa gaacgtgaaa aatacgtaag tggtactgtt gaagatattc gaatagcgac
    22141 agaagagctg gggatttttg cagaaatcta tggacggcca aaacacattt attcaatcta
    22201 tcgtaaaatg aaagatcaga aaaagcagtt caacgaaatt tatgacttgc tagctattcg
    22261 agtgatcgta gattcaatca aggattgtta tgctgtatta ggagcaatcc acacaaaatg
    22321 gaaaccaatg cccggcagat ttaaggatta tattgccatg ccaaaagcta atatgtacca
    22381 atctttgcat actaccgtga tcggtccggc aggaaatccg gtagaaattc aaatccgaac
    22441 acaggaaatg catgaaatcg ctgaattcgg ggttgctgca cactgggcct ataaagaagg
    22501 aaaaaatgaa aaagtagaac cagatggtat gacgaaacaa ttaagctggt tccatgagat
    22561 actcgaactt caagacgaaa gctatgatgc ttctgaattt atggaaggcg taaaaggaga
    22621 tatctttagt gataaagtct acgtcttcac accaaaagga gacgttactg agttaccaaa
    22681 aggatccgga ccattggact ttgcatacag tatccataca gatatcggta acaaaaccac
    22741 tggtgcaaaa gtaaatggca aaatggtgca gcttgattac aaattgaaaa acggagatat
    22801 cattgagatc atgacttctc caaattcatt tggcccaagt cgcgactggt tgaaattagt
    22861 tgctactagc aaagcaagaa ataagatcaa acgtttcttc aaagcccaag atcgagaaga
    22921 aaatgtgatc aaaggccacg aatccgtggt caaatgtatt acagatctag gatttacgcc
    22981 taaagatatt ttgacgaaga acaaactgca agaagcactc gatcgtttta attatcaaac
    23041 agaagatgat ctctatgcag ctgtagggta tggagaagtt agccccttga cgatggccaa
    23101 tcgtctgact gaaaaagaac gtaaagaaca aaaaatcgag cagcaaaagc aagaagcaga
    23161 agaaatcatg aatcagccga aaaaagaacc tgacaaaatg aaagtacgtc atgaaggtgg
    23221 cgttgtcatt caaggggtag aaaacttact aattcgtatc agtcgctgct gtaatccgat
    23281 tcctggtgat gatatcgttg gttatatcac taaaggcaga gggatatcca ttcatcgtcg
    23341 agattgtccg aatgttcagc ctgacaaacc aaatgtagca gaacgtttga ttgaagtcga
    23401 atgggaagat acatcgaata cacgaaaaga gtatgatgca gatttggaaa tttacggcta
    23461 taatcgttca ggcttattga atgatgtact tcaaacagtc aatgcgctaa cgaaaaatct
    23521 caacagcgtt gaagcacgga cgaataaaga taaaatggcg acgatccatt tgacggttgg
    23581 tatccagaat ttatcccacc taaagagtat cgtggataaa atcaaagcag tacctgatgt
    23641 ctacagtgta cgccggacga atggatagga ggacagatat gagagcagtt gttcaacggg
    23701 taagtaaagc tgctgtgtca gtcgatcagc aagaaattgg aaaaattggc tacggcctac
    23761 ttgttttatt aggcattcat gaaaccgata cgcaaaaaga tgtggactat ctggtaaaaa
    23821 aaattgcaca aatgcgtatt tttgaagatg aacaaggaaa aatgaatatt agtgtagaag
    23881 atgtgggagg acaaatttta agtatctctc aattcacact gtttgcggat acgaaaaaag
    23941 gaaatcgacc aagctttgtg gaagccgcac gaccagaaac agctattcct ttatacgaat
    24001 cattcaatga aggaatcaga agtcgcggca tcacagtcgc taccggacaa ttcggagcag
    24061 atatgtcggt ttctctcgtc aatgatggac ctgtaacgat cattattgat tctaaaaaca
    24121 aataaagtaa tctggttttc actagtaaaa actatgaaac acgtcgctta agttagactg
    24181 ataaactcta acttaagtgg cttttttgtc tgcaaaaagc gcttcttttg tcagcagtcg
    24241 gaaaaaaagt taagcttaaa ttatacaagt tattttttat aggaggttgc tgaagatgaa
    24301 agaagtacca gtagtagata tcaaaatcac agatttaaaa aaattgtatc aaaaaggata
    24361 caacatgttg gaggagttac gccatgaagc ggacgctccc gtcgtcaaag caaagatttt
    24421 caataaagaa gctatcacga tctacggatc atcagcagca aaagtatttt atgatccaag
    24481 gaatttcaaa cgaaaaggtg ctatgccaaa attagtattg aagacacttt ttggtcaagg
    24541 tggagtacag actttggatg gagctgctca tcaccatcgg aaaaatattt ttatggatct
    24601 aatgacaccg gaaagaatgg aagattatca tcgtatctta gataagaatc tgacacaagc
    24661 acttgaagca cagcatggtc aatttgaact atttgattta agtaagatgg ttttctttac
    24721 atctatttgc gagtgggcag gtatcaattt atcagcgatt tctaaagatg aggtcgaaaa
    24781 attagcagaa tatcaaatct ctatgatcag cggaacgttt acttctccga ttgatcacat
    24841 aaaaggtgtc gagaatcgca aaaaatcaga aaaatgggcg caaggcttga ttgaagaagc
    24901 tcgacaaaat cctgtggctg gaaaagaaaa tgtggcatta tatgcttttg ccaatgccac
    24961 agatttagat ggtcagcttt tacctctgga agtggcagct gttgaattat tgaacatcat
    25021 ccgtccaacg gttgctctaa ctgtgtgggc ggcattgatg ggacatgcac ttttcagtag
    25081 accagatttg tatcagcaat taaaaaatga cttttcgacg ttacaagatc cgtttattca
    25141 agaaatgaga cgctattatc ctttcttccc aatgctacct gcgatttcgc tgaaagaagt
    25201 agaagtagat ggttatcgta taccagaagg cagttgggtt attttagatt tatatggaac
    25261 agaccatgat gaacgaacag ttgaagcgcc agattccttt atgatcaaac ggtatgtagg
    25321 gaaagcaaaa gatatttcct ataaagaaga atatgagatg attgcacaag gcgggggaaa
    25381 ttttcgacaa atgcatcgct gtgcaggaga atggatcact cttcatagtc tacgtgtgtt
    25441 ttccgatcag ctagtaaata agtttgagtt ctctgtacct gaacaagatt ggacgattcc
    25501 attcaatcag tttccgactt atccgaatag ccgagcgcta ctttacaaga attagattag
    25561 cataaactta tcgaaaaaaa ggaagatcaa acagtgctga tcttcctttt ttcatattac
    25621 tataaattga taaggtcaat tgatcaaaat ccacgaaatg cttcatcaat ggtttgataa
    25681 tcttcattgg ataaatgcaa atctaaagct tggacattgt cagatacttg ctctggacgc
    25741 ttagctccag gtataactac agacactcgt ggattcttca tgtaccaagc taaaacaagt
    25801 tgcgcaggag tggcttggta tcttttagca atggggcgaa gtacatcgac agcggttaaa
    25861 atttctctga aacgttctcc ttggaaatct ggatttcttt ttcgaggatc tccttcacca
    25921 aattgttttt cctcaccaag ttcatatttt cctgtcagca aaccggaagc aagaggaaaa
    25981 tagggaacaa aactgatttt gtttttttca agataaggga ataattcttt ttcagcctgc
    26041 cgatggatca aactgtattt atcttctact acatcgacat atccatctgc attcgcttct
    26101 ttcaattgtt ctaacgtgaa gtttgacaca ccgactgctc ggatttttcc tgcttctttt
    26161 aattcgtgca atgtagcaac tgattcattt tttggtgtgc tttcatccgg aaagtggata
    26221 taaaaaatgt cgatgtaatc agtttgcaga cgttttagtg catcttccac cgattgtttg
    26281 agaaactcag gagaattatc aaatgttcgc cctttatttg gtacatgagc agcttttgta
    26341 gcgatgacga cacgtgagcg atcatattct ttttccttca gtacctcgcc aatcagttct
    26401 tcggaacggc cgtttccgta agcataagcg gtatcgatca aattgatccc gctattcaaa
    26461 gctgtacgaa ctacttgttt tcctgtctca tcttccaaac cgtcaaaaag attatgtcct
    26521 ccaacggcat ttgctcctaa tccaagctgt tctgcgtaga cttgtgaatg tccgattctt
    26581 atttctgcca tagctgattc ctcctttttg tctagtctaa aacaaaatac caaaaagctg
    26641 aactaaaacg tttttgtgag gggaaatagt cagatatctc ttgactttga gactggtttt
    26701 tagtaggtta taagtaatat cgaatgtcaa tgaaagagcg agtaagtttt tgaaacttta
    26761 caagagagga tcatcgaggc tgaaaatgat ctaaagtttc attagcctaa agacactctg
    26821 gagatggacg agtgaaaagc cgtcccgcgt acgagcgtta ttcgtaagag gaaagagaaa
    26881 tctttcaaaa ttaggtggta ccgtgtatga atttcattca cccttgttca tgatgaacaa
    26941 gggtgatttt tttatttaca tagctaagaa agggagagaa aactatgagt ttccaaagac
    27001 caaaaggaac aaatgatatt ttgccaggcg aatcagaaaa atggcagttt gtcgaagaaa
    27061 cagcacgttt attattcaga gattatcagt ataatgaaat gcgtacaccg atttttgaac
    27121 actatgaagt gatatcccga agtgtagggg atacaacgga tatcgtttca aaagagatgt
    27181 atgattttta tgacaaaggg gaacgccatg tgacgcttcg accagaaggt acagcaccaa
    27241 tcgttcgttc ttttgttgag cataaattgt tcggcccaga gtatgcgaaa ccgttcaaaa
    27301 ctttttatat gggaccaatg tttcgctacg aacgccctca aaaaggacgg ttacgtcaat
    27361 tccatcaaat cggagtagaa gcatttggat cagaaaaccc tgcaactgat gtggagagca
    27421 tggtgatggc acttgatttc ttcaaacagt tggggatcac gcaaatccgg ctagtcatca
    27481 attcattagg tgataaagaa acgcgtcaag cctatcgtca agcattgatc gactatcttt
    27541 taccttttga atcagagctg agtgaagact ctaaacgccg ccttcatgaa aatccattac
    27601 gcgtattgga cagtaaagac aaacgagatc aaaaatttgt ggcagaagct ccatctatct
    27661 tagattatct aagcgagcct gctcaaaaac attttgatac cgttgtttcc atgctggatg
    27721 cattggctgt tccttatgaa atcgacagta atatggttcg cggactagat tattacacac
    27781 atacgatttt tgaaatcatg agtgacgcac caaaaatggg ggcacaagca acgatttgcg
    27841 caggcggacg ttatgacaat ctagtggaag aactaggtgg tccttcaaca cctggcttcg
    27901 gatttgcaat gggtatcgag cgattactct tgactatgga ggcagaggaa gtagttatcc
    27961 ctgcatttaa tgagttggat gcttacgtgg tcgcactcgg agacgaaaca aatatcgaag
    28021 cattgaaagt ggtccaagcg attcgaaact ttggtttttc agctgaccgc gacttcatga
    28081 accgtaaagc aaaagcgcaa tttaagacag cagataaagc gaatgccaaa ttagtattgg
    28141 tgatcggtga agatgagctt gctagtcaaa cgattaatgt caaagcaatg gcaagccgaa
    28201 aagaaaaaag ttttccatta gaagagatat atgaacattt tgatgaagtc tatgatgaaa
    28261 tgactttatt cgagtaggag gaaaaaaata aatggcacaa agaacgatct attgcggatt
    28321 agtttccgaa caacaattag aacaaacagt tacattaaaa ggatgggtac aaaaaagacg
    28381 agaccttggg ggagtaatct ttatcgacct acgtgatcgt gaaggaatcg ttcaaatcgt
    28441 ctttaatcct gaaatcaaca aagaagcatg ggaaatcgcg gataaatgtc gcagtgaata
    28501 cgtgatcgaa gtgactgggg aagttagaag acgcggagaa aatgcagtaa atcctaaaat
    28561 ggccactggt gaattcgaag tgattgcaag tgaaatcgtg attttgaatc aagcaaaaac
    28621 cccgccattt caaattgaag atgatcaagc aatcagtgac gaaatacgtt tgaaatatcg
    28681 ttatttggat ctgcgtcgtc caaaaatgac taaaaacatc aaattaagaa atgcgacaac
    28741 aaaagcgatt cgtcattatt tagatgataa tgactttctc gatatcgaaa caccttacct
    28801 agggaaatct acaccagaag gcgcaagaga ctacctagtt ccttcacgtg tccatgctgg
    28861 acatttctat gcgttgccgc aatctcctca attgtttaaa caattgctga tgaatgctgg
    28921 attcgaccgt tattatcaaa tcgttcgttg tttccgcgat gaagatttac gtggagaccg
    28981 tcagcctgaa tttacgcaag tagatattga aacaacgttc ttaacagcag aagaaatcca
    29041 aacttacaca gagggattga ttgcgaaggt aatgaaagaa gttcgcggta tcgaagtcac
    29101 attgccattt cctcgtatga cctatgatga agcaatggct cgctacggca gcgacaaacc
    29161 ggatacacgt tttgatatgg aattgatcga tctaagcgat acagtcaaag aagtagaatt
    29221 caaagtcttc caaatggctc ttgaaaacgg cggtgtcgtg aaagcattga atgctaaagg
    29281 cgctgcagat cgctactcac gaaaagacat ggatcagtta ggtcaatatg taggacaatt
    29341 cggagctaag gggcttgcat ggctgaaagt agaagaagac ggattgaaag gtccaattgc
    29401 taaattcatg ggagaagcaa cagaagcaat cattaaagca acagatgcaa aaccaggtga
    29461 cttgctgatg tttggcgcag ataaaagtga aatcgtcgct gctgcattag gtgctatccg
    29521 gacacgttta ggaaaagaat taggactaat tgatgaatcg aaattcaatt tcttatgggt
    29581 aactgattgg ccacaatttg aattttcaga agaagaaggt cgatatgtct ctgctcacca
    29641 tccattcacg atgccaaaag aagaagatat cccgttattg gctgaagacc ctgcaaaagt
    29701 ctatgcagaa gcttatgata tcgtcttgaa cggttatgaa ttaggcggag gttctatccg
    29761 tattcatact cgtgagcttc aagaaaaagt actagaaact ctaggcttta caaaagaaca
    29821 aatggaagaa caatttggtt tcttgttaac agctttagat tacggattcc ctccacatgg
    29881 cggtattgca ttaggattgg accgttttgc aatgttatta gccggagaag ataatattcg
    29941 tgaagtcatt gcctttccta aaaacggaaa agcagcagat gtgatgagtg atgcaccaag
    30001 tatggtttcg cctttacaat tgttcgaatt gaatattgat gtaacgaata tagaagaata
    30061 gccgataaaa aggtaaaaag gatttgtcga atacgcttct tttgacaacc ttgatttaat
    30121 tgaaaaacac tttataatga agccataaca tataaagcgg ggcttttcca tgaaaaaaac
    30181 attcgaaaat tgggcttatc atgattacac gaccaagctt tcggtatcta tcgtttatgc
    30241 gttgatcgca tcagtcgcat tgaatttctt ttatcagcca ggacatattt attcaagtgg
    30301 tattactggt ctagctcaga ttttgacgac tttgtcaaaa tcactgatag gattcgaagt
    30361 accggtatca acaactttgt atttactgaa tatacctttg tttgtattgg cgtggttaaa
    30421 aatcgggaag aagtttacaa ttttcacttt tatcacagtc actttgacct ccctttgtat
    30481 ccatatttta ccggaaacag tcctatctac cgatccaatc atctgtgcgt tgtttggggg
    30541 ggctgtgatg ggactaggta ttgggctagc gttgagaaac ggcctatcat cgggcggtct
    30601 agatatcgtg agtatcacga ttcgtaaaaa aacaggaaag tcgattggtt ctatttcgat
    30661 ctacttcaat gcattgatcg tttttgttgc cggaattcta tttggctggc aatatatgtt
    30721 ttacagtacg ttgtctattt tcgtcagtgg aaaagtaact gatgtcgttt atactaaaca
    30781 aaagaagatg caggtaatga tcgtaacgaa acatccagaa aaagtagtaa atgaaatcca
    30841 aaactgtatg cgtagaggga tcacgatcat caatgaagca gaaggtgctt ataagcatga
    30901 taagcagaca gtattgatga cgatcgttac acgatacgaa ctgcctctac ttagagaagc
    30961 aatgaaagct tctgatccac aagcttttgt aagtattgca gaaaacgtcc agatacttgg
    31021 tcgtttctat gaggatggca tttagatcga gtttgacaaa aattcatgaa atactgctgt
    31081 agacagacgt tcgtttgtct gcagtttttt tctaccatag tagaatcagc aaagaaaaac
    31141 tgctttttta tttgaaattt tcttaaagtt gaaaaaaagc atgtttattt ttttaaattt
    31201 atgggatgat agaaaaaaat agtagagaaa ggacacgatt ctacttgcta ttttcactta
    31261 atacccgtat attacgaatg aaaaaagttt atgaaaagtt ggaatattga atggaaaaac
    31321 aaaaaaggtt aaaaaacagc cgctacatca ctgggtttga tgggctccgt tcattagctg
    31381 tggtgggtgt tatcttctat caccttctgc caactagtat gaaaggcggc tatttgggcg
    31441 ttccgatttt tttcgtcgta tctggttatt tgatcacgga tctgctgcgg caggaatggg
    31501 aacaaaatgg aaaaatcaat atttggcaat tttatgtcag aagaatgaag agactctatc
    31561 cggcacttgt ttttatgctt gtgacagctt cggcttatat cactttgttt cagcgagggc
    31621 tgttaaacaa tcttcgagga gttgtggtca gcagcttact gtatgtcaac aactggtggc
    31681 aaatcaataa cggtttatct tatttcgatc gatttgctaa tgagtcaccg tttacgcata
    31741 tctggtcttt agcagtagaa ggtcaaaact atttggtttg gccgatcttg tttgtattgt
    31801 tgatggtatt tgttcgtaaa agaaaatgga tcttttatac tgtgttaggt acatccttcc
    31861 tctccgccat cttgatgatg atcttgtata caccaggagg cgatccaacc cgtgtttatt
    31921 acggtaccga cactcgtctg ttttctatat ggttgggaag tgcgcttgcg tttgtttggc
    31981 caagtacgcg tttaaagaaa aacatcccga aacaagcgaa aaatattctg aatatcgctg
    32041 gaggaatctc gcttttggca ctgattcttt gcttcttctt tttagatgat cattacaatt
    32101 tcatctatta cggaggaatg ctcctagtga gtctgttatg cgtagtgcta gttgcagtga
    32161 cagcacaccc cggagcaagt ttgaatcgat ggctgacaaa tccagtattt acttggattg
    32221 gcaaacgaag ctatggcatt tatctgtatc agtttccagt tatgattttt tatgaagcaa
    32281 aggtgactga tattgccgat catgttttgt tgcatacttt agtggaaatc acattgattc
    32341 taatcatcag tgaactttct tatcgatatg tggagcgtcc gctagcacgt tttgattatg
    32401 gtcgtacatt ccaagtgatg aaaggatggt tcagcaaacc gatcatcagt aagagcaaac
    32461 catgggttgt tccaggcaca ttacttgtag caattgcact cgttggtttc gttacggcac
    32521 cgaaaaacgc tgtaacagcc gatcaaaaac aactgcaggc aaaaatcgct gaaagtaaaa
    32581 aattagcaga agaaaccaga aacaattcag aaaacaccac tagttcggtt gaccaagcga
    32641 tccttgataa gtacgagttg tccaaggcac aagggaataa ggcacaaaaa ttagaactaa
    32701 cggcatttgg tgattcagtt atgttggatg ctgcaacgga tctacaagaa ctttatccaa
    32761 aagtagtggt agatggagat gtcggacgac aattatatgc cagtgtaccg tacattgaaa
    32821 aattaaagga acaagatttg ttgaaagaca ctgttctgat tggactagga acgaatggct
    32881 cattctcgga agctcagttt gatagtgtga tggatgcaat cggtgatcga aaagtatact
    32941 gggtcaatgt ccgagtaccg acgaagcgct ggcaaaatga cgtgaatgcg atgcttgaac
    33001 agatgacgaa aaaatacgac aatatgacgc tgattgattg gtacagctac agtaatgacc
    33061 atagtgactg gttttatgat gatcaagtcc atccaaatcc tgaagggatg acacattaca
    33121 ttcacttagt cagcgaagcc ttgctaggag gaagttcaag tacgaacagc agtgagacaa
    33181 acgtgacaga atgatagcta caaaaaactt caggcaaaaa catttgtttt tgcctgaagt
    33241 ttttttatat ttaccgtgtt tttaacattc atatagattc aggaagcaaa acggttgagt
    33301 gtgtctgtaa ataacgttct ttttgttttc gggtgagttt tttgaatgca gcttctgctt
    33361 ttgttgcttc gcttcttgaa gaaaatactt ctgcatggat catcttaaca ggtcttcttt
    33421 tttgaagacg agtatattta gcaccagtac cattgttatg ttcctctaat cgtctagaaa
    33481 gatcggttgt atatccacca taaaatgtct gatctttaca gaataatacg taaaaataat
    33541 gttcatttgc catagagcat ctcacgaatc tctgcagtgt attctccatc catattgtaa
    33601 gtgatcaatg gtggagcgac tttgaatccg tctaatttgc cttctttgat tccttcaact
    33661 aacaaaatat ttgcctcttt tccaggtttt gggtaaacaa attggatcct tttaggtgca
    33721 attcgatagt cttccatgac ctgaatgatt tctaagaacc gatcaggacg atggaccata
    33781 gtaaagcgtc catttgtttt tagtagctta cttgaaatat cgatcacttc ttctaacgtc
    33841 gtatgaatct catgacgggc gattgccagg tgtttattag ggttcgtttt atttgttggc
    33901 agccctttga aataaggtgg attacagacg actaagtcac aagaattatg tcgaacgaca
    33961 gataagctgt ctttcagatc gatggtgtgg acagtcattt gttcatttaa atggttaagc
    34021 gcgatactac gtcttgccat gtctgctaga cgaggttgaa gttcgatagc gtcgatagcc
    34081 gcctgggttc gtttacttaa aaaaagtcca actgcgccat ttcctgcaca aaggtcaaca
    34141 atcttccctt ttttaggtac acgggaaaag tgagcaagca gcacagcatc taaagaaaaa
    34201 gaaaacactt tggaactttg gataatcttg atatcttctg cgtataattg gtcgattcgt
    34261 tcgtcttcgt gaagcatgga ttccctccaa aatgaaaaaa atagtcgtct gtattatact
    34321 atttttatta aaaagaacca aggtggcttg tcaaacctct attttttatg catactatga
    34381 ccataagaag ggaaaggaag agactatgtt ttatcgattt atgcgcgggg tagttcgagt
    34441 actattagcc ttagttaatg gaaatgcccg ctatgagaat aaagaagttt taccgcaaga
    34501 tgagaattac atactagttg ctccccatcg tacttggtgg gacccattat atctggcggt
    34561 agctgcagca cccaaggaat tcagctttat ggcaaaagaa gaactgttca aaaatccagt
    34621 tctgcgattt atattaactc gatccaatgc ttttccagtt aaaagagaaa atccaggacc
    34681 aagcgtcatt aaaacacctg taaagatttt gaaatcgaca aatttgagtt tgatcatgtt
    34741 tcccagtggg acaagacatg ctacagaact taaaggcgga atggcattga tttccagaat
    34801 gtctaaagcg aaaatcgtgc cagctgtata tcaaggtccg ttgacacttg gggatctgtt
    34861 caagcgtcag agagtgacag tgcgtttcgg cgaaccaatc gacttatcag atatcaagaa
    34921 aacggataaa gaaggtatgg cggagatcga acgacgaaca caagcagctt ttgacaaatt
    34981 ggacaaagaa gtgaaccctg atttcaaata tgaagtcaaa aaaaaataaa atgataagtt
    35041 tatgcgtggg cggtcttttg aatctactgt cctgttcagt tcagactttt tcaatgaaaa
    35101 cgagggagag acgtcaagtc tgccctcttt ttgcgttcca atatgtaaaa tagaagacag
    35161 gtgtcatgta atggatcaaa gtgttataat aattagaagg acattttttg aggtgattga
    35221 atgcgttttt tacatacagc cgattggcat atcggaaaaa aactacaagg ttatgacttg
    35281 attgaagacc agcgtcatgt actatcttct atactagata tcgcgaaaga agaaaaagta
    35341 gaggcaatcg tgattgccgg agacttatat gataggagtg tcccagctgt tgatgccgta
    35401 gaattataca atgaattgat ggtcgattgg aatttaaagg aaaagatccc gatttttgct
    35461 atatcaggaa atcacgatag cagtacgaga ctttcagcgg gtacgccatg gttcagttat
    35521 tcgaactatt ttttgcgaac acagctagca gaagcatttg aaccagtcga gtttaaaaac
    35581 gtacaatttt tctttcttcc ttactttgaa ccaattgcag cgagattata ttttgaagat
    35641 gacaatatcc gaacgatcaa gcaagcggta gccaaggtga tagataaaat gcaggagcta
    35701 tttgattcag aaaaaaaaca agtattgatt agccattttt ttgtaatggg aagtgtaaaa
    35761 tctgattcgg aaacgaaaat cgaagtaggc gggttggatt cgatccctgg tgaactactt
    35821 acaccgtttg attatgtagc actaggacat ttgcataatc aagctgcttt gagacaggat
    35881 aatgctcgct atagcggttc cccattgaaa ttctctttat ctgagatcaa ccaaaaaaaa
    35941 ggcgtatgga tcgttgacct gacaacaggc ctggagttga attttaggga gatcgaaccc
    36001 ctgcatgata ttgaagaaat aacagcgagt ttcaaagaat tgctggatcc tgccttttat
    36061 cagtccgtca atcgggagaa ttacatctat atctatctca ctgatagaac gatcatcccg
    36121 aatatgatga accagcttcg gcaaatatat cctcgtattt tggggattga acgggcaaac
    36181 ggtcgagcag aacaaacaaa gcgcaaacga aaagtactag aaatgaaaga tcctcaaaaa
    36241 ttagcagaag cattttttag tgaaatgacg gaagagcagc taactgcaaa ccaagaaaaa
    36301 tggctcggag aaacgttgca agaaatcatg gaaatggaat gatcaagaat gaaaccgaaa
    36361 aaattacaac ttaaaaattt tggtcctttt atcaatgaga ccgtagattt ttcacgtttg
    36421 acagaagcac cgcttttttt gatcagcggg aagacaggag ctggcaaaac gacgattttt
    36481 gatgggatca ccttcgcact ttttggtgaa acttccggaa gacttcgctc tggaaaagag
    36541 atgcgttctt tgtttgctac accagacgaa gaaacaagcg tgactttttc ttttgaacat
    36601 cagcagatga gctatgaaat cgaacgaaaa ccagaacaag ttttagcaaa acgaaaagga
    36661 aacggaacaa aaaaacaggc agccaaagta acgttgacca tttttgatgg gaaaggaaaa
    36721 gaaataaaac aaatcacaaa acgtacagaa gcagatcagt tgatcagaga actgatgaat
    36781 ctcgatgcga aacagttttc tcaaatcgtt ttactacccc aaggagaatt ccgtaatttt
    36841 ttggtttcat caagcagtga taaagagact gttttacgtc atttattcgg tactcaattt
    36901 ttccaacagt tcaatgagcg attaaaagaa aaagcaaaac aacagcaaca aaaattggat
    36961 catttagagc aagagctgca tcttctgcaa aaaaggtttg ttttagtcaa agaagaggaa
    37021 ccagcagcag ggtttgaagc cgttttagcc caatggacaa accatcaggc aatactcgaa
    37081 aaacaaatca cggaagaaaa agaagctcta acggaacttc aggaaaaaca aaagcaacta
    37141 gaaaattcct actatcgttt tgaaagcttg aaaaaaagtt atgaagaaaa aacgatatta
    37201 ataaacaagc aaaaggaatt agctgctcaa aaagacgaaa tagaggaaaa gaaacgatgg
    37261 attgggtatt atgaatttac gcaacgatta gtcgaaccaa tccagcgcct acaagaaata
    37321 aaagttgaaa aaatagaact gtcacaaaaa agtagtgagc aaacaaagaa actagcgatg
    37381 actacgcaga attatgaaga gtggcaaaaa aatgaagcaa accgcgtacg ccgacaaaca
    37441 aaaatcaaag aagctgctca aaaactgcaa aacaaacaga tgatggtgcc aattgtcgaa
    37501 tccttacgta gtaaaaaaca agacgcagaa caattaacaa tttcattgga taaagaagaa
    37561 ttgaatgccg agaagctgta tcaaaatctc gctatctatc aggaaagaac tcgaattctt
    37621 caagaaaatt tactggatca ggaaaaactg caagaagatc gtcttgcttt tggacagtta
    37681 aaatatctag atgaaaagca gcaagaaata gaaaaacaaa aagcaaaaga cactgggtcg
    37741 ctcgcacaga taagagaaga actcaaaaaa gcaactgaag aaataacaaa aaatagtcag
    37801 ctattgaaag aacagacaga acaagcagaa cagctgaaaa gccaatgggc aaagcagcaa
    37861 attgctcgtc ttcagctttt tttgcgtcca ggggagccat gtcttgtttg tggatcaacg
    37921 gaacacccca tacaacaatt aaaacacgaa cagccaaata aggaagatat cttaaaagta
    37981 gaacaacaac tggcagaagc tgagtctgct gtagaaagaa ctcagcaaat cattgccaaa
    38041 agtgaagcac atgaaaataa tcttcaacaa caattatcag atttagaaca aaaaatcatg
    38101 gcaacgaata tacaattggc tgaacaagaa gaacacttgc aacaggcttt gaaaaaggca
    38161 tttcctgaga taacagaaca aaaaacaaaa acaactcttg tagtaatcga acaatcttta
    38221 gatagacgaa aaagcaagat ggccaatgaa agaatagagc tggaagaaat aagacaacaa
    38281 gcagaaaagc tcgaacaaca attagctgtc tacaaacagc agcagacaga aaaaaataat
    38341 cagctccagc aactgcaagg agaaatcaaa agtttagaac aacaattgca agatacggat
    38401 agtaactgtt tagaagagga gatacgacga cttgaaaaac agttagaaga agactcgaat
    38461 caactagctg ttgagcagaa agcaggagtg caattgttgc aagatcttca tacgcttcgg
    38521 acacagcttg atcttcaaca tgaacaaata gaaaaaatag atgaaaaaat agaaaaggaa
    38581 caagaaagaa tagctaaaaa gttagaaagc cagtcgtatt tttcaacaat tgaagaaatc
    38641 tgtgcatttg cacaacagca agcacaatat aaagaaaacc aacgtacagt tacttcttat
    38701 catgaagagc aattagtgac agctgaccgg ctgcgtcaat tagaaaatat ccagctaact
    38761 gagacattcc cagatacaga agagatcagc caacagttag aagaaataag acgtatgatc
    38821 gaaataaagc aagaaaagat atacggttta caagaacaaa aaattagtaa tcaaaaaatc
    38881 tacgatgaat gtctttcgat ttatcacaca agccagacga aattagatga gcttacgcaa
    38941 ttacagcagc tttcgcaaac attcaacgga gaaaatccta agaagactag tttggaacgg
    39001 tatgtgcttc aggtttatct tcaagaagtg ttacaagtag caaatgatca tctccaacgt
    39061 ttaacgaaaa atcgttatca atttgagcta gcggatacca ttggcagtta tcgagggaaa
    39121 accggattag aaatcaatat ctatgatgat caggcaggta tgtctcgaag tgcgcatacc
    39181 ctgtctggcg gtgagagttt tattgcagca ctttctttag cattagctct tgccgatgtg
    39241 atccagacac aagctggtgg tgtagcaatc gatgcattgt ttattgatga aggatttggg
    39301 tcattagatg aagaagcttt ggaaatggca atggaagctt tagaaacgat cgaaagtgaa
    39361 ggaagaatga tcggcatcat tagtcatgta agagaattga aagaacgagt attacaacaa
    39421 ataagaattg atacagaagg tagcggacaa agcaaaatac gctatcaact tggataaaaa
    39481 gggagattgg taactatgaa atggagtatt ccagaaaaag tcattgcacg tggacgtcaa
    39541 tacttgcaag aaggccgagt gttatcggtt gtccctgacc aagaagaaaa cgtgtggcat
    39601 gcagaagtct tgggcagtga attgtatatg gttgatttgg atggtacagc aaaagagata
    39661 gattactgcc aatgtcccta ttgggaggaa cataaatatt gtaagcatac agttgctgtg
    39721 gaactttatt tacggtcgaa aaatctatct cgtatcatga aaaaagacca gccaatgcta
    39781 gaaccggtaa aagcaacaag tgagggagaa atcattgcaa aaggtttttc tcgtctaaaa
    39841 acacaaaaca atcctcaagc ggtgcaacct ttgattattg aataccaagt agaaacgatt
    39901 gagactaatc agtatcaccc agagctttct attttggcac tttacttgcg tatcggttca
    39961 ttggcatcac cgaaaaaaac atatatcgta aaaaacatct atgatttttt acaagcttac
    40021 acagaaaaag aaacttacac tgttaataaa cagtaccagt ttcgtctaga taaacgggcg
    40081 tttcaaaaag aagatcaaaa aatactcacg catcttgcag gaagtgctca gacgcatcaa
    40141 ttattaggta cgaacggctt acaagtcaaa ggaaaactag ataaaagata tctgcttctc
    40201 ccagtagaac aagcccgctt cctgttagag cagatgaatc agactacacg tttgcatctg
    40261 cagataaatg aacaaaacga tcaaggaata caattttctg atcaaaacaa acctttatca
    40321 ttcaaagtgg aaaaatttgg agaagattat ttgctgcaag cattgaatga ttttgatttt
    40381 ttctttatgc attatcagtg gggagttatc caagggacat tttattctct gacgaaatat
    40441 caacaaacgg tttatcaaac ttggaaacaa ttgattcgcc gattggagaa accagaagta
    40501 gtgttcaaga aaaaagaatt accgtctttg tttaaagaga ttatccctct gctgtcagag
    40561 atcggcgacg tagctattgc ccctgcagta gaagaggaag tagcagatat ccccgtcgaa
    40621 tttattttct ttttccgaaa agcaaaagga aaaatccaag ctcgagtcga tttcgtctat
    40681 ggtgaagtca tctattcgac tgacccgaag catgaggtct cgacagaaca ggcttttcag
    40741 gtgttacgtg acacagtaga agagaaacga gtacttgatc tattcagaat gtatcgctac
    40801 cagaaaaatg aaactggtta tgaacgtcca ttgccaagcg gggaagaact atatgccttt
    40861 ttccgcacag aactatctgt cttccgtcag tttggtgaag tcagacttgg aagaaaattg
    40921 cgagagttgt atttagatgc gcataaacat caaccgaaaa tcgaagtaac cgagaccggt
    40981 tcatggctcg atattcgatt cgatgtgaca gggatagagg agcaagaaat cgatcatgtc
    41041 ctgcaaagct tgctgagaaa tgatgctttt tatacattag aaaatggtca ggttctttct
    41101 tttgattcag aagaatttca acaaacaagc caagtgttgc aacaattacg ggaatcgata
    41161 cgtacagaag aaggaacgat ccatgtacct aaaaatcaag gattgatcat tcaaaatcaa
    41221 ttggaaaaaa gcaatgctac ttttagtgaa tcgttccaaa cgatggtaca agatctcatt
    41281 catccagaaa gataccaggc acagcttccc aaaggattga atgcaacgat gagagattat
    41341 caaaagcaag gttttcgatg gttgaagatg ttaggtcatt atcagtttgg cggtatttta
    41401 gccgatgaaa tgggacttgg gaaaacacta cagacaattg cctttttact ttcggaaaaa
    41461 gaagaacgga aaagcttctc tgctttgatc gtagcgcctg cgagcttaat ttacaactgg
    41521 caggcagagg tgagaaaatt cgccccttct ttaagtatcc aagtaatcaa tgggaataaa
    41581 aaagaacgtg aagaattact ggctaaagac acagatatcc gagtgacttc ttatgccagc
    41641 ttaagacagg atctagctaa ttatcagtcg caaaaaattg attatttgat tcttgatgag
    41701 gcacaaatgg tcaaaaatag cagtacgaaa actgcacaag cattgagaga attagctgta
    41761 cctcagcggt ttgcactgag tgggacacca atcgagaata atttagaaga attgtggtca
    41821 ttgtttgcaa cgattatgcc aggatttttc ccgactaaaa caaaattcag ggaacttgcc
    41881 gcagaagaaa ttgcgcaaat gattcgaccg tttattttac ggcgagataa acaaactgtg
    41941 ttgaaagact tgccggaaaa aacagagatg aatctatatt ctgcacttac agaagaacaa
    42001 aaaactgttt atttagctta cttgcgtcaa atgcgtgaag aaatcacatc aatggattct
    42061 gaagcattca agaaaaaccg gatcgggata cttgctggtc tgacaagact gcgacaaatt
    42121 tgctgcgatc ctcgtctgtt tattgaagat taccaaggcg gttccggcaa gctcgaacaa
    42181 gtaaaagatc tactggtagc tgccaaagag aacaaacgaa gagttttgct gttttctcaa
    42241 tttacaggga tgctgactat cttgcaagaa gagttagccg agctagggat ctcgacattt
    42301 tatttaagag gcagtacgaa accacaagat cggttaagta tggtagacgc atttaatgct
    42361 ggcgaaaaag atgtttttct gatttcctta aaggctgggg gaactggatt gaatctcacg
    42421 ggagcagata cggtcatctt gtatgacctt tggtggaatc ctgccattga agaacaagct
    42481 gctggacgtg cacatcggat cggacaaaaa aatgtggtag aagtgtggcg tatgatctct
    42541 gaaggaacga ttgaggaacg catggattca ctccagcaag aaaaacgcga actgttccaa
    42601 aaagtgatcc aaggtaatga agaacagttg acgaaactga ctgaagaaga catccgcatg
    42661 attttaagtg taggagaaat cgaggagtaa gagactcgaa aaagcgttca gtaatgggta
    42721 agaaagcctt tctaaagaag cgattgttta tttgatttaa aaaatagcaa ggaggctggg
    42781 attccatttt atcccagctt ccttgctatc atttttttat ttctattttt aaaggttata
    42841 caaagattgt cttcattgtc tctaacagct gatcaaaaga agaaatctcg atatcgcaaa
    42901 ttggagtctg ttcaattttg cgttctcgat ggttgaacca aagcgcttgc cagttagcac
    42961 tttttgcacc tagaacatca ttgtcgtaat tatcgccaac gtataaggtc tcttctggaa
    43021 gcatatgaaa ttctttctca gccagctgaa aaatctcttt ttctggtttt tggaaacctg
    43081 ttgcttgaga aatgagcata ttgcgggaag gaatccaatt gttcaattga agttgcttca
    43141 gtttttttgt ctgatggtca gtcggcccat ttgtgataat tcctaatggg acattctttt
    43201 ccttcaagaa atcgagggtt tttttgacct cctcatgcaa gcagatatta tcaagttctt
    43261 cctcgtaaat tttttgaaat aagaggccat cttcttccgt aatatgaggg tagtctaaat
    43321 cttttagtga ttggctgatc cgatgtgctc gcatatattc tagtgtccaa tctcctgcca
    43381 ttacttttgg gaagttttca tcactatgat ggcggaaccg gatatatagt ggatgcatat
    43441 ctgcatctga aacaagcgga acgactcttt tgaccgcgtt tcgaaatggc tgctgctgat
    43501 catacatcgt atcatcaaca tcaaacacaa ctgatttaat caaaatttta cacgcctttc
    43561 ggttttcatt gattttttca caaatccttg actattgtaa cagaaaaaat tgtctaaaaa
    43621 agctgataaa taaaggtttt tcttactata tttctatttc cttgacagaa taaggttttt
    43681 atatttcagt ttttttcact aaaaatcgtg aaacacgtca ataagtttga tgaacaaatt
    43741 aatcattatg gtagaagtcg attgatttat tttaatgcaa tctctgtgga attttcgaaa
    43801 gatttatttt tttaatacag tctca
//
LOCUS       DACDRK010000014        43767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDRK010000014 DACDRK010000000
VERSION     DACDRK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.014"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(407..2788)
                     /locus_tag="IWJ77_000875"
     CDS             complement(407..2788)
                     /locus_tag="IWJ77_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9452514.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2778..3425)
                     /gene="recU"
                     /locus_tag="IWJ77_000876"
     CDS             complement(2778..3425)
                     /gene="recU"
                     /locus_tag="IWJ77_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9452515.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3489..4028
                     /locus_tag="IWJ77_000877"
     CDS             3489..4028
                     /locus_tag="IWJ77_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9452516.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4118..4537
                     /gene="gpsB"
                     /locus_tag="IWJ77_000878"
     CDS             4118..4537
                     /gene="gpsB"
                     /locus_tag="IWJ77_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9452517.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4559..4926
                     /gene="rnpB"
                     /locus_tag="IWJ77_000879"
     ncRNA           4559..4926
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWJ77_000879"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5135..6301
                     /locus_tag="IWJ77_000880"
     CDS             5135..6301
                     /locus_tag="IWJ77_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9452518.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6347..7843
                     /locus_tag="IWJ77_000881"
     CDS             6347..7843
                     /locus_tag="IWJ77_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9452519.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7924..8607
                     /locus_tag="IWJ77_000882"
     CDS             7924..8607
                     /locus_tag="IWJ77_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9452520.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9252..10442
                     /locus_tag="IWJ77_000883"
     CDS             9252..10442
                     /locus_tag="IWJ77_000883"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9452521.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10544..11497
                     /locus_tag="IWJ77_000884"
     CDS             10544..11497
                     /locus_tag="IWJ77_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9452522.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11566..12915
                     /locus_tag="IWJ77_000885"
     CDS             11566..12915
                     /locus_tag="IWJ77_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9452523.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13098..13421)
                     /locus_tag="IWJ77_000886"
     CDS             complement(13098..13421)
                     /locus_tag="IWJ77_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452524.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13550..14713
                     /locus_tag="IWJ77_000887"
     CDS             13550..14713
                     /locus_tag="IWJ77_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452525.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14783..16132
                     /locus_tag="IWJ77_000888"
     CDS             14783..16132
                     /locus_tag="IWJ77_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9452526.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16177..16608)
                     /locus_tag="IWJ77_000889"
     CDS             complement(16177..16608)
                     /locus_tag="IWJ77_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9452527.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16996..19638
                     /gene="alaS"
                     /locus_tag="IWJ77_000890"
     CDS             16996..19638
                     /gene="alaS"
                     /locus_tag="IWJ77_000890"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9452528.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19838..20434
                     /locus_tag="IWJ77_000891"
     CDS             19838..20434
                     /locus_tag="IWJ77_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9452529.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20556..21704
                     /locus_tag="IWJ77_000892"
     CDS             20556..21704
                     /locus_tag="IWJ77_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9452530.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21742..22155)
                     /locus_tag="IWJ77_000893"
     CDS             complement(21742..22155)
                     /locus_tag="IWJ77_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9452531.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22234..22665
                     /locus_tag="IWJ77_000894"
     CDS             22234..22665
                     /locus_tag="IWJ77_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9452532.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22764..22997)
                     /locus_tag="IWJ77_000895"
     CDS             complement(22764..22997)
                     /locus_tag="IWJ77_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9452533.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23180..24850
                     /locus_tag="IWJ77_000896"
     CDS             23180..24850
                     /locus_tag="IWJ77_000896"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9452534.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24997..25383
                     /locus_tag="IWJ77_000897"
     CDS             24997..25383
                     /locus_tag="IWJ77_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452535.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25370..26068
                     /locus_tag="IWJ77_000898"
     CDS             25370..26068
                     /locus_tag="IWJ77_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9452536.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26073..26537
                     /locus_tag="IWJ77_000899"
     CDS             26073..26537
                     /locus_tag="IWJ77_000899"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9452537.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26534..27445
                     /locus_tag="IWJ77_000900"
     CDS             26534..27445
                     /locus_tag="IWJ77_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9452538.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27680..28219
                     /gene="pyrR"
                     /locus_tag="IWJ77_000901"
     CDS             27680..28219
                     /gene="pyrR"
                     /locus_tag="IWJ77_000901"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9452539.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28233..29516
                     /locus_tag="IWJ77_000902"
     CDS             28233..29516
                     /locus_tag="IWJ77_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9452540.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29529..30455
                     /locus_tag="IWJ77_000903"
     CDS             29529..30455
                     /locus_tag="IWJ77_000903"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9452541.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30474..31754
                     /locus_tag="IWJ77_000904"
     CDS             30474..31754
                     /locus_tag="IWJ77_000904"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9452542.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31759..32835
                     /gene="carA"
                     /locus_tag="IWJ77_000905"
     CDS             31759..32835
                     /gene="carA"
                     /locus_tag="IWJ77_000905"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9452543.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32840..36025
                     /gene="carB"
                     /locus_tag="IWJ77_000906"
     CDS             32840..36025
                     /gene="carB"
                     /locus_tag="IWJ77_000906"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9452544.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36079..36855
                     /locus_tag="IWJ77_000907"
     CDS             36079..36855
                     /locus_tag="IWJ77_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9452545.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36852..37784
                     /locus_tag="IWJ77_000908"
     CDS             36852..37784
                     /locus_tag="IWJ77_000908"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9452546.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37781..38497
                     /gene="pyrF"
                     /locus_tag="IWJ77_000909"
     CDS             37781..38497
                     /gene="pyrF"
                     /locus_tag="IWJ77_000909"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9452547.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38504..39193
                     /locus_tag="IWJ77_000910"
     CDS             38504..39193
                     /locus_tag="IWJ77_000910"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9452548.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            39166..39435
                     /locus_tag="IWJ77_000911"
     CDS             39166..39435
                     /locus_tag="IWJ77_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452549.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39473..40114
                     /locus_tag="IWJ77_000912"
     CDS             39473..40114
                     /locus_tag="IWJ77_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9452550.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40145..40849
                     /locus_tag="IWJ77_000913"
     CDS             40145..40849
                     /locus_tag="IWJ77_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9452551.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40846..41748
                     /locus_tag="IWJ77_000914"
     CDS             40846..41748
                     /locus_tag="IWJ77_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9452552.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41906..43612)
                     /locus_tag="IWJ77_000915"
     CDS             complement(41906..43612)
                     /locus_tag="IWJ77_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9452553.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        14031 a         7707 c         9503 g        12526 t
ORIGIN      
        1 cgaggtattg ttttcgataa aaaaagatgg tgcagacaaa ttatgataat taagcatact
       61 atcagataac tcaaaatgaa gccttctttc aaaaaattat tcaaaaccat atattgtatt
      121 ttttatttga taataacaat gggattatta agaagtggca ctaaataaaa gactagtgtg
      181 tatttagccg atttaattat aaacaagtaa atggataaag tatatgtaat gataaaagaa
      241 aaatggattt tctaattttt aagctaaatg ttagatcaca catgtatatg ctggatagtc
      301 ttataaaaaa tcatgtaata cagcttggta catgattttt atttgtagct gttttttaca
      361 tagacacaaa aaagaaacca cgcttgtaga cgtggtttct tttttactat ctgtttccag
      421 aagctgaggt gctgctggat ggtgtatggt catttgcagc atccgctccg ctagttgctg
      481 gctcactgga agaagcagga ggagcagatg aagacggtgt cgagctttct tcggatgaag
      541 aagaaggaac cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg ctttcttttg
      601 aggactcagc agttgattcg ctttgactag tagagctaga gctttgagtc gttgtctctg
      661 ttgttgagct tccaggagtc tggacatagc tggaagatgg aatagttgtt gatggtgtaa
      721 ttgcattcga tctagctgta tattgatctt tgtagtacag ttcaccgccc acacgaatta
      781 atccgcttgg catctcccag tctgtatttg tcacgttagc tgaaacatac tgcataagct
      841 cgcgataaac atcagaggct acgtgtgacg attctgaagt gacaggtgtc atttttttat
      901 tgtaacctgt ccacacggaa atcgaataat ttggcgtata gcccgcaaat aggatatctg
      961 gatacacccc agaagaaata ccaagttttg catactcgtc atctgtgtaa ttggatgttc
     1021 cggtcttacc ggcttgatat aacccagcga tttgcgcatt cgtaccagtt ccttcagtga
     1081 tcgtatcttt taaaatgtct gtgatcatat aagctgtttc tggagacatg gctgtttttc
     1141 catctggctc ataagtttct tctgttccgt cttggaagac gattttatta acatattgag
     1201 gtttgtaata agtccctcca ttggcgaaag ccgcataggc tgctgccatt tttaaggaag
     1261 aagctccgta ttttgtccca tcttgttctt ccgtattact agagatcgca ttcgattgat
     1321 ggatcgtact gtattcgatc ccaagatttt ttaagaaaga agccacttta tctgaaccga
     1381 cttcattgaa taatttgact gctggaacat tacgggataa gtaaagtgct tggcgtaaag
     1441 taatggtgcc catatattga ttatcccagt ttcctacagg agtcgaagtc ccttcatagt
     1501 tatacggtgc gtcagtaata gtttttccag tagaatattt caaatattca aaagctggtc
     1561 cgtaatctgt gacaggtttc atcgtagaac caaaatcacg ggaagtattc actgctaagt
     1621 tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc aatctgggct tttactttcc
     1681 cggtattggt gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg taagagacat
     1741 actgatccgt gttgacgata tcatatagtt tcttttgggc gtccagatcc aaattcgtat
     1801 aaatctctaa accgtctgtg tagacattct tatctgtttt ttcttgtact tcattgatga
     1861 cttctttgac ataattatcg acgatcttag tattgtcatc ggattgagtt agttcttgca
     1921 gaccatccgt taccggtaca ttaacagctt gatcatattc tgtttgagag attttttcgt
     1981 tttgaagcat cgtatacaaa acagtatctc gtcttttctt cgcctgatct ggataaacat
     2041 aaggatcata ggcggaaggg gcttgaggca tcccagcaag caatgctgtt tgcggtaaag
     2101 aaagttctga taatttctta ccaaagtaca tctcagaagc tgtctccatc ccgtacaagc
     2161 cgtttgacat atacacttta tttacgtagt aggtcaagat ctcttgtttc gatttttttt
     2221 gttccaatct tacagccatc cacgcttctt gggccttgcg tttcagagtc tgatcctcgg
     2281 cactggtgga gaagaaggaa agtttgatca attgctgtgt caaagtactt ccgccttgca
     2341 gtccgccaga ggtaaagtta gagagggcag aaccgatgat ccgaataggg tcaaccccga
     2401 tgtgtttata aaaacggcga tcttctaccg acacgatcgc atcttctaat gttttcggta
     2461 attcattcgc agaaattttt tcacgctttt ctgcgcctaa atcttcgaat agttcgccat
     2521 cttgtgtata tagtttggaa gatacggtag cgtctaattt tttatccgtc aattcgggcg
     2581 catcttttgc gtaataccag aaaagtccta cacctgctaa aaagaggata cagaaaaatg
     2641 agagtattcc taaaaggatc ttgataaata atcctttttt atgtgttcca gaggactttc
     2701 cagaaccttt gccagaattc ttcttcacac tctttttagg agtaggctgt ttctgtcgtc
     2761 tagagcttct agtttgttca tttgccattc ttgctttctc cttcaataga taatgttgat
     2821 aaatagaagt caaccgcgtc caaataagga atccgcggag aaataccagt tttgatctct
     2881 attcctagtt cttcgatcaa agataaaggt agtgatttgc gtcctttttc cttttgggcg
     2941 tcccagtaag caatcaaatg aacactattc aaataaaaac agcgcttcaa agacgaaaac
     3001 cataacaaga caaagcagac cccattttgt tcgaggcatg ctttcatatg ttcgatttga
     3061 tgggcatgga agtttttaaa gggaaaagat gttttgttct tggtttcttt tgcttcaaag
     3121 tccaagtaaa agcctcgata gacgccatta tagtctgtag ttgaggcttg ggtaaagtag
     3181 gcttccttga tcacagcagc acttctctta ggatagtcta ctttcacgat ttggataggg
     3241 gtaggctttt tatggataac cgccaacccg cgggaaagat aatatttatt actttcattg
     3301 attgcttctt caaagcgcat cccccgattg ccgaattcag tgatctgatt tcttttttct
     3361 tgaatttttt cgacgggaga ggcattctta taataaggtt ggccatttgg atagcgcaat
     3421 accatcttta atcacactcc taggccacat tatagcaaaa aagcggatag aaagaaaagg
     3481 aatctgagat ggataagtca aaagtaatct atgtgtctgg gtatcgaagt ttcgaactcg
     3541 gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa aaatgtttta aaaaaagaac
     3601 tccatcagtt agcagaagaa gggctggaat gggtacttgt cagtgggaat ttgggcgtcg
     3661 aactatgggc tgtgcaaata gtagcggaat tgaaagaaga atatccggaa ctatctcttg
     3721 gtattttgta tcctttttct gattttggta ataactggaa tgaagggaac caagaaaaaa
     3781 aacaattagc agaacaatta gcagattatg tcgaagctgt tagccatcaa ccctaccaat
     3841 cacctagtca gctaaagaat cacactcgtt ttatacttga gcatacagtg ggctgtctgc
     3901 tgatctatga tgaagaattc cctggaaaaa cgaagtattt ttttgaagat gcacagaatt
     3961 accaagcaga tcatccctac gaaattcggc tgatcagtat ggacgatctt caaaatttta
     4021 gtgaataatc gatattgtgt ttgatttatc aacttttttc ctatacaatg aaaaaggtag
     4081 aaatgttatt acgtaaatga aaactagagg tgtaaatatg gcgaatttgg tttatagtcc
     4141 taaagacatt ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc ccgttgaagt
     4201 tgatgaattc ttagacaata tcatcaaaga ttatgaagct tacagcaaag aactccttgc
     4261 tttacaagaa gaaaacgatc gattgagtgc aaaagtagct caattatcta aaactcaagg
     4321 agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag tgaccaattt
     4381 tgatatctta aaacgcttgt caaatcttga acgtgaagta tttggtaaaa agcttgatca
     4441 acaagcaagt gcagtcaaac cagcacaacc taatccaaat aactacacaa atgcagacac
     4501 aagcttagat gataatgaaa aaacacgtca attttaattt ttgattaatg aatttgcggt
     4561 tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa gtccatgctc gcacaggctg
     4621 tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta aggtactctt tggagtaacg
     4681 gcaggaaaac aagctaaggc ttcggctatg cttaagtatc cttgaaagtg ccacagtgac
     4741 gaagcggctg gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg agcaacccaa
     4801 acaatggtag gggcgctttt tgtctggaag aagaacagga caaaaaggca gtataactgc
     4861 agatagatgg ttaccgcttt gttttttctc cctggaaaag caaagtacag aacatggctt
     4921 atagaaaacc gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt tataagaaac
     4981 aagaaaaaca tttgaaagct atttttcaat acttcagatg aatcgttctt attacaaaac
     5041 aaacttttgt atcaccggtt attcggaatt tgagtaaaag tgtggggtag gactgatgtc
     5101 ccacctcctt tttttataaa taaggagatt gtgtatggaa aaaaacagga agttcaacct
     5161 cgtcgcaact gcggcaagcg ggctagaagc actggttggt aaagaattaa gagatcttgg
     5221 aatcgaatgc caagttgaga acggacgtgc acgttttgaa ggaacaatgg aaacgatcgc
     5281 tacagctaat ttatggttac gtacagcaga tcgagtaaaa atcgtggttg gggaattcga
     5341 tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg aagattttct
     5401 tccactagat gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac tatatagcac
     5461 acctgattgc caagcgatca caaaaaaagc aatcgttgaa cgtctccgta cctattatca
     5521 ccgtccagct tctgtaccac tgacggaaac tggcgcacat tttcagttgg aagtagcttt
     5581 gctgaaagac catgtgatgg taactctgga tacgacagga ccaagtttat tcaaacgtgg
     5641 ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag cacttattat
     5701 gttgacaaat tggcgaaaag accgtccatt ttatgatcct gtatgtggtt caggtacgct
     5761 ttgtattgaa gcagccttaa taggccataa tatcgcacca ggattcaatc gtgagtttgc
     5821 ttgcgaatct tgggattggt tcagtcaaga aatcatggaa aatgttcgat cagcagccga
     5881 agaaaaagca gattatgata ttgaattaga tattactggt tcagatatca atggacggat
     5941 gattgaaatt gccaaagcca atgcagaaga aatcggatta ggctcttcga ttacctttaa
     6001 gcaacaagca gtcaaagact tcaaaacgga taaagaatac ggggtcatcg tggctaatcc
     6061 gccttacgga gaacgtctag gagaagaaga aactgttcgg aaattatata aagaaatggg
     6121 cgatgtattc cgtccattaa aaacatggag taaatatatc ttgactagcg acttagcgtt
     6181 tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg gtgcattgcg
     6241 tacggatcta ttccagtatt ggggaactcg tccgcctaga aaaaaagaga aagacgaata
     6301 atcttactaa ttgattcatt caattggatg tttggaggaa tagggaatga aagaaaaaga
     6361 gtttttacaa gaagtaaaag aaatcaatct tctacaacaa gctttaggaa tcctcgattg
     6421 ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag tagacagcta
     6481 tctttacagc atttattttt caaaaaaaat cggtccaaaa atcaaagaag ccattcgcta
     6541 tttttctgaa catccggaag aattgtcgga agttggaact gccgtttttg ccaaagtaaa
     6601 agaagagtat gatttggaac atcgcgtacc ggaagatctt atgagaaaat atacagctgc
     6661 cgtttcttca gcacacggac aatggcaaaa agctagagaa acacaacaat tttctgattt
     6721 tcaacacgca ttgaatcaaa atattgaatt aacaaaacag ttgatacctt actggagaaa
     6781 agatgaaaag acgaattatg atgttttgct gaatcaatac gaacccggga tgactgtcga
     6841 aatccttgat aaggtatttg atcagcttaa agaaggaatt ctttcgatcc gccgtacgct
     6901 ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat cggagaatga cgaaaaaaca
     6961 acagaagaga tttgtagtga aagtaatcga acaacttggt tatgatttta gtcgtggtcg
     7021 tttagatgac actgtccacc catttatgac tggaattaat ccgaatgatg cacgaatcac
     7081 gacacgatgg aacgaaacag atttcaaaat ggctgttttt ggagtgatcc acgaggctgg
     7141 tcatggtatg tatgaacaag acatcgatga taaatatgcg tatacaccgg tttatgaagg
     7201 tacatcaatg ggtatccatg aatcacaatc gttgtttaac gagatcatta ttggaagtag
     7261 tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag gaacattcga
     7321 tgatattcct tttgacaaat tttatcgttc attgaaccat acaaaatcaa gtttggttcg
     7381 tatcgaggca gacagtttga cttaccctct ccatatcatc attcgttatg aaatcgaaaa
     7441 aatgattttc aatgaagggg tagatgttgc tgaactacct gaaatatgga atcaaaaata
     7501 tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac aagacgttca
     7561 ttggtcaggt gcaagctttg gttattttcc ctcttatgca ttagggtaca tgtatgcggc
     7621 acaattactt cattctttgc agcaagaaat agatgtggaa aatgttctag cgtcagacga
     7681 ttattctcca atcaaaaact ggatgtcaga gcatatccat caatatggtg catctagaaa
     7741 accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat acctgatcga
     7801 ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa tttacaaaga
     7861 tagctgaaat tcatctaatc tatagatgtt ttcagctatt tacttaagaa agaagtggaa
     7921 cgaatggata aattacaagt gatcgctgat tatagtatgg accaattagc gcatgatcaa
     7981 acaggacatg ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa aattttggag
     8041 acggaaccgc aggcagaccg ctttgtgacg cttgcagcag cttacctaca tgatacgatt
     8101 gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt attccttcgc
     8161 tctttgccaa tagtagagga acagatctcg atgatttttt ccattatcga aaatatgtcc
     8221 ttttctaaaa atctctctga agcggccgaa ctatctctgg aaggtaagat cgttcaagac
     8281 gctgatcgta tcgaagcatt aggagcaatc ggcattttgc gtactgctta tttcggcggt
     8341 ggacatgggc acccgatttt tgatcctgaa ctgtatccgc aaacattcaa ggacaaagaa
     8401 aattaccgaa aaggcacgac agtgatcaat catttttatg aaaaactgtt cttgctgtct
     8461 gataaaatga atactgatta cggatatgaa gaagccaaac gaagagaaac cttcatgcgt
     8521 gaattcttag aagaattttt tctggaatgg tctattgacg atgaaaattt tactccagac
     8581 aattggatca aaaataacaa aaaatgatgg aggttgtgac agaagtggtc agctccaaga
     8641 tatttcggat cattttccgt aaaagttact tctggaacac tgtttaccaa gaggtcgtga
     8701 gcctgccgtt cagctccgag tcacaaggaa caatttcaaa aaatagcttc tcgtgtttga
     8761 tgaatattgt aacttgtgat tcggaattgg caggagaaca ccgtttattc ggaagaatag
     8821 gactgtgaca agacttttgt cacagtcctt ttttcgaatt caacaagtaa aacttttaag
     8881 aaatattcag agagtaaaat taaaaattgc tgataaacga agatggaaat tttatgtatt
     8941 tttattggtc tatgcaataa ataaattaaa tattcagaat acaattgatt ttaacgaaag
     9001 ggcttctatc aatggttgtg ttgtatttat ctgcccaggt agagtaaaat agatcattat
     9061 ctaaaaggta attgttcata ctatataagt catttgaaag taaaaagata ctgtaaataa
     9121 aaaatgtggt taaataattc tcataatcag tgtaagcgtt atcaaaaata tagcatactt
     9181 gctttgtaga tgaatgacac gatatatgtt tggtatgttc tgcaagacag atgaatatgg
     9241 aggtggagac aatgtcttcc aaagaactaa caatgtattt agagaaagaa ttggatcaac
     9301 tgaaagaaaa ggggttatac aatacgatcg acatactaga aagtgaaaat ggtgcttgta
     9361 tcatcgtaga cggaaaaaag atgatcaatc tagcatccaa caattatctt ggatttgcca
     9421 atcgcgaaga attgaaaaaa gcatgtattg aagcaaccga aacttatggt gtaggagcag
     9481 gagcagtccg aacgatcaat ggttcgctga aaatccatca gcaattagaa gaaaaaatcg
     9541 cagaattcaa agggacagaa gcagcaatcg cctttcaatc tggattcaac tgcaatatgg
     9601 gcgctatctc tgcagtaatg acaaaagaag atgctatctt atcggatgaa ttaaaccatg
     9661 catctattat tgacggttgc cgcttatccg gagcaaaaat catccgaatt aaacatcaag
     9721 atatgaaaga ccttgagaaa aaagcaaagg aagcgataga aagtaagaaa tataaaaaaa
     9781 tcatgtatat cacagatggt gttttctcca tggatggaga cattgcacga ttgccggaaa
     9841 ttattccgat tgttgaaaaa tatggactga tcacctacgt cgatgatgcg cacggctcag
     9901 gcgtaactgg aaaaggtgca ggaaccgtca agcattttgg attaagcgac aaaatcgata
     9961 tgcaaatggg cacgttatcg aaagctatag gtgttgttgg gggttatgta gctggatcga
    10021 aaacactaat cgactggttg aaagctagaa gtcggccatt cctattttct acttcgctaa
    10081 ctccaggtgc agctgcagct gcattggctt cgattacttt gatgcaagaa catccggaac
    10141 ttgtcgaaaa agtttgggaa aatgctaatt attttaaaga agaattgaaa aaagtaggct
    10201 ataacatcgg tatgtctgaa acacctatta ctccagtcat tttgggagac gaaaaagtga
    10261 ctcagacttt ctctaagaag ctgatcgaac atgggattta tgctaaacca atcgtttatc
    10321 caactgtgcc tcttgggaca ggcaggatca gaaatatgcc gactgcggaa catacgaggg
    10381 aaatgcttga tgaagcagta gcagtttatc aaaaaatagg aaaagaaatg gaaattattt
    10441 aactaaattt agttttttcg tctatttaaa attagagaac atgttcacgc atatattgtt
    10501 aatcatctga agatagtagt aaaaaagatc agggaggatc atcatgaaaa aagtattggt
    10561 gacaggatgt ttaggacaaa ttggttcaga gttgatcaca cggctacgtc aagaactagg
    10621 cagtgaaaat gtcattgcga cagatatacg catgcctgaa gtcattgaga aaaacgatat
    10681 ctttgagatc ctagatgtaa tggattacga aaaaatgcgc agcatagcag aacaatatca
    10741 agtggacaca ttgattcatt tggctgctct tttgtcagct gttgcagaag caaaaccaca
    10801 attagcctgg aatttgaacg tgactgggct ggtcaatgca ttggaagtcg ctagagaatt
    10861 ccagctgaaa ttttttacgc caagttcaat cggcgcgttt ggtccaaata ctccaaaaga
    10921 tcatacccca caagatactc ttcagcgtcc tacaaccatg tatggggtaa cgaaggtagc
    10981 tggagaacta ctatgtgatt attattatgt gaaatatggc gtagatactc gtggtgtccg
    11041 tttccctgga ttgatttctt atgcaacctt gccaggaggc ggtacgacag actacgcagt
    11101 cgatatttat tattcagcaa tcaaagaagg aaagtacact agtttcattg atcaaggtac
    11161 atcaatggat atgatgtata tgccagatgc gatcgatgcc attataaaac tcatgaatgc
    11221 agatcctgat cgattggttc atcgaaatgc cttcaatata tcggcgatgt cttttgaccc
    11281 ggaagagatc aaagcatcta ttcaaaaaat cataccagat tttgaaatgg attatgatgt
    11341 ggatcctgta cgtcagtcaa tagcagaatc ttggccaaat tcactagatg tttcttgtgc
    11401 gcaagaggaa tggggctttt cgccgaaata cgacttagat gtgatgacaa aggatatgtt
    11461 ggaaaaactg aaagaaaaat tggtagagac ggtataaaac aagaaccaca tccaaaaagt
    11521 tttttatact tttgggattg gctatggtat gaaaggggac aagggatgga agaatatcaa
    11581 gccacgccag taaaagaagt aaaaatagaa aatgaattaa aacgtacgat gggatttttt
    11641 actgctttat cgactgttat gggcacagtg atcggtgcag gggtattttt taaagccgca
    11701 agcgtagcag aagttacagg atccgccagt ttgcatatgt tttcttggtt tctcggtggt
    11761 atgatttctg tctgtgcagg actaacagga gctgaactgg cagctgctat tccagaaaca
    11821 ggagggatga ttaaatatat cgaacggatt tacggaaata cagcagcttt tttattgggc
    11881 tgggcgcaag tcgtgattta ttttccagca aatgtagcag ccttgtcgat cattttcggt
    11941 acacagttcg tcaatctatt tggtctttcc cagtcaatga ttgttcccgt agctgttaca
    12001 gctgctgtat cgatcctgtt gatcaatttt ttaggttcta aagctggagg ggcttttcag
    12061 tcgattacgc ttgtctgcaa gcttattcct ttgtttgtga tcgtcatttt cggtttgttc
    12121 cgacaagaag gagtagactt ccaactgttt ccgatccaag caggagaaaa tctttcattt
    12181 ttctcagcct tgggagcggg cttgctagca acgatgttcg catacgacgg ttggatacac
    12241 gtaggaaata tttcgggaga attgaaaaag ccggcgaaag atttgccaaa agcaatttct
    12301 ttaggaatca tcggaatcat gatcgtttat ttattagtaa atgcggtgtt cttaagaacc
    12361 gcatctatcg acggagtcgc aggaaacagc aatgcggcaa gtgacgttgc aaaaatgatt
    12421 tttggcggct tcggtggaag actggtcaca gttggtatct tgatttctgt ttatggaacc
    12481 atcaatggct atacccttac tggaatgcgt ctcccttatg ttatggcaaa agaaaacaac
    12541 ctgcctttta gcaagctctt tgctaaactt catgataaaa ctaaagttcc tgtagccgca
    12601 ggtatcttag agctagtcat cgctattggc atgatgatga tcggtggttt tgatacacta
    12661 acggatatgc tgatctttgt catttggatt ttttatacga tggtctttgt tggtgtcatt
    12721 cttcttcgta aaaaagaacc tgatttgttt cgaccataca aagttccaat gtatccgttt
    12781 attccactag tagcaatcat tggagggact tttatcgtat caagcacgct tatcactcag
    12841 acctttttag cttcgatggg gatagtattg acacttgctg gtatcccgat ttatctctat
    12901 ttaaaacgta gatagctaaa acattcgaat tttattagaa ctgcacttct ctgccaaaca
    12961 aaatatttta tttaaatgag gagtacgtgg aagaaatgtc aaaagtactc gttaattttg
    13021 atcacgtaaa tgtcgttttg tctacgatga aatagcaaag taaaagacag aaatcgggct
    13081 caaccgattt ctgtctttta ctttgatttg cgtaactgaa ctattcggcg tacgccgcga
    13141 taaatattta ataaaaccaa gcaaccagaa atgaaaatgg gaagccaaat cagatagaca
    13201 gggtagccaa gcactttaga caaagcatgt ggagtcattt tatccatttc aagaagtgct
    13261 tgctgccaag tctgaagatt gaagactagc atgaaacccg aaataatcag gagaataaat
    13321 cctaaataat gaattgtagt tctttttctt acataagcga tcgtagcata aaggaacagt
    13381 aaaaaaatag gtacgccaaa aacaaagtac atattcagca tctcgacacc ccctttgtgt
    13441 ttatcttact ttactttgaa agaaatgaga aataatagcc tcgttacttg cttgaaacag
    13501 caaaggactg ctaagataag agagatacaa agaatcaagg aggagtcata tggcaagtta
    13561 tatcgaaccg attcgttttg cgctgattgt ttttccattt ttagccttag cgatctcatc
    13621 tgtttttttt atttatgaat atcgaaagta tggaacattt ctagtaacaa gggcagttat
    13681 tttgtattct ttcgtttttt atttattgtg tgcgtatttt ttggtgatct taccacttcc
    13741 ggcaaaggag acggttgccc aaatgacagg gccaaagatg gagctgcatt tatttgcttc
    13801 atggcaacat ttcagagaca acacggtatt ggtcttgtct gaacctagta cctacctgcc
    13861 ggcaatgaag cagagtgtat ttttggaacc agcttttaat gttttattac tcataccatt
    13921 tggcatttat ttaagatatt atttccggct ttcttttatg aaaacgatga ttgctagttt
    13981 ctgcctttct ctattttttg aattgacgca gctaagtgga ctttatttta tctacccaag
    14041 accataccgc ttatttgatg tgaatgattt agtgacaaat acattgggtg gaatcattgg
    14101 ttttgtcgtt acgccgttat ttacatttat gcttcctaca agaagacgaa tggatgaact
    14161 ttcttatgaa aaaggaaaga ctgtctcatt gactcgacgc ttggtcgctt atttgatcga
    14221 ctggacattt ttatctctta ttaatttggt agcggcgatt ttctttaaaa ttgcgttcga
    14281 ttcaggggca gttacttctc atatctggtg gcttttatta gaagtagggg tatactttat
    14341 gattgtaccg tttataacca atggatggac acttggcaaa aaaattgtgc gtatccgttt
    14401 ggtggaagaa gaccgcgaac gaatttcttt aaaagcattg atgatccgct acgggtatct
    14461 gtactttctt ttttatggtt taagctggtt ggggcagttg cttcagcaaa tcatgaattc
    14521 gacaacgaat acggtgttgc agatgagtgc agtattcatt tctctcggtg tcttgttact
    14581 acaaggtatt tttattttaa atatcttgct atcatttttg agaaaaaaac ggattctgtt
    14641 ttatgaaaaa gctagtcaca catatactgt tagcacgatt ggccaaacaa aggatgatga
    14701 ttctcgaaat tagctgagca aaaacgcttt gtctgtgtta taatgccaaa gtcgtgataa
    14761 tttaaagaga agaggtgcac gaatgaataa ttttgatata ttccgcttca aaccgtttat
    14821 caatgaagcg ttaaccgcaa agggcttcaa acagcccaca gaggtccagg aacgtttgat
    14881 tcctttaatt gcaaaaggaa aaaatatcat tgggcaatct caaactggta caggaaaaac
    14941 acatacgttt ttgttgcctt tgtttgaaaa aatcaatgca gaaaaagagg aagtacaagt
    15001 tgttattaca gctccaagta gggaactagc tgcacagatc tatgaagcag ctacacaaat
    15061 cgctaaattt tctacaccgg agatccgtgt ctcgaatttt gtcggcggta cagacaaaca
    15121 gcgtcaatta aatcgtctgc atcaccaaca gccacaggtt gtagtaggca cgcctggtag
    15181 aatattagac atgatgaatg aacaagcgct gaagattcac accgcatttg catttgttgt
    15241 cgatgaagcg gatatgacat tggatatggg ttttttaaat gaagtagacc aaattgcaag
    15301 tcgattacca gaaaagctgc agtttttagt tttttctgcc actattccag aaaaacttcg
    15361 tccattctta cgaaaataca tggaaaatcc aatcattgaa gaaatcaaac caaaagcagt
    15421 tatttctgaa gacattgaaa attggctgat ttcaacaaaa ggtaaagatg agaatcaaat
    15481 gatttatcaa ctgctgacaa ttggtcatcc ttatttagca atcgtatttg ccaatacaaa
    15541 acagcatgtg gatgaaattg ccgattactt gaaaaaccaa ggcttgaaag tagcaaagat
    15601 tcatggagac atcaccccaa gagagagaaa acgagtgatg cgacaagtac agaatctgga
    15661 gtatcaatat gttgtagcga ctgatttagc tgctagaggg atcgatatcg aaggtgtctc
    15721 tcatgttatt aatgcagaaa ttccagaaga tctagacttc tttatccatc gtgtcggacg
    15781 tactggacgt aatggcttaa aaggaacggc cattacgtta tattcgccaa atgatgagca
    15841 agccattgat gcgatagaaa aattgggcgt ctcatttgaa ccaaaagaaa tcaaaaacgg
    15901 ggaaatcgtc aaaacatacg atcgtaatag acgtgccaaa cgagaaaaat caaaagatgt
    15961 attagatccc acgctgattg gtcttgtgaa gaagaaaaag aaaaagatca agcctggtta
    16021 taagaaaaaa atcgattggg caattgcaga aaaaaataaa aaagaacgca aaatcgaacg
    16081 cagacagcaa agccgtactg ctcgtaaaaa taagaaaaat tcacataaat aaaaaacaag
    16141 aggttgtgac aaaaggcgtg tcacaatctc ttgtttttaa gctagacttt ttttcattcc
    16201 gatacaaagt acttcatctt caataaaagg ttctgtgcaa gtatgatacc cttgtttttc
    16261 ataaaaatct acagccgtca tttcagctac tacataacta gtctgacaac cttctgaaac
    16321 cgcccgtttt tcagcagctt caagcaactg accgcctaac ccttttcctc ggtattcttt
    16381 tgctacgcaa aatcgttccg gcttgaagca agtaggtgtt ttcttttcat aacgcaaagt
    16441 agctactggt cttttttgat caagaaatag ataataggtt tgttgcggga tatcttttcc
    16501 gtcgaattct agttctgcag gaatcccttg ttctaataca aaaacttgat atctcaaaaa
    16561 gcagctagct gcgcgtatcc attcatccgc tccaactatt tttttcatca tattcctcct
    16621 tgtcatgaat agcagagatg tattgattcc tttattctta tcgtaccaaa aatcgtggag
    16681 tatgaccaaa aggaaatgaa aataaaaatt gagatattga cagtgatcaa tagaatgcgt
    16741 atactaatta agtacgaaaa ggatatgatt tgttttttaa gactcacaga aaattctttc
    16801 ttggctgaaa aaagaatagt cactaatcaa attgctccct ttttctattt tggtagaaat
    16861 accgacgcag ctcagcgtta tcgagtttaa gaggtaatga ttacacatca ttgcaatcaa
    16921 ggtggtaccg cgagttttcg tccttggctg tgatggtgtt tttttgtatt taatttatag
    16981 aaagcaggta atgacatgaa acaattaagt agtgcacaag ttcgacaaat gtttcttgat
    17041 ttcttccaat ctaaaggaca tacgattgaa cctagtgcgt ctttgatccc cgtgaacgat
    17101 ccaaccttat tgtggatcaa ctctggtgtt gcaacattga aaaaatattt tgatggttca
    17161 gttgtgccag aaaatccaag aatcacaaat gcccaaaaat ctattcgaac aaatgatatc
    17221 gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg agatgctggg taatttttcg
    17281 attggtgact atttcaaaaa tgaagccatc cactgggcat gggaattttt gactgatcca
    17341 aaatggatgg cattcgatcc agaaaaactt tatgtaacag tttatccaaa agatacagaa
    17401 gcaaaacgca tctggcatga agaagttggc ctgccactag atcacattat tgatgtagaa
    17461 gacaacttct gggatatcgg tgcaggccca tgtggtccgg atacggaaat tttctatgac
    17521 cgtggagaag cttataacga tttagcagaa gacgatcctg aaaactatcc aggaggagaa
    17581 aatgaacgtt atttagaaat ctggaactta gtattttcag aattcaacca taagcctgat
    17641 aacacatttg aaccacttcc tcataaaaac attgatacgg ggatgggatt ggaacgcatg
    17701 gtctcaatcg ttcaagatgc gtcaacgaac tttgagacag acttgtttat gcctattatc
    17761 cacgccgtag aaaagctaag caaacaagta aaatatggtg aaaacccagt aacagatatt
    17821 tcttacaaag taatcgcaga tcatattcgc gccctttcat ttgctattgg cgacggtgca
    17881 ttgccatcga atgaagggag agggtatgtc ttacgccgtt tgcttcgtcg tgctgtcatg
    17941 catgggaaaa aattaggtat tgaacaagca ttcttatatc gtttagtccc tgttgttggg
    18001 gaaatcatgg taagttatta tcctgaagta cttcaacaaa aagaatttat tgaaaaagtt
    18061 atccgtacag aagaagaacg tttcaatgaa acaatcaatg aaggtttgga aatcttaaat
    18121 caagtgatcc aagaagtaaa aaacgaaaac gggacgactc tagcaggaaa agacatcttc
    18181 aaactatacg acacatatgg cttcccggta gaattaacgg aagaggtggc agaagatgaa
    18241 ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg atgcacaacg cgaacgtgct
    18301 cgctctgccc gcagtactga aaaatcaatg ggggtacaat ctgctttatt gacagatatc
    18361 aaagtagaaa gtaaattcat tggatacaca gaacaagaat cagatagtca attattggtc
    18421 atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag gtgaagctca aatgatcttt
    18481 gatcaaacac ctttctatgc agaaatgggt gggcaagtag cggatcaagg atggatcatg
    18541 aatgaagtag gcgaaatcgt agcagttgtt acagatgttc aaaaagcgcc taatggtcaa
    18601 tttatgcaca ccgtagaagt aaaagcacca atgagtgaag gacaaaccta tcatctattg
    18661 atcgatcaat cgatgcgcaa tcggatcatc aaaaaccata cagcaaccca tctactgcac
    18721 aaggcattga aagaagtact tggcgagcat gcaaatcaag ctggttcttt agtcgctcct
    18781 ggacatttac gattcgactt tacccacttt ggtcaaatca cttcagatga attagctgaa
    18841 atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc cagttgaaac aattgaaaca
    18901 gatatcgata cagccaaaaa catgggtgca atggctttgt ttggtgaaaa atacggacat
    18961 gacgttcgag tagtaaatat tggcggctgg tcaatcgaac tgtgcggagg cacacatgta
    19021 gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg aatcaggaat tggtgcaggc
    19081 gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg aactacttga agaagaagaa
    19141 aaacaactaa aacaagtagc aacaattgtt aaatcacctc aattgaaaga agttgtttca
    19201 aaagcggaac aattgcaaca gcaagtaaga gagctacaaa aggaaaatga acaattagct
    19261 agcaagttag cgaatcaaca agctggtgat gtattcaaag atccagaaac gatcaatgga
    19321 aaaacaatca ttgcagcaca agtaaaagtg aaagatatga atcagctgcg tcaattagct
    19381 gaccaatgga aacaaaaaaa tatttcagat attcttgttt tagcaaacga acaagaagga
    19441 aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg aagctggact aaaagctggc
    19501 gacttgatta aagcaatcgc accgaaagta ggcggtggcg gtggtggacg tccagacatg
    19561 gcacaagctg gcgggaaaaa tcctgctggt attccagatg cacttaaagc tgcgaaagaa
    19621 tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa acaaaagttc aaacattgaa
    19681 cgaaacatga ttatgggggc gaagacaaac aagtcttgcc ctcatacttt tttcaattgg
    19741 atggcgcatc taatcaaatg gtcggattcg gatggtcttt agttgacgaa agtgacagaa
    19801 atttttacga ttagtataaa taaaaagggg tgaaaaaatg aatagtagaa ataagacctt
    19861 tcgactagtc ttacgtgcga tcttgttagc aatcatcatc gtacaagcga tggttccttg
    19921 gctggggttt attccattag gattcattag tttaacaatt atccatatca cagtaattat
    19981 tgctgcagta gtattaggcc caaaagatgg catggtgatt ggcttgtttt ggggaatcgc
    20041 tacaattgtt cgtgcctatg ccatgccaac aacgccattt gatacattag tgttcacgaa
    20101 tccgattatc tcggtagttc ccagagtact tgttggctta gtagcaggct tggttttcca
    20161 ctggatttat caaaaaaata aatcaataac gatcagctca gcctttgcag gggtactggg
    20221 ttcattggtc aatacagtct tagtgttagg attcatggga ttattttata caggtgcaac
    20281 ggcagatgcc tatggtgtag actctgctct tttatttaaa accttagcag gggttgcagc
    20341 aatcaatggg ataccagaag caataggtgc gggaatcata acaccattac tagcaaaagc
    20401 gttatttgca gcaacaccgt tgaaaccaga ataatgacca tcttttaata gtaataggac
    20461 gcacgataga tagaaaaagg aagagaggag aggaaaacaa tcgtcctctc catcttttta
    20521 cctgagcaac aagcaaacta gaaggggtaa aaaacttggc taagaaacaa gaagaaaaaa
    20581 agaaacaaat accgcaacag cggacaaaaa aaggagtact agcgggtatc ttaggattgg
    20641 ttacgaatct gatcttgttt gtctctaagc taatgatcgg gcttttttca ggaagtgtct
    20701 ccattatggc tgatgcagtg aacagtctat cagatactgc ttcatccata ttgactttga
    20761 tcggatttaa aattgctgca aagccagcag atcaggaaca tccttatgga cacgaacgct
    20821 tcgaatatat cagtgggtta tttgtttcga tcatcattac ttatgtagga tttcaatttt
    20881 tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt ctctttaaca cccatcgtct
    20941 tttttgtcct aatcatttcc atctttttga aatttgcaca aggcaaaatg tatcaggtta
    21001 ttgcgcaatc cattgattct gaaacattga gagctacagc aacagatagt tataatgatg
    21061 tctttactac actggctgta ttgctttcag cagcaatcga atgggtaact ggatggcgta
    21121 ttgatggata tataggtttt gctttagcat tatatatcct ttatagtgga ttgacgatgc
    21181 ttcgaaattt tgtatacgaa ctattaggaa gtcgcccaac gccagcagag attgaagaaa
    21241 tggaagagca tttaaatgct tgcgatgata tacttggcta tcatgattta ttggtgcata
    21301 actatggacc caataaacgc tttgcatcag tccacatcga agtagatgaa tcaatgaatt
    21361 tgaatgtggc tcataagatt attgatctga tagaaaaaga ctttaaaaag tctttggatg
    21421 tggaacttgt ctgtcacttg gatccaatgc ctgttgataa cgaaaaatac tataatatct
    21481 tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg gcttaatatt catgatttta
    21541 gagtaattca tcaaggaaaa acgctgcaat ttgatgtagt ggtccctgag aaattccagt
    21601 ttgttgatac agaactagaa agaatcttac gtagtcaaat agaagaaaaa atcggagatt
    21661 accaattgga aattacgttt gatcacaatt atcttttata ttaaatgaga aactataaaa
    21721 agactcctta aagtttttaa tttaaagagt ctttttttct tttaattgtt tatggagagc
    21781 ctgacgtgca agattatctg ctcctttatt tttgttttcg ggcaaccatt gcaggatcaa
    21841 taaagaaaat tcggatagaa gttcttggat tctttgaagg tagggaagga agagctcgtt
    21901 cttcgttgcg tttttgtcga ccgtagaaac tagtgttttg ctgtctgaaa aacagaaaat
    21961 cgtttcattc tgtaagtcgt ttttctttag aaattctaaa gtttttaaaa agacttcgaa
    22021 ctctgcttga tggtttgtcc ctatagaaag cgggatagat agctgttctt gtttaccttg
    22081 atagacaatc aagattccac ctccactagg accagggttg ccttttgtag aggcatcaat
    22141 gtatactttt aacataaaaa gagattctcc tttttgttca aactaatgtt atggtaagat
    22201 tatatcatta ctgaatcggt gagaggttag agaatgaaaa aaaatattcg atggcagcct
    22261 gaactggcac atacgatcat ctattggtca ttgaccttga tcattctgtt ttttagtctg
    22321 attctttctt tagaaaacac aggtccttat tggaaaagca attttgtttt aggtattttt
    22381 cttgtttttc tatttctagg tttgcggcgt tcttttattt taaaatcaaa ctctgtgatt
    22441 atacgttatg ctgctttttg gaaagaccga gaaatatctg tcggacagat tcgatctttg
    22501 acgccaacta atactggggt gcaaatgatg ttgcaagatg agaaagcccc atctgttttc
    22561 atgatgcgaa aaaaagatca agtagtcctt tttgatcatt ttgaaaaaaa cggacccaaa
    22621 tcagaatcaa ttccttatcc ttatacgaaa aaaagaaaaa gctaatttta aaaatcagaa
    22681 cctaaaaaaa cgaattttta caggaaaaga aaagagtgag acgtacggtt catcccgttt
    22741 agcgtctcac tctatttgtt tatctattca tgcatactag tatcggtagt ctgaacgttg
    22801 cctttcttga catgggcagc ttgtgttcct cgattccctt cgatgatctc aaattcgacc
    22861 acttgatttt cttccaaagt tttgaatccc tcttcttcaa tcgcagtaaa atgtacaaaa
    22921 atttcttctg tgtcatcata actgataaag ccgtatcctt ttttgttgtc aaaccatttt
    22981 acaattcctg tggtcaaagc actcacgacc tttctaaagt ctgatcatag ccttcattat
    23041 aaagagtatt tataagtacg tcaaataaat taaaaacctg taattttatg ttttttttct
    23101 gaataaaaaa aggtataatt ggcatatctt aacaaagaag atattttgtt gaataaagtt
    23161 cgtgaaaagg ggtaatcaaa tgaagaatga tctgcaaatt tcacaagaaa cagagctaga
    23221 accgattgtt gaagtggcta gtaaattaga tcttaaggaa gacgatttgg aactgtatgg
    23281 gaaatataag gcgaaaatca atttttctgg aatcaatcga tcaaaggata acgctgaggg
    23341 gcatttgatc ttagttactt ccatcaatcc aacaccagct ggcgaaggaa agtcaacgat
    23401 cactgtaggg ttaggagacg cgctcaataa aatcaataaa aaatctgtta ttgcattgcg
    23461 agaaccctca cttggtcctg tgatgggaat caaaggtggt gctgcaggcg gaggatatgc
    23521 acaggtattg cctatggaag atatcaactt gcattttact ggtgacatgc atgctatcac
    23581 aacagctaat aatgcattat ctgctctttt agataatcat attcatcaag gaaatgaatt
    23641 gaacatcgat gcccgccgag tgatttggaa acgggtagtc gatctcaatg atcgtgaact
    23701 gcgtaaagta attgttggct tgggtgggcc aatccaaggt gttccaagag aagatgggtt
    23761 tgatatcaca gttgccagcg aaatcatggc tatcttgtgt cttgcggcag accttgaaga
    23821 tttaaaggct cgtcttgcaa ggatcgttgt cggctataca tttgaccgtc agcctgtgac
    23881 tgctggagac ttgaaagcag aaggcgctct ggcattactg ctgaaagatg caatcaagcc
    23941 gaatcttgta caaacaattt acggaacacc agcttttgtc cacgggggac cgtttgccaa
    24001 tatcgctcat ggctgcaata gtattttagc taccaagaca gcattgagat tagcagatta
    24061 cgttgtcaca gaggcaggat ttggtgcaga tcttggcgga gagaaatttt tagatatcaa
    24121 agtacctaac ttgaaaaaag cgccggatgc tgtggtgatt gtcgccactg tccgtgcatt
    24181 gaaaatgcat ggcggaatga aaaaagatga acttaaaaat gagaatcttg atgctttgaa
    24241 agtaggtttt gcaaatttaa agcgccatat acgtaatatg gaacaatatc agctacctgt
    24301 gatcgtcgca atcaatgaat tcgtaacaga tacagacaat gaactcacac tgttggagca
    24361 cttgtgtgaa aatcagggga ttcttgccaa gagagcgagt gtatgggcag atggagcaga
    24421 aggcggcgtt gatttagcaa aagctgtagt acgattgatt gatagaaaag aagctaatta
    24481 caagccatta taccgcctag aagaaacgat acaagaaaag acagaaacaa tcgtcaagaa
    24541 aatttacgga ggaaatggtg ttgttttcag caaaaaagcg caaaaacaaa tagaagagtt
    24601 tacgaagaat ggatgggaca aactgccgat ttgtatggca aaaacacagt attcattttc
    24661 tgatgatcct tcactgctag gtgcaccgga agatttcacc atcacgatcc gcgaaatcat
    24721 cccaaaatta ggggcaggat ttttagtagc cttgactgga gatgttatga cgatgcctgg
    24781 tttgcctaaa aaacctgctg cattgaatat ggacgtgaca aatgacggag aagtgttggg
    24841 attgttttaa gcttgattat aatctaccat cgaaggagct gtattttgac tacagcttct
    24901 ttttttagac gatagggaat taattttgtt tacactagga tcattctttt tctttattgt
    24961 attttgagct atactaaagc ataataggag ggtatgatga aaacgacatt tataagaaaa
    25021 accggttttt acggtatggg tagctcgatt cagctaagaa tcaacaatga aaaacgcagt
    25081 gttctcagtc atgaacaaaa gataacgctt gatctggaag tgccctttac gatgcaggtc
    25141 accttttatt ggctaaaaag ccccgtttat acagtgaaag aagcaaagcc tgcttatgtg
    25201 atcacaatga attacttgat tcttcaaatc tatccgtttt tatttctagc aactgggttg
    25261 ggggccgttt tcctacaaaa tttatttttc agtttttttg cagttattgt gttgattgcg
    25321 ttctttttct atataaaaaa ccgggcatat ttgatcaagg aggaaaatta tggcaaactc
    25381 tgatcttttt ttaatcagtt tcaatcgaat cgaaaaatgg atgagagacg agatgggaaa
    25441 tgcacgaaat atgggattta ccgaacttgt cagacgccta gctcaaagaa aacaattaat
    25501 gatcagaaaa tacgaagatg atcttttaca gcttgctcag ttaaggaatg cgatcgtcca
    25561 tgatcggatc gcagtcgatt ttgtcattgc agagccaaat gattggacag tcaagcgaat
    25621 ccagctgatc gaacaagaac tgattcagcc tgaaaccgtg ctgcctcgat ttgcaaaacg
    25681 tgtaacaggt tttgaacaag atcttccgtt acttgaactg ttaaagatag tagcagaaaa
    25741 aaggtattca caatttccgt tatacaataa aggaaaattt gtagcattga ttactttacg
    25801 taatatcggg ttttggctag caaaagaaag ccaaaaaggt ccggttgatt taacaaataa
    25861 acgagcgttg gatttgatca tccaaaacgg aaagtacaca aattatcatt ttgttccagc
    25921 taaaacacat atttacgagg ttgaagcgat gtttcgtgag caagggactt tggaagccgt
    25981 acttatcact aaagatggga atcccgatgg taatctgctc gggatcgtac gacctcgtga
    26041 tatttataaa caaatagaaa aggattgatt ttttgttaat tatttattgg atattcagcg
    26101 ctttgctagt aggagtggat cagttggtca aatggtggat cactgataat ctagcgcttg
    26161 gagagacgaa aaatcttata ccaggtattt tgtcactgaa tcatattcga aacactggag
    26221 ccgcatggag tatgctagaa gggaagatgt ggttctttac tatagtgaca ctcattgcag
    26281 tcgttgtgat tctcacattg atgattaaaa atcgtgaaaa aggtaatcgc tggttcatga
    26341 ttggattgag tttgattcta gctggtgcga ttggcaattt tatcgaccgt attcgtcttg
    26401 gctatgtagt agatatgttc cagacagatt ttatgagctt tccgattttt aacgtagcag
    26461 atgtgacact tgtatgtgga gtgatttgta tgttaattta tattatttta gatgaaaagg
    26521 atcaaagaaa aaaatgacag aacttaaaac gacgattcac catgaatctg gtaggatcga
    26581 taaagtattg actaatttat tcaccgatta cagtcgttcg caaattcagc tgtggctgaa
    26641 aaatggagcg gtttctgtca atggacagcc agtcaaagca aattacaaag taaaacaagg
    26701 agacgagatt actgtcttga tccccgaacc agaaacatta gatgtggtcc cagaaaatat
    26761 cccgttgacg atcgtttacg aagatgaaga tgtagcggtg ataaacaaac ctcaaggaat
    26821 ggtcgttcat ccttcagcag gtcatacgca tggcacaatg gtcaatgcgc tgatgtacca
    26881 tatcaaggat ctttctacga tcaacggcgt tattcgtcca ggtattgttc accgaatcga
    26941 caaagatact tccggactgc taatggttgc caaaaatgac caggcacacg aatcattagc
    27001 tcagcaatta aaagataaga cctctttgcg caaatatgtt gctttagtcc atggagagat
    27061 tcctcacgaa aaaggaacca tcaatgcgcc aatcggccgt tcgaaggaaa accggaaaat
    27121 gcaggcgata agagaagatg ggaagtcggc tgtcacacat tttactgtat tggaacgttt
    27181 cgaaggattt actctggttg aattaaaact agaaaccgga agaacacacc agatccgtgt
    27241 gcatatgaag tatatcggtt atccgttagc tggagatcca gtatacggac cgaagaaaac
    27301 tttgaaaggc aatggtcagt ttctccatgc gcaaatgtta ggttttacgc acccgactac
    27361 aaacgaaaag atgatctttg aagcacctct tccagaagtt tttgaaaaaa ctttagaaaa
    27421 attaagaaaa aacattgcat tttaatttag ataacggtat agtaattaac aacgaaagca
    27481 taaaacttaa tagaattcct ttaagtttag tcccgtgagg ctaagaaggg tgggagcaag
    27541 gatacgtatg gtgcgccata agtagattgc tcattgacat agtatagcta tcagtgtatc
    27601 agtatacgct cagtctaaac attcctcctg tcctcttagg gcaggagttt ttttatgcaa
    27661 aaaagtaggg ggaatgaaaa tgcaagcaaa agaagtagta gatcaggtaa ccatgaagcg
    27721 agcattgact cgaattactt atgagattat cgagagaaac catagtatcc aagatatcgt
    27781 attggtaggt atcaaaacac gaggaatcta cattgcatca agaattgccg aacgcctgaa
    27841 gcagctagaa gatatcgata ttcctgtagg agaactggat atcactttat accgagatga
    27901 caaaaaagaa aatccagaag aaccggaact tcattcttct gacatcccag tttccttaga
    27961 aggaaaagaa gttattttga ttgatgatgt tttgtataca ggacgaacga ttcgagcagc
    28021 aatggacgca gtaatggatt ttggcagacc aagaaaaatt tcattagcag tcctggtcga
    28081 tcgtgggcat cgagaattgc caatccgtgc agattatgtt gggaagaaca ttccaacatc
    28141 tcgggcggaa gagattttag tagagatgca agagcttgat ggacaagatc gcatcatgat
    28201 tttgaaagag gaagattaga ggagagagaa acatgtcgga gaaaaaagga tttcataatg
    28261 atgacgtggt gttagatatc cacgatcgcc caaccccagg acattgggta ggattaagtt
    28321 tacaacactt gttcacgatg ttcggagcaa ccgtgttagt ccccatctta gtaggtatcg
    28381 atccaggtat tgcgctagtc agttcaggac taggtacatt ggtgtactta tttactacta
    28441 aaggaaagat acctgcgtac ttaggaagca gcttcgcttt cattgcagcg atgcagcttt
    28501 tgatgaaaac agatggttat ccagcgattg cacaaggagc agtcacaact ggattagtct
    28561 atttgattgt tgcgctgatc atcaaaaaga tcggttcagc atggctggat aaaatcttgc
    28621 ctccaatcgt cgtcggtcca gtcatcatgg ttatcgggtt gggacttgcg gcaaatgcag
    28681 caacaaatgc tatgtataat cagggcacgt atgatttcaa gtatattgct gtagcgctga
    28741 tcacattagg attaacaatt ttcttcaaca tgtggctaag aggttttctc ggattgattc
    28801 cgattttact aggtattatt agcggataca tcgtagcctt gctgtttgga atcgtggata
    28861 ctaagccaat cgtagacgct gcctggtttg ctgttcctaa ctttgagatt ccctttgtac
    28921 aatatgaacc aaagctatat cttggagcaa tcacaacaat ggcacccatc gcttttgtga
    28981 cgatgacaga gcatatcggc catcttatgg tattgaataa actaactaaa cgtaatttct
    29041 ttgaagaacc gggtcttcat aagacgttga caggtgatgg attagcacaa atcgtggcag
    29101 cctttgtcgg aggtcctcct gtaacaagtt acggtgaaaa cattggtgtt ctggctatca
    29161 cacgtgtcca tagcgtgttt gtgatcggtg gagctgcagt atttgcagtc attttaggat
    29221 tcgtaggaaa attaagcgct ttgattttaa gtatccctgg accagtcatc tcaggaatca
    29281 gttttatctt atttggagta attgccgcaa gtggaatgaa gattttaatc gagaacaaaa
    29341 tcgattttga taaaaagaaa aatctgttga ttgcttcagt tatcttagta gtaggaattg
    29401 gcggactcgt ctttgaagta ggcacattta cattgtcggc aatggcatta gcaactgtac
    29461 ttggcatcat tttaaatctt atcttaccag aaacttcacg aagcgaagaa aactaaggag
    29521 gaaacattat gatcatcaaa tcagaacgta tcagcttaaa gcatttattg accgttgagg
    29581 cacttactga tcaagaagtt atgggcttga tccgcagagg gcaggaattc aagcaaggtg
    29641 ctaattggac tccgcaaaag cgccagtact ttgctacaaa cctctttttc gaaaatagta
    29701 ctcgcacaca taaaagcttc gatgtagcgg agaaaaaact agggctagaa gtgattgaat
    29761 ttgaagcaag cacaagctct gtccaaaaag gggaaacact atacgataca gtactgacga
    29821 tgtcagcatt aggcgtagac gtagcggtta tccgtcatgg tgatgagaac tattatgacg
    29881 aactgataca aagtaaaaca atccagtgtt cgattatcaa tggaggagac ggaagcggac
    29941 agcatccaac ccaatgcttg cttgatttga tgaccattta tgaagaattt ggtcattttg
    30001 aaggtctgaa agttgccatc gtaggtgata tcactcactc tcgcgtagct aaatccaata
    30061 tgcagatgtt aaaacgtcta ggcgcacaag tattcttctc aggtcctaga gaatggtacg
    30121 acgatgaatt cgaagtgtac ggacattata tgccattgga tgaactacta gatcaagttg
    30181 atgtcatgat gatgcttcgg gtgcaacacg aacgccatga tggaaaagaa agtttctcaa
    30241 aagaaggcta ccatcaggaa catgggttaa cagttgagcg tgcgaagaag ttgcaaaaac
    30301 atgcaatcat tatgcaccca gcaccagtta accgtgatgt agaattagct gattcattag
    30361 tggaaggatt acagtcgcgg atcgttcagc aaatgagcaa tggagtattc gtcagaatgg
    30421 caatcttaga agcagtgcta aatggaaaag cgtaattata ggaggatgaa aaaatgaaaa
    30481 cactcattaa aaacggtcag atcaatacac gaaaaaatat gacgacacca gcagaaattt
    30541 ggatagaaga tgggcgaatc aaagcaatcg gtacaggatt ctcagaagcc gaatttgatg
    30601 aagtgtttga tgcaaaggga caattgatca cacctggttt agtggacgtc catgttcatt
    30661 tgagagagcc aggatttaca tacaaggaga cgatcgaagc tgggactcgc tcagctgctc
    30721 gtggcggttt cactactgta tgcgcgatgc cgaatttaaa tcctgtacct gatacggcag
    30781 aaaagctgag acaagtatat gacatcatta gaaaagatgc agttgtgaag gtattgcagt
    30841 atgcgccaat cacagaaaat ctgcgaagtg agaaattagt tgatcaagaa gcactgattg
    30901 aagaaggtgc ttttgctttc acaaatgacg gtgtaggtgt ccaaacagct gggacgatgt
    30961 atctggctat gaaagaagct gcaaaaaaca ataaagccct agtagcccat acagaagatg
    31021 aatctttatt atttggcggc gttatgcacg ctggcaagaa ggcagaagaa cttggtcttc
    31081 ccgggatctt aagcgtaacc gagtcttctc aaattgctag agatctgttg ctagctgaag
    31141 caactggtgt tcattaccat gtttgccatg tttctactaa agaaagtgtc cgtgtaatca
    31201 gagacgcaaa aaaagcagga atccacgtaa cagcagaagt ttcaccccat catcttattt
    31261 tgatcgacga agatattccc gaagatttcg gcttttggaa aatgaacccg ccgctaagag
    31321 gacgggagga cagagaagct ttgatcgaag gcttgcttga cggtacgatc gattgcatcg
    31381 ctacagacca tgccccccat ggattagaag aaaaaagcca aagtttcatg aaatcaccat
    31441 ttggtatcgt aggaagcgaa acagcgtttc aactgatcta tactcatttt gtagaaactg
    31501 gtcgattcac gttggaacaa gtgatcaatt ggttggcagt taaaccggct gagatttttg
    31561 gtttgaatgc aggaacgcta acggttggtg cccccgctga tgtagcagtt tttgatatta
    31621 cacaaacttg cacgatcgat aaagaagact ttttatcaaa aggggagaat acacctttta
    31681 tcggctggaa agtaaaaggc gaaacacaaa tgacttttgt caacggaaaa cttgtgtggc
    31741 agaagggaga ataaaagtat ggaacgtttg cttatattag aagatggaac agttttcgaa
    31801 ggaaaggctt ttggtgcaga tgctagtgtc ttcggagaaa tcgtttttac aacaagtatg
    31861 actggttatc aagaaactat cactgatcaa agttttaacg gtcaaatcat tacatttacc
    31921 tatcctatgg tgggtaatta tggggtaaat agagatgatt atgaatcgat ttctccaaca
    31981 tgcaaaggag tagtagtaaa agaacatgca cgtgtagctt caaactggcg aaaccaaatg
    32041 acgctagatt catttttgaa acaaaaagga attcctggaa tatcaggtat cgatacgcgc
    32101 gcccttactc gcaaaatccg tgaagtcgga acgatgaaag ctagtatagt tgatgctgga
    32161 gattccttcg aacatgcatt tgatcagctg aaagcaaccg ttctagcaac caatcaagtg
    32221 cagcaagtct caactaacaa accttatcca agtccaggaa caggtcggaa agtcattgtc
    32281 attgacttcg gcttgaaaca tagtatttta cgagaattga gtaaaagaaa ctgtaacctc
    32341 acagtacttc cctataacac agatgccgag acgattttag cgctatcacc agacggagtc
    32401 atgctgacga atggacctgg tgatccaaaa gatgtgccag aagcaatcga aatgatcaaa
    32461 aaaatccaag gaaaagtacc gatttttggt atttgtctcg gtcaccagtt atttgcttta
    32521 gccaacggtg cagataccta taaaatgaaa ttcggacatc gaggattaaa tcacccggta
    32581 cgagaagtag cgaccggaag aattgatttt acttctcaaa atcatggcta tgcagtcgat
    32641 gaagccacga ttgatccaga aaaactcatc gtaactcatg tagaagtaaa tgacggaaca
    32701 gtagaaggag tcaaacacag agattatccc gctttcagtg tgcagtttca tccagatgct
    32761 gcccctggtc cgcatgatgc actacattta tttgatgaat ttatggaaat gatggacgca
    32821 ggaaaggagc agtaaacaga tgccgaaacg tacagatatc aagaaaatta tggtgattgg
    32881 atctggtccg atcatcattg gtcaagccgc agaatttgac tacgcaggaa cacaagcctg
    32941 ccttgcctta aaagaagaag gatatgaagt ggttttagta aattctaatc ctgcgacgat
    33001 catgacggat aaagaagtgg cagatcaagt atatatcgaa ccgatcacac ttgaatttgt
    33061 atcaaggatt cttcgaaaag agcgtccaga tgctctcctt ccaactttag gcggacagac
    33121 aggcctcaat atggcaatgg agctggcagc atcaggtatt ttagaagaat tgaacataga
    33181 acttctagga acaaagcttt ctgcgatcga tcaagcagaa gatcgtgatc tattcaaaca
    33241 gttgatgaaa gaattgaatc agccaatccc cgagtctgaa atcgtcaata cagtagaaga
    33301 agctgtagat tttgcaaata aaatcggcta tcctgtcatc gttcgtccag cctttacttt
    33361 aggcggaact ggtggaggta tgtgtgaaaa tgaagaagaa ttacgtatcg tatcagaaaa
    33421 tggattaagt ctttcccccg ctacccaatg tttgattgaa cgaagtattg caggttttaa
    33481 agaaattgaa tatgaagtga tgagagattc ggctgataat gcaatcgttg tctgcaatat
    33541 ggaaaatttt gacccggtgg gtatccatac aggtgattct atcgtatttg cccctagtca
    33601 aacattgtct gaccatgaat atcaaatgct tcgtgatgct tctctttcga ttattcgagc
    33661 acttaagatc gagggaggct gtaatgtcca actcgctctt gatccacata gttttaatta
    33721 ttatgtgatt gaagtcaatc cgcgtgtttc tcgttcatct gcgttagcaa gtaaggcgac
    33781 tggctatccg atcgctaagc tagctgctaa aatagcgctt ggattaacat tagacgaaat
    33841 gaaaaatcct gttacaggaa cgacttttgc agaatttgaa cctgcattag attatgttgt
    33901 agctaaaatt cctcgctggc cgtttgataa gtttgaaaac ggagaacggc ttttaggcac
    33961 gcagatgaaa gcaactggtg aagtgatggc tatcggtcgg aatatcgaag agtctttatt
    34021 gaaagctgtg cgttctttag aaatcggtac acaccatatc gaacttcctg agttggcaga
    34081 catcacggac gatgacctaa tccaaaaagt ggttcgagca caagatgatc gcctgtttta
    34141 tgtagctgaa gctattagaa gagggtacac catagaagaa ttagcggatt tgacaaaaat
    34201 cgatttattc tttttagata aactgttgca tatcgtagaa atcgaacagc agttgaaaca
    34261 gcagccgttg gatgacaaga tattgaaaac agcgaaacaa aacggtttta cagacaaaaa
    34321 agtggcggag ctgtgggaaa taaacgaaga agccgttcgc cagatgagaa aagaaaatga
    34381 tttgattcct gtatataaga tggttgacac ttgtgcagcg gaattcgaat caaaaacacc
    34441 ttatttttat agttcctatg aatatgaaaa tgaaagttca cgatcagaaa agccctcaat
    34501 tctagtactg ggatctggtc ctatccgtat cggacaaggg gttgaatttg actatgcgac
    34561 tgtgcattct gtcaaagcaa tccagcaggc aggatacgaa gcaatcatca tgaacagcaa
    34621 tcctgaaaca gtttcgactg acttttctat ttcggataaa ttatattttg agccattgac
    34681 cttcgaagat gtgatgaatg tgatcgagct agaacagccg ataggagtaa tcgttcaatt
    34741 tggtggtcag acagcaatca acttagccga acctttgact gctgctggtg taaaaatctt
    34801 agggacaacg atcgaagact tggatcgtgc ggaaaatcgt gatttattcg agcaagcgct
    34861 gaaagagttg gatattcctc aacctttagg ggatactgct acaagtaaag aagaagccgt
    34921 ggttatcgct tcgagaattg gttatccagt tcttgttcgt ccaagctatg tattaggcgg
    34981 acgtgcgatg gaaatcgttg aaaaccagcg agatttagaa gattacatgg aacatgctgt
    35041 gaaagcatct ccagaacatc cagtactagt ggaccgttac ttgcttggtg gtgaatgcga
    35101 agtggatgcg atttgtgacg gtgaaacagt actcatccca gggatcatgg agcatattga
    35161 gcgtgctggt gtccattcag gggattcaat ggctgtgtat ccacctcaaa cattatctga
    35221 atcgatcaag aaaacgatcg aagattacac gatccgctta gcattaggtt tgaattgtgt
    35281 cgggatgatg aatatccagt ttgtcatcca tgaaaatgag gtttatgtga tcgaagtcaa
    35341 tcctcgagca agtcggactg ttccattctt aagtaaggtg acaggaattc caatggcaca
    35401 aattgcgacg aaagctattt tgggagagaa actaaagaca cttggttatg aaaacggact
    35461 ttatccagaa acaaaagaag ttcatgttaa agcacctgtc ttttctttta cgaaattgca
    35521 gaaagttgat acttacttag ggcctgaaat gaaatcgact ggagaagtaa tgggatctga
    35581 cagacaatta gacaaagctt tgtacaaggc attcgaagct tctggattac acttgcctga
    35641 atatggagcc gtattattta cgattgcgga cgaaacaaaa gaagaagcac tagaattagc
    35701 taaacgtttt acagaaattg gctatagtct gataggaacg aaacatacag ctgcttattt
    35761 cgaaaaaaat ggtctggtcg ttgaaacagt agcgaaaatt tctgaggaag cagcagaaaa
    35821 aaatgtagtc gacttaataa gagatggtaa aacacaagtg gttgtcaata cgattgataa
    35881 agatcgggga aatgcttcta aagacggttt catcatccga agagaagcag ttgaacatgg
    35941 tattccattg tttacttctt tagacacagc agatgcgatc gttcgagtga tggaatcccg
    36001 tgctttttca actaacccta tttaacggcg agcacatgtt aagacaaaaa gacgaaaaac
    36061 cgttgcagga ggggacagat gaaacaagaa atgatggaaa tcgttcagca aaaacagctg
    36121 gcaccaaaga tttttgaaat gacattgaaa ggtgatctag taaaagaaat gcagcaacca
    36181 ggacagttct tgcatattct cgtgccgcgt gcagacttgc tattacgccg accgattagc
    36241 ttgaatcata tcgaccacga aacaaatact tgccgtatca tttatcgggt ggaaggtgag
    36301 gggactagag tcttctctac tctttctgcc ggagaccatt tagatgtcat gggtccttta
    36361 ggcaatggtt ttgatttgtc caatcttcat acaggagacg aagtatttat aatcggtgga
    36421 ggaatcggtg tccctcctct atatgagtta tcaaaacagt tagtcgaaaa aggaattaag
    36481 ccaacgcatt ttttaggttt tgccacacat gaagtcgttt attatgaaaa agaatttaat
    36541 cgtctaggag aaacaagaat cgctactgac gatggaacat acggtatgca cggaaatgtc
    36601 ggtaatttat tgttaggaac gaagacggaa cctacagctg tttttgcatg tggttcaaac
    36661 gggttattga agacagtgga acagctgttt tcttcgcatc aaaatgtcca gttatcatta
    36721 gaatcccgca tggcatgtgg gataggggct tgctatgctt gcgtttgtca tacgccggaa
    36781 gatgaaacag gaacaaaaag tgtgaaagtt tgtgacgaag gaccgatttt taaaatagga
    36841 gaggtaatta tatgaatgag aatcctttag cggtcaaact tcctggccta gaattaaaaa
    36901 atcctatcat gccggcaagc ggctgtttcg gttttggcaa agaatacgga aaatactatg
    36961 atttgaatca gcttggatcg atcatggtca aggcaacgac tccaaatgcc cgctttggca
    37021 atcccacgcc aagagtggcg gaaacaccat ctggcatgtt gaatgcaatt ggtttgcaaa
    37081 atcctggtct agacgttgtt atgcataaac ttcttccaga attagaggag tacaatgagt
    37141 tacccatcat cgcaaatgtt gctggtgctt gtgaagaaga ctatgtagaa gtttgtagca
    37201 aaattggcga agcaccaaat gtgaaagcaa tcgagctgaa catttcttgc ccaaatgtca
    37261 aacatggcgg aattgctttt ggaacagatt ctgatgttgc ttttcaatta acacaagcag
    37321 taaagaaagt ttcttctgtt cctgtctatg tcaaattgtc cccaaatgta acagacatcg
    37381 ttccaatcgc tcaggcaatc gaagctggag gggcagatgg gtttacgatg atcaatactc
    37441 ttcttggtat gcggattgac cttaagacaa gaaaaccgat tttagccaat caaacaggtg
    37501 gactctccgg accagcaatc aagcctgtcg ctattcgttt gattcaccaa gtagcaagta
    37561 tatccagcct tccaatcatt gggatgggcg gcgtgcagac agtggatgat gttcttgaga
    37621 tgtttatggc tggagcaagt gctgtagcta tagggaccgc gaatttcacc gatccgtata
    37681 tctgtcctaa actaattaaa gagctccctg tgcgaatgag cgaactaggc atcgaatcgc
    37741 tggaaagatt aatcaaagaa gtgagagagg aacgtgaacg atgaatcaac tagaccaacg
    37801 accaattatt gcattagatt tttcaactag gcaagaggta gaagactttc ttcgattttt
    37861 cccgaaagaa gaaaaattgt ttgtgaaaat cggaatggaa ttgttctacc aagaaggacc
    37921 tgaaatcgta cgttatctaa aagaggctgg tcataatgta tttttagatc tgaaacttca
    37981 cgatattccg aatacggtcg aaaaagcaat gcggggattg gcaaagcttg gtgttgatct
    38041 cacctgtgtt catgccgctg gcggtattcg tatgatggag gcggcaatgc gaggattaga
    38101 agaggggact ccagaaggtg gaaaacgtcc gctacttcta gctatcactc agcttacttc
    38161 tacaagtgaa gaagaaatgc atgccgatca actaatcgaa gtttctttag aaaaaagtgt
    38221 gatccattat gctagctgtg ccaaaaaagc aggattagat ggtgtggttt cttctgcttg
    38281 ggaagtagaa gcgatcaaag aaatggcagg agacgaattt gtttgcttaa ctccgggtat
    38341 aagaccagaa ggaacagtcg caggagatca gacaagagta gtcacacctt ctcaggcaaa
    38401 aaagatcggc tcaactttta tcgttatagg tcgaccaatc actcagtcaa ctaatcctta
    38461 taaagcatat caaacgattc aaacagaatg gagctaacct aaaatgaatg ttgaacaaag
    38521 tatcgcaaaa gacttattag aaattgaagc agtattttta aatccaagta atccttttac
    38581 atgggcaagt ggaatcaaaa gcccaattta ctgtgataac cgaatcacga tgagctatcc
    38641 aaaagtccga aaagagatcg caaaaggatt agcaagcaaa atcaaagaag cttaccctga
    38701 agtacaagtg attgctggaa ctgcaacagc aggaatccct catgccgcat gggtagcgga
    38761 aatactggat ttgccaatgg tctatattcg aagtaaggcg aaagatcatg ggaaaggtaa
    38821 ccaaatcgaa ggacgtatat ttgaaggtca gaagatggtt gtgatcgagg atttgatttc
    38881 tacaggcgga agtgtcttgg aagcagctga agcagcaaaa agagaaggtg cggatatcct
    38941 tggtgttgcg gctatcttta cgtatgagtt gccaaaagga aaagcaaatt tcgaaaaagc
    39001 cgaaatccct cttatgacgt tgaccaatta ttctgtattg attgaagcag ctttagaaga
    39061 tcgttatatc gatgagcaag aattaactct gctaaaagaa tggaaaaaag atcctgaaaa
    39121 ttggcagatt gaagcgcgct tgatgaagat agagaaatcc gatttgtgtc tccagaatca
    39181 tcaattggaa tagcaaaaat ccaaaaactg attgcgagaa gcatcttttc atcaggtatg
    39241 aaaagaaact tctatcgtga tcagtttttg atttttttaa aattcatttt tctaagactg
    39301 gttagcagaa agaagagaat aatctcttct cttttttcta ttatatatag attttctcta
    39361 ttttttggta agggagctgt ttcaaaaatg gtaaagagcg gaaatgttgc gaatatcagt
    39421 tatttaatga tatgatattc ggaaaaagaa atctcaaagg gaggtaactt ttatggggtt
    39481 gaatgaattt attttaggat tgaacaattt agagacgatc acaagggcac ctggattttt
    39541 taaatataca gagcatacag ttgcagcaca ttcgtatcgt gtagcatcta ttgcgcaagt
    39601 tctaggagac attgaagaag cgaatggaca accgatcgat tggaaactgc tctatgaaaa
    39661 atcattgaat catgactaca cagagcgttt tattggtgat atcaaaacgc cggtgaaata
    39721 cgcaagcaaa gagttaagag ggatgctgca aacagttgaa gaaaagatga cggacgaatt
    39781 catcaaccaa gagattccag ctgagttcca agaagtttat cgccgtcggt tgttcgaagg
    39841 aaaagatgaa accgtagaag gagaactatt agccatttca gataaggttg accttcttta
    39901 tgaatctttt gatgaaatca tcaaaaataa cccggaaaaa gtttataagg agatgttcat
    39961 tgaggcagtg actacgatta aaagctttga acatcggcct tctgttcagt atttctttaa
    40021 agagatattt ccggaactat tgaaccagga attttatgga aaagaggcgt ttttagaaac
    40081 cgtctcaacg attgtaaaga aagaagaaaa ataaaaatga tatgagatgc agggggaagt
    40141 atagttgaaa aagaatatgg atgtcgaatg gggctattct ggagacaaag gtccggaaaa
    40201 ttgggcgagt ttatgtgatt ggtttgcgag aggtgcggaa tttccattgc agtcgcctat
    40261 tcatttgacc aagggggaag aaaccaaaat agaaggagac acgctttcct ttcattatca
    40321 aaagcaacgg tttactgaca aggaattcaa aaacacgatt catttagtac cttttgacca
    40381 actcagttat gtagagttca agaaagaacg ctattatttg acagatattc atttccactt
    40441 gccttctgaa catatcatca acggaaaaca ggaagatatt gaatttcatt ttgttcatat
    40501 gaacagcaaa aaagagaatc tagttgtggg tgtcatgtac cagctaatgg tacaggaagg
    40561 ttggctgtat aatcaagaaa atggggaaag ttggaactta gaaaaacatg agcattgggt
    40621 aaatccggat gtgttctttc cggagcaaaa aagccatttc cattatattg gctcattgac
    40681 aactccgcct accagcgggc caatccaatg gtttgtaatg gatcatgtag gaaaactgaa
    40741 ccaagagttt cttctcccct tcgatggtca gtatgctcga ccgaacaacc gaccaatcca
    40801 gcccttgaat ggaagagaaa tttattattt cgaggagttt gagcaatgaa tattcagcag
    40861 atgaaatacg tggtcgctgt agcaaacaat ggtagtttca gagaagcggc aaagaaaatg
    40921 tttgtctccc agccaagtct ttcagctggt atcaaagaat tagaaaccga gttagggatc
    40981 acgctgttta ctcgaacgaa tcgaggggca tatttaacag aagaagggca agacttctta
    41041 aatcgtgccg agaaaatatt ggtccagctt gatacattag aaactcacta tctaacgagt
    41101 gaaaagagag acaatttttc gatcgctgcc cagcattatg atttcttagg tcctttgacc
    41161 gcacagctac tcgaggaatt caaagacgag atcaaacaat ttcggatcat ggagacgaca
    41221 acagcgagag tgatcgaaga agtcaaagag cagcacagtg agatcggcat tatttatatg
    41281 aatgagcata atcaagctgg tatcagaaga tatttacaac aaggagagct aagtggccgc
    41341 agcattggaa aattcaaaac gcatattttt ttaaggcaag gacatccatt agctgaacac
    41401 gcagagatca ccaaagaaga attgttatct tatcctcaag ttcgttttac acaggatgga
    41461 aataatttcc cgtattttta tgaagacctt atcgagatcc ctgatcaaga aacagtgatt
    41521 tatacgagtg atcgaggtac tttgatgaat atcgttcttg aaactgatgc ttacgcttca
    41581 ggatctggta ttgtgattgg agggattcgc gagcacttgc ggttaatccc tttggcagaa
    41641 ggcgaactaa atgaatttta tatcatttat tcggcgaaac gtccgctcag tgaaatcgct
    41701 caaagattta tcaaagagct tactcggtta ctggaccagc agagctaaga aaagcagatg
    41761 agaacgctgt ttattgaaca ccgttgatca aaaaagaggg gttgtgacag aattttgtca
    41821 caactcctct ttttgtagca aacaatttat aactaattgc tgttgtggga aagtacgcaa
    41881 atatgattct agaaacactc attgattacc tgttttgttt catagctgta attttttctt
    41941 cttctggtgt cacgataatc gtcttttggt tttcataaat aacataacca ggcttggcgc
    42001 catttggttt tcggatatgc tttacctgta caagatcaac cggcacttgg gcagagtatc
    42061 ggtattttga aaaataggca gctaactcgg cagcttctgt aattgtttca tctgagggct
    42121 tgtcactttt aatgatgacg tgcgaaccgg gtatattttt tgcatgaagc caatagtcgg
    42181 tttttttagc tgtcttcatt gtcagctggt cattttgaag attgttttta ccaacaagaa
    42241 tgagcgttcc gtcagaagaa aagtattgat caggctgaga tggtttctta cgtttttgtt
    42301 tttttaagtt cttctttttc aggtatcctt ctgaagtcaa ttcttcttta attgcttcga
    42361 tatccattgg gccggcaatt tccagttgag ataagacaga ttcaagatat tggatttcat
    42421 ctttggcttc ttggatttgt tcgccaatca gcttaacagc gttcttcagc ttttggtatc
    42481 gatggaaata tttctgtgca ttttgatttg gtgtcaatgc cggatctagt gctattttga
    42541 ttggccgatc ttcttcataa taattaggta aaacaacttc ttttgcgccg cgtggtactt
    42601 gtgtcagaaa agtagtaagt agttcgccat ttctacggta attctcagcg ttttcggatt
    42661 ctttgagtgt ttgttcacgt tttttcaatt tatttttatt tcgttttagt tcattttcga
    42721 tttttcgaat cagttctccg ccttgttgtt ttgcccgatc tttctctgct ttttcgtgat
    42781 aatagctatc taataattga ctgagtgttg gataagctgt tacagcagat gcctgttcgg
    42841 acaatacctg ataagcaatc ggtgtgaaaa aatctttatt ttccgtttcg taaaaagtag
    42901 gaataggctg atggctgatg gctgagaaaa attcgtccca tacaaccatc ttttcattag
    42961 gacgttccag taatcttttg gtcagctctt gagcgatatc gaaaccgatt ccctgaaact
    43021 gacgttggat tgcttttgga tctagttcag tttgggaaag ccgctgaaag attttttctt
    43081 tctcggagct aaatgggttc aagacctctt gttttggcgg agcaacatat tctacaccag
    43141 gaagcaaaga acgataagtg ttttgagagc ttccaatgtg tttgatcgca tccagtattt
    43201 ttccagtttc tcgattaact aagacgatcg tactatgacg tcccattaat tcaacgatca
    43261 atatgatatt ttgtagatca cccaactcgt ttcgtttagc aaagtggaaa tggatcactc
    43321 gatcattttc gatttgttcg atgtcttcta aaatagcgcc atccaaatat tttcgaagca
    43381 tcatcacgaa gttaggcgga ttatcaggat tctgataatc gatttgggta atctgtacac
    43441 gagcataact tgggtgcgca gataataata atcgatggtt tttgccgcgt gaacgaatca
    43501 ccagcacgat ttcattttca taaggctgat ggattttcga gatccggcca gatagtaacg
    43561 tttcacgcaa ttcgttgacc atcgcgtggg taaatacacc atcaaaggac atggttcatt
    43621 cctcatttct tttcataagt atattcatta taacgaaaaa aagaagaaaa accaatttgg
    43681 ccgaatagta aagcaaaagt ctagaaaaat cttggactaa ttacataaaa aacagtgttt
    43741 ctatcgttga aatccaaatt tttaggt
//
LOCUS       DACDRK010000015        43718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDRK010000015 DACDRK010000000
VERSION     DACDRK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.015"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            158..1798
                     /locus_tag="IWJ77_000916"
     CDS             158..1798
                     /locus_tag="IWJ77_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9452554.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1952..3274
                     /locus_tag="IWJ77_000917"
     CDS             1952..3274
                     /locus_tag="IWJ77_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9452555.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3342..3776)
                     /locus_tag="IWJ77_000918"
     CDS             complement(3342..3776)
                     /locus_tag="IWJ77_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9452556.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(3841..4678)
                     /locus_tag="IWJ77_000919"
                     /pseudo
     CDS             complement(3841..4678)
                     /locus_tag="IWJ77_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(5420..6046)
                     /locus_tag="IWJ77_000920"
     CDS             complement(5420..6046)
                     /locus_tag="IWJ77_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452557.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(6341..6676)
                     /locus_tag="IWJ77_000921"
     CDS             complement(6341..6676)
                     /locus_tag="IWJ77_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9452558.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(6663..6947)
                     /locus_tag="IWJ77_000922"
     CDS             complement(6663..6947)
                     /locus_tag="IWJ77_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9452559.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7003..8526)
                     /locus_tag="IWJ77_000923"
     CDS             complement(7003..8526)
                     /locus_tag="IWJ77_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9452560.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(8519..9694)
                     /locus_tag="IWJ77_000924"
     CDS             complement(8519..9694)
                     /locus_tag="IWJ77_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9452561.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(9698..9883)
                     /locus_tag="IWJ77_000925"
     CDS             complement(9698..9883)
                     /locus_tag="IWJ77_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452562.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(9849..11543)
                     /locus_tag="IWJ77_000926"
     CDS             complement(9849..11543)
                     /locus_tag="IWJ77_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9452563.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(11540..12013)
                     /locus_tag="IWJ77_000927"
     CDS             complement(11540..12013)
                     /locus_tag="IWJ77_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9452564.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12082..12237)
                     /locus_tag="IWJ77_000928"
     CDS             complement(12082..12237)
                     /locus_tag="IWJ77_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9452565.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(12371..12751)
                     /locus_tag="IWJ77_000929"
     CDS             complement(12371..12751)
                     /locus_tag="IWJ77_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9452566.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(12755..12970)
                     /locus_tag="IWJ77_000930"
     CDS             complement(12755..12970)
                     /locus_tag="IWJ77_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452567.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(12973..13380)
                     /locus_tag="IWJ77_000931"
     CDS             complement(12973..13380)
                     /locus_tag="IWJ77_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9452568.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(13646..15121)
                     /locus_tag="IWJ77_000932"
     CDS             complement(13646..15121)
                     /locus_tag="IWJ77_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9452569.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15111..15959)
                     /locus_tag="IWJ77_000933"
     CDS             complement(15111..15959)
                     /locus_tag="IWJ77_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9452570.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(15996..16358)
                     /locus_tag="IWJ77_000934"
     CDS             complement(15996..16358)
                     /locus_tag="IWJ77_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452571.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(16658..17282)
                     /locus_tag="IWJ77_000935"
                     /pseudo
     CDS             complement(16658..17282)
                     /locus_tag="IWJ77_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(17279..17590)
                     /locus_tag="IWJ77_000936"
     CDS             complement(17279..17590)
                     /locus_tag="IWJ77_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452572.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(17634..17927)
                     /locus_tag="IWJ77_000937"
     CDS             complement(17634..17927)
                     /locus_tag="IWJ77_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452573.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18121..18768
                     /locus_tag="IWJ77_000938"
     CDS             18121..18768
                     /locus_tag="IWJ77_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9452574.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            18829..19974
                     /locus_tag="IWJ77_000939"
     CDS             18829..19974
                     /locus_tag="IWJ77_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9452575.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(20055..20360)
                     /locus_tag="IWJ77_000940"
     CDS             complement(20055..20360)
                     /locus_tag="IWJ77_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9452576.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(20385..20816)
                     /gene="ruvX"
                     /locus_tag="IWJ77_000941"
     CDS             complement(20385..20816)
                     /gene="ruvX"
                     /locus_tag="IWJ77_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9452577.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(20813..21079)
                     /locus_tag="IWJ77_000942"
     CDS             complement(20813..21079)
                     /locus_tag="IWJ77_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9452578.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(21334..22059)
                     /locus_tag="IWJ77_000943"
     CDS             complement(21334..22059)
                     /locus_tag="IWJ77_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9452579.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(22420..22490)
                     /locus_tag="IWJ77_000944"
     tRNA            complement(22420..22490)
                     /locus_tag="IWJ77_000944"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22456..22458),aa:Gly,seq:ccc)
     gene            22607..23359
                     /locus_tag="IWJ77_000945"
     CDS             22607..23359
                     /locus_tag="IWJ77_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9452580.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            23375..23854
                     /locus_tag="IWJ77_000946"
     CDS             23375..23854
                     /locus_tag="IWJ77_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9452581.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(24105..25685)
                     /locus_tag="IWJ77_000947"
     CDS             complement(24105..25685)
                     /locus_tag="IWJ77_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9452582.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(25678..26421)
                     /locus_tag="IWJ77_000948"
     CDS             complement(25678..26421)
                     /locus_tag="IWJ77_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452583.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(26597..27373)
                     /locus_tag="IWJ77_000949"
     CDS             complement(26597..27373)
                     /locus_tag="IWJ77_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9452584.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(27621..28457)
                     /locus_tag="IWJ77_000950"
     CDS             complement(27621..28457)
                     /locus_tag="IWJ77_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9452585.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(28468..29397)
                     /locus_tag="IWJ77_000951"
     CDS             complement(28468..29397)
                     /locus_tag="IWJ77_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9452586.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            29589..29942
                     /locus_tag="IWJ77_000952"
     CDS             29589..29942
                     /locus_tag="IWJ77_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9452587.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(30016..30672)
                     /locus_tag="IWJ77_000953"
     CDS             complement(30016..30672)
                     /locus_tag="IWJ77_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9452588.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            30898..32832
                     /locus_tag="IWJ77_000954"
     CDS             30898..32832
                     /locus_tag="IWJ77_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9452589.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(32918..34141)
                     /locus_tag="IWJ77_000955"
     CDS             complement(32918..34141)
                     /locus_tag="IWJ77_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9452590.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(34135..36033)
                     /locus_tag="IWJ77_000956"
     CDS             complement(34135..36033)
                     /locus_tag="IWJ77_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9452591.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(36027..37145)
                     /locus_tag="IWJ77_000957"
     CDS             complement(36027..37145)
                     /locus_tag="IWJ77_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9452592.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(37228..38061)
                     /locus_tag="IWJ77_000958"
     CDS             complement(37228..38061)
                     /locus_tag="IWJ77_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9452593.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(38054..38971)
                     /locus_tag="IWJ77_000959"
     CDS             complement(38054..38971)
                     /locus_tag="IWJ77_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9452594.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(39198..40322)
                     /locus_tag="IWJ77_000960"
     CDS             complement(39198..40322)
                     /locus_tag="IWJ77_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9452595.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            40505..41413
                     /locus_tag="IWJ77_000961"
     CDS             40505..41413
                     /locus_tag="IWJ77_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9452596.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            41554..43167
                     /locus_tag="IWJ77_000962"
     CDS             41554..43167
                     /locus_tag="IWJ77_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9452597.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        13253 a         8691 c         7310 g        14464 t
ORIGIN      
        1 aagcaaggat tgtcgatata aaaagaacaa atactgctat tttgatttct tttggctata
       61 ttacatataa ataaaattta tcatacctgc tctttttgac tatatcatca tctgacttct
      121 atacttcgtt tgtattaaaa cttaggagga atcattcatg cttacagggt taaacttatt
      181 aaataatcct tttctaaata aaggaacagc ttttacaaaa gaagaacggg caaaatatgg
      241 aatcactggt atgcttccaa gtactgtcca aacactggaa caacaatctg ttcaagctta
      301 tggtcaatac ttaagcaaac aaacagattt ggaaaaacgt atcttcttaa tgaatctatt
      361 caatacgaat cggacattgt tttataaatt gatgggcgaa catttagttg aattcatgcc
      421 tgtcgtttat gatcctgtcg ttgcggattc aatcgaacaa tacaacgaaa ttttcttgaa
      481 accgcaagat gctgcatttc tttcaatcga cgaaccagaa aacatcaaag caagtttgaa
      541 gaacgcggca gatggacgag acattcgttt gattgttgtg actgatgcag aaggaatctt
      601 aggtatgggc gactggggtg tgaacggtgt tgacatcgct atcggcaaat tgatggtcta
      661 tactgctgct gctggtatta atccagcaca agttttgcct gtatcgatcg acgctggtac
      721 taataatgaa gaattattga ataatccctt atacttaggc aatcgtcatg ctcgtgtaga
      781 aggcgaaact tactatgaat tcatcgatca attcgttcaa tcagctaccg aattattccc
      841 agaattgtta ttgcattggg aagattttgg acgaggaaat gctgcagcga tcttagaaaa
      901 atacgaagat caaatcacta ctttcaacga cgatatccaa ggtacaggaa ttgttgtcct
      961 agcaggtgta ttaggcggat tgaatatatc cggtgaacaa ttgaaagatc aaataatttt
     1021 gacatttggt gctggtactg ctggtgtggg tatcgcaaac atccttttag atgagatgat
     1081 ccgtcaaggt gtacctgaag aagaagcacg ccaacatttc tatcaagtag ataaacaagg
     1141 tttattgttt gaagatacag aaggattgac tcctggtcaa atcccattcg ctcgcaaacg
     1201 ctcagatttc actaatgctg atgaattgac aaacttagaa gcagttgtaa aagcgatcca
     1261 tccgacaatc atgattggta cttctaccca acctggagca ttcactgaag aaatcgtgaa
     1321 agaaatggct gctcatacac cacgtccgat catcatgcct ttgtctaatc caacaaaatt
     1381 ggcagaagca aaagcaaaag atttaataga atggacggac ggaaaagcat tagttggaac
     1441 aggtatccct gctgctgatg ttgaatataa tggtgtaact taccaaatcg gtcaagcaaa
     1501 taatgctttg atgtacccag gtttaggact tggtttgatt gcttcaacag ctacacgagt
     1561 aaatgctgaa atcatttctc aagctagtcg agcattaggc ggaatcgttg atgtaacaaa
     1621 accaggagca gctatcttgc ctccagtagc aaaaatcact gaattttctc aaacgattgc
     1681 tgaaactgtc gcaaaatcag ttgtcgcaca aaacttgaat cgtgaagaaa tcacggatat
     1741 caaagaagca gttgaatctg ctaaatgggt tcctgaatat aaatctttag aagattaaga
     1801 tcgttatagt aaaagaacat acagaaactt tgtatgacgt ctaaaaagaa aaaagcgact
     1861 gcggcaaaac agcggccagc caactcctgc tcttttgtcg cttttctttg gcttttttca
     1921 tatgatacag aataagaaag gatctgtaaa aatggcacaa aaaacaacaa ctgcaactgc
     1981 agttgaggaa aagaaaaaag aaggtttttg gtccacaaaa atcaccggcg taagtttgcc
     2041 actttatttg atcatggtcc ttgtcctgat cattgcaatg gctttaggga aattaccgac
     2101 gaatatgatt ggaccaatcg cagttttggt tattttcggt aatttatttc attttatcgg
     2161 gacaaaaatc cctatcgtca aaagttattt gggtggtggt tccgtttttg cgatcttcgc
     2221 ttctgctgcg attgctacat ttgggatctt acctgaatat gtagtgaaat ccacagaaaa
     2281 ctttgtcagc aatatgggct tttgggactt ttatattgct gccttgatta ctggtagcat
     2341 ccttggtatg aaccgtaatc tattgatgaa agcttctgtt cgttttattc ccgttgcact
     2401 tatttcaatg gtcgtctcct tttttgccgt aggactcgtg ggcatgctta tcggaaatgg
     2461 ttttgcaaat tccgttttat acatctcatt acctgcgatg gctggtggtg ttggagccgg
     2521 agccgttcct ttatcaacga tttatgcaaa tgttttaggt acggatgctt cttctatcat
     2581 ttcccgcctg attcctgcga cagcaatgac aaatgtctta gctatcatcg gtgcagcatt
     2641 agttgctcgt gctggacaat caatgccaaa attcaacggc aatggtaaat taatggaaaa
     2701 aggcgatctt ggcgatggaa aacctcgaac tgtgaaacct tccattccac agttaggtgt
     2761 tggtttgatg gtatcattga ctttcttcat cttaggacaa attggtaact attttgttcc
     2821 taaagtccat gcttacgcat ttatgattat tatcgttgtc atctgtaaaa tcgccaacct
     2881 attaccagag tattatgaag atgcagctat catgttcaat aacttgattg tcaaaaactt
     2941 gacagcagct gttcttgccg gtatcggtat tgcgttgctg aatctgaatg tattagcttc
     3001 tgctttgaca tggcaatttg ttgtcttatg tctgacaagt gttatcgcga tttcagttgt
     3061 ttcaggattt gtcggtcgtt tgtttggttt atatccaatt gaatcaacta ttactgctgg
     3121 cttatgcaac aactcgatgg gcggaacagg aaatgtagcc gtcttatctg cagctaatcg
     3181 gatggaactt attgcttttg ctcaaatggg caatcgactt ggtggtgcca tcgttttgat
     3241 catctctggt ttcttgatgc aattactttc ttagtcaaaa aaacaaaaat acctgatcat
     3301 caagtatcgg cttggtgatc gggtattttt ttgataaaga tttacaggat tttcaaaaat
     3361 cttttttgca ttttatcgga atgataaccg atgtgttcat aaaattggtg tgcttctact
     3421 ctgtattctg cagaattaag ccttattgtc gttattcctc gttttttcgc ttcttcttct
     3481 acgccagtca tcaaagcctt tccgatgcct cttttttcag catatttaga cacagcaagt
     3541 gccataatat tgaacattgg atcgctaaaa atcgattcat aggactccgc ttgtacatag
     3601 ccagctgctt ttttagaagt aggatcttca taaaccaaga aaaaattttt ctctggctcc
     3661 ttcagcagtt tcttcatttg tctcaccgta acggttaatg gtatatcata ccctaattga
     3721 tccgcattga tttcacgtag tgcttctgca tcttctaaac gcatttcccg taacatccaa
     3781 atcaccagcc aatttctgat tttttaaaat gaattttttt atcatagtat actctttttt
     3841 tcatttagaa caatatgttt cagtgatttt gacttgcgtt agtctagatt ctcctaatgt
     3901 ttgtaagatg acaaaggcat tgtaacaatt gaccaaaaga accacgagga caattacttg
     3961 aaaaataaag gtcacttttt ttggagacaa aaccctgcta aatcttgctc cccacaaacc
     4021 tccaataaca gcagcgggga taacataaaa caacatagat aaatcaaagc gcataaagct
     4081 acttgttaaa gcaatcgtta ccaattttgc cagttgagaa aagaaaatcg tagatagaga
     4141 atatagtgtt gcttctttta ttggcaatgc aaacatcagc atcaacaaag aaacattgac
     4201 tggtccacca ccaataccta aaaaactagc taaaaagccc agaacccccc ccacatataa
     4261 ggtaccaagt ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg tagaagaacg
     4321 caaaaaccaa ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc ttctcgtttt
     4381 caaataactg taaaaaaact tcgaatacaa tgttgccgac tactcctcca acaacagctc
     4441 cactagatac ccaaccgaca atctgccaat tcagtgattt cccgcttgat acttgtcgca
     4501 cagtcgaaac aatggacatc gtgaataccg caactgttga gtaaaaagaa atccctgcaa
     4561 cagaatgggc gccgataaga tcaagaatcg gtttgatcaa cacgcctcca cccatcccgg
     4621 aaatagcgcc aactgtgttc gccagaacga tcaccacaaa ataaatcaga ccaatcatct
     4681 tgtatttcct ttctcttgtt tatatctttt agcgattgtt actcttttct tttattatac
     4741 agtaaaagaa acaaacgagg cttcgaaagc ttttctactt tttaaggaaa tttagtgcta
     4801 aacgaaaaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa
     4861 atagctcctc ataattttcg tttggttgaa cttattaccg cagatgcaag cttttgaaca
     4921 ccgtttaaga aagaggtcgt gagcctgccg ttcagctccg agtcacaagg aacaatttca
     4981 aaaaacagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt tggcaggaga
     5041 acaccgttta aggaagagac tgtgaagtca acgttttgac ctgattatta gagaaacaaa
     5101 tggcaaaata gttcttcata tttcgccgtt tttcgagcga atgccgaggg gcagctgatt
     5161 gaacatcgtt tatttggaaa aagggattgt gacaagacgt ttgtcacaat cccttttttt
     5221 atatcttatt aattcattct tttgttctat tcttcttctt ccgccatgaa tgagaaaccc
     5281 atatcaaaga actataaagg ttatcaaaac agcgtttatg actaaatgaa ttagagttat
     5341 ctatcactat ttttcaagcg atttaaacca aatggcatac aatatggcat acaaaaactt
     5401 caataagccg tgggggtttt taaatttctt cttgttcttt aaaatcaatg agtggagcta
     5461 cataatttga atccactttt tcaaacgtca ctttttgcat cgacgaatgt tttctttccc
     5521 aagataacgt aaatagattt gactctggac ttataaagtc tacttcacct tctagtatta
     5581 ctttcttatg caaatattcc tctaataaat atagacttgc taaagcatac aacacatttt
     5641 ccaaatttgc ttccttataa tttgaagaac gaccatgttt tactctgtta tatattttcc
     5701 accaatctgg tgtatcttca ggccacccat ctccaaaagg ttttagtgaa gtcaatgtta
     5761 aagaattcat aaccctaaca ccattatcca aacacattaa ttcgctagat ttaataacag
     5821 ctttaaaatc agttatgctt ttattgctac tgctatcaaa tccgcaaagt tctttgaaaa
     5881 taatctcaca ctccgaacca atcgctagta gtagtttttg atattcaata gagaatgttt
     5941 tgaaattatt ctcatcgagc atcacaaaat ctgtggtatt aataaactca ttttctaaat
     6001 gtagataata cttccaataa aaatttgtaa actctgcttt gtccattaaa tcacctcttt
     6061 tgaattataa caaaaaaaac acgtccacaa cagacgtgtt ctttgatttc tacacaatca
     6121 gcttgccaaa accatccaat cagtcctagc atgcctgtat taaaattgaa cgttcatcat
     6181 ctatcatctt tcttagtcca gcatgtttgc aaccacacct acaattttca gtccaacaat
     6241 agatttcgac tagctaagtc ttgcaaaatt caggcagcta acccttcatt tactacctta
     6301 attatactaa atacccacaa cgtttacaag tgtaatcatt ctaagaaatc tgtttagcta
     6361 tgatcgttgt ataatcttta aacgccgttc cttcattgat tgaaataatt ttataactct
     6421 tgtttttcca agctactttc atatcgtttg ttagttcata atcttgatcg tatcgaataa
     6481 taaacgtcaa cgttccttca agaatggtac ctacactagc aagcacgtct tttagcattt
     6541 gtgaacgcac attagcccaa cattcaaata cagtttcttc actatccacc atctgcccat
     6601 cttcatcttt tacccgtttt gtctttttaa acttgactct ctcagtcaat tcatttacat
     6661 tcttaatgat tggcataata agcacctctc atttgttgta ctaatgcttg taccgttact
     6721 ggtatccgtt cgctgctggc ttcttgacgg ttcaaatacc aatgttgagt aagtaatgac
     6781 accgccaaat caaattgctg ataattatcc atatctttat ccgttgcgct actatcaata
     6841 gcacttttta tatattcgcc tgctgtatca atcaattgtt gaataaagtc atcatcaata
     6901 gtgtgatcga tccgcatact tttttttatg ctatccaaaa ttaccatttt atctctccta
     6961 atgaaaaaaa ggcagtggga taaaccgccg cccaatattt ttctatactt ttggtgttac
     7021 aactggcgta aactcaatat acactgaagc attttgatca attttcttat aatcgttgcg
     7081 aacgatcact gcaagtcctt gtgagtagta atcgaatttt tcccattgag tcgttacttg
     7141 atttctacga gcaacaaaga tagattgtgc aatatccccc ataatcatag ggaatgttcc
     7201 agctttagga ttggcaaaca atgtatctgc aatcaatact actggcatac cgaataatga
     7261 tttaccacta ggtgctgtca catcgggttg taaaatgtaa cgtccatctg aatctttcaa
     7321 tgtatctagg tggtaatatc cgctttggtt tagaattacc attttattta atgctgggtc
     7381 taatgccaca ttgtatagtt gtttcaaatc gtccaacgta gcagccgttt tcttagtgaa
     7441 tgtttttaat aaatccatga tatgcttatt gtcagtgttt tctaccaatt tcgctaactg
     7501 atctttgacc tcttgtacaa tattcactgc tgaatcttcc acaacctcat ttgataaggc
     7561 aatcttacca gcacgagttt ctactttata atcaactgaa gtaaacattt cagcgtcaat
     7621 atcaccaatt tcagcaagtt cagcttttgt tgctaacact gcttgttggt tagttgctac
     7681 tggatattta ccttgtccat ttgatactgt ttttactgtt gcatattgag ctaagttata
     7741 atttgaacgt ttcaaatcaa agacttcacc gattacttct tcgggaacaa ctgccgctgc
     7801 atttacagta gtgactccat cacgcacttc gccttgtgaa cggatatatt cttcataccc
     7861 acgcaattct gttttttcgt tatcgattaa agttttttcc atgttattac tctccttttg
     7921 tgctttagaa gtggattcta agctattgtt tgtttgtttt gcttggttgt tactcataaa
     7981 cgattcgtaa gaacgcttat ttacttggac atttgatgaa tcataagcgg gaacggtcac
     8041 gacactaatt tcattaagtg ctttgatttt atctattgat cgttcaattg tgccatcttc
     8101 ttttttgtcg aatgaatcta atccaagcac gaacccaaag gacatagaat caacaacccc
     8161 ttttgagata ttctcataca catcatttga ataggtcgtg tcggttagct ctgcttcaaa
     8221 atgtaatcct gtatcatcga cattcaattt caacgtacct gctttgacac tagctagtgg
     8281 ctttgaataa tcgtgtccat ataataagaa cacatttgat aaatccacct cttttaatgc
     8341 ttcgggtgta atcacttcta caaagccgcc taaatctttt gacggttgcc cgaatttcaa
     8401 cgcatagcct gaaatagttt tgccgtctgt tggttgtttt tcattttctt tctctacttt
     8461 gggagaatcg gctgttagct cagcttcttc tgtcagccgt ttttcctttt cttcttcatt
     8521 attcaactgg tgtaactcct tcctgttctt ttaattgata tttctccaac gtatctaaat
     8581 acgtaaagtt caaacttgct agtaaacgat cgccaccatc catggggggt agccccattt
     8641 tcgaccgccc ctcattaatc gtgagaagtg accctttgac ctgatccaat acatttttta
     8701 cttttgtttc aggatcagtc tctagtaact gatcggaatt aaagcggata ttggtttcaa
     8761 gtttcgtatc taattcactc acaaacacat taaagtaatg aatcagcgta ctttggatat
     8821 actggagatt actttgaacg gtgctagaat gttcattttc aacgcctaaa cgctcaatcg
     8881 gtacaccaaa cgccttggca atttgttttg tgttccaatc attggaattg ataagcttca
     8941 aaacatcggt gtttatttct aatgttttgt agtccattgt ttcatcaaga atgatcgttc
     9001 taagagcatt atctccacta cttgaaccgt tggcttcttc aaacttttca cgaattgcgt
     9061 tctttgctga tccgtctaaa tctgacttat gaaccttcaa aatacctgac ccactgacac
     9121 cacgagtaaa gaaattgtgc aatgtgcggt tgccagcttg ttgaattttc agttcatcac
     9181 gtaacgcata aagtggtggt aatcctgtta aaccatcttg ggtgaaatat ttgaagtgca
     9241 aaatatcgct agacttcaca cgtctttttt tatcaccaat ctgataagac aacgtaccat
     9301 tatctaattg agtaaccgtt acacttgagt taggtaataa gtgaatttct tctacctttt
     9361 caccgttgcg tttaatctct gcaaagctat taccgtttag caacatattg accgccaaag
     9421 caaactttaa atgccaacca tccatttctg aattaggttt ctcgtttagt aacttcacca
     9481 gttcattatc agcttgtggc atattgtttt tgaccaattg aatcggactt gaagcaatat
     9541 cactagcaat aattcgaacg gctgcaaaca catcactatt tcttaatgca cgaacgcttg
     9601 taaagctggt ggtgtaatca tccgattgga ttgataccac atgttgtagg aaaggatctc
     9661 ccgttgtatt ttgtctcgct ttaaaaaatg ccatctttta acctcccttc tgttctgtat
     9721 ttagcatgac cgctacccca ataagcacta tgcccagcgc taacaagcca tagtagacat
     9781 ttgttagtaa ggtgatagca accaacacac acaccaatcc aagaagtaaa agaacggtat
     9841 gaatgtaatt agaaagaaaa ttcttcactt gtataaaatt cattgtctgc tttcgctcct
     9901 tccgttgtgg tgaagtaatc cattgcaaac acataggcat tgattaatgc agctattgga
     9961 tcaattttgt tactgttctt tgatttatta atctgaatgc cgttgttatc cgtttttaaa
    10021 atagcgttgt tcactgcatg ggtgagaatc gtatttttat tgtgaataac gttgccttca
    10081 taaacttgtt ctctaaaagt tcgggtcgga acgtttagag taatcgttcc ttgtcttact
    10141 tctaacattg ggtagttggc tttttcagct ttactgataa gcgaattagc gttatacggg
    10201 tcataacaaa tccccataca ttccaaatca ttttcttgaa tcaaatcttg aataaactga
    10261 aatacttggt catagtccac gattccactt tctaattgag taatggaaca ctcaccagct
    10321 ttttctagtt ctcgataagg caaaccgtcg cgcttttctt tgtcttgaag cccatatttg
    10381 gtggctacaa agctatgtga atcacaataa agttcgccgt tatctagcgg tacgatccat
    10441 gaaacgctag ttaaatcatc tgttttagat aaatccactc cgatataaac gggcttgcct
    10501 gtaatatctt gtggttctac ttcggttgcc tgccagtctt cagcaatcat atagctgtcc
    10561 tcactcgctt gtctccataa attgaagttt ttcaccaata cagaattaag gttattttgt
    10621 ttcaaagcaa ggttcacatc atcttgaatg gttggaatca ttactttttt gatttcttca
    10681 ctttcaaaaa tcggattggc tttgatccat ttctcttgat cgtgaatttc ttcgggatcg
    10741 tccaattccc atatcgctat aaagtacctg tcggcttgtt cttcgccttt tagcacacga
    10801 tctaacaata agtattcctc gtacatagga acgtttaaat caagcccagc ggtactaata
    10861 acacacaata agccgttctt ttgttgggtc attcctgact taatgacgtt gtacgtttta
    10921 cgagtttttg cttcgtgcca ctcgtcaaga attgcggttg ttggtgcaaa cccatctagc
    10981 gtgctggttt cactagccag tgccatagcg aacgaattag agggcaagtg ctgaatctta
    11041 gagttcataa ttttcagttg tggtcttaaa aacttactag acttgaccac gtttctaagt
    11101 gaattagaca gcatatcata gcccaattta gcttgtttca aagcgttact tacaaacaat
    11161 acttgccttg cttctgctgg ttcttgttcc ataatcaacg cattggcagc catgccactt
    11221 gcgatatacg ttttcccatt cttacggctc atactggtaa acgctcgatt gaatcgcctg
    11281 taattccctt ctttagtacg ccagccataa agactgccta gaatgaattt ttgaaatcct
    11341 aacatcttga ttgctttacc gtccgtagat ggtagcaact caatgaattt aatcgctttg
    11401 gtggcttgtt cttcatcgaa cacataaggg aagctatctg acttagaacg ctccaaatcg
    11461 cttatatgac gctcacacgc ttggataatt ttcttgggtg caatcagctt acctgatagg
    11521 actttctcaa tgtaatcatt catgaattca tcaactcttt gaacgggtct tcgggttcat
    11581 catttttaac cttgttcaaa gctagttttg atcgtgattc taaggtcata cctagtgagg
    11641 tggcttgttt tttcaaatca ctcatagctt gtgattgaac ccgaaccgct ggattggctt
    11701 tctttactcc gttttcgcat gtgataaacg tacctgattt tcttatctcg gcttcggctg
    11761 ttttcattcg agcaaaggct aaacaatagg ctaccaatga accataatct gcttcactca
    11821 atggataatc tttttttaat gttggtacga tacgattcca ttcacttcta gcgctcgctg
    11881 ccatccagtc agggggcgtg atatttatta atggttgctg cttgaacaat tcttctctag
    11941 cgtccacacg gattgctttt tcttcacttg atatattgcc ttttgtatcg tgtaatattt
    12001 tagggtgtcc catgttgtcc tcctttcaga caaaagaaaa aggaacgtaa gcaattaagc
    12061 tcacattcct tcgtttttcg atcagaattt ttctttatct aatttatcta tataaatgat
    12121 ttttccgttc tgcatttgaa tggtcacttg accaaaatga ggtaactcaa tggtgcgctg
    12181 tttccctgtt ttatcagtaa taacaattgt atttttcaat aaatctccta ctttcattat
    12241 atactttttc acttgtgatt acaagtgtaa tcgttatttg taattaccct tatgcctttt
    12301 cacaacggtt atttgtacca aaatgagtag gcatcgattg tcagtaagtt tcacactatg
    12361 cccccttaac tcaaagggta ctcttttctc gtctttgtct ttcattggct gtcttggaat
    12421 tatggtgtgc atggcataaa gaacgaaaat tagtttcatc taaccgtcta ttccaatcat
    12481 cttttagttc aattttgtga tctacaacat cagctttcct tatcaagcct tcttctaggc
    12541 aatcctcaca tactggatga tttaaccgat acaagtatga agcctttcgc catgttctac
    12601 tttgatagaa ttgaaaatat ttaccttcgc tttgctttct ttcttcacgc ttaattgccg
    12661 tttgctttgc tttatgcttc ccacaatact tctgcctata atcaatcaat gttttacatc
    12721 cagcatgatt acattgtttt ttaactgcca tagattacca accaaagcga tctcgttcgt
    12781 cagaatcatc ttgttcccat tttgatttgt attcaatata aggcgaaact atcttcaagc
    12841 acgttccgtc tttaaaatta actttagtga ttttatcttc ctcaaaacta ataaagtcta
    12901 catcttcttg agtgtttgaa tactcaatgt gtttctcacc atcgaagtat ctaatacttt
    12961 ccatatgcat aattattcta cctccaataa attagttatc aattcatcat ctgttagtag
    13021 caaagccaag aacttatcat ggatgtttaa aaacaaatta tcctcattcg atgtaagctc
    13081 tcgttgtctc gctcgatcat ataggttaag cagacaatta aattcatcca ttagctcacc
    13141 tgtatcatca tagttgccac tatctaccaa tgatcccact actagcgatt gacaccacgc
    13201 tactgactcc tgcagccaat caggttcttc ttccattatc tttaatactt ctggatcaaa
    13261 tgtggaataa tcttctgtta ccaatcccac actaaatgcc agcgtgttca ttagaaaccg
    13321 atccatttca tctgtatatt gaaagttcgg tgctttgttt gttgaaataa tattgagcat
    13381 ttactttcct ccttgattcg taacacgtgg taacacctac gtaacaggta cccgttacca
    13441 ctcaatccca acggggacaa gggttctagc gttttggtaa caggtaacgg gtaaaatcca
    13501 gtaacattat tccccagcat attagctaac tatttcaaaa gttttaagga aataaggtgt
    13561 taccccgtta ccaaattgct gtatcccttg gggcagtaag ggtaaactag taacgggcta
    13621 ggtgttactg acccgttact acccgttact ttttgtactt tccagctcga tttttatttt
    13681 cataggcatt tgacttattg taaaatcctc gtcgtttatt tctttttggc agttgcttcc
    13741 gttcccaacc aggaatactg ctaataatca aggcaatctt tttagtttct gcatctgatc
    13801 gaccattcaa taaatccttt gcttgtttat caaaaacagc ttccaaaata tcagcggtga
    13861 gtacgctatc aactaaataa aactcttgtt gcatagtagc tccattatag aaatgctgta
    13921 aatttttctc attacattta ctgtcaggat agttctgata ataagaacgt ctcgcccacg
    13981 ctggagcttc ttcccattta tacgggattt ccatttccaa atatttagtg attgcttctt
    14041 taattggatt ctccatcttg gcatcctctt gatagtcttt tgcaatttct aagacttctt
    14101 tatcttcatc gggactaaaa tagattcgtt gtcctttatc aaaaagcacc tttgcttcag
    14161 ctaacacttg aagaaaatat tcatcctttg ttttaaacgg attcatcttt ggcttattct
    14221 tacaaggcaa aggataaaaa cggcggtttc ctgtatcatc tttcaaatac tgcccatcat
    14281 tcgacgttcc aataaataca cattgccttg cccaagctat gacattccga ccataaggag
    14341 gtctaatttt atcgatcttg gcactgatga agttttttat ctgttcaatt ttagttttgt
    14401 tcattgaaga taattctcca agttcaataa ttacattgcc ttgaagctgc atataatcat
    14461 ctttcttttc acctagcgaa tctaatgaat cggtgaaaaa atcatccgtg taaagtaatg
    14521 ctggaacggt actcttacca atcccttgcg gtccatccaa aactggaacg atctcaaact
    14581 ttattcctgg tttatagata cgtgcgactg atccgactat ccaacgtttt gttacttctc
    14641 gtgtgtaaag gttgtcgtct acccctaaaa ggtcaataaa cagtgtttcc agtctaggct
    14701 gttgatccca tttaactttt tcgatacgtt cttttactgg gttgaaaaca ttctttctag
    14761 ccacatttac tactgctttg tgtattttgt tgtcatcaaa aactacattg tagttttctt
    14821 caatatatgc cgttaaggca tcctcaaaac tgtcttccca ttctccagcg gtacgattga
    14881 tccaaggaga atcttttaat aggtgaatgt atccgctaaa ttcgttataa gcaattgttc
    14941 cagctaaatt gctatcattg gttaaaatca attctgcatt cacaacactg ttacttttaa
    15001 tctgatcgtc tttattgcat cttaatcgtg acatccaggt tttcaattca atgatattgt
    15061 cttcacgctc gctggcttcg ggaaagtcta aaatactatc taatttatgc ttatgagcca
    15121 tgagctttcc ccctcaaatc ttttttaatc atcgatcgaa taatttgatt cacttcttta
    15181 tcttttagcg gtggttcatt gtacgcattt gctgtataaa tcaattctgc taaagtttga
    15241 ttatctacac cgtgccatag taaccaaccg cataacttag ttaaatgatc gtttctacta
    15301 ccttcgccag cgccttgaac aatcatatct aatttttttg ctgtccctgt ttttgctacg
    15361 ggtcggtatt gagtctttat ttcttgcggt ttttttagca gtaggttcac tagccaatcg
    15421 ggagctggtg caatattatt tagagatacg ttttcaatag gttcatatgc tctcccaaca
    15481 cttcttgaaa taatcatagt tggcgctatt ggaactcctt tagtgataat atcaatcccg
    15541 cttaaacttt tacctttatc atcacgcccg aagatatcag caaatgcacc tgtttcttgt
    15601 tggataatta caccatcagg aattttaaag aagaaatgga ttcctttacc tggtgtgatt
    15661 tccgtataag tagaaggaaa tggatcatac attgaagcaa gttttttata attctcaacg
    15721 ccgtttcgtg tttcagaatg ttgatctagg tcaactacca ccatttgatg atcttcaagc
    15781 attaacccaa tatttctagt tgcatcttcc gtccaccatt cattaatttg attagaatcg
    15841 ttagttgcat cattccaacc actagagcct tttattggaa ttttagttcc aggagataag
    15901 ggataaattg accatcccga cttttgtaat tccgttgctg cttgataatt atttgccaat
    15961 aaacctctcc tcctttttca aaatactggt ataaatcatt cagacacctc catgagcttc
    16021 aactgctcaa gttcgtattt caacccatcc cgaacaaatt cagcaccttt taattgattc
    16081 tgatacttta cccatgtatt aaatgttttc acccttgctt cttgcatatc agtaatgttt
    16141 aaccatgcgg ttaaaatctg attttttagc tcgttgattt ctcgacaaat tattttgtgt
    16201 tctgcatgtt ttttgagtac atacaaaact gttagttttt cattgaatga atttccaaag
    16261 taggttttcc cgatattatt ttctagttca gcgatccctt gattaatttc ctctaattca
    16321 aggctatcga gttggtttaa tagttttgtt ttgtacatct acttatgccc tccagttctt
    16381 aattgatagt cgtactcgtc ataagcagtg aagagatata acgctaatcc tgcaaagata
    16441 gctatttttg caattaatgg taataagccg aaaattccgc cgattaaaat gcttcctata
    16501 attaagtttc gtttcataat ggttccctcc gattttttaa tatggtataa tcggaacaaa
    16561 gaatatcggg ttttatccca attaagttgc ctttatcctg tccgccaaga ctgttatgga
    16621 taaggctttt tctttgttct cgatcatgtt aatgctccta agttgtctgc aattcagcag
    16681 aacgattata aattttctca ctcactgaaa actgcaaccc atatttttct ttaacagtca
    16741 ttaattctac tgtctcatct aaaataggtt cacgatcaac caacatttca ggagtcatat
    16801 ctttcttttt taccattttg gaatgaccat aaatagttga tacagcttta ttggctatca
    16861 cattcgcttt aatgtagtct actggttttg gttcagtaat agcgtggctt aatttagtca
    16921 ttgcttcttt ttggtgttct ttgtctagca tgcggaatac ttgaaagcct tcaagtcctg
    16981 tggactggcg tagttctttg atgatgtcga aaatccagtc ttgaaatgcc tcggcttctt
    17041 ttttacggct tttgaatgcc atgcggtaaa tatttttttt cgttgatgat gtttacatct
    17101 tgtgttctgc ccaatgaatc gatgacctta ctagtagtaa ccccatcttt aggcaaagag
    17161 tgaatggctc tagtaacctg tttaagacct aacgcttccg caacatcttt cgccactgcc
    17221 caccattcat cattaatatc gacaaatctt ataatgtgtc cgttccaaat ttcagttttc
    17281 attcaacgtc ctccagttca agccctagca agctcgctaa caactcaact tgctgataca
    17341 aaatttcttg atactcttca atagaaagtc gttctttaaa ctctgcttgt actaaatcgt
    17401 tcaagccacg caatactccg cttaccgttg gttcttgtgg tagcaatttt tgatagtcca
    17461 tttgttcgta caaacattca aacgtttctt ctagcgcttt gccttccaag tttgctaatt
    17521 ctgccgctgt gtcttctgta agaattgttg ataatacttt ttcgattgct ttattttgtg
    17581 tcgattccat tattatttcc tcctattaat ccctttgccc ccacggcttg cgtttatttt
    17641 tggtattctt ccataaagcg atcacattca tcttgtgaaa ttcttacgac tccatcaact
    17701 ataatcaact ttaaaccgtt aggaacatat ttctgcatca tagtattgta tgaaacgttc
    17761 atatacttag cagcttgtgc cttgttcata tattttggaa actggtactt tttttctaat
    17821 aatgaaagta acgttccttg agtttgctct acttgttgaa caactcgatc gtcaataatt
    17881 ttaaaagctg gatcatcaag cgtgagtttg tcaattttta attccatatt tatcccccct
    17941 cgttagcata catttctata caaagatttt aaaaaaatgc aagaggctct tgaatacatt
    18001 tctatacctc ttataaactc aatataccat catacctttt catttgcaag aattttaaca
    18061 tacttttttg aacttttatg tttcacacat atttaaatcg tgatatagta gagaaaatat
    18121 atgaatggtg gtgaaaatgt ggctagaaaa aaataccccg aagcagtgga gacaggacaa
    18181 agaattagac tggctagaat tgaaaaggga ctctcaatgg aacaacttgg aggtaaatta
    18241 tccccaccgg ccagtaaagg tgccgttagt aattgggaaa atggctataa tcttcccaat
    18301 aacaccaggc ttaaacaact ttcaaaggta ttagatgtat ccactaccta tctattaaac
    18361 ggatcttata cattaagaga tatgcatatg atgcccgaaa ttaagcgaaa tgtgcttgaa
    18421 aagcaattga aaaaaactat tgaaaaaaat aactctataa ttgattctaa tctttctaat
    18481 gctatcaata acatcgaaga agataacctg actgtctcac agaagattta tttaacaaac
    18541 gcaataaaat atcttaaaac aggatctcga attgaatttc ctgacgggag taccccaatt
    18601 gcttatctcg cagctcttct aaatacgctt ttacgatatg actcattaat taatgattca
    18661 gaaaaaaata cagatgagaa aaaagagtat caagaatttt atgaaaatga tcttctaaaa
    18721 gagttcacag ctcttgttac tgagctaaat aaacactatt caaaataata ataaactgcc
    18781 tcctccaacc gcccccacgg cttgctacac tatggaggga aaactattat ggcaacattc
    18841 aagcaatatg aaactaaaaa aggtaaattt tggctctatg aagcctatct tggaatgaac
    18901 aaaatgactg gcaaacctga taaggttcgt agacgaggtt acaaaacaaa aaaagaagcc
    18961 cagcttgcct tgtcacgctt gcaagtggac tacgataaaa acggtattaa aaaagcgaac
    19021 aacgaaacgt ttaaagaagt ttatgacttg tggctagaaa catataaaac cactgtccga
    19081 gaagccacct tcatgaaaac tgaaatcaag tttagaaagt ggattcttcc aaaatatgga
    19141 aatcttcgtg tgaatgaagt cacggtaaaa aaagcccaac aaattgtaaa tagctgggct
    19201 gaaactactg accaatacaa ggtacttcat tcaacttcta agcgtatttt tagctacgct
    19261 atcaatcttg ggattattga gactaatcca ctagacaacg tattaatgcc taaaagaacc
    19321 accactaagc ataaagaaaa ggtcaaagtt tacagtaaag aacaactgat caccttgttt
    19381 gattacctaa acagcaaacc tgaaacttat cgaaacgact ttgataaaac gttactacgc
    19441 tttctctttt atagcggggt taggatcagt gaagcccttg cattgaattg gtcagacatt
    19501 gattttcaag aaaatacggt caccatcaat aaaacactaa gccaatccaa aaatggctat
    19561 aaaatcagtg atcctaaaac tgattgtagt gctggggttc ttccactaga tgaaagaaca
    19621 atttctatat taaaaaaatg gcagattaat cagcggaaat atatgctgac acttggtgta
    19681 actgatccga ctatgatttt ttgtggtatc tataaagaac taatcacaca ccacgctatt
    19741 tatgcaagat tgattactat ttgtgaaaaa gctatggttc catttctcgg tgtgcatgta
    19801 gcaagacaca ctcatgcttc aatgctcttg gacgctggag ctactatggt agaagttcaa
    19861 actagattac ggcatacaaa aattagtcag acgatcgatt gttacggtca cttggcaaaa
    19921 gaaacgaaag aaaagactgt tgaaaaatta gtacaacact taaatattga ctagatggca
    19981 tacaatatgg catacagtaa aaaataaaaa aaggagaaac cctatcatat caaggtttct
    20041 cctagcttta tagtctattc ttcttcttcc gccatgaatg tattgaatac ttcttcaatc
    20101 atgtcccatt ctgcgtctgt ttcgatttgt tctaactcgc cttctgttcc atcttcgttt
    20161 tcgatgtatg cataagcttg taattctaca tcttcatctt ctgaaacacc agcaggatat
    20221 aaaagaacat agttacgtcc aaattcttct tgtccgtcaa ctgttagtag gatttcatat
    20281 aatgtttcat ttccttggtc atccaccaat gtgatgtgtt cgtgtccttc atggtcgtgg
    20341 gaatggttat gttctgtcat aagattacct cttttctttt attattaaaa taattgctta
    20401 cctgagccgt ctaaataatt ttgtaagatc atcacagctg ctaatttatc gatgactttt
    20461 tttcgtttgg ctcttgaagt atttgcctgc tctactagca tacgttctgc ctgaacagtg
    20521 gttaatcgtt catcttgata atcaactggc aaggagaata gttctttgat cttttctccg
    20581 tatgacatgg aagcttctgc ccttggtccg atggaattgt tcatattttt aggtaatcca
    20641 actacaaacc ggtctacttc atattgtgct actaattcct tcaaccgatc aaagccaaac
    20701 tcttcttctg cttcattgat ccgaatgatc tctactcctt gagctgtcca gccaaagggg
    20761 tcactgatag caacacctac tgtcttagaa ccgacatcca agcccattac tctcataaat
    20821 tcactccatg atttgagagg tagtccttga caattacttc catgatctca tcgcgttcgt
    20881 gacggcgaat caaatttcga gcatcacgat aacgaggaat gtaggccggg tctccagaaa
    20941 gcaagtagcc gacaatctga ttgattggat tgtagccttt ttcttccaaa gcccgataaa
    21001 caatcgctaa tgtttcgctc acttcttttt tgcgactatc gtcaaaacca aaacgtacgg
    21061 tttcatctgt aaaacccatg attctacacc tctttcccgc aaggtaattc cagcttacct
    21121 tctacaacct tattttactc gaagaaaggg aaaactacaa acaaatattc caaacttccg
    21181 aataaattaa gaaacactat ttgtatgttt ttttcctttt ttctgtagct ttatcaaaag
    21241 tactcttttc ataaagtata cgctatattt ctttgaatgt atgtttttac acaatttttt
    21301 cataatattc ataaaaaatt cgaatagctg ctactaattt tttgtttttg ttgcttgtcc
    21361 tgtctcataa ttgatcgtgt agccatcttg aacattgtag ataaagacat tgaattcaat
    21421 ttttttgtct tcaatgcttt ctgcttccaa ttgcactccc ctggctacca gttcattccc
    21481 ctcaaaatga ggagtgacac ggtagcgtac atgatgattt gtttctttga tatatgccgc
    21541 aatatcattt tcgtgagcaa gcatgtatgg agcattcaac gaacgtgtcc ctgtcatcaa
    21601 gtttttaatg ttgttatttt gaccagtcag ctggaaacca attaaatggc tgcgattata
    21661 taaccattgc ccgtcactta atttcttttg tttccaacct gtcgggtcaa tatacaaagg
    21721 ctctctcttt tcagtaggga acaaatcttt tccaagcata gcattggctt gtcctactct
    21781 gttcaattta tcaatatttg aatagctttc ccatgttcct ttggaaaggc taagatcctc
    21841 ttttgtaaat tccggctgat tcccattgat ttctatttct tgatgttttc catcatattc
    21901 tggcacatcc gctacttgag aagcttgtgg tgtggaatca aaaatagttg taatctgctg
    21961 aaagatatct gaatcttttt gtgtggtgga aacaccgaat gcccctgcaa tcacagcagc
    22021 gacgatcccc acgatcgcct tggttgtttt tttgctcatt tctttactcc tttactaaca
    22081 gctccctaaa tcttaacata tgttccccta taaataaata ctagaaagaa atagaaacag
    22141 caaaaaataa aacctgtttt aagatggctt aaaaatacaa aaaagagacg gattcctcgt
    22201 ctctaatcag tcaccactct atctgtaagg tatttattat tattcaaaaa ttttcttcat
    22261 actcaagaca atacactgat ttcacaaccg cttggtaaac ggtgcgcaaa agcttgcatc
    22321 tgcggtaata agtccatcca agttaaaaac ataagaagtt gtttttgctt ggctgttctt
    22381 attacttgtt gcagagcagc tttttcacaa cctattttaa gcgggtgacg ggaatcgaac
    22441 ccgcgacatc agcttgggaa gctgggattt taccactaaa ctacacccgc aatacaaaaa
    22501 cagtatacgc ctcttttatt ccttcctcaa gtttttttat tctttacgct tctggtatgt
    22561 tttgtagtaa aataaaacta atcaatcaat tattggagaa gatgctatga cgggaaaaga
    22621 attagatctg ttgctggaca cttgtttact cgctgggaaa atcatgatgg aaagcaatgc
    22681 ggagatgtat cgggtagaag atacgatgag tcgaattgct ttagcttctg gaaatttccg
    22741 tttggtcagc tatgtcactc aaaccggtct ctttattggt cttgatcgta cctcgaccat
    22801 ccgaatggaa caaattacca accgttcgat taatttagaa aaagtagtaa aagtaaatga
    22861 tctttcacgt aaatatgttg cttcagagct aacattagaa gaactttact gcgaactgca
    22921 agcaatcgac cgagaccgtt ctttctttcc tgtctggttg caaatcgtta gcgcggcagt
    22981 gatcagtgga acaattatgg tgttatttgg cggaaagatg accgatttac ctgtgacttt
    23041 agtcattgga ggattgggat acgcagctta tctatatagt ttgaaatttt ttagaatcaa
    23101 atttttatcg gaatttttat cttctttatt gattggcata gccgctatct acagcgtacg
    23161 ttttggctta ggaagcaatc aggatctaat cattatcgga tgtgtgatgc ctttagttcc
    23221 tggagttcaa attacgaacg ctatccgtga tttgctagct ggacattatg tctcaggagt
    23281 ttctcgcgga acagaagcga tgatgactgc tacgatgatc ggttttgcga ttgcttttat
    23341 tttccagctt ttttattagg aaaggaacac aagtatgggg acgatattta ttcagttttc
    23401 ttttagtttt ttagcgactg cggcatttgc cgttattaca aatgtaccta ggcgttcatt
    23461 gatttgttgt ggtctttctg gtgcttttgg ctggatgatc tactggatct gtgttcaaat
    23521 aggcggatct gctgcttttg gttcattgct tggttcttta ggagtagcat tcatcagcga
    23581 tcttttctct aaacgtttga aaatgcccgt aacgattttc aatattcccg ggatggttcc
    23641 tctagtccct ggtggtttag cttatcaagc ggttcgaaat ctcgttactg aatcctatcg
    23701 agaagcggct cattatgcgg tccaagcaat tatgattgct ggagcaatcg ctttaggtct
    23761 tgtattatca gaagtcttta accataacat ccgtaatttc aaagtaaagc gtgacgcaat
    23821 catgatgaaa cgaaaaaaac ggctttttaa ataaacgaag tgtaacaaag aaaaggttgt
    23881 gaaagagacg ttcagccttg agcgatgaca aaatatctca caaacattgc gacaagtaac
    23941 cgcaggagca ataagaagta attatgagta ttaaaaaaag ttttgtaaaa atagcttctc
    24001 acatttttac ataaaattga cttaatactg agaaactgac ttttgaacac cctctatctg
    24061 taaaaaaggg gctgcgacat gatttttgtc acggcccctc ttctttattc aaattttttc
    24121 caaaacgttc gttgataata aacgatccat agagcggaaa gtattagaac taaaccgatc
    24181 acaggaatat tcactaacca aaatggataa aacaaagcca acattccgta taagacgata
    24241 agaataatac tgatgatcaa tgaacccatc atagttgcga ctaaacccaa acttcctgaa
    24301 cctctgttaa ataattggtt aatattggtc cagttcagca gcagcaaccg ataatctcta
    24361 gcgaaataac gtagtgacaa taagtagcag ccgaataaag aaccaaatag caagctaggg
    24421 ataaaaaaca aaggcagctg aaaagacagt ccgcttaata atgcgatccc accagttaaa
    24481 aaactctgca atatccatcc gaatctgaac ttttctttta aatattggtt catagagatt
    24541 ggcaaagaac ggataaacag aaaattttct tgatccaaag aaataagatt gctgataaaa
    24601 gaagtctgat taactgttaa gaaagccaat gcgatcccta ccaggaaaac aacaccgatt
    24661 aacttattat ctaaatcagt taattttatt tcgcctccta gggcaaaagc aagaataaaa
    24721 atgattggag ttaacaagga agaagaaaga acttgcataa tcaaattagg ttcttttact
    24781 aattgagcat tgtaactaaa taaaagctga tgcacatttt ggttcttttt atgggttctt
    24841 tttattttga actcactgtt tgaatcagtc agttgctctg taagctttgg tactatcagc
    24901 tgttttacag caaggaaaaa tcccagagcc gtgatagcta gtattcccca acccaataaa
    24961 ccttgttgag aaagtggctg actggcagca tcatagaatg gcaataatat cgaaatcgga
    25021 tgccgatctg ttaactcatt ttcaatagtc cccgtttgat gattcatcca catgattcca
    25081 gcaaatgcga tcagcatcga gctgccaagc aagatactag aaactgtttt tctatgtttt
    25141 ttaaacactg ctgttcgagc caaaccaaaa acaatcaaac tagccagaca aaaaagaata
    25201 atcaaaaata gagaaaacaa aaggattgag ataatgatag taagcaaaat ccctagatca
    25261 gctcgccatc cggttaaaat aaacatgact agaagcggaa aaacaaaagg aatgatggtc
    25321 aaagcaacta ccaaaatctt tgctgaaaaa acctgttctt gtccaaaagg cagaggcaaa
    25381 aatacttgca ggtcatttcc ttcaaaaaag atattataaa tgactgatat actttgagtt
    25441 aaacctaaca taccaaaaag tgccacgtaa taagtaaaga aacctggcaa tttaacaaag
    25501 ttcatagcaa acatagcaaa tccgtaaatc agtaaaaaaa tcagccctga aaaaagattt
    25561 tgagacaaaa gatagatagt taatcgcttt cccttttttc ctttctctct tgctttattc
    25621 gtgagctgtg gattaacata taaaagatta actcgaatta attctaataa ttgtcgctta
    25681 tacatacggt ccatctcctg tcgcttgcct tccagccatt ttcaaataaa tcttttctag
    25741 cgtttcatca ggtgagccat tcaataattc actaactgcc ccctgataaa tcaattcacc
    25801 tttcttcaaa atagccagtt tatcgcataa ctgttgtgcg gtatccaata catgtgtaga
    25861 aaaaatgact gtttttcctt ttgcggcatg agccttcatc atttctttca aatcaaatgc
    25921 tgcttgagga tcaagtcctt gcaaaggttc atccaatacc caaatatctg gatctggcag
    25981 caacgtgccg atcaaaatcg ttttttgacg cattccatgt gaaaaacttg cgattgtttc
    26041 attttgatga ctatacatat caaaaagcgc tgtcaattct gctaaacgct tttctttttt
    26101 ttgtgtgtct aattcatagg cagctgaaat caagtcccaa tattctccag ccgtcaattg
    26161 taaaaaaata tctggggaat caggcacata accgattttt tgtttgatac tcaatcgatt
    26221 ttccgtcaat ttcattccat ctactaaaat cgttcccgat gatggttcaa taatactaac
    26281 taaactttta atcgtagttg attttccggc accattatgt cctaaaaatc caaaaatttc
    26341 cccttgtctt atcgagagag acaagtcttt taatgcttgt tttttcccgt aacttttact
    26401 tacattgatg aattcaatca tttctgtact ccttttgttt gttattactt tttattgtac
    26461 ccttcgttat tggtggagta aatgtcaaaa agagtgcata acaaaaaagg atagaaacga
    26521 ctaggctgct acttgggttg ttctcttcta caggaaaaca ctcttctaac aggtaagata
    26581 tgagtctttg ttttctttaa aatgattcat tttctatctt ttcttgcata aaattcttta
    26641 gtttatcaat acttctcttt cccatctcta gttgttctcc atttgcaaat gttactattt
    26701 cgtttactct gctaatagat gtaatgtttt cagtattaat gatataagct tttaaatctt
    26761 tacataaata ctcatattcg agtgattttt tatatttttt aattgtgccg tttacgagaa
    26821 tctcttcatc tttcaatact aggcctattt tatttttata acctggcatg ctcattagat
    26881 aaaggatttc atcgtaatgg atatagtgat cgctattacc ataacggaca gttgtccacc
    26941 tttttttcgt agcatgttga acaggatagt tttctttttc tttcaataag taactttgga
    27001 tgcttttttt atttttttca taagtgaatt cttttatcaa ataaccacta ggcgtaatct
    27061 ttcgattaat aacagttatt gctttattcg gatgagacgt tagataaaca attttacact
    27121 tttcattctt ttctctaagc atttcgccta gttcgatacc gctataatag gcgttcaaat
    27181 cgatgtctag gaaaaaaata tctgaggaat agatttcatt atttttaatg aattcaacaa
    27241 attgtataac gttcaatccc tgaacgatct caaatttcca aggacttaat aaaattctgg
    27301 attcttccaa aatttctact agcatttttt caaaaaccaa actatcatcc aaaataaata
    27361 tacgcgttac cataaaaagt acctccactc gatattttac tatcaaaatc atttcataca
    27421 acttttcaac atacatgtta ggaataaatt gtaattctct tcacaatctt ttccaacacc
    27481 tttgaaaccg atatcaaata ctttcaggat gctgcttcta aaataaatat caaacaaatg
    27541 aatacatcat gcaaaaaaag ccagacaacc gacgtccttg cgtgtcagct gtctgagcta
    27601 tgtttattta ttttgttctt ttaaaaagaa cgatacacat actgattttt cggagcagag
    27661 actttctcag cgtttgtaac aactacagat gggatttcac gcttgaatgg ttgataatag
    27721 tctgattgga tggttccttc tattttatac cattcatcat ttttaaccgt tactccttcc
    27781 ggcatatgcg tcaacaaacc aaagactcct gaatctgcca cacaatggat aatgccgaat
    27841 cggaacaaga agatatctgt attgctatcg ttcggtgttt ggtaaacaaa tccttcatag
    27901 ctgatttttt tgccaatgaa ttcgctaggg taattataaa ttagctccat aacttctaaa
    27961 tagttttcat ctgtgatctt gatagtttgc ttatccccat acttttcttt caactcattc
    28021 atttggtcta agtagtctga tttgttgaaa taaatactgg tatccggctt taagtattgt
    28081 gtctgcatct ccggatcccc tactgattct ttacttactg ggaaattaaa tcctttggct
    28141 tctacgatcg tagtgtcgag actaactgtt ggaaacaacg tccccacgat tactggcaaa
    28201 gctagcagta cataagcgat tccacgttga taaggttttg acaacccatg gtcatgatcg
    28261 tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc acaaatacag ctgaactaaa
    28321 gccaaaagag cagataaaat cattgaaaga atagctaagt agcgataatg gacattgata
    28381 tattgatcga gtttgccaga aacttgcaag tacatcatca attcgaaata cccagataaa
    28441 atcaaaaacc gcagcatatt ttccctccta aataaataat gtgaatccaa taacgctaag
    28501 ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta aaatatcgtt tcatcatcag
    28561 tagatttttg atatcgacca ttgggccaaa gaccaaaaag gcagctacag gtgctgtgcc
    28621 gaacagactt aacaaagaag atcctataaa cgcatctgct tctgagcaaa gagacatcgt
    28681 aaaggccaat aacagcatga tgataatggc taaaatcttt gttgagccga gcgttagcat
    28741 aattctagtt ggtaaataag tctgcatcgc agctgcgatt aatccgccaa tgatcaaata
    28801 acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac acatgcccag tttgatggat
    28861 cagctgattt tttggttcat tcttctcact ttgttcttct tcatggacat gatgatggtg
    28921 attttctgct actttagacg tgattttttc tttcaataca gcgtcttttt gaaaataggc
    28981 aagccagaca ccaatcacaa gggcaacaaa aaagctgcct atcacccgcc agcttgccat
    29041 ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc acgattggat tgatgatcgg
    29101 agcagtcagc ataaaagcaa acgctgtgtg aactggtacg ccttttttaa caaattgatg
    29161 aacgatcggt acgatgccgc attcacatga aggaaagaaa aatcctaaga tcgaaccagt
    29221 aaaaatagag aagatacgat tttcaggaag ccatctcttc acacgttctg gggtcaaaaa
    29281 tacttgtaag gcgcctgaga taagacagcc taataatacg aaaggtaatg cttcaataac
    29341 gatcgataag aaaatcgtac tcatctgcaa aaccgaattt ggaataaaag aaaacatgag
    29401 tttcctcctt tttatttcta gttcattcgc tttgcaaata tacgctttta ttcaaaaaaa
    29461 acaagttgct ctgtaaattt ttacacaact cttatttttt accaaagcac ttcaattgac
    29521 tttttctgtt attcccatta aattaaggtc tcatacatat cacttctatc ataaaaggga
    29581 gaataaaaat gaaacaaacc actaacttga ctgaagttca agacatactg caacagtcat
    29641 ccgttgccgt catttatctt tcgatgccta attgcagtgt ttgtcacgct gttcgtccac
    29701 gactagagga acttctcaca cattatgata ttccggcact tcatttggat gcatttgaaa
    29761 caccagaagt agctagtaaa tttgaagtgc tgactgcgcc agttgtcctg atttttcatc
    29821 aaggaaaaga agtctttcgg caagcacggt tcatcgactt taaaaaaatc cgttatctac
    29881 tagatgaact gacagcagaa gatgattccc tttcttatga agaaatcttc cgaacaaaat
    29941 aatcgttgtc caaacagaaa aaagcgattg aacaagtgaa agttttgcct tcatttgttc
    30001 aatcgctttt tatttttatt ctgctgtatt ttcggaatgc gcatttgcgt cagaatgcaa
    30061 gaaatagatc aaggtttgca attcattcgt caaatcaaca ttttggatca atacatctga
    30121 cggagcattc aatcgagcag cggtaaagtt caagattcct ttgattcctg cttccgttaa
    30181 ctgattgact acttcttgtg ctctttcagc gggtatcgtt aaaattgcta cttcgatttg
    30241 ttggatcttg atttgttctg acatatcttc cattggataa acggggatac cgtctacgat
    30301 gcgcccaacg atttcctcat tcacatcaaa agcacaactg atacgtatgc tattactttg
    30361 atggaatttg tattttagta aagcacttcc caagttccct acaccgatca gcgcaacatt
    30421 tgtcaattga tcgtcgttta atgtctttgc aaaaaagtgc ataagatctt ctacatcata
    30481 accataccca cgttttccta attcgccaaa ataagaaaaa tctcgacgga tcgttgcact
    30541 atctacttga acggcttcac ttaattcagt tgaagacact tttttctttc cagcattatt
    30601 taaaatcctt aaatagcgat aatacaaagg cagtctttta gctgtcgcct ttggaataat
    30661 atgatctttc acgaaatgac ctccacgtat tgtctaatta catctttgat ttctccatca
    30721 tagcagtatt tataggaaaa aatcaattcc attgctcaga tattgtgaaa ttttattctt
    30781 tattcattta tttatcattt ctgcaaatct cgcagtataa tggaatttat ggtagactac
    30841 taattgaaaa atagtttttt atttgaggag aattcacaaa gtagaaaaga ggattccatg
    30901 atattattac aagccaatca gattgctcgc cattttggtg cagaagtctt atttgaaaac
    30961 atacatttag aaatcagtac aggcgctcgt gtcgctttag ttggcagaaa tggcgctgga
    31021 aaatcgactt tattaaagat cattgctgga attgaagcac ctgatgaagg aaagatcgct
    31081 aaaaataaaa cagcgactct cggatacctc gctcaggaca caggacttga ttccaatgaa
    31141 acagtttgga acgagatgct gaaagctttt gaacaagtta gacaaatgga agagcggatg
    31201 cgcgagctag agattttcat tagcgaaggt acgccagaca cacctgctta tagttcttat
    31261 ttaaaagaat acgaccggct gcaacacgat ttttcagatc gaaacggcta tggttatgaa
    31321 aatgaaattc gttccgtatt gcatgggttt aaattcgatt cttcatttta tgatcagaaa
    31381 atcgatactt tatcaggcgg acagcgcaca cgtttagctt tagctcgcat gcttttgcaa
    31441 caccctgaca ttttgattct agatgaacca acgaaccatt tagatatcga gacactttcc
    31501 tggcttgaga attatctgca aagctatact ggtgcattat tgattgtttc ccatgaccgt
    31561 tattttttag ataaagtagt caatgaagtc tatgaattaa gcagaagaaa aatgcgccat
    31621 tacaaaggta actattcgaa atatctagat ttgaaagcag aacagttagc aagtgaatgg
    31681 aaggcttatg aaaagcagca aacagaaatc cataaattag aagacttcgt tgctcggaac
    31741 cttgttcgcg cctctaccac aaaacgagcg caaagccgca gaaagacatt agaaaaaatg
    31801 gaccggctgg atcgtcctca aggggatgaa aaacaagcaa actttctatt cagtatagaa
    31861 aagccttctg gaaatgtagt cttgcaaatc gaagatgccg ctatcggata tgataatgtc
    31921 accttatctg aaccaatcaa tctagatatc cgtcgtcaag aagcgattgc tctagtaggt
    31981 ccgaatggta tcgggaaatc gacgttgctg aaatcaatca ttcaccagat cccttttatc
    32041 cgtggaaaag aaacgctagg gacaaatgtc accatcggct attacgatca aggtcaagct
    32101 gatctgcata caaacaagac gatcctagca gaattgtggg atgagcatcc taccactcca
    32161 gaaaaagaga tccgaagtgc tttaggtgct ttcttatttt ctggcgaaga tgtagaaaaa
    32221 ccaatccctc tgctaagtgg cggtgaaaaa gcacgggttg ctttagccaa gttagcgatg
    32281 aacaaagaaa attttttgat tttggatgaa ccaacaaacc atttagatat tgacaacaaa
    32341 gaagtattag aaaatgcatt gatcgattat gacggcacac tgctttttgt ttcccatgac
    32401 cgatatttca tcaatcggat cgcgacaaaa gtcgtcgaac tctctgaaaa tggcagcaaa
    32461 gtctatctag gagactatga ttattattta gagaaaaagc aggaagaaga agaaatcgcc
    32521 gaattgttgg cacaagatga gccagaatct gctcctgctc ccacgaacaa atctgattat
    32581 taccaaagca aagaacagca aaagcttatc cgtagccttc aacgaaaaat tactcaggtt
    32641 gaagaagaaa tggcgcggat cgatgaatgg atcgatcagt tggaacaaga aatggtcgcg
    32701 ccagaaaacc tggaagatca cgtaaaactc aatgagctga accaagattt agaagctgct
    32761 agacaagatc aagaaacaaa attgacggaa tgggaagaac taagcttgga attagaagaa
    32821 tatgaaaatt aacgaaaaaa ccaaaacaga aaccattcat gctgttttgg tttttctttt
    32881 tttattttat caaacaaaaa ttccatattt acctttttca ccaataagaa gaccaattac
    32941 tagtcattct cactaaagct tctaaataat agtaatcacc ccaaatacaa ggtgtattta
    33001 ccccattatt tttgggctta ttataaacag actgtgttaa aaagccatct tcttcttccg
    33061 ttgtagcata tctttctttc aattgtcgca taattttcca aacagcagac tggtataagt
    33121 tcttttctgt tttgtctaat agacctgatt ctattaattc taataatcca caaaccatga
    33181 tcaccatact agatgtatct ttttcttctc tgctagaatc atcaaagatc aaatcccaat
    33241 aacaaatggt atcttctggc aaccgattca taaaataatt ggctaatatt ttgcttaaat
    33301 caaaatattt ttgttctttc aaataacgat aacttaatgc aaaaccgtaa atcccccatg
    33361 cttggcctct agcccaacag gaattatcac tataaccttg atgcgtactt ccatatcttg
    33421 gttttccatc tttgacatcc atataaaatg tatgataagt tgaaccatct tctcttacta
    33481 aataatctgc tgcattttga atatgagcct gagcagcttt tttatatttt tgatttccag
    33541 tttcttcact cgcccaatac aataatggaa gattcatgca acaatccatg atcattctac
    33601 cttgttgtga aggatcacta ggatctcccc aagcttgtat aactcctgcc ttctcccaaa
    33661 aacgctgaag taatgtttct gcagcatcaa ttgaagcttc cttcattttt cgatttcccg
    33721 ttattttata atctgctacg gttgataaag aatacaaaaa accaagatcg tgatgattag
    33781 tgataatttg acagtcaatc ctgtggataa aatctatatc tgctttttca gctgtttgtt
    33841 gaaataaagg atcacccgtc ttctcaaata acaaccaatt aattcctgtc caaaatcctt
    33901 ctgtccaacc aatattgtct cttaatggat aacgtccttt tataggacac tcgcttggga
    33961 attgatactg actgttactg atacgtttga ttaacctctc cgtgtgtctg cgacaacgct
    34021 cgatctcatt attccattct ttttccatta attctttata acattcattt atccattttt
    34081 ctggatgtat tatctcttca aacatcactt tatcaagcat ttccccacca gcctttagca
    34141 taattttagt ctttttttat tgtttttatt ttggatagtt agcccggttg tcccatcaaa
    34201 atcaattttg ggacgattgt tccatacttc taaactataa tcccgatctt gatgagcaac
    34261 cactcctgtt ccgagccaat gggttccttt tccaaatgta ccctctaatc caggaataaa
    34321 agtgttttcc catgtcatca agtttgtatt gggcaaaccc ataatagctg tggattttcg
    34381 tttaatagta tcgtcactta aacagataat acctgaaaag ccagcttcac tcaccctata
    34441 gttttccttg tcttcttcta tgattattcc ttctttctca ggagaacatt caatagcaaa
    34501 ccctgtctca tatagaacat attctgtatc taattctact ttgtgtacgc gaatctgcca
    34561 gccttcaaaa ggaataagcc aagttctgat ttttgcctgt aaagtaggtt cccatgtaga
    34621 ggcaagtacc tctttcttac atacaatgtc tcgtacatta tttcgaacga tgaactgtcc
    34681 ataggttttg atgctctcat tcttaaaact ggtcgtgata tatgtgtccg ctcgctgaat
    34741 ccctagcatt gaatccattg ccatttctgc tttcaattga ttcgctcgcg gtatcgaaaa
    34801 accaaattga ctagaataag caaattttaa atatttttca ttggtcaatg tatggaaatt
    34861 tggccctaat tgtcccgcat tcaaaaaaaa tgtatgtcca ttatcatgtt gtatgagcaa
    34921 attggcttct gaaagaagtt tagttccttc acgaataggc ataggcattt ctttagccgt
    34981 ccaatactca tggtcttcag gtagcgccaa caataaaaaa atcttattca gccataatgg
    35041 agataacgta ctattatagg gttcagtcaa tgtcaattga gggtaggtat aaccaatatt
    35101 caggatacct ttttcatcaa atatttcttt ttgcatccac catcttatat tccgattgac
    35161 taaccccttg atttcaccta atttaaaagg caaaagatcg gcccacaaca tagctgacca
    35221 aaagcttgaa actgcaaaac gataaatcat gcttcgacca aaaggcacat ttgcaccatc
    35281 ttccgcaaaa aaatgaatat agtctctaga aaaatcaata gctcggttct tatatatctg
    35341 gctaatgggc ttttctggtt ctagtacgct ataaatcaaa ccataataat gaaatgcaaa
    35401 aggattataa taatcttggc gacctcttga actgtctcga taccagccat tttgaagata
    35461 acaattttct atattatcaa atgcttcttt cagtgctgat tctttaactg gataaccaaa
    35521 tttttggata attgttccga caattatctt aaaaaattgc caattaccat ctgcacattc
    35581 atattcaaaa atttgcgaga accaatgaat aatctggctt ttttctcctg tatctaattt
    35641 tttccataat aattgttcat gatgatataa cgctaataac attgcgggca tttctaccat
    35701 acgctggtct ctttcatgta tttctcccca ataatcggga tgggaaggat ctgttccagc
    35761 ttttattcct gatagaatta atttcaacca tcgttcatct aattggtcaa ttgcgggccc
    35821 cgctccccat aaaacccgag agaatccttc cattcctgca attttatttc catatcctac
    35881 cccttgatgt atataatcaa tttgtgcgcc acttgctcga taaaacggct ggagtggttg
    35941 atttatcttt ttaaataagt caaagtaatc ctgtctggtc cttaatagtg gtactggatg
    36001 tttcattgta ttttctcctc tttttactac caaatagcta attccgactg ttctaatcga
    36061 agtaagcctt caataaaaaa ataatcagaa tagatcagtg gcacattata agtcagttct
    36121 ctatggtatg cttctgctcc gcctccaatg atcccatctt catccatatt ccaatttgca
    36181 tagtgctttt ctgtcttttg gagtatattg atagcagctt catgatacat ttttgatcgt
    36241 acagaggaaa catatcctgc taattccaga agaccgcatg cagcgcatac tccagcactg
    36301 gtatcaatat tcgaatcttt tacagcaaaa tcaatgagag gaatataacc agttttttcg
    36361 attttctgaa taaaatttac tgctatcttt tctgctatag ctagatagta cggatttttt
    36421 gtgtaacgat aacttaacgc gaatccatat aaagcccatg catttcctct gctccaacaa
    36481 gaattctcat tgtatccttg cccgccaagt aaccgaatat atttccctga ttctgcatca
    36541 aattctgcga tatgcccggt tgatccatct ttcctcacta gatacttagc tgttgtatct
    36601 gcatgctgcc gagcaatttt agagaacctc ggatcaccag tttccctcga agcccaatac
    36661 agtaatggta aattcatcat actatcaata atcatccaac caggtttatg gtcattccat
    36721 gctactaaat agttccctga aatattatag cgtccagcaa gtaccgtagc tgcatgtaac
    36781 cctagttgtc gactatgttt gttttgagac tgttgataat gtgcaatagc cgatggtaag
    36841 aataaaaaac ctacatcatg atgaacgtct gaaaaatggt acagggcaga ttctaataat
    36901 tgttcttgtt gtattgctgc ctctttgaac ttttctcgtt ttgtcttgtg aaagagctgc
    36961 cataaaatac cactccaaaa accattggtc caccagtcga ttcctctgtc ctttattacg
    37021 tctgtatatt ttccgttgta agaaatatat ggaattttat tccccattct gtctaattcc
    37081 gcatctatct tttcagaagc tacattcaaa atttttcttg accattcctt cgtctgttca
    37141 ttcatggata tcccgccatt cttttaattt ttatgaatgt ggagttgctc ctagaacgaa
    37201 ctagatggtt aacattcgtt atttttttta cttatttgca gctccaatgt cattctcctt
    37261 aaatttagac tgcgcccaca cataaatcaa gaccattggg atcactgaag ttgctaaaat
    37321 tagcataatt gcgccataat taattggctc acctgtacta gaaaccgtca tatttttgat
    37381 aattaaggga accgtatatt tatctttatc tgaaagcatg actaatggaa atagatattg
    37441 attccaactt cccataaaga taagtaaacc actagtaata atcgtaggtg ataaaatcgg
    37501 tacagcgatt ttcgtaaaaa tcacccactc attggcacca tcgattctgg cagcctccat
    37561 cagtgctgta ggaaattgac tgctatattg tcgcatcata aacactgaag ttgtactggc
    37621 aatcgaaggg atgatcacac ctgcataagt attcgataat cctaaacgac tcataatgta
    37681 aaataaagga atcaatgtgg taaaaaaagg caccatccta gatagcataa ctgtattgaa
    37741 caacagttta ttccccataa aacgatactt tgctaatgca taacccgcca ttgtagaact
    37801 ggctgtagct aaaactgtac tgattacagc aataacaatc gaattccaaa taatcaaggg
    37861 ataattatat ctcgccatga tggcttgcca gttttttaat agcatatccc caaacttgaa
    37921 attgattccg ccacttaaaa tttcttgatt actttgcgtt gaagctacaa tcatatataa
    37981 atatggcatc aaaaatatta aggcaccaaa cacaataatg acgtacgtaa aaattctgct
    38041 ttgtatcttt tgtttagtca tttttatcac ctccgaattt aaattgaata gccgataata
    38101 taacagatac aaacaccatc gtccaggcaa tagcagaggc ttttccaaaa tcaaccattt
    38161 caaaacttgt tttatataaa ttgactccta cgaccaaagc tgaattttct ggtccattgg
    38221 aagtcccaaa gaatatattt gggatattga aattacccaa gccattgata gtggctaaaa
    38281 ctgtggccat taaaatgatt ggctttagca tggggatcgt taataaaaag aattgccgaa
    38341 atttcgatgc tccatctatt tcagcacttt cgtatatctc tttagaaatc ccttgaagac
    38401 cagctaaaaa taaaatagtg ttatatccta cactaagcca aatcatcgtg atgatcattg
    38461 atattcgtat ccaaaaagga tttgttaacc actgtacacc gttaattcct agcgcattca
    38521 aaaatccatt gaataaccca gtcggattga aaaaagtcat gaatacggaa gctacagcca
    38581 ccgttgatgt aatggttgga atataataga cagttctaaa aaatcctttt ccttttaact
    38641 gtgctgaatt caataaaaca gcaaagtaaa tagctaatag catcgttatt ggaattgatc
    38701 ctgccatgat aatcaaaaca ttcgtgtaag atttaataaa caatggatct cggaagatag
    38761 ctagataatt gtcaaatcca acaaattcaa atgagtagcc aattttcata aaactaaaag
    38821 caaacgagat aatgatggga atcaaaatcg tcacaataaa gaaaataaca aaaggtgtga
    38881 ccaatccata agcacttttt ttatgagacg tatctgttgc ttttttcttg ctgttcttta
    38941 ttgtcttgct tggtaacgaa gattgcatca tcgttaagtc ctcctaatat tttttaatca
    39001 ccagttaatt ttacgccgta cttatgagca aaattttctg cttcttttag atatgaattt
    39061 tcaaaatcac ctgtttgcca atagttatat gcagcggtag tcaaataaga cgtagcatct
    39121 gaataagtgg ctggtaaaac agtatttgat gtattttcat tgattttatt caatagttca
    39181 tagtattttt gtcctcctta gtagtcaaac tttttctcag ctgcttctgt ttgataggcc
    39241 aatgagttag atgtagccac gcccagctcc agtgcgttgg ctaagcattc ttcatctgtc
    39301 agcataaatg taagaaattg ttgtgccact tgtggatttt tggcatcttt agggacataa
    39361 aaacttgatc ctcctgatac tgcacctttt cctggatttt cttttgaaaa aggaatcagt
    39421 ggagccatgc gccatttgtc agatgcatca ggaaaattca aagacatatt cgtagcatac
    39481 cagccgccag caacaaacat tgcagattct tgaaatgctt tctcagcatc ttgtgcaccg
    39541 tagaaatgta caatatctga gtcgatcatt tctttgatta actttgcagc atgtttcgct
    39601 tctttagtat tcagacggat atttccttct tcatcaagca aagattgatt ctgctgggct
    39661 agcatgttga taaacaattc ttggtcccca gattctggca aggcaattcc aaaactacca
    39721 gttttttctt ttaatgcttt tgccacctgg atatattctt cccaagaact gatttcttca
    39781 aaatctacac ctatttcagc aaattttttt ggttgataat aacaaagtgc tggggtaaaa
    39841 tcactagccc aaggaataat cgtttcatta ctcgtttgtt gacgcaaaat attcaatttt
    39901 tgatcataaa actgatcgcc atatttgtca taaaatccat attcatcagc actataaaaa
    39961 gcatcttgaa atttttccag cacacctgct gctgaagtgt ctgccatgga gataatatcc
    40021 ggcatatctt cgtgtgttac caattttggg gtgatcattt gtactgttgc tgttcccgaa
    40081 gctacattca caatttttaa gtcaatattt gctccagttt tgcggttata gatttttgca
    40141 gcattttcaa catagtttgt cagtttgccg tttgcgacat atacttctaa tgtagtggct
    40201 ttttttatca ttggatctgc gatgtttgct gttacttctt cgttagtttt tccacatgca
    40261 cttaagctga ataatagggt aatgtttagc aaagctatca ccaatttgta tctttttttc
    40321 atgtctatcc cttctttctt ttcaagttct tatataaaac gctttcactt atgtatatta
    40381 acggaattag taacacaaat atttatataa aaaaatattt tacttgtcgt tttggctttt
    40441 catttttgtt ataatagtca ttaattataa atagaaagct ttttatataa aaagagaaag
    40501 gaaagtggtt cttttgttaa caaacaacca atcctccaat cttgtcaaaa aaacactaac
    40561 ctattccata gatacccatc ttcatttatc tgctgctccc tattttcgac tactaataaa
    40621 tgaaagcgct aatttatatg ctgaatttgc acataaatct atagaactag ataatccttc
    40681 ccttttattc attgataaac agcaagaagt ccaaatcttt tctactcaaa aggaaacaca
    40741 aacttttatc attatccagt tttattttga tgagcaatta ttagcacata ttacgggaca
    40801 atctttttct atagatcaag aaataacatc tatcattact tttctgaaaa atgaacagtt
    40861 caaaattgat tatctaaaac atagtaagcc agctatttcc tatgatcagg aaatcaattc
    40921 ttttttcata ctatgccaac agatgagcat tcatttgctt tccttattag ttacttattg
    40981 tctgcaaaaa attcaattgt cttccttacc aaggcattat caacaggaac ttgctttatt
    41041 gacaatacct tcaaatgaga aagtcattcc taaaaaaaag catattcttt attcagatga
    41101 attagttgat ccaatcatac gatatttaga agaacatctt tcagaaaatt ttttgcttga
    41161 acaattagcc caaatttttt ttatcagtcc agccagctta aaaaaacatt ttagaaaagt
    41221 gaatggaaaa agtgttatta gctattttaa agaactaaaa atggaaaaag caaaacgtct
    41281 cgtgctagaa aatgaattaa cctatacaga aattgcagat atattaggat ttcattcgat
    41341 ccatcatttt tcttcagctt ttaaaaaata tacagggtta tccccttcaa agtattatct
    41401 atccttaaaa tagaaaaaaa tttttaggtt ttatccacaa attatttctc tatattttct
    41461 gtctattcaa aataaacaat aattttccgg cacaattatt tgctgttttt taccataaca
    41521 tagtacgatc aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat tggcggtgta
    41581 gctggcggtc cttcatttgc cactcgtttc cgtcgattga atgaagcgca cgaaatcatt
    41641 atctatgaac gcggagaaaa tatttcttat gcaagttgtg ctttgcctta ttatttagga
    41701 ggtgtgatca ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa
    41761 aacaacatcg atgtctttac taaacacgaa gtaacagcaa tcgatccttc tactaagcga
    41821 ttaacagtta aagacctatc cacaaatgaa gaaataaaaa cagattacga taagttgatc
    41881 atctcttctg gtgctagacc agattatccg gatattcccg gagtttttga agcagaaaac
    41941 ggctttgtcc tccgtagtgt gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa
    42001 aatccacaac atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt agctgagaat
    42061 ttcaagcatc gcggcttaga cgtgacttta atcgatcaat tgccacaagt cgctttccct
    42121 tatgatccag aaattgctaa tttagtttat gacaaattgc tgaaagaagg attagccgtt
    42181 catttagaaa caagagttac tgagatccgt gataaaggtc gagaaatagt attatcagat
    42241 ggctccgtcc tttctgctga tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa
    42301 gtggtgaaag caaccggcat acaattatca gatacaggtc agatcattgt tgatgaccag
    42361 ttacaaacca atctgccgga catctatgcg attggcgata ttatcgaaac gactagtgta
    42421 gtgactggtc agccgatcca aagtatgctt tccagtgcgg ccaatcgtca aggacatatg
    42481 ctggcagata ttttaaatgg tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc
    42541 gcaaaaatct ttgatcatac agcaagttat gcgggaatga cagaacatga attaaaagca
    42601 tcaggcataa caaattataa aactgttttt atcactccct ttgaccatgc ctatttctat
    42661 ccagaagcta caagattaaa tctaaagctg atttttgatg cagatagcgg tcgtatttta
    42721 ggtggacaag catttggaga aaaaggtgtc gataaacgga tgggagaact ttctgtagca
    42781 atcaccggaa acttgacagt ctttgatttg cctgatttgg agttgcctta ctctccaccg
    42841 tattctacta cccgtgaccc gttgaatata gctggttatg tcgcaatcaa tcaaatgacg
    42901 aatatcgtag aaacgatcaa agcaagtgat atacccgaaa acgatttgaa agaagcgttc
    42961 tttttagata tacgtgaacc taataaagca ccttctggca gtatctcggc aacaaaaaat
    43021 attccaatga atgagttacg agatcgaatc aatgaaatcc caaaagataa aaaagtttac
    43081 attactttca gaagaggatt gaatacttat acttctgccc gaatcttggc aggtttgggt
    43141 atcaaagcga ttttgattga agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg
    43201 agttattttt acttgaggag taaaaatgaa aaagtatata gggttgttgg ttggtatgta
    43261 tatagaatag aagataaatg tgaagaaatt gtgcattatt tcttcacatt tattaggtta
    43321 tagtttaaga aagtttaaac attgcgcaat ttaattattt tagctgtttc ttttcataaa
    43381 aaaacatgtt gtgactctga gacaaatcat agtccatagt gatgtctcta gagtaactac
    43441 gcctaatatt acgaggcatc atattaatct atcagcacta gtacctaaac tagcatccct
    43501 ttcgaatatt ttttcttata taataaagtt tgctttcact cctctaatct caccaaatcg
    43561 actggcttgc gttgtgtgat actaggaact gattggttta gaacgacttt tctattgtaa
    43621 tcatattttt cgccatcctc gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg
    43681 tattaaataa tttaattttt ttgttcctat ttatttaa
//
LOCUS       DACDRK010000016        41111 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDRK010000016 DACDRK010000000
VERSION     DACDRK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.016"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(396..1178)
                     /locus_tag="IWJ77_000963"
     CDS             complement(396..1178)
                     /locus_tag="IWJ77_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452598.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1243..2079)
                     /locus_tag="IWJ77_000964"
     CDS             complement(1243..2079)
                     /locus_tag="IWJ77_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9452599.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2097..3506)
                     /locus_tag="IWJ77_000965"
     CDS             complement(2097..3506)
                     /locus_tag="IWJ77_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9452600.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3520..5343)
                     /locus_tag="IWJ77_000966"
     CDS             complement(3520..5343)
                     /locus_tag="IWJ77_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9452601.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6186..6512)
                     /locus_tag="IWJ77_000967"
     CDS             complement(6186..6512)
                     /locus_tag="IWJ77_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452602.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6558..6920)
                     /locus_tag="IWJ77_000968"
     CDS             complement(6558..6920)
                     /locus_tag="IWJ77_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9452603.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6957..7172)
                     /locus_tag="IWJ77_000969"
     CDS             complement(6957..7172)
                     /locus_tag="IWJ77_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9452604.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7299..7634
                     /locus_tag="IWJ77_000970"
     CDS             7299..7634
                     /locus_tag="IWJ77_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9452605.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7710..9707)
                     /locus_tag="IWJ77_000971"
     CDS             complement(7710..9707)
                     /locus_tag="IWJ77_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452606.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9805..10080)
                     /locus_tag="IWJ77_000972"
     CDS             complement(9805..10080)
                     /locus_tag="IWJ77_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9452607.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10312..11046
                     /locus_tag="IWJ77_000973"
     CDS             10312..11046
                     /locus_tag="IWJ77_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9452608.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11085..12227
                     /locus_tag="IWJ77_000974"
     CDS             11085..12227
                     /locus_tag="IWJ77_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9452609.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12322..13713)
                     /gene="sufB"
                     /locus_tag="IWJ77_000975"
     CDS             complement(12322..13713)
                     /gene="sufB"
                     /locus_tag="IWJ77_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9452610.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13718..14188)
                     /locus_tag="IWJ77_000976"
     CDS             complement(13718..14188)
                     /locus_tag="IWJ77_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9452611.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14175..15410)
                     /locus_tag="IWJ77_000977"
     CDS             complement(14175..15410)
                     /locus_tag="IWJ77_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9452612.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15407..16693)
                     /gene="sufD"
                     /locus_tag="IWJ77_000978"
     CDS             complement(15407..16693)
                     /gene="sufD"
                     /locus_tag="IWJ77_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9452613.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16706..17479)
                     /gene="sufC"
                     /locus_tag="IWJ77_000979"
     CDS             complement(16706..17479)
                     /gene="sufC"
                     /locus_tag="IWJ77_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9452614.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17676..18524)
                     /locus_tag="IWJ77_000980"
     CDS             complement(17676..18524)
                     /locus_tag="IWJ77_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9452615.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18548..19234)
                     /locus_tag="IWJ77_000981"
     CDS             complement(18548..19234)
                     /locus_tag="IWJ77_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452616.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19227..20261)
                     /locus_tag="IWJ77_000982"
     CDS             complement(19227..20261)
                     /locus_tag="IWJ77_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9452617.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20656..20994)
                     /locus_tag="IWJ77_000983"
     CDS             complement(20656..20994)
                     /locus_tag="IWJ77_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9452618.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20996..21352)
                     /locus_tag="IWJ77_000984"
     CDS             complement(20996..21352)
                     /locus_tag="IWJ77_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9452619.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21358..22545)
                     /locus_tag="IWJ77_000985"
     CDS             complement(21358..22545)
                     /locus_tag="IWJ77_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9452620.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22736..23581)
                     /locus_tag="IWJ77_000986"
     CDS             complement(22736..23581)
                     /locus_tag="IWJ77_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9452621.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23815..24780
                     /locus_tag="IWJ77_000987"
     CDS             23815..24780
                     /locus_tag="IWJ77_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9452622.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25157..26200
                     /locus_tag="IWJ77_000988"
     CDS             25157..26200
                     /locus_tag="IWJ77_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9452623.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26262..26726)
                     /locus_tag="IWJ77_000989"
     CDS             complement(26262..26726)
                     /locus_tag="IWJ77_000989"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9452624.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26972..27919
                     /locus_tag="IWJ77_000990"
     CDS             26972..27919
                     /locus_tag="IWJ77_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9452625.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28130..30202)
                     /locus_tag="IWJ77_000991"
     CDS             complement(28130..30202)
                     /locus_tag="IWJ77_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9452626.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30135..30512)
                     /locus_tag="IWJ77_000992"
     CDS             complement(30135..30512)
                     /locus_tag="IWJ77_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9452627.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30641..31201)
                     /locus_tag="IWJ77_000993"
     CDS             complement(30641..31201)
                     /locus_tag="IWJ77_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9452628.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31351..32643
                     /locus_tag="IWJ77_000994"
     CDS             31351..32643
                     /locus_tag="IWJ77_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9452629.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32703..33821)
                     /locus_tag="IWJ77_000995"
     CDS             complement(32703..33821)
                     /locus_tag="IWJ77_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9452630.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33835..35256)
                     /gene="arcD"
                     /locus_tag="IWJ77_000996"
     CDS             complement(33835..35256)
                     /gene="arcD"
                     /locus_tag="IWJ77_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9452631.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35502..36623)
                     /gene="mnmA"
                     /locus_tag="IWJ77_000997"
     CDS             complement(35502..36623)
                     /gene="mnmA"
                     /locus_tag="IWJ77_000997"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9452632.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37027..37626
                     /locus_tag="IWJ77_000998"
     CDS             37027..37626
                     /locus_tag="IWJ77_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9452633.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37764..39050)
                     /gene="efmA"
                     /locus_tag="IWJ77_000999"
     CDS             complement(37764..39050)
                     /gene="efmA"
                     /locus_tag="IWJ77_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9452634.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39321..39665)
                     /locus_tag="IWJ77_001000"
     CDS             complement(39321..39665)
                     /locus_tag="IWJ77_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9452635.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39802..40938)
                     /locus_tag="IWJ77_001001"
     CDS             complement(39802..40938)
                     /locus_tag="IWJ77_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9452636.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11819 a         8644 c         7189 g        13459 t
ORIGIN      
        1 acgcgaaagg agtctaacgt tgtttgatat aacttagact gatcgttaac ttatttttta
       61 tatttctaca tagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
      121 tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc ttaaaaattt
      181 tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg tttaatgaaa
      241 ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa
      301 tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat ataacaacgg
      361 ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt tttaaatact
      421 tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt tttcccattt
      481 tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt actgatacct
      541 gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc tgtatcagta
      601 tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat agctgtgtat
      661 gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac ttctgtctca
      721 ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc ttctgtctca
      781 ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc tttagtgtgc
      841 atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct ttcaatagag
      901 acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa aagcattttg
      961 ttgtttacat ctacataata atcctttgac tctacttgag accttcctat acggaataca
     1021 gctttactgt ctgatatctc tataccagca tccttattat taatctgcca atctccaagt
     1081 agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact ggtagaggtc
     1141 gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc cttagcacaa
     1201 ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca cccttgaaat
     1261 atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct cttcttgaac
     1321 gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct tcactgaagc
     1381 cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg caataaaaaa
     1441 ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct ttttttcggg
     1501 aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga gcatggtttc
     1561 ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt ttccagcctc
     1621 ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat agcagccgat
     1681 ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt gaaaaacaat
     1741 gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa aaaatagact
     1801 tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct tgacgcaaat
     1861 aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta gagcatcaga
     1921 aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat cgcctttctt
     1981 tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct tatctaagca
     2041 aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg ttctctctaa
     2101 gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt ttttattcgt
     2161 tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata acgcttattg
     2221 aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc aaaaacatct
     2281 acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata atcaattcga
     2341 tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata gccattttca
     2401 gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca
     2461 gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc agctgcccgg
     2521 tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga aacatctgat
     2581 ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc aataaaatcg
     2641 ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata taatccacga
     2701 tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac atctaagaaa
     2761 gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg actggaggta
     2821 gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag attcaactga
     2881 tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat ttgaaagagt
     2941 tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg atgaaggatc
     3001 atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata ctcagtaagc
     3061 aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca accgccctgc
     3121 tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc gattccctga
     3181 ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt ggttttatta
     3241 ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt taagcctaat
     3301 tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc aacttcaaaa
     3361 ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt cccatcctcc
     3421 tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa cttctctgga
     3481 aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt gcatcaatgg
     3541 ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt cactgttggt
     3601 cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga aatcggcgtt
     3661 gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt caaagggttc
     3721 cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac cttctttaga
     3781 acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct caacaggtga
     3841 ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc ccttagaaaa
     3901 gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat tttcaacaat
     3961 gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa atccaattag
     4021 ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg cgtgaacaat
     4081 attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg aaacaaaggc
     4141 atatctgact aaaccgctca agccagcata caaaccagca attgcaccac caatcatgga
     4201 agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct cagtaattcc
     4261 cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt ttttagtttt
     4321 cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca ttcctggccc
     4381 attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg tcattgccca
     4441 agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg gaattaacca
     4501 agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca gcaacgttcc
     4561 aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca gcatcggctt
     4621 agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta cataggacat
     4681 gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag ctaataccgg
     4741 aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat ttggatgaag
     4801 caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta tggctgcccc
     4861 ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat tcaaaatgat
     4921 gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca aaatggcttt
     4981 taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag aaatgacact
     5041 aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt taattgaagt
     5101 tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa tcaccacttg
     5161 gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta aggctttcgt
     5221 atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat gggttaagct
     5281 gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt ctctgtaatc
     5341 cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca
     5401 ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca ataacgttat
     5461 tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa tttaattttt
     5521 tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa ctagggtata
     5581 tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca gtaaaaccat
     5641 tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg catataacgg
     5701 atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt gagtaaagga
     5761 tggaataaac agcattccaa caatataggc aacaatagtg atacttgttt ttttggtaaa
     5821 aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatcg atagaatgat
     5881 ttgggctagt attcttaaaa attaactagc aaatgtttaa actgatttat ttctaatcta
     5941 catgtagtaa gtatttcaat tccaccaatt atcaataaaa ataccaaaca tatccataat
     6001 aattttttct ttttcatttc ttttatattg ttataacagt gaataacata aaaattcccg
     6061 acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag gaaactataa
     6121 aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg cataatacat
     6181 ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat acggtctttc
     6241 tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat agctttagcc
     6301 cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga tcgatttaag
     6361 ttgtactgtt cttgtaaaat ctgtgttacc aatggttttg atgacttctc taatccagat
     6421 gtacctgtca tttttgaaaa aataatacct ataatagcca agacaacaat acctataatg
     6481 acaaacttct tgttcatatt tactttattc aactttttat ctcctatctc caaaataatt
     6541 tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa gattgctcca
     6601 gctacccctg ctatcatcaa aatcgtaccc attttctcat ctttttctcg atataagtag
     6661 cccataataa cagcaacagc accaaataca acaggaataa atgctaacga tactaccata
     6721 gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc
     6781 ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc ggttgtctca
     6841 tctgtttctt gtagtgctcc acaaaactgg caatacttac tttcatctgt tatttctttg
     6901 ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct tattagttaa
     6961 ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact taatatctca
     7021 gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca
     7081 cattgctgtg taacattcat tttttttgct aactccgttt gagaaagtcc tttttctttt
     7141 ctgtatcggc tgagattact tacttttttc atgattctcc cctcctgttt tattatatac
     7201 aacaatatga tgtatttcaa ctcataaaat ataaaaaact tcaaatacca ttacaaatac
     7261 aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa caatatgcaa
     7321 aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa
     7381 tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag cctaacctcg
     7441 atagcttaac agctctttct agagccctag gaacccctgt aagccatttt ctagacaatg
     7501 ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc actgaagatg
     7561 cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc
     7621 tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga
     7681 aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc ttttctcttc
     7741 cttactctcc gtccatcatc tcttgttaca cttgtaaatt gatacagagg aactatccca
     7801 gctcctgata atatcaattc ttttattgtt tcatctatcc ctgccccttt cgctaaatat
     7861 attaaaaaac tactacttgc tttcacattg tatagtttct taatatcctt agaagctcgt
     7921 tcaagagtga atccttgttt aacaaagtag cctctgtttt ttattaaagc atttttcacg
     7981 ctgttatccc caacattacg taggcgttca caaacagtgt taataagttc tttctctgtc
     8041 attagatatg tcccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc
     8101 aattgttttt ccttgctgag ttctgatcgg gaattttgag gaaatactct tttagcctct
     8161 ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaatt
     8221 ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa
     8281 acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc tttctcttcc
     8341 tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt
     8401 atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt
     8461 ttaccatagt aagaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca
     8521 ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc
     8581 atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg
     8641 tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg
     8701 ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca
     8761 actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc
     8821 ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt
     8881 aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc
     8941 tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct
     9001 tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag ttcatactct
     9061 ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca
     9121 gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca
     9181 aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag
     9241 tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca
     9301 atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa
     9361 ttatgaacta aattgataga taaaaccttt tctacgctta agtatctact atcatcttta
     9421 ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt
     9481 actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata
     9541 aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta
     9601 tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt ctctaataat
     9661 ttttcttgat ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc
     9721 ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta
     9781 tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc
     9841 agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac
     9901 atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat
     9961 atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat
    10021 ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat
    10081 atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt
    10141 tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt
    10201 tcatcgacat acacataata cctcgataaa aaaggggtgt caacagcttt gtaaaattta
    10261 tttgcaacac cccttttttg aggtatacta attttaggag gtgtggtcaa tatgataaaa
    10321 cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata
    10381 agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta
    10441 attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga
    10501 ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta
    10561 gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca
    10621 tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat
    10681 ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct
    10741 aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata
    10801 gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact
    10861 agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa
    10921 gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa
    10981 caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc
    11041 atctagtggt aagtactcct tgtaaactaa gaggagatga tttaatggca aaaatatcaa
    11101 agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc
    11161 cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc
    11221 aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg
    11281 atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag
    11341 cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta
    11401 agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca
    11461 gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg
    11521 ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag
    11581 agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt
    11641 ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta
    11701 tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac
    11761 tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga
    11821 aaacaagagc ttctagacga gttatctctt tagatactga gactctttct atcctcaaca
    11881 actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag
    11941 agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt
    12001 ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac
    12061 atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat
    12121 tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac
    12181 ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa
    12241 aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta tatatcaagg
    12301 gttctagctc ctataacagg tttacccaac actgccttcc atctcataac tgatcaatcg
    12361 attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa caaagcccat
    12421 gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat agtagagttg
    12481 ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc tgttgtggat
    12541 ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg tgtcacattc
    12601 gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc gatagtttac
    12661 ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat ttggtgcatt
    12721 gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg cgatggagag
    12781 catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca tcgttgtttt
    12841 tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt ttgctcgttt
    12901 ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac ggcaatacgc
    12961 atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg aataagtagg
    13021 tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga tcagcgtacg
    13081 ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg gaacatcgac
    13141 tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg aattcagcgc
    13201 tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga ataattcagg
    13261 gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt cgaattcatc
    13321 tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg ctagataagc
    13381 tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt ctggtacgtc
    13441 ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg catcgaaatc
    13501 gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg tttctagaga
    13561 tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca tctcacggat
    13621 cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat cgtgaaaccc
    13681 gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc ttgtggtaat
    13741 gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc caggcaagtg
    13801 tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca gcatcgccta
    13861 attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct acagttaaat
    13921 gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag gcacttgccg
    13981 tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg tcttttacat
    14041 cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc ttgcttgtcg
    14101 agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc acttgacggt
    14161 ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc catgattgct
    14221 tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc gcgagcagtc
    14281 gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc tctgacggct
    14341 accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc caaattgaat
    14401 gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa tccatcaatt
    14461 gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg gatattgtcc
    14521 ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc ggcgatattt
    14581 ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta taattcgaca
    14641 aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca ttttcgttta
    14701 ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa agcaaagaaa
    14761 tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc accatctact
    14821 acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg attgaagaca
    14881 cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt aatctttttc
    14941 gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt caagacagca
    15001 cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc catgtaagag
    15061 agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc tacccaattc
    15121 aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc atggatgaac
    15181 gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc taacgtatgg
    15241 acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc ctggagtact
    15301 tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc atcattgacg
    15361 tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat ccattcaact
    15421 tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt gctgttaaga
    15481 cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc aatcctctac
    15541 tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca tgtccagcag
    15601 ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct ttatcagaaa
    15661 gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc aagatgtggc
    15721 cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc aaaatatgcc
    15781 cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt tgtctgcccg
    15841 agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa tctgagtcaa
    15901 agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca gaagcatctc
    15961 gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat tgatctacgg
    16021 ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata ttacctgatg
    16081 attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat tcactatgtt
    16141 catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca tcttgaatga
    16201 ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg aaaataccgc
    16261 tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt actgcttttt
    16321 tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac aagtcggtaa
    16381 aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc catgaacctt
    16441 gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg cctgtttcac
    16501 tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc acccgatcga
    16561 tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag gttctcatcc
    16621 aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa tcgagccagt
    16681 ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg attcctaatt
    16741 ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca gcattccctg
    16801 tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata tagttcaata
    16861 atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt tcatttactc
    16921 ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg tctaaaattg
    16981 caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt tctccgccag
    17041 aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt aaagccattt
    17101 tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca tcacgtttcg
    17161 cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca cttggatatt
    17221 gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct aaaacatttt
    17281 tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc atgatagcgg
    17341 cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt tctccggttt
    17401 tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca tgtagatttt
    17461 ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc actcatttta
    17521 gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc ttttatttat
    17581 tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga aataagccat
    17641 tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc ctttactggt
    17701 ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg gtgaagcact
    17761 tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc aataatattc
    17821 acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt caaccctgcg
    17881 ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg gtacgtagaa
    17941 gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt ttcttcaata
    18001 tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa gccagcatct
    18061 tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat gattgttgca
    18121 ccatctttta attcggaaac atctttgact tttttagagt acaagcccat tggttcgatg
    18181 tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt gttcaaataa
    18241 ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg gttcggtaat
    18301 acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa taatggtttc
    18361 acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa tgtcgtgtcg
    18421 tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc taatcctaca
    18481 gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta catcccccta
    18541 tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct tgaacgataa
    18601 atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta ccacgctggt
    18661 agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg gccatcgcag
    18721 taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg ggaatacttt
    18781 caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca tctgctgctt
    18841 ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca taaaatggtg
    18901 ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg cctacgattg
    18961 ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac cggaaaatat
    19021 tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt ttgcgtccaa
    19081 tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc attgagatta
    19141 atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg atttgtttaa
    19201 aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat tcctactcct
    19261 tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt catcaagaca
    19321 gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac aacgttgaca
    19381 tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt tctttcgcta
    19441 aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc taatagttct
    19501 gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac ttcttttttc
    19561 ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa ctccatcaca
    19621 gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa gacgatggtc
    19681 aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt tgtttgcggg
    19741 tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc caatgctcgt
    19801 gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc ttgtccgcgt
    19861 ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt gctctttggt
    19921 acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga ccataacagg
    19981 ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc tttgtttttt
    20041 aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag ctcaagtcca
    20101 ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat tccataaata
    20161 tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc gtttttccca
    20221 ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac atctttctct
    20281 tatgattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg acgaaagctt
    20341 gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct cttccagtac
    20401 ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg tagtaagttt
    20461 gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt tacccatttt
    20521 cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca aagctttctc
    20581 gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca acttttttca
    20641 agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt tgaaacttaa
    20701 gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac cgattttatc
    20761 attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg ctttttccgc
    20821 ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg gcagattggc
    20881 aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac agtattcttt
    20941 tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg ccattttata
    21001 tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg tcctctcgta
    21061 agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc gcttttgaag
    21121 ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc ccgcttgtgt
    21181 tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc gttgaggcct
    21241 tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg tctaaccacg
    21301 ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac aattttctta
    21361 cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata tcaacccgat
    21421 accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca atgggatccc
    21481 tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga acagcagcat
    21541 catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga aacacacccg
    21601 aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac cgataaagcc
    21661 gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca catagatatt
    21721 gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa gtccttgggc
    21781 cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat caatacgtgc
    21841 aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt cagtcgtaac
    21901 caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg cgatgatgcg
    21961 ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga caagcatcgt
    22021 tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga tcaagagctt
    22081 cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat tatgtttagt
    22141 tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg gctggaaagt
    22201 aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat tctgatcata
    22261 gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag gagtcagttt
    22321 ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc caactaaata
    22381 ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat catggctaag
    22441 tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta aaatcacgcc
    22501 ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc atcccttttc
    22561 ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa gtctttccat
    22621 agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat gcagtacaaa
    22681 agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca attatctacc
    22741 agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga aggatctctt
    22801 cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa taaaaatatc
    22861 gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta gacaaaatcg
    22921 agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc acgatcatct
    22981 tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa acaccagagt
    23041 ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact agctggtctt
    23101 ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata aaaccaaaat
    23161 cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt tcgatctcaa
    23221 aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa agatagacag
    23281 caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca ttgagcaatt
    23341 tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat aataagtcgg
    23401 cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg atattcaatt
    23461 cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac agtacttctg
    23521 ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat aacttaaaca
    23581 tggcaccact tccataactt tttatgataa ttactataaa aactatttga tttaaatata
    23641 caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa ctagtaactt
    23701 agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc attttacaat
    23761 gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt atatatgaag
    23821 aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt agcttccctt
    23881 ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg cggtatctct
    23941 atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca cacgaaacaa
    24001 acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc actcatggtt
    24061 atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt catttggctg
    24121 tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca tgttatcaaa
    24181 gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt tggatttggg
    24241 gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt cttttggatt
    24301 tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt tttcgtaaga
    24361 aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg ttctctttgc
    24421 ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt agcattgttc
    24481 gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt gttcaagctt
    24541 cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc aacagctata
    24601 tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt ggatttctta
    24661 aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc tcattacgta
    24721 cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa aatcgcttaa
    24781 taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga acggccaaac
    24841 aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg caagcttttg
    24901 aacaccgttt accaagaggt cgtaaaagaa gtggtcagct tcaagaaata agaaggaatt
    24961 tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgtag aagctacttc
    25021 tggaacaccg tttattcgaa acaagaggtc gtacaagact tttgtcacga cctcttgttc
    25081 ttttgtcaaa atactggttt agtgctacta tgttcgagta tgcttttagt atttattgac
    25141 gaaaggaacc gatcaaatga aatcattgtc acatccaata aaatctgtaa aggattcagc
    25201 ggtccgcgaa atcttacccg gtttactgct ttcttttgcc atcgctgcct tcagcaggct
    25261 tcttgctatt tatctgccac agttaggtgg agcgactatt gctattttat tgggtatttt
    25321 attaggaaat acttttttta aacagcctgt tctagcaatc ggcacaaaat ttgctgaaag
    25381 caaactatta gaattctctg tttttttact aggtgcaact gtcacagctc aaacgattgc
    25441 tcaaattggt gtaaaaggaa ttgtttttgt gttactgcaa atgtcagtca ctatcatcgg
    25501 tgcttacttg attggtaaaa aactgcaatt cagcgatact tcttctttga tgatggctgg
    25561 aggaaacgct gtttgtggtt cttctgctat tgcttcaatc gccccttcca tccatgcgaa
    25621 agaggaagaa aaaggacaga tcatcacact agtcaatcta ctgggtactg tgatgatgtt
    25681 gactctccct tttcttggat tacagttgtt tggtggaaat ctcattgaaa aaagtgcact
    25741 tttgggtggt acactccaat ctgttggtca agtagtcgct ggggcaagct tacttgacaa
    25801 ttctgttgtc gaattcgcca tgctatttaa gattctacgt attatgatgc tcgttgttgt
    25861 tgtctatctg tttgaaaaat tcatgcacaa gaaacaacca gctctgtttg aaacgccaac
    25921 tgatacgcag aaaaaacgaa aattaccgct tccttggtat gtattagggt ttgtgatctt
    25981 ttgtttggtc aacagcttgt tttctttacc tgtagttatt gaccaatcgg cacattttat
    26041 cagcagctgg tttgagatca ctgctttagc ggcaattggt ctgcgtttag actttgcaaa
    26101 attcatgaaa gaaggtacac gcttcttatc ttatggatta gctgtcgggc ttctccaaac
    26161 agtgatggct ttactattta tttggctgat caaagtttag aatgaacatg aaaaaacagt
    26221 tgtgattctc gcatggaaca atgtccgaat cacaactgtt tttaattttg cagatatcct
    26281 tctaacaaca acaattggcg tttgatttca accccatcta ttgcgtttcc catatatcct
    26341 gtcagttttc cattttgccc gatcactcga tgacaaggaa taacgattgg caatggattg
    26401 gttcgatttg cctgcccaat cgctcgcaca gctttcggac tatcaacagc aacggctact
    26461 tctttatacg ttcggatttc tccataagga atggtcgaca aagctttcca gacattttgc
    26521 tgaaaaggcg tacctctctt tatagataaa ggaacatcaa attcttttct ttttcccgca
    26581 aaatattctt caaactgttc ttgtgtgcgc gttaaaattc gctgattcgc tgattcgctt
    26641 tctgaaattg gctgaaaaga tacagcagtc aatccggatt catctgcatc taaccataaa
    26701 aagccgatag gtgtgtgtat tttcattttg tttgctccta gtcaatagac tggaacttcc
    26761 tcctttttct ctatccaaaa ctacttgcca actaataaaa aatgttctta aaagtttgat
    26821 aaaccgagga aacatggtaa actaaatacg ttagtttaca aaaagaatac ctgttctttt
    26881 acgtaaaatc aaattttaaa ggtgttattt aaggtttatt aaattttgaa tgtaaaccat
    26941 atataaacat acgattgaaa ggatagaaac gatgaaagta tcaattgttg tcccatgttt
    27001 taatgaagaa aaaactgttc ctcttttttt tgatgaggtt gaaaaattca aaggtgccca
    27061 tgagttcgaa tatttgttta ttgacgacgg atcaaaagac ggtacgttag cagcaatcaa
    27121 gaagctagcc tcagcttatc cttatgtccg ctttgtctca ttttccagaa attttgggaa
    27181 agaagcggct ctgtacgctg gattgcaagc ttctacagga gatttagtga ccgtgatgga
    27241 tgttgatctg caagatccgc ccgaactgct tcctgagatg atcaaccgga tcactacttc
    27301 tgatatcgat tgtgtcggca cccggcgttc gactcgtgaa ggcgagcctg cgatacgcag
    27361 tttctttgcg aaaaaattct accagttgat caataaaatc agcgatacag aaatggtcga
    27421 tggcgctcgt gatttccgct tgatgactcg tcaaatggtg gatgccgtcc tagaattaac
    27481 agaatacaac cgattttcaa aaggattatt tagctgggtc ggatttaaaa cagaatatat
    27541 ttcttatgaa aaccgcgaac gtgtggctgg cgaaacatct tggtccttct ggaaattatt
    27601 caactactcg attgatggta tcgtgaactt ctcggatgca ccgttaacga ttgcttcatt
    27661 tatcggcgct ttgtcttgtg tcggttctgg tattgcaatt ttgttcatta ttttgcgggc
    27721 attgttgttt ggtgatccta catcaggatg gccttccatg gtctcgatct tcctgttcat
    27781 tggtggaatc caattgttat gtcttggaat catcgggaaa tatattggga aaatcttctt
    27841 agaaacgaaa cagcgtcctg tttatatcat aagagaaaca gaaaaagtga agccaaatga
    27901 attgaagcct caccaataat atacgtcaaa tgaagtaaac atttttttaa atgaaaaagg
    27961 actgtgacaa aagtcttgtc acagtccttt tttccgaata aatggtgttc cagaagtagc
    28021 ttcttcggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt
    28081 cttatttctt gaagctgacc acttctatca caacctcttt catatcttat tacttcttca
    28141 ttgctgcttt tagtgctgct gctaatgggc tttcttctgg ttcttctgct tgtgcatatt
    28201 ttttcagcaa gcgtcgttct tcatgtttgg tcattttctt cttctgctct tttttatcaa
    28261 gcaatttttc tgtcgttcca tcgtatttac aacggaaata agcaccgttt ttaccttcga
    28321 tgatttccat tttccggtga cattgcgggc atcgatgatt agagactttt ggatcttttc
    28381 ttctgcggta gttgcaatct gaattggtgc agacatagat tttgccgtca cgtgtgtttt
    28441 tctcgcgcag attggcccca cattctgggc atttcttttg ggtaatcgag aaatcttgat
    28501 attcttgttt gcttgatttg atctcattca ccaagcgttt tgtttctttt tcgattccat
    28561 tcaagaaggt tcctgcttgt ttctttcctg cagcaatagc ttcaaggtct ttttcccagc
    28621 tttctgtcaa atctggtgtt actaaagagg gatttaccaa tactaataat tgcttacctt
    28681 taggagatac ttgcagtcct tgaggggtac gttccatcag ttcagatttg attagtttct
    28741 cgataatctc tgcacgcgta gctggggttc ccagactgta tttttccatt tgaccaagca
    28801 agcttgcttc tgacaatggt ttaggcggtg cagatagttc ttttttgatg gcgaaatcgg
    28861 gattgattct cattcctttt tcccatttga ctgtttccgt ctcagtttca gtttcgcctt
    28921 tccatcctgc ttggacgaca cgattttgcc gaaaaatgaa tcgttcttta ccaaattcaa
    28981 ccgtaacttt tgtttggctg actttatggg gttgagcaaa cagtcctaaa aaacgagaga
    29041 caatcatttg atagattttt cgctcttcat tacttaattt ttcataacgt gctctgtttt
    29101 cagttggcaa aagtgcatgg tgatcggtca ctttactgtc ttggaatact tttgtctgtt
    29161 ggacgatggc tccgtttttc aaatactgct tgacctcagg agcaaagtcg ctgactgctt
    29221 gcaatcgttc tttcatcgta cttttcatgt catttgtcag atatttgctg tctgtacgcg
    29281 gataagtaac gatcttatgg gtctcataca aactttgtac aagacttaat gttttctttg
    29341 cagaatagcc atagcgttga ttggcttctc tttggatctc tgttaaatca taaggcaatg
    29401 gcgcagattc cgtttttaat ttttcttgga cttctgtgac agttcctttg cttgtactga
    29461 tcaattgaac aagtttttgt gcttcttctt gtgtttttaa agcaaattga tttttttgaa
    29521 gcattttggc ttgttctttt ccagcttgca acgtgatcgt gaaatacgtt tgtggacgga
    29581 actgctcgat tttcttttct tggtctctca ctaatgctag tgtcggtgtc tgcacacggc
    29641 ctgctgataa attatccgcg tacttcacag ttaaggctct ggtcacattc aatcctacca
    29701 gccagtccgc ttttgcccgg gcaagagcag attcatacaa tgcatcatat tctttcgcag
    29761 gtcttagttg cttgaatcca gttttgattg ctttatctgt ttgagaagag atccataacc
    29821 gcttcactgg tttattgaaa tgcacatact ctaagatcca gcgagcaaca agttcccctt
    29881 ctcttccggc atctgtcgca atgactgctt ctgagacatc ttttcgctga gccagctgct
    29941 tgattgcttt taattgatgt cctgttttag gaagtggttt gattcctagt tttttaggga
    30001 tcattggcaa cgtgtccatt tgccagctct gccattcctt attcagatct tccggcattt
    30061 tcaatcctaa taaatgacca agggcccaag taacgatcac cttgggtcct tcataataat
    30121 ttttgtgctt ttgattagca ccaagcacct tgcttaggtc tttcgcaacg cttggttttt
    30181 ccgctagaat tagttgtttc ataaaattcc tttctgcttt ctactgtgat gggctgatga
    30241 tttttcaaca acgcaagtcc cttgtattgc tgtaaatcat cgtcacactc ttcacaccag
    30301 cgctcatctc cgtcattcca aaaggcgctg agaaagttac tttttgcatt gatatattca
    30361 taattttgat ggatttcgtt ccattgtttt tcgtacgctt gtcgtgcttc ttcgaaagac
    30421 tcgaatgttt cttcttcttc aatatcttct tcccagtctt caaaaaacca ccaaggctca
    30481 ttgtccccga acatggtaat tacttgatac atactcgtgc tccttctttc agcttttcta
    30541 gactcatctt agtcactttt caagaaagtt gcaagaattt tctcttataa aagggtcatt
    30601 taatgaacat cttaaaattg ttacaaatga atctgattgt ttaaggcaaa gtagcgataa
    30661 agttattgat catccaaaaa tagctgtctt ctggatcgat tgttggttct tttgtaaagt
    30721 atcctaaaaa acgtaaggaa agataaccgt ggatcatact acgaagttcc ctgcttcgat
    30781 gaactttttc ttctttcgtt aattgaaaag cctcaaatat ctgcaaaatg atctgattcg
    30841 tttcatggat tccatctaat aaaacagctt cattggtatg cggtacactg atcagtaact
    30901 catacactgc ttgatttgaa aaagcaaagg atcgataagt ttctgcatag atctgcagtg
    30961 cttcctgacc actttttccc acaagagccc gtcgcagctg gtcattaagt tcctgcagaa
    31021 gtttcgctgt tagcgctgtt ttgacatcac gaatattttt gaaatggtta tacagcgagg
    31081 gagccttgat atccaagcgt ttggctaaat gctgaaaggt tatttgctga acatccatct
    31141 cttctgctag ttctcgaaag cagtcgatga ttttctcttg agacagattt ctttttttca
    31201 tttccatgtt tcctctcata aaataaatcc ttcactcttt cagtttacca cgaaaaagaa
    31261 agagaaaaaa gagactttca ttgataaact acatattgta gtttataata aagagctgta
    31321 gatgaaaatt ttcagaaaga aggagtctaa ttggctgcaa gaataaagga aaaattattt
    31381 aggaaaagag aaacttggga acaaaacttg tttgtcttat ggtttggaac atttatggct
    31441 ggtatcggat ttagtttagt catgcctttt atgtcacttt acatagatac gttaggtaat
    31501 tatagtacgt cacaattgaa cttttggagc ggattgactt tttcatctac tttcttggtg
    31561 actacgctta tctctccttg gtggggaaaa ttagccgacc aaaaaggaag aaaattgatg
    31621 ctgttgcgtg cttcattagg aatggcaatc gtcatttctt tgatgggttg cgtgactagt
    31681 gtgtatcaat tagtcgcatt gcgtctgctt caagggattt tttctggtta tatcagtaat
    31741 gccactgccc ttgtcgcaac tgggacgcca aaagaaaaaa gtggtcaagt tcttggaacc
    31801 ttagcaactg gttctgttac aggaacatta cttggaccgc ttcttggcgg gatctctgct
    31861 tcagcgtttg gctatcgccc tacctttttc attacaggga tcatcttatt tttcgtcttc
    31921 ttgttgagtc tgtttttcgt ccatgaaaaa tttgtcccag tgaaaaaaga aaacatggtt
    31981 tctgccaaac agattttcaa agacttgaaa tatccacacg tcgtcatcgg tatgttcgtc
    32041 acaacgatga tcatccaagc atccaacaat tccattagtc caatcatcag tctgtacatt
    32101 cgccaattgc tgcatggaca tgggaatgtt acgttgatca gcggaatcat tgcttcgata
    32161 cctgggattg ccactttgat agccgctcca agatttggtc gcttaggtga caagattggg
    32221 agtgaaagaa tacttgcgat tggtttgggt tttgcgattc tggtttatat cccaatggca
    32281 ttcgtcacga atgtttggca attagctgcc ttacgttttt tgattggtat ctctgatgct
    32341 tgtctgctcc ctgctgtcca agcattgatc acaaaatatt cgcctcatca agctgctgga
    32401 cggatcttca gttataacca atctttccaa gcaactggta acgtagtagg gcctatgatc
    32461 ggctccagtg tttccagtgc atttggttat cgtggcgtct ttctttcgac ttctcttctt
    32521 gttttggtca acttcttgct tgtacgacac aatacacaag agatccatca gcaagaaact
    32581 gcagaagaaa aagcttcagg agtctccata gcttcagggc actctccttc tcatagacaa
    32641 taagcgtaaa tgaaaaacac tgcttgaaca gttgtcacag actgtccttg cagtgttttt
    32701 gtttaggcct gttttttcaa gaactcagat aagaggaata atcccttttc caaggtttct
    32761 tttttacaac agtaacctag tcgggcatgc ttcggtaaat caaaggcact tcctggtact
    32821 aatagtactc ctgtttcttc taataaattg atacaaaatg tctggtcatc ttcttcgatg
    32881 gctaatttaa tgaaagaagt agagacgtaa ttgggagcga caagttcaac tttcggttca
    32941 ttggctaccc attgttttaa aatcacaagg ttctctgtaa tgatcttttt gtttcgcgcc
    33001 aatatttttt ctttattctt caatgcatgt acagcaagat catctgacag gactccgccg
    33061 caaatcatcg tgtagtctcg atactttcga aaaacttctg ccaactcagg tccagtagct
    33121 gtccagccaa tacgaatacc tggaatggag taggttttgg atagggaatt ggttgctata
    33181 cctttttcat agacatctac gatcgacaag aattcacctt tatccgttaa aggcgcatat
    33241 acttcatcta tcaatacata tgcatctact gtacgagcaa tttctgcgat tttttctaaa
    33301 gttttccgat ctagcaatgt gcctgtagga ttgtttgcac tattcaaaca gatgattttt
    33361 gtttcaggtt tgactaaggc ttctagctgt tctaaatcaa actgccaatc ttcttcttcc
    33421 ttcaaatgaa caaagtcaac agcggcacct aaagatttgg ggatgtcata taattgctga
    33481 taagtaggaa gcatcgaaac aacatggtct cctgcttcca ctaacgcgta tagagctaaa
    33541 agattagcac cagtcgctcc attcgtttgt aagatgtttt ccggatcgac ggtttgataa
    33601 agagaagcga cttcttgttt gaaaacatct gatccttcga tccagccata gtccaatggt
    33661 tcgtttttgc gtttttcgaa aaatttactt acgctagtcc catctaagcc aacgagttcg
    33721 tctaatgtca atgcttcgat cgagctttgt gcaatatcat ataccgcttt cttttcccat
    33781 ttatttagcc attcttctac accgaaattt gcgattttca tgtttctttc ccccttatat
    33841 cttgatccag cctacagcaa gcaacccgat tgaaagcagt gcggccacac aaatcagccc
    33901 aatcattact ttttcttttc ccgtaatttg atgtttggtt tcacgacaag cgatccagta
    33961 gaagcaaaaa ccaggaagat aggcaatact acataaaagg acataagaaa atcctgctaa
    34021 aatgatgccc atcaattcaa aaactacagc aatcccaccg atcactactt gttttttgat
    34081 tccttctttt aaagaaaact ggacttgata gatcccgaca agtagataac aaatcaaaat
    34141 tgccgctgta cataaagaat aggcaaattg ataggcataa tcagtaacta agaaagtcaa
    34201 taagaataga ttagtcaaga ttgcagtaac aactaacgaa taagttgggg catttttttt
    34261 attcgttttt ccccaacctt tcggcaacag accgtctcga gccatcaaca tcgttgtttc
    34321 tgctgggagc atcgtccaag acaaccaaga accaatgatc gaaataatca agccgatatt
    34381 gatcagcgct gctccccaat ttcccacgat tccttttaaa atatttgcca tagcaggttg
    34441 tgaaatagtt gctaactctt cttgagataa gacgccgtat ggcaagatcg aagcaaggat
    34501 atagataaac aataaacaaa gcagaccaat gatcgatgct tgctgggctt ctgaacgttt
    34561 cttcgcgcgg ttggctaaaa cacttgcgcc ttcgatccct acaaagaccc acatcataac
    34621 catcaaacaa cctttaatct gcaaccagat cgttccttgc tgccctgttc cattcactag
    34681 attatcagat acgttacccc aaaaatcagc agtgaatata ttcactttaa atgcgataaa
    34741 tacaaatatt aaaaatagga ataatggcac taatttacag atcgtcacga ttgtattgat
    34801 gacgctcgct ccttcaactc cattgtttac taataaagtc agaccccaaa taaaaatact
    34861 tgcaaataaa atagaaggaa cattttgtcc acctttgaat atcgggaaaa attctcccat
    34921 ggcgctcatc agcatcgttg caaaagcaac atttcccagc cacgcagaca accagtaacc
    34981 ccaaccactg atgaagccac ctaatttacc aaatgaagct tccgcgtaac cgaaaatacc
    35041 accatctaga tctggacgct tttctcctaa atgattcagc gataatacca aagctaaaat
    35101 accaatccca acgattatcc atgaaaggat cgctggccct ggagctgcag aactagccag
    35161 atcacttgtg atcccaaaca caccgcttcc gattgacgaa ctgacaacca gagcaaccag
    35221 tccccatttg ctaatacccg tcgatttctt ttccatttcc atcaccctca cttataaaat
    35281 tgaacaaatc acttgcaaaa gattcccttc tattttgcaa ctgctcttgc ttaacaaaaa
    35341 gcaactttat tcatattatt ggatattatg aactttttac acaagtattt caagtataaa
    35401 atgaataaaa attaatttta ttgcaaaaaa acagctatgc tatccaaaag aaaaggtgtt
    35461 tcttttgtta gccatagctg ttttgattca tttaatatcg cttaaacgta ttgtaaaact
    35521 tttgtttctt gatatgcgtg gtcgatcaaa ccaccaccca gacattccat tccatcataa
    35581 aatacaactg cttgtccagg agtgattgca cgtacaggtt cgtcgaatac gacttctgca
    35641 cgattatcat ccaataagcg aactgtcaca ggaacgtctt gctgacgata acggaatttc
    35701 gctgtacact tgaattcttt tggcattggt tcattcgtcg taaagtggat ttcacttgca
    35761 tccaagcttg tggcatacaa tgcaggatga tggaaccctt gccccacata taatgtgttt
    35821 gtcgctagat cttttcctac aacaaaccag ggttcttgcg aatctcctcc gccaccgatc
    35881 cctagtcctt gacgttgacc aatcgtataa tacatcaagc cagcatgttg tcctttgact
    35941 tcaccatcaa gagtgaccat atttcctttt tttgctggca gatagttgct caaaaattgt
    36001 ttgaagttct tttcaccaat aaagcagatt cccgttgaat cttttttctt agcagtcgca
    36061 aggcctgcgc gttcagcaat tgcccgtact tctgattttt ccatgccgcc tagagggaac
    36121 atcgtttttg aaagctgttc ttgtgataat tggctaagga agtaagtttg atctttatta
    36181 ttgtctactc cacgaagcat gtggaccacg ccgttttcat cgcgagtgac ttgcgcataa
    36241 tgtccagtag ctacataatc ggcgccaaga tccattgcat aatccaagaa tgctttgaat
    36301 ttgatttctt tgttgcacat cacgtctggg ttaggtgtac gtcctgcgcg gtattcggct
    36361 aggaaatatt caaatacacg atcccaatat tctttttcaa aattcacaga gtaataagga
    36421 atcccgattt gagcagctac tttcgctaca tccttataat cttccgtggc cgtgcataca
    36481 ccgttttcgt ccgtgtcatc ccaatttttc atgaatattc cgatcacgtc gtatccttgt
    36541 tcctttaaca aaagtgctgt tacagatgag tcgacaccgc cgctcatccc tacgacaacg
    36601 cgtgttttgc tgttgtctgt catgatatca ccatcatttc aattagattc tgaaacatct
    36661 acgagttgaa ggtcgcaact tttcagtatt ttacattata cgtcatattt ctgaaaattt
    36721 catcccttaa ttatcaagtc gtaaaaatac tgttctactg gacctattca gtacacaaac
    36781 atgatagtca accaaaaaat cactcatctt ttgagcattc gatataaaaa attggcatac
    36841 tcataaaaag ggccagcctt tcggcaataa ggaataattt tataaattgt gaaaaactat
    36901 ttttcaaaat tgcttcttat cgcttaaagc tgcacgtctc atttacaacc ttttcttttt
    36961 agatgttatg agttgctcag ataagtgatt ttgtttactc taagaaaaaa gggaggcaaa
    37021 agatacatga attactttat cagtgatatg cactttttcc atgaacgctt attaggaaaa
    37081 aacgactttg ctccgcggct ctttgcaaat gtggatgaaa tgaaccaaca gttgatcact
    37141 agctggaatg cagtaataaa agaaaccgac catgtttatc atctaggcga ccttgctatg
    37201 catccgcaat atgaaaaagc aaatgcagaa attttggaac tggtcactca actgcaagga
    37261 acaatccact tgatcaaagg gaaccatgac agtcgcgctt ttttccgtta tttagaaaaa
    37321 aatgatccta ggcttactga cggaagtccc aaattttatt tttatgatgt aggtgccatc
    37381 gttaaattcg accatcaaca atattacttg actcattatc cgatgttgct cgggcctaac
    37441 gacactatcc gcaatcttca tggacatatc catcattaca gcgtacctat cgagaacgat
    37501 gtcaatgtag gcgtagacgc accggaaaga gaactcttaa aggaaaaact accatttggc
    37561 actccgttat ctgagcaaca aatcaaagaa atctatgaag caaagaggaa gtacgatagg
    37621 tcgtgaaaaa actgccctat atcaagtatt aaacaaataa agataattac tcatattttt
    37681 catttgagta ggcttattgc cacaggaaca ttaggatcag aaatggagtt gtaacaagac
    37741 ttctgtcaca actccattcc atactattcg tctttttgct catggtgttc ttttaccagt
    37801 tttttctgta attctttatc ataatttcgg gcagctgacg ttaaaaataa aacgattccg
    37861 cataataaaa tcccgattcc ggaaaacaag aacatttttt ctacccctaa acgatcagct
    37921 aatggtcctg caaataataa tccaattgga cctgtcaggt tcatcaaaga gttgaagttc
    37981 cctaagacac gacctaaaac attcgattca taactctgct ggatcattgc catcagcagt
    38041 gtattgaaat atggcgttgc acaacctgct cctgcattca agataagaaa gtacaagaac
    38101 ccttgactat cgtttggaac caatccgctt gcaccgatcg tcaggcccat aatgatgtag
    38161 gctataataa taggtttcat tcggtctttc catgttccta taaaactgat cagtgcaccg
    38221 cctaagagca ttccgactgc gtagactact tccactgctc ccgcctgtcc aacggaacca
    38281 ccaaagtaat ctagcgtcat caatggatac agactgattg ctggcatgaa caacagcatc
    38341 acaaatgcac cattgatcgt tatgtaccat aaccctttat tatccatcaa ttgctgcagc
    38401 ccaaattttg catctttaaa aatagtaagc ggaacttcca atatttcagg agaaactttt
    38461 gggatcttca caaaaatcaa taagccgaca ccaaaaactg cccccaatac atctaataaa
    38521 ataaggtaat tgacaggaac gactgaaaac aaagccgcac ctagagctgg cgcaatgatg
    38581 aaattcgctg attggaccat tcctaactgt ccattcgtcc ttgttatgtg agaagatgga
    38641 acgattgtcg gcataatcga ctggatcgtt ggcatttgga atgtttgtgc gaccgaacgc
    38701 ataaacaaag acacaaaaac tagccaaatc gggaaggatt ctgaaatcgt tccgactact
    38761 gctaaaataa gcgcgaaaat cgctacaata atatccgtga caatcaaaag agctttttta
    38821 tcccagcgat ccactagcgg tcctacaaat gggcttagta aaaccatggg aatcattcct
    38881 agtaaagtag caaaactcaa aattgtcgcc gaaccggttt ctcttgtcaa ataccagatg
    38941 atcgcatatt ggacgaccat actagtgatt cctgacaaaa actgtcccga taaaaagaac
    39001 aaataatttc tcttccaatt cgttaaaacg accgactgtt cattttccat aaacatccct
    39061 cttttctttt tcattttgta tagcgtttgc acaacaatca ttatacggga ttcttttttc
    39121 aaaaaatacc tttttcccta aataacaatc gtttcaattt aaatttcttt acttagaaac
    39181 aataaatcag caaaatatta aaaataacaa catgtgttaa atttaaaaat aaaaaagaca
    39241 actaatttgc gtcgtcctta caaagttagt catctaacaa taggccgttt caaaactagt
    39301 tgtcttttta tatccacaga tcattcgact ttttcgaata acccacggct aatcatccca
    39361 tccatcagaa aataaaattt atcttggctc attttctgtt ttggatcttt gaaaatattg
    39421 actgctttca atccatttgc tgtagtgatc gacattttca atgtttgaat atcttttgat
    39481 acagtgattt ttaatttgaa tccttctttg ctttgcggcg tggcatctaa tggtcggatc
    39541 aattgataat tgcctccgtt tgtttctgta aatccattat cgcgcaatgt atatcttttt
    39601 gcatcaggat gcaaacgata gctaattggt gcgcccagta ccgttgctgt tgtttcaaat
    39661 gccatgattt tcacctcgat aaaattagtt tctctttatt ataaaagttt atcctgattt
    39721 ttactaggtt tttcagttaa gttccctaaa aaatgaaggc gttctctcag acatctgttc
    39781 atataagaat gacttcacta gttatttttt gacagcacga actaattgat caaggaaata
    39841 ctggatgtcg tcttcggtat ttcccaaacc aaagctgata cggatggatt cttttagtat
    39901 cggtgtatct tctccataca ttgcttccaa tacgtgtgaa ggctcgatat ttcctgctgt
    39961 acaagctgaa ccagtagaaa tagcgacccc attaagatca agcttcatca gtagcaaatc
    40021 atttgcttcg ccaattaagc gaagattcaa cacatgaggg agtttattcg ttaaatcacc
    40081 atttacttga taagggattt cgtttgcttc taatgtatca agaatcaatt gctggaatcc
    40141 tttatatttg ttgttacgtg cttcacgttc ttcaggtgac agcagttcaa ctgcttttgc
    40201 cataccccag atccctgcta aattttctgt acctgcacgc cgcttttctt cctgctcccc
    40261 gcctaaaagc aatgcaggga tatttactcc gtctcgttta tataagaaac cgacgccttt
    40321 tggcccattg attttatgag cagagatact taataaatcg attcctaatt cttctggatg
    40381 gatcgtttgg tttccgtacg cttgtaccgc atctgtatgg aaataagctg gatgatccgc
    40441 taaaagttca ccgatttctt tgattggcat caaatttccg atttcattgt tcccgtacat
    40501 cacagagacc aagatcgtat cttctcgtaa cgcttttttg acctcttcga tcgacagatt
    40561 ccctttttca tctacaggca gatatgtaac gtcaaacccc tttgtctcta aatatttcat
    40621 ggtattaaga acagcaggat gttcgatagc tgtagtgatt agatgtctgc cttctttttg
    40681 acgcgagaac gctgtttcta agatagctgt attatcccct tctgttcccc cactgttaaa
    40741 gatgatctcg tgaggttttg cttgcagact gtctgcaatg atttgtcttg ctgtttccaa
    40801 tttttcatgc gcgtggcgac caaatccatg tatgcttgaa ggatttccaa acgtgtcttg
    40861 catgattgcc gtcatcgctt caattacttg tggatgcatg ggggtagttg ccgcatgatc
    40921 cagataaata gttttcactt cattcatccc ttccaaatta ttgctaggca cattctagca
    40981 taaaatagcg aacaaaccaa agaagagctc atttttgtct agaggacgtt tcatgggcat
    41041 agaaaaaagg agctgtgaca aaagtcttgt cgcagctcct tgaagctgct cacttctgtc
    41101 acaacttctt t
//
LOCUS       DACDRK010000017        37214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDRK010000017 DACDRK010000000
VERSION     DACDRK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.017"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..808
                     /locus_tag="IWJ77_001002"
     CDS             59..808
                     /locus_tag="IWJ77_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9452637.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="IWJ77_001003"
     CDS             complement(1232..1402)
                     /locus_tag="IWJ77_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9452638.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="IWJ77_001004"
     CDS             complement(1596..1775)
                     /locus_tag="IWJ77_001004"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452639.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="IWJ77_001005"
     CDS             2162..3274
                     /locus_tag="IWJ77_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9452640.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="IWJ77_001006"
     CDS             complement(3352..3645)
                     /locus_tag="IWJ77_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9452641.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="IWJ77_001007"
     CDS             3813..4127
                     /locus_tag="IWJ77_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9452642.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="IWJ77_001008"
     CDS             4148..6127
                     /locus_tag="IWJ77_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9452643.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="IWJ77_001009"
     CDS             6140..6466
                     /locus_tag="IWJ77_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9452644.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="IWJ77_001010"
     CDS             6482..7000
                     /locus_tag="IWJ77_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452645.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="IWJ77_001011"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="IWJ77_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9452646.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="IWJ77_001012"
     CDS             complement(8436..9941)
                     /locus_tag="IWJ77_001012"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9452647.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="IWJ77_001013"
     CDS             complement(10033..10623)
                     /locus_tag="IWJ77_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9452648.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="IWJ77_001014"
     CDS             10802..12448
                     /locus_tag="IWJ77_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9452649.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="IWJ77_001015"
     CDS             12497..13216
                     /locus_tag="IWJ77_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9452650.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="IWJ77_001016"
     CDS             13695..14642
                     /locus_tag="IWJ77_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9452651.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="IWJ77_001017"
     CDS             14654..15574
                     /locus_tag="IWJ77_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9452652.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="IWJ77_001018"
     CDS             15615..17072
                     /locus_tag="IWJ77_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9452653.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="IWJ77_001019"
     CDS             17417..18031
                     /locus_tag="IWJ77_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9452654.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="IWJ77_001020"
     CDS             18024..19748
                     /locus_tag="IWJ77_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9452655.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="IWJ77_001021"
     CDS             19745..21196
                     /locus_tag="IWJ77_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9452656.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="IWJ77_001022"
     CDS             complement(21669..23822)
                     /locus_tag="IWJ77_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9452657.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="IWJ77_001023"
     CDS             complement(23824..24870)
                     /locus_tag="IWJ77_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9452658.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="IWJ77_001024"
     CDS             25020..26309
                     /locus_tag="IWJ77_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9452659.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="IWJ77_001025"
     CDS             26322..29024
                     /locus_tag="IWJ77_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9452660.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29009..29866
                     /locus_tag="IWJ77_001026"
     CDS             29009..29866
                     /locus_tag="IWJ77_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9452661.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29937..30794)
                     /locus_tag="IWJ77_001027"
     CDS             complement(29937..30794)
                     /locus_tag="IWJ77_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9452662.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31005..33176
                     /locus_tag="IWJ77_001028"
     CDS             31005..33176
                     /locus_tag="IWJ77_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9452663.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33244..33585
                     /locus_tag="IWJ77_001029"
     CDS             33244..33585
                     /locus_tag="IWJ77_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9452664.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33861..35615)
                     /locus_tag="IWJ77_001030"
     CDS             complement(33861..35615)
                     /locus_tag="IWJ77_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452665.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35626..36462)
                     /locus_tag="IWJ77_001031"
     CDS             complement(35626..36462)
                     /locus_tag="IWJ77_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9452666.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36464..37147)
                     /locus_tag="IWJ77_001032"
     CDS             complement(36464..37147)
                     /locus_tag="IWJ77_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9452667.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12005 a         6542 c         7633 g        11034 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaggg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctctgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgagtcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggtttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atgcttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggcc ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aattttagaa aaattaagta tccctaccac
    29101 aacaccagaa gaaacattgg atcaggtatt tgaatttttt gatcgttatg aattgaaggg
    29161 aatcgggatt ggctcattcg gcccgattgg tatcgacaaa acaaaagata attacggata
    29221 cgttttggca actcccaaaa aagggtgggc acaattcgat tttcttggca caatcaagga
    29281 acgctacaat attcctgctg cttggacaac ggatgtcaat gctgctgcat atggggaatt
    29341 gaagcaagga gcagcacaag gaaaaaatag ttgtatttac cttaccgtgg gaacaggaat
    29401 cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg atcgcacatc ctgaaatggg
    29461 acatatttgg gtgaaacgcc atccagatga ccattatgaa ggtacttgtc cttatcataa
    29521 agattgttta gaaggattag cagcaggacc gtctattgag gcacgcacag ggatcaaagg
    29581 gcaagatctg ccgcaagatc atcctgtttg ggctattcag gcttattata ttgctcaagc
    29641 actgatcaac tatacgttga cgttagcacc tgaaaagatt attttaggcg gtggggtaat
    29701 gaatcaggat catctccttc aaaaaatccg tcagcaattt gttgaattga tgggtggtta
    29761 tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg ggactgccga atgaaagtgg
    29821 gattatcggt agtttgctgt tagcagaaca cgaacatcaa aagtaagcag acaaattaca
    29881 caaaaagaac agagtcttat cattatctgc tgataagact ctgttctttt ttatttctat
    29941 tcccataatc caggtaaagc tttaccgact attttttctt gagtaaatgg atggatcatg
    30001 tgcagttcta atgcatggag catcagtcgt tcagctgttc tattttggta gagagggtct
    30061 ccaacgatcg gatgtccctt atgagctaaa tgaactctga tctggtgtgt acgtccggtt
    30121 tccaaaacac aatagacttg tgtttgttca gaaagtactt tgttgacagt gacatgtgtc
    30181 aaggcgctct gaccattttt atcatctacg accctttttc tccgatcatg gcgatctcgt
    30241 ccaattttat tggtgatggt catatctttg gcaatttctc cccatacgat ggcttgatat
    30301 ctccgataga tcattttttg ttccaacatt cttccaagta tcggcaagac aaatggattt
    30361 tttgcaaata aaacggcacc gcttgtttct ttgtccaagc ggtggacaac gtaaggtcgt
    30421 tgctgtttag tgtcgagata agcagctaaa tgattgagta aggtactgtt ttctgtcggt
    30481 tcatttggat gagtcttcat tcctgcaggc ttatccacta taatcaaatg ctcatcttca
    30541 tataaaacac ggatgtgctt cgcatctcct aattgaactt cttgataatt ataatctgtt
    30601 tcttcgaata acaatgtgat ctggtcattg ccctttactt catgatggaa caaagcaggt
    30661 tgttgattga tccaaacgtt tttacgtata cgcaaaaagt gtcgaacctt tctaggaact
    30721 aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag gatggttcat aggtaaagtg
    30781 atcgtgattt tcacaagtgt actcctttca gtgcaattgc agactaattg taccattttt
    30841 aaaacatatt taagaaaaaa cttacatttt tctgttcatt cttatgttac tctacttgca
    30901 ctaacgtaaa aaaacatatt gaactacaac ttaagcagga agaaaacact ctttcactat
    30961 ggtaaaataa gacgattgaa tcaaatagac aggagaagaa gcaaatggat tttcaaagca
    31021 ttttagaaaa gataaaagct gctttgatca gtttttggcg ttggatcaga ccatatctcg
    31081 gacaattcca tcgatggcga aagcgcatct ggaaaaaata tcatgtgaac aaattgattt
    31141 tgctgattgg tttgatctgt gtcttagtga caagtatcta cctctttatt ttagcaaaac
    31201 aagcaaatgt cgaaacgtta aaatcaggat taagccaatc gacagtcatt tatgataaga
    31261 aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac atatgtagaa ttaaacgcga
    31321 tttctcccta tatccaagat gcagtaatct cgacagaaga taggaatttt tacgagcatc
    31381 acgggtttga tatcaaaggg attgcccgtg cagcagtccg aatggtcttg tctggcggaa
    31441 catcaggggg cggaggaagt acgatcactc agcagcttgc caaaaatgcg tatctgacat
    31501 tagaccaaac gtttgatcga aaagcgaagg aattattttt ggcaatcgag atcgaaaaga
    31561 agtacagcaa agaagaaatc ttgaccatgt atcttaacaa tgcctatttt ggcaacgggg
    31621 tatggggtgt ccaagacgct tccagaaagt attttggagt agatgcaacg aatgttacgc
    31681 tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc tggtatttat aatccgatcg
    31741 atcacccaaa aaatgcgaat gatcggaaaa acacagtact aagtgtaatg gaagaaaacg
    31801 gaaaaatcac aaaagaacaa gaacaagcag aaagccagac agatatcagc gcgtatctta
    31861 atgacacata cgataattca gattctggtt atcgttatcc ttattatttt gatgcagtga
    31921 tcgatgaagc agttaacgag tatggtcttg atgaagaaga tgtcatgaat aaaggctata
    31981 aaatctatac agctttagat caggattatc aaaagcaatt agaagctact tatcaaaaca
    32041 gtgcattatt cccagcaaat gcttcagatg gtgccatggt ccaatctgct tcagtagctt
    32101 tagatccgaa tacaggtggt gtacaagcac tagtaggacg aagaggagat catgtcttta
    32161 gaggattcag ctttgctaca cagatgcgtc gttcaccagg atcaacgatc aaaccattaa
    32221 gcgtatatac accagcactg gaagctggat acaagccttc cagtatcttg aaagacgaac
    32281 cgcaaagtta ttacgatgcg cataattttg acgggacata ccaaggagaa gtaccgatgt
    32341 atcaagctgt tgcacaaagt ttgaatttgc ctgctgtatg gcttttgcat gagatcggtt
    32401 tacaaaaagg atatgataaa gcagaagaat tcggcctgcc tttagcaaaa tctgataagt
    32461 attatggctt agcccttggt ggactggaaa aaggagtatc tccattgatc atggcgggtg
    32521 cttacagtgc atttgccaat gatggtcaga tgtatacgcc gcatcttatc acgaaaatcg
    32581 ttgattcgac tggagcggtg atcgtcgata acacaaacaa aaaaccaaaa caggtcatat
    32641 caaaggaaac agccgatgaa atgaccagca tgcttcttgg tacattttca aatggaacag
    32701 cggctgctgc gaatcctgca ggttatacga tagcagggaa aacaggaacc acagaaacta
    32761 atttcgatgc cacaaaagca aatgaccaat ggatgatcgg ttacacgcca gacgtagtga
    32821 tttcgacatg gatgggttac gaagaatcca gcaagctgca ctatttggaa gggacaagtg
    32881 gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg catccttccg tatacttcaa
    32941 aaacaggatt tagtgtagca gatgcctatg ccacaggagg acaagtggta gctgcagatc
    33001 aagttggaaa cacaggaaat tctaatggag agtctggaaa ttggcaagat aacttgaatc
    33061 aatatggtga acaagcaaaa gaagggctga aaaacttcgg cgatatggtg aaagaaggcg
    33121 ttcagggaat tggtgaagcg gctaaagacc tctggcggaa atatcaagga gagtagcccc
    33181 tttgaaaacg aataacccca tgttacaata gagagtaact actaaaagaa aagagggcat
    33241 aacatgtcaa acatctacga tagcgcgaat caaatcgaac gtgagatccg tgaattacca
    33301 gaattcaaag cgttgaaaga agcgtacgag aaagtacaag caaacgaaga agcacacaaa
    33361 ctgttcaaag acttccaagc gatgcaaatc gaattacaag aaaaacaaat gagtggacaa
    33421 gagttcagcg acgaagatgc aatgaaagca caagaaatgg ctgtgaaaat ccaatcagaa
    33481 gaagtaatca atgacttgat gcaaaaagag caaggcttca gtacaatcat caatgacttg
    33541 aaccgtatca tcatgacacc agttcgcgac ctttacagcg aataaaacta ttttatgaaa
    33601 caacagaggg tgtgaaagag acgttcggcc ttgagcgatg acaaaattgc ttcttattgc
    33661 cgataggctg tctcttgaac accatttacc aagaggtcgt gagcctaccg tttaactctg
    33721 agttacaagg aacaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg
    33781 accgaggagt tggcaggaga acaccgttta ttcagaaata ggagctacga caaaagtctt
    33841 gtcatagctc ctattttttg tcattcattt ttcaagcggt agaaatagcc tttttgagct
    33901 aacagttttt cgtaagtacc tatttcaacg atttttcctt catcgatcac aacgatttta
    33961 tctgcctctt tgatcgttga aagccgatga gccacaataa aagtagtggg tgtttctgtc
    34021 aggtaattca gggcttgctg gatttttttc tctgactggt tatctaaagc tgaggttgct
    34081 tcatctagca aaatgatttt tggctgacgt atcaacgctc gtgcaatgga gatacattgc
    34141 ttttggccac cagacagctt gttgccatgt tcgcctacta aggtatccag accatcagga
    34201 agctggtcaa tcagtgaaga aagttgcgca gcttcgatga cttcatctaa acgttctttt
    34261 gatacgtttt tcaagccata agtgatgttc tctttgatcg aagcggagaa caacaaagtg
    34321 gtttgaggaa caacggctaa ataatttcgc acacttcgca tatccagttc atccaaaggc
    34381 tgatcatcta gaaaaatttt tcctgaagtc ggctggataa atccaatcaa taagttaagg
    34441 agtgtagatt ttccagagcc agatggccca acaaaagcaa ttgtttctcc agctttcacc
    34501 tctagagaaa aatgatcgat aatcggtttg agtgagtccg gataaagaaa agaaacatcc
    34561 tcaaaacgaa aatttccatc caaatgttgg atttttttct taccgtgata agtttctaca
    34621 tcgccagcaa tcaacagttc gccaatagaa gtgatagatt ccatgccttt tgaaatcgta
    34681 ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg tcgtgaaata cgtctgatac
    34741 atcacgacat cgccgggttg gatgcgccct tgtaaagcta atatcccagt gaaaatcaaa
    34801 caaatcagct gaaatatctg aaaactagcc cagctgactg atccgaaaac agattgcagg
    34861 atatctaagt ggaatccttt ttcatagatt tgagtaaaat gcttatttaa gcgagttgtt
    34921 tcgtgttttt ctaacccatg agctcgagca atcggtgcta attcgatcac ttcgatcact
    34981 tttgcagaag tctcttccat ttcttgcctg aagtctgagt tggactgtct gatcctttta
    35041 cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca ataagaaaaa catgaaaaca
    35101 acaggacttt tataaagcgt gatacctaaa gctacgaata aattgacagc gatatttaat
    35161 ccgctgacaa atacttgaga ggacaacgtt tggatagctt ctacatcacg gatgatcttt
    35221 gactggattc gtccagattg caattctttt tgataaggaa tagatagttc ttggattttc
    35281 ttgataaggg tcgctcttag tccagcttct acttgacgga tcgaagtgct atggtatttt
    35341 acatgaaaat aattcgtcaa tatattttga ccaatcagac aagccatcaa aaaagcgttg
    35401 aaataaatgg cctgcaactg agaagaatct ttcgatgaag ctgcattgat tacattggca
    35461 atagcgatcg gcattaacca agatggaaga tgtttgataa taaaaaagag tcctgacatg
    35521 gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg tctgaaaagc atttgcatga
    35581 tatttttgat aacttttctg tattcgttcc tccataatct tctccttaac aatcttggta
    35641 aataaactgc aagtgttcta ttgcttcagg aacataaatc tcaggagtag cttcaagagt
    35701 ggcgtacatt agaggacgct catatttgat ataatgcagt accgggcgga aatctaaaca
    35761 gcccttgcca acaggtacca tgcggatctt tccattttcc atgatatagt ctttgagatg
    35821 aagcgctgta atataaccat gtaaattgtc caaggcacct cggatcactt tttcctgatc
    35881 agcatgattt tctttatgca tcaaattcgc acaatctaaa atgattttta aatttggtga
    35941 agctacttca tcgattaaac gtttagctaa ctgatagtta tagattggat gattgatccc
    36001 tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga gcgactaatt caatgataga
    36061 atttctggcg atctgataag cttcttcagt aaaattttct tttgtgtaac cgacagagac
    36121 actaccggtt tccgtagcaa tcatcttggc ttgataggca gagcacagag caatttgctg
    36181 tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt ttgctggata ggttgatata
    36241 gcatcctaaa atggaaagtt cgatattgtt tttttggaag tattcaccaa aataggcagc
    36301 actcccaagt gtcatgtcag ttaatttaag ttgttcagga aatgcttttt gtggtgcaaa
    36361 ttggatatgg gacaagtgaa gctgtgatac tttttgaacg atttcttcac gatttcgaac
    36421 atctaaatcg tgggcacgga tgcctaaatt taatggaaac atctcaaact cctttttgta
    36481 aataattagc aagaggcaga cgggattctt ttatcaattc aacaattatt cgggcaactt
    36541 tatcagcgcc ttctggacta aagtgggtat tgtcttttag tccttctggg taatttttac
    36601 aagtatcagg cataagatgc aggtagaatt tttttgtctc ttcttttgag aaggtctgga
    36661 acaattcttg tgatcttgag aacacatcta aaagaggaat atggtgtttc tcagcaaatg
    36721 cacgcatcgc ttttggatag tctccaagta tatctggatt caatgttccg ttttctaagt
    36781 aatctcgtcg cgtcacagat gtcagtaaga ccatttggac gttcgctttt tttgcgcatt
    36841 caaaaaatat ctgcagattt tttaaatagc taccgaaggg aagcgtacct ctttcttctt
    36901 ggattttctg gtcgttatgt ccaaattgta caaataagaa atcattcggc tgaaactgtg
    36961 aagctagacg ggctaacctt ccttcctgga taaatgattt ggtgctgcgt ccattctttg
    37021 cttggttatt gatctggatc tcgggtgaaa aataagcaga gattttttct ccccaacctg
    37081 tctcgggacg cttgttttca tctttttctg ccgcagtaga atcaccggca atatggatag
    37141 taggcatgtt atctcttctt tcataaaggt ttataggcaa tgaagcagaa cagcaagaga
    37201 agaagacgga gcaa
//
LOCUS       DACDRK010000018        35652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDRK010000018 DACDRK010000000
VERSION     DACDRK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.018"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            332..2098
                     /locus_tag="IWJ77_001033"
     CDS             332..2098
                     /locus_tag="IWJ77_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9452668.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2200..3459
                     /locus_tag="IWJ77_001034"
     CDS             2200..3459
                     /locus_tag="IWJ77_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9452669.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3606..4907
                     /locus_tag="IWJ77_001035"
     CDS             3606..4907
                     /locus_tag="IWJ77_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9452670.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4910..5761
                     /locus_tag="IWJ77_001036"
     CDS             4910..5761
                     /locus_tag="IWJ77_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9452671.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5774..6589
                     /locus_tag="IWJ77_001037"
     CDS             5774..6589
                     /locus_tag="IWJ77_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9452672.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6740..7906
                     /locus_tag="IWJ77_001038"
     CDS             6740..7906
                     /locus_tag="IWJ77_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9452673.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8136..8783
                     /locus_tag="IWJ77_001039"
     CDS             8136..8783
                     /locus_tag="IWJ77_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9452674.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8867..9811
                     /locus_tag="IWJ77_001040"
     CDS             8867..9811
                     /locus_tag="IWJ77_001040"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9452675.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9946..11397
                     /locus_tag="IWJ77_001041"
     CDS             9946..11397
                     /locus_tag="IWJ77_001041"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9452676.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11418..12254
                     /locus_tag="IWJ77_001042"
     CDS             11418..12254
                     /locus_tag="IWJ77_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9452677.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12254..12913
                     /locus_tag="IWJ77_001043"
     CDS             12254..12913
                     /locus_tag="IWJ77_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9452678.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            13039..13728
                     /locus_tag="IWJ77_001044"
     CDS             13039..13728
                     /locus_tag="IWJ77_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452679.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13734..14690
                     /locus_tag="IWJ77_001045"
     CDS             13734..14690
                     /locus_tag="IWJ77_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9452680.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14734..15246
                     /locus_tag="IWJ77_001046"
     CDS             14734..15246
                     /locus_tag="IWJ77_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9452681.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15502..16050)
                     /locus_tag="IWJ77_001047"
     CDS             complement(15502..16050)
                     /locus_tag="IWJ77_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9452682.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16139..17320)
                     /locus_tag="IWJ77_001048"
     CDS             complement(16139..17320)
                     /locus_tag="IWJ77_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9452683.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17556..19064)
                     /locus_tag="IWJ77_001049"
     CDS             complement(17556..19064)
                     /locus_tag="IWJ77_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9452684.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19223..19414
                     /locus_tag="IWJ77_001050"
     CDS             19223..19414
                     /locus_tag="IWJ77_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452685.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19617..20534
                     /locus_tag="IWJ77_001051"
     CDS             19617..20534
                     /locus_tag="IWJ77_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9452686.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20527..21600
                     /locus_tag="IWJ77_001052"
     CDS             20527..21600
                     /locus_tag="IWJ77_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452687.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21748..22266
                     /locus_tag="IWJ77_001053"
     CDS             21748..22266
                     /locus_tag="IWJ77_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452688.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22247..22639
                     /locus_tag="IWJ77_001054"
     CDS             22247..22639
                     /locus_tag="IWJ77_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9452689.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22715..23650
                     /locus_tag="IWJ77_001055"
     CDS             22715..23650
                     /locus_tag="IWJ77_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9452690.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23783..24562
                     /locus_tag="IWJ77_001056"
     CDS             23783..24562
                     /locus_tag="IWJ77_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9452691.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24555..24872
                     /locus_tag="IWJ77_001057"
     CDS             24555..24872
                     /locus_tag="IWJ77_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9452692.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25116..26726
                     /locus_tag="IWJ77_001058"
     CDS             25116..26726
                     /locus_tag="IWJ77_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9452693.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26883..27731
                     /locus_tag="IWJ77_001059"
     CDS             26883..27731
                     /locus_tag="IWJ77_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9452694.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            27962..28441
                     /locus_tag="IWJ77_001060"
     CDS             27962..28441
                     /locus_tag="IWJ77_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452695.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            28474..30702
                     /locus_tag="IWJ77_001061"
     CDS             28474..30702
                     /locus_tag="IWJ77_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9452696.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            30831..32474
                     /locus_tag="IWJ77_001062"
     CDS             30831..32474
                     /locus_tag="IWJ77_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9452697.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(32547..33092)
                     /locus_tag="IWJ77_001063"
     CDS             complement(32547..33092)
                     /locus_tag="IWJ77_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9452698.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33115..33600)
                     /locus_tag="IWJ77_001064"
     CDS             complement(33115..33600)
                     /locus_tag="IWJ77_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9452699.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(33590..34402)
                     /locus_tag="IWJ77_001065"
     CDS             complement(33590..34402)
                     /locus_tag="IWJ77_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9452700.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(34541..>35652)
                     /locus_tag="IWJ77_001066"
     CDS             complement(34541..>35652)
                     /locus_tag="IWJ77_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9452701.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        11671 a         6411 c         7118 g        10452 t
ORIGIN      
        1 ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta tgtttcattc
       61 ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag aaaattggag
      121 aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag actaaaactt
      181 ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa
      241 actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac ttatagtgat
      301 gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt atcatcgacc
      361 ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc ttcgtactag
      421 gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca taacaactga
      481 aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta
      541 ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc atgttgttgg
      601 ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc gagaaaatgt
      661 gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg atcgattcac
      721 tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa
      781 cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag atccagaccg
      841 agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt acttgacgga
      901 ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca atcataaata
      961 cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa
     1021 cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg tattcaatca
     1081 tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa aatcgatcta
     1141 taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga ctgacttacc
     1201 agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca tgccgaaact
     1261 aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt attggataaa
     1321 agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc atcatttttg
     1381 gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc ttggagagat
     1441 ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga tgaattatgc
     1501 atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt cacaaatggt
     1561 cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag gaacattcaa
     1621 cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta acaaagaatt
     1681 gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta tctattacgg
     1741 gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta tgatatggga
     1801 gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt ctctaaggaa
     1861 acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa atgatcgtga
     1921 agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt actttaatca
     1981 gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc aaaattgtca
     2041 gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca tgtgttgaaa
     2101 actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc attataataa
     2161 gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag aagtgtgaaa
     2221 cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc ttgtggcaat
     2281 ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat
     2341 tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg
     2401 aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc gttagatggg
     2461 cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg tgcgttagga
     2521 cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga tgaaacagac
     2581 aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat cgagaccctt
     2641 gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa agatttggag
     2701 aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa tacaggattt
     2761 ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta cggtggctat
     2821 gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc aggtgctgta
     2881 gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg aatgcaagac
     2941 ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac tgcagcaatc
     3001 attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta tggtgtagcc
     3061 aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg aaaaggatgg
     3121 gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga ttatgtaacg
     3181 aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc aaaccaacaa
     3241 gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat tgaacaatat
     3301 gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac aggtgcagaa
     3361 aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc agatgaagca
     3421 gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata accgtccatt
     3481 tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat ggaaaattat
     3541 cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt agagggggca
     3601 ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac
     3661 ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc gtttttttcg
     3721 ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca ttggtaggat
     3781 tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg attgaaggca
     3841 ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat cttttagacg
     3901 cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag
     3961 tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc gcttatcttc
     4021 tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca tttactaatt
     4081 atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct aaaaactttt
     4141 ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt tcgtggaccg
     4201 tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc acagcggttg
     4261 ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc ttattaccat
     4321 gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac gatagcgtag
     4381 gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta gatatcagtg
     4441 caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta gtgcagggct
     4501 ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg atttcagaag
     4561 acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt tcaaacatca
     4621 cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat acggggaatt
     4681 tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc gtaggcggag
     4741 gagctggttc tactgatatt cttatttcct ggatctataa acttacgact ggaacttctc
     4801 cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt
     4861 cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt
     4921 tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact tttttctttt
     4981 aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg ccttcaagtc
     5041 aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag gaaactttac
     5101 tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga tcatttctgt
     5161 cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt atagtcgcta
     5221 tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga tggtaccgac
     5281 tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc tcgataagta
     5341 ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt ggctgatgaa
     5401 aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg atggggcagg
     5461 gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc tcgccgttca
     5521 agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt tcctgcttcg
     5581 aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa
     5641 ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga tttgtctatt
     5701 attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa caaaaggata
     5761 ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac gtacgccaac
     5821 tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga tatttgtatt
     5881 tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga tccaagcgat
     5941 tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt
     6001 ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga tagctgtgac
     6061 ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc atggaacttt
     6121 tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg gtatacgccg
     6181 ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc ccgaagattt
     6241 ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca ttttatctta
     6301 tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat tcggggcttc
     6361 ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat tcaaagagag
     6421 tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag ttgtagggtt
     6481 tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg tcttgctgct
     6541 tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac aagtttaagc
     6601 gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct
     6661 gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta atagggtagt
     6721 ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc
     6781 tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac
     6841 ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac
     6901 aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg
     6961 aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa
     7021 agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga
     7081 tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag atccaacttt
     7141 gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa
     7201 taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat
     7261 cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac
     7321 tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca
     7381 tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt
     7441 tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta
     7501 tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag
     7561 ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta
     7621 tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga
     7681 tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca
     7741 tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct
     7801 atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt
     7861 gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta
     7921 ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt
     7981 tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata
     8041 aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa
     8101 tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta
     8161 gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg
     8221 ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag
     8281 caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata
     8341 tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa
     8401 ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg
     8461 atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga
     8521 atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg
     8581 atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa
     8641 gcggtacggc aacagattgg gatttggata aagatttagg tactacttat tgggaagtca
     8701 aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac
     8761 tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta
     8821 gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac
     8881 gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat
     8941 gactacccag gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag
     9001 ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg
     9061 gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct
     9121 tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat
     9181 gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat
     9241 cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt
     9301 tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat
     9361 caaattgcct ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca
     9421 gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac
     9481 agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta
     9541 tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat
     9601 ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg
     9661 tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg aaattttttc
     9721 tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga
     9781 atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc
     9841 gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt
     9901 tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca
     9961 gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata
    10021 cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc
    10081 gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc
    10141 ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct
    10201 cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg
    10261 ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca
    10321 gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga
    10381 tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt
    10441 gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct
    10501 tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt
    10561 gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt
    10621 atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa
    10681 aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag
    10741 gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca
    10801 gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc
    10861 gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa
    10921 gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct
    10981 acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat
    11041 caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa
    11101 gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta
    11161 gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca
    11221 cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa
    11281 aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa
    11341 atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag
    11401 aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt
    11461 ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg
    11521 tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt
    11581 ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac
    11641 ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt
    11701 caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa
    11761 aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag
    11821 atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca
    11881 atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg
    11941 ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg
    12001 attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg
    12061 agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc
    12121 tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa
    12181 tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt
    12241 cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga cttcacttcc
    12301 tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg
    12361 aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt
    12421 atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct
    12481 gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac
    12541 gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc
    12601 aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat
    12661 cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct
    12721 agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa
    12781 gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg cactgattta
    12841 ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatgca
    12901 tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt
    12961 ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata
    13021 tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac
    13081 caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa
    13141 ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc
    13201 tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga
    13261 cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct
    13321 acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct
    13381 ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct
    13441 ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag
    13501 cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag acgttcagaa
    13561 aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt
    13621 caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg
    13681 aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact
    13741 atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag
    13801 gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta
    13861 aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc caaaatcccc
    13921 aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata
    13981 gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac
    14041 ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac
    14101 agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc
    14161 tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt
    14221 atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat
    14281 atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta
    14341 gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac
    14401 attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag
    14461 gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa
    14521 tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa
    14581 agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt
    14641 ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg
    14701 taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa
    14761 gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc
    14821 tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa
    14881 agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc
    14941 tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct
    15001 tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa
    15061 ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc
    15121 gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg attcacttat
    15181 caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc
    15241 aattgactat aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat
    15301 catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt
    15361 ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc
    15421 tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct cggcttatcc
    15481 tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc
    15541 ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa
    15601 ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc
    15661 acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac
    15721 taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca
    15781 atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc
    15841 atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga
    15901 aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt
    15961 aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga
    16021 aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt
    16081 gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatatt
    16141 ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa
    16201 ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac
    16261 cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg
    16321 ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt
    16381 catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga
    16441 ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc
    16501 cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca
    16561 tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga
    16621 aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat
    16681 agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga
    16741 tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag
    16801 acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt
    16861 ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt
    16921 ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc ataaataatg
    16981 gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca attggcatca
    17041 atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa
    17101 caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg
    17161 ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca
    17221 caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta
    17281 atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa
    17341 ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta
    17401 cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg
    17461 tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa
    17521 tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa
    17581 gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt
    17641 ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa
    17701 gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg
    17761 atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca
    17821 agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca
    17881 tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact
    17941 tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga
    18001 atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg
    18061 acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg
    18121 aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca
    18181 acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga
    18241 taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca
    18301 acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc
    18361 atattgacat cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc
    18421 aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt
    18481 tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc tgggccagga
    18541 gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct
    18601 tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc
    18661 ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg tccactatag
    18721 cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc
    18781 aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct
    18841 ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt
    18901 ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactagcttc
    18961 ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac
    19021 tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa
    19081 gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca
    19141 aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag
    19201 tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca
    19261 ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca
    19321 attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat
    19381 tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc
    19441 gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg
    19501 gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat
    19561 ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga
    19621 aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg aatgcggatc
    19681 aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc
    19741 tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc
    19801 ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg
    19861 aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat
    19921 ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca
    19981 cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt
    20041 tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca
    20101 aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag
    20161 atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct
    20221 tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat
    20281 ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa
    20341 aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag
    20401 aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg
    20461 aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt
    20521 ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat
    20581 catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa
    20641 gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac tccgacgaat
    20701 acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa
    20761 ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta
    20821 gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca
    20881 tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc
    20941 gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat
    21001 tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa
    21061 aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg
    21121 atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg
    21181 ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata
    21241 ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat
    21301 cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg
    21361 acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg
    21421 gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata
    21481 acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct
    21541 gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga
    21601 ttggataagt tggattacca gatgtcattc agataattct gttccaaagg acttgcggca
    21661 gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt
    21721 gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta
    21781 ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca
    21841 atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc
    21901 atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc
    21961 caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca
    22021 gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga
    22081 ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac
    22141 cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt
    22201 tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat
    22261 ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta
    22321 tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac
    22381 gttacaagaa aatggtcaaa acttttcgga aaaagaagtg ttcttcaaaa aaggcgatga
    22441 tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc
    22501 gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg
    22561 gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt
    22621 caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt
    22681 cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat
    22741 gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg
    22801 ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg
    22861 cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt
    22921 gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct
    22981 tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc
    23041 ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga
    23101 aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc
    23161 cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga
    23221 cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac
    23281 cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc
    23341 cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag
    23401 cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat
    23461 aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg
    23521 taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc
    23581 tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc
    23641 tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt
    23701 ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt
    23761 tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt
    23821 gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt gcggagtatt
    23881 gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc
    23941 cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac
    24001 attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg
    24061 ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga
    24121 aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc
    24181 tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt
    24241 tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt
    24301 cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc
    24361 aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc
    24421 aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc
    24481 taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca
    24541 ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg
    24601 tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct
    24661 ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag
    24721 atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga
    24781 ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac
    24841 ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat
    24901 tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga
    24961 tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt
    25021 tattttagga attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat
    25081 gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa
    25141 cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc
    25201 cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg
    25261 ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga
    25321 ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt
    25381 ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt
    25441 tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag
    25501 ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat
    25561 atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg
    25621 tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca
    25681 tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta
    25741 tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt
    25801 ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac
    25861 taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga
    25921 tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat
    25981 tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa
    26041 cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa
    26101 tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga
    26161 ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa
    26221 gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc
    26281 tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag
    26341 caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga
    26401 tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt
    26461 gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc
    26521 gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat
    26581 tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg
    26641 gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta
    26701 aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa
    26761 cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta
    26821 atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac
    26881 acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc
    26941 atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag
    27001 gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta
    27061 ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag
    27121 ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat
    27181 tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca
    27241 ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt
    27301 cccttgatga aatacttgca catatcgatc gaatccgaga taatacctac gcttatctga
    27361 ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga
    27421 ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat
    27481 tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa
    27541 gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag
    27601 tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg
    27661 gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt
    27721 cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tattccaaca tcatcttgga
    27781 ctttctacat gttactaatg agcgtgtaaa agaacgagat ggtgttttta tttctatcaa
    27841 tgaagctctt tttctttgca atcctcactt caatcctcta taatgtcgca tatagtttag
    27901 taaatgagta aactactaat ttacttattt actaaaaata aaaggatgag gagttaaaat
    27961 aatggaaata ccaacaaatt taaaacataa accagtaatc gttgtagaag actatgataa
    28021 agttgatgga agaaatgcat taaatacgga tgcaaaaggg ctttcactgg gccttgctca
    28081 gtggaatgat cgtggaaaag tcgatatctc aggcaaggta tggcgacata ctggagaaaa
    28141 atggtcacga cagtcggaag aattaccaat cacacgtatt ttagatttag cgatcttagc
    28201 tgcacaagga agtttatact tccaagatgc ttatcgttat gaaaaatttt atgatccaga
    28261 aaatccagtc gtagacatca ttggattaca agggaaccga atgacggtag aagtagatac
    28321 caaaaatcca aagatcgatg aagacatcat tttgtattac gatacactac aaaaagacgg
    28381 agaactgatt ggggaacgat tccgtatttt aaaacgtctt ttagatgagc taggatatta
    28441 atagacagag atgatcaaaa ggggaagaga agcatggaga atttctcact aacagaaaac
    28501 cgaacctttt cacggctaga aaagcaaatg atctggaaga aaccgaaaag ccatcggaca
    28561 agtgcagaag aatggcggat cgcctccgaa atcaaaggga attggcaaga tcctgaaatg
    28621 aaaatcagta acgtactttt ggaaggagat gccgggtcgg ggaagacaca attagccaaa
    28681 gcactatctg ctgatctgca acttccctat acaaaagtga cctgttttgc agatatggac
    28741 aaatcagatg ttttcggagc cttattacct gtagtagatt ctaataagga acaagaccaa
    28801 gaattactag aagctattta ccaaacagat acacttgaag ctgtactgac actgatccag
    28861 aaccattacg gcattgatcc tttttctgct aaagaaaaat tagcagagct agttcaaagg
    28921 atcgaagctg attatccaga tgcaatccat tacagctatt acccttctga aatcgttcga
    28981 gcgattgaaa aaggatattt attagagatt caagagccga ctgtcatccg agatgcctct
    29041 gttcttgtgg cacttaactc agcgctcgaa ccaaatggga tgttgaattt accgacagga
    29101 atcattcgtc gccatccaga ttgcgtcatt gtgatcacta caaatcgaaa ttaccaagga
    29161 aatcgcccat taaatgaatc tttaagagat cggatgcaac atgcggaaaa aatggatctt
    29221 cctgccttag aagtcatggc agaacgtgcg atggcgaaaa ctatgatcca aaaacaagaa
    29281 cttctcttaa agatggcaga gattatccgt ttgttggatg aaactgcaaa agctaacgcc
    29341 atcaaaggtg tagcaggaat gcgttcttat ttttattggg caaataccta taagcaagga
    29401 caagatttac tccagtcgat ttatccaaaa gttttgtata aattatccac tgattcagat
    29461 gaattggcaa ttcttgaaca agctttgtct gacagtggat tgctactgga acttcaggac
    29521 atattgagaa aagaaaaatg gggtcatcct caatctgagc atacaaaagg aagaaccatt
    29581 agcgccgaag aagcaaatga acgaaacatc actgaaaatg ctgaagaaat tctgcgctta
    29641 gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg gagaagagaa acaagaagaa
    29701 acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag aacgcagtag cgatatcgaa
    29761 ggaaaaaatc aaaaagaaca acaaacagga gatgattctc aagaaatgtc tgcttctgac
    29821 atggaagcac atgataagga aatcaagaag caactgaata aagaagcgcg acaaatcatg
    29881 aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc gaccagcttt ccaaggaaaa
    29941 acgccggaag cacgtgagct acacatgcaa gtaatgccaa tagtagaatc acttagccgg
    30001 caaattttag agttactgga taatgaacaa acagagacgt accaaaaagg aaaatacgaa
    30061 ggtcagcggt tcaatgcaag caaagtagca tatggtgatt tgagaaattt tgacaaaaag
    30121 aacccgccac atgagcaacc ttctttagcg gtagctgtac ggatcgatga atctgggtca
    30181 atgatacggg atgatcggat cgaagcggca aaaaaagcag cgattgccat tgccgaattt
    30241 tctaagaaag tcgatatccc attgttgatt tacggagata cagcagaccg ttcgtcaaga
    30301 gaaaagactt ctttgttttc ttataaagaa tttgaagacg gatttcaatg gttaaatgaa
    30361 aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg gtgctgcact tcgtttagca
    30421 gcagaaaaat taaacagaca atccgcaaca accaaactgt tgcttaatat tagtgacggt
    30481 cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa aagaagatat ccagtctgtt
    30541 ttgaaagaat atgaacgtca aggaatccta tttgtctccg cagctattgg ccaagacaaa
    30601 gaagaaatca agtcgatcta cggtgaaagt cgttttgtag atattactga cttgaatgaa
    30661 tttcccaagc aaatgattca actgattgcc cgctatctat gataaaataa agctcaacga
    30721 acgcacttag cagattcgtt gagcttttcg tttctagtta ccggtaacaa ggatacttct
    30781 aaaatagaat cggttataat caagagggac cgttaaagga gtgaaacgat atgatacaag
    30841 aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta tcctgctagt ttcaaagata
    30901 gcaatggcga tggtcaagga gacatcaatg ggatccgtga aaaacttaat tatttgaaag
    30961 atcttggtat cggctttatc tggatcaatc cgatctatca atctccattt gtcgataatg
    31021 gttatgacgt aagtgactat caagatatag aagagagatt cggaacgatg gaagattttg
    31081 atttattgct aaaagaagca catgaattag ggatcaaagt gattatggat cttgtcatca
    31141 accatagttc ggatcagcat caatggtttg aagaatcgaa aaaatctaaa gaaagcccgt
    31201 atcgcgatta ttacatttgg gtcgacgggg tagatggcaa agaaccgaat aattggacat
    31261 cgattttcgg cggttctgct tgggagtatt ctcatgaaac tggacagtat tatctgcatg
    31321 tttttgcaaa agaacaacca gacttaaatt gggaaagcga gaaattgaaa gaagaactat
    31381 tcaatatgat ccgatggtgg ttggataaag ggatagatgg atttcgttta gatgcgattt
    31441 ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga gaaccctttc tcaccctttc
    31501 aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa acatgttttt gaagagtacg
    31561 atatcatgac tgtcggagaa gcaagtggtg tcactgcaga agaaggacca caatgggtag
    31621 ggaaagacgg ttatttcgat atgatattcg aatttgatca tatccatatc tggcagcaag
    31681 aaaaagaagg gcaattagat gtccttaaat tgaaacttgc attaagcgct tggcaaactt
    31741 cattggatgg tatcggatgg aacgctttat atatggaaaa ccatgatgtt cctcgtgccg
    31801 tttcagtgtt tggagatact cgtcccgatt tttgggctat gtctgcaaaa gcaatcgcta
    31861 tgatgtattt ctttttgcaa ggtacgccat ttatttatca aggacaggaa atcggaatga
    31921 cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt agattcgaaa agactttata
    31981 aaaggctgct tgcggaaggt aagacaagag aagaagcatt ggatatcatt cgtgaaacaa
    32041 cgagagacaa cagcaggaca cccatgcaat ggacttcgga acaatatgct ggtttttcta
    32101 cacatgaacc ttggctgatc actaatccaa ataccaaaac gatcaatgta gaacaacaag
    32161 aatatgaacc agagtcggtc ctgcaattct ataagaacat gatccgcatt agacaaacca
    32221 ataaaggtct gatatacggt tcttataaag aatatcttca tgaacatcca caactttatg
    32281 tctatgaaag atatttagaa gatgaacaat atcttattat ggtaaactta acagaatcac
    32341 ttgctgacta tgaacttcca aaagaagcag atcagtcctg gacattactc ttgtcaaatt
    32401 cctctagcgg agaatttgaa gctaaaggca tactagcacc ttatgaagct cggttatata
    32461 aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt ggactttgcg aggaagtttt
    32521 tcagattgct ctgtcaatta ctcatttcaa taattcgctc taggaccacc gatatattta
    32581 ggacgagagc gaagggctgt cacgtgagct tgtccaaggg ttttcaaacg aggtctgaca
    32641 ggtacttgga catgtttgat atgcataccg attgatgtgt caccgatatc gataccggct
    32701 tgtccaacta cgtgttccac ttcgacagga tcattgaact ggtcaaaagc agcaacagaa
    32761 caagcacctc cagcatgtag tgcaggaaca acagagacaa tttcgaaatt ttttgcttct
    32821 gccactgttc gttctactac taatgctcgg ttcaaatgtt cacaaccttg gacagctagc
    32881 gcaatttctt taggatccaa caactctttt aacgtacgga tgatccattg accaacttcc
    32941 gcactggaat tttttccaat atgtcctcca acgacttcgc ttgtgctgca tcctaaaaca
    33001 aaaagatcac cttttttcaa gtgtgcctca gccaatatgt cattaactat ttcagtcaat
    33061 tggtctttta attctttttc tgacaacttc attttctaaa accttctttt tcaattaata
    33121 ttgcttccgt gcttgaatac gttgaagact tgcagttata ggaaaggcaa gtcccattcc
    33181 aatcagattt tggaagatgt ttcctggtat agaagccact ccagccggcc aatcatataa
    33241 aatccaaccg gcgataccat agccgatgat cataactaag cttcctaata aaagccctaa
    33301 tccccacata cgagtagaag atttgctgaa agaaccagca acatatcctt gtaacccgtg
    33361 gatcactaat gaaaaaactg cccactgcgg atatcctgaa atcaaatcga tcaatagacc
    33421 actggaagca ccaacaacta atccacccgt tggaccataa agaaaagcag cggtatagat
    33481 cccggcctcg cataatgtca caaatccatt cgtagccggt acagggatca aaattgttaa
    33541 tgaaagggca actgtcaacg caatcagcat agcatagatc gttaattgct tagttttcat
    33601 ttctcattcc tcctttttga ataccgccag accagacact tccggtatga tcgttcatca
    33661 gcacgccatt agaaatacaa tgggcaacaa attttttact ttctgctact gctttggtaa
    33721 aatctgcccc taacacaaga ttcgctgtta ttgcagaaga aaaggcacaa ccggccccgt
    33781 ttactgttgt agtgtcgatt tttttatgag aaaatacctc ataatgatca attgaataga
    33841 gaagatccac agcttcatct ccagccatcc ttgtgccgcc tttgatcaca atgttttttg
    33901 ttcctgaagc agtgagttct ttagcgagat ggatcatttc tgttaacgaa gagggaagag
    33961 cttgaccaga aagtaaagca gcttctgcca aattaggcgt aatcatcgta acaagtggaa
    34021 aaagttcttc gatgagctgc gaaatataca attgattgat cacagcttca gtttctttga
    34081 atgccaaaac agggtctaaa atgattggga tattttgttt tgtttttatg aagtctcgaa
    34141 caaccatgat tgcttcactt gaattcaata acccaatttt gataccatct aatgaagtca
    34201 ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa agatatagat tccagcataa
    34261 atcgctcatt aatatctagt gcccctatag aagtcaaggc gcttagtccg aatagaccaa
    34321 aattctcaaa agtttttaaa tctgtttgga ttcctccacc agcaaatgga tcagaaccgc
    34381 caattgtcaa gattcttttc ataacatttt cctccatgaa taagagtata acgaaatttt
    34441 caaaaaagaa aaacagccaa ttggcatatt tttatctcat ccaatttaaa aaaacaaaaa
    34501 gaaaagaaag accaaaatgg atctttcttt ccttaaatag ttaggacttt tgtgatttta
    34561 attctttctc gatttgaagc cgttgcactt tcattgtagc tgttcgcggg attgcatcaa
    34621 aatcacggat gatagggacg ttcaagcgag gaagttcatg gacttgctcc caccatcgat
    34681 tccaatccat ttctttatca ggagctaaag caataatcgg ttgtggagaa tgatttttat
    34741 cccttacaat cactacttct gccaaaaaat ctaacgaatc taataaaaag tcttcaatag
    34801 ctaatgtact attgatcgtt tcgatcaaat ccacttgccg atctttcaaa aataggtggc
    34861 catgcttatc cataaaaccg taatctccgc tatcccacca atcgccataa acattttcct
    34921 ggaaccgtgc atcttccttg taataagtca aagcccgtcc tttagacagt aattgtatat
    34981 gaccatctgt gttttcaggt aggacattcc caactgaatc tgtaatccga gcgcttgtca
    35041 tgtcttccag tccaacgccc atatctcttg catcacttgt tttcaaactc tctagtgtat
    35101 gagccttaag aatcattggt ccgcattcac tttggccata tacttgaaga aataccgcat
    35161 cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg gttgtttata gcatcaaaag
    35221 tggagtgata gtatcggata cccgaaaaag cttctggatg ctccttagca gtaaaacgcc
    35281 actgaacaaa gttattcgga tgggtctcta gtgcaattgg ccggtgagct tccaacatat
    35341 gaacaacttt tgaactttgt gcgtttgcta aaggcatcat aggaaatccc atggacatca
    35401 aagaagagat accaatattg aatctggaat gcactggaga gatgtgaaaa gcgattaatt
    35461 tcttttccga aatttttgtg aaaactgttt tttgccattt cgtccgccat cccatagaat
    35521 gagcagaatg acaaataagt ttagggatac cagttgtacc agaagtatgg gtcatataag
    35581 aaatttcatt tttgtctaaa aatgattgag aaacagacaa agcaggttgt tccattaaat
    35641 aaagaatagg aa
//
LOCUS       DACDRK010000019        34450 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDRK010000019 DACDRK010000000
VERSION     DACDRK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.019"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(29..1507)
                     /locus_tag="IWJ77_001067"
     CDS             complement(29..1507)
                     /locus_tag="IWJ77_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9452702.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1805..2740)
                     /gene="murB"
                     /locus_tag="IWJ77_001068"
     CDS             complement(1805..2740)
                     /gene="murB"
                     /locus_tag="IWJ77_001068"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9452703.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2813..3733)
                     /locus_tag="IWJ77_001069"
     CDS             complement(2813..3733)
                     /locus_tag="IWJ77_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9452704.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3846..4598
                     /gene="xth"
                     /locus_tag="IWJ77_001070"
     CDS             3846..4598
                     /gene="xth"
                     /locus_tag="IWJ77_001070"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9452705.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5462..5995
                     /locus_tag="IWJ77_001071"
     CDS             5462..5995
                     /locus_tag="IWJ77_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9452706.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6485..7009)
                     /locus_tag="IWJ77_001072"
     CDS             complement(6485..7009)
                     /locus_tag="IWJ77_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9452707.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7002..7475)
                     /gene="tsaE"
                     /locus_tag="IWJ77_001073"
     CDS             complement(7002..7475)
                     /gene="tsaE"
                     /locus_tag="IWJ77_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9452708.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7598..8578)
                     /gene="pta"
                     /locus_tag="IWJ77_001074"
     CDS             complement(7598..8578)
                     /gene="pta"
                     /locus_tag="IWJ77_001074"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9452709.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8731..9411)
                     /locus_tag="IWJ77_001075"
     CDS             complement(8731..9411)
                     /locus_tag="IWJ77_001075"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9452710.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9537..10406
                     /locus_tag="IWJ77_001076"
     CDS             9537..10406
                     /locus_tag="IWJ77_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9452711.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10432..10947
                     /locus_tag="IWJ77_001077"
     CDS             10432..10947
                     /locus_tag="IWJ77_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452712.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(10994..11557)
                     /locus_tag="IWJ77_001078"
     CDS             complement(10994..11557)
                     /locus_tag="IWJ77_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9452713.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11612..11791)
                     /locus_tag="IWJ77_001079"
     CDS             complement(11612..11791)
                     /locus_tag="IWJ77_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452714.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11868..12377)
                     /locus_tag="IWJ77_001080"
     CDS             complement(11868..12377)
                     /locus_tag="IWJ77_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452715.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12473..12853)
                     /locus_tag="IWJ77_001081"
     CDS             complement(12473..12853)
                     /locus_tag="IWJ77_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9452716.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13183..13932
                     /locus_tag="IWJ77_001082"
     CDS             13183..13932
                     /locus_tag="IWJ77_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9452717.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14116..15393)
                     /locus_tag="IWJ77_001083"
     CDS             complement(14116..15393)
                     /locus_tag="IWJ77_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452718.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15472..16116)
                     /gene="pcp"
                     /locus_tag="IWJ77_001084"
     CDS             complement(15472..16116)
                     /gene="pcp"
                     /locus_tag="IWJ77_001084"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9452719.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16315..17259)
                     /locus_tag="IWJ77_001085"
     CDS             complement(16315..17259)
                     /locus_tag="IWJ77_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9452720.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17261..17947)
                     /locus_tag="IWJ77_001086"
     CDS             complement(17261..17947)
                     /locus_tag="IWJ77_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9452721.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18421..18741
                     /locus_tag="IWJ77_001087"
     CDS             18421..18741
                     /locus_tag="IWJ77_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9452722.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18895..19800
                     /locus_tag="IWJ77_001088"
     CDS             18895..19800
                     /locus_tag="IWJ77_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9452723.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19797..20570
                     /locus_tag="IWJ77_001089"
     CDS             19797..20570
                     /locus_tag="IWJ77_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9452724.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20751..22175)
                     /locus_tag="IWJ77_001090"
     CDS             complement(20751..22175)
                     /locus_tag="IWJ77_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9452725.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22386..22832
                     /gene="nrdI"
                     /locus_tag="IWJ77_001091"
     CDS             22386..22832
                     /gene="nrdI"
                     /locus_tag="IWJ77_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9452726.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22897..24879)
                     /locus_tag="IWJ77_001092"
     CDS             complement(22897..24879)
                     /locus_tag="IWJ77_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9452727.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24893..25789)
                     /gene="murQ"
                     /locus_tag="IWJ77_001093"
     CDS             complement(24893..25789)
                     /gene="murQ"
                     /locus_tag="IWJ77_001093"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9452728.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25792..26853)
                     /locus_tag="IWJ77_001094"
     CDS             complement(25792..26853)
                     /locus_tag="IWJ77_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9452729.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(27064..27591)
                     /locus_tag="IWJ77_001095"
     CDS             complement(27064..27591)
                     /locus_tag="IWJ77_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9452730.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(27658..29160)
                     /locus_tag="IWJ77_001096"
     CDS             complement(27658..29160)
                     /locus_tag="IWJ77_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9452731.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29418..30539)
                     /locus_tag="IWJ77_001097"
     CDS             complement(29418..30539)
                     /locus_tag="IWJ77_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9452732.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30665..31606
                     /locus_tag="IWJ77_001098"
     CDS             30665..31606
                     /locus_tag="IWJ77_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9452733.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31756..32652)
                     /locus_tag="IWJ77_001099"
     CDS             complement(31756..32652)
                     /locus_tag="IWJ77_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9452734.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            32921..34261
                     /locus_tag="IWJ77_001100"
     CDS             32921..34261
                     /locus_tag="IWJ77_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9452735.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
BASE COUNT         9968 a         7079 c         6084 g        11319 t
ORIGIN      
        1 tgtgagacaa agctgccatc cataaatact agctgatttt ttcagttaaa tgtctatttt
       61 tttctctgat gatggtattt ccaaaatatt cccagtcatc tggagtcggt ggatttttcc
      121 aaattatctg ttctttatta aatttttcaa gtaaacaatc agataaataa atattcgtac
      181 gagaagtgat tcgactctca atcacaatat tgtaattttt aaatcctttc acttgagaaa
      241 taatgtaatc ggtataagca gatccttgcg aaaaatcaat acagacatga atcggtgctt
      301 caaaatatga taccggacaa ataccgacca ataaaaagag ataatcataa aataagcgaa
      361 tcatgaaacg ttcatctttt cccaagaaat agcgtttatt ctcttgtatg accttttgga
      421 ttgctgtact gaatattgga tacgtctcga agaaaaactg tagctgcttt ttagacgtaa
      481 agctagtcat ctcaaagtcg aatatccaat tttttcgatt gacatgcatc atacccatac
      541 gaagttcttt tcgtatatat agtctttctt cttcagagat ttcaatcgaa aatggtaatt
      601 gatcaatcaa ttccactgac attttttgac taatcatgtg gatatcatga aaatgagctt
      661 ccgcaagtcc aaatgcgcca tctaattcaa attcttcttc cgtgatcaaa aacaacatga
      721 tataagaaat ctcatcaatg atctgctctt tagggatatg aagaaaagaa atatgtgata
      781 aatccttgat gatcttcatt ccttctgagc tcttaaagta ttcatctgaa aagaaacgtt
      841 ttccgattgg ttgattccca taggccctca acaaactaat agtggttagc aaattgaatt
      901 tgattcgttt tgttgcagac agttttaaat tgaacagata ttcaaaatat tgcacgatac
      961 tctttgcttc attcatgacg ctttctctaa atggcataga aaaaccatta aagaccccga
     1021 aatatacccc aaataatacg ttccgtatgt ttatttcttc cccaactaat atgtttttct
     1081 ttaactttat ttgatgctgt tcaagaaaag agactaattc tcttcttttt acataggctc
     1141 ttgatatgct taaaaaatga ttctctgcaa atttttcgat cgttactttt gaatccacga
     1201 tctccgttaa cagtcgagca atttttgatc gttgaaagta gattcttctt aaatttttga
     1261 tatccgtcaa attcagacgt tcgacatgga tctctgcttc ttctgaaatt tggatctctg
     1321 gctttgtttt gaattcagta aattctttat ttaattctat gatataattt cttgttttaa
     1381 atttagacaa acctaatgtt tcatataact taggcagcac tacataattc tttttctcaa
     1441 attctaaata tctagctagc agcagttttt ctctatcttc ctcttctata aggtaatcca
     1501 acttcatatc ctacacctca aaaaatgcct cataccaatt acttgatatg cagatgaaag
     1561 ttcatctttt cacctatact gattttatga tggattatgc ttgttttttc atcagttatt
     1621 ctcatttttt atcttttttt tgagaatatt gcaaaaaatg gaaaaattta tgaggaaata
     1681 ttctatattt tttgttttat tgtgttaaaa tgtacaagat ttgcgccatt tgcgtaaaca
     1741 accacaaaaa agagcaggac ggccaccgtc ctgctctttt acttatttta agttccatta
     1801 cttattaatt ggctaaactt ctgcttgttt ctgatattgc ttgtttttct ccaatgatcc
     1861 gcacttctgt ttctaaagac acatcaaatt tttctttgat tacttcttgg atgtgcgcaa
     1921 tcaattcaat ataatctgtc gctgtcgcat gatcgatatt gacgatgaat ccagcatgtt
     1981 tttcagagac ttgtgcaccg ccccatttca ggccttgcaa tcctgcttgt tggatcagct
     2041 gacctgtata gtgtcctact gggcgcttaa agacacttcc gcatgaaggg tattctaatg
     2101 gctgttttga ttgacgcaat tcggtcaact catccattct tgctttgatt gcttcataat
     2161 cccctgtttg caaactgaat cgagcttcca atacgatagc acgtaattct tggactttac
     2221 tgtgacgata agaaaatacc atttcgtctt ttgtcaaagt tttcaatgtt ccatcttcaa
     2281 gcaaaagatc cacttcagca aaaacatctt tgatctctcc gccatacgca cctgcattca
     2341 tataaaccgc tccaccgaca cttcccggga tgccgcaagc aaattcaaat ccagtcaatg
     2401 attctgccaa agctgcataa gtcgtatcaa tcagtgaggc acctgcttcc gcaatgacca
     2461 tcgttccttt gacatgaatc tgtttcattt cagtcagcat gatgactaca ccgcgaatcc
     2521 cgccatcttg aacgatcaga ttgcttgcat ttcctaatac catccaaggt atatcatgga
     2581 ttcggcaata atcgatgatt tgctttactt cttcttgttt tttcgggaat gcaaggacat
     2641 cagctggtcc acctgttttc gtaaacgtat agttcatcaa tggttcatca aacaatatag
     2701 taatcgtggc taagctttca actatttctt ctttattcac tgaggatttt cctttctttt
     2761 ctcaaacgtc ctcattattc tatcacgctt aatacaatct tgccagtctt gttcaaagtt
     2821 gatgacggta gtgatcttta agcacttcct cacataattc atgacttctt acaacttctt
     2881 tttgtttaag caattctgtt tccgatttac ctaaacggat ctgagaaaag aaggcctcta
     2941 ccattggctc aaacccgcgt ttgactagcg tgtttgtcca gtcgccaaat tctttcactt
     3001 tttttccatc agcctcttga atgacaagct gggtcagatc gtttacttca taagtaccat
     3061 ttttacttgt tacttgatat ttttctatat tagcgccact tgctaaatcc atcgttaaaa
     3121 tagccgtttg attttgtgtt tctaattgta aaatggcata ttccaaaaat tcctttgttt
     3181 ctcttatctg gtattttgtt ctcaaaatgg gttcatctaa taaatacaca gctgtatcaa
     3241 taagatgtaa aaacaaatca taaaggacga actctgtcgt ttcttttgcc gctattcgat
     3301 ttttttgcag gtggatcaac cgcttatctg ggatacgttt caattcttct accataggag
     3361 caaagcgccg gttgaaaccg atcatcaaaa ttatttggtt cttatcagct aattcttgca
     3421 attcacgaac ttcatttaga tggacactca gtggtttgtc aatataaaca tggataccat
     3481 tttctagaca ttttttggct aattcataat gtacttttgt tgctgcgtgg atcatacatg
     3541 cttcgatttt ttcttctaaa agctcatcga tcgtttcata tagatgttgg aacccgtact
     3601 ctttttgaat tctttctttt gtttctttat ttcttgttgc aaaataaaaa tcaagtgttc
     3661 cttgtttttt cgcataagtt ggtaaatatg ctttttctgc gatattccct acaccgatta
     3721 caccaatctt catctacttt cctcctggat catttagtta ctttcatctt acaccatttt
     3781 ttactaaaat gaggtacaat agtatcgcaa aggtaaggaa attaattcga aggagtgtgc
     3841 ttttattgaa atttatctct tggaacgtaa acgggcttcg tgccatcgta aataaaaatt
     3901 ttttagaagt ttttcatgag ttagacgccg attttttctg tctgcaggaa acgaaactcc
     3961 aagcaggaca aattgattta gatcttccag gttattatca atattggaat tacgcggaac
     4021 gaaaaggcta ttccggcaca gcgatttttg ctaaaaaacc agcattgaat gccacttacg
     4081 gaatgggtat cgatatccat gatacagaag gccgtctgat tactttggaa tattccgatt
     4141 tctttcttgt aacctgttac acgccaaatt ctcaaagcga attaaaacgt ttagattacc
     4201 gattagaatg ggaagaagcc ttttataatt atttggaaaa tctaaaaaaa caaaaaccag
     4261 tcatcgtgtg tggggatcta aatgtcgctc atcaaaagat tgatttgaaa aactggaaaa
     4321 cgaatcaaaa aaatgctgga ttcactccag aagaaagagc ggctctttct cgactgttgg
     4381 ataacgggtt tatcgataca ttccgttatt tttatccaac ccaggaaggt gtctactctt
     4441 ggtggaacta ccgattcaat tcaaggaaaa acaatgctgg ttggcggatc gattactttt
     4501 tgacgagcaa agatttagag ccaggactag ctgatgcaaa gatccatacg gacattatgg
     4561 gcagtgatca ttgtccagta gaattagatt tgaaataaag aggttgtgaa aaagtcgttt
     4621 agtttcgagt attaagaaaa attttgtgaa aatggctttc cacattttgc ataaatttgg
     4681 cataataccg agaaactggc ttttgaacac tgtttatcga gagattgtga aaaagctgtc
     4741 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg
     4801 ggcttattac cgcagataca agcttttgaa caccgtttat taagaggttg tgaaaaagct
     4861 gcactgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt tttcgtttgg
     4921 ttgggcttat taccgcagat gcaagctttt gaacaccgtt tattaagagg ttgtgaaaaa
     4981 gctgcactgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt
     5041 tggttggact tattaccgca gatgcaagct tttgaacacc gtttattgag aagttgtgaa
     5101 atcagcgtcc tgacctgagt attagagaaa caaatggcaa aatagttcct catattttgc
     5161 tattttttga gctaataccg agggtcagct gattgaacac cgtttagtgg agaagttgtg
     5221 aaaaagtcgc ctactttcga gtcgtaagaa atttttttga aaatggttcc tcacaacttt
     5281 gcataaattt gacttaatac cgagaagtca tgatccccat ctaactccaa atcacaagga
     5341 atgagttcaa aaactagctt ttctcaacaa atattacata aaaaattcta tgttttttgt
     5401 taaatttatt tgcttttttc aaaagtatgt tacagtaatt tcccaaggag gttttacaga
     5461 aatgaatttt gttgccatgg attttgaaac tgccaatcat cagccttata gtgcttgttc
     5521 tcttgcgcta gtcatggtaa aaaatagcca gatcgttgat gaattctata cattgataca
     5581 gcctgagact ccttttttct ggcgaaatgt ccaaattcac ggaatccatc aagaagacgt
     5641 acgcaatgcg ccgaaattcc cagaagtctg gcaaactatc caaccatatt ttcaaaaaaa
     5701 tcgtttagtc gttgcacata atgctgcttt tgatacaaag gtcttagcgg gatgcttaga
     5761 ttattatcat cttgaacagc ctaattatct ttctttatgt acagtgaaaa gtagccgtcg
     5821 cctgtttcct gaatttccaa atcatcgatt gaacactgtg tgcgaaaatt tgaacatcca
     5881 attagatcat caccatgatg cattggaaga tagtcgtgca tgtgcggaaa tcctgcttta
     5941 tcaagaaaaa aaattcggga cagatccatt gaaaaaactt gtgactataa agtaaaagaa
     6001 tacttatgaa acacacaaag aggtcgtgag cctgccgttt cactccgagt cacaaggagc
     6061 aatttcaaaa aatagcttct catgtttatt aaatattgtg actggtgacc gaggagctgg
     6121 caggagaaca ccgtttattg gagaggttgt gaaaaagctg ttctgcatca agtaataaga
     6181 acagccaaac aaaaatagct tctcatattt ttcatttggt tggacttatt accgcagatg
     6241 caagcttttg gacaccgttt accaagaggt tgtgaaatca gcgttttgac ctgagtatta
     6301 gagaaacaaa cggcaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaagg
     6361 tcagctgatt gaacaccgtt tattcaaaaa aaatggactg cgacacgact tttgtcacag
     6421 tcccttcgtg tgtttttctg ccttctcaaa aggatgacag tttctcactc ttttcctatt
     6481 tcttctattc aatcaaataa ctcattttat atacatctaa aaattcacct agatctgttc
     6541 ttgcccctct aggcatgacc gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg
     6601 ctctttcatt acgaacttga acgtcaagct ccaatcgaaa aagtacaccc atttccttcg
     6661 cccaactgat gatttcttct agcataagag tgcctagtcc cattccccaa tattcctgca
     6721 gaatactgat accgacttct cccacgtgag acaaacgaaa ttgactgtct gccttgacag
     6781 atgctgttcc aataatcgtc ccttcataga ttgcaagcag caataaatta ttattgcttt
     6841 cgtatatata atccaactct tcttctaagg tttctggtgg taacaggagt tctgcttcat
     6901 ccaaaactaa aaattctgtc tccttattaa cttgtcccat catcaaaaga atgtcttttg
     6961 catctcctgg cactgcttcc cgcaagatga tcccaacttc ttcactcatg attttcagcc
     7021 catttttcta gaattcgctg tttgaaggtt tcactttgct ccccgtatga gattagtttc
     7081 acataccgct tttctaaatc tgtatcactt ttttctaaaa tcagttccaa ataatcctcc
     7141 ggcagtgctt cttctaatag atttccccat tcaacaacgg aaagcccatc accttcaaaa
     7201 tattcatcta accctaaatc tgctccgcta gctgctaccc gataaatatc catatgatac
     7261 aacggaatcc gaccttgact atattctcta ataatcgtgt atgtcggact tttgatcatt
     7321 tgcgttatcc ctaatccttg ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga
     7381 tcaccagtca atatcagatt gtcacctggc attgcggaat gaccaattgc ctctgcgaat
     7441 tgttctgtca ttgttaatcc tgatagttcg atcatttttc atcttctccc gctcttcttt
     7501 ctttttgcca gtatatcgcg taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa
     7561 cagcagctgc tgtttttggc tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg
     7621 ctgtgataat cgataattta tatacatctt cttcatttgc tccacgagac aaatcagaaa
     7681 ccggtttgtt caagccttgt agaattggtc cgattgcttc aaagttaccg aaacgctgag
     7741 caattttata gccgatattt cctgactgca attctggaaa tacaaagact gttgcttgac
     7801 cagctacttc tgaatctggc gctttcagtt gtgcaactga aggaacataa gaagcatcaa
     7861 attgcaattc gccgtcgatc atgtattgtg gcgctagttt tttagcgatt ttagttgctt
     7921 caactacttt gtctacttct ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg
     7981 ctacttttgg ttcgatatcg aacaattctg ctgtttttgc agaatctacg gcgatttcag
     8041 ccaattcttg cgcagttgga ttcacgttga tcgcacaatc cgaaaagaca tatttttctt
     8101 gatctcttcc acgtaccatg atcatcgctc cgcttgtccg actgatgcca ggctttgttt
     8161 tgatgatctg aagtgctggg cgaacagtgt cgccagtcga atggatcgct ccagagacca
     8221 ttccgtctgc aatccccata tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa
     8281 tcttttctgc ttgttctttg gatgcctttc catttctacg ttcaacaaaa gtatccacca
     8341 tttcatccca cttatcgtaa ttatcagggc tgatgatcgt aaagctagat gttttgattc
     8401 ctctggcatg cgctgcattt cttatttctt cttcgttccc tactaaaatg ggttccacaa
     8461 gttcttctgc tttcaaccgg gcagctgcgc ctaaaatacg agcatcagta gcttccggga
     8521 aaacgatttt gatcccacgg cgaacaattt taaatcttaa actatcaaac aattccacaa
     8581 gatgacctcc agacgttttt atatgtatta tcatacacta ttccagaaaa aaagaacagt
     8641 ttattttatc tgtatcagtc aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga
     8701 actgcactat aacaggaaag cgctgtttta ttacactgtt tcaggcagtt gccaatcaat
     8761 cggttcttct cctaacgcaa ggagcgcatc attcgttttc gagaagggtt tggaaccaaa
     8821 aaagccacga cttgcagata acggactggg atgtggagat gttatgatga tatggcgact
     8881 cttatcgatc attttgattt tttcttgtgc cggttttccc cacaagataa acacgattgg
     8941 tttttcccgt tcatttaatt tttcaatgat cgtgtctgtt aatctttccc aacctttccc
     9001 gcgatgagaa tacgcttgcc cttcccgaac ggtaagaacg gtgttgagta aaaggactcc
     9061 ctgtttagcc catgacacta gatttccatg ttgaacaggt gcgattccaa gatcatcata
     9121 aagctcttta tagatatttc ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa
     9181 agataagcca tgagcttgat tttctccatg ataaggatct tgtcctaaaa tcaccacttt
     9241 aacttcttca taaggagtca attccaatgc ttcatagata tgatacatat ctggatggat
     9301 cttttgggac gcatattctt tttttaaaaa ttctcttaga tgcagataat aatcttttga
     9361 aaattctgag gacaaaactt cttgccaact gttatgaatg atttctttca aaaactccac
     9421 ctcctaataa aaagaataac tcacattttc ttcaatcaca agggacaatc ttcaccctca
     9481 gcacttgatt aatatggtaa actaggaaca agaagcagaa aggaacgata atcattatga
     9541 tcaaattaat tgcatcagat atggacggca cattattaga tgcccatatg agtgtttctc
     9601 ctgaaaatgc cgaggcgata cgttttgcaa atgatgccgg tgttgagttc atggtcgcta
     9661 ctggtcgaaa ctatcaagaa gctcgcgcag ctttagatga ggtaggaatc gattgtgcga
     9721 tgatcacttt gaacggggca caggtatttg ataaagaagg aaattcattg tttaccgtgt
     9781 cgattccaaa taatcaagca atctctgttt tagatattct agatgctaac ggtatttact
     9841 atgaagtagc aaccaacgat ggtctttatt ctgaaaatca agcgaaacga attgaaagtt
     9901 ttgcttccat ggtggctacc catttacctc accttacgta taaaatggcg attgccatgg
     9961 cctcagctaa gctggagtta ttgcacatca catacgtgga cagtattcgt gctattttgg
    10021 aagaaaagaa tctggaagtt ttaaaaatta tttgttttca tacagaaggg ccagctgtat
    10081 taggtccagt tggaaagcag atttctcttt taggcgatct agccgttact tcctctggtc
    10141 aaaacaatat cgaagtgaac cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg
    10201 ctcacgagcg tgggatttct ttagaagaag tcatgacgat tggcgataac ttcaatgatg
    10261 tcagcatgct gcaagtagca ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa
    10321 aagattatgc caaatataca acggacacaa accttgaatc tggcgtagga aaagccatat
    10381 tgcgcgcgat tgcagaagac ttgtagtttt atctagaaaa gagggatata gatgtcagaa
    10441 tttttcattc aggaaaagca gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa
    10501 ggacattccc tgtttttgat ggttggtcgc tggggaacaa agggcgatgc attgtctctt
    10561 tatgccatga atggcgatct cgttgctcac atcaagcaga taagcctgac attcggtacg
    10621 cgttttgagc tgtataaaga gttcgaaaag gtgggaacca tgcagaaaat attcaattgg
    10681 cccggtgact tttattatat tcgtagactt cactggacag tccaaggtga tatctataat
    10741 catcaatatc aaatccatca tttcaatcag caaatcatga aaatggataa agcaacactt
    10801 ttttcaggag attactatgt attggatgtc tcaaatgatg aagatgctcc agtttgtatt
    10861 tgtattgctg cggttttaga ttactggctc taccgtaaag acaaaagtcg cgataagcct
    10921 gtttcttacc gacttaactt aagttaaaaa gatgtacctg aaatgcggaa atacgcattt
    10981 caggtacatc tttttagata ttcttttgca tatgatcata aagatgacca ctgatcatcc
    11041 gctgaccgac aactttgaag tcatggcgtt cgtataattt tttagccttt ggattttttt
    11101 catccacact tagaccgatg acggagcgat tagctttctt cgccaattta ggtaatgctt
    11161 ctaataatcg cgaaccgatg ccttgtcctc taaaatcttc tgatacactg atagagtcca
    11221 aataccattc gttgggaaac gtttctttat caatgaacaa acgtgtagac gcatctagct
    11281 gatatttctc taagataggt accagtggct gatcgatgat tgattcttct tcatctgtat
    11341 aaccgtaagc aactcctgca actttaccgt ctatttcttc tacgatacct cgtgaataac
    11401 tgtaacgata atttggatca gtgattgctt cttctaaaat ctctaaagtc ttttgttccc
    11461 ctgtctgctt caaaaatggc agttccatat cttttagaat cactaagatc aatggggcga
    11521 tttctggagc atcttctttt ctcgcaaaac gaatcatagt ttcctctcct tttctccatt
    11581 ctaacatgca gaaaaagaaa tcgcaaaatc gttattttaa aaaatcttca atcttttcag
    11641 taatgaaata accatgagta acgattcttg ccgcttcagt tcggcaataa gggcagacga
    11701 ctggcatttc tttatcatta tgttcgacta cattattgac ttcttcgtat gcaaattttt
    11761 ttccgcaatt agggcatttc attttttcca tgttatctca acttcctttc atctttactc
    11821 tcttagctta ctcttatatt gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc
    11881 catacctgac ggtcctgttc tccatctatt aatcggactt tataataagg acgttcatct
    11941 ttgactagaa gtgtccactc tttcactttt gcttttttcc cgtagtagct gactgcttcc
    12001 ttcatcgctg cttgcggttg tttgaactct tccgtttttt ctatatcaaa aagatgttct
    12061 gcggcctttt ccgtgtcatt tcgtacggtt gttttcccag tggatggatt gactgctatg
    12121 acttgtcctt cacaaaaaaa ctggtattca tatgttgcat tcgtaggatt ctccaaatct
    12181 ttttcaactg caaaatgaat agcagatatg ttatgttctt ttgtttcgtt ttttacgatt
    12241 gctgctgctt ttttcaatga gatccgatat tcctggtcac taatatcatc catgtgaaaa
    12301 gcggcattga gcagtcccgt cataccggta atccctatta cgatgagggc agcaacacca
    12361 aacatcattt ttttcattct tcattctcct gtttcttttt tttaaagaat atcaagaaat
    12421 gaacgaatga aaaatcgaat gaaacgaaaa aaaagtgaca attttgttat ctttattcgt
    12481 gcagttctaa tggcaatccg tctggatcaa agaaaaacgt catttttttt cctgtaaagg
    12541 tatcttctct aatcggttct gattcgatcc ctaaggactg caattctgca attgtttctt
    12601 caatatcgtc tactttgaaa gcaagatgac gcaagccaca agcttctgga tagtttggtc
    12661 gttgtggcgc attagcaatt ccaaaaatct ccaattcagc acttcctagt ttcagatcta
    12721 atttatagtc atctcgttct ttgcggtaat tttctctgat tacttcaaat cctaatttat
    12781 taacataaaa atcacgtgat ttctgataat ctgaaacaat gatggctata tgatggatcg
    12841 ttgtaaggtt catatgagca ctcctattct tcgtttaagc tcggatctat attaatctcc
    12901 agttccattg taaatgtgtc agattgaaaa aaatagctgt ttttcctttt tataagaaaa
    12961 aaggcaaaaa aaacagaaag taggtcctcc aagacactct tcctgttccc aatataataa
    13021 atgaaagtaa gaaattatat tttcaagaaa acaaacagaa aattatcata acacaaatta
    13081 tttataattg aaagactagt tttttttcaa gaaaagcata caatagattg tgttagtaaa
    13141 tacagaacac actacattta gtgtcttaag gagtcctcat acatgaagtc atattttcag
    13201 tttttagctc atcagctaaa aggctggccg caacaaaatt attatctttt ctttttcagt
    13261 cttggctgcc aggtcatgac tcttgtcaat agtcccatca cttggctgtc cgttattaca
    13321 tttatcggta ccacgcttgg tgtattgtgt attttatcca tcaatgcggc aaaatcagtc
    13381 aacggtattt taggtatttt gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag
    13441 aactatttaa gtatcggtga acagattgct tatatgatca cattggatat ccccgtattg
    13501 ttaagtaaag aatggaatca taatatggct tcaaaaatcc gaaaattcac tggaaaaagc
    13561 tggtttatcg caatcatcag tactttcctt gtctatcttg tttcaggatt cctgattggt
    13621 cgttatacag atgatcctcg gccgtgggta gatgccattg ctttttctat tagcttgact
    13681 gctggtatca tcagtttctt gcgttacaac aaccaatatt actggtggtt agcttcaggg
    13741 cttgctcaaa tggtcctttg gtatatttca tttacgcatg gatctgcaag tttggcgatg
    13801 ttcgtgaaca gctctgttta cttgctgaat gatgtccttg catttaccgt ttcaccttgg
    13861 tataaccaaa aagaacgtga acgtatgcaa aaaatggaag aagaatacta caacgcacaa
    13921 caacacgcat aaaaaaactg agcgtgggac aaaagtaaag aacacttttg tctcacgctc
    13981 taaacacgta taaacggcgg aatcagaagc aactccttcg gaaataagct gaaattcaca
    14041 aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt
    14101 cccagcctct attttttata gtaattcctt cagatacttt cttgcctcat cattgaaacg
    14161 cagtgattct tctaacttct gataatattc caacgcttgt ttctgagctt cagtaaaacg
    14221 ataatgtttt cctttccatt tgtcgattgt ctcttgcaca tttccagatt ggatatcttt
    14281 cactactagt tgccattttt cttttgagac tgtacggtct ttcttatcag cagattgtat
    14341 agcttctcgt tctttacgca atacttttaa aaaaccattt ctgactatcc gaggaatgct
    14401 gtcgaagaac ccttctgtaa aaccatagcg acaatattgc ttccaaataa atagatagat
    14461 ttttggttgg atgaatcgtt ggtttttcgc aaaatattcc atctgagcag tgacttctgg
    14521 tgttcgttga cgttttgtct ttctcaacac ggaaataaat gtttcaaaat aagcattagg
    14581 atgtactggc acttcttcat atacgatgga ttgccaatct ttacgtaaaa atgcgcgata
    14641 gttgatcaag aattcttcta catcctttac taattcttct gcaaagtcgt caaatagttc
    14701 tttttttatc tgtcgctttt ttgactgccc attgatgtgt tcacttattt tcttgatttc
    14761 attttccaat gcttgataat ccgctatgta aatcggcatt tctacttggc gaaattctgc
    14821 gagccgctga gctaatttta tatccggtaa atatggctgt ttcatccgac gattttggta
    14881 caagacaaaa cagtattttt gccatagtcc ttcaggaaaa tcgatattct tctgcttaag
    14941 ccataataat tcgtctaagg ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc
    15001 cactgtcgga gagacatgca ggtgatcggc aaagtggttg atgcctagtt ttaggatctt
    15061 tccggttttt ggcgatttaa cgatgtattg cacttttaat tctctaccac actcacaata
    15121 caatttttgc tggcttcgtt ttgttcgaaa aaacggattg atcttgaagt cagaaaacac
    15181 ccaatctgtt gcagctaaat aatgttcttc cataaaacga gagctgatta gatatcggcg
    15241 gtgcttatcg actagttccc gctgtttttt cgttaatttt gcatagatct cccaacgttg
    15301 actgcttgtg tattgtttag aaattttgat gggatagctt ttgctgattt cttttggaaa
    15361 ccattcttca aagacggatt gccattcttc cacggctgtt tctttccttt ctaattacaa
    15421 aaagctggct actaacataa cgttagcaac cagccagtgt ttcttgctaa attagtgagt
    15481 tgttccggca tttccggaaa tatcttcttt attttctact gctgcttcaa ctgcataagt
    15541 caaagaagaa gcaatgtctt ctaaagacat agaaggcgta cccactggac gatcgatcac
    15601 ttgatcgggt gagaatggca cgtggatgaa cccacctcga attgccggga attgtgtttc
    15661 gatcaaatga agcaaacgat acatgatatc attgcagaca aatgttcctg ctgtataaga
    15721 gatgtacgct ggtagaccat gatcttgtac attttttgtc attgctttga ttggcaatga
    15781 ggtaaagtaa gcggctggac cgttttcttc taaagccgta tcgaatggct gatttccttc
    15841 attatcaggg attcttgctt ctgctagatt gattgcgaca cgttcgaacg agactgctga
    15901 ccgcccacct gcttgtccga cacagatcac gacatctggt tggtgctgtt ggatggcttc
    15961 tttgactact tgactgcttt tttcgaatac ggtcggtact tccactttga tgatttcagc
    16021 accgctgatt tcatctggca ttttttttac tgcttcatag gctggattca ctttgtcccc
    16081 accaaatgga tcaaaacctg ttactagtac tttcatctgt tgttcctccg tccgattgat
    16141 ttaacatatg gttatctttt atcataccat tttccttgaa cagcgtaaat aaaggaaaca
    16201 atagtttaaa taacataaca gatcaaactt aatatattct tataaaaaaa tagtgttcca
    16261 ctacaatgtt agaagaggtt gcgacaatta ttctgtcaca acccctctct tctattattt
    16321 aaatagaatc atataagcaa tttggaaaac tagcataaat aacgcaataa acaactggtt
    16381 tttgatcact ccgtatttgt ctttcatctc cagcatcgct acaggcacga tattgaagtt
    16441 agcagccatc ggtgtcagca gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat
    16501 taacgctgga ttcgctccat gactaaatac gaatggtgca ccgatcccaa ccgtcatcac
    16561 ggtgatggcc gcaaatgcat tccccatgat taccgtaaat atcaccatgc caagagcgta
    16621 aatgatgatg ccgatgttca catttccttc tggaacgatc gtacctactg cattggaaat
    16681 cactgtccca actccagcgc ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa
    16741 catgcttaac ggccctactg tgcccagcat atctgctgca tcatctaaaa atgtacttgg
    16801 tcgattatct cgagaaaaga acatcaagat tagcatggct gcaaaaacac cgacgcccac
    16861 gccgactaaa gcacctaaat tggtaaatag agcaaacaag atcgcaaaga caccgatact
    16921 caaagctgga ataaaaattt tcattcctag tttgtctgac attttttcca tatattcttt
    16981 tgatggaagc cggctttctc ctttgctgac tttcttagca attgctggta tggtcatcgc
    17041 aaagatcaag ccaccgttca cgattgccgg aagccatctt cctcctgcaa taactacccc
    17101 taagaccgtc cagaaaaagg ctgacccata acgatgagga ttggttttat ctgttaaatt
    17161 tcttactcca gtgtaaatca atacgagtcc catcacgata taaaggatct ccagtaattt
    17221 atcgcctaat aaaatatcag gatttgtaaa aaagtccatc ttattgcccc tttcctggat
    17281 aatatttctt cgctaatttc ttttctttaa gatagaagta gacacttgcc acaactaaag
    17341 ccgtgatagc cactggaact tccactaaag caagctgtcc caatgttacg tcatatccta
    17401 gatctttcag tgttgattgg actagcaacg cccctgctcc tccgatgaat agtacttggc
    17461 caaagaacca gcagacattt tccatagctg aagccatacc ttttagttct tcttcatgct
    17521 taggatggac ttctaaccct tttgattcga tggtccccaa tgccatcggt aaaatgattg
    17581 gacgaacaaa tccagcaact ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa
    17641 agatcccata aatatctaca atcataccag cagatacttt cttgaatcgc ttgatcaagg
    17701 aagccgctga ttctttcaga ccatttcttt caagcgttcc tgttgccaac ataatgatga
    17761 taaaaatcgc catcccgcga ttgtttacaa aacttgtacc taaaagttct aacattcctt
    17821 gaatgccaag accgccagtc aaagctgtaa caactaaagc aaccattacg atcaatatcg
    17881 agtctaattt aaatgcaaac ccgagaataa caatcaagac gcctaaaagg ctaagataat
    17941 tttccatata ttttcctcca taaaatgaca atattttttt aattataaag caaaacacat
    18001 aaaaaatata gttgttttct cacgaaatag acactatttt taaatatttt tcgttatttt
    18061 tttatcacaa aaattatctt acttattgta aggtaaagct ttttttaatt tattgcccga
    18121 taatatatag aaataaggtc tagttactgt tttttttaaa tgattaataa ataacaactt
    18181 ctttctatga aattgcaaag cattcatctt cttttccgct aacgatatat ccgtcagtgc
    18241 ttggtacact ttactaaaaa gtatgagatt gccgatatct ggataggtgt gctatactac
    18301 agaaaaagat atacaatctg ccaccgggta ggataaagag gatgatcatt ccattaggtc
    18361 tttgaaggga tgattttcct ctttttgtgt atttttttat agttaaggag gaatgtaagt
    18421 atggcatgga tggaattaat catagcagga attttggaag tattctggtc aacgatgatg
    18481 aaatggagtg atggtttttc taaaatcaat tattcttttt acacagtgat tggcatgatt
    18541 gctagttttt actttttgtc aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct
    18601 atctggacag ggattggcgc agttggttcg gtcataattg gtgtggtctt gtttcatgat
    18661 aagctgaatc tttccacttg gttctttgtg gggatgctac tcatcagtat cattggcatc
    18721 aagattacta gcggccacta gacacttgga ataagtactg atcagaaaaa ataagccttt
    18781 ttatttaata ttcaattttt ttatgaaaaa ataagaaata agtccaatga cctagcgact
    18841 tctccactca tcaggtgtat gcttgtacta tctatagaaa aggagtgaat atttttgaaa
    18901 agtctttcgg tccaagaatt atccaagaac tttggtcaca aacaagccct cagcgatgtc
    18961 agttttagct tatcccctgg caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa
    19021 acaacgatca tgaaagctat tttaggactg atccattact cagatggaac aatctgtatt
    19081 ggtgaaaatg agatttctcc ctctagtcat aaagggttgg aacaagtagg agcattgatc
    19141 gaatacccgg gtatctatcc attcttaaca ggctatgatc atctgaagtt attctctgaa
    19201 accaacgatg tatctgctat cgacactatc gtgaatcagc tcaagatgga aaagtacatt
    19261 cacaaaaaag cgaaatccta ctcgttggga atgaaacaaa aattgggaat tgctcttgcc
    19321 ttactgaacc atccgtctat cgttatttta gatgagccga tgaacggttt ggatccacaa
    19381 gcaacaagag atgtgcgaaa aatcattacc gacttggcta ctcagggcac ctctttcttg
    19441 atctccagtc acttactcag cgagcttgaa aaaatggtcg atgagctgat cctgatcaat
    19501 caaggaaaga tcatcaaaca atgtacaatg gctgaattga accaaacagc gcaagatttc
    19561 atcgttatct ctacaacaaa tgatctagct gctcgaacga tcttgacaga agctggatat
    19621 actttggaag atcctgataa aatcaagctt ttaaaagaaa acgaagaatt attaggaacc
    19681 gtgatccagc tattgtctgc acaaaatatc catgtgacag acgtccagca tctgacgaat
    19741 gatttggaat cttctctatt ggaaattctg gattcagtaa agggggaaga agaaaaatga
    19801 cattgcttgt aaaacaagaa ttatttaaat tgatcaagaa aaagtcaact gctgttttat
    19861 ctgttctatt agttgttttg ctcataggaa cagcacttct agccaaaaaa tacacgacga
    19921 tcattgatcc agttgaaatg actgctcagc tttttagtgc cacatcttgg attgtcttta
    19981 tcatgattgc agctgcaagc acgatcattt cgatggaagc acaatatggt acattgaaaa
    20041 atctccttta tcgaaagtat tctcgaggag aaatattagt aagcaaatgg atcacattag
    20101 ttatttattc tgtttattta taccttttag ctattattgt cactgtgttg atgaaactga
    20161 ttcttttccc ttcgatttcc ttcacggaga aggtttctac tggtcaaaca ctgatccaaa
    20221 gcttgttcac ttatactttg ggtagttata tcggtttatg gctgatttta agtttagtgt
    20281 tgatgattgc ttgctttatc aatagttccg gcgcatccat ctcagcagga atcgtctttt
    20341 attttgcttc gtcaatcata tctggtatcc ttattgcact catccaaaaa tgggagtgga
    20401 tcaaatggaa tccgatcagt atgttgaatc tgcaaaacca aattggaaac gaagagatca
    20461 tgaagcaatt gactcatctt tctactaatc aaatgctatt cggtaattta gcttacatcg
    20521 ttttgttctt agcattagga tatctcgtat ttaagcgaaa aaatgtatga tgactcatca
    20581 aaaaagaggc tgtgaaagag acgttcagcc ttattgccga ttggctgtct cttaaacacc
    20641 gtttattctg aaaaaggagc tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt
    20701 tttgaataaa cgacaggctc acaacctctc tttatacttc ttttatactt ctatacgtga
    20761 agcatcccag tggcaaataa aaccattgca atcactccga ccatcgtcac acctataagt
    20821 aaaatcttat ctaagtggat atgttgtaca ggctcgtttt gctccttacg tgctttgagg
    20881 taaaaataaa tgcctggcaa gaaaagaaca agaacaagca ttacttcatt aaaggctacg
    20941 actagcatag cccatacttg aaaggcactt gctaaaagcc ctactaaaaa ttggacccat
    21001 tctttccgct gataagaaag tttcatttgg tacaatccta agaaaagcca cgtgaagaaa
    21061 atagctgctg taccaagtgt gtaagcaaag ttataagcac tgtcagtgaa taataatgaa
    21121 agtaaaaaga cattcgtaca taacgtcgtc aatacgatag cataagttgg tgccccattt
    21181 ttgttgagtt ttccccatgc tttaggcaaa agtccgtctt cagccatgtt tcccattgtt
    21241 tcaatcggta agatcgtcca tgagagccaa acaccaacta aagaaatgat caaaccgccg
    21301 ttgatcacga ctgctcccca tttaccaacc atcatttcaa acacatagcc catagcaggc
    21361 tgcgccttga tcgtttgcag ctgattcatc gtcattgtcc catatggaag catggaaacc
    21421 aagacgtaaa taataatcaa gccgaatatc cctagaatcg ttgctttccc aacatctgat
    21481 tgtttttttg ctcgtgaacc tacgacagag gctccttcaa taccaacaaa tacccaaagc
    21541 atcaccatga tgcaactcaa tacttgactg gaaaacggga tcggctgtcc attcgcattc
    21601 aatataatgt cgctagaaaa gttatcaatg aattgttgga tcgaaaacgt gctgatagct
    21661 actacaataa ataaaaataa tggcactaat ttacagaatg tgacgatagc attgacgaaa
    21721 ctagcatttt cgactccccg attcactagc catgcatatc cccataaaaa gactgtactc
    21781 atcacgatcg atgccagatt ttgcccacct tcaaatactg ggaagaaata tcctaatgcg
    21841 ctcatcaaca atgttgcaaa agctacgttg cctaaccagc atgtgagcca gtaaccccaa
    21901 ccgctgataa aaccagcgaa acggccaaaa ccttcttctg cgtaactaaa gatcccgcct
    21961 tttaaatctg gacgtttttt attgatatta ttgatcgaca aggagagcat caacattgct
    22021 gttcctacga gtacccaact gaaaatagct gggccgccag ccgatccgga gatgtccgtc
    22081 ataataccaa atacgccacc tccaatcgct gaaccaatta ctaaggcagc aagtggaatt
    22141 aaaccaattc cttttttctg ttgctctgta tccatgtaag catcctccaa aataaaaccg
    22201 cttttatcaa aaagtataac aaaccttttt tatttttact taattaataa aaactttaat
    22261 agaaatataa ttctaccggc ttttttctca ttatacaagc aaagcgccta gtccctttcc
    22321 aaattacacc accaaaaaac ttaaaaaaga tgctatacta aaaggaaaga attggaggat
    22381 gatccatgaa cattttatat atatcgattt caggaaatac gcgtgccttt gccaaacatc
    22441 tagcagaata tgctgaaaaa atgcatgcag aagatcctgt caatcctgaa gttactttaa
    22501 aagaaatcca tgaaaatagt gattttacga aagaaacaga accatttttt actttcgttc
    22561 ctacttattt agacggcgga aatggtttag ataacggtga tacagaaatc ttgaccgaaa
    22621 cgatgcgtga atatctggaa catgaagaca atcataaact ttgtcttggt gtagtcggta
    22681 gcggaaataa aaacttcaat aatcagtatt gtctaacagc caaacaatat gcacaaacat
    22741 ttggctttcc tttcttggca gattatgaat tacggggaac accatctgat gtgacacgaa
    22801 tttatcagat tttagcagaa aacaataaat aataaaaaag aagaagctga aaaatagatt
    22861 tcagtttctt ctttcgtttc tattgtttct atcatattaa gctttgacat ccatcacagg
    22921 tgcttttgct tttgctttac ctgttttttc caagacaaag ttttttacac gatccatatt
    22981 ggttaccaag acgatcatgg atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc
    23041 ttctgctaca agcgtatcag gtgtcacttg ctgcccttct gttactttca catcaaatgg
    23101 cgtaccgcct aagtcaactg tgttgattcc catatgaagc aagatttcta aaccatttgg
    23161 cgttgtgatc ccaatagcgt gtttcgttgg gaagacagag ctgactaccc cagtaactgg
    23221 agaatagata tgtccgtttt ctggttctac aaagtagcct tcacccatca tcttttgtgc
    23281 aaaaactgga tcacttgctt tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag
    23341 gatagtcaca ctttcagctg aagtttgagg tactactttt tcaggcactt catttactgt
    23401 ggctgtttca gtcgctaatt cttctttttc taaacggtct ttaggaatac cgaagaagaa
    23461 tgtagcaacg aagccaccag cataagctgc caataatcca aggatgtagc ctaaccattt
    23521 accgtcagag atcaatggga tcaaagcggc tcctgaaggt ccaatagcga tagccccgac
    23581 atttccgatc attccgatca ctgccccgcc gatacctcca ccgatacaag cagtaataaa
    23641 tgggcgtcct agaggtaatg ttactccgta aatcaatggt tcgccgattc ctaggatccc
    23701 gactggtaaa gctcctttga tcatttctgt taattcttta tcttttttgc agcgaatcca
    23761 taatgccaat gctgcaccca cttgtcctgc accagccatc gcaagaattg gaagcaatag
    23821 cgtacgacca gtttcatcaa tcatttgcac atggattgga gtcaaaattt gatgcagtcc
    23881 gaacataacc atcggcaaga ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac
    23941 gccaagtacc cagttgatcc caccgaccat gccatcagaa atgaaacctg ctaaaggcat
    24001 gatgaagaaa atttcaatca gaccgatggc aatcaatgaa agagttggtg taacgatgat
    24061 atctaccgca tcaggtacta ctttatgcag ttttttctca acgattgaca atagccaaac
    24121 agcgaagatg acacctagga tcccaccttg tccagctgca agagcttcac cagtaaataa
    24181 gtttttcagt ggtgcttccg gattcatccc tgtcagcatc acaacggccc cgataactcc
    24241 accaagagtt ggtgttgcac caaaaacttg tgcagcattg atacctgtat agataactag
    24301 gtagctaaac atcccgtttt tcaagatgtt catcacatcg atatattgtt gccatgttgc
    24361 tgcatcaagt gttccggctg tgactaagtt tgctaaaact gcggcaatcc ctgccacgat
    24421 cccagtaccg acaaatgcag gaatcattgg cacaaagata ttagaaatgt cttttaatac
    24481 cgatttgatt ttagatggtt tttgtttcga tttttgtgcc gctttcattt cttgtgcccg
    24541 ttcgtttaca cggtcttttc cagatgctga tgaatttgct gtagctgtac ttacagaaga
    24601 aagtttttct cctaatttca cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac
    24661 tttcccaggt ccgatgatga cttgaagctg ttcatcttct actacaccaa gcactcctgg
    24721 aatcgctttt aattcagaga catttacaag atcatcattg atgatcttca tacgcacacg
    24781 agtcatgcag tggacaatgc tttctacatt gcccatgccg cctgcccctt tataaatttg
    24841 ttttgccagt tcttccaaag tcagttcgtt ttttgccatg gtcttttccc cctcataagg
    24901 tcccttttac aaatccgttt gcttcagcta attttgcttg agcttcctct ttatcactat
    24961 ttgtcaaaat catgacgata gccagcttaa cattttgatc tgcttcttca aagtactttt
    25021 cagctgtttc gtaatcagct tctgtcgctt gcatgatgat tcgttttgag cgttcgatta
    25081 gtttttcatt tgttggtttg acatcgacca tcaagttatt gaatacttta cctataccaa
    25141 tcatactgat agtagaaatc atattcaaga taagtttttg tgctgttcct gacttcaatc
    25201 gagtagagcc tgtcaaaaat tctggacctg catcgacttc gattgggaaa tccgcgtgtt
    25261 tactgatttc ggcttgttta ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg
    25321 cgtaatctaa tccaccgatc acatatggtg tacgaccgct agctgcgata ccaataacta
    25381 tatcattttt tgtcaaattc agcttgatta gatcttcttg ccctagctca taagaatctt
    25441 cggccccttc gacagctaca gtcattgctt cttctccacc agcaattaga cctatgacca
    25501 tttccggttc tacaccgaat gtcggtacgc attcagcagc atctaacaca cctagtcttc
    25561 cacttgttcc ggctcccata taaatcagac gtccaccttt tttaaaggac tcaatcgtgt
    25621 tagcaatcac tggttcgata cttgtcagtt ctttttcaac cgcagcggcc actttctgat
    25681 cttctttgtt cattagttga acggcttgag aaatactcat ctcatctaat ccaaaagttt
    25741 cttggtttct gcgttccgtt gttaggttct ctaaattgat tttgctcatt tttatcttcc
    25801 ttcttttctt ataaattgat agttcatacc agctttgatt tggttgatca atggtagctc
    25861 ttctttgata actttagcta ctacattcac tttttcatcc gctggcaaat catatttggt
    25921 taattggatc tcacccatat agcgcaaata cttttcgtta tctaaagtaa tagacccttt
    25981 catacgcgta gctgtatttc ttgctgggat aggcggaatc tctcgaaaac gtgcatctac
    26041 gcttcgtatg acatctcttg catcgtcttg ccgattcgta tgcttcccta atatatattc
    26101 gtaaaattct tcatctacag gttccgtatg gagcaagact gtctgttctt tttggaaaga
    26161 agcaaattgc tcctgaacgt tttctgacag acctgcatct ccgatgtaaa caatatccgt
    26221 gttacttgct aatagatcta gagcgcctgc aagaggatgg atccctcgat gttcttccaa
    26281 agttggcaat ccttgatata gtgggcctct caactctgta tctcctggta caaaaccttg
    26341 gacaaggaga ccttgaattt tgagccattg attttttttc tgataccatt ctttgtccag
    26401 ccctgtttcg ggacgtggat aataattatg ccaagcttca atctgtgaga aatcagcttc
    26461 tgcttctttc aattcatcga tatctttagg cgtgatcgtg ctagcattca aactgatttt
    26521 caattgatgt gaccagtcgg caatctgctg attggaaata tgataatcca ttcttaaacc
    26581 tgttacacca attgctttca aaggcgttag atcttctaat gaaaaacccg ctcgttccaa
    26641 tgcctctcct gaaatatcga ccatcaattc tagtttattc tttttcgctt cgcttcctag
    26701 ttcgatcaat cgcttttgat atgccgaaac atcatcttct ggtatatgca tggaagtgaa
    26761 aataccgaca aagccattgg atgccatttg gcggatatac tgtttcttct cttctgacaa
    26821 agaatcgttc ataaatattg aaaagccaaa catattatcc ctctttttct gtatccgtct
    26881 tcatttctat gactagagta tatatctaaa gaaacaaaat ttcaatactt ttattaaaat
    26941 aattaaattt catttctaaa ggttgagttt atattatcaa aagaattgtc ataacctaaa
    27001 aaaggcactg ctaagcaaaa gcagtgcctt gaaatatttt atttaaataa cacaaaaaca
    27061 taattactca taagaaacca attctgtatt atctaccatg aatgtaaaga acccctcatt
    27121 aggcatcgga tgaaaataag cattgatcac tcttatcaag gcattatggc aaactaacag
    27181 atagacattt tcttcgtctt cgatgcattc tttcaataaa ggaacgatcc gtgcatacac
    27241 atctaagact gactccccat ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa
    27301 gttctcatct ttgcttggca gtccatcata atccccaaaa tccatttcta ccaatcgttc
    27361 atcaacggtc agcggcaagc tgagacgatc agcaactgct tgtgctgtat ctcttgctcg
    27421 ttgaagaggg gaatggatga ttttcgtgat tggaacaggc aaatctacta ccttttctgc
    27481 tagtttctgt gcctgcatct gtcctttttc agtcagcggc acatctgccc gtccgcaaac
    27541 taatccttgt gcgttccaag ttgtttctcc gtgtctcgtt acataaagca tgtgattctc
    27601 tccttcgttg gcaaagttcc ccttaaccat accttgttgt tcttgagatt acaagggtta
    27661 gattctctca agagaaataa tctcagtaga tactttatcc aggaaggttt gatcatgttc
    27721 cactagtaac atggcaggtt tcacgttcaa gatcagttgt tctaattgtt cttgattgaa
    27781 gacatccaaa taattcaatg gttcatccca tgtatatagt tcagctggct gtgataaaga
    27841 tttagccaat tccacttttt tccgttggcc catactcatc tgctcgatct tgttatgaaa
    27901 aacatctctt tccatcccaa gctttcggag gttgttcaaa aatgcttggt agtctacttg
    27961 gtttttctct gcaaattccg ccaacgttcc tcgattgtct tcataatttt ggcttgcata
    28021 actaatgctc agatgttttg gctggtattt ttctcctata accttgccgt tgaatgcccc
    28081 cagaagataa tggataatgg atgactttcc tgcaccgtta ggacctgaaa tcgccacccg
    28141 ctgatgaggc tcgattgtaa aatgaattgg ctcgaataac agattttcat accctaattg
    28201 aagatcttct acgcttaaaa gtcgcttatg gtgagacgct tggctattca tcgtcaaaga
    28261 atcgatatat tcgatatctt ttaatagttt ttctttctcg ctgatctggg tctccatccg
    28321 attcacgatt gctttggatc gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt
    28381 attcactcgt ctagattcag tatcgatgaa tccgacttgt ttctttgttt tatctccttc
    28441 tcgggaacga gaccattcgg ctttttcagc tgctgttttc tttagcctac tgacttcttt
    28501 tttcaatttt tcattttgag ccatttcaaa ttcatcacga agttttttct gttcttcata
    28561 gatagagaaa ttcccttgat aaagttccag ttgacttttt tcgattgcta aaacatggtc
    28621 cactacttca tcgataaatc cccggtcatg gctgaccacg atgaagcctt gttttttctt
    28681 tttcaaataa gccgctactt gttttctacc agagatatcc aaatgattcg ttggttcatc
    28741 gattaacggg aaatgagtgt catccacaaa taaaagtgcc agtaggactt ttgtcttctc
    28801 tccccccgat agtgtgctga attctctcca taagatttca ggatctgttt gcatcaattg
    28861 gagctctctt tcgatttccc atatctcaaa atccgtaata tcatttaaca cgtaataggt
    28921 taaacgttct ttatcttttg tctgttgcgg gaaataggca aattcttgct gatggattac
    28981 ttgcccttga taaggtagtt tgttttgtag aatattcagt aaagttgtct ttcctcgacc
    29041 gtttcgtccg ataagtccta gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa
    29101 taataatgtg ccttggctgt cgtagccgaa tgtcagattt tttatttcga ttttagacat
    29161 tttccatcac ctcgtaaaaa aagaccagtc ctattttcag aactggctaa agaggatgca
    29221 agggttggat acacacaaaa agacccatac atcgaaaaaa ccaagccaat cccgaaaatc
    29281 tgcacactgc ttccttgtct ccacaaggta cagtctgaat gcagctttca tcaatcgact
    29341 taatttttca atgcacggat ccctcacttt gtcctatatt tgaaattacg atagcataaa
    29401 gaaaatgatt ttgtcaacta cttcgggagg atggtgaaaa atctgctaag tgtctgtcct
    29461 aagatttttg ttttttctaa tgtttgcctg tgttccattg cttcttctaa acctattgga
    29521 cccaaatgaa tacttaagac aattgggaga aaatcagaaa gtccttctag ctcttggtct
    29581 ctacttccgc aaatagcgat tattggtttt cgatattttt ttgcaagcac tgctacaccg
    29641 tatggaactt ttccgtggat cgtttgctga tcgatccgcc cttctcctgt aatgatgaca
    29701 tctgcttgtt cgatcttttt ttccaatcca gtgatttggc tgactaaatc gaaaccagaa
    29761 accatcttcg ctcccaacaa aaaaagggct ccaccaatac cgcctgctgc tccacttcct
    29821 ggaatatcat ctaaatccac ctcccagaaa tcttttattt gctgagcaat ctttcctgct
    29881 tgtttatcta agaaagctag cgtcgtatct attcctcctt tttgtcttcc aaattgccaa
    29941 gcagctcctt gttgacctgt atatggattg gtaacatctg cagcgataat caattttact
    30001 ttagaaacaa gaacgtttct tcgatcaaaa tgaagttttt tagtagataa taaaggattt
    30061 ccttctgtgt acccttctaa agaagcaccc aaggattgga gtaatcctag accaccatcg
    30121 ctacacccgc ttcctcctaa agtgacaata atttgttcta cttcttgaag cagtgcatct
    30181 ttaataagtt ctcctaaggc ataactggaa gcaagtcgga tagtttgatc cgttggttta
    30241 tcaagtagat ctaaccccaa tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc
    30301 caagttgtca ataaatagct gatttttcgc tgacggaata agagatccat cccatcaatg
    30361 gtacgccatg ttcccctgag cgcgtaatag attgcttctg cagtattttc tccgccatct
    30421 gcaatcggca cattgatttt tcttccttta cgatcttccc agccatttag aactgcctga
    30481 tttagttcaa atgaagaagc acttccttta aatgaatcaa ttgccgccaa aatatccatc
    30541 tgtatccctc actttcttct taagtaagac actttttttc atttttttca aacaaaacaa
    30601 tgctattttc ttccattttt ctatatacta aaagggaact cattgaataa aggagagaaa
    30661 aaacatgaaa atcgcaatag ctggggccgg agcaatgggc agccgaatcg gattgatgct
    30721 acatcaaagc ggaaatgagg tcctcttgat cgatcgttgg cctgctcaca ttgaagccat
    30781 tcgtacaaat gggttgattg ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat
    30841 ctattcacca gaagaaatca tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac
    30901 aaaagccaat caattagatg atatgttttg ttcgatccaa tccattatta ctgacaatac
    30961 ttatgttttg tgtttactaa atggattagg tcatgaagat gtactggaaa aatatgtgcc
    31021 aaagaaaaat atcttatttg ggatcacgat gtggacagct ggtctagctg gtcccggaaa
    31081 agtcacattg ttaggtgatg gagaaatcga actagagaac ttggaaccag aaggcgaagc
    31141 atttacaaaa aaagttgtag aagtctttca agaagcaaat ttaaatccga tctatagcca
    31201 taacgttcgt tattccattt ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg
    31261 cacgattttg gattgtaata ttgctgaact aggcgcgcaa aaagcggcag agagcatggt
    31321 tcgaacaatc gtatctgaat ttgcttctat cgcagcaaaa gaaggaatca tactagatca
    31381 agaagaagtg taccaacata ttgcttctac ttatgatcct gacaacatcg ggttgcatta
    31441 cccatctatg taccaagacc tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa
    31501 tggtgccatt tggcgaaaag gacaaaaata tgatattgct acaccatatt gtgccttttt
    31561 gacccaatta gtccatgcaa aagaaggaat ccttggcgca gaataaaagg ctctctgatg
    31621 agaatcagct gaatcagcag ctggttctct ttttttaagc ccaataaaag atcaaaaaga
    31681 aaacacaact ctcatgatgc cataagaatt gtgcttgttt gtttcagtac ataatctctt
    31741 caaaatgtag gttttttatt tgttttcaac agaggagagg tcatattcgt attgctcata
    31801 aatagtggaa tcgttagtga taaatcgttt tgcccaattt tctgcatcat aaaattccaa
    31861 tagctctgat aggattggct taaatggtgt atgcagaaat tccggtaatt catctaaatc
    31921 acgttctcga agcaaaaaaa tattttcagg acgataaaaa tgataatgag caatcgcttg
    31981 attatccgtc actagtttct tttgaaaata catggattca aatgtccgca aagtcactcc
    32041 ttgctgaccc tcccgcaaca gttccagaat cccgcgcgat tcccctgttt ttttcaggta
    32101 ctcttcataa gtaagatatc caggttgttc attccctcga tgtggaagaa tgaaatagtt
    32161 ggtcgtaaca tctcttttta taaattcctg cttatagctt tctgctcgtt cttttctccc
    32221 attattcgtt gcaccaaaaa atagatccgt ctttggttcg ctagaagaaa aattcatatg
    32281 tttcgaataa aatgaactat tatgtttcag cccaagttct tttgcttcca aaaaatcaaa
    32341 atgataaatc tcatcgattg ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc
    32401 tgctttgaag tgattccaaa aaaataacac aatttttgca ttggtaaatg ggcgcacgcg
    32461 tcgagccatc tccatatcaa atccataatc taaaaaaacg atcagctgat aatttgccaa
    32521 ctgttgtcta gctgatttaa tgaattccga ctgtagtaaa tcttttccgg accaaacatc
    32581 taaagagcgg atggaaggca aaaaataaag tgatttattt tttgcaacta gcagtacgtc
    32641 atttttgttc atcttttccg cctcactttc tctgtcactt tttcctatga tagcctgttt
    32701 aaatagtaaa aagccagtaa aacgtttcct atatttgtat gaaaatttaa catttgtatg
    32761 aaaatatgat gaaataaatc attcttctat taaaattcgc ctaaaagttt tctgaatagt
    32821 tgtaattgtc tgaaaatttg ttatcattag tcattgttaa atattgctat ttattcttaa
    32881 tataaatttt gattgtgaat ttaaaagaaa gaggtcattt atggaagata aaaaattgaa
    32941 aaaacaactt agctccagac atatcaccat gttagcttta ggtggagcaa tcggtgcagg
    33001 tctatttaaa ggaagtggcg aagcgattgg gatcgccggc ccttctgtat tgattgcttt
    33061 tttgattggt gggctgattt tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag
    33121 cggtggcgac acgcatcacg gattatctgg tttgatacgc ccttatctag gtgctcattc
    33181 tgctgatttt actgattggg tctactggtc tatgtggatt atcaatatca tcgctgaagc
    33241 tgttgcagct gcttcctttt tgcagttgtg gttcccgcac attccagcct gggtatttgt
    33301 ttttatgttg gctgttttaa ctacgatcat caatctttat tcggtacggt tattcgcaga
    33361 aacagaatat tggctggctt ttgctaaaat cagtgtcatt atcttactga tcatttttgc
    33421 tacttatctt gtcggtcagc aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt
    33481 gactgaccat ggtgggttta ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt
    33541 agttatctat tcttacggcg gttctgaact gattgctatc acggtaagcg aagcagatga
    33601 tcctaaacgt gcgattccta aagcaatcaa aggtgtcatg ggaagaatca tttcctttta
    33661 catcattcct ttgtttttgc ttttgattct ttttccttgg aatactttag caggcacgaa
    33721 tgtcagtcct tttgtgatgg tatttgaaaa aatgaatatc ccatttgcag cggatatcgt
    33781 caattttgtg attgtccttg ctttgttctc atctatcaat tcgggtgtgt atgcatcttc
    33841 aagaattttg ttcttccgtt tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt
    33901 aaacaagcat caagtaccgc aacgtgctgt tttattttgt accagtgttc tttatcttgg
    33961 tgtagctctt tcttattttg tcggtgacaa attatttggc tatctggcag gttccctttc
    34021 gtatacagta ttattgatct ggatcattat cagtgcggca tcctttgtac tggcactaag
    34081 aaaaggaaca ttatgggata aaggaatcag cttttttgca ttagctgtct taggattgat
    34141 tttccttggt atcctgttca cgaactccat tggggtaact gtattgactg gcctgcttta
    34201 cctgttcatt tatttcagtt atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg
    34261 aaatcgatag aaaattttat tttatttatg tggaatagat ggagctattg aagaaaggag
    34321 ttgtgacaaa agtcttgttg caactccttt ttccgaataa acagtctcat gacctcttgg
    34381 taaacggtgt tcaaaaaccc tctgtgcaaa ataattgatt gttctccatg ctcatgacat
    34441 gatagaagaa
//
LOCUS       DACDRK010000020        34387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDRK010000020 DACDRK010000000
VERSION     DACDRK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.020"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            190..420
                     /locus_tag="IWJ77_001101"
     CDS             190..420
                     /locus_tag="IWJ77_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9452736.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            517..1818
                     /gene="murA"
                     /locus_tag="IWJ77_001102"
     CDS             517..1818
                     /gene="murA"
                     /locus_tag="IWJ77_001102"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9452737.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1831..2007
                     /locus_tag="IWJ77_001103"
     CDS             1831..2007
                     /locus_tag="IWJ77_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9452738.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2142..2438
                     /gene="yidD"
                     /locus_tag="IWJ77_001104"
     CDS             2142..2438
                     /gene="yidD"
                     /locus_tag="IWJ77_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9452739.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2623..3090
                     /locus_tag="IWJ77_001105"
     CDS             2623..3090
                     /locus_tag="IWJ77_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9452740.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3175..4059
                     /locus_tag="IWJ77_001106"
     CDS             3175..4059
                     /locus_tag="IWJ77_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9452741.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4176..4409
                     /locus_tag="IWJ77_001107"
     CDS             4176..4409
                     /locus_tag="IWJ77_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9452742.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4497..4577
                     /locus_tag="IWJ77_001108"
     tRNA            4497..4577
                     /locus_tag="IWJ77_001108"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4531..4533,aa:Tyr,seq:gta)
     gene            4584..4655
                     /locus_tag="IWJ77_001109"
     tRNA            4584..4655
                     /locus_tag="IWJ77_001109"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4616..4618,aa:Gln,seq:ttg)
     gene            5170..5877
                     /locus_tag="IWJ77_001110"
     CDS             5170..5877
                     /locus_tag="IWJ77_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9452743.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5936..6580)
                     /locus_tag="IWJ77_001111"
     CDS             complement(5936..6580)
                     /locus_tag="IWJ77_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9452744.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6706..6993
                     /locus_tag="IWJ77_001112"
     CDS             6706..6993
                     /locus_tag="IWJ77_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452745.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7101..7421
                     /locus_tag="IWJ77_001113"
     CDS             7101..7421
                     /locus_tag="IWJ77_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9452746.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7502..8815
                     /locus_tag="IWJ77_001114"
     CDS             7502..8815
                     /locus_tag="IWJ77_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9452747.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8812..9531
                     /locus_tag="IWJ77_001115"
     CDS             8812..9531
                     /locus_tag="IWJ77_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9452748.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9659..10210
                     /gene="raiA"
                     /locus_tag="IWJ77_001116"
     CDS             9659..10210
                     /gene="raiA"
                     /locus_tag="IWJ77_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9452749.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10432..12966
                     /gene="secA"
                     /locus_tag="IWJ77_001117"
     CDS             10432..12966
                     /gene="secA"
                     /locus_tag="IWJ77_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9452750.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13092..14193
                     /gene="prfB"
                     /locus_tag="IWJ77_001118"
     CDS             join(13092..13154,13156..14193)
                     /gene="prfB"
                     /locus_tag="IWJ77_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9452751.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14298..14984
                     /gene="ftsE"
                     /locus_tag="IWJ77_001119"
     CDS             14298..14984
                     /gene="ftsE"
                     /locus_tag="IWJ77_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9452752.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14968..15861
                     /locus_tag="IWJ77_001120"
     CDS             14968..15861
                     /locus_tag="IWJ77_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452753.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16013..17461
                     /gene="cls"
                     /locus_tag="IWJ77_001121"
     CDS             16013..17461
                     /gene="cls"
                     /locus_tag="IWJ77_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9452754.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17798..18655
                     /locus_tag="IWJ77_001122"
     CDS             17798..18655
                     /locus_tag="IWJ77_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9452755.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18770..19690
                     /gene="pstC"
                     /locus_tag="IWJ77_001123"
     CDS             18770..19690
                     /gene="pstC"
                     /locus_tag="IWJ77_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9452756.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19690..20574
                     /gene="pstA"
                     /locus_tag="IWJ77_001124"
     CDS             19690..20574
                     /gene="pstA"
                     /locus_tag="IWJ77_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9452757.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20589..21395
                     /gene="pstB"
                     /locus_tag="IWJ77_001125"
     CDS             20589..21395
                     /gene="pstB"
                     /locus_tag="IWJ77_001125"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9452758.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21411..22169
                     /locus_tag="IWJ77_001126"
     CDS             21411..22169
                     /locus_tag="IWJ77_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9452759.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22182..22859
                     /gene="phoU"
                     /locus_tag="IWJ77_001127"
     CDS             22182..22859
                     /gene="phoU"
                     /locus_tag="IWJ77_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9452760.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23096..24127
                     /locus_tag="IWJ77_001128"
     CDS             23096..24127
                     /locus_tag="IWJ77_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9452761.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24124..24891
                     /locus_tag="IWJ77_001129"
     CDS             24124..24891
                     /locus_tag="IWJ77_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9452762.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24991..26559
                     /gene="liaX"
                     /locus_tag="IWJ77_001130"
     CDS             24991..26559
                     /gene="liaX"
                     /locus_tag="IWJ77_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9452763.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26859..27176
                     /locus_tag="IWJ77_001131"
     CDS             26859..27176
                     /locus_tag="IWJ77_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9452764.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27176..27526
                     /locus_tag="IWJ77_001132"
     CDS             27176..27526
                     /locus_tag="IWJ77_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9452765.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27660..28979
                     /locus_tag="IWJ77_001133"
     CDS             27660..28979
                     /locus_tag="IWJ77_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP9452766.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            28951..29148
                     /locus_tag="IWJ77_001134"
     CDS             28951..29148
                     /locus_tag="IWJ77_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452767.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            29334..30272
                     /locus_tag="IWJ77_001135"
     CDS             29334..30272
                     /locus_tag="IWJ77_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9452768.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            30287..31117
                     /locus_tag="IWJ77_001136"
     CDS             30287..31117
                     /locus_tag="IWJ77_001136"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9452769.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            31161..32186
                     /locus_tag="IWJ77_001137"
     CDS             31161..32186
                     /locus_tag="IWJ77_001137"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9452770.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            32201..33139
                     /gene="galU"
                     /locus_tag="IWJ77_001138"
     CDS             32201..33139
                     /gene="galU"
                     /locus_tag="IWJ77_001138"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9452771.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            33264..33704
                     /locus_tag="IWJ77_001139"
     CDS             33264..33704
                     /locus_tag="IWJ77_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9452772.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            33707..34309
                     /locus_tag="IWJ77_001140"
     CDS             33707..34309
                     /locus_tag="IWJ77_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9452773.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT        11456 a         5802 c         7464 g         9665 t
ORIGIN      
        1 tatttggaat aagggactgc gacaagactt ttgtctcagt cccttttttt gcatattttt
       61 agcatttatt tcctaaaaaa tgtaagggga ttcctatatt tttaagtata cttgtttttc
      121 cttttataca ttttatggta ttatcatttt attagttatt ataaaacaat cgttagaaag
      181 gacgtcatta tgcaagtata cggaattgat gcggtgcttc gaatcgtgag tcatttggcg
      241 tttatctact tagccttctg gtctttacgt tcgttacgca ttgaaacttt tttcagacca
      301 atgcataata cgcaaattag aatggcaatc gtactttttt caattttttt agggtacact
      361 gcaagtaatt ttttcttaga agttatcgct ttatgccgaa atcttttcgt tagcctgtaa
      421 attttaaaaa aaggtactat tttaaaagca aggatgttat aatagaaaag attttgagta
      481 agtgtatgct cattggaatt ttttggaggg aacaacatgg aagagatcat cgtaagaggt
      541 ggcaatcaat taaatggaac agtaagaatt gaaggagcta aaaatgctgt tttgcctatt
      601 ttagctgcta gtttgttagc agaagaaggt attacgacat tagacaacgt tccgattctt
      661 tctgatgtct tcactatgaa tcaagtgatt cgtcacttaa acgtagatgt agattttgat
      721 gaacaaaaga atcaagtaac aatcgacgct tcaagacaat tagaaatcga agcgccttat
      781 gagtatgtta gtcaaatgcg tgcttcaatc gttgtgatgg gtccgctact tgccagaaat
      841 ggacatgcaa aagtagctat gcctggtgga tgtgcgattg gtaaacgacc aatcgatttg
      901 catttaaaag gatttcaagc tttaggagca aaaattattc aaaaaaacgg ctatatcgaa
      961 gcaattgcgg atgaattgat cggtaacacg atttatttag atttccctag tgtaggtgct
     1021 actcaaaata tcatgatggc tgccgtaaaa gcaaaaggaa caaccatcat tgaaaacgta
     1081 gctcgcgaac cagaaatcgt tgatttagca aacattttga ataaaatggg cgctcaagtc
     1141 tatggagctg gtactgaaac aatgcgtatt gaaggcgtgg atcatttaca tgcagtcaac
     1201 cactcaatcg tacaagatcg tatcgaagca ggtaccttta tggttgctgc tgcaatgaca
     1261 caaggcaatg tgctgattgc agatgctatc tctgaacata accgcccatt gatttctaaa
     1321 ttgatcgaaa tgggtgcaga aatcattgaa gaagaaggcg gagtccgtgt catcggacca
     1381 aaacatatct tacctacaga tgtaaaaacg atgcctcatc ctggtttccc tacagatatg
     1441 caagctcaaa tgacagctat ccaattagtt gcagaaggta caagtgtagt gactgaaaca
     1501 gtgtttgaaa atcgtttcca acatttagaa gagatgcgcc gtatgaacgc tcatgtcaaa
     1561 atcgacggaa atgtagcaat catggatgga aaccatgaat tgcaaggtgc agaagtatat
     1621 gcaacagact tgcgggcagc agcagcatta gttttagctg gcttgaaagc aaatggaatc
     1681 acacgtgtgc gtaacttgaa ttatctagac cgtggatatt ataatttcca tatcaaacta
     1741 cagcaattag gtgcagacgt tgaacgtgta gacatggatc aaacttctgc tgaaaaaaca
     1801 gcacaaacga ttgcttaagg aagtggctga atgagtaacc aacgatatat cttattaact
     1861 ttgataaaga ttttaatggt gatcttattg cttatccttc tttttgtggc cggaacgatg
     1921 atcggatatg gtgtcatcgg tggcggcaat ccatttaaag tatttcagcc aagtttgtgg
     1981 attcatattc gagacttttt tcattgataa ataagaatca cagtggttct agtcgatatg
     2041 aagtgagcca tgaagcagaa catgataaaa gaaaacagaa gggaacgttg acgttagtcg
     2101 cgatcccttt ttttagtaaa agaaaagaag gaatctaatt gatgaagaag atattgattt
     2161 atttagttag aggatatcag cgctttattt cgcctgcatt tccacctagc tgccgttatt
     2221 acccaacctg ttccaattat atgataaaag caattcaaac ccatggcgcg ctaaaaggga
     2281 cggtaatggg gactgcacgg atcttacgct gtcatccttt tgtcaaaggc ggtatcgatt
     2341 atgtaccatt gacattccga ttaacgcgaa ataagaaaga taaaaaagaa ccagattata
     2401 aaaaggttgt gaaaaatacg ggtagcactg agcgataaag aaacaatttg gaaaatagct
     2461 tcttatattt tgcaaaattg tgagttattg ccgaagtgtt ggattttgaa caccgtcaaa
     2521 aaaaaggttg tgaaaaatac ggctagcact gagcgataaa gaaaaagagg aaatttattg
     2581 tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga agaactaaac
     2641 aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga aaaagttcaa
     2701 gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac aataggtgta
     2761 ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt aaccccaacg
     2821 tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa gctttcacaa
     2881 ctcgcttcca gtcaaactca gatcatcata cgaaccggca gtactagcag tgcggcgtta
     2941 catctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta ttttatccat
     3001 cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt gattttagca
     3061 attcacttac ctaaaaatag ctttaactaa taaaatttca attcaagaaa atgctttcaa
     3121 atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat ttttatggga
     3181 atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga tggcaaagag
     3241 attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa agcgcaatta
     3301 tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg aatcagctct
     3361 cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat tccttatatt
     3421 catggattta atattttccg agatttggaa actctgtttc aacttccagt aacgattgaa
     3481 aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg cgattatcaa
     3541 gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac gaaaggacaa
     3601 atcaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt aaatggcgat
     3661 aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg tgaacgcaaa
     3721 ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga aactggagat
     3781 gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg attattcagt
     3841 attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc tgcaaaagaa
     3901 gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt cgatctgcat
     3961 gatttcgaac cgcagatttt attatgtgaa tacagaaacg atgctaatct tgtaggtgca
     4021 gcagcaaact atctcgctgt acgtcaaggg gagaattaag agctttagag aaggtgcagc
     4081 ttgcataaaa gaagtgaatg aaatgaatag gagattttta cctaaggaat gttttatttg
     4141 ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat aaagatattg
     4201 aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa gtcactcagt
     4261 tatttatagg gaaaaaaatg attggtgaga tcttagccta tggaccaaaa gaatatgaaa
     4321 ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg atagaaacag
     4381 tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc aataaagggt
     4441 tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa cctttcggag
     4501 gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt tcagtggttc
     4561 gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag ggactttgac
     4621 tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta agctctcgtt
     4681 agttcgagag ctcttttttt tgctttgcta ccttgttcct tgaagattct gctgatttaa
     4741 gatagagtta agttaactac aagaattgga gggatccaca tgaaatggaa agaaaaagta
     4801 gacgaagcag ctgaaaagtt atatgatttg gtcaaatcag aaaaatacaa tattgaagtg
     4861 aatattccga aaaaaggcgg aaaagcggtc cgggtaaaat caaaacgccc aacgaatcat
     4921 acaaaaaaat ggatggctaa aaaccgctaa taataacaaa agatccataa gtcggcattt
     4981 tcatcgattt tagggtcttt tgttattttc taggttggac atatctaaaa aattgtttct
     5041 gacaatagga gtaggaagaa cgatgtgtgt ctaaatcctc tgttgatgga tagaaacctt
     5101 tatctttttc agttgtgaat caggtatata ccagttataa tagaagaaac gaaaggacgg
     5161 gtgtacgtta tgtcgaatca attacctgta tatatacaga ttcatgacca attaaaaaat
     5221 gaaatcgaac aaggtatatg gaaagtcgga gaccgtcttc cttcagaaag agaattagca
     5281 gtgaaattca atgtcagccg tatgacgttg agacaagcaa ttcaaaactt ggcagatgaa
     5341 ggaatcttag aaagaaagat cggttcgggt acatatgttg cacgagaaaa agtacaagaa
     5401 aaaatgtctg gtgcaacaag ttttacagaa attatggaat cacaaaatcg agtaccgtct
     5461 agtcggacaa tctcttattt cttgacttcg ccaagctcaa gtgaaatgga aaaactaaac
     5521 ttgagtaaag atgacacgat cttgcgaatg gaacgtattc gtttcgcaga tgatgtccct
     5581 atttgttttg aagttgccag tgtgccacaa aagctgatcg atggctacag caaagcagag
     5641 attactgaat cgttgtatcg cacgctagaa gaaaaaggcg ggaaaaagat cggtccagcg
     5701 aatcaaacga ttactgctgt gttggcttct gagaaaattg ctgactattt agaggtaaaa
     5761 cgaggagatg cagttcttcg tttgcgccag atttcttatt ttgaagacgg tacgccgttt
     5821 gagtatgtgc gtacgcaata cgtaggaaat cgttttgagt tttatttgga aaaataaaaa
     5881 acaggtgatc cctgattccg tattagaaat aaaagagaat cagaatcacc tgtttttata
     5941 tatgatcagt taccagttgc tcgtgataag aaacaggacc ctcttcaaat tggacttgtc
     6001 cgttcagcag ctcaatgatg gattctttaa agtggtcagc acttttttca tctacagcac
     6061 agatgacgct aacttgatca gtatattgga tttcttttat aaaaaaatgt tggagttcaa
     6121 gataattctg taattttcct agttgcggat acgtgatcat cacagagatc tcctgttgca
     6181 aggtaccttc tacgattcca atgtcagaca atgcttgaga gaccgcatga gtgtatgctc
     6241 gtattaatcc gcctgtgccg agtttcgttc ctccaaaata tcgagtgact actgcagcta
     6301 cattgatcag ttgatttttt ttgaggactt ccaacatcgg tatgcccgcc gttccacttg
     6361 gttctccatc atcgctgctt cgctggattt catttttttc tccaatgatg aaggcgctgc
     6421 agttatggtt cgctttccaa tgttcttttt ttatttcttg aatgaaactt tttgcttcgt
     6481 cctctgattc tattcgtcta agagaacaga taaagcgtga ttttttgatc tcgatctcac
     6541 tttgtccatt ttcttttatt gtacgatagc gctcgatcat cttgtcacct cttttttcat
     6601 tttctttatt cagtataaat aaagttctag gttgtttcaa cactgtaacg gttgcgcaat
     6661 tgctgtagta tgcgataata aagacaaagg caggtggatc tggggatgca ggaacgaatg
     6721 aaaaagtacg atgcaattac gcattatttg aagaacaatg gcggctctca agtaacgttg
     6781 acctttaccc aatttgacga actgctgttt cccagtaacg gacttcccaa aacagcaagg
     6841 gaaagtacag attggtgggc aaatgattat aaacaccctg aaaaaggtgc ctatggctgg
     6901 ataaatgcag gctatgaagt agttgtcatc aatttggaca aagaatatgt cgtgttcaat
     6961 aaattagtga aatctagttg gctatttgat taaataaacc gcgtttagaa aacgttgaca
     7021 aatttctttc tttttatata atagagtaac agacgcgaag aaaacgtttg cgtagtttgt
     7081 tttaaaaagg agagaaaagt atggctaaaa aaacaatcat gcttgtatgt tcggctggta
     7141 tgagcacaag tttactagta acaaaaatgc aaaaagctgc tgaagcaaaa ggaatcgact
     7201 ctgatatttt cgctgtgtcc gcttctgacg cagataacaa tcttgcaaat aaagacgtgg
     7261 atgtgttgtt attaggccca caagttcgct ttatgaaagc tgattttgag aaacgtctgg
     7321 aaccaaaagg cattccttta gatgtcatca atatggctga ttatggtatg atgaatggcg
     7381 aaaaagtgtt agatcaagca atcacgctca taggtgaata aatagaacag tataactatc
     7441 gaacaagcga tccaaaaaat agatcgcttg ttttttcgta tattttttta gggcgaataa
     7501 tatgaatcat ttttggggta gaaggcttct gacgagagat attaaagcaa tagactgtga
     7561 ggaggtaaat ttaccaaatt atgagaagat cgcagcagtg gaacgtttga gcaactggat
     7621 tctttgtcat cgctgtggaa tgagaacgtc cgtcattgat ggacagcttg cagattacgg
     7681 ttatttttgt acacattgtc tatatttagg gcgatgcgac actcaacaag atttatattt
     7741 gtttgatcag cctgaagcaa aacctcgaga agtcgtcttt ttttggacag gacagcttac
     7801 tgaaaaacaa acggaaatct caaagagaat actttgccat tcagaaaaaa gacatcatct
     7861 tatttgggct gtaacaggtg cagggaaaac agagatgctt tatcctattt tagtggaaac
     7921 tttaaaagct ggaggaagag tagcgatttg tacaccgcga gtcgatgttt gtaatgaatt
     7981 gtttttacgc tatcgttatg tttttccaga agaaaaaatc acgttgctcc atgggaatac
     8041 agcaacggct tataccttta cttcttttgt tatctgtacg attcatcagc tataccgttt
     8101 ttatcggtcc tttgatttaa tagtgatcga tgagatcgat gcgtttcctt ataacgggga
     8161 tgcaggatta gctcatgctg tgcaaacagc gattaaacca gacggacgat tcatctactt
     8221 aagtgccaca cctgataaaa agttactaaa ggagatcaag cagacgtttg acatccacaa
     8281 acttgcttta cgctttcatc ggcgtttgct tcctgaaccc gtcttggttt tcatgaataa
     8341 ttggcgacaa aaatgcaaga acaagaagaa gataaaatct ttggtgcgga tcattcatca
     8401 attaattaaa gaaaaccata tccttttatt ttgtccaagt atcacgatga tgcatcgttt
     8461 gaaagccaca cttcaagagg ctcttccgag atatgagatc acagatgttt cttcaagaga
     8521 caatcagcgg gcggaaaaag tccagaaaat gcgagaagca aaatatgaca tactgttaac
     8581 cacgatgatc cttgaacgtg gagtcacatt tgaaagaata tccgttatcg ttttaggagc
     8641 ggatcatccg gtttttacta aatcttcttt ggtacaaatc gcaggaagag ccgatcgtaa
     8701 agggccttat acgaacagtc aagtatattt tttctatgaa gaaaaaactg cagctattcg
     8761 aaaagcttgt caggaaatca aagaaatgaa tgaagaaggg aagaagatac tgtgaggtgc
     8821 agttgctgca gtaaagatat cgtccaaaat ttaacattca gcgagattat tggtgtttcg
     8881 aaatacagat tacctcagac ttgttcagtt tgtcgcgcac aatttcattt attggaaaca
     8941 agaggctgtt ctggctgtca aaaaccaatc catttcaatc tagagagtac gaaaaagaaa
     9001 gtaaattcta tccccgttta ctgcagtgac tgtcaagaat ggaagaagaa atatccgacc
     9061 tatgattttc agcaccgtgc cttttttgtg tacgatgaaa atatgaaggc ctggctcaat
     9121 caatacaagt ttatgggaga tatccgacta gcaggcacct ttgcatataa atggacgcag
     9181 ataaaaaaag aatacccgac atacatctat tgtccaatac ctttatctga aaaaaggcga
     9241 acagaaagag gattcaatca agtaagcgag atgttgaaag cagcaagaat tccttttcag
     9301 atgctactca aaagagagaa gcatctgctg gctcaagcaa aaaacacgcg aaaggaaaga
     9361 ttggagatgc ctcaaccttt tgaacttgca gtcacctttt ctaagataca aaataaaaaa
     9421 atattgatta ttgatgatgt ctatacaaca ggacagactc ttttccacgc agcagattgt
     9481 ttgcagatcg cacagccgaa aatcatccga actatttctt tggcgagatg aattagatgt
     9541 ggaaatttga tagcgattta atttgcaaat aacctagagt tcgatataat agaattaaga
     9601 agagggaaag agtggtcctt ctccttcttg attgtggtag acgaaagggg taatttttat
     9661 gtttagatat aatgtacgcg gcgaaaatat cgaagtaact gaagcgattc gcgactacgt
     9721 tgagaaaaaa gtaggcaaac tagaacgcta tttcagtgac gcacccgatg caacggcgca
     9781 tgtgaactta aaagtttata cagaaaaaac agcaaaagtg gaagtaacga tcccattacc
     9841 ttatcttgta ttacgtgccg aagaaacttc tccagattta tacgcaagta tcgatttggt
     9901 tgttgacaaa ttggaacgcc aaatccgcaa attcaaaaca aaaattaatc gtaaatcccg
     9961 tgaaacgggc atgaatactg caaatgctgc tgctttattc aatgaagagg acgaaaacga
    10021 aaatgattct gaattagata tcgtacgtac gaaacgcctt tcattgaaac caatggatag
    10081 tgaagaagca gtcttgcaaa tgaacatgct gggacataac ttctttattt ttgaagatgc
    10141 agaaacaaat ggtacaagta tcgtttaccg ccgtaaagat ggaaaatacg gactgattga
    10201 aacagactga tcagccttac ttaaataaca acgaaaactt gcagcagcta ctgacagctg
    10261 ccgcaagttt ttttgagttt cctcctcttt tagatgaaat atgaataaat tatgaagttc
    10321 ttactttaat acgcttaaga tatttcaatt atctataact aatgataaaa tgaaaagtaa
    10381 agtctacata gtctggataa tacacagaag gaaaaggaga ttacacaacg catggccaat
    10441 ttgattcgtt cacttatcga aaacgataag aaagaactaa aacgtttaga tagcattgca
    10501 aaaaaagttg aagcatacgc tgaccaaatg gcagcattaa ctgatgaaca attacaggca
    10561 aaaactgctg aattcaaggg cagataccaa aaaggagaaa cattagatca attacttccg
    10621 gaagcttttg cagtcgtccg agaagcagca aaacgtgtat taggtttata tccataccac
    10681 gtacaattga tgggtggaat cgtgcttcac gatggaaata tccctgaaat gcgtactggt
    10741 gaaggtaaaa ccttgaccgc aacgatgcca gtatatctaa atgctctgac tggcgaaggg
    10801 gtacacgtag ttaccgtcaa tgaatattta gcaacccgtg actcgacgga aatgggcgaa
    10861 ctttataatt tcttaggatt aacagtgggg ttgaatatca actcaaaatc ttcggatgaa
    10921 aaacgcgagg cttataattg tgatatcact tatagtacga ataatgaatt aggatttgac
    10981 tacttgagag acaatatggt cgtttaccgc catcaaatgg tacaacgtcc gctgaattat
    11041 gccattgtcg atgaagttga ctcaatcttg attgatgaag cgagaacgcc gctgatcatt
    11101 tcagggcaag cagaaaaatc cacagcttta tatacaagag cagacaactt cgtgaaacgt
    11161 ttgaaagaag aagaagatta taaaatcgat gtacaatcaa aaacaatcag tttaaccgaa
    11221 gcagggatcg aaaaagcaga agaaacattc ggtttggata acttatacga cattgaaaat
    11281 actgcattga cccatcactt ggatcaagca ttgagagcca actatatcat gcttttagat
    11341 atcgattatg ttgtccaaga cggaaaagta ctgattgttg accagttcac tggtcggatc
    11401 atggatggac gccgttattc agatggactt caccaagcaa tcgaagctaa agaaggcgta
    11461 gagatcgaag acgaaacgaa aaccatggcg acgatcactt tccagaacta tttccgtatg
    11521 tacaagaaac ttgcgggaat gactggtaca gctaaaacag aagaagaaga attcagagaa
    11581 atttataaca tccaagtata ccagattccg acaaatcgtc cggttatccg tgatgatcgt
    11641 ccagatttgc tttacccaac attacaaagc aagttccacg ctgtcgtgca agatatcaaa
    11701 gatcgctacc gcaaaggtca acctgtgtta gtcggaactg ttgcggttga aacatctgag
    11761 ttattatcag atatgctgaa tcaagaacgt attcctcatg aagtgctgaa tgcgaaaaat
    11821 cactttaaag aagcagagat catcatgaat gctggtcaaa aaggtgctgt gacgattgct
    11881 accaacatgg ctggtcgggg aacagatatc aagttaggac ttggggtaag agaacttggt
    11941 ggtctagcag ttatcgggac agaacgacat gaatctcgtc gtatcgataa ccagcttcga
    12001 ggacgtgcag gacgtcaagg agaccctggt atgtcacagt tctacttgtc attggaagat
    12061 gatttgatga aacgattcgg ttcagaacga atcaaagctt tcttagatcg gatgaagatc
    12121 ggtgaggaag atgctgttat ccaaagcaag atgctgacga aacaagtaga atctgcacaa
    12181 aaacgtgtag aaggaaataa ctatgacaca cgtaaaaacg tcttacaata cgatgatgtg
    12241 atgcgtgaac aacgtgaagt aatctataaa cagcgacaag aagtaatcat ggaagaaaaa
    12301 agcttatcca agccactaat gaacatggtc aaacggacaa tcagtcgtgt agtagatgcg
    12361 catacacaat tagagaaaga aaattggaat ttggaaagta tcatcgattt tgccggcagt
    12421 actcttgtcc atgaagatag tattactctt gcagatatcg aaggaaaaac agctcaggag
    12481 atcaaagagt atctaaacga acgagcacaa gaagtattta ataccaaagc ggctcaacta
    12541 aatggtccag agcaactgtt ggaatttgaa aaagtcgtga tcttgcgtgt tgtggactct
    12601 aaatggacag accatattga cgcaatggat caattacgtc agtcaatcgg cttacgtgct
    12661 tatggacaga acaatccatt agtcgaatat caaacagaag gctataagat gtttgaagat
    12721 atggtaggcg ccatcgagta tgaagtcact cgaatcttta tgaaagctga gatccgtcaa
    12781 aacgtacagc gtgaacaggt ttctcaagga acagcttctc actcagaaga cggagatgcc
    12841 aaagaaaata gtaatactag cgcgaaaaaa cagccaatcc atgtgaacaa ggtcggaaga
    12901 aatgatcctt gtccttgtgg aagcgggaaa aaatataaga actgtcatgg aaaaaatgca
    12961 aactaatttc tgacagcagg attggtgaga caaattcttt ttttaagaat tcgtctcacc
    13021 ttctttctgt tatttgctga ttctgttaga ctagatataa tgaaaaaagt atagtgaaag
    13081 aaggaaaaaa catggaaata tccgaaattc gaaatctcct gacagaaatg caggaaaagg
    13141 tcacaagttt caggaggtct ctttgactta gatcagctgg aagaagacat cgcggaagca
    13201 gaagcaaaaa tggctgaacc tggtttttgg gatgacagcg aagccgcaca aaaagtgatc
    13261 agtgaaaata acgctaataa agaaacctat gaccaattcc attctcttgc cgaagaacta
    13321 gaagaaatcg aattgatggt ggagatgctg caagaagaac ctgatgcagc aatgcaagaa
    13381 gaagcagaaa cacgtatcaa tgcattagat gaaaaaatga agacgtatga attatctatg
    13441 ctgttagacg gaccttatga tcgaaacaat gcgatcgtcg aattgcatcc aggggctggc
    13501 ggaacggagt cccaagactg gggcagcatg cttttacgta tgtatacacg ttgggcagag
    13561 cagcatggct atcaagtaga aacactagat tatcaagcag gtgatgaagc agggatcaag
    13621 agtgtgaccc tgctgattaa aggacataac gcttttggtt atctaaaatc agaaaaaggt
    13681 gttcatcgcc ttgtacggat ttcgcctttt gactcagcga aacgacgtca tacttctttt
    13741 tgttcggtag atgtgatgcc agagcttgat gaaacgatcg atatagaaat caatccagat
    13801 gatttgaaaa tcgatacgta ccgagcaagt ggtgcaggtg gtcagcacat caataaaaca
    13861 gaatctgctg tacggatcac tcacttgcct acaggaacag tagtagctag tcaggcacaa
    13921 cgctcacaat tgaaaaaccg tgagcaagcg atggggatgt taaaagctaa gctttatcaa
    13981 ttagaagtgg aaaagaaaga acaagaagca gctgcacttc gaggagaaca aaaagaaatc
    14041 ggctggggtt cacagatccg ttcttatgtt ttccatccct attctatggt aaaagaccat
    14101 cggacaaacg tagagacagg aaatgtccaa gcagttatgg acggtgacct tgatttattt
    14161 attgacggct atttaaaaat gcatctacaa taagaaccca ttgttaaatt gaaataaaat
    14221 tgtaagattt tgcaagcatg ttgaaacatt gccatgctat aatagctaaa gactgaaaaa
    14281 gatatggaga gaacgccatg attgaaatga aggatgtaat gaagaagtac tcaaatggta
    14341 cgaccgctat ccgcaatctc tcgattgtca tcgaacaagg agagtttgtc tatgtggttg
    14401 gtccgtctgg agccggtaaa tctacattca tcaaattgat gtatcgagaa gaaaaagctt
    14461 caaaaggaac tttgaacgtc gcaggtcatg atctaatagc aatcaaaaac cgcgaagttc
    14521 cttatctacg cagagaaatc ggcgtggtgt tccaagacta taaattatta ccaagaaaaa
    14581 cagtatatga aaatgttgcg tacgctatgc aagttatcgg cagaaaacct cgagatatca
    14641 aaaaacgagt aatggaagtg ctggaccttg ttggtttgaa acacaaagtt cgagtctttc
    14701 ctagcgaatt atccggtggg gaacagcaac gtgtctctat tgctcgggca atcgtgaata
    14761 cgcctaaagt acttatcgcg gatgaaccga caggaaatct ggatccagaa aactcatggg
    14821 aaatcatgaa attattagat cggatcaatg cccaaggaac tactgtcgtg atggctacac
    14881 ataacagtac gatcgtaaat acgattcggc accgagtaat tgctatcgaa aacggccgca
    14941 ttattcgaga tcaagcggaa ggggaatatg gatacgatga ttagaacctt tttccgccat
    15001 ttattagaaa gtatcaagag tttgaaacgt aacggctgga tgacagttgc ttcagtcagt
    15061 gcggtaacga tcacgttgac tttagtcggt gtattcatgg cagttatcat gaatgcaact
    15121 aaattagctc aggatataga aggaaacgtt gatgtatctg tattcgtaga tatcggtaca
    15181 aaagacgaag agatgaaaaa gctagaaaaa gaattgaatg acttagatca tgttaaaaaa
    15241 gttgaatttt caagtaaaga acaggaattg aaaaaaatcc aaaacgaaat gggtgattcc
    15301 tggaaattgt ttgaaggtga caataacccg ttatatgatg tgtatgtagt aagtgctact
    15361 gatccttctt atacaaaaca agtagcagaa gaagcagcag atttgaagaa tgtttctcgt
    15421 tctgattacg gtggagcttc ctcagatcgg attttccaaa tcgctggtgc agttcggaca
    15481 tggggattag ttgcagcggc attgctgctg ttcgtagcga tgtttctcat ttcgaacacg
    15541 attcggatca cgattttgtc tagacagcgt gaaattcaaa tcatgcgttt agtcggtgca
    15601 aaaaatggat atattcgctg gccgtttttc cttgaaggtg gctggatcgg tctgatcggt
    15661 gcgattcttc cgattttgat catcacattt ggttatgcat ggtttttcca attgatcaat
    15721 ccatcattat tacgttccca ttattcattg attcatccac aaaatatcgt ttggaaaatc
    15781 aatcttttaa tggtcggcat tggtgtgatc attggttcac ttggctcgat catttctatg
    15841 agacgtttct tgaaattcta ataccacaat ataaataaaa tgagaaagtt ggacagtttc
    15901 gttccagctt tcttttttta ttggctagga gaatagaagg aagcggcttt tttcccttta
    15961 ctttaaaaaa caacttgggt attcttaagt tggtagagga agaggtgaag ccgtgaaaat
    16021 tatcgttgac aattttttta cgatcctttt gattatgaat attttgcttt cctttatcat
    16081 cgtttttcga gaaagaaagg aaactgctca aacatgggca tggctgctcg ttttgatgtt
    16141 cattccagtt gtaggattta ttttatatat ttttttagga agagggattt ccaaagagaa
    16201 aatctttgat ttgaagatcc aagggaaaat cggtaagaac ctggaaattg aagaagacaa
    16261 acaggcattg atgcgagggc tttatcctca tccgcctact ggacaagtag atgtgaaaca
    16321 attgatctat atgctcacgg tatttgaaag tacgctctat acgactaaaa atgaaatcac
    16381 tttatttaca gatggtcgtg aaaaattcga tgcgctgatt gaagatatca agcaagcaga
    16441 agaccatatc cacttccagt attatattta tcgcagtgat gcattaggtg aagaagttcg
    16501 agatgcattg acagaggcgg caagacgagg ggtaaaagtc agagtccttc ttgatgcatg
    16561 gggttccaca caagtttctt ccagcttttt tcagaatttg aaaaaagcag gcggagaaat
    16621 cgcatttttc tttcctctat ttgttccgta tattaatcct cggatcaatt atcgcaatca
    16681 tcgtaaaatc gtagttattg atgggaaaat cggatataca ggtgggttca atgttggaaa
    16741 tgaatatcta ggattggtaa agaaatttgg ttattggcga gacaatcatt tgcgtattta
    16801 cggagaagcg gtgtatatct tgcaaaaccg ttttttgatg gactggaatt ctcagcatac
    16861 aaaagaagta ggctattctc ccaaattttt tccgtccatt cactctactg gtgatatagc
    16921 tgttcaaatc gtgacaagcg gaccagatac cgaacatgaa caaatcaaaa tgacttattt
    16981 gaaaatgatt tcgttagcca aacgggagat attgatccaa acgccttatt acataccgga
    17041 tggatcgatt catgaagcac tgaagttggc tcttctttca ggcgtaaaag ttcatatcca
    17101 aatacctaac aaaccagatc atcttctcgt ttactgggca acgtattctt tcgctgcaga
    17161 gctgatcgag tatggagcaa ggatcgaaac atatgagaat ggatttattc atgcaaagac
    17221 gatgattatc gatggtggca ttgtttcagt tggctcagca aatatcgatg ttcgttcatt
    17281 tcgcttggac tttgaggtca atacactgat ttatgatgaa cgaatggctt cacgtgtaag
    17341 aaaagcattt tttgaagact cgaaaatttc tacgcatctc acaaaagaaa tgtatgaaaa
    17401 ccgaggaata atcatcaaga tgaaagaagg attagcaaga ttgatttcgc cgctccttta
    17461 aaacacaaaa aggacatgag aaccatcaac agatggagat ctcgtgtcct tttctaacgg
    17521 ggccatttct gagagtacgt cagaaatgga gaaagcgtct cgcatgagcg aaattaactt
    17581 tggggtatct cttgtttttt ggggaaagca agaaataagc taatatatgc tattaaaaga
    17641 gacacatttc aaagccagta tagcatagaa acttttaatg agatagtttt ttttacaatt
    17701 atgaggattt attttgttat ttaatgattt tgattcgttt tcatcaagta atgaaaatga
    17761 taaaataaac atactttagg attaaaggag atttctaatg aaaaaactta aattattctt
    17821 accaataata ggattagcta ttcttttatc aggctgttca aagtggattg atcgaggaga
    17881 atccattacg gctgttggct cttctgcttt acagccttta gtagaaacag ttgctgaaac
    17941 ttaccagaca gaaaatcctg gaaaattcat caatgtacaa gggggcggaa gcggaactgg
    18001 tttgtctcaa gtacagtcag gagcagtcga catcggaaac tctgatttgt ttgcggaaga
    18061 aaaatcgggg atcgatgcct cagcattagt cgatcatcga gtagcagttg ttgggatcac
    18121 tcctattgtg aacaaggata ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact
    18181 atttacaggc gagatcaaaa actggagtga agtcggtgga aaagatcagc cagtagttat
    18241 attgaaccgt gcttcaggaa gcggaacccg tagcacgttt gaaaaatggg tattggacgg
    18301 agaaacagct gttcaggcac aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc
    18361 cgacacaccc ggtgcaatca gctatgttgc tttttcttat gtttccgatg atgtagatac
    18421 gttgaatgtt aacggtgtag aaccgacaga tgaaaatgtg acaacgaaca aatggacgat
    18481 ttgggcatat gagcatatgt acacaagaga tcatccaaaa aaactgacaa aagaattttt
    18541 agactatatc ttatctgatg aagtacaaga taacatcgtt ggagaactag gttatatccc
    18601 tgtttctcat atgaaagtcg aacgtgattg gcaaggaaat atcgttaaag agtaatcttt
    18661 acacaaaatt tacaaactta gctaaacttt tacatgttat taaaaccaaa ttgatgttca
    18721 atttgtacac tgagtgagta tgttaaacct gattaagggg gagtcatctg tggaagatgt
    18781 gaaaaaagta ttattaacaa aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat
    18841 cagtttttta tgtatcgcgc tgatcgtgtt ggttgtatta tctatttttt attttgtagc
    18901 tagtaaagga ttggccacct tttttactga taagatcaat gtatttgatt ttctgtttgg
    18961 tacagattgg aatccaagcc aaatcggagc aaatgggaaa ccaatggtag gggcattgcc
    19021 tatgatcaca ggttcgttta ttgtgacctt tttatctgct attgttgcca caccatttgc
    19081 cattggtgca gcagtattca tgacggagat ctctcctaaa aacggaacga agatcttaca
    19141 gccggtcatc gagctgcttg taggtattcc ttctgtcgtt tatggtttta tcggtttatc
    19201 agttatcgtg ccttttgtca gaagtatttt tggcgggaca gggtttggta tcttagctgg
    19261 tacatttgtg ttgttcgtta tgatcttgcc gactgtcaca acgatgactg ttgatgcatt
    19321 aaaggcagta ccacggcact atcgggaagc ttcattagcg cttggtgcca caagatggca
    19381 aacaatctac aaagtcgtgc tacgagcagc cgtgcccggg attttaacag cagtcgtttt
    19441 tggtatggct cgtgcgtttg gtgaagcctt agctatccaa atggtcatcg gtaatgcagc
    19501 attgatgcca acaggactta ctactcctgc ttctacattg acgtccatct taacaatggg
    19561 aatcggaaat acaatcatgg gaacagttga aaataatgtg ttatggtcat tagcgctgat
    19621 cttgctattg atgtcattat tctttaatat cgtgatccgt atgattggga agaaaggaga
    19681 gctgaaataa tgaacgcaaa acgctcagat aaaattgcta caggtgtttt atatgtagtt
    19741 tcggggatca tcgtattgat tttagcagct ttactacttt atatcttagt tcggggaata
    19801 ccgcacattt catggtcctt cttgaccgaa ccttccaaag cctaccaaga aggcggtgga
    19861 atcgggatcc agctatttaa ctcgatttat ttgcttttga tcacgatgat catcagtttt
    19921 cctatttctt taggtgcggg tatctatctt tcagaatatg ctggaaaaaa ctggctgaca
    19981 gatgtgattc gcacgtcgat tgaaatcttg agctcattgc cttctgtcgt tgtcggttta
    20041 ttcggtttct tggttttcgt tattcaattc cagtatggtt tctcgattat ttctggtgca
    20101 ttggctttga ctgtattcaa cttgcctctt ttgactagaa cagttgagga gtctttacaa
    20161 gcggtccatt acacacaacg agaagcagga ttggctttag gtttgtcacg ctgggaaaca
    20221 gtgataaaag tggtggtttc agaagcgaca ccagggattt tgactggtgt gatcctaagt
    20281 tctggaagaa tttttggtga agcagctgca ttgatctata cggctggaca aagtgcacct
    20341 gcgttagatt ttacgaactg gaatccatta agtgtttcaa gcccgatcag cattttccgt
    20401 caagctgaaa cattagctgt ccatatttgg aagatcaata cagaaggcac gatgcctgat
    20461 ggtgtttctg tttcggctgg cgcttctgcc gtgttgatca ttgctgtttt actgttcaac
    20521 ttcggcgctc gtgcgattgg caaacgatta taccgaaaaa tgacttcagc ctaaggagaa
    20581 gaagcaaaat gaaaaaatat aactggaatg aaacaaatgt catcaccatg ccaaaagaga
    20641 aagaagtagc actatatacg gatgatctac atgtctggta tggcgacaat gaagcaatca
    20701 aaggagtgga tctggaattt gaaaaaaata agatcactgc actgattggt ccttccggct
    20761 gcggaaaatc tacgtatctt cgttcgttga atcgaatgaa tgatgggatc gctaatacga
    20821 aaatcactgg gaaaatcatg tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt
    20881 atgagatgcg caaacggatt ggcatggttt ttcaacgtcc taatccgttc agcaagtcga
    20941 tttatgacaa tattacattt gctttgaaac agcacggcga aaaaaacaaa aagaaattag
    21001 atgaaatcgt tgaaacaagc ttgaaacaag ctgccttgtg ggaccaagta aaagacagct
    21061 tggacaaaag tgccttggct ttatctggcg ggcaacagca acgtttatgt attgctcgtg
    21121 cgatcgcgat gaaacctgat atcttgctgc tagacgagcc ggcaagtgct ttggacccta
    21181 tttcgacagg aacggttgaa gaaacacttg taaacttgaa agaagactat acgatcatca
    21241 tcgtgacgca taacatgcaa caagcggctc gtatcagcga ctatactgcc tttttctatc
    21301 taggaaaagt aatggaatat gacaaaacta acaagatatt taccagacca aaaattcagg
    21361 caaccgaaga ttatgtgtcc ggtcattttg gttaagacgg aggcctcaac atgacaaaag
    21421 aaattatttc atctaaagat cttcatctgt attatggaaa gaaagaagca ctaaaaggga
    21481 tcgatctaag tttcaatcaa ggggaaatca ctgcgatgat cggtccttct ggttgcggga
    21541 aatccactta tctgcgttct ttgaaccgga tgaatgacct gattccgaat gtaacgatca
    21601 ccgggagtgt tttatataaa gacaaagaca tctatggtcc aaaaacagat gttgttgaac
    21661 taagaaaaga aatcggcatg gtattccagc agccaaaccc atttcctttt tctatctatg
    21721 aaaatgtcat ctatggattg aaattaaaag gggaaaaaaa taaaaaagtc cttgatcaag
    21781 tagttgaaga aagcctgaag gcagcgtctg tttgggatga tgtgaaagac aaattgcata
    21841 aaagtgcttt atctttatcc ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg
    21901 cagttaatcc ggaaatcatc ttattggatg aaccaacaag tgcgcttgat cctgtttcta
    21961 ctgggaaaat cgaaagtatg ctgttagaat taagagagcg gtatacgatg atcatcgtga
    22021 cccataatat gtctcaagct tcacgtattt cagataagac ggcattcttt ttagatggtc
    22081 acttgatcga atacaatgat acgaagaaga tattcatgaa tccagaaaaa caagagacag
    22141 aagattatat ttcaggtcga tttggataag gaggaaacgg aatgctacgt acacaatttg
    22201 aagaagaatt gctgaatctc cataatcagt tctacgaaat ggggatgatg gtgagcagcg
    22261 ccgtgcacaa gtcagttcgt gcatacatca aacacgataa aatcttagcc caagaagtta
    22321 tcgaaaacga tataaatatc aataacatgg aaacacgctt ggagaagaaa agttttgaaa
    22381 tgatcgcatt gcaacagcca gttacaactg atttgcgtat gatcattacg gtcatgaaag
    22441 ctagctcgga tttagaacgt atggccgatc atgcggtgtc tgttgctaaa tccacgattc
    22501 gcgtaaaagg gcagacgaga ataccggaaa tcgaaaaaga gatctccgat atgtccgatt
    22561 atgtgaaaaa aatggtggac aacgtcctag ttgcttatgt caaaacagac gaaaaagacg
    22621 cacgtacgat cgccaatatg gatcaaagag tcaatgacta tttcaatcga atctataatg
    22681 caacaatcaa aaatatgcag gaaaatcctg agacagtcat cagtggaaca gactacttga
    22741 atgttgccag ttatctagaa cggatcggag actatgtgac aaacatctgt gaatggatcg
    22801 tctacttggc tacagggaaa atcactgagt taaatacgaa ccacaatgag attttctaaa
    22861 ctaaagcggt gattctgccg tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa
    22921 aaagctgttc tgcatcaagt aataagaaca gtcaaacaaa aatagctcct catatttttc
    22981 atttggctgg acttattacc gcagatgcaa gcttttgaac actgtttatt cgggaaaggg
    23041 tttgtgacaa aatatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca
    23101 aaaaaaaatt agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca
    23161 gcatcaagct gctttatatg aagtacctgt catcgagatc gaaaaagtac gaataaaaga
    23221 ggatcatcaa ttgattacag gaagactgat gaacacgaat caaaaaggag aatcgattta
    23281 tttatcggtt ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat
    23341 gtatctagtc caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg
    23401 gcttttcttc ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacatgg
    23461 tctatttgct ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat
    23521 ctttactaaa atagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg
    23581 gatgattatt agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc
    23641 agctgcagtt acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatctttg
    23701 gaaagaccaa ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt
    23761 ttttctttct agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt
    23821 cattgcgaca ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg
    23881 gctttcggga aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt
    23941 cgtctatatc agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt
    24001 caaagaaacg atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt
    24061 aggaatagta ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa
    24121 acgatgaatg tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc
    24181 ggtacgcaag gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcttg
    24241 ttgttattcg gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta
    24301 ttgattgctg tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct
    24361 tttgtgagta tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt
    24421 ttagcaatcc aaggttttac aacgatagaa ctagaagaat tatctggctt agataagact
    24481 ttagcgattg attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct
    24541 gttttagatg cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg
    24601 caattatctt tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg
    24661 tcaacagtaa tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg
    24721 tttattgatt tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa
    24781 tatacgatgg ctattcttac cacattgtcc gcactacttg ttcttccttt aacctgcgtg
    24841 actacagcat atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa
    24901 cttaagtcct atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg
    24961 caaacaattt tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta
    25021 gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact
    25081 gaaaaagacg aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa
    25141 aaagatcagg caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag
    25201 ccagttgatc caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat
    25261 gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc
    25321 gctgacaata aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa
    25381 gaagaattag gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa
    25441 atcaaagagt tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca
    25501 gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag
    25561 tttgaattgc cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa
    25621 aaaatgtcag aagctggatc acagttagga aaattcttga agaaaacatt ccaaactgtt
    25681 tcagaaacag tgaatgacaa catggaatgg aaagatgtca gcttgcgtgt tcctgggatt
    25741 gcgacaacga aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat
    25801 atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt
    25861 gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa
    25921 cggagccaga ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg
    25981 cgtgcagacc ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt
    26041 ctaaatggca acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg
    26101 aatggtaaca tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac
    26161 ggcaatatcg atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt
    26221 acagtgacat ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta
    26281 cgtcttacga ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat
    26341 gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga
    26401 agtatcaaca gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac
    26461 caaatgttgc aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg
    26521 acaacaggaa gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt
    26581 cagccttgag caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg
    26641 ccttattgcc gataggctat ctcttgaaca ccgtttacca agagattatg aacttgccaa
    26701 ttatcgccga gtcacaagga ataatttcaa aaaaagctcg tcacgtttta tgaatatggt
    26761 aatttgtgac cgagaatttt gcaaaaagac accgattaag aagaaggttg tcacaacgat
    26821 tttcttcatt taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat
    26881 ccaataaaaa tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg
    26941 atccgactgt tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa
    27001 tcattttata cattttatta gctttgatta tcccatctgc gcgttctgca cgaacaaact
    27061 atggacataa caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa
    27121 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
    27181 gtactttcaa cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc
    27241 tgcgatggtc catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt
    27301 gttgaatatg tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac
    27361 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt
    27421 gggagcagaa cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat
    27481 gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc
    27541 gaactatcgg cgcagttttt ttaacagtaa agaaagtata aaaagttaag ggctaaaaag
    27601 gctgcactac cagtctttta gcccttttca ttttgttttt gaacaagtaa aatattacta
    27661 tgaaagcgaa tattctcgaa gcaatctttt ttgacgaaca ccataattgg gataactttg
    27721 ttgagaaata cgggcgaaag attcggctag tcgtcaaacg ggaagtaaac aagtttagat
    27781 attgtggaga tattcaaaaa ggataccgac tgtttgtctg cgagggatgt catcaaacaa
    27841 agtttgttcc cttgaaatgt cgtggaaagt tctgtccaac gtgtgcaaca ggtgaaagcc
    27901 aaaggtggtc agcggttgtg gcgaatgata tgtttaaagt gacacatcgg cacgtaatct
    27961 ttacgatcga tgaggggtta cgggaaatct ttatgatgga aaactatcga gattccttac
    28021 tgaaaggact aatggatgaa gccgcaaaaa ttattcttgc ctattttgag aaaagaaagg
    28081 tgaaggcagg tatagtagct acaatccaca cctttggatc aaaactagaa tttaatccgc
    28141 atgttcatat ggttgttact atgggtggct taacaagaga tggaagttgg gaagaatacg
    28201 attatttacc tttttctatg ttaagaaaat actggaaaaa tgctgtattg aaactaatta
    28261 gacgaacgtt aggtgagtgg gacaagcaaa gagttcaatc aagattacaa gctgcgtaca
    28321 agaacaatgg agaagggttt tacgtaaatg ctcctaaaag gagcaggaca aatctaaagt
    28381 cactactcca atacatcagt cgttatatga agcgtggccc gatcgcatta agccgaatct
    28441 taatgtatga tggcgacact gtcatgttca actacatgga caaacgaacg aatactaaag
    28501 agacgatgac catgagtata gacgaattca tagctgcact gattcgccat attcaaaata
    28561 agaatttcaa aacgattcgt cgctatggaa tctatagtcg tagaatcaaa acattaatga
    28621 aaaaggtcat gaaggagtat cagaaacgag taaaaaagct gtttatcaat ctgaatgagg
    28681 ccttaagacc aaaatcctgg gaagaaagaa taaccgaaga atttggtgaa aatcccttag
    28741 aatgttcttg ttgcggtgag tactatgaat tcgttgggat gagtgtgcgc aaaaatggac
    28801 ggttagttat taaatatgcg aaagataacc atgcaaagcg gtttatgcga gaggagaatc
    28861 gaaaaattga ccaaaaagaa ttcaggcaac agtatgaaga agaaaaagaa aaaactttta
    28921 aagcaattcg ctgggattgg gaacgccaac gtgaaattta tatgtctaag ttgtggtgaa
    28981 aaagaatcaa tcccaatgga tgtagtaaaa tttttagatg atgcagatat tgcaaatgat
    29041 ccgaacaatc ctccccaatt tacctgtgaa aaatgtggga aggagatgta tccagaatat
    29101 tatagaaacg cccttggaat agagtttaag atcagtgatg ttcaataaga aggttaatca
    29161 tacaaaaagt ccaccggaga attcattctc cggtggtttt cttatttaga atcaggaatg
    29221 cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta gtacttttgg
    29281 ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca tgaatgtcag
    29341 aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg acaggcgacg
    29401 agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga ctagagttga
    29461 ctggttattt taattattat tcccataatc gtttacagct atttggcagt aaagaaatca
    29521 gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga ttatgtagtg
    29581 aagatatgcc cgcatttatt atttcaagag gattaccagc tccagaagaa atgatccagg
    29641 cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct cgcttattag
    29701 gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc cacggtgttt
    29761 tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata ggaaaaagtg
    29821 aaacagcgtt ggagttgatc aaacgcggtc atcgattgat cgcagacgat cgagtagatg
    29881 tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg gaacatctga
    29941 ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg agtgcggtac
    30001 gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa gataagaaat
    30061 acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtaggc attccacaaa
    30121 taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta gccgcgatga
    30181 atttccgtgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa agattaagtc
    30241 gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt tagcacaaat
    30301 caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat tgatcatcgt
    30361 atcaggtatc attatcgcca tgtggcttag cagcagagag gctgttcggg taggattaaa
    30421 agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta ttggcgctcg
    30481 cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc aaatattcaa
    30541 tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga tcacgctgta
    30601 tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg ctgcaccaag
    30661 tgtattatta gcacaagcaa tcggtcgttg gggaaatttc atgaaccacg aagcatatgg
    30721 accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca tcattgataa
    30781 tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac tttggagttt
    30841 gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga aaaaaggtga
    30901 agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg aaggattacg
    30961 tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat ctgctgtgtt
    31021 atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt taccggatta
    31081 tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt aaaaatgaaa
    31141 aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca ggctcttggg
    31201 gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt tggggacatc
    31261 gtgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat ttaccagatc
    31321 attgtctacc cgattccatc caagcattca ccaatatgga agatgcagta aaagacgcag
    31381 atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa gaactagtgc
    31441 ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa caggggacac
    31501 ataaaaggat ctctgatatc ttgttagagg aaatcccagt agaaaaaaga aaagctgttg
    31561 ttgttctttc tggaccaagc catgcagaag aagtagcgaa acatgacatc actacgatca
    31621 cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc atgaataatt
    31681 attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca gctttgaaaa
    31741 atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat aatgcaaagg
    31801 cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca atgggtgcca
    31861 acccgctgac ctttatcgga ttaagcggag taggcgatct agtcgttact tgcacaagtg
    31921 ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc ctagaagagg
    31981 tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tacccaagca gcgatggaac
    32041 tagcccaaat aatggaaatc gaaatgccca tcactcaaac aatctatgaa gtgctgtatg
    32101 aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatggg aaaaaagaaa
    32161 atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtca aaaaagcagt
    32221 tattccagca gcaggattag gaacaagatt tttaccagca acaaaagcta tggcaaagga
    32281 aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag cgttgaaatc
    32341 aggtatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg aagatcactt
    32401 tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg aattattgaa
    32461 attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc atccaaaggg
    32521 tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccat ttgtcgttat
    32581 gctaggcgat gatataatgg aagacgaagt accattgaca cagcaattaa tcgatgatta
    32641 cgaacgtact catgcttcta cgatcgccgt tatgcaagtt cctcatgatg aaacttcaaa
    32701 atacggaatt atcaatccag gagaagtatt ggaagatggg ttatacaatg tcaaaaactt
    32761 tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg gacgttactt
    32821 attgactcca gaaatttttg atgtattgtc cgagcaacaa ccaggagccg gaaatgaaat
    32881 ccaactaact gacgcaatcg atacattgaa taaaacacaa cgcgtatttg cccgtaaatt
    32941 cacaggtaaa cgttacgatg tcggtgacaa atttggtttt atgaaaacaa gtattgaata
    33001 tggtctaaca catccagaag taggtgcacc tttacgtgag tacatcctgt ctttaggtaa
    33061 aacattagaa gcagaaggaa gcaagatcga tcataatcca aaaggaataa cagaaatcaa
    33121 agaagaaaag aaaaaataac ccttttttct aacgagcttc agctgctaat tgaataaaaa
    33181 gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct tatagaaaag
    33241 agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggattg atcgcagcaa
    33301 tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt gttgcaagtt tctaaaactt
    33361 tagaaaaagt agatcagact gtagcagaag caaatacgac gatcgaaatc gtcacaaaag
    33421 acgtagatct tttgtctaga caagtagaag gcttactggt caaaagtaat gaactgctct
    33481 tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt gctgatttaa
    33541 gtgaaagtgt atcagaactt aatcattcta gtaaaaacat cgcgacgaaa gtcggcggaa
    33601 tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt cggtgggaca gcgatgaaat
    33661 tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataaaaatgg ctaaaaaagg
    33721 tggattttta ttaggtgcag tagtcggagg cacagccgca gcaatcgccg cgttattgtt
    33781 agcaccaaaa tctggtaaag aacttcggga tgaattagct gcccaagcgg atgatttgat
    33841 ggatatggct tcagattaca cagatatggc gaaagaaaaa tcgactgagt taacaggtat
    33901 tgcaaaagaa aaagcgcaag atctttctca acaagcaggt gatctagcag gtagtgtcaa
    33961 acaaaaagta agtgaatcag cagatgaagc aggaagtgaa tctgacgata tcttgttaaa
    34021 tcttaaagaa caatcagctg acttatctaa tcgtttcaaa aaaacggcag aagatatgaa
    34081 aaaacctgca gatgaattag gaagtattgc taaagatact tcagaagata tcatgattga
    34141 tgtaaaagac gctgctggta aagcaggaga tactgtcaat gaaggaatgt cagaagcaaa
    34201 accagttgca gatgactcaa aagaaatgat gaaaatggca aaagaagatg cgaaagaagc
    34261 taaaaaagaa ataaaagata caatgacaga ttcatctaca aatcactaaa atcacaaatt
    34321 tatagaataa aaaggtggga gcggactagc tctcaccttt tttactctta tttttctaaa
    34381 acaagta
//
LOCUS       DACDRK010000021        34173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDRK010000021 DACDRK010000000
VERSION     DACDRK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.021"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            326..2491
                     /gene="acm"
                     /locus_tag="IWJ77_001141"
     CDS             326..2491
                     /gene="acm"
                     /locus_tag="IWJ77_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9452774.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2763..3248)
                     /locus_tag="IWJ77_001142"
     CDS             complement(2763..3248)
                     /locus_tag="IWJ77_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9452775.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3728..4369
                     /locus_tag="IWJ77_001143"
     CDS             3728..4369
                     /locus_tag="IWJ77_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9452776.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4388..5023
                     /locus_tag="IWJ77_001144"
     CDS             4388..5023
                     /locus_tag="IWJ77_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9452777.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5041..5856
                     /locus_tag="IWJ77_001145"
     CDS             5041..5856
                     /locus_tag="IWJ77_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9452778.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6175..7611
                     /locus_tag="IWJ77_001146"
     CDS             6175..7611
                     /locus_tag="IWJ77_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9452779.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7800..10247
                     /locus_tag="IWJ77_001147"
     CDS             7800..10247
                     /locus_tag="IWJ77_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9452780.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10477..11544
                     /gene="rlmN"
                     /locus_tag="IWJ77_001148"
     CDS             10477..11544
                     /gene="rlmN"
                     /locus_tag="IWJ77_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9452781.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11737..12840)
                     /locus_tag="IWJ77_001149"
     CDS             complement(11737..12840)
                     /locus_tag="IWJ77_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9452782.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13393..13941
                     /gene="aac(6')"
                     /locus_tag="IWJ77_001150"
     CDS             13393..13941
                     /gene="aac(6')"
                     /locus_tag="IWJ77_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9452783.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14487..16148
                     /locus_tag="IWJ77_001151"
     CDS             14487..16148
                     /locus_tag="IWJ77_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9452784.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16218..16565)
                     /locus_tag="IWJ77_001152"
     CDS             complement(16218..16565)
                     /locus_tag="IWJ77_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9452785.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16649..17590
                     /locus_tag="IWJ77_001153"
     CDS             16649..17590
                     /locus_tag="IWJ77_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452786.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17591..18634
                     /locus_tag="IWJ77_001154"
     CDS             17591..18634
                     /locus_tag="IWJ77_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452787.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18640..19710
                     /locus_tag="IWJ77_001155"
     CDS             18640..19710
                     /locus_tag="IWJ77_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452788.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19700..20653
                     /locus_tag="IWJ77_001156"
     CDS             19700..20653
                     /locus_tag="IWJ77_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9452789.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20665..21675
                     /locus_tag="IWJ77_001157"
     CDS             20665..21675
                     /locus_tag="IWJ77_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9452790.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21960..22781)
                     /locus_tag="IWJ77_001158"
     CDS             complement(21960..22781)
                     /locus_tag="IWJ77_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9452791.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22878..23156)
                     /locus_tag="IWJ77_001159"
     CDS             complement(22878..23156)
                     /locus_tag="IWJ77_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452792.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23424..25433
                     /gene="metG"
                     /locus_tag="IWJ77_001160"
     CDS             23424..25433
                     /gene="metG"
                     /locus_tag="IWJ77_001160"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9452793.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25912..26499)
                     /locus_tag="IWJ77_001161"
     CDS             complement(25912..26499)
                     /locus_tag="IWJ77_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452794.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26674..27996
                     /locus_tag="IWJ77_001162"
     CDS             26674..27996
                     /locus_tag="IWJ77_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9452795.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            28151..28927
                     /locus_tag="IWJ77_001163"
     CDS             28151..28927
                     /locus_tag="IWJ77_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9452796.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28920..29489
                     /gene="rnmV"
                     /locus_tag="IWJ77_001164"
     CDS             28920..29489
                     /gene="rnmV"
                     /locus_tag="IWJ77_001164"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9452797.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29482..30366
                     /gene="rsmA"
                     /locus_tag="IWJ77_001165"
     CDS             29482..30366
                     /gene="rsmA"
                     /locus_tag="IWJ77_001165"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9452798.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30817..31446
                     /locus_tag="IWJ77_001166"
     CDS             30817..31446
                     /locus_tag="IWJ77_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452799.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31570..32679)
                     /gene="ugpC"
                     /locus_tag="IWJ77_001167"
     CDS             complement(31570..32679)
                     /gene="ugpC"
                     /locus_tag="IWJ77_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9452800.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32888..33310
                     /gene="mgsA"
                     /locus_tag="IWJ77_001168"
     CDS             32888..33310
                     /gene="mgsA"
                     /locus_tag="IWJ77_001168"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9452801.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33633..34169
                     /locus_tag="IWJ77_001169"
     CDS             33633..34169
                     /locus_tag="IWJ77_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9452802.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT        11385 a         5915 c         7068 g         9805 t
ORIGIN      
        1 aataatgtta attaaaagtg tattttatga aactatcgtt atttattaat gttgcatttt
       61 tgtaagttaa aaattggaaa tatagattga aaaaatgaaa gcaataagta aaataaaatg
      121 aatatatgcg aaaaaataat ttacattagg aaaaaccttg taaaaacagt tttttggcat
      181 atttattttt tataaatatt tgtaaaataa ctatcaattt tttagttaaa agattggaaa
      241 tgagtggggg aggaattgaa gaaaggattt ttgagagatg atatagtagg ttttattttt
      301 agttaaaata ggggagatag aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt
      361 atttattatt actaatataa catcactgat tcctgttcat gtctatgcgg atgcaggcag
      421 agatatcagc agtaatgtca cttcgttgac cgtggaccca acgaatatta cagatggagg
      481 aaacataaaa gtaaaattct cgtttgacga aaaaaaacaa aatatccaac caggtgatta
      541 tctttggatc aattggccaa gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat
      601 cccattaatg attgaaaata aaaatgttgg aactttaacg gtcagaaaag attctgcgca
      661 ggttgtgttt aatgaaaata ttaaaaacct agactcagta gaaggttggg ggcaatttga
      721 gatacaggcc agaaacgtaa ccgataccgg tgaagaaaat acgggaccat tcaccgtgac
      781 gagcggtgat aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt cttcttctag
      841 cgttttttat tataaaacag gagacatgct acctgaagat actaagcaca ttcgatggtt
      901 tttgaatatt aacaataacg gcacttatgt tgaacaacca gttaaaatca gtgatgaaat
      961 ccaaagtgga caaagattag atccaagtac gtttgaaatt aatcaaatac acttaggtga
     1021 acagaaggtt tatagaggag aagagggaat ccaacaattt ttacaagatt tccccagcgc
     1081 tacatttaat ttttcagtaa ctgataatta cattgaaata actataccaa aaaactttgt
     1141 caatcttagg aaaataatgg tgagttataa gacgatcata gaaaacccag aacaaataaa
     1201 ttttgagaat cattcggaag cttggtttaa agaatttaat aaacctgctg ttgatggaga
     1261 aagtttcaat catacagtta aaaatatttc agcttctggt ggagtcaacg gtacagttag
     1321 aggcgaattg aagattttca aatatatcaa tgacacagag attggtatcc caaatgtgac
     1381 tttcgaatta agacgagcag atgaacagcc tattcaagga cagtctagta tcctgttgac
     1441 ttcgaatgaa cagggagaag ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa
     1501 agaaaaagaa gcacccaatt ggattgattt tgatccacta agttcaaatg aactcaaatt
     1561 ctccataaac gaaaatgata cagagggagt cagtttacct atttataaca agaaaaaggt
     1621 tacgaacatc acagcaacta aaatttggaa cggtggaacc actccaagac cgagtattta
     1681 ttttaaatta ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa
     1741 acgattagac aatggaataa cttcagtgac ttgggaagat atccaacagt atgatgatag
     1801 tggaaacgaa tatacgttta aagtgcaaga agtagatcaa aacggaaatg attatgtccc
     1861 ttcaggttat caaaaaattg aaaacgggct agtcgttaca aatgagaata aagaagtcat
     1921 ttcagtctca ggacaaaaaa catgggaaga caatgagaac caagacggca aacgaccgac
     1981 gacgatcaca gtaaatctat tagccgacgg tcaaccgatc caacacaaag aagtaagtga
     2041 aaaagatgat tggcgctatc gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat
     2101 catctatact gtaacggaag acaacgtgcc cgaatacagt acgacgattg acgggtataa
     2161 catcaaaaac agttatacac cggaaaaaac aaatatatct ataccaggta gccatataac
     2221 accgtggaaa aattttccag agaagacaga gagagagaaa attagaaatc tattctctca
     2281 aaaattgcaa atatttgctc atacaggaga atccagcgaa caaagaagca attatattag
     2341 taagagagct gtacctaaac agataacaaa taaatcaaaa agcattcttc ctaaaacaag
     2401 tagtaaaaaa tctagttttg ctgtaatcat tggtttattg atagtaacta tctgtgctgg
     2461 gatattctta caaaaatata aaaaagttta gttagaatat aatatttttt gttaaatatg
     2521 actgaaatca aaagaataaa attccgattt ataatcgaaa aaagaagcat ttttatatat
     2581 gcttcttttt tcgtagaatc aataaatatg gatttggcta actatggaag aatactcgta
     2641 ttagttttca gcgttcatac tagttgttac attgtttgat tggccaaaag aatttctgat
     2701 atattttgtg ctaaataaag aaaaagacgc ttgaattggg aaataagaaa actactctat
     2761 cattacaaat tttcataaac tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg
     2821 gaaaaatcca taatcaacgt agtggtattg atatttggat gaaagcctac ggtatcttga
     2881 cgatcaatag cagcatcgat cacgacttgg atttttttct ctttgtcgtg catcaatgct
     2941 aatggagtca ctgtacctgc tggaacgcct aggaggtcag gaagctgttc ttcagaaaca
     3001 aaagataagc gcttttcttt taattgttca gctagttgtt ttaaatcggc gtgcttttca
     3061 tcaggcaaga tgacaagata gatttgtttt ccttttcttg atttcaataa tagatttttt
     3121 acttgcggac caggtaggga aaaagggaca tttttgactg acgtaatcgc aggatgatcg
     3181 actttttgaa aagagatacc cagttttttt agtagttcat aagcttcttg ttcggttgca
     3241 cgactcatcg cgcatcaccc tccttttttt attagtgtat cataaaaaag agttgacaaa
     3301 tgaaaaataa tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga aaagaagagt
     3361 agccgttagg tgtttttaga gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga
     3421 aacacatctt ggagtcgatt ttctgaagcg tatagtagga aaatccgggg agcccgttac
     3481 agctgcggtc gttgctagat gaccagataa ggctggcttt tccgtgagga gtcggtaaaa
     3541 aaaggtggaa ccacgtgatt aacgtccttt gatcttcgga tcaaaggact ttttgttttc
     3601 atctgaacgg caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga
     3661 accacgtata atacgtcctc ttggcatttg ccaagaggat ttttttattt taaaggagga
     3721 aaagaagatg gagtatttga ttgaagttat gccgcaatta ttagtaggag cactcacaac
     3781 attgaaatta tttttattta ccttgattgg ctcattacca ttagggattg ttttagcatt
     3841 aggattgtct ttgcatttta agccgctacg ctacttgctt caaacatatg tatgggttat
     3901 gcggggaaca ccgctgttgt tgcaactgat ttttattttt tatgggttgc cgacgattgg
     3961 gatcgttttt gatcgtttcg aagcagcact cattgccttt atcttaaact atgcagctta
     4021 ttttgcagag atctttcgtg gagggataca atcgattcct caaggtcaat atgaagcagc
     4081 gcaagttttg caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac
     4141 aaagattgtt ttaccttctg tagggaatga agttatcaac ttggtcaaag attcttcctt
     4201 gatctatata ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga
     4261 cgcgacttta ctgcctttgc taggcgtggc aatcatctat cttacattga ctggtattct
     4321 aacaatcgta atgaaacaag ttgaaggaaa attaaattat taccgctaac tgataggagg
     4381 aagaaatatg attgaattga agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa
     4441 agatttgaat ctaatcattc cagatgggaa aacattagct atcgtaggtc cttccggtgg
     4501 cggtaaaacc acgcttttac gtatcttggc tggcttagaa agttctgaca gcggagaatt
     4561 ttatttagac ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac aagttgtagg
     4621 agtcgtattc caagacttcc agctatttcc acatctgaat atttttgaaa acattacctt
     4681 agcaccaaaa atggtattga aacagacaaa agaagaatac accaaaaaag cagaagaatt
     4741 agcggcttca ttagggatta gtgatttatt gaatatctat ccttatcagc tttctggcgg
     4801 acaaaaacag cgggtagcga ttgccagagc attggctatg aatccacggg tattggctta
     4861 tgatgaacca acaagtgctt tagatcctga attacgccag caagtcgagc gtttgatcct
     4921 atcattgaaa gaagatgggg taacccaaat tattgtcacc catgatttag tatttgcaga
     4981 aaatgtcggt gatcaattac taaaagtttc cccaattcat taagaaagga gaagatagag
     5041 atgaaaaaaa ccagttttct attcgctttg atcgcaggtc ttcttctttt tgcaggctgt
     5101 tcgaacaaaa aaacttcagc agataattgg gcgactatca acgaagagaa aaaaatcgtt
     5161 ataggtttag acgatacatt tgttcctatg ggatttagag ataaagacgg gaagctcaca
     5221 ggatttgata ttgatttagc caaagcagtc tttgaacaat atgggattac ggtagacttt
     5281 cagcccattg actggtcaat gaaggaattt gaactgaaca atggaacgat cgatttgata
     5341 tggaacggct attccaaaac gccggcaaga gaaaagaaag tgcaatttac aaaaccttat
     5401 atggaaaacg atcaagtgct gatcacacct aaagaatcag gaatcacaaa ctttggacaa
     5461 atgaataata agagattagg tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa
     5521 caaccagaag tattaaaaga gatagtcaaa aaccaagatg ctgttttgta tgatagtttt
     5581 aatgaagcat tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat ggataaagta
     5641 tacgcagact attatttgaa acaaagaaac gaactagaag acttcaacat aacaaaaggt
     5701 tcttatgaga gtgaggattt tgcagttggt gcacgcaagt cagatagtac attagtgaaa
     5761 aaaatcaatg aagccctaga tcaactagag aaaacaggtg aattccaaaa aatatccgat
     5821 aaatggtttg gcgaagatgt tacgccgaga aattaacaaa taggggactg tgacaaaaga
     5881 cgtgtcacag tccctttttc cgaataaaca gtgttctcct gccaactcct tgtgactcgg
     5941 agctgaatgg caggctcaca acctcttggt aaacggtgtt caaaagccag tttttcggta
     6001 ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt cttactactc
     6061 gaaactaagc gactttttca caacctcctt tacataattt tattttgttg tggaaggggt
     6121 ttcgtgttat tctaaaagag gaacgattga aggttatagg aaaggaagag atgaatggaa
     6181 cacaaagaac ttacctcatt tccaaaagat tttctgtggg gctctgcttc tgcagcgtat
     6241 caagtagaag gagcttggca agaagatggg aaaggagaat cagtctggga tcgatttgta
     6301 cggatacctg gaaagacatt taaaggaacg aacggtgatt tggcagtaga ccactatcat
     6361 cgttataaag aagatattgc tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct
     6421 gtagcttgga cacggatttt tcctaatgga cgaggagaag tcaatcaagc tgggctcgct
     6481 ttttatgaga gattgatcga cgaattgatt gaaaacgata ttgaacctat gttgacttta
     6541 tatcattggg atcttccaca agcattgcaa gatgaatata atggttggga atcacgtcag
     6601 atcattgaag atttcactca ttatgcagaa acattatttg aggcattccg cgggaaagtc
     6661 cattactgga ttagtttgaa tgaacaaaat atcttcactt cattaggtta tttgttagca
     6721 gcacatccgc caggtgtgac agacccgaaa cgaatgtatg aagttaacca tattgctaat
     6781 ttagcaaatg catctgtcat caataaattt cacgagttag aaatgcctgg aaaaatcgga
     6841 ccaagctttg cgtatacgcc aaattatcca attgacagta atcctgaaaa tgttctggct
     6901 gctgaaaatg cagaagacct gatggccaat tattggatgg atgtctatat gtggggaaaa
     6961 tatccgattg ctgccatgag gttcttagaa gaaaaaggat gggcaccaac gattgaagct
     7021 ggcgatgcag aattgctaga atctgctaaa ccagattttc ttgggatcaa ttactatcaa
     7081 acggcgacga atgcattcaa tccattaaac ggagttggtg ctggaaaaat gaatacgaca
     7141 ggaaaaaaag gttcatcaga agaaactgga gttcctggaa tgtataaaaa agttgagaat
     7201 ccatttgtcg aacgaacaaa ttgggactgg gagattgacc cagaaggatt aaggatcgga
     7261 ctgcgtcgta tcacaagccg ttaccgcatg ccggtactga ttacggaaaa tggcttagga
     7321 gagtatgaca agttgacaga agataaacaa atccatgacg actatcgaat caattactta
     7381 caaagccacg tcaaagccat caaagaagca atttcggatg gagcagaagt cctaggctat
     7441 tgcacctggt cttatacaga tttgcttagc tggctgaacg gttatcaaaa gcgatatgga
     7501 tttgtatatg ttgatcaaga cgaaacacaa gaaggatctt tggaacgata taagaaagac
     7561 agttattact ggtaccaaaa actgattgaa gaaaatggag agaaccttta gttcacatac
     7621 ttaaaacaag aaggtacggc tggatatggc cgtgcctttt cttgataaaa tcatttagaa
     7681 attaagaaaa actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa
     7741 aactttagtt cccttacttt tttttgtata atgaaacttg atgaaatgag ggataataaa
     7801 tggcacaaaa gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa
     7861 aacagcagca ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt ttatttgctg
     7921 tcttcggctt tttaaaatta ggttttttag gaatattgat tgcaaacggc tttcggatca
     7981 ttgcaggaaa tacttaccaa atcctctgtc ttttattagc cgtattagga ttttggattg
     8041 tcattaagaa tacagagttt tcaattggta aaaaccgacg ctggtttggc ggcatactct
     8101 tttatttcgg tatcttatta ttgttacatg cccatttatt tggcaaactc catactgggg
     8161 aaccaaatat catggggacg acatgggatt tgttagccag tgatataaaa caaagtcaag
     8221 tagacaataa cgttggtggt gggatgattg gagcaatcct ttatcatttt acttacttct
     8281 tgattgcaca gcctggaagt tatttagcag cagtgctatt actggcagga ggagcattcc
     8341 tgttcagtaa tctggaaggc tatcagctgt taaatggtat ccaatcaatt ggagaacgag
     8401 tacaggaatt attggaaggt gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag
     8461 agagaatgaa acagcgcgca gaagcgaaag aagcaaggcg cttagctgca aaagaagctg
     8521 cagaaaaaga agctgcagaa tatgagaaga aaaaagcggt gagccagcaa agggaaaata
     8581 ctgctgatga atggcaggaa ccagaaaata cagagccgga gcagctaagt tttgttccaa
     8641 tcgacagttt tcaagaaaat atccatcctg cgaatctaga gaaaccagta ccagatacac
     8701 caaaacagac caatacagca gaaggttttt cagatgagtt gccagaggat gacggtacct
     8761 cattagaatt tgagattgaa gcagaacagg aaaatcaaga ctatgaatta ccgacagttg
     8821 atttgcttga ttctatacca actgtggatc aaagtgatga gtataaaaaa attgaaaaaa
     8881 atatcggcgt attagaacaa actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag
     8941 ccagcttagg accatccgtc acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa
     9001 gtaaaattgt gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga
     9061 tggaagcacc gattccaggt aaatcactga ttggtattga agtaccaaat ggaaagatca
     9121 gtatggtttc ctttagagaa atcatcgaag cacaaccaaa ccacccagat aaattactag
     9181 aagtaccttt aggacgtgat gtttctggac gggtacaaac ggctgatctt tcgaagatgc
     9241 ctcacttact cgtggctggt tcgactggta gtgggaagtc agtagcaatt aatgggatta
     9301 tcacaagtat attaatgcgt gctaagcccc atgaagtaaa actgatgatg atcgatccga
     9361 aaatggttga attgaatatg tacaacggca tacctcatct gttgacgccg gtcgtgacga
     9421 accctagaaa agcagcgcaa gccttacaaa aagtagtaca agaaatggaa gaacgatatg
     9481 aaaaattcgc ggcaaccgga gtaagaaata tttctggtta caatgaattt gttcaacaga
     9541 aaaacttgga aaacggaacg aaacatccaa ccttaccatt catcgtagtg atcgtagatg
     9601 aattagctga tttgatgatg gtagccagca atgaagttga agatgcgatt attcgtttag
     9661 cgcaaatggc tcgggcagcg gggattcata tgatcctagc tactcaacgt ccaagtgtgg
     9721 atgttatcac aggaatcatc aaagcaaatg taccttccag aatggcattt gcggtatcaa
     9781 gcggtacaga ttctcggacg atcattgatt ctaatggggc agaaaagctc ttagggcgag
     9841 gagatatgct attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca
     9901 tttctgacca cgaagtagaa cgggtcgttc aatttgttac agatcagcaa gaagctcatt
     9961 atgaagagaa aatgatgccg acagatgaag ttgaaacgac aggtgctcca gaacagccac
    10021 aagatgagct gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt
    10081 cccttctcca aagaagattt cggatcggtt acaaccgagc agcacgttta gtagatgaac
    10141 tggaagctca tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg
    10201 agcaggaaga aacatcagaa acgttgccgg acgataatga catgtaaaac ttgtgtgatg
    10261 aagggttgaa aaacgaataa ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa
    10321 tggttttttt ccatcttttc taatacggta tgatgtcttg tctttttagt ttttgtgaga
    10381 ttaaagttct attcgattta tttgtgaagt attgcattaa ggaaatgaat catgtatagt
    10441 atgtagcgaa actttgagaa aaagagggaa aatactttgg aaaaacagtc catttatggc
    10501 ttgacgaatg aagaattaat caattggttt atagaaaacg gcgagaaaaa attccgagct
    10561 gcccaagttt gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat
    10621 ctatcgaaac aattgatcga aaaattatcg gagcatttta tcatcaatcc tctaaaacag
    10681 atggtcgtcc aagaagcttc ggatggtaca gtaaaatatt tgtttgaact ccctgataaa
    10741 aacatgattg agactgtact gatgagacaa gaatatggct tgtcagtttg tgtgacgaca
    10801 caagtaggat gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt
    10861 gatctgactg ctggagaaat tgttgcacag atcatgatgg tccaacatta ttttgatgag
    10921 cgcaatcttg gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga gccatttgat
    10981 aattacgaca atgtaatgga ctttttgcat atcatcaatg atgccaaagg attggcaatt
    11041 ggtgctcggc acattacagt ttccactagt ggattagcac acaaaatcaa ggaattcgct
    11101 aataacggct tgcaagtcaa tctggctatt tccttgcacg cgccgaataa tgaggtgcga
    11161 acatcgatca tgcggatcaa ccgaagtttc ccaattgaga aattgatgga agctgtggat
    11221 gagtatttag agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta
    11281 aatgatagac cagagcatgc gcaacaatta gcagatctat taaaagataa gaaaaaatta
    11341 gcatatgtca acttgattcc atataatcca gtcagtgaac atgatcaata ttctcgcagt
    11401 ccaaaagctg atgtattgag attttatgat gtattgaaga aaaacggcgt aaactgtgtg
    11461 atacgtaaag aacatggtac agatattgat gcagcttgcg gacaattgcg aagcaagcaa
    11521 atgaaaaaaa cagttgccaa ataatcgtat cctactcgtt ttttcaatta ccataaaagg
    11581 agcagttttc ttaattaagg aactgttcaa cttaaataag agaaaagatt ctatagcgaa
    11641 agctataaag ctttttttga caagaaaaac cctcatcaag caaagaaatg tcctcaattt
    11701 gatgacagcc tgcttgatgg gggttttagc aattatttaa ctgattttct tatatccttg
    11761 ctggctttct tgtgtagcat gataagtttc tacgtcaaga atggcctgtt taaatttttc
    11821 tcgggtgatt tcataaggca catgtttaat atcatttgtt gccatggctt tatctaagat
    11881 tgcttctaaa tcggtcgtga cattgagatt catgtcggtt aacgatactg gaagttggtt
    11941 ctttttcata aacacataga ttttatcaaa caaatcgaac tgctgatcca tcatcaataa
    12001 ggtcagtaca ccataactga cagatgctcc gtgaaggtat ccctccattt tttctagaac
    12061 agtcgtaccg taatagaatg agtgtgccat agcagaatta tagttattgt taactaaaac
    12121 agaaaccaat ccggtagtaa caatgatatc cgaaacgact tgttcaattg catatgaggg
    12181 acgatcgtcg gcactatctt tcaatgcttg cgtaccataa gtcaagagac gatcaatgca
    12241 catttttccg atttggattc ctaatctatc cgtgtagcct agatcatcgc cacgagcaga
    12301 aaaagtagat tcgacttctt tggataaagc atcgccaata cctgcccata agtatttatc
    12361 aggtgcttcg gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa
    12421 gaagagtccg tccattacgc cttcttcgtt atagatgaca cataccgcag tgacgggcga
    12481 acagttcgaa ccaatagtgg gaaaggtgaa gactggttta gctagttttt caccaatgac
    12541 ttttacagta tcgcaagcac ggccaccacc gactgcaaac aggacgtctg ctttttggac
    12601 actttcatgc tggatcaact gttcaatatt tccataagtt gaattacccc cgtaccagag
    12661 actggcgaca acctctattt cctgtgtcaa agctactttt attttgtttt ctgcctttga
    12721 aagtgcagtc ttccctccaa tcagcactac ttttgtccca tattctttga cgattccatt
    12781 tgctttttca tacgcatctg ttccaatggt ataatttggt aagaaaacac tttgactcat
    12841 attcatttcc ttctttcttc gttgaaacgg gttaaaattt taaagagtga tcgatctgac
    12901 tgcggctgta gatggagtag aggtgaacct aagacaggat aagtgatgaa taaggtggca
    12961 tgaacacctt gaaatcatca ataagtatcc taaaaacgaa caagtacaaa aacattgaaa
    13021 acgacaataa tgaatttttg ttcctcttag tatttctacc gattcccttt gcttttttgt
    13081 aattatctaa aaattctaaa aattaagatt attatatgaa aacaggtttt atttgtcaat
    13141 tcataattgg ttttttttaa agcaaaagat aatctatatc atgatgaaca aacagagttt
    13201 tttcctccaa ttgatggctt tcctgatctt tcctattttg aataaagtgt ttctgtataa
    13261 tttttttatt ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa
    13321 agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa
    13381 gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag
    13441 ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa
    13501 gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta
    13561 gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta
    13621 gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa
    13681 aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat
    13741 ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc
    13801 cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt
    13861 gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt
    13921 cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa
    13981 taagaaggaa tttacgaaaa ttacttcttg aatttttgtg aaattccaac ttatttccga
    14041 agaagctact tttggaacac cgtgtaccag aggttgtgaa aaagtcgctt agtttcgagt
    14101 agtaagaaaa attttgtgaa aatggctttc tacatttttg cataaatttg gcttaatacc
    14161 gagaaactgg cttttgaaca ccgtttattc agataaggga ctgtgacaag acttttgtca
    14221 cagtttcttt ttgtttattc ttttttatct ttgtatggca ataatagaca atataacgaa
    14281 aattatttaa tggcttttgg aagaccatgg aaaaccttgt acaaagctat tttttctgaa
    14341 aaattccttg aaacctcacg aaaaaatcat tattttatga aaaagacaaa aaaaagtgtt
    14401 gattttttaa taattattca ttaaactctt aaacaacttc agaaaatatt aattattctg
    14461 aaaattccaa taaaggggta cgtaaaatga aaaagaaatt tacatttggt ttggttgcgg
    14521 tatgcggcct tgtacttgct ggatgttatg gcggcagttc agccacaagc agtagttcag
    14581 caaaaagcgg ttcagcagat ggaggaggcg tatttaactt agtcgtgcct caagaaatgc
    14641 ctacagctga cttatcagtt gcaacagata caatcagttt tacagcattg aataacgttt
    14701 atgaaggaat ttaccgtctt gatgaagaca gcaaaccaca gccggcagga gctagcgagt
    14761 tggcagaagt cagtgaagac ggattaactt acaaaatcaa attacgagaa gacgcaaaat
    14821 ggtcaaatgg tgatcctgtt acagcagcgg attacgttta tggctggcaa cgaacagtag
    14881 acccagctac agcttctgaa tatgcttacc tgtatgcgcc agttgaaaat gcagaagaca
    14941 ttacagctgg aaagaaagat aaatcagaac tagggatcaa agcagttggt gactatgaat
    15001 tagaaatcaa attgacgaaa caaacaccat atttccaata tctgcttgct ttcccatcat
    15061 ttttcccaca aaaacaatcc gtagtggaag aaaatgggga tgcctatgca tctgcaagtg
    15121 ataaagctgt ttataatgga ccattcacat tagaagattt tgatggacca ggaacagata
    15181 cagaatggac ttataagaaa aatgatcatt actgggacaa agatacagta aaactttctg
    15241 aaatcaaagt cagtgtagta aaagaatctt ctactgcatt gaacttattc aaagatggcc
    15301 aagcagatga cgtgatcttg tctggtgagt tagcacagca aaacgcaaac gatccagctt
    15361 atacttctgt taaagaagcg cggacaagct atatcgaatt caatcaacgt gaaaagaatt
    15421 caccattcaa caatgtgaac ctaagaaaag ctatttctta ttctatcgat cgtgaagctt
    15481 tagtaaaaca agtactaggt gacggatcag ttgtctctac aggattgatc ccatcagata
    15541 tggtaaaaag tccaacaacg aacgaagact ttgcaaaaga agctggaaaa ttagtttctt
    15601 atgacaaaga taaagcaaaa gaatattggg aaaaagcgaa aaaagaattg ggcgttgatt
    15661 ctttagaatt tgatttgatg gcttctgatg atgattcatc gaaaaaagta atcgaatatg
    15721 ttcaaaactc aatccaagaa aacttggatg gcgtaacagt caaaccaaca cctgttccat
    15781 tctctgtacg tcttgaccgc tcaacttcgg gtgattttga cgcagtacta ggcggatggg
    15841 ctgctgacta tgcagaccca agcagcttta ctgatttgtt tgtcacaggt aattcataca
    15901 accgtggtcg ctggagcaat gcagaatatg acgaagcagt taaagcagct tcatcaacag
    15961 acgctggcga tgaacaagcc cgttgggatg atttccaaaa agcaatcaag atcatctctg
    16021 atgatatggg tgtcgttccg gtgtaccaaa aagcagaagg tcacctagtg aatgaaaaaa
    16081 tcaaaggtat cgttcaccat gcggcaggcg cttcatggga ttacaaatgg acatacgttg
    16141 aagaataatc gttaataccg tgagcatgag aaaaaacgag gctgcggcaa atgtcgcaac
    16201 ctcgttctca tattcactta tcctaacaaa gaaaaaagca tgaaaatagc agaaagagca
    16261 attagtattc cggcaatgat cagccaaaag ctgtacatac cgtagctggc agacgagagc
    16321 gaggaaaatt ctggtttttt ctttcggttc cttgttcgtg caagatggac cgatcgctga
    16381 aaatgatcga agaaacccga agaaaagacc cataaaaaac cgccgataat cagaaacggc
    16441 aatgaccata agaatagatc attagataat tgaatgagtg taaaggaatg gttgatgaga
    16501 ttttttatta aaataaccag aatcagtaga aaagctactg cgtaggggat agattttttt
    16561 ttcatttgat tgctccttta tttttggttc attatctaat aatgtagcga aacatctgtc
    16621 agaagtcaaa agctaggggg aaacattagt gaatagttat atcaaatatg tattaaaacg
    16681 tgtctttttc atgatcatca cgctttggtt aattgcaaca atcacctttt tcttgatgca
    16741 attgctgccg ggcacgccat atacgaatca ggaacgtttg agtcctgaaa caatcgagat
    16801 gctaaataaa caagtcggtt tggataaacc agttatcgta caatatggga tttatctttc
    16861 taatttgctt caaggagatt ttggaatttc cttccaattt aaaaaccagc cagtcgcaca
    16921 tttgttagct ggaagaatcg gaccatcctt gcagttaggg ttacaggcaa ttatctttgg
    16981 aacagtccta gggacgattt taggaacgat ctctgccatg aagcaaaata catgggcaga
    17041 tacatcttct acactcgtag cgatattagg tcgttccatt ccaaactttg tatttgctgt
    17101 tttattacaa tatatctttg caatcaaatt acaagttttg ccaatcgcaa aatgggatgg
    17161 ctttgtgtac acaatcttac caacaattgc gttggccatg tcgccgcttg ctgactctgc
    17221 tcggtttatc cgtacggaaa tggtggaagt actgcatagt gattatgtgg aattagcaaa
    17281 agcaaaagga ttgagccgtt gggaaatcgc ctttaaacat ggtcttcgaa atagtttgat
    17341 tccattgatg actttattag gaccgttagc tgtagcattg atgaccggat cactggtggt
    17401 agaaaatatc tttgctattc ctggtatcgg ggaacaattc gttaagtcaa tcacaacaaa
    17461 tgattatcct acgatcatgg ctgtaacgat cctttactca tttatgctga tttttgttat
    17521 tttgatcgtg gatctattgt acggtctagt tgatccaaga attcgtgttt cagaagggag
    17581 ccgaggataa atggaaatga taccaaagcg ttatgaaaca gttgcagata tccctgcgga
    17641 tgaatttctt ccattacaaa aaaataccga agaagaacgt gaacagatta ctgcaccttc
    17701 tttgaatttt atccaagatt catggcgccg tttaaagaaa aacaaagctg ccgttatttc
    17761 aatggtttta ctattggtca tcattttgat ctctgtgatc acaatctttg tttctcctca
    17821 tgatccaact gcccaaaacg tggcttatat caatttgcct cctcgtattc cgggaatcaa
    17881 tatcgatgga ttgaatggaa aagcaatggt tggtggtgaa cttgttgata aatatgctca
    17941 agcaaacgtc ccttcaaacg taaatttctt tttaggaaca gacggtttgg gacgtgatgt
    18001 attaagccgc ttatttatgg gaatgagaat ctcgttgctg atcgcattta ttgctgcatt
    18061 actagatgta acgattggtg tagcttacgg gttggtttct ggtttgttag gcggacgtgt
    18121 cgataacgtg atgcaacgtt ttttggaaat cttatcagga attccaaacc tcgttgtcat
    18181 gattttgatg ttggtcgttt ttgaaccagg aattttttca atcgtggctg ctatggccat
    18241 cacaaactgg ataccaatgg cacgtatcgt tcgtgcacaa acaatgaaat taaaagatca
    18301 agaatatgtt ttagctggat tgacattagg tgaatcaaga atgaaaattg cattcaaaca
    18361 tattttgccg aatatctcta gtgtcattat tattcaaatg atgtttagta ttccatctgc
    18421 tattttcttc gaagcctttt taagctttat cggtttaggt cttacgcctc cgtcagcttc
    18481 attaggaacg atgttgagcg aagggtacaa gacattcctg tatctgcctt atctattatg
    18541 gattccggca gtaactttat cggttatcat gattggcttt aacttacttg ctgatggatt
    18601 gcgtgatgcc tttgatccta aaatgaaaga gtgaagacga tgacgaaaat tttagaagtg
    18661 aaagacttag aaatctcttt tgatacgtat gctggaaagg tacgagcgat tcgcggtgtc
    18721 aattttcatt tgaataaagg agaaacattg gcgatcgttg gggaatctgg aagtggaaaa
    18781 tcagtgacga ccagaagcat catgcgcttg ctgtcaagca acgccaatat agatgctggt
    18841 caaatcctct ttaaaggtaa cgatatcgtt ggaaaatcag aaagagagat gcaaaaaatc
    18901 cgtggtaaag aaatagcgat gattttccaa gatccaatga cctctttaga tccgacgatg
    18961 cccattggga agcaagtcgc tgagtccctt agaaagcata ataaagtttc aaaaaaagag
    19021 ggacaaaaag ctgcattaga attgcttaaa ttagttggaa tacccgaagc tgagaaacgt
    19081 atcaacagtt atccgcatca gttctcagga ggtcagcgtc aacggatcgt gatcgcgatt
    19141 gcactggtat gttatccgga aatcctgatt gctgatgaac ctacaacagc gcttgatgtg
    19201 actattcaag cacagatttt ggagctgctg aaagaaattc agacaaaaat cgatacatca
    19261 attatcttta tcactcatga ccttggtgta gtagcgaatg tcgctgatcg tgtcgctgtc
    19321 atgtatggag gaagaatcgt tgaagttgga acatcggaag aaattttcta caacccgcaa
    19381 catccctata cttggggact gcttggttca atgccgacat tagatagtgc gaacgatcga
    19441 ttgtatgcta ttcctggttc tccaccggat ttattgaacc cgccaaaagg agacgcattc
    19501 tatccaagaa atgaattcgc gatgaaaatc gatgcagaac aagaaccacc attttttgaa
    19561 ctatcaaaaa cacacaaggc agcaacttgg ctacttgctc cgcaggcacc caaagtgaca
    19621 ccgccagaag aaatccaacg tcgctgggct atctttaaag agaaacaaaa acatacttac
    19681 ggtcaaggag gaattgctga tgccaaataa actacttgaa gttaaaggat tgaaacagta
    19741 tttcaatgta ggaaaaaaga atgaagtaca tgctgtaaat gacatcagct ttcacattta
    19801 tgaaggagaa acattcggtt tagttggaga atctggaagc ggaaaatcca ctactggccg
    19861 tacgatcatt cgcttgaatg aacctactga tggtgagata ttattcgacg gtcaagatat
    19921 aacgaagatc aaagataaaa aaggtttgac gaaattccgg catgatgttc agatgatctt
    19981 tcaagatcca tacgcttcgt tgaatcctcg tatgaaagtc agagatatca ttgccgaagg
    20041 aattgatgtt aatggactag ctaaatcatc tgaagagcgt gcaaaaaaag tcgatgatct
    20101 attgagaact gttggattga atccaagcca cggtactcgt tatcctcatg aattttcagg
    20161 tgggcaacgc cagcgtattg gtattgcacg agcattggca gtaaaaccgc gttttatcat
    20221 ttgtgatgaa ccaatttctg cactagatgt gtcgatccaa gcccaagtgg tcaatttgtt
    20281 gcaagatctc caaaaagaac atcaattgac ctatctattt attgcacatg atttgtcgat
    20341 ggttaaacat attagtgacc gaatcggtgt aatgcataac ggcttgttat tagagatggg
    20401 aacaagtgat gaaatctata atcacggtgt tcatccttat acagaaagtt tgttatctgc
    20461 tattcctcta ccagatccag accatgagag aaaacgcaga agaatcaaat atcaacctga
    20521 accagatgac ggacaagtac gtcaacttcg tgaaatcgca ccagaacatt ttgtctatgc
    20581 aactgagcag gaagtcccat attacgcaaa aaaactaaaa cgacaaaagg aatctcttct
    20641 ggttgctaac taggagggac atagatgcta aaagctttta aattccgtat gtatccgaca
    20701 gaagaacaaa aacagcaatt gattcgtact tttggatgtg ctcgatttac ctataaccat
    20761 ttgctgaaaa agcggcagaa aagttggcaa caaacaggag tagccaattt ttcgctcaca
    20821 cccgctacat taaaaaaaga atacccattc ttaaaagaag tagacagtct tgctttagca
    20881 aatgcacagc ttaatttgga tcgggctttt cgcaattatt tcaagggacg tgccagcttc
    20941 ccaaagctta aaacgaaaaa aagtatgtgg caatcttata cgaccaacaa ccaaacccgt
    21001 acgatctatc tggaaaatgg ctacttaaag ttaccaaaac aaaaagaact aatcaagata
    21061 aatcagcatc ggccagttga aggatcgatt cgctccgcta cgatttctgc tcgatataat
    21121 gaagaatttt atgtcgcact actttgtgat gtttcgccag taaaaaaaga atctttagct
    21181 aaatggatcg ggatcgccta tcatccgaag acattgatcc aaacatctcg acctctagaa
    21241 gtaactttac caaaatttca ccagacagaa gaaaagctgc aacatgcaca gcggaaattg
    21301 aatgtgaaag tccgctccgc tcaccataga aagatcaggt tagatcaggc aagcaattat
    21361 cagaaacaaa agagaaaagt aatggatctt tatctgaaac aaaagaacca gcgagaagat
    21421 tatcttgaac aattatctgg gaaattggtc aaacaatatg actacttatt tgtagaatct
    21481 tttccaaaag aagaggcaca tgctgatttt tctattcacg actggcataa attaataaca
    21541 aaattgcgtt ataagtccca atggtataat aaaaaatttc ttttgatcaa taccgacgga
    21601 gcagaagaaa gcaattccgt aagaaagagc caagtagtag aaaaaatggg cagacattcg
    21661 gttatcaagg gataaaaaat gaatgaaact gataggagcg ttagggttag ccttgttaat
    21721 aaaaagtctt aatgactttt gtttctagga atttttcttt tgaagaaaag ctcactaatt
    21781 atgacaaacc aataaaacaa tagcagaaaa agttcgtacg agctttttct gctattgtta
    21841 attttttatt cagaaagttt acgaatacat caaaattctg aaacaaaaaa cgtaactcac
    21901 aaagagttgt ttttgcgttt tttcttgaat gattaggtct gaatcttttg ctcacaacct
    21961 tattcatggt gccccggctt gagctgatag atttttgaag gacggccgat tcctacagga
    22021 cgttcaccga tctcatcgaa atattggagc atagctttct taaaattgga atgatcgatc
    22081 gttttataat cgatccctaa gaatttagca aatacttttc gagcttccgt aatcgtgaaa
    22141 tctttaccta aaacttgtag aacttgcggc tcgtgttcca ttttattgac tacacggttg
    22201 aatgctttca agatgatttg actatggtca aaagccaaag tatctttgcc agaagactct
    22261 ccagtgacta aatcaagcga gatagccact tctccgctag ataaatggat cttgttacta
    22321 tgacgttcta gtgtgaacca gcgtacttcc ttggcatcat ctccggcgat tagcggttct
    22381 tctccaataa aggctaagta gctgactgtg acgacccagc ctcgcggatc tcgattaggc
    22441 gtactgaatg tatgcagttg ctcaatattt tcttttgaaa taaccacacc agtttcttcc
    22501 tttgtttctc ttaagacgct ttctccagtt gactcatcct tttggacaaa gccaccaggc
    22561 aatgcccatg aatttcgata aggatgccct tttctttgga tcaacaaaac tttcagctga
    22621 tcttcttctt tgttatagca catcagtacg atatcgacag tcaaagaggg cttgtcatat
    22681 tctggacgtt cctgtttttg ataccagtta agaaattctt cttcacttgc tttatgttca
    22741 taatataatt tttcttcttc tttagaagca aaacggttca tctgaccacc tccattgaca
    22801 gtaaaagtac tttttacctt attgtagcag actctgaaaa aaaaccaagg atttacttga
    22861 aaaatctagg ttatcattta aatatgaaaa ataaatggaa gtatgagatt gaataaaaag
    22921 aaaagcagat aaaacggcaa tactttatac cgctcttcac gaatggcaaa gaagccacaa
    22981 ataacgatat atacgtcaat gataaaacgc aaaatccaag ctgctgtcat aaaactgttc
    23041 aaatgcccat ggaggttaaa ataaccatac atacgaatat aaaaatagct gaatattaaa
    23101 tgtgctaatg aaagaaaagc aatactaagt gtaaaaaaaa gatgtttctt ctgcatgatt
    23161 gttccagtcc cttactaaga tagatagtct ttattatacc acgtgtgagg ggagaaataa
    23221 aaagccgttt tttagtagat atgaaacaaa acaaaaaccc gcatttattc aacctattca
    23281 tccttttaaa ggatagattg aacgtagata gaagtcgttt atccagtctt tttcattttg
    23341 aaaaaaagct aacagcatgc tttttttatg gtatactagc aaggaaacga attcgcatca
    23401 tgcgataaag gaggaagagc cccgtggcag aaaaggaaac tttttatatc actacgccaa
    23461 tctattatcc aagcggcaaa cttcatatcg gcaactcgta tactacgatt gcatgtgacg
    23521 caattgctcg ttataaacgg ttgatgggct ttgatgtgtt ttatctcact ggagtggatg
    23581 aacatggtca aaaaatcgaa acaaaagcag aagaactagg tgtaaaaccg caagaatatg
    23641 tagataaaat ggcggctgat gtcaagaaat tatggaaaac gcttgatatc agttatgata
    23701 aattcattcg cacgacagat gattaccata aagcagctgt tcaaaaaata tttgatcgtt
    23761 tattagaaca gggagatatt tacttaggtg aatatgaagg ctggtactct gtttctgacg
    23821 aagaattctt tacagaaaca caacttgcag aagtgtacaa agatgacgaa ggtaacgtga
    23881 tcggtggtaa agcgccaagt ggacacgaag tagaattggt aaaagaagaa tcatacttct
    23941 tccgcatgag caaatacgca gaccgtctat tggaatacta tgaagaacat cccgaattca
    24001 tccagccaga atctcgtaaa aacgaaatga tcaacaactt tatcaaacca ggattagagg
    24061 atcttgcagt ttcacgaaca acttttactt ggggagtacc agtcaagagt gatccgaaac
    24121 atgtggtgta tgtatggatc gatgcattat ccaattatat tactgcacta ggatatggct
    24181 ccgaagatga aagtctattc gaaaaatact ggcctgcaga tgttcagatg gtcggtaaag
    24241 aaatcgttcg ctttcatacg atctattggc caatcatgct gatggccctt gatttgcctc
    24301 tacctaaaaa agtatttggg catggctggc tattgatgaa agacggaaaa atgtctaaat
    24361 caaaaggaaa tgtcgtttat cctgaaatgc ttgtggaacg ttatggttta gatgcactcc
    24421 gttattattt gcttcgtgct gttccgtttg ggtcagatgg tgtctttacg ccagaagatt
    24481 ttgtttcccg tgtcaattat gatctggcaa atgatctagg gaacttattg aatcgaacga
    24541 ttgctatgat caacaaatat tgtgatggac tcgttcctga ctatgcctca cttgtcactc
    24601 catttgatag tgaactgtct acgacggctg ctaacgttgt cggacgttac catgatgcaa
    24661 tggaaaaaat ggaattcaat acagcattgg ctgaaatctg ggtgctgatt tctcgtgcaa
    24721 acaaatacat tgatgaaaca gaaccatggg tattagcaaa agacgaagaa aagaaagctg
    24781 aattgaatag tgtcatgatc catttagctg aatctttgcg tatcgcagcg atattacttc
    24841 agccaatcat gacagaaacg ccagagaaga tcttcaatca actaggattg gactctgaaa
    24901 caatgaatct tgaaggatta cactttggcg aatttccttc aggaacaaaa gtagttgcaa
    24961 aaggtacacc aatcttccca cgtttagata tggaagaaga agttgctttt attcaagaaa
    25021 aaatgtctga aggtactcaa acaaacgaag atacagtcaa atgggatcca gaagaaacag
    25081 aacttgtttc aacgaaagaa aaacaaatca aatttgatgt atttgaaaaa gtagaactaa
    25141 aagtagcaga agtcatcaac tgtcaaaaag tagaaggggc agataagttg ctgcaattcc
    25201 gactagatgc tggagacagc caagaccgtc aaatcttgtc aggaatcgct gaattttatc
    25261 ctgatccaag cgaattgatc gggaaaaaag tcgtcattgt agcaaatctg aaaccacgaa
    25321 agatgcgtgg acaaatcagt caaggaatga tcctttctgc agaagcacca gatggctctt
    25381 tacaagtaat cgaagcacca aaatccatgc caaatggctc agaaatcgca tagaaattca
    25441 aagggggtat gacactagtt gtgtcatacc ccctttttcc gaaatttgag aagcaatttt
    25501 cgtaaattcc ttcttattac ttgaagccaa tcatataata cagaaacaga gaactatcca
    25561 aaataaatcc tagaaaaaga agtacgagct tcgctgattt ttgttaattt agaataatac
    25621 taataggaat cgtaaaaaca gaaaaaaagc ataatgataa agggtcgggc agattttaat
    25681 tgattcatct ggtactcttt tttattgtcc ttacattcat aaagtttgaa tgcaatgact
    25741 ttattacatc tatatttata tctaacagta gcgttgatgt gttaattata agttctatca
    25801 ctttatgttc aattagtttg tgctcttata tgtgtgattg aaaatataga taaaaaaaca
    25861 atcatcggct ttgacttcga tgattgtttt ctttattttg taaaaatcct atcacttgat
    25921 tttatggtaa ccttttattt ttgtcttcaa acggcttggt aagagagcag taagcagtgc
    25981 aaatgggatc gaaaagacat accagaaaat cgtacgtccg ataccataat gttgatagag
    26041 ctttgtcaac atcacgtgta acaagatcaa ccctaaaagc aaggagccag ctataaacca
    26101 atgattatcc caatgcatga cttcactaag tgccatcact ccaaaaggat agaaataaaa
    26161 atagatcccg actaaggtca tccgccagtt ctttgtaaat gttccgatat tcaataaaaa
    26221 gaaaatcgct gaaagcaaaa taccgatagg cggaaaaagc ccaaccaaaa aggaatacaa
    26281 catcccccac aagatcatca caggaccttt gatcaatgca acgattgcga tgaacaaagt
    26341 cagcaatatt agtttttgtg ataacgttgc aacagtcatt aatccaaata aaattaatgt
    26401 gaaccaatgc caatgggact gctgttgttc tttacgggaa aaatcgaccc ggttggataa
    26461 ataatctgct aatttcggat aagaacgctt aaaatccata aactgttcct ccttcgtgga
    26521 aaaggtaggt ttagtatacg aaaaataaaa aagaaataca tccgtcttaa gagggagttt
    26581 ttcactgaaa gtggagaaaa aagaatggta aattaaaata ttctcagtta tactgaaaat
    26641 tataccaaat ttcaaagaag aaagaggatg ggaatgaaag gcaaactaaa acgagaaatc
    26701 aatctgtttg gcgcattagc taccgttatg ggaaccgtga tcggtgcagg cgtcttcttc
    26761 aaaactgctg ctgttactgc tagtactcag tctacaagtt tgacattgct agcttggctt
    26821 ttgggtggtt tcttaacgat ctgtgctgga ttgacagtgg ctgaattagc tacagcaatc
    26881 ccggaaactg gtggagctgt aaaatatatt gaagctgcgt atggaaaatt gcccagcttt
    26941 ttattaggat gggcgcaaag cttgatttac tttcctgcta atattgctgc attatcgatt
    27001 atctttgcga cacaattgac gaacttatta caattatcta ccgattatct tttgttgatt
    27061 gcagtgatca ccgctgtttc tgtgacaggg ttgaatttgt tagggacaaa agtcggtgct
    27121 tctgtacaat cagtgacgtt gattgttaaa ttaataccaa ttgctgtgat tgtcatatgg
    27181 ggattattga ccccaggaca aggaactgtc cagttatttc caatcgaagc tggaaaagat
    27241 gttacttttg tcgaaggatt gagcagtgcg ttgttagcaa cgttgtttgc ttatgacggc
    27301 tggttaggtg tcggtgcaat ggcaggggaa atgaaacggc cggaaaaaga tttgcctaaa
    27361 gcaattattc ttggtttgag ttttgtaaca gtcgtttatc tattgatcaa ttttgttttt
    27421 ttgaaaactt tgccgattga tcatcttgcc ggtaatttga atgcagcttc tgaagcatca
    27481 gacgtcattt ttggaggaat cggtggaaag ctggtcacta ttgggatctt gatctcggta
    27541 tacggtgcat tgaacggtta tacactaact gggatccgcg tgccttatgc gatggcttta
    27601 gaagacgagc tacctttcag taaacagctg acaaagcttt caaagaagtt tacggttcct
    27661 tatgttcctg cggttttcca gttagcagta gcttgtatta tgatgagcct tggttctttc
    27721 gactttttaa cagacatgtt gatttttgtg atgtggctat tcagtttact gatttttatc
    27781 ggtgtcttta ttttaagaaa aaaagcgccg gaattgccac gtccatataa agtaccactt
    27841 tatccaatcg ttccgattat tgcgattctc ggtgctattt tcattttagg tatgacgatg
    27901 atgacgcaaa cgaaattagc attgatcggt ataggtgtta ccttgatagg tatcccagtc
    27961 tattatcaaa aaaagaaaaa acgctcagaa gagtaaaata tttcaacaac agacagaaaa
    28021 agtacgaact aggtcctcta gatcttcgtg ctttttctat ttcaataaaa caagttcttc
    28081 tattttgatt tttgctactt ctgcaagtag tgcttatgtg ctaaagtaag aatgaaaagg
    28141 agagataaac atgatatttg attctcatac ccatttaaat gctgaacagt tcaatgaaga
    28201 tattccagaa acgattcagc gagcacaaga aatgggggta acgaaaatgg cggtcgtggg
    28261 cttcgatacg ccaacgattg aaaagtcttt gcaattaagc catgactatc caaatatata
    28321 cagtattatc ggctggcatc cgacagaggc tggcagttat acgaaagaaa tcgaaaataa
    28381 attacaagaa caattgactt taccaaaggt tgtagcatta ggggaaattg gcttggatta
    28441 tcattggatg gaagacccga aagaagtcca agataaagta tttcgcagac agattgctat
    28501 cgcaaaagaa atgaacctac caatcagtat tcatactaga gaagcacttg aagacacgta
    28561 tcaaatccta aaagaagaag atgttcgaga tatcggtgga atcatgcata gtttcagtgg
    28621 tgattatgaa tgggcaaaac gatttcttga cttagggatg catatttcat ttagcggtgt
    28681 cgtcactttc aaaaaagcat tagacgtcca agaggctgca gcaaatgtac caatcgaacg
    28741 attacttgtg gaaacagatg ccccttatct ggcaccagtt ccttttagag gaaagcgaaa
    28801 tgaaccaggc tatactcgtt atacagtaga aaagattgct gaacttcgga atcttccgac
    28861 agaagaagtg gctgcccaaa catggacaaa tgcgcaccga ttatttggtc taagcgaaca
    28921 tggataagat gaagatccaa gagattatag tcgttgaagg aaaagacgat acaagaaggc
    28981 tgcgggaagt agttgatgca gatacgatag aaacaatagg gtctgcaatc aatgaagaga
    29041 tcctgatgca aattgaacat gcccaagaga ctcgtggagt gatcgttttt accgatcctg
    29101 atttttcagg agaaaaaata agaaaaatca ttatggaagc tgttcctcag gcaaaacatg
    29161 cttttttgcc gcgcacacaa gctaaaccaa agaaaaaggg aagccttggt gtcgagcatg
    29221 caagtgatgc tgctatttta gaagcattga aaaaggtcgt gacgccagac atttcccaaa
    29281 cggctgtttc tgaaatcact agacaagacc ttttagacta tggtttaata gctggggcac
    29341 aagcaaaaga aaaacgagaa aaactaggtg acgagttgcg catcggttac acgaacggca
    29401 aacaattgga aaaacgtttg aagatgtttc gtattacgcc gaaagaactc gcccaagcaa
    29461 tgaaaatagt ggaggaacaa catgagtgaa tatcgtgaaa ttgcgacacc ctctagaaca
    29521 aaagagattt tattgaaaca tggtttttca tttaaaaaaa gtttaggaca aaattttctg
    29581 actgaaccaa atattttaag gaaaatcgta gaaactgcgc aaatagatga acatacgaac
    29641 gtgatcgaag ttggaccagg aatcggtgca ttgacggaac aattagccaa gcatgcaaaa
    29701 caagtcgtag catttgagat cgatgatcgt ttgatccctg tattggctga tacgatgcaa
    29761 ccctatgaca atgtgactat tgtacatcaa gatattttaa agaccgattt gtcaacagct
    29821 gttagagaaa catttcatga ggagcttccg ttaaaagtcg tagcgaattt accttactat
    29881 attacgacac cgatcatgat gcattttctt gaatcagatt tggttgtgga tgaactagtc
    29941 gtcatgatgc aaaaagaagt agctgatcgt atctctgctg aaccaggaac gaaagcttat
    30001 ggttcattat cgattgctgt tcaatattat atggaagcca gtttagcatt catcgtacca
    30061 aaaacagtat ttgtccctca gccaaatgta gattcagcga ttttgaaact gacaagaaga
    30121 gatataccgg cagtcgaagt gacagacgaa aaagcatttt tccgattgac aaaagcggca
    30181 tttcagcaaa gaagaaaaac gctttggaat aacttgcagc attcgtatgg caaagatgac
    30241 cagacaaaag catggttagc aaaaagcttg gaaacagctg gaatcgatcc aaaacgtcgt
    30301 ggggagactt tgtcattaca agaattcgcc gcattaagca atgcgatgtc agaaaatatg
    30361 cagtaaaaaa agaggttgtg acagaagttg tgaaaaagct gtcctgcatc aagtaataag
    30421 aacagccaaa caaaaatagt tcctcatgtt tttcgtttgt ttgggcttgt taccgcagat
    30481 gcaagctttt gaacaccgtt taagaaagag gttgtgggcc tgccgtttaa ctctaagtca
    30541 caaggaacaa tttcaaaaaa cagcttctca tgtttttgag ttagttggtc ttattacttg
    30601 atgcaagctt ttgaccacta ttatgccgaa caaggagatg tgacacaaat tttgtcacat
    30661 ctcctatttt tgtttcttga tcaaaaaatt cttatacttg atactcacac tcattttgta
    30721 tggatttaga aaagaatatg tgtcttgaca gttattagta agtgagttat actaagtcga
    30781 catttatgtg tcaagacata ttaggagaga gaaaaaatga gaaaaaattc agttcgaaaa
    30841 ctaacgattt ctgctttatt gattgggatg ggggtcatta tcccaatggt catgcctaaa
    30901 atcatgattc caccagcttc ctttactttg gcgtctcatg tgccattatt tattgcgatg
    30961 ttttttacac caggagttgc agtggctgtt gcgctaggta cgacatttgg tttcttttta
    31021 acaacgccgg ttatcatcgc attgcgagca ttgtcacatc ttgtgtttgc gttgattggg
    31081 gcttggtact tgcaaaagca tccgagtatc gttttaaaaa atggacagtt tacttttgct
    31141 aattggcgtt tccaagggtt caattttgtg atcggtgtga ttcattcttt agcagaaatg
    31201 ctagtagtca gcatctttta ctttattggt aatatgcctg aaacatatta ttctcaagga
    31261 tatttttata cagtattcat cttgatgggg attggtgggc ttatccacag tttagttgat
    31321 tataatattg cttattttat agcaggaaca ctcagcaaat attttgatat ccccgtattt
    31381 acggcagcaa aaaaagaaaa tcggaaagag tcgattcaag ggtacgcgaa gaaaacgaca
    31441 tattagacat gatctctcaa aaaaaaaact ctcatctagt aatggataat aaatcctcta
    31501 agatgagagt tttttgtcct aaaaagagct gcaccaacat cttctggcac agctctaaca
    31561 gggggcaatt tatggaagtt tgatgacttg ttctgtttct ttatcaaaga agtggccttt
    31621 gctgatgttg aaggcaaggt cgactatttc gcctggttta tggaaatctc ttgcatctac
    31681 ttttgagatg aactcagtag aaccagtttt tgtgtaaagc atcgtttcag ctccaagtaa
    31741 ttcggaaacg acaacttctg atttcactac ggcttcaggt gaagcttcaa gggctacttg
    31801 ttcactgtgg atgtcttctg gacgaatacc gaagataact tcttttcctt cataaccttt
    31861 ttcaactaat actttgtttt ttccttcagg aatacgaagc ttaagcccat gtccatctgt
    31921 gataacacca tttttcaatg tcacattaaa gaagttcatg gcaggagatc caataaagcc
    31981 ggcaacgaac atattgacgg gtgtatcata aacttctttc ggtgtaccga tttgttggat
    32041 aaatccgtct ttcatgataa cgattcggtc agccatagtc attgcttctg tctgatcgtg
    32101 agtaacatag atggtcgttg tttcaagacg ctggtgaagt ttggcaatct cagcacgcat
    32161 ggctacacgt aatttagcat ctaaatttga taatggctca tccattaaga agacttttgc
    32221 atcccgaacg attgcccgtc ccaatgccac acgttggcgt tgtccaccag ataaagcagc
    32281 tggttttcgt ttcaagtatt ctgtcagacc taagatctct gctgcattgt ccacacgttt
    32341 tttgatgtct gctttatcat atttacgtaa tttcaagcca aaagccatat tatcaaaaac
    32401 agtcatatga ggatacaatg cgtagttttg gaaaaccatg gcaatatcac ggtctttagg
    32461 tgccacgtca ttcatgactt ttccgccgat atttagttct ccttcagaga tatcttccag
    32521 tcccgcgatc attcgcaaag tagttgactt tccacaaccg gaaggaccaa caaaaacgat
    32581 aaattcacgg tctttgattg atagattaaa atcagttaca gaataatttt ctgcattgtc
    32641 atatttttta tatacgtttt tcagagccat ttctaccata atacgttcca cactttcttt
    32701 tttagtttta ttaactattt aaagtataaa tgaaagcgtt aactaacttc aatgtcatgt
    32761 tgaacaagaa ttttttcttt tttttgtcaa tgtgctagtt ttaagcttta ataaatatcc
    32821 ataagaaata aaaactgata cactggagta aataagtatg ttaacaatat caaaaggagt
    32881 gtctcttatg aaaatcgctt tgatcgctca tgaccgaaaa aaacctatga tggtgaaatt
    32941 agcaacagcg tataaaacaa tattggaaaa acatgaactt tttgctaccg ggacgactgg
    33001 gcagaggatc acggaagcaa ccggcttgcc gatccatcgg tttaaatccg gaccgttagg
    33061 cggggatcag caaattggag ccatgatttc ggaggatcag ttagatttgg tgatttttct
    33121 tcgagacccg ctggctgcac agccccatga accagatgta acagcactta tccgtttatc
    33181 tgatgtctat gagattccgt tagctacgaa tatcggaaca gctgaggtgc tactaagagg
    33241 actggaagcc ggcttcctag attttcgaag aatcgtccat gaaatagaca agcatccact
    33301 ttcattttga tggataagcg tatggaaaaa taacgtttaa tgttcgccta tatgtagcta
    33361 taaaagctaa acaaactgat aatatgcgat ataatacgaa cgtattggat aatataaaca
    33421 ataatattag tttttcgtat ttgcagtaaa tgtgaaaata tgctatagtt tctgtgggaa
    33481 aagatcccaa aaataataca gagtagaaaa taaagcgtta agtgctaaga ggatgggatg
    33541 ttgcctcttg gacgaaggac gtaatgttcg cggttttatt ttcgcattcg ctgcatgata
    33601 cgatgtagca gctcttaaag gagatgctaa aaatgaagaa acaccggtta gacgcacgta
    33661 tgattgctat tatgggctta ttgattgcat tgatggtcac tttgtcccga ctggtagcaa
    33721 tcgagacccc atttattaaa atcagtgtca catttattcc acaagtgatc atgggcattc
    33781 tttttggtcc tttttggtca gggataggcg cagttttagc tgatttagta ggaatggccc
    33841 tcttctcgaa atctgcattc tttattggat ttacattgaa tgcatttatt gaaggagcaa
    33901 tctacggatt ctttttctat cgaaaagaga tcacttggaa aaatgccatt ctggctacgc
    33961 ttagtgtcac gctgatcatc aacttatttt tgacaccatt atggcttgcg ttgatgtacc
    34021 atgtgccatt gttcagctgg gtcgtttggg cgccccgcct gttaaaaaca gtaatatggt
    34081 taccgattca atcaattgcc atttattacg taggacgtag tattccttac aaaaaaattt
    34141 taagaagttt agcgatccac gctaaataaa aaa
//
LOCUS       DACDRK010000022        34117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDRK010000022 DACDRK010000000
VERSION     DACDRK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.022"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..480
                     /locus_tag="IWJ77_001170"
                     /pseudo
     CDS             1..480
                     /locus_tag="IWJ77_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1009..1161)
                     /locus_tag="IWJ77_001171"
                     /pseudo
     CDS             complement(1009..1161)
                     /locus_tag="IWJ77_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1429..2760)
                     /locus_tag="IWJ77_001172"
     CDS             complement(1429..2760)
                     /locus_tag="IWJ77_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9452803.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(2909..3622)
                     /locus_tag="IWJ77_001173"
     CDS             complement(2909..3622)
                     /locus_tag="IWJ77_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9452804.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            3797..5221
                     /locus_tag="IWJ77_001174"
     CDS             3797..5221
                     /locus_tag="IWJ77_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9452805.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5520..6227
                     /locus_tag="IWJ77_001175"
     CDS             5520..6227
                     /locus_tag="IWJ77_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9452806.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6214..6537
                     /locus_tag="IWJ77_001176"
     CDS             6214..6537
                     /locus_tag="IWJ77_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9452807.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6626..7663)
                     /locus_tag="IWJ77_001177"
     CDS             complement(6626..7663)
                     /locus_tag="IWJ77_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9452808.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(7984..8967)
                     /locus_tag="IWJ77_001178"
     CDS             complement(7984..8967)
                     /locus_tag="IWJ77_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9452809.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9382..10152)
                     /locus_tag="IWJ77_001179"
     CDS             complement(9382..10152)
                     /locus_tag="IWJ77_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452810.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10145..11080)
                     /locus_tag="IWJ77_001180"
     CDS             complement(10145..11080)
                     /locus_tag="IWJ77_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452811.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11396..13045)
                     /locus_tag="IWJ77_001181"
     CDS             complement(11396..13045)
                     /locus_tag="IWJ77_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9452812.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13162..15765)
                     /locus_tag="IWJ77_001182"
     CDS             complement(13162..15765)
                     /locus_tag="IWJ77_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9452813.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15777..16826)
                     /locus_tag="IWJ77_001183"
     CDS             complement(15777..16826)
                     /locus_tag="IWJ77_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9452814.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(16852..17166)
                     /locus_tag="IWJ77_001184"
     CDS             complement(16852..17166)
                     /locus_tag="IWJ77_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9452815.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17182..17628)
                     /locus_tag="IWJ77_001185"
     CDS             complement(17182..17628)
                     /locus_tag="IWJ77_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9452816.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17629..19605)
                     /locus_tag="IWJ77_001186"
     CDS             complement(17629..19605)
                     /locus_tag="IWJ77_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9452817.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(19763..20659)
                     /locus_tag="IWJ77_001187"
     CDS             complement(19763..20659)
                     /locus_tag="IWJ77_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9452818.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20652..21332)
                     /locus_tag="IWJ77_001188"
     CDS             complement(20652..21332)
                     /locus_tag="IWJ77_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9452819.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21333..22115)
                     /locus_tag="IWJ77_001189"
     CDS             complement(21333..22115)
                     /locus_tag="IWJ77_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9452820.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22108..23025)
                     /locus_tag="IWJ77_001190"
     CDS             complement(22108..23025)
                     /locus_tag="IWJ77_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9452821.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23189..23512)
                     /locus_tag="IWJ77_001191"
     CDS             complement(23189..23512)
                     /locus_tag="IWJ77_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9452822.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24300..27527
                     /gene="fss3"
                     /locus_tag="IWJ77_001192"
     CDS             24300..27527
                     /gene="fss3"
                     /locus_tag="IWJ77_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9452823.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27640..27954
                     /locus_tag="IWJ77_001193"
     CDS             27640..27954
                     /locus_tag="IWJ77_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9452824.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            27967..28341
                     /locus_tag="IWJ77_001194"
     CDS             27967..28341
                     /locus_tag="IWJ77_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9452825.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28351..28686
                     /locus_tag="IWJ77_001195"
     CDS             28351..28686
                     /locus_tag="IWJ77_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9452826.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28769..30109
                     /locus_tag="IWJ77_001196"
     CDS             28769..30109
                     /locus_tag="IWJ77_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9452827.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30187..30861
                     /locus_tag="IWJ77_001197"
     CDS             30187..30861
                     /locus_tag="IWJ77_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9452828.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31094..31528
                     /gene="dcm"
                     /locus_tag="IWJ77_001198"
     CDS             31094..31528
                     /gene="dcm"
                     /locus_tag="IWJ77_001198"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9452829.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            31529..32236
                     /locus_tag="IWJ77_001199"
     CDS             31529..32236
                     /locus_tag="IWJ77_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9452830.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            32226..32516
                     /locus_tag="IWJ77_001200"
     CDS             32226..32516
                     /locus_tag="IWJ77_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452831.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32775..33959
                     /locus_tag="IWJ77_001201"
     CDS             32775..33959
                     /locus_tag="IWJ77_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9452832.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            33974..34093
                     /locus_tag="IWJ77_001202"
     CDS             33974..34093
                     /locus_tag="IWJ77_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9452833.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT        10612 a         6582 c         6228 g        10695 t
ORIGIN      
        1 cttgagtatt ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt
       61 gtccgtcatt tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat
      121 tataaggtag tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc
      181 atagaaggaa aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca
      241 ataaaaaaat ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc
      301 tctcttgtac gggagctttt aacagagatt cttatagaac atccgcaatt tatcgaacgc
      361 ttcaaagtta agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc
      421 cataaatacg atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg
      481 gagatgcgaa agttcatcca agaagcagtt gaatcgctca ttcagcaaaa tgcctatctt
      541 cccgcattcg aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct
      601 gaggatgaac gaacccttct tctgatagag gactgttatt atttatggaa agaattatta
      661 gcagaagcac ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat
      721 catactgacg cctcttattc aaaacgctac tccataaaaa tcctgaaaga agactttcgg
      781 gaaaaagaat tttctaacca aaagaaaaaa atagataaaa aaactaaaga aacggtaaag
      841 aaagacgatt tcaatgaaaa atagcaagta aacgataaca accatcctaa atccacaaga
      901 aatacagcag accttgcttt aaaaacacga atcatgcaaa ggatgaagta aagaagactt
      961 aacgcgtacc caatattagt catttgacta ttaggtacgc gttttgtttt ataagtcatg
     1021 acaaatttcc ttcgttgctg ggtagatttt ccggcattgt tgatagattt tatgatactt
     1081 ttctacattt tgtaagagag gttccactgc atctgtatat tggataaaat tctgacaaca
     1141 actttcaggt gtgtcgaacc actctaatct ttaaagataa atattaaaat aacgattaat
     1201 aatattaaca gtctttattt ttcaccacca aaaacctatc tttgcatata ctaaatagac
     1261 gtatatacac agataggttt gtcttgtgaa aaataatgat tatccttacc aaaaaatgtt
     1321 tgtttttcta catgttcgta cttatcaaaa aatatgatcc ttattgaaat tactggctat
     1381 ttatattcca acgacttctt gtgttaattt ttgttatctc atagtaaatt atgtttcatt
     1441 ttcacttaat ttagttgtca cgtgttcgtc aattcgtaaa aacggtaaat atataaacac
     1501 acttgcggct ataattaata tttgtaaaac agctgctcgc cagtctccgg ctgttgctaa
     1561 aaatcccgat ataactggtg gcgtagtcca aggaatttgc actactacat gattaattaa
     1621 tcccacagca gttgcaaaat atgctactgt caaagaaaaa atcggtgcaa gcacaaaggg
     1681 aataatcaga ctgatattaa atacaatagg caatccaaaa ataataggtt cgcttatatt
     1741 gaatatagcg ggagttaccg ataatttagc aaagtcctta taatcttttc tacgactgaa
     1801 gataaatatt gcaatcaata aagcaatcgt atttcccgat ccacccatta ccgcaaaggt
     1861 atctttaaag gccatattga taatatgtgg aatttgctct ttgtttgcat aagctgccat
     1921 attttcttgc atattctgaa tcaaaaacgg atctaacaat gaacctgata ttactgcttg
     1981 atggatacca aatccaaaaa atagattagc aatagatgta attaacaaaa atcctggtaa
     2041 accagttgta attttactta atggctctgt aactatcgtg gtaattagct tatttatatc
     2101 catattaaac aattgaatga ttataaatga caataaagca aacgaggtaa tcgtaataat
     2161 gattgggatt aatacattaa aagactttaa aacagcggga ggaatatttc cggatagctg
     2221 aatctgtagt ttcttattat tagaaagttt aataaataat tctgtagccg ctaatccaac
     2281 aaatatacct acaaacattc ctgaagctcc tgtataggat ataggtatca cacccgtcac
     2341 ttcaacagct ttatccgcat tttcaggagt aaacatcatc tgcataggtg ttaatacaac
     2401 aaatgtcgaa atagcaacta ataccgctgc aatatgattt gtataatttc tgcttgcaca
     2461 catatgataa gatactgctg ctacaactaa taatgtagtt atgcttaatg taccattggc
     2521 aattgaagta ccaatttgtt gtaacgtggt caatgtacta gtatcgacaa tttcccccat
     2581 gaatcctgtt ggttctaaga tcactgaatt aataagtgtc acaaatcctg ctaacatcat
     2641 aaatggcata aatgtagcaa acgcatctcg caaagttctt aaatggatct gattccccaa
     2701 cttagtcgca actataccaa atttttcaaa tacaatgtcc ttcctgctac ttttgcccat
     2761 attttttctc ctttatgctt attgtaatcg attacttttt ccacccaaat aatacatcac
     2821 ttatcaaaaa aagtaaatac ttttaattaa atatatatat cttttttagt ataaaaaaat
     2881 aagctctgaa aaaataattt tcccgagctt atttttcaac aaaaacagct tcaaaaacaa
     2941 agtcttgata taaatgtctc gataccgaat actcgaaagg tttaccagac gatagagcaa
     3001 caactttgtc tatctcaatc ataaattcac ctggatctat ttttaatatt tttgcgtcaa
     3061 attcattcgc agtatctcct ttgatacgta tgtgcgaagt ctttatttcc agacccagtt
     3121 catttgtgat ataagcatat acagatttct ttaaatgttt tggttccaag cctggtatga
     3181 ctgctaatgg catgaatgtt tgctcaatcg agtatggttg acaatcaata aaacggcttc
     3241 gtacaatatt atatacatag tcttttacac tgatacttaa tttatcagct acttttttcg
     3301 atggaagttc aattgagaaa gtatggattt ctgaatctac gtgttcttca ccaaccacgc
     3361 taaacaaacc ttcgtttgga ccaagaactc gtgagttatc aattggtgtt ctgacgactt
     3421 cagtcccata cccacttcta cttcgcaaaa ctccttcgga aacaagtaag tctacgcctt
     3481 tctttactgt taacttgctt gtatgataaa taactgccaa ttcatctcct gtaggaagtt
     3541 tctcaccaat tttatactct ccatttacaa tttttttctt tatatctaag taaatttcca
     3601 tatacttcgc aatttttttc aatggtgacc ctccaatcaa aaacatatct tcatctatac
     3661 tttaacataa ataaaccgat aaaatccaca tcaaaattat acacacaata aaaaagatat
     3721 atatatttaa acaaaactat ttactttaag ttgtaagcgg tgttatcatt aagtggaaac
     3781 aggaggtaaa aaaattatga caactatgaa aaagctaact tttccagatg atttttggtg
     3841 gggttccgct tggtcagctg agcaagcaga aggaaaagga gaaacaggaa aagcagagac
     3901 tatctgggat cgctggttta aagaacaacc ttaccggttt tatcaacgaa tcgattcctc
     3961 tattacaacc gatcacatcc atcaatataa agaagatata gaactaatga aaaaaactgg
     4021 gcataactcc tttcgcgttt ctatttcttg ggcacgaatg tttccaaatg gcggtaccgg
     4081 aaaagtaaat cccaaagcca tttctttcta tcgaaaatta ttttccgaaa tgaataataa
     4141 cggaatcaaa gtatttgcca acttatatca ttttgacatg cctgctactc ttcaagataa
     4201 aggaggatgg gaatcacgtg aagtagttga tgcttatgtt cattacgctg aaacttgttt
     4261 taaagaattt ggtgacttag tttgtcattg gtttaccttc aatgaaccac ttgggcctat
     4321 tttaggtact tatcttgaag attttcatta tccaaacctc attgacttta aacgcggagc
     4381 gcaagcagca ttcttcacta tacttgcaca cgcaaaagca atacaagtat ttaaagaact
     4441 agctcttcct agtaaaatag gcgttattct aaatttgagt cctacttttc ctcgtagtca
     4501 aaatcctgct gatgtgaaag cagcagaaat cgctgattta ttttatacgc ggagcttttt
     4561 agatcctatg gttaaaggga atttttctaa aaagcttatc ctgttattac aagaatataa
     4621 tcagcttcct gaagattata cgaaagaaga tcttgtatgt atctacgaaa ataccgctca
     4681 aattcttgga ctaaactatt acgagccacg tcgcgtcaaa tctcgttctt ctatggtgga
     4741 tacatctagt ccatttttac cagagtggtt tttcgaacca catatcatgc ctggacgacg
     4801 aatgaacgaa taccggggat gggaaattta cgaaaaagga atttatgatt tgtgcatgga
     4861 tatcaagaac cattatggaa acatcgaagc atttatctcg gagaacggaa tgggcgttgc
     4921 ggatgaagaa cgtttcatgg atgatacagg gcaaataatt gatgattatc ggattgagta
     4981 tatcaaagat catcttgctt acttatggaa ggcaataaat gaaggatgta acattaaggg
     5041 gtatcatctt tggacattta ttgattgctg gtcatggatc aacgcctata aaaaccgata
     5101 tgggctggtt tcactcgacc ttactactca aaaaagaaca attaaaaaaa gtggcgaatt
     5161 ttacaagcaa ttaagtgata caaatggatt tagatacgat gtagacttgt tagttgaata
     5221 atattctaaa attccacttg aagtgaacac attagaaaac aagaataatg agaaatacca
     5281 ataacaaata ctttttcttc atacttcatt tatacttgtc agtataagag actgtgacaa
     5341 atgtagtgat cctcagaagt taggtctgga aatctaactt gagaagattt ttttaaacta
     5401 cgtccagtct tttttatttg tttaaaattg aagttgacaa gtcttcatta aaaatttaga
     5461 atattcacaa gtttgacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat
     5521 tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc gtttttggtt
     5581 atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccgggttt catccgctgg
     5641 tcgtcacgtt gatgtccctg ctgatctatg ctggttctgc ccaatttatc acggtcagca
     5701 tgcttgctag ccatagtcca ctgctctcca tcgttctctc gacctttcta gtcaattccc
     5761 gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa
     5821 atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga
     5881 actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt
     5941 tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga
     6001 aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag
     6061 tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga
     6121 ttctggtata tgtcggactg atttttatcc caagtaactt agtgatttta gttgtaacac
     6181 tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg
     6241 actattttag gttgtacttt agcaacttgg ctttctagga tcctaccatt tgtcttactg
     6301 aaaaaattcg atttgccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt
     6361 atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc
     6421 aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc
     6481 ttattggtga tcgtcttggt aggaattctt tccctttcgt tgattcgctt attataaata
     6541 aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat
     6601 aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc
     6661 ctaatatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc
     6721 catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa
     6781 tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca
     6841 tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt
     6901 tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgtttg atttgatcaa
     6961 agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac
     7021 gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca
     7081 gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta
     7141 gttgattcaa aatcgaaccg acgcccccag ctccgttgat cactaagatt tttttcccgc
     7201 ggttggcatt ttcttctgga gtcagaccaa atttttcaaa taacaattca taagcggtca
     7261 atgaggtcaa cggcaaggct gcggcttctt catctgaaag attttttggt gcaagagcga
     7321 caatacgttc gtcaaccagt tggtattctt gattgcttcc tgctcttgtc gttgtacctg
     7381 cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc atcacctaca gcaacaactt
     7441 tcccgactcc atcaaaccct aagatacgta aagcatttct tatcccgtta tcttggcgaa
     7501 gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac
     7561 tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg atcgacaacc
     7621 cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc
     7681 ttcatcaagt tggagcatcc atacagtttt catttcaata tatatagtat gctatttgtt
     7741 ccacttttat tcatcataat tattctctta cttaaagtaa actatttgca cttttttggt
     7801 taaaaaagag gttgcgacaa gacttttgtc acaacctctt ttaaacggtg ttcaatcaac
     7861 tgaccctcgg tattcgctca aaaaacgaca aaatatgagg agctattttt gtttggctgt
     7921 tcttattact tgatgcagga cagctttttc acgacctcta attcacggtg ttcaagcaag
     7981 aaatcattgt tcactgaaaa gcgttggatg ttctttttca aaggtgtcta agatctctcg
     8041 tacttcatca gtagatttcg tattcatcaa ctgaactcgc aagtcatttg ctcctggaaa
     8101 tcctttgata tagattttaa agaaacgatg tagtcctgtg atggaacgcg ggatgatttc
     8161 tgaataatga tcttgcagat ctagttgcag acgaagtagt ccgatcagtt ctttttctgt
     8221 atgttccctc ggttcttttt caaatgcata aggattcttg aaaatccccc ggccaatcat
     8281 gatgccatct acgccatatt tttcggccaa ttcttctcct gtctggcgat ctgggatatc
     8341 tccgttgatc gtgatcagtg tttgcggagc gatttggtca cgtaaagcaa tgattttagg
     8401 gatgacttcc caatgtgcat ctactttact catctcttta cgcgtacgca aatggacaga
     8461 aagattggct atatcttgat gaagtaggtg actgatccat tcttccatct cagacatttc
     8521 cgtatagccg atacgcgtct tgacgctgac aggcaaaccg ccagctttag ccgcttcaat
     8581 caattgtgcc gctacttctg ggcgaaggat caagccactg ccttttccac gttctgctac
     8641 attagggact gggcagccca tgttgatatc gattccttgg aagcccattt ctgccatagc
     8701 aatgctcatt tctctaaaaa attctggctt gtctccccaa atatgggcaa ccatcggctg
     8761 ttcatcttct gtaaaggtca atcttcctcg tacactgtct tttccatctg gatgacagaa
     8821 actgtctgag tttgtaaact cagtaaagaa gacatccggt gctgctgcat gctttacgac
     8881 atggcgaaat acaacatctg ttacatcttc cattggtgct aaaacaaaaa aaggctttgg
     8941 cagctctttc caaaaattag tagtcattta tttgttactc tccattctat caagcagtga
     9001 agtcgcaatc tttctcaaaa acattgtcat tatactagga aacaaaaaaa gaagcaaagt
     9061 gaacagctta aaagatgttt ctgattgagc aaaaaaagac tgtgacaaaa atcttatcac
     9121 aattcctttt ccggaataaa tggtgttcaa aagcttgcat ctgcggtaat aagtccaacc
     9181 aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga tgcagaacag
     9241 ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa
     9301 caaacgacaa aatatgagga actattttcc cgtttgtttc tctaatactc aggtcaaaac
     9361 gctgatttca caacctcttt tctaacttct caatcctctt cctttattga tcaaatacca
     9421 agtaacggcg tacaaaacaa cgatgaatag cacaagtacg atcatcgatg ttgctactgg
     9481 cacatcgatt gttccaagga atccgtaacg gaaaccggag atcatataaa cgatcggatt
     9541 gactttcgaa acagcttgcc aaaatggcgg caacatagag atggcataga aaacgccacc
     9601 tagataagtt aacggctgca agacaaatgt aggcacgatc gatacatcgt cataagactg
     9661 agcaaaaatc ccgttgagca gtcctgctaa cgaaaacaag attgccgtca tcaataatgt
     9721 aatgatgacc atgaaccatg aatacacatg aagtggtaca aagaacaagg aaatgatcgt
     9781 tacgagagag ccgacgagaa tactgcgacc cactccgcca atcacaaagc cccaaatgat
     9841 cacatgcgta ggaacaggcg ctaccaagat ttcttcgatg tttttctgga atttttgaga
     9901 gaagaaggac gaagaaacgt tggcatacga actagtgata gcagacatca taatcaaacc
     9961 aggcacgatg aattccatat atgaaaaacc acccatgtca ccgatccgac caccgatcat
    10021 ttttccgaaa ataacaaagt acaaggatgt cgtgatcaca ggcggaacca gtgtttggac
    10081 ccagatacgc aaatagcggt tcgtttcctt gatagctaaa ctttttaatg cagtaaaata
    10141 aagattaaac atttttctcc tccgtatgat tattttcttc tgtgattttc aaaaacagtt
    10201 cttctaaacg attcgactta ttcctcatag aaagaacttt gattccttgt cttgtcagct
    10261 gatcgaagat tccgttcacc ccttgatttc gttcaacttc gacagcgatt gtctgttcat
    10321 cttcaaatgt acaagcgtat ccttcaatca caggtttggt ctcatatggc gctaaatcaa
    10381 agataaatgt ttcaaattgc agtttggata gaagctcttt catactagta ttctcgatca
    10441 gttcacctga ttggatgatc ccgatgttgc gacaaagcat ttcagcttct tctaaataat
    10501 gcgtcgtcaa aatgatcgtt gtaccatttt cgttcaattc tcggagaaac tcccacatct
    10561 cgcgtctcaa ttcaatatcg actcctgctg ttggttcatc caagatcagc aaacgtggtt
    10621 catgcatcaa tgccctagca atcatcagtc ttcgcttcat tcccccggaa agcatacgtg
    10681 cacggacatt tctcttttcc caaagattgg actgcttcaa atatttttcg ctgcgtttca
    10741 tcgcttcttt gcgagagaca ccgtagtagc ctgcttgatt cacgacgatt tgctggaccg
    10801 tttcaaaggg attgaaattg aactcttgcg ggactaatcc aatctgttgc tttgctcgga
    10861 ctagatccgt atccagatca taaccgaaga cattcacttt tccagatgtt ttattgacta
    10921 aagaagtaat gattccgatc gttgtagact tcccagctcc atttggtcct aatagcgcat
    10981 agaaatctcc ttctttcacg gaaagatcga ttcctcgcaa tgcttcgacc ccacttccat
    11041 aaaccttttt caaattattt atttcaagcg catttgccat ttatttccta tctccttata
    11101 gacatgattc tttattgtat catggaatac agcagatgaa aaaacaactt ggttccagcg
    11161 aaaaatcagg ttgctgggct caggaaaatc tcctaatgaa agacatcaat cggacgaagt
    11221 cagacaacct tttcagaaaa aacaaatcga agataaagga ttactattca tatgtgcaac
    11281 aaaaggagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
    11341 cggcggaagc aaaagccaag atttttgatc caataaatac tttcgatcac ttcgcctatc
    11401 ccaatacatg ccactgccgc ttttcatcaa ataacgcggc ttccgttttt gcggtttcta
    11461 ctaaagtctc tgggtaatcc aaaactttca gcaaggcaat cgcatttcgg gtccttgaag
    11521 gtccttcttt taatttgaaa tcaaaactga ttccttttcc ttctgttact tgttcttcaa
    11581 agtggacatt ctcacaataa ttctttaaaa tctctgtcaa ttcgatatca tgtgtggcga
    11641 caaatgctaa agatggatac tccgcaagcc accgaacgac tgaagcggaa gaagcaatcc
    11701 gttcgatcgt atttgtccct tttaaaatct catctacgaa gcagtagcag cgttctttgg
    11761 aagccacttg gtccaacaaa cgtttgatcg atttgatttc tgaaacaaaa taactgtcgc
    11821 cttcaaataa atcatcttcg attgccatgg aagtcaacac atgtccaggt tgcagggtaa
    11881 actgttctgc aatcgctgtt tggatcgtct gtgctagaat acagctgatg gctatacttt
    11941 tgacatacgt agattttcct gatgcattgg aacccgtgac taatgtattc ttctgccagt
    12001 tgaccggatt cactaccgct tcttttaaca atggatgata gatatctttc gctgtcacac
    12061 cgccctcagt aaactctggt cggcaagtga tgggcatata agtacggtaa ttcaatattg
    12121 cagcggctac ttctaattct cccagcaatt cccacagctt gatggcttca gttgaatgct
    12181 tttctaattt agtgagaacg aaatgatagg agataaatgg cagcatgaac atgatgttga
    12241 catattcaaa aatcaactct gcttcactcc cactcttcat acgaaatgaa aaacctaatt
    12301 tagggatgtc tcttaatggt ttagtttgct gtttcagctc ttcttgaagt ggtgtcggca
    12361 gttttgctag ctgattggcg gctgaaatcg ttgaaaccaa ataactcatg ctattcagct
    12421 ctgtttctag tgtttgtttc ttcactagat aatagaccgt attgaatact aacccaccga
    12481 ttgccaaata gattcctagt acttgtccta aaagaagcaa acccaaaccc accagcggaa
    12541 gcaagccaag aaaaataaat aaacctacgc gtccaatctt tttacttttt ccatccgcta
    12601 gatattgttt tgcaaaatta tgatcttttt ttcctaaacg agcgaattga tattggattt
    12661 tttctctcag ttgaggattt tcctgataaa aagcaatcag tttttctaac tgctgatctt
    12721 caccaaaatc aaagctgcgt aaacgttgat acaacgcttc tgaacctaca ctagaatagg
    12781 tactgttgat tccttcgaat acttcgaaca tatccaggtc ataccaagta agatcatcga
    12841 tttcgctatc ccatgaacgg aacttcttct ccgtgagcca tgcttccttc aagctttctt
    12901 ctttatcaaa tctcggttga taaggaattt tttcccattt gctacgaact gtttccttta
    12961 atttcaaacg tgaaaaaatg tctaaagcaa ctaataaaat aacagctgca ataataatta
    13021 aaacgataaa ttctctttga cccatatccc tctccgttcc tttatttgtt agctttagta
    13081 tagcactgtt gcgctatttg aaaagagggt aaaaaaacac ctttcaaaat aaaaggtgtt
    13141 tttttcttgt atattacata tttatctctc atttatcaaa attgacaaat aatcataggg
    13201 aggaattgtt gttaattctt gtttattctc cagtgcattg acaactgtcc caagggaaag
    13261 gatagcctca ggtactattt tttcttgttc tgttggatta gctaagcgaa gaacaaatcc
    13321 ttgttcatga aaataagaag gataaatcga tgatacaatt aagtcttcag gtaattcaat
    13381 caatgaaaat ttacgaggaa catcaagttc attttgtaat ggccaaatct tgttatctaa
    13441 cctgtgaata aatacattca gtttctgctt ttgataagaa atagattgtt ctaaccgttt
    13501 ttctgcctgt tttgccactt tgaactcatt aaatcttcct tcttctacat taaatccaaa
    13561 agtgacttta tactcaccta tctcttgagc tagcggtgtt tccatcatga catgcccttc
    13621 atttgtcgta tctcctgaag ctcgcccagg tcgccaagca agattaggtt ttccaagttc
    13681 ccctgttgtt gaaaataagg ttatgattaa ttgattttct atacgttcat actcttttaa
    13741 accatctgca taaaacgtaa aataatgatc attttctgcc acagagacac tcttatcgaa
    13801 tatttctaaa ttaacaggtt tctctaccat agtttctgac caattcggag gaatgacatc
    13861 aggggtattt tcaataaatc cgttttgaat ttgtgcaatt gatttttcag atttatctaa
    13921 aacattaatt gctaagcgca tacgatgcga gtaaacagta ttatgaattt tgagtgtgcc
    13981 ttcaacgatt tgactttctc ccagactgaa cgatatttca taagatattt cttccatttc
    14041 gggtttttca gataaacgat cttttaaatc tttaggaagt tgagcttttc cgtaaacaac
    14101 taatgtttct tgtaaagaat ctttatatac tttagctgtt tctaatttta gagatagttc
    14161 tgtatctcct tctaatggtg aataatcata cgtatcgcca tcatttgctg aatcgatcaa
    14221 atgaacgaaa tcaagatatt gtctatctcc aactatcaaa ttcaattgcc catctttaaa
    14281 agaaagttta tatttattgt tacttatctc agttttgtct atctcgattg ctgtttctaa
    14341 tttttcatta tttttctcaa attcaacaac agtgtatcct agtgctggca catttacatt
    14401 tatttttatg tcaagttcat aataagctgg ttcatcaaaa tattcgaaac ctgtagcaat
    14461 catccgataa gcatttttcc gttctggaaa ataaacttct ttttcaatga tattataggg
    14521 actacctttg aagtgtattt tctttgaatc agctactaca tgcacgattt tcgatccact
    14581 ataattctca gtcgttgtgt tgaaaacaag gacttcattc gattttagct ttaatgcatc
    14641 agccatccgt tttacaataa ggttttcgat tccgtcagct atctcattag cttctttgat
    14701 tcgatggaaa atatcttccg caacattatc tgaaacacag ccaccaatac tatcatgagc
    14761 ctggttttgc aaaatctttt tccataaccg acgaagtagg cctttactga tatggatacc
    14821 atttctttct gcaatcagca tgagcggctc aactcgtttt agaatttttt gttccaattt
    14881 aaaattttct attttcattt ttgttcttac tgagccaata gaacgatgaa cacgagagta
    14941 aaccggctga cgaagttcac ctttatattc aggtaattct tttttgtttc tgataacttc
    15001 tacataagaa ggataatctg aaataatatt gtggaattta ctgtatttat tcaacttctc
    15061 tacgacttga tcaaaattta aaatcattga cttttgatct acacccgaag gtactaaaat
    15121 atctttgttt tttccttgca tatgaagcat ttcaactgct tcatccaact tttcttttgc
    15181 aaattcagga atatctttaa ttgcgtctaa ggtcatcatt ccagtcatat aaccaaatgg
    15241 aaaattcatg gcataaacaa atttatctcc caatcctttc cagataaaat aaggagatgg
    15301 aaccatcgtt tcaaagttta atcctcgcca acacatgaaa ttatcgattt ctacttgatg
    15361 caaaagtgta ggcaaattcg cattgaaccc aaacgtgtca ggcaaatacc ctaccatcat
    15421 tggatctcca tatttaagga tcgtatcact gattccaatc attaagtttc ttaaaattga
    15481 ttcagcatcc actaataagg catctgtttg tgcataccac ggtcccacga atagttgtcc
    15541 tgttttcatc agtcgttgga taaccttaag cttttgcgga ttgatctcca aatactcatc
    15601 gacgattgaa gtttgtccat ctaaacaaaa gtttacttta gggttccttt ccaattcatc
    15661 tagaacttca gtaaagacct gatcagacaa tacgatggca tcttcatttg taaaatacca
    15721 ttctctgtcc cagtgcgtgt gttgaattat atgtgcattt accatatcag attctcctac
    15781 tttccttctt ctactgaatt tacttgttgt tcttttttat acttgatgga ttttagtgca
    15841 ttgaccatta acgctgtaac cactccccca ataacaattg atactaaata taaaggtata
    15901 ttcgtaacaa aaggaattgc cataaaggtt gaaagcattg tttcttgagt aacaccaaaa
    15961 aatccattta gaccacatgc tactgcagtg cccaccacga tgcttggtat tactcgtaaa
    16021 ggatctttca cggcaaacgg gatcgtccct tcagtaatcc ccacaaggcc cataatcata
    16081 gctgtctttg cattatctcg ttccgattca tcataaagtt ttttgtttaa aattgttgct
    16141 aaaaacattc caaatggcgg aatagcaatt cctaataaca tggctgtact aggtccaaag
    16201 cttccgcttg aaaaagaagc aaccccaaaa gcatatgcga ttttattgat aggtcctccc
    16261 atatcaaacg ccatcattcc tcctaaaata gccgacatca aagcagcatt tgctcctgac
    16321 atgccttcta gccaattagt cagtcctgtt gtcattgctt gaataggcgt agctagtaca
    16381 taccataata tgattccaac aactaatgta gtaacaaaag gaatcagcat aattgatttt
    16441 agactaataa cttgcatcgg tacttttact tttttcaatg cattagctaa atagccacaa
    16501 gctaatccag caaccattcc tccaataaat cccataccaa gattttgaca catcatccca
    16561 gtgataaaag ctggtgctag accgggacgg tcagcaattg aatatgcgat ataggctgaa
    16621 atcatcggta cgataaactg taatcccgtc tgaccaatca ttcgcaggac tccccagaat
    16681 gtatccgtct ggtcccaaac acctgtgcca ccaaaaatga ctgcaataga agttagtaac
    16741 cctgctgcta cgacaagcgg gatcatatag gaaaccccac tttggaaatg atttagaatt
    16801 tctcttcctt gtttcttgaa ttccattagt aactccccca cgatatattt tttatttgtt
    16861 aattaatgca attgcttcat caatcgtctc ttcaccatct tttactacct ctgaggtccc
    16921 tactgttaaa atcggtttac cgtcaaagcg acctcgttct gaaacatttg tatctactgc
    16981 aaatatgaca acatctgcct cggaaacatc cgaaattgaa agtttgtttt ctatccccat
    17041 agccccctgt gtttctactt taatatctaa ccctttcttc ttcgcaaact tttctaaatt
    17101 agcttttgcc atataagtat gggcaatccc tgcgatacat gctgttactg ctactatttt
    17161 tgtcatatga aattcctcct tttaaaatga tagagctttt accaattctt ttttatttct
    17221 cgaatctttt attcttttaa caaactcgtc atccatcaat cttccagata attctgctaa
    17281 aatacgtaaa tgattagtac ctttatcagt gtcttttatt gctaacaaaa agacgagatc
    17341 aactggttga tcatctaatg cgccccattc aacagaccgc tttaacttgg ctacagccac
    17401 tgttgattca attactgcta ggcttttgcc atgaggaatg gcgatatttt tcccaattcc
    17461 tgtggtcata tggttttctc tgtcaaatac gtctttttga aattgtttgt ggtcagacac
    17521 aaacccatta gtctctaaca atttcgataa tttttcgact acactttttt tatcagatgc
    17581 atttaaatct atcactatat tatttatatt gattttttca ctgatcattt ataagccttc
    17641 tttcaatata taaactaatt tttcaaacga cgaactcgtt tttaaatttg ctaataactc
    17701 atgattcgaa gcgatttcat tgaatgtttc ataaatttta ttcaattcag gttttacttt
    17761 ttcattaagt gcaaaaaaga aaacagaatg aacaagtgtc ccatcccaat caattccttc
    17821 tggattaacg tagacacaga ttactggctt ttttataaaa tctacttcga tatgcggcat
    17881 agcaaaatca ttcatcgctg ttgaagatag tttttctctt ttgattgctc cctctagtat
    17941 cccttctttc gcgtaatttt cttttaccaa ttttttggta attatattta aaacgttcac
    18001 ttgattatcc tttatttggt gggtaaaaaa gcaaaatcgt ttatctaata aatttagaaa
    18061 agcatcttct gtttgttcat ttaactgtag atactgtttt acttttctga catctgcccc
    18121 taacatcaaa ggactaacaa caataacagg aaaatttgta ttttcaatcg gaatcgtgct
    18181 tataacaagt tcatcagtga cttttctatc ttgtagttca ttgatgctta gtacatcttc
    18241 tatctcaacc atatctgcaa aaccgttatt tattctttgc tctaataatt ttgatgttcc
    18301 atatccgcta ctacaaacta atatcacttt cgttttgtca ggtttgtaac gatccaaaag
    18361 tgattgaata tggagtgtta cgtaagcgat ttcatcttca ttaaaatgta tacagaatct
    18421 ctcttcgatt tcctctaata aatctacact tatcataaat gaccttttaa aagaatattt
    18481 gattttgtcc gtataagggt tatagatatt tactcccaat ttgagtcttt taactgctgc
    18541 attgagatgt aatactagat tattgatcaa ttcttcatca ggataaaatt catttaattt
    18601 aattcttact aactcttgta gctcaacacc catatctaaa taggaaagct tcgtttgatc
    18661 taagtcgtga ttcatagcat ttttttcaat cgaagtaatg tgaatgtcaa cataagcttg
    18721 ttcatctatt ggcacaataa ttgaaaatgc tttttcaatc attttgatta aaataagtgt
    18781 attctttgag atttcttctt cagaattatt actacgtgat aagaaattgc ttgtttcaat
    18841 cctttctatc gcaatagcaa ggtggatagc taaagtttga aattcataat ctgtaaattt
    18901 caattgtgtc ttattcatgt atcgattaag caattcggct acttttttca atatgtcctt
    18961 atttaagaaa ttattcgctt caccaatgaa attgatattc aagtctaaga gatctgtttt
    19021 actgttagat gctgtaaagc caccccaata atagttcaca acttctgaca tcaaccttct
    19081 tttacttttt tcatcagctt tgatgcgcac gccatttcga tctgaaacta aggacatccc
    19141 atgcttgttt agtctttttc taacatcttt cattgtgctt tctaccgtcg acctgctcac
    19201 gtataattcg tcagcaagat tttgaatctt tagagaatca ttagtattca ataatttagc
    19261 aaacacataa gttagacggt cctccggaga gtctgcgata ttgattttat gactggataa
    19321 taaatttcga agtgtttctt tttttccaat aagcgaaacg ccttttttag gttgaaccaa
    19381 catttgtaca tctggcattt tttggattat ttctttcaaa attaaaatat ctttgctaat
    19441 tgttttcgta gatacatata attgttctga taattctttg taagttgtgt gctcattttc
    19501 aatcaaaaag tatagtattt tcatttgccg ttcgctaaat tcgatgccca ttatcttcac
    19561 ctcctgaata cagcttaagt gtacaattat tatctctatt ttcatacaga agaaagttct
    19621 tgattaatgt agaagtttct aatattcttg attatttcaa atcaataaaa aagcctgcaa
    19681 tatttatatc acaggctttt ttctctatta aatatatata agataataca tttttctact
    19741 agttctcact aatcttgtat aattaaaccc tccgcaactt gacggagatg gagaagatgc
    19801 ccccgtctaa tctagcggtc atctctcctc ccattccttc caccaatttt tttgacaggt
    19861 aaagcccgat tccgctagat tccgtatttg tgcggctggt attttcggta taaaaacgct
    19921 ggaagacttt ttcaacagag gtttgcgttc ctggtttgat aggattggcc actgttaatc
    19981 gaatgaatgg atcctctttt gaaaatgtca cctttgcttg tccatctgca taacgcaata
    20041 aattctgtat gatattttgc agcactcgca gcaacatgag cggatctgct aatacagttg
    20101 tatttatcga gtgttcagcg aacgctacct cgatattttt ctcagaaaat tgttcataaa
    20161 aagagaaaaa tacttcttcc acctctttag aaaaagaaag cgtatggaga tccatttctt
    20221 tttgcttcgt ctctattcga gcaagatcat aaaactgatc cgtcatactg agtaatcttg
    20281 caacagattg tttgatgatc gttaaatact tctcccgttt ctccgtatcc tctacctgtt
    20341 ccagtaattg tacataaccg ttaatagcag tcaaaggagt tctgagatca tgagaaaggc
    20401 cgacgatcga tagtctgatg ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat
    20461 tttcatccaa ccatgtattc agtgcatcta ctaggtccat tagattcttt tctcgaaatt
    20521 cgactgaaag acgaccacca tattcttgtc gttcagaaag agtggcgatt ttcttagtta
    20581 atctcttgat ttccctttgc aggaagaaaa taaaacttcc cataaagata acaacgagaa
    20641 gcaacagcca tttaatcaaa tctaaacccc actccccaca ccgtatgaat atacacagca
    20701 tgtgcctttg ctagcttccc tcgaagattg gaaatatgca tattcactgt tttctcacta
    20761 tcaaaatatg gttcttgcca aactgtttca taaagatttg ctcgcgaaaa tacttttttc
    20821 ggatgttcta aaaaaagctt cagcagatcg aattctcttc ctgtcaaatg gacagccaca
    20881 ccattcacat ttacatccct cgactctttg tccagtaaga tttccttgta atagatgcgt
    20941 tgcgacagat cttttacttg gacagaagtc tgatgacgaa gttggatgcc gattcgtaac
    21001 aaaagttctt cgatatcaaa tggtttcgtc acatagtcat ccgctcctgc tgccaacaag
    21061 gaaactttat ctctttgatc gctttttgct gaaagaacga tgatcggaac attcgatgtc
    21121 tgtcggattt ctgctaacaa tgcttcacct gaaagccctg gaagcatgag atctagaagg
    21181 atcaaatcga caggatatgt gcttaacaat aattttcctt ccgtacctga ataagcatca
    21241 agaagtttgt atctctgttc aagaacctcc tccagtaagg aatgaacagc ttcatcgtct
    21301 tcgataatca aaatagtttc cagcaggacc acctacttaa tgtcagattt ttggaaaata
    21361 aacattccta gagctgatga taccacgatt gttactagac tgaccagaat cattttcggt
    21421 gcttgcgaat aagttgcttg ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac
    21481 cactccgtta gaaattccaa gcctttgatt tttgcgaaaa caccgggaat gaaggccgtt
    21541 cccatgatat aaagaaaaga aagaagcaaa ctgacactgg tacttctagt gatgaatgtc
    21601 aaagccactg gaacagataa tgtccctaat gtcagcagaa gcaatacagg aaatactttt
    21661 agaacataat ttccatagtt tttgattccc tcaacttcac caaatccgaa agcaagtgtg
    21721 taagcaagca tggctagtcc cgtgatcaaa accacgccaa gtgttgtgat caaaagcgta
    21781 acgagaagtt tcgacacata ataagtcatt cgattccgtc ctaaagataa cgtattgcga
    21841 atcgtaccgt tttgaaattc ttccccaata taaaagcacg caaatgctgc gaaagccaga
    21901 agaatccatg cagccatttt ttctaccatt aaaatccctg ttgcaccgtt gatgactgta
    21961 tccgttgctt ggtttaattc attgccgtat tttgtatgca tataccattc gttccaagaa
    22021 taaaaaagcg gaagtacgac catcagtcct agtgagaccc acattttacg gctcttccat
    22081 acttttattt tttcagcagt taatagatta aacatgtgga ttgcctccag ttaattgtaa
    22141 gaaataatct tccagttttt gcccaactag gtgaatacca tccacaggaa cttttgcttc
    22201 taccaatgct tgattgatgg ctgctacttg gtcaattcct tcaaaaagct ggataacacg
    22261 gctagattgt acaaaataat ccctataacc aagagagtcg agaaccgtta ctgcttgttc
    22321 cgtcgccgaa gtgtcaattt ttataaattg acgagattct tgtgccaatt cttctttcga
    22381 taattctttg atcaagtgtc cttcatgcaa aataccatag tgagtggcga tctgtgacaa
    22441 ttcatctagc aagtggctag aaagcaagac ggtgatgccc cgttctttta ccaaacgttg
    22501 gatgatttct cgcatttctg ccatccctgc tggatccatc ccattcgtcg gttcatccaa
    22561 aatcaaaaat tctggattcg tgatcagtgc atttgcaata gctaaacgct ggcgcatacc
    22621 taaagagaaa ttctttgctt tctttttccc tgtattttcc aatcgcatca ttttcagcag
    22681 gtcttcgatt tctctatccg acacaccgcc gttcgctgct tggatacgta ggttatctct
    22741 tgcagttaat tctggataaa gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc
    22801 tgcctgcaaa tccgtcgttc caaataattc gatcgaacct tcatcaatcg agatcaaacc
    22861 cataatagtt ctcataaagg tagacttacc cgctccattt tccccaatca atccatagat
    22921 cattccacgt ttgatttcga ttgatacttt atctaatgct ttggcagcac cgtatttttt
    22981 ggttatacca gttgctttca agaccgtttc atttccaaaa tccatactta tccttctttc
    23041 ttattgatga gtgaagtata gttgacgggt ataaagaaag cagccagaaa tgtataaaga
    23101 aagtgtaaat tttccaattg aatgtcaaaa aaaacaatcg gacaatacgt gtctttccta
    23161 ttatcaggca gtttgttata cgcttgtatc agtttcctga ttctttcttt tcgaccaaag
    23221 tcgtttctct gtattcctct tcactgaaaa tttttgcttc tttcagatca agcttgagta
    23281 attttaccag accggttata aggataacta tagcaagact ccctcccaga aaaattagac
    23341 tgtctcgcag acgtggttgc gttgtcttcc aatcaaagag cgcaccaaac agtgctaatc
    23401 caagtggact ggccaccatg gtaatcgttg ttatcgttga cataagtcgt ccttgtatat
    23461 gcgttggaac ttctcgctga agccagacca tataaaaagc aaaagaaaac attaatcctt
    23521 aaaaacggac ttatgaaaaa taatacatgc cgtttgggat ttacaattag ctaccaaata
    23581 agaggttatg acaaaagact tttcacaatc ccttcttccg aataaacggt gttctcctgc
    23641 caactcctcg gtcacaagtc acaatattta tcaaacatga gaagcaattt ttgtaaattc
    23701 cttcttattt cttgaagctg accacttctg tcacaacctc agaagcggat aaataatatt
    23761 gtttgatgat actttatctt tttcccgttt gtttatagat cgaatgatat tatagaccaa
    23821 gaatcctacc tattaacata gctaaaaaca caaaaaaggc actttccaaa ttgtggaaag
    23881 tgccagaaaa gttaggatta aggtaactgc gctcagcatt gcttcgccca gtacgattct
    23941 cgtaactcgt gaactcgaac gataatcgca attaacgttt tgagaactgt gaagctttac
    24001 gggctttctt aagacctggt tttttcttcg tatcattgaa taagtttgaa aaaccttgat
    24061 ttataaaggt tttcccgtaa atatatttta ttatatttga ttaaatttga aatgtttgtg
    24121 ttctaaaatg tgttctaatt taaaggcagt atatttcgta ttttagatta aatttttagg
    24181 ctcattcatt ctcaaatcca ttctattcag tcaataaaaa ttttaaaaag actttataaa
    24241 caaagatgtt caagcgagca tcttattttt ttgtgcttaa tttgaaagga gaacatttca
    24301 tgtataaaaa acaaaaaaag aaattatgtg tattaactat tttgagtttg gctgttcaag
    24361 caattgttgg agcaattgct cctacgatta cttttgctga tgaaattacc catccacaaa
    24421 cggtaaccgt agaactagat ttagcacatc aatatgctgt tgaaggaact tttagtgatg
    24481 gtcgtccaat gtcagaagtc actgttccgc attatgcagt gtacaacggc tttaaacaag
    24541 atattttctg tattgaacca ggagtcccga tttacaatga gttcactcct ggctatgaga
    24601 aaaacccgtt accagatatg tccgataaag ccaaattagt ttccgtttta tggaaaaaag
    24661 taggtacaga tgcagatact catattgtgg cacagaagat gatctggcaa gaagttaatg
    24721 ggtataccct tcactcaata aaacgatcag atggtagtgc cgtaaatatc gcagccattg
    24781 aggcaaaaat aaatcaagcc attgacgatt atcagaagaa accaaccttc cataatagta
    24841 cggctaaaac agtattaggg caatcgacta ctgtgacgga tacgaataat ctgaatttgt
    24901 cagagttcga tgaggttgtg gaaaatgccg caaacattga ttatcgtgtg aatggtaatc
    24961 agttgataat caccccaaat gccaattcaa aagaaagtgg tgtgctgacg cttaagaaat
    25021 ctgctggtac cggaacacct gtcgcctata aaaaggctgg ccaacagact ttaatggccg
    25081 gggcgattga taaacctaat acctacacaa tcaaaattga tgtggaaacg gagggttctt
    25141 taaagatcaa aaaagtcgat aaagaatcgg gtgatgttgt accaggaacg gttttccatt
    25201 tagactttgg aaaaacttta cctgcaaaag acgtgactac tgacaaagaa ggcattgcta
    25261 cattggatga gattcctcat ggtacaaaag taaccattac cgaaaaatca gtaccggcgc
    25321 cttatacgat tgataccacc cccatgacta ccaccattaa agcaggcgaa accatttacg
    25381 tgacttcaaa aaacgcacgg gaaaaagggc aaatcattct ggataaaagc ggagttgaaa
    25441 cagggagtga tctttggaat gacaattatt ccttagctgg taatacgttt gccattcgca
    25501 aagacagtcc aaccggtgaa atcgttcaag aaatgaccac ggatgaaaac ggtcatgcgg
    25561 aaacaccaaa agagattgct aatgcgttgg aattgggaac ctattatgtg accgaaacta
    25621 aagctagtca tgggttcgtg aatacgttca aacctgtaaa agtcgaattg aagtatgcca
    25681 atcaaaccgt agcccctgtt accagtaacg taaaagggca aaatcaagaa gtcactggcg
    25741 aaaccacttt gacgaaagaa gacaaagata ctggggataa agcacaaggt aaggcagtct
    25801 ttgagggtgc tgaatacacc ttgtttactg ctaaggatgg aaaagctgtt aaatggagtg
    25861 aggcttttaa accagagtta gcgaagggaa cgaaaggttc tgatgaaacg gtcactttga
    25921 ctttagacga aaagaaccaa gttgccgtta aacatttagc tatcaacgag tattattggc
    25981 aagaaacaaa agcgcccgaa ggatatagcc ttgatgaaac aaagtatcct gtatccatca
    26041 aaaaggtgga tgatagcgaa aaaaatgccg tgattacccg aaatgttacg gcaaaagaac
    26101 aagtgattcg ctttggcttt gatttcttta aatttgctgg atcagctgcg ggcactaccg
    26161 aaactggctt caatgatctg acctttaaag tgtcgccatt ggaagggacc aatgaaatca
    26221 caggtgccga agatgaagcg accacagctt ataacgagca attagggttt gatggttatg
    26281 gcaagtttga aaatcttcct tatggggatt atttacttga agaagtagaa gccccagaag
    26341 gttttcaaaa gattacgcca ttagaaatcc gttctacttt taaggaaaac aaagaagact
    26401 ttgtgaagag tgagtatgtc tttaccatta ccgaacaaga ccaaaaacaa ccaattaaaa
    26461 cggtgaccgt tccttacgag aagctgacga acaaagcatt ttctgttagt ttgaaccgtt
    26521 taatgctcta tgatttgcct gaggaagaag atagtttaac ttctcttgcg acttggaaag
    26581 acggaaacaa agaattaact tctctggatt ctaccgagct agttgataaa ttgagttaca
    26641 atctacatga aatcaaagaa gactggtatg tggtagcgca agccattgat gtggatgcga
    26701 caaaagctgc ccaagaaaaa gatgaaaaag ccaaaccagt ggtgattgca gaaacaagcg
    26761 caacgttggc gaacaaagag aaaacaggaa cttggaaaat tcagcataaa ttaaccgccg
    26821 aacaagtttt aaataaaact attgtcttat tcaactatgt gtatgaaaat aaggaagctt
    26881 ttgaagcagg cgataagcca gtagcgaagg atgttagctt gaataaccaa gcccaaactg
    26941 tcagttgtac agtggaacac catgtttcta tccaaacaaa ggcccaccta gaaaacggtt
    27001 ctcagacctt tactcatggt gatgtggtgg atatgtttga tgatgtgtcg attactcatg
    27061 atgtactgga tggctcaaaa gaagctttcg aaacaattct gtatgcttta ttaccggatg
    27121 gtacaaacaa agaaatttgg aaatctggca agattgatta tgaagtaaat gataaagaat
    27181 tcaccaaaac tgtgcttgcg aaaaaagtag ataccggaaa atatccagaa ggaaccaagt
    27241 ttacttttgc ggaaatcaat tacgataaag atgggaatat caatggaaaa cacaatgaag
    27301 atttgaaaga aaaatctcaa accttaacac caaaagaagt tccaaccata ctaagtacgc
    27361 cgaaacaacc ggaaacacca actgttccaa gtggctctca agaatctagt cccacagtga
    27421 agaccttccc tcaaaccggg gagaaaaatt ccaatgtttt actgttcatt ggttttactt
    27481 tgatttttgc gacggcgggc tattatttct ggaatcgccg gaactaaggt gatgagatga
    27541 tacaagtaaa tcctaagaac caacgtgcgc gaccgcctcc taaaaaagta gtcgcgctat
    27601 ttttgtatcc aaaactaaca atgaaaagag gaaaagaaga tggaattgaa atttgtcgtg
    27661 ccagatatgg ctgaaacctt tggaaagtta agttatgccg gtgaaggaga aattataaca
    27721 gaaggatatg gacggaatac tacggtgatt ggtcgcagtt atcatttgta ttccagtaag
    27781 caacgggccg atgatattga ggtggtagta gctgcggaag ccggtgaaaa ggattttgat
    27841 caagaccaac cgctaaaagc cgtgaatccc catttggttg ccaaaggcta tgagattgag
    27901 aatcgtgggt ttactgatta cgtgttgtat gttgatgatt tagtgaaagc ataggaggaa
    27961 aagaagatga gattaacaga aggtattgtc gtagattcag gtttaacatt tgggaagttg
    28021 cgtttttccg cattacgccg agaagtacgg aagcaaaatg aggatggaac aattggtaat
    28081 gaggtaaaag aacgaaccta taatttaaaa tcctctgcac aagggcgaat gattcaagtt
    28141 agtatcccag ctaatgtacc cttacgcgag tttgcttatg atgcagaggt agagttggtc
    28201 aatccaatcg tggataccgt tgccaattat gtttttcgag aaggaactac cgttaattgg
    28261 ttcattaagg cggatgattt agtcttgaag cggcagccga atcaaggaaa tcctacgaac
    28321 caaaatgaaa gaaagaaata ggtgaagatt atggaaatta atgttgaaca tattttagat
    28381 tgtctcgatc aatatggaaa aggcgagctg actgaggaac aactcgttaa agcattaacc
    28441 tctgatgaaa aaatgtttct gattatacat caaggtttac ttggagaaaa tagcgacagt
    28501 gaagaagact ctgatgtact agactggtta ggagaagaag agtctttctt tatagttgtc
    28561 gaagtaaatg aaaacttatg tcggcaggca gaatccattt tggaagaaat tggtgtggag
    28621 atgccggatg ctattgaagg ctttttaaat caattggttg aaacgaaaca actcccctcc
    28681 tcgtagcagt aaatgactag cataactttt tgaaattgcc taatgaaatc ctattgggct
    28741 ttttcgattg gaggtggaaa ccatcaaaat gtacaaagga catcgcatac gcgcaggaga
    28801 tcagcacttg gtttatcact ttgttcttgg ctggctactc gctctgttca tcggttggat
    28861 gagcgtcttt tattttcaag agtttagaca atttgatatt tctaagctat cactttctac
    28921 tattgaaatc gtttggtcta taaaagattt agtatgtctg cttggaagtc tcgctttttc
    28981 aggagctatg atattgcttt atattcactt ttttcttgat cattggcgaa gcttgtggca
    29041 tcgacaaaag ctagctcgga tgattctaga gaaccattgg tatgaagtga aacaaactca
    29101 aagtgaaggc tttttcaaag atttgaatag tagccgaacc agagagacta tcagttactt
    29161 ccccaaaatc tgttatcgaa tgaaagatgg attactgtct attcgcgttc aaatttcact
    29221 tgggaaatac caagatcagc ttttaaaatt ggaaaagaag ttagaaagcg gactgtattg
    29281 tgagttagta gaaaaggaac tcaaagactc ctatgtggag tatactttac tgtatgatat
    29341 gatcgccaat cgaattggga tagacgaagt agtggcagaa aatgggactc tgcgattgat
    29401 gaaaaatcaa gtatgggctt atgattcttt accccatatg ttaatcgctg gtggcacggg
    29461 tggagggaag acctatttcc tcctcaccat tatcgaagca ctattgaagt cagatgcaga
    29521 attgtttatc ctcgatccca aaaatgcaga tttagctgat ttaggtacgg tgatgcctca
    29581 tgtttattca caaaaggaag aaatttctgc ttgtgtggaa gatttctatg aacgtatgat
    29641 ggctcgtagc aaggcaatga aagaaatgtc taactacaaa acaggagaga attatgccta
    29701 tcttggactt ccacccaact ttttaatctt tgatgaatac gtggcttata tggagatgtt
    29761 aacgacgaaa gaaagtgcgg tgattttgaa taagctcaaa caaatcgtga tgttgggtcg
    29821 tcaatctggt ttctttctga ttttggcttg ccaaaggccg gatgcgaaat atttaggaga
    29881 cggaatccgc gatcaattca actttcgcgt ggctttgggt cgtatgagtg aactcggata
    29941 ttccatgatg tttggggagg tcgaaaaaaa cttctttatg aagcacatca aaggtcgagg
    30001 ttatgtggat acaggtggca gtgtgattag tgagttttat acgccacttg taccaaaagg
    30061 gtatgatttc ttagattcaa ttagtaaatt agaaaagatg aatgattgag tgatcttcaa
    30121 tactgacttt atacttcaag taacgtataa tacttttata tagtaggaag aaaggggata
    30181 attaatatgg aaagtaatga ttcttattta gagtatgcaa aagcaaataa ggataagttt
    30241 atttcttata ttatcaaagg gaaatccccc gaagttggac gagacgcagt ttttatggca
    30301 gggagtcctg gggctggaaa atcagaggtt gccattggat tagctgagaa ttatgataac
    30361 catgtgatta ttgatgcgga ttactttcgc acccagtttc ctgattataa tggaaaaaac
    30421 tctagtctct ttcaaaaagc atcttcttgg ttggtagagc aggcatttag atatgtctta
    30481 gatcatggct attcttttat tctggatgca acttttgcaa tattaagtgc tgaaaaaaat
    30541 atctcgcggg cgttgaaaaa acattttcga gtgacgattt tctatgttta tcaagaccct
    30601 aaaatcgctt ggaaatttac acaagaacgt gaaattgcag agggacgtca tgtgccgaaa
    30661 aaggcattta ttaacgcttt tttcaaatca agagagaaca ttgcaaaagt aaagagtaaa
    30721 catccagatg tattgcttca tatcataata aaagattata cgaataatat ttccgaagtt
    30781 cattatgcta cggataatct agaattattt ttaccactag ggtatagtag gaaagatttg
    30841 gaggttcaat tacatgactg agaaagaaca agtaacgaaa attgtaaaga aatacaataa
    30901 gagcattgcg gatttatcgg aaaatgctac tgcaaaagag ttcaaaacgg tgattaaata
    30961 tgtagccgat caggccaatg aaaaacaacg gaaattagtt ggattaaata aaaaatagta
    31021 ggacaaatta ataatgtatg agccaagagc aaaatgttct tggctttttt ctttgcaatg
    31081 aatgaggtaa tgtatgagat ttttagattt attcgccggt attggtggct ttcgtttagg
    31141 tatggaacag gcgagccatc attgtatcgg tttttgtgaa attgataaat ttgctagacg
    31201 tagttataaa gcaattcatg atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt
    31261 atcagatgag tttattcaat ccctcggacc agtggacatc ctttgcggcg gatttccgtg
    31321 ccaagctttt tcaattgcgg gaaagcggca aagattttcc gatactcgag gtactttatt
    31381 ctttgaaatc gctcggttca ccactcttct ccgacctaag cttttattcc ttgagaacgt
    31441 ccgaggattg ctcaatcacg aaggaggggc tacgttcgag acgatcctcc gaacgctgga
    31501 tggattgggg tatgacgtgg agtggtaggt gcttaactca aaggcctaca ttccccaaag
    31561 ccgggagcgg atattcctta tcggacattc tcgagacgca tgtaccgaac aagtatttcc
    31621 tatcattgga ccgtcttcaa catctgatca aaacatcaga aatttggtaa atatcaatcc
    31681 ttccaatcga ggaatgggtg gacaagtcta tggttcagat ggcgtagcgc caaccttaac
    31741 aggtgatgaa ggaatcaaaa tcgcactgcc agcaagggat ggaatctctg tagcaggact
    31801 attaccggga aagtttgaac aaggtaatcg agtttatgaa gttactggaa ccgcccccac
    31861 cttgtcaacg aaacaaggtg gtacaaaaat catgattcga aaaaatgaaa cagaatatca
    31921 agaggtgaag ccagaagaca gtgtgaatct ggcttttcct aattccacaa gtcgccgagg
    31981 tcgtctggga aaacagagtg ttcatacctt actaacagga gaccaacaag cgatcgtcac
    32041 ggatcagtat caaattcgga agctaacacc tagagagtgt tggcgacttc agggctttcc
    32101 cgattgggct tttgatcgag attctcaagt caattcagat agtcagttgt ataagcaagc
    32161 cggaaactct gtaacagttc cggtcatttt tgatattgcc agaagattaa aagagttgga
    32221 tgtacatggt ttatgatgag ttgattggag aaatttattg ggtgattgga aagattcagt
    32281 cagacccaga attagaagaa caattgcgac ggttaaaatt tgatattcgc aagaatggtg
    32341 tcaaagtcgc tggcgatcca tatttaatga atgaggagac ggatgctcga attgaaataa
    32401 atcaagtaat tgcagaattt gagcggattg cggaccaagc aaaagaacca gatattcgtc
    32461 aatacttgtt agaaatgaaa gcagagttgg aagtaaatgg tattacagat gagtaaccta
    32521 aaccaacaca actgactgtt aggcgctgac cgcggaggat tcgcgaagca gaaatcctcc
    32581 gtggtgctgt gcctgttcat ggacggcgtt agccgaccat gatgtctcac ccccgtatct
    32641 aacagggggg tacaaaaaca caaaaaataa gtaagcaacc attaagaatg cttgttgtat
    32701 caaagatagt aagtaatttg tataggtgtt aactcatttg aaaaagttaa caccttgaat
    32761 ttggaggtga gaacttggca caacggaatt tagattatcg gctattaaaa gaccgacgca
    32821 acgaatacgg cgtttcacaa aataaattgg ctacggcttg tggacttagt cggccgtact
    32881 taaatcagat agaaaatggt ggcgtaaccg catctataaa aaccatgagg aaaatttttg
    32941 accaactgga aagctttaat cccgatttgc ctttgacgtt actgtttgac tatgtaagga
    33001 ttcgctttcc cacaacggat gcacgaaaaa ttattcaaga aattctccat ttgaaatttg
    33061 agtatatgct ccatgaagat tatgcctttt actcctatca agaacagtat gtcatgggtg
    33121 atattgtggt gatgttgtct catgaagaag ataaaggtgt gcttttagaa ttaaaggggc
    33181 gaggttgccg acaatttgaa acttttttac tcgctcaaaa gcgtagctgg tacgactttt
    33241 tcgaggattg tctaaaggcc ggtggcgtga tgaaacgctt ggacttggca atcaatgatc
    33301 gagtggggct attggatata cctgagctaa cgaaaaaatg tcagaaggaa gaatgtatct
    33361 ccttgtttcg tacctttaaa agttatcgtt ccggggaact tttaaaggct gatgaaaagg
    33421 acggcatggg caatacctta tatatcggca gtctcaagag tgaggtttat ttctgtttgt
    33481 atgaaaaaga ctatgaacaa tatatcaagt tagggattcc attagaccaa actgagacga
    33541 aaaatcgttt tgagattcga ctgaaaaatg accgtgcttt tcatgcgatt caagatttgt
    33601 taaaaggtcg tagcattgag agcaccacgt tttccattat caatcgttac ttgcgatttg
    33661 cggataaagt agaaggtaaa aggcgaacca actggccctt gaatgaacaa tgggggcgtt
    33721 ttattggacg gaatcggaaa gagattcaat taacttccga gccaaaaccg tataccattg
    33781 aacgaacctt gaattggcta ggccgacaag tcgcccctac gtggaaaatg gctaaggagt
    33841 tggatcgatt gaatcggaca acctatatcc aggatatggt acagaatgca cgcttatcag
    33901 accgacataa aaagattttg gagcaacaaa gtatggcaat tgaaaatttg attatatgaa
    33961 aagaggaatc gtcatgggaa ttgtaaaaga tatttttttg gtatttggtg gcagtatgct
    34021 tggtgttact gtgatgtgct tgatgaatgc aagtgcagca gcggatcgag caatggaaaa
    34081 gaaaggaaag tgacaagatg aatttcggac aaaattt
//
LOCUS       DACDRK010000023        34029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDRK010000023 DACDRK010000000
VERSION     DACDRK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.023"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWJ77_001203"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWJ77_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9452834.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWJ77_001204"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWJ77_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9452835.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWJ77_001205"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWJ77_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9452836.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWJ77_001206"
     CDS             1642..2274
                     /locus_tag="IWJ77_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9452837.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWJ77_001207"
     CDS             complement(2276..3181)
                     /locus_tag="IWJ77_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9452838.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWJ77_001208"
     CDS             complement(3266..3919)
                     /locus_tag="IWJ77_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9452839.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWJ77_001209"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWJ77_001209"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9452840.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWJ77_001210"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWJ77_001210"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9452841.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWJ77_001211"
     CDS             6168..7253
                     /locus_tag="IWJ77_001211"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9452842.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWJ77_001212"
     CDS             7250..8314
                     /locus_tag="IWJ77_001212"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9452843.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IWJ77_001213"
     CDS             complement(8563..9366)
                     /locus_tag="IWJ77_001213"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9452844.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWJ77_001214"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWJ77_001214"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9452845.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IWJ77_001215"
     CDS             complement(10244..11251)
                     /locus_tag="IWJ77_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9452846.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IWJ77_001216"
     CDS             complement(11258..11902)
                     /locus_tag="IWJ77_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9452847.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IWJ77_001217"
     CDS             complement(12002..12760)
                     /locus_tag="IWJ77_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9452848.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWJ77_001218"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWJ77_001218"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9452849.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWJ77_001219"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWJ77_001219"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9452850.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWJ77_001220"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWJ77_001220"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9452851.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWJ77_001221"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWJ77_001221"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9452852.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWJ77_001222"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWJ77_001222"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9452853.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IWJ77_001223"
     CDS             18158..19027
                     /locus_tag="IWJ77_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9452854.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IWJ77_001224"
     CDS             complement(19153..20052)
                     /locus_tag="IWJ77_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9452855.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IWJ77_001225"
     CDS             complement(20217..21041)
                     /locus_tag="IWJ77_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9452856.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IWJ77_001226"
     CDS             complement(21052..22347)
                     /locus_tag="IWJ77_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9452857.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IWJ77_001227"
     CDS             complement(22363..22842)
                     /locus_tag="IWJ77_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9452858.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IWJ77_001228"
     CDS             complement(22848..23876)
                     /locus_tag="IWJ77_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9452859.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IWJ77_001229"
     CDS             complement(23893..24999)
                     /locus_tag="IWJ77_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9452860.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWJ77_001230"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWJ77_001230"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9452861.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IWJ77_001231"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="IWJ77_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9452862.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IWJ77_001232"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="IWJ77_001232"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9452863.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="IWJ77_001233"
     CDS             29970..30668
                     /locus_tag="IWJ77_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9452864.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="IWJ77_001234"
     CDS             complement(30836..33475)
                     /locus_tag="IWJ77_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9452865.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         9598 a         7456 c         6153 g        10822 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggctt
//
LOCUS       DACDRK010000024        33220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDRK010000024 DACDRK010000000
VERSION     DACDRK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.024"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..351
                     /locus_tag="IWJ77_001235"
     CDS             <1..351
                     /locus_tag="IWJ77_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9452866.1"
                     /translation="VQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRINS
                     IKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIVDK
                     AVFEAFQRGLNKCD"
     gene            complement(439..831)
                     /gene="rpsI"
                     /locus_tag="IWJ77_001236"
     CDS             complement(439..831)
                     /gene="rpsI"
                     /locus_tag="IWJ77_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9452867.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(847..1290)
                     /gene="rplM"
                     /locus_tag="IWJ77_001237"
     CDS             complement(847..1290)
                     /gene="rplM"
                     /locus_tag="IWJ77_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9452868.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(1809..2276)
                     /locus_tag="IWJ77_001238"
     CDS             complement(1809..2276)
                     /locus_tag="IWJ77_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9452869.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(2396..3934)
                     /locus_tag="IWJ77_001239"
     CDS             complement(2396..3934)
                     /locus_tag="IWJ77_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9452870.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4014..4700
                     /locus_tag="IWJ77_001240"
     CDS             4014..4700
                     /locus_tag="IWJ77_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9452871.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(4901..5428)
                     /gene="msrA"
                     /locus_tag="IWJ77_001241"
     CDS             complement(4901..5428)
                     /gene="msrA"
                     /locus_tag="IWJ77_001241"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9452872.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(5512..6189)
                     /gene="pgmB"
                     /locus_tag="IWJ77_001242"
     CDS             complement(5512..6189)
                     /gene="pgmB"
                     /locus_tag="IWJ77_001242"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9452873.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6186..7316)
                     /locus_tag="IWJ77_001243"
     CDS             complement(6186..7316)
                     /locus_tag="IWJ77_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9452874.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(7316..9499)
                     /locus_tag="IWJ77_001244"
     CDS             complement(7316..9499)
                     /locus_tag="IWJ77_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9452875.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(9496..10521)
                     /locus_tag="IWJ77_001245"
     CDS             complement(9496..10521)
                     /locus_tag="IWJ77_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9452876.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(10566..11393)
                     /locus_tag="IWJ77_001246"
     CDS             complement(10566..11393)
                     /locus_tag="IWJ77_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9452877.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(11390..12457)
                     /locus_tag="IWJ77_001247"
     CDS             complement(11390..12457)
                     /locus_tag="IWJ77_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9452878.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(12483..13319)
                     /locus_tag="IWJ77_001248"
     CDS             complement(12483..13319)
                     /locus_tag="IWJ77_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9452879.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(13316..14185)
                     /locus_tag="IWJ77_001249"
     CDS             complement(13316..14185)
                     /locus_tag="IWJ77_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9452880.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(14255..15538)
                     /locus_tag="IWJ77_001250"
     CDS             complement(14255..15538)
                     /locus_tag="IWJ77_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9452881.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(15552..17237)
                     /locus_tag="IWJ77_001251"
     CDS             complement(15552..17237)
                     /locus_tag="IWJ77_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9452882.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            17512..18537
                     /locus_tag="IWJ77_001252"
     CDS             17512..18537
                     /locus_tag="IWJ77_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9452883.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19132..22785)
                     /gene="rpoC"
                     /locus_tag="IWJ77_001253"
     CDS             complement(19132..22785)
                     /gene="rpoC"
                     /locus_tag="IWJ77_001253"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9452884.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(22864..26481)
                     /gene="rpoB"
                     /locus_tag="IWJ77_001254"
     CDS             complement(22864..26481)
                     /gene="rpoB"
                     /locus_tag="IWJ77_001254"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9452885.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27209..28177
                     /locus_tag="IWJ77_001255"
     CDS             27209..28177
                     /locus_tag="IWJ77_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9452886.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28195..29100
                     /locus_tag="IWJ77_001256"
     CDS             28195..29100
                     /locus_tag="IWJ77_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9452887.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29097..29903
                     /locus_tag="IWJ77_001257"
     CDS             29097..29903
                     /locus_tag="IWJ77_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9452888.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30060..31028
                     /locus_tag="IWJ77_001258"
     CDS             30060..31028
                     /locus_tag="IWJ77_001258"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9452889.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(31345..31869)
                     /locus_tag="IWJ77_001259"
     CDS             complement(31345..31869)
                     /locus_tag="IWJ77_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9452890.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32156..>33220
                     /gene="scm"
                     /locus_tag="IWJ77_001260"
     CDS             32156..>33220
                     /gene="scm"
                     /locus_tag="IWJ77_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9452891.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         9347 a         7132 c         6251 g        10490 t
ORIGIN      
        1 gtacaagctg atcaattgtt gaaattaaaa attaagcaaa cgttagatca gtatctcttt
       61 acttatacta aaccatctgg agaagtaaat tgtcctgttc atgccgatta cctaaactat
      121 cgaatcaatt caattaaaag aaggcatcct gacttagttc atttatctcc acacaaactc
      181 cgtcatactt atgcaactat agctagacaa ggtggagcag atatgaatca aatttcaaat
      241 gctttaacac actcagatat ttctacaaca aagatatatg tgaacacacc agatatagtt
      301 gataaggctg tttttgaagc ttttcagaga ggattgaata agtgtgattg atatgtgttc
      361 ttaaatgtgt tcttaaaaac aaaagaagca ctttccaaat aattgaaaag tgccagaaac
      421 gttgatatat aaaggatatt aacgttttga gaattgtgaa gctttacgtg ctttcttaag
      481 acctggtttt ttacgttcga ccatacgtgc gtcacgagta agtaagccag cacgtttaag
      541 agctaaacgg aagtcaggat caacttctag caatgcacga gcgataccat gacggatagc
      601 tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat atgcgccttt
      661 tgtttcagta acagcgaaag gttgattgat tacttcacgc aagtcagcat gtggaatgta
      721 ttcttcaaca tcttttttat ttacagtaat tttgccagtt cctggtacta gacgtacgcg
      781 agcaactgcg tttttacgac ggcctgtgcc gatatattga acttgtgcca atgaaattcc
      841 ctcctattag attaagtttg tgatgtctaa aacttctggt tgttgtgctg catgtggatg
      901 ttctgctcca ccgtatacgt tcaattttgt aaattgtttg cggcctaaag tgtttttagg
      961 aagcatacct tttacagaag tttcgatcaa acgacgagaa tttttagcac gcaattcgcc
     1021 agcagtgatt gatttcaatc ctcctgggta gttgctgtga cggtaataaa ttttgtcagt
     1081 cgctttttta cctgttaatt tcactttgtc tgcattgatg acgatgacga aatcgccagt
     1141 atccaaatga ggtgtgaaag ttggtttatt tttaccacgt agtacagatg caacaactgt
     1201 tgaaagacgt cccaatggaa catcagttgc gtctactaca taccatttac gttctacttc
     1261 gcctggtttg gccatatatg ttgtacgcac gttattttcc tccaatttaa gttctgatgt
     1321 ttgacataca cttcgagttt ccggggctct ttgtggggca aacaatacca tttaacatat
     1381 tacccgctaa tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac
     1441 tttacttctt ttctttcttt tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa
     1501 agaggctgtg acaaaagtct tgtcacaacc ccttattttg aataaacggt gttctcctgc
     1561 caactcctcg gtcacaagtc acaatatcca taaaacataa gaagctgttt tttaaaattg
     1621 tttcttgtga ctcggagctg aacggcaggc ttacaacctc ttggtaaacg gtgttcaatc
     1681 agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgacgtttg
     1741 tttctctaat actcaggtca aaacgctgat ttcacaacct ctttttcggt caagttcaac
     1801 aacagttttt acgcgtcgat ctctggcgct ttgcccaaat gagcttggat catccagata
     1861 cgtttttctg tggctgtttt gaattcgata aagatatctt gtgttggatc gtcgccttct
     1921 tctccagaaa cttcgattcc tttttggtaa agatctgcta gataacgata gcctgcaaca
     1981 agtgtttcca aacgttctgg aatagtacga tcccagcggc caatttcatc agggattttt
     2041 gtgttatctg caaattcacg caatgtagaa taaggtgaac cgtctaatgt gatcaagcgt
     2101 tcggatactt catctaactg agcgttcaaa tcatccatga actcatccat cattgggtgc
     2161 aatgtaagga aacctggtcc tctcatgtac caatgtgttt ggtgaacaac aacagacatt
     2221 tggcttagat ctgcaactaa ttgatttaat acttctttgg tttgttggaa tttcatcttt
     2281 cttgcctcct aacattttct tacacttaaa ataatagtag actttcaatg aaaagtacaa
     2341 caaaatcatt tacctgagaa cgattttcag ttcgcttttt tatccgtaaa gccaatcatt
     2401 tttcagaatc agctaattct tctcgcagtt gagcaaattg ctgataagtc tgtgctaaat
     2461 gcttttgtgt cgaaacaaat aatggataga ttttctgata ttttttatga tcagtttctt
     2521 gtactccaac ttgctctttc aattctatca attcctgtgc ttcactgata ctttggatct
     2581 ttccgatgct tctccagcct aaaatcacag ctcctaatgc ggatgcttct gtgacagtag
     2641 ggaaaacaag cgggtaaccc agtacatcag ccatcatctg acgaaaaatc ggagattgag
     2701 caaacccgcc tgtagcacgg atctctgtga gctgattttc ctggcaaact ccttttaaaa
     2761 tccgataaag gttgagacag atcccttcta gaatcgcctt catcatttct ttttctgtat
     2821 gttgccgctg taaaccgata aaactgccaa acgcatctgc gtcccaaagc ggtgctcgct
     2881 caccaagtaa atagggatga aaaatcagac cattggcacc cgctggcaca aaagcgatag
     2941 ccgaaagcag tgcatcatat ggattttctc ctttttcttt agcagattga acgacttcct
     3001 gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc attgctagtc gctcctccca
     3061 ctacccaatg tttctgatcc agaacataac aaaacgtctc catttctgga tggaactgag
     3121 gctgagtagt aataaaacga agtgctccgc tcgttcctac ggtcaaagcg gcgatcgtat
     3181 caccaaaagc acctaaccct agattagaaa gcggcccgtc tgcccctccc aaaacaaatg
     3241 atatatcatt tggcaaacct agttctgctg ctgtttttga tggtagacct gttacttgat
     3301 aggtggaagg aaagacttgc ggcaattgct ctattcgaat gttcaagtag tcaagcactt
     3361 cttctgccca ttggaattca tggatatcaa aatatccagt tcctgaagct gttccgtagt
     3421 cgctgatcag ttcgccaaac aatctataaa agatatagtc tttgataccg acaaaataag
     3481 cagttttttt gaataattca ggttggctgt tttgcagcca cttgattttg ttcaatggtg
     3541 tcattggatg aatcggcgtc ccagtctgcc gataaaattc tttgccaagc acggtatatt
     3601 tcagtttttc tgcttcgtcc gctgctcggt tatctgccca agtgatcatt cgagataacg
     3661 gtgttttctc ttcatccaac aatatcaggc catgcatcgc agaggagaag gagacaaatt
     3721 gaatgattgt gtctgaaggc aactgtttaa ttacttcttt tataacggtg cagacacctt
     3781 gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt ttcttggatc aaagggtagc
     3841 gaaaataagc ggatgctgct tcttgggctt catctgtgaa agcaactgct ttggtttgtg
     3901 tggttccgat atctaatccg attgaaacgt tcattttttc acctcaaatt ttatgtatgt
     3961 acaaaaattt agtttaagct tgcgtatctt ggttttgaaa agggggagat ggcgtgttac
     4021 tttattttat cattggttgc tgtatcggat catttttatg tttaaccgcc cagcggattc
     4081 cgttaggtca ctctattatt tatcctagat ctcattgtgt caaatgctgt cgttcgcttt
     4141 cttggtatga attgattccg attctctcga tcatcgtcca acgatttcgc tgccgctatt
     4201 gcaggtgtcg gttgcccttc tactatttgt tggcagaagc cttatgtggt ggtctttttg
     4261 cctggttctt tactttttct tccgcaagaa atttttcgac gttcatatgg atgctttcgg
     4321 ctttgctatt ttcgttgatg gatttatttt acttcatggt ccattctcac acgctattct
     4381 gttcttgggc aattttatgg attttttggt tgcaggccgg cgtcttccaa tggcaaagtg
     4441 tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc tatctcggag
     4501 ctggcgatac tctgttgatg ctttcttgga gtggtggctt gtctctagaa gagttgcttc
     4561 agatcttgtt tcttgctagt ctgtttggaa tatccgtttt ttctgtttac tttatctgtc
     4621 ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt gagcagtgcg ttgctgattg
     4681 tccttcatct taggtattga aaaaaggttg tgacaaacga cttgtcacaa cctctgatat
     4741 atagtgttct cttaccaaat ccttgtgact tggagctagg cgacaggttc acaacctctc
     4801 cactaaatgg tgttcaatca gctgaccctc agtattagct caaaatgctg gttttacaac
     4861 ctctgatata cggtgttcca taagtagctt tttcggcttc ttatttcttg acgccccaat
     4921 gtcgacgaag gaaacctgca cgtccagaac cttctcgata aagattgtaa tggagcttgt
     4981 ttttcttgta ataatcctga tggtaatctt ctgcaggata aaattcgctt cttggttcga
     5041 tttttgtcac gattggctta tcgaatcgcc cgctgtcttg caatgcttgt tttgaacgct
     5101 ctgcaatatt tcgttcttct tcatcattat agtagatcac tggacgataa gaatcaccgc
     5161 gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag atttccacca
     5221 actgatcata agaaataact tctggatcat attcgatctc tactgcttct gtatgccctg
     5281 ttgtgcctgt tgttacttgt tcataagttg gatttggtac atgcccgcca gtataaccgg
     5341 atgtcacaga aatgatccct ggctgctctt caaatggttt gaccatacac caaaagcatc
     5401 cgcctgcgaa aactgctttt tttgtcatat aaaatccccc ttttttctca tcataccttg
     5461 atttccaaaa ggaggataac gttttgcttg tgacagaaaa aacggaaact attatatacg
     5521 tgatacggct tctgataaat gccaaaacga acgtacttga tcaaaaacgg ttaaatcagg
     5581 aatcacatat tcagcagaag gaagatttat gttttcacct attcccaata catgcatgtt
     5641 accagccaaa gctgcttgac aaccagcttc tgaatcttca atgaccaagc atgcatttgg
     5701 cggtacattc aattgctgtg ctcctttgag aaaaacttct ggatcaggtt ttgcttttga
     5761 tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt tttctaaaat
     5821 gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg atgtttgttt ttttcaagta
     5881 ctcaagaaca gctattgcac caggcaaaac ggatgtttca tccatttttt gaatcgcttg
     5941 aagatataga ttgttttttt gctcagcaaa ggctgatttc tctttttctg agaatttctg
     6001 ttccattttt ccaaatgaca gcaatcgctc tagtgactcg gttctggata ttcctttcag
     6061 ttgctcgttt tctttctcag tgaatggaat ggacagctgg ttagctaaaa ccagccacgc
     6121 ttcgtaatga taacgagctg tgtctacaag aacgccatct aaatcgaata gtgctgcttt
     6181 gattttcatc gattacctcc ttgaaaatga acgacttgaa gtaaagatgc gatgttttta
     6241 gctgtgcgat gaagattatc agtggcttgc tgtaaagctt cttgtaaagg cacagctgca
     6301 ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg ctttttcgct tcctttacca
     6361 acacttccgc aaatagctat cacaggaacg ccctctggta ctcttctagc aatcccaact
     6421 ggcgcttttc cttttagact ttgtccatct agacgtcctt cgcctacgat tacgaggtct
     6481 gcttgctgta cccgtttatc gaactccagt aaatccaata cgtagtcgat gccagagact
     6541 attttccctg aagcaaatgc taacagtcct gcacccattc caccaccagc actactgccc
     6601 gatgcctcga ttacttgagg ggcaaatctc atataaaaat catgcatggc ttggtcagcc
     6661 actgctaatt cattttcttt caagcctttt tgtggtccga aaacaaaaga agcgccttct
     6721 tttccgcata aaagattagt aacatcagta atgacagtga ttttggcttt aatcagctca
     6781 ataggcacgt tcgttttatc aactgtagca attttaccta gattttctcc cagtgcttct
     6841 actttttgat tatcttgatc gtaaaaagca taacctagac cttctgccat gccgatgccg
     6901 ccatcgttgg tactacttcc gccaacgccg atgatgatgt gtcgaacacc ttgttgcaac
     6961 aattgtcgta tcagttctcc tactcctcgc gtggataacc taagtggctg ttgttggttt
     7021 agtgggacat ctttcaatcc gacgatttct gccatttcaa aaatcgcagt ttgaccgtct
     7081 gaagcgtact ggatttcttt gtttttgcca aatgcattag taacgactgc tgtttgccgc
     7141 ttcaacctta aactgtctgc taatgatgaa accgttcctt ctcctccatc gcctataggt
     7201 agcagatcat attcagcctg aacaaaaact tcttgaaaac ctgattgaat ggcttgtgct
     7261 gcctctaagg ctgtcagact ttctttgaaa gaatctggcg caatcagaat acgcattatt
     7321 ttcccctccg ttccaagatc agttcttttt ccagctggta ttggtctcca ttgacaaaaa
     7381 taggtaatgt ttctttcgtc tcttttttta ttactatttg tttttttgtc aaaacaaata
     7441 gcaacctgtg atttttccaa ataaaaggaa aagaaatttt ttcccatgct gaaggaagtc
     7501 ttggagaaat agaaagagtt ccattcgtta ctgtaatacc tgaaaaacca aatataactg
     7561 ctagccatag tgctcctaat gaagcagcat gaattccgtc atctgatgaa tgcggatctg
     7621 gtcctaaatc aatcaggcac gcttcttgga ataaatgata cgcccattcc atttcttgga
     7681 tacggcaagc ctctatggcg tgaatcgctt tactcaacga tgagtcgtgg attgttttgt
     7741 tttcatagta atgcaaattc ttagaaacga ttggttgaga aaaatactca ggtaataagt
     7801 ataacagcat gacaagatct gcttgcttgc tgacttggag ttcatttact tcgctacggg
     7861 cataatcaag taaaatagac tggctgccag gacgttcttt gtaagtggtc aaatcaataa
     7921 ctggcttact gagatacgtg tcatcttgcg gaatcagctg ttctttgtta ggcactggca
     7981 aatataactt atctaaaaaa ttttgacatt ctttcaaaaa aagcggatcg ccagcttgat
     8041 atttaacagc taaggccaca ttataatgcg ccagatagtt cgtatatgca ttattgtcga
     8101 tatattctgt gtattcatct ggaccaatga ctctgtgtat ctctaaatga tcatcgactt
     8161 ttacagctct gctcagccaa aaagctgctg tttcatgaat gagcgttttt ccctttgttt
     8221 ctaaaaacga ttgatcctct gttgcttgga catactcttg aacggcataa gcaaggtctg
     8281 cactgatatg atgttctgcg gcagctgaag caacttcttg tcttgttccg gttttgatat
     8341 tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg tgcgctttcc catgggaaaa
     8401 gtgcaccttg atagtgataa tctcttgctt tttgccgtgc gccttctaat cgatcataac
     8461 ggtacagcag caaattcttt gctacttcag gaaactgata taaaaagaaa ggcaggataa
     8521 aaatttccgt atcccagaaa acatgccctt tataaccttc gccagttagt cccttcgccc
     8581 ctacactaac acttggatca ttgcggggag tcataatctg taaatggtaa gcagcgaagt
     8641 ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt tatccgatgc tgatgccaaa
     8701 aatctgtcca ataagacatc gactgctcta aaatttctgt ataactaacg cttttctggg
     8761 cttgggcaat acttttctta aacgggtctg tttgctgaag agaggtagag agcacgatga
     8821 tcttctccag cgtaaaggac gtattttttc tgatcttttc tgaaaaggaa gcggtgatcc
     8881 ggcgattttt tgcttggaat acacctgctt tattgaattt agctgcaatc ccaatatcaa
     8941 ttttactttc tgttgtctgg taggatgcac tgatcaaatc gtcaccaaat actcgcagtt
     9001 ctttttcaac caactgctgt gtaccaaaat tggtttgctg tccatcgata cccgttttca
     9061 catctacttg gcagtcttcg gtcaacggct tgatgaccac tttcagggct gcctgatgga
     9121 gattattttg atcagcaaca cgacaagtaa tgacttccac tttttttcct tgagcagttt
     9181 caatgagcgc atagcgttta agctctccca tttctaaatc tagtgcccgc tcgtatgcaa
     9241 gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat ttttatttcc catctgatga
     9301 catctgggag attcactaac tcggctgatt ctgctcctaa aggtcggtta tatacaccag
     9361 ctgcaaacat tccccttact tgattccgat agtcttcctc ctggcttcct ctgataccaa
     9421 aaaatccatt tccaatggtc atctgactcg catacttatt gatatactct aaatcaaact
     9481 gtttttcttc tactttcata aggtaataac ttctcctgtt tctgccgaac gatataaatt
     9541 ttccaccaga ttctggatga catatccttg ccagccatct gcaatcatct gagtctggtc
     9601 accgagcgcc cgattcacaa aatgagccat acttagttca tgtgggtcat taactggcgt
     9661 ggtcttttgt aaggaaacca gctttccatc tacatcttcg aatagttcgc ctggatacag
     9721 attagcacct gcctgatcgc cacaaaaagc cacattcagt ttgtcttctc tgatattcaa
     9781 agcaaacgaa gtattcagtt ggatgatctt gccgttttct aattccagtg tgccaaaaat
     9841 tgcatcttct actgtgaatt tgcttggatc ccatttcccg aatttccctg aatttttttt
     9901 cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt actttaggaa atccagtaat
     9961 atacatcgct gtatcaagca tgtgaatgcc ataatcgatc aaaggcccgc ctccttgaag
    10021 ttctttgtcg ataaacgttc cccagcctgg aacaccagac cgacgaagcg cattcgcttc
    10081 tacgtaataa atatccccga gaacatcaag atgattcttg atcattgttg cttcgtcaga
    10141 aaaacgatgc tggaatccat aagccaaaat ctgcccgttt ttatcggctg tctctgccat
    10201 ctttcttgct tgatcagctg tcatagctgg tggtttctcg cacatcactg aagcattagc
    10261 ttcgagggca gccaatacat gttcgaagtg aaagcgattg ggtgtacaga cactcacgat
    10321 atcgggagaa atctcattca gcatttgtgt aactgatgta tacgcatgag aaaatcctgt
    10381 acgtcggcaa aagtcttctg ctcgctggct gactggatca accacagcgg ccaattctaa
    10441 gacatctgat cttttttgat aataagcagc atgtactttt tctgctactt gtccagcgcc
    10501 tatgattgct actttcttca tttatttctc tctcctttgc ttatttactt attgttattc
    10561 tttctctatt ctttctctaa atcatttaaa tgacgcgctg tttccagaca gtttttcaag
    10621 tattgcaatg attcctgata aattttcact tcattttctc ctcgcaaacg acattcatat
    10681 accatataac cttcatactc attttgaagt agcgtttgaa aatggcgagt aaaatcaatt
    10741 gcgccagatc ctggttggta gcgatgatta tctgctaaat ggatatgacc cacatactct
    10801 ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa tattcatatg ataaaaatca
    10861 gcgatgatct gcacatgttt caattgatta gagtcaatgt aatgacgcgc atccgctaac
    10921 aaattgatca tatggtcttg gtatcggttc aaaggctcca aataaataac cgtacctgtt
    10981 tcttcggcta ctttatctaa ataacgaagt gattcgctga ctgctttata gtctccttct
    11041 ttgcttcggg gtgaaaccat cggcggtaat cgataagtga acattcccca agctgctggg
    11101 ataacgattc ctttgcctcc aacctcgctt agtgcacgca aaatctcagc aatttgacgt
    11161 acaccattaa gacgccgttc ttcgataaag tcaccgatcc acccatcgta gccgttacaa
    11221 gctgtcacta ctgggatccc tgtttctttg attgcctgtt tgactgtgtc gatattttct
    11281 attaacaact taccgtcgat ttcgtattct tcaaatccac agtctttgat aaatctaaat
    11341 ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg taccgaattt cattgttctc
    11401 ctcctcggac ttgaacgccc attttgatac tttcttctgg gtgctggtct acataacgca
    11461 tgtaagcttc tgcgctttct gcaaatggca caactggatc aatgatttct tcacaatcca
    11521 agtaaccgtt catcaataat tcccaacatg tgtcctcgat tcgtttacgg ttccatcttg
    11581 ggtaatctgg gtttggttca ctagatgctc gagcaaagac gatttttgca ttattgaaat
    11641 gtgcttcgcg accaagatta aacccagctg gaaaaggttt agcaaaggca acataagaaa
    11701 tgatcccgcc atagccgatc cctattaaag aagcttgtaa tgcggcagca ctgccactcg
    11761 tttcaataac tgaatctact ccacgtttat ctgtcaattc ctttagacga aaaccgacat
    11821 ctgctttcat aggatctagc gtgtaatcgg caccgtgttt ttctgctaat tctctacgct
    11881 gcgctatggg atcgatccca acgatggtag tagcacctgc ttttttagct aattgaatcg
    11941 caatttgccc gatagcgcct aaaccgaaga ttgctacagc atcacctgct cgaacatgtg
    12001 cttcccggat accacctaaa gcgaattgag caggatcgta acatactgca tttttccatt
    12061 gatccaaccg atcaagttta cgtagtttat aattatcaac accgtttgta atcagcgttt
    12121 cacgaatggg tccataagtg cagactaact ctcctttttg atactctgtc accaaactgc
    12181 ccgtttcttc gatcgtaccg acaatcatat ttcctaattg aaattcgcca aataccacac
    12241 cgcgttggct tccttcttct cttggcaaga ataggcgcca ttcttctgaa aattcttcat
    12301 cgataaatgg acttaatcct ctaaagtcga caatttctgt tcctggtttt ggagtagcga
    12361 aaattactcg gatacgtact tcgttctttt cgatctcacg gtcttcgtaa tctttgaatc
    12421 cggctattct tggttctaat gcaactaatt ttttcatctt ttctttcctc tttcttttct
    12481 ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa taaatcgttc agataacgca
    12541 tacataatga tgactggtaa agcggtcacc aatgaagcgg ccatcattcg tccccaaatg
    12601 tagtctggtg tactgaataa tgtgttcaaa ccgattggta aagtaaattt ttcggaggtc
    12661 gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa ccataaagca ataaacgaag
    12721 acagaaatga ttccagaaat agccaatggt ataatgatcc gaataatgat ctggatccgg
    12781 ctaagaccat cgatcattgc tgcttcttca atatcagtcg gaatcgtgtc aaaataactt
    12841 tttagcatga atatcgcagt aggaagtgtt tgaacgacca tcgtgataat cagtgctgta
    12901 ttggtatcat acaaacctag accggaaatg attttaaata atggaacaac gatcaaaata
    12961 ccggaaaaca tataaacggt gtaaaagcta gcattgattt tcgagcgagc tttgaagcgt
    13021 agttttgaca aagcgtatgc cccgctgatc ccaatcacta ctgcagttac ggaggctatc
    13081 aaggagatca ctaaactatt tttgaaatac atgatgaatg gaaaaatctc agcattgaaa
    13141 atatcctgat aatgttcaaa agtccattct ttcggaaaaa tagtttgtgt cgttgcaatt
    13201 gcttcttgtg aatccttgaa tgatgtcatc agcatgatca aaaatggaaa caaattgaca
    13261 gctaatagca acaaaatcgt cgcgtaaaat aagattttcc tgctcttttt atttttcatt
    13321 tcaagtccac cttctttcgt gagagcaaga cgaccgctgc aataatgatg aacaatagaa
    13381 tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa ggctgtctga tacaaataaa
    13441 cgcctaagat attgacttta ttcgtcagta gatagacatc tgtaaacata tagaacatcc
    13501 agatagatcg taaaacaatc acagttgcta gtactggttt gattgccggc agtgtcacga
    13561 tcttgaactt ctgccaagca tttgcaccgt ccatttcagc tgcttcataa agcgttttgt
    13621 cgactgtttg caaaatagcc aaaaatgaaa taaacgcata cggatagtat ctccagatag
    13681 caaacaaaac gaccataaag aagctagcaa ctggctggtc gaaccacagc ggtgcttcgt
    13741 tgaaaagctg caacctatct actaacagat cattgacgat tccataacta ttattgaaca
    13801 tatatttcca agcaaaaatc aaagaaatag atggcgtaac ataacttaat aaaatcagtg
    13861 aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa aatagctacg gctaatccta
    13921 aagctgtact gcctgctaca actaagaatg tgtaagcaac tgttaaacct aaagaacgat
    13981 aaaattcact atcacttaaa atttcaagat aattgcttaa tccgataaaa acggcgtctg
    14041 agttaggatt cagcggcata tcgaaaaaac tgatctgaat attggaaagc attggaatca
    14101 gcactaaaac agctaataaa agcatgcttg gtgctaaaag tcccatggct aattttaagt
    14161 cagaatgttt ccttggacga ttcattgaaa cctccccctg ataatctgcc aatagctatc
    14221 tacttttttc gaacaccatt ggcagaactt cttgttattt cgtatttttt tgtgcgtcag
    14281 ctaaagcttg atcgacatct tcgtttccga cagttacttg gttcactgct tttccgatca
    14341 cacctgagct tgtgatatct cccattttcg tgaaattctt ttccccgact agtccaaaga
    14401 cttgtacttc atcaaaagca ctagcaatct cagatggcag ctcgccaaaa gacttgatga
    14461 cttcattttc tttgtaacca tccagttcga ctacttgttt attcactggt tgagcaccac
    14521 caggagacat caagacccac ttttccatgt ttttcggttg agataagaaa gctgtgaatg
    14581 cttcagcagc ctctttttgt ttctcgtcta gaccagaaga aatcgtcaat ccagatactg
    14641 tgccatagac agcttgatct ttttgtgttg ggatagcaaa accgatattt tctgagttcc
    14701 ccgcttcata aacagaaggc aaaatgtaag ttgaatagat tgccattggt gctgttccat
    14761 tcatgaaagc atctttgatt tctgtcacat cattagagcc tggcatcgtg tattgggata
    14821 agttttgata aaacgacaat gcttctctca tcttttccgt atcgatcgtt acctcgcctt
    14881 catcatccaa aacatttgct ccatttgaca aagcaaattg agagaacgct tgctcactca
    14941 ttgttccttc agcagtaggc atagcaattc catattgttt attctcttta tctgtgaaat
    15001 gttgagcaat ttttaaaata tcatcccaat tttctggttc tgaaaaacca gcttctgata
    15061 atttttcttt gtcataccaa ataccttgaa cccagccgct aattggtgca gcaatatagc
    15121 tttttccatc ttctgaacga acaaggtttt tggctccgtc ataataatta tcttctccag
    15181 cttcctcgat aacgttttgg actgccgtct gatcaatcag ttcatcttta tccatcactt
    15241 tagcaaaatc ctgactgact tccattactt ctggtaattt accggaacga gctaacgtaa
    15301 cgatttttgt attgaacgct gattcttcta ccggcacttg cttgattttg atgtctggat
    15361 tttctttttc aaaatcagca accagttcat tgatcacact taatcgttcc tgttcgacag
    15421 aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt agagctttga ctttcaccac
    15481 aagcagtaag aagaaatcca gtagctagca gacaggtagt aacaataatt tttttcattt
    15541 ttttccacct cttattttat tattttcagc cacatatatt catacggctg gaatatcaac
    15601 tttcgttctt gactgcaatg gataatttgt ccagaaaaca ggtctaggta atctcctttt
    15661 ggtaaaagat aacctgctga ttgattggaa aaattatgga taactaagac tttctcttcc
    15721 ttcgcttcgc gaataaaact gataaaagaa tctgctgagt ccagtaagta aatcaaacta
    15781 ttcggatgga aggctttttc tttttttcga atagatagca tctgggtcaa ttcaccatat
    15841 actgctgttc ttaatggatc tgtctgcaac tctttctcaa tatctgctaa caaaaatttt
    15901 tgccgattga tttttcgatt ctctcctgtt ttttctacgc ctttgtaatc atttcgactt
    15961 cccagcacac tttgaatata aattgccggc acgcctggta acgttaccaa aaatgaatga
    16021 gcagctaaaa agcgtttgct tctccattcc gtcgtatctg ttggagaact taacgcatct
    16081 agatacgtag cgtttatctc atatggactg gtcgttccat ctgggttttt cttgtagttt
    16141 actcgggcac cctctttctt cagctgttcc actaattcaa ggatcgtttt ttccggcaga
    16201 atacctcgta ccggattcaa tccgataccg tcatgggagg ctaaaaaatt aaaaaaagtt
    16261 gtttttttcg atgcgaaatc gatggttttt aaccattctt tcaacttact tgtattaccc
    16321 gaacgaatcg cttgaagggt caaaggtggt agcgaaaact gatagaccat ctgtgcttca
    16381 ttctcgccat tcccaaaata actgatatta tctttgtgtg gcacatttgt ctcagtgatc
    16441 aataccgttc ctggagctgc ttcatcagcg attgcacgga ataattggat gattttatgc
    16501 gtttctggca ggtgtataca agaagttcct agttctttcc ataaaaatcc cacagcgtcc
    16561 aagcgaataa atgaagcgcc tttttctaag taaaacaaaa gaatatcgat cattttcaat
    16621 aaaacttctg gatttttaaa attcaagtct acttgatctt cgctaaaagt cgtccatacg
    16681 tgtttttctt tcccgttttt gtcaatgaac gttgtaagta acgggctggt ccttggtcta
    16741 gtcacgctgc ttgtatctgt atccagtggc aaatcaataa aaaaatcttt gtacgtatca
    16801 ttcaagttta aatattcctg aaaccaagta ctttcggcag acatatgatt gcagacaaag
    16861 tcgaacatta accgtgtttc gcttctcatt tcttctatat cttcccatgt gcccaaacgg
    16921 tcgttgattg cctgataatt tacaacggag aaaccatcat cagatgtata tgggaaaaat
    16981 ggcaagaaat gtacaactct aaatgtatct gccaaatact tttgataaaa tttcttaaat
    17041 gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc cataagtaat caagaaaata
    17101 tcttcttcgt cccaatgcag tttgcctgcc tggcctttct tctgctgata aacactgact
    17161 ttttgttcaa tcatagaggc ggttttctca gacaaatctg agtcgcgata aatcgcttgt
    17221 aaaagttttg tatacatcgg attccccttt cttttttggg agcgttccca acgatttgat
    17281 catattatgg aaaacgctta ctgtcaatag cattttttta aataattttg atatacacaa
    17341 aaatacaaat aagaaagctt atgtatcaat aattaatagc ttcttaaaaa aatatggtct
    17401 ctcatgatca tgcaagctat tcctaaattt tcaaaatcat agacaaaaaa gaatgtctat
    17461 tgtactatgg aattattcta aaacgctccc aaaaaggagg tgcatataaa attgaaacct
    17521 actatttatg acattgcgaa aaaagctaat gtctcaaaat caactgtctc cagagtatta
    17581 aataaccagc caaatatctc tgataaagca aaagcaagtg tacttcaagc aatcaaagaa
    17641 ttgaattatg aaccgagcaa aattgcaaga ggactgtcag gaaatggttt tgacgcgatt
    17701 ctagcattat ctcatcgtac ttctcatacg acctatggca atcctttttt ttcagatgtt
    17761 attcaatcca tttctgccgt ttctgaacaa aataacttcg acttgattct acaaacggca
    17821 aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga tccaagaaaa aatgatccga
    17881 ggaattatta ttttaagctc tacgatggat gaaacacttc tttttgaatt ggatcaatat
    17941 aatattccta ttgtggtaat cggaaaagtc ggaaacgact acacgaacat ctactcagtg
    18001 gacacggata atttccagga tagttacgat ttggtggaaa cactgatcaa gaatggacac
    18061 cgtcatatcg gctgtctcca tgctccgaca gaagtttatg tatccaatga tcgtctcaat
    18121 ggttaccgtc aatgcttatt tgatcaccac ctagatatcc gtaatgaatg gattatagac
    18181 ggcggctttc gcttagaaga tagcatggca gcaatgaatg atttaatgaa gctacgagaa
    18241 agaccaactg cgattttttc taccgatgca ttaaaatcat tgagtattta caaagtggca
    18301 gaacaatctg gaaagcagat acctgatgat ctttcgatcg caggattcag tgatccctta
    18361 ttgtcctccc tattttcgcc acagttgact cggatagaca ttccgacaaa tcagttagga
    18421 atcaaagcca cagaactttt attccaacta atccaccacc aataccctga agaaaaaaga
    18481 atcattcttt caaccgagat tactctcaca gattcgatcc aacaacaaga aatgtagaac
    18541 agaattataa aagaacagaa acactgagga taagtcagtt actctgttct tttttcaatc
    18601 actcatccct cgttaccccc tcccggtatt ttagcgaatt tttcttattt tccatctgtt
    18661 ttagatagct gtggataact ataatgtttg tgttaaacta ttttttccac agggaagaaa
    18721 aaactatttt taaccacgtt gtgtataagt ttttatagtt cgttttttta aacacattta
    18781 cccttatttc aatccatgtt atttggggat acttatctct aaaacataaa tagaataaca
    18841 gacatgtcta ctggttttgt tttattcgag gaattattca ataatagcta gcaaaatatt
    18901 aaaaataacc gctctatctt cccatcaatt tattttgcat tcaaaaaatg atctatcttt
    18961 acttttgttg attgacttct cttggaagat gatcgttccg ataccttaat tggatacgaa
    19021 ccgatgttgt taagatcctt tcccactttt cttctagtta gacaaattat tttagacaaa
    19081 ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt ttttaaaata tctattttgt
    19141 ttgattgttc attgcgtctt ctgcagccat ttgcgcttca atatctgaaa tactgtagac
    19201 attttcgctt gctacaccga cttcttttgg ttccatgtca cgataacgtt gcataccagt
    19261 accagctggg atgatcttac ctataataac attttctttc aaaccaagaa gaggatcttt
    19321 cttaccgcgg atcgcagcat ccgtcaagac acgagttgtt tcttggaatg atgcagcaga
    19381 taagaaacta tttgtttcta atgatgcttt tgtgatacct agtaagacag gtcgagaagt
    19441 tgcaggaacg cctcctgaga ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc
    19501 taataatgta cctggtaaga tatctgtatc acctggatcc attacacgaa ctttacgcag
    19561 catttgacga accattactt cgatatgctt gtcgccgatt tctacccctt gcatacggta
    19621 aacacgttgt acttcacgaa gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa
    19681 caattgtttt ggatcgattg atccttctgt caatggtgca ccacggtgga tcatatcgcc
    19741 ttctgctact ttcatacgtg ctgtataagg aacagtatac gtacgtgtat ctgtttttcc
    19801 tttgattgtt acttctttgg tacgtgttgc aggatcttct gagatatcga tgacatcccc
    19861 tgttacttct gtgatcactg cttgcccttt cggattacgt gcttcaaaga tttcttggac
    19921 acgaggaaga ccttgagtga tatcgtctcc ggcaacccca cctgtatgga atgtacgcat
    19981 cgtcaactga gtaccaggtt caccgattga ttgagcggcg atcgtaccaa ctgcttctcc
    20041 gacttcaacg tcagagccag ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg
    20101 ttttgtatta catgtgaaga cagaacggat tgtcactgtt tcgacacctg catcaacgat
    20161 ttgttttgcg atgtcttcgg aaatcaattg gtttcctgca atgatcaatt catttgtttc
    20221 aggatgacgt acttctttac gggtgtaacg tcccactaaa cgatcttcta atgattcgat
    20281 gatttcattt ccctcgcgga tggcttggat ttcaagacct cggtcagtac cgcaatcttc
    20341 ttcacggatg attacatctt gggccacgtc aaccagacga cgagtcaagt aacctgaatc
    20401 ggcagtcttc aaggctgtat cggtcatccc tttacgagcc ccgtgagttg agataaacat
    20461 ttccaagacg gatagtcctt cacggaagtt cgaaatgatt ggcaattcca tgattcgacc
    20521 attaggagcg gccatcaaac cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc
    20581 acgagctcca gaatcagaca tcatgaagat tggatttttc gcttccaaac cttccatcag
    20641 tttttgttgg atgctgtctt ttgcagcatt ccaaacagca atgacacgtt cataacgttc
    20701 gtcatctgtg atcagaccac gacggaattg ttttgtaata gtttcaactt gtttatgggc
    20761 attttcaatg atttcttgtt tttcattcag tacactgatg tcagcgatcc ctactgtgat
    20821 accggcatgt gtcgaatgtt tgtaccctaa gtctttcata cggtcaagca tcttagaagt
    20881 ttctgttact ttgaatcgtt tgaagacttc tgcgatgatg tttcctaagt ttttcttctt
    20941 gaacggtaat actaattctt gctctttgat atgtgcagga atatcagtac cagcttcaac
    21001 aaagtatttg tccggtgttt ggacggtcaa gttgaagtct gttggttcat tcagataagg
    21061 gaattcagga ggcatgatct cgttgaagat gatcttacca actgttgtga tcatcgtacg
    21121 ttctttttgc cattcagtaa atggtttttc acctaatgag ttagggttca caccgatacg
    21181 tgaatgtaaa tgaacatatc cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa
    21241 gaccattcct tcgccttcgc gtccttcttc ttccattgtc aagtagtagt tacctagaac
    21301 catatcttga gaaggagtaa caactggttt accatcttta gggttcaaga tgttttgagc
    21361 agctaacatc aacatacgtg cttctgcttg tgcttcttca ttcaatggaa cgtggacagc
    21421 catttggtct ccatcgaaat cggcattata agcttcacat acaagtggat gcaaacggat
    21481 tgcgcgtcct tgtactaaaa ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg
    21541 tgcccggttc agcaatactg ggtgttcctt gattacgtct tctaaaacat cccatacttc
    21601 gtcttcttga cgttcgattt tacgtttcgc atttttgatg tttgaagcta gttcgcgttg
    21661 aacaagctca cgcatcacga atggtttgaa taattcaatg gccatttctt ttggaagtcc
    21721 acattggtac atcttcaaga aaggtccaac aacgataacg gaacgacctg agtagtcgac
    21781 acgtttacct agcaagtttt gacggaaacg accttgtttc cctttcaaca tatgagaaag
    21841 agatttcaat gggcggttac ctggtccagt aactggacgt ccgcgacgac cgttatcgat
    21901 caatgcatct actgcttctt gcagcatacg tttttcattt tgaacgatga ttccaggtgc
    21961 attcaagtct aacaaacgtt tcaaacggtt attacggttg attacacgac ggtaaagatc
    22021 attcaagtca cttgttgcaa aacgtccacc ttctaattga accattgggc gtaaatctgg
    22081 tggaataaca gggataacgt ccataaccat ccagcttggt tgatttcctg aagcgcggaa
    22141 tgcttctaaa atatccaaac ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt
    22201 caattcttct ttcaattcgg ctgcttctgc atctagatca acattatcaa gtaattgttt
    22261 aatcgcttct gcacccatag cagcttggaa ttcttgtcca tattgttcac gtttttcgcg
    22321 atattcgcgt tctgtcaaca attgtttttt ctctaaattc gtatttcctg gttcgatgac
    22381 tacatatgaa gcaaaataaa tgatttcttc caaagcgcgt gggctcatgt ctaatacaag
    22441 acccatacgt gaaggaatcc ctttaaagta ccagatatgt gaaacaggag ctgctaattc
    22501 aatatgtccc atgcgttcac ggcgaacttt cgaacgagta acttccactc cgcatcgatc
    22561 acaaacgatt cctttataac ggatacgttt gtattttccg caggcacatt cccagtcttt
    22621 tgtaggacca aaaatccgct cacagaacaa cccttcacgt tcaggtttta aggtacgata
    22681 gttaatcgtc tcaggttttt ttacttcacc gtaagaccag cttctgattt tttcgggaga
    22741 agccaaacct atttgcatac tttcgaattt atttacatcg atcaaaaggg gttccctcca
    22801 tttcatttaa tggcaaaccg caagacggct gatcttcgac cgccttgcag ctcatagacc
    22861 ggattagtct aaattatctt ctgcggtttc aaagttttgg atgacatcgt ctttttcatc
    22921 ttccacttgt tcagcagctt tcttttctaa ttctttggca gtttgctgtt cggcaaattt
    22981 tgtcaaagca tcaacagtga tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat
    23041 ctctgtttct tcgatatcca agacacgcat gtccagacct agtgattgca attctttcac
    23101 caatacgcgg aatgattccg gtacacctgg ttttggaatt ggttcaccct tgacgattgc
    23161 ttcgtatgtt ttcacacgtc cgactacgtc atctgatttg tatgtcaaga tttcttgtaa
    23221 agtataagcc gcaccgtaag cttccagtgc ccatacttcc atttcaccaa aacgttgtcc
    23281 accaaattga gctttacctc ccaatggttg ttgtgtaacc aatgagtaag gtccgattga
    23341 acgtgcatgc aatttgtcat cgaccatgtg agcaagtttg atcatgtaca tcacaccgac
    23401 agacacacgt ccatcaaatg gttcacctgt acgtccgtcg tacagaacag ttttcgcatc
    23461 gctggccata ccggcttcac gaactgtttc ccatacgtct tcatcacttg caccatcaaa
    23521 tactggcgtt gctacgtgga tacctaactg acgtgcagcc atacccaagt gtaattccaa
    23581 tacttgcccg atgttcatac gtgaaggcac ccctaaagga ttcagcatga tatcgattgg
    23641 tgttccatca ggtaggaatg gcatatcttc ttccggcata atacgggata caaccccttt
    23701 atttccgtga cgaccggcca ttttatcccc ttcatggatt ttacgttttt gaacgatata
    23761 aacacgaact aacatgttta cgcctggtga caattcatct ccagcttcac gagtaaagat
    23821 tttcacatcg tgaacgatac cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac
    23881 ttcacgagct ttttcgccaa agatcgcatg caatagacgt tcttctgcag ataattctgt
    23941 tacccctttt ggcgtgactt tccctacaag caaatcgcca tcttggactt ctgcaccaat
    24001 acggatgata cccatttcgt ctaaatcttt taatgcgtct tccccgacat tagggatttc
    24061 gcgtgtgatt tcttcaggtc ctaattttgt atcgcgggct tctgattcat attcttcaat
    24121 atggatagaa gtgtatacgt catcttttac caaacgacgg ctcatgatga tcgcatcttc
    24181 atagttgtac ccttcccaag tcatgaaacc aactaaaacg ttttggccta gagccatttc
    24241 gccttgttcc atagatggtc cgtctgccag agtatcccct ttttcgactt tctcacccaa
    24301 atgaacgatt ggacgttggt tgtagcttgt tccggagttt gaacgacgga attttgttac
    24361 atcgtattta tctaatgcac cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta
    24421 ttcaacgaca ccgtcatgtt tacataacaa agcagctcct gagtcatgcg ctgatttgta
    24481 ttccatacca gtaccaaccc atggtgcttg aggattgatc aacggcactg cttgacgctg
    24541 catgttggca cccatcaagg cacggttgga gtcatcgttt tccaagaaag gaatacatgc
    24601 tgtcgcaacg gcaactactt gtttaggtga tacgtccatg tagtctactt tatcaataga
    24661 tacttctaag ttttcacttt gagcacgagc cataacaacg tcttgagcaa atgttccatc
    24721 ttcattcaat gggctgtttg cttgggctac gatgtaatgg tcttcgatat cagctgtcaa
    24781 gtagtcgatt tggtcagtaa cacgacctgt ttcacggtct acacgacgat aaggtgtttc
    24841 gataaatccg aatttattta ctttcgcata actggacaaa ctattgatca acccgatgtt
    24901 tgggccttca ggagtttcga tcggacacat acggccatag tgggagtagt gaacgtcacg
    24961 aacttcataa ccggcgcggt cacgagtcaa tccgccaggt cctaaggctg aaagacgacg
    25021 tttgtgtgtc aattcgccaa gagggtttgt ttgatccatg aactgtgaca actgagaaga
    25081 accaaagaat tctttgatac ttgccacaac tggacggata ttgatcaatt gttgaggtgt
    25141 caatgtttca gtatcttgga tcgacattct ttcacgaacc acacgttcca tacgtgctag
    25201 cccgatacgg aattggtttt gtaaaagttc accgactgaa cggatacgac gatttcctaa
    25261 gtggtcgata tcatcgacat tgccgatacc ttccatcaag ttcaagaaat agctcattga
    25321 agcaatgatg tcggctggac gaaccgtttt tacagctgct tctggatagc cgtttccgat
    25381 cacgtttact tcacgttcag ggtctcttgg tgaaaatact ttgatgactt ggacagtcat
    25441 tggatctgtg accacaccgt cttctgatgg atagtaagtc actgaattta ggccattatc
    25501 aatgaaagga gctagtgttt ccatgacttg gtgagtcaga actgttcctt tttcaacgat
    25561 gatctcgcct gtttctggat caactaatgt ttcagctaat gtcagattca atagacgtgt
    25621 tttcaaatct aattttttgt ttactttgta acgaccaacg tttgctaagt cgtaacgttt
    25681 tggatcgaag aaacgagcat tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc
    25741 tggacgcaaa cgttcgtaaa cgtctttcaa accttcttct gttcttgagt cgcttgcgtt
    25801 tttatgcaag tctttttcga ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa
    25861 gatcgtatca tcggaaccaa atcctaacgc acgaactaat acggttaaag gaatcttacg
    25921 tgtacggtcg atacgtacat aagagatgtc ttttgcatca gtttccattt ctagccaagc
    25981 tccacggtta gggataacag tagaaccgaa tccttctttt ccgtttttgt cgacttttcc
    26041 atggaaatag acacctggtg aacggactaa ctgagaaacg attactcgct ctgccccgtt
    26101 gatgatgaat gttccttgtt ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc
    26161 tttgatttcg ccagtctcgc ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta
    26221 gtttgcatca tgcgcacgag cttctgctac tgtatacttt ggttctttca attcataatc
    26281 tacaaattcc aaagatagat tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat
    26341 ttctctaagt ccctcatcta agaaccattg gtaagagtct gtttgaattt cgatcaaatt
    26401 tggtaattcc aacacttcac tgattcttgc gaaacttcta cgttcgcgat gttttccgta
    26461 ttttactacg tgtccagcca agctcttcac ccctctaaaa tgtcattcaa aaagactacc
    26521 cgatcaattg ttacaataat atttcaaata ttatttttag gtaacaacag acattttttg
    26581 cggcttttag gcaaaaaaaa accaaaaata gaacattctc ttgaaacctt acttcgtgag
    26641 cttctacttt tgtttcctta tattaaagcc gaaacggaca atatttcgtt tctgcctttt
    26701 cttcatcaga tagtttttgc aacttcatat actacactta ttttctataa aagtcaacac
    26761 ttcctggcgt ttcttctgct ttttcgcaac attttcttaa tgttttcaat tttatgtttt
    26821 caattatctg acaattctta attgacagac ctttttatct cggttatact ttagaaaatt
    26881 cattttgtgg aggaacttct atgcgaacca acatcaacca aatcgcacca acacaactga
    26941 ataactttta ctttagctac tttagatagg tttgtattca tcatgatgga tacaaacgtt
    27001 aaacgacagt ttgtatctat gatggctaaa gaactttgta ttgcagcaaa aagccacgta
    27061 gacaaaatgc taagtggctg agcagtgaaa atacaaagta aaagtatcaa gcattctact
    27121 ttttcaattt attttccgac ttttcaatcc atttggacat tttgtctgaa tggatttttt
    27181 gtttcccaaa attaaggagg aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc
    27241 tgccctgcta ctagctttag gaggttgcgc acaaaaagac aattccgcag aggcatcaga
    27301 tggacaaaaa acaattggcg tattgcaact tgtacaacat aactcgttag atgctgccta
    27361 taaaggattc aaagaaggat tagcagaaga agggtataaa gaaggcgata atctaacaat
    27421 cgattatcaa aatgcccaaa acaatcaaga caacctgaaa agtatgagtg agaaactcgt
    27481 caaagaagat cctgatttgc tacttggaat cgccactcca gcagcacaat ctttattaaa
    27541 tgaaacaacc actatcccga tgactgtcac agcggtcaca gacttggaac aagcccattt
    27601 agtcacatcc aatgaaaaac ctggcggcaa tgtgactggc acgacagata tggtgccaat
    27661 cgaaaaacaa atcggcttac tgttaaatat cgtgccagac gccaaaacaa tcggcatcat
    27721 gtataacaac ggtgaagcaa actctaagat ccaagctgat ttggcagaaa aagaattgaa
    27781 aaaagcgggc gtcaaagtca aagtactgac agcaaattcc acaaatgatg tccaacaagt
    27841 gacgaaaagt ctagctaaag atgtagatgg aatctatatt cctactgata acacgttcgc
    27901 aagtgcggca agtgttgtcg gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg
    27961 cgcagttgat caagtcaaag acggcggtct agcaacagta ggaatcgact acgaaagctt
    28021 gggcaaacaa acagggatca tggcagcaaa aatcttagac ggcaagaatc caagtgaact
    28081 tcctgtcgaa acagcgaatg atttggaact gtatgtcaat aaagacatgg ccaaagcatt
    28141 aggcatcaat ccagacagca tcaaagaagc tgaataaaac acaggaggat tctcatgaca
    28201 aatttgctct ctcttttctt atcatctgca tcgcaagggg tcttatgggc cctacttgcg
    28261 attggtgtat acgtcacttt ccgtatcttg gacattgccg acttgactgc tgaaggaagc
    28321 tttcctttag gtgcagggat cgcggcagta agtattacaa atggctatca tccattgaca
    28381 gcttgtcttc taggcttttt agggggtgcg gcagcagggc ttgtttccgg actgcttcac
    28441 acaaaattga aaattcccgc ccttcttgca gggatcatca ccatgactgg tttatattct
    28501 gtcacatccc gtgtgatggg tgcgccgaac atctctttat tgggtcagcc aacgatcttt
    28561 cattgggtcg agtcattcgg cataacaaaa gacaacgctg tactgatcgt cggtctgctt
    28621 tttgctttga tcgttgtatg tttacttgtt ttgttcttac gtacagaaac aggacttgct
    28681 attcgtgcaa ctggcgataa tctggcaatg agcgaagcaa acgggatcaa cacagataat
    28741 atgaagatca tcggttatat gatttctaat ggatgcattg ctctttctgg ctccttactt
    28801 gcgcaaaaca acggctttgc tgatttaaat tctggtattg gaacgatcgt aattggactt
    28861 gcttctatta tcatcgcaga agttttgtta cgtaaccagc cactatttct ccgtctcttg
    28921 actgtcgttt taggtgcggt catttatcgt tttattttag cgttagtctt cgaactgaat
    28981 gttgaaccaa gtgattcgaa acttgcttct gctttcgtgc tagtgatctg tctttctttc
    29041 ccgcagatcc agcaaaaatt ccatttacct caaaaaaaga aaggcggtag tgtcaaatga
    29101 atgacgtctt aacattggaa aatctccatc atacctttga aaaaggaacg atcaacgaaa
    29161 atcatgtcct gaaaggcatt aatctttcct tagatcccgg cgacttcgtg acgatcatcg
    29221 gtggaaatgg ggctggtaaa tctactttgc tgaacagcat cgccggtaca ttttctgtcg
    29281 atcagggtca aattttattg aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt
    29341 cgaaatccat cagtcgcgta ttccaagatc ctaaactagg gacagccgtt cgtttaacgg
    29401 tagaagaaaa tcttgctctt gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg
    29461 tcaaaccaca agaccgaaca tttttcaaag accagctggt tcggctaaat ttggggttgg
    29521 aaaatcgttt atcctcagaa atcggtttgc tgtctggagg acaacgtcaa gctattacgt
    29581 tattgatggc cacattacag cgtccagaac tgatcttatt agatgaacac actgcagcgc
    29641 ttgaccctgc tacttccaaa acggtgatgg aattgacaga aaagctgatc actgagcaga
    29701 atctcactgc ttttatggtc actcatgaca tggaagacgc gatccgctat gggagtcgac
    29761 tgatcatgct gcatcaagga aggatcgtcg tagatatcca gcaagaggaa aaacagcagc
    29821 tcacagtgcc ccaattatta gcgttgttca aacaaaacag cggcacggca ttaaaagacg
    29881 atcaagtact tttatcttca taatcagaaa gaggatggga caaaaaatga acgcccaccc
    29941 tcttcttttt tctctttcct tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg
    30001 aaaactattt tgttctctcc ttcttcctga tagaatggat gggaaaggag cttgatgcca
    30061 tgacaacgaa agacaaggta cttcaattat tgaaaaagac accagatttt ctttccggag
    30121 aaaagttggc acagcaactg gaagtttccc ggacaagtat ttggaaagcc atcaaagaat
    30181 tggaaaaggc aggctatcgt tttgagcacg ggcctacagg gtatcgttac ctgccctctg
    30241 acgtactaga tgcaaaagaa atccagcaag agctacggtc gtttattcct gatttgaaag
    30301 tacaaacgat gttgacttct gaatctacga tgaaagatgc caaactggca gcagctcaag
    30361 gagattctgt ttcgaagtta tttgttgctg atacacaaga acaagctcgt ggacgttttg
    30421 gacggccttt tttcgctgaa cctggacgag gaatctacat gagtctcttg ctccatccag
    30481 acaaacggtt tgatgaattg cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag
    30541 ccattgaatc tgtaaccggt cgttctgctt cgatcaaatg ggtcaatgat atctacatgg
    30601 atggaaaaaa agtctgtggg attttatctg aagcagtcag tgatatggaa agcggcagga
    30661 tcagtcatat catcattggg atcgggctta atttttctgt tcctcaaaat cattttccag
    30721 ctgatctcca agaaaaagcc atctctgtct ttcccgacgg acaagctagt gtcacaagga
    30781 atcagctaat cagcaaaatc tggcaagaat tgttcactta tctttctcac ccagaaaaag
    30841 cgttggactt ctataaagaa aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa
    30901 aaggaacgac ttatgtcggt cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc
    30961 aaacaaatga acagaagatg agtttgtctt ctggcgaaat cagtctcaca tctatccgta
    31021 cgcaataatt atcagatttc acaaaaggac acctgatctc atacagtgtc cttttttctg
    31081 aattctcttc aaaagactct ccttattcta tcagctgaca gtttctctat cctctagaaa
    31141 aaggtattct aaaaatatct ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa
    31201 aaacaagaga gtcatcagtt tacacgatca aaagagatag caggccgctt cctttttcaa
    31261 tcttatagat agaccatggg taacgataaa aacaaaaaag aacttgcgac ttattttgcc
    31321 gtaagttctt ttttagctag tgccttaatc tcttaatccc aacaaacgtc tcgcatagat
    31381 cacctgacca gagtgcatag cagcatcatc aataatagaa acgagacgca ttcctcgtgt
    31441 gactggtggt gtccagttct cgtcaattac ttcatctagc tcggaaggtt ccagccttct
    31501 taagtaagaa atagcaaaat cagcagctcc tcgcaagtat cctagcagat cttctatttg
    31561 attagcttgg atctgctttg cttgttccag actatgcttc caatcttgtt cgttatctgc
    31621 tatcgtcatc ggaaaccggc tttcccagtc ttgactatgc caaattggag aagttcctgc
    31681 taaatcagca atctggaaat ctaattctcg agcggtatgc caagccagcc aagtcattga
    31741 tttgatctgc ggggctctat cttccactgg aaaacgattg gcctcctcaa tagagacgcc
    31801 ttcaaacaaa cgttcaaacc gatcgtatgc ccgatccaca ctctcgcata gcatatctaa
    31861 ataatccatt tattttcctc ctttttattt atcctactta ctttttccta attctaggat
    31921 acctacttat atgttactac acacccttat tttttacact ttaacagtca ccatcatcaa
    31981 tagtttggag attgaaagaa aaagatagtg tcgaactatc aattgaacaa gaagcaacaa
    32041 tatgaacgtt tcattaatat tctttaaata aacaatgatt tttcaaacga acagtttaag
    32101 actaggagaa aaagagtata ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa
    32161 aaaaagggca agtttactag tcctagttgc tttgttgttc aatatctttt cgtatggtgt
    32221 ggccgtttca gcggaaacat acattcctaa agaccctggg aatatttata atgatctaac
    32281 tggtaactat ggcttgttag gaattgctag tcaattccac gtatttgcta aaaataagac
    32341 aactgtgaat gcacatacag acggaaatat agcaacacat gaacttgatg caaaaaataa
    32401 ttttggtaca gagcttcatg aaggtttatt gaaccaagaa ataaattata tacaaaaaac
    32461 tgattctttc gctaattctt caggcatacc tacaggtagc acaagaatga ataaatttgt
    32521 cgttggcagt aacgtggatg tagcacttca agatggtcat cctacagtca atggatccag
    32581 gcttgatcac ttaactccag atgattttta taaagatcgt gctgggcatg aatatattga
    32641 tttcgagcag gaatttgcta aattgaatgt agcagcaaat gatcttgcaa cgatcactcc
    32701 agctaaaaca tatactgctg cagattttcc agacatgaac aatcggacaa tcgatttaac
    32761 tgatttatca gataccgggt ttttattggt aaatatcgat gcagaagtgt taacgatgaa
    32821 tacgccactt caaatcatta atccaaatga tcaagtagtt gtatttaatg tcatcaacag
    32881 ttcttctgca ttgaacgtac agtcaccaat caaatacaat gatcgttcaa atcatgaaac
    32941 agaagatttt tcggatgcga atatctcttg gaattttgga aatgagatga ctgatttgac
    33001 aatcagtgct ccttttcaag gaacaatcct tgcgcctaat gctactatcc gcattactca
    33061 aaaccaagac ggaacgatta taggaacaaa tgtgatttta gatgcagcta ccaatcgttg
    33121 ggatcctaac gaaatattta tcagcgacaa tggtacagat acgacagaca caagcgacag
    33181 tacagataca tctaccacaa gtgatagctc agattcatca
//
LOCUS       DACDRK010000025        32533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDRK010000025 DACDRK010000000
VERSION     DACDRK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.025"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWJ77_001261"
     CDS             131..505
                     /locus_tag="IWJ77_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9452892.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWJ77_001262"
     CDS             complement(619..1248)
                     /locus_tag="IWJ77_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9452893.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWJ77_001263"
     tRNA            complement(1382..1454)
                     /locus_tag="IWJ77_001263"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IWJ77_001264"
     tRNA            1668..1741
                     /locus_tag="IWJ77_001264"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IWJ77_001265"
     CDS             1696..1845
                     /locus_tag="IWJ77_001265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452894.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IWJ77_001266"
     CDS             2338..3666
                     /locus_tag="IWJ77_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9452895.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IWJ77_001267"
     CDS             3868..4701
                     /locus_tag="IWJ77_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9452896.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IWJ77_001268"
     CDS             4717..5454
                     /locus_tag="IWJ77_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9452897.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IWJ77_001269"
     CDS             complement(5609..6577)
                     /locus_tag="IWJ77_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9452898.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IWJ77_001270"
     CDS             6809..7642
                     /locus_tag="IWJ77_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9452899.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IWJ77_001271"
     CDS             7676..8515
                     /locus_tag="IWJ77_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9452900.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IWJ77_001272"
     CDS             8541..9101
                     /locus_tag="IWJ77_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9452901.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IWJ77_001273"
     CDS             9300..11021
                     /locus_tag="IWJ77_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9452902.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IWJ77_001274"
     CDS             11186..12328
                     /locus_tag="IWJ77_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9452903.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IWJ77_001275"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IWJ77_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9452904.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="IWJ77_001276"
     CDS             14128..14775
                     /locus_tag="IWJ77_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9452905.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15168..17813
                     /locus_tag="IWJ77_001277"
     CDS             15168..17813
                     /locus_tag="IWJ77_001277"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9452906.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="IWJ77_001278"
     CDS             18123..19436
                     /locus_tag="IWJ77_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9452907.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="IWJ77_001279"
     CDS             19426..20079
                     /locus_tag="IWJ77_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9452908.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /locus_tag="IWJ77_001280"
     CDS             20134..20826
                     /locus_tag="IWJ77_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9452909.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="IWJ77_001281"
     CDS             20976..21176
                     /locus_tag="IWJ77_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9452910.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="IWJ77_001282"
     CDS             complement(21430..23109)
                     /locus_tag="IWJ77_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9452911.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="IWJ77_001283"
     CDS             complement(23111..23323)
                     /locus_tag="IWJ77_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9452912.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="IWJ77_001284"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="IWJ77_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9452913.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="IWJ77_001285"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="IWJ77_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9452914.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="IWJ77_001286"
     CDS             27298..27495
                     /locus_tag="IWJ77_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452915.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="IWJ77_001287"
     CDS             27648..28133
                     /locus_tag="IWJ77_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9452916.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IWJ77_001288"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="IWJ77_001288"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9452917.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="IWJ77_001289"
     CDS             29010..29537
                     /locus_tag="IWJ77_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9452918.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="IWJ77_001290"
     CDS             29628..30365
                     /locus_tag="IWJ77_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9452919.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="IWJ77_001291"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="IWJ77_001291"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9452920.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="IWJ77_001292"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="IWJ77_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9452921.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5522 c         6874 g         9365 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DACDRK010000026        31842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDRK010000026 DACDRK010000000
VERSION     DACDRK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.026"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(151..2304)
                     /locus_tag="IWJ77_001293"
     CDS             complement(151..2304)
                     /locus_tag="IWJ77_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9452922.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="IWJ77_001294"
     CDS             complement(2598..3446)
                     /locus_tag="IWJ77_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9452923.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IWJ77_001295"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IWJ77_001295"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9452924.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="IWJ77_001296"
     CDS             complement(5947..6126)
                     /locus_tag="IWJ77_001296"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9452925.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IWJ77_001297"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IWJ77_001297"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9452926.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="IWJ77_001298"
     CDS             8274..10553
                     /locus_tag="IWJ77_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9452927.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="IWJ77_001299"
     CDS             complement(10646..11293)
                     /locus_tag="IWJ77_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452928.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="IWJ77_001300"
     CDS             complement(11472..12191)
                     /locus_tag="IWJ77_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9452929.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="IWJ77_001301"
     CDS             complement(12322..13473)
                     /locus_tag="IWJ77_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9452930.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="IWJ77_001302"
     CDS             complement(13690..14430)
                     /locus_tag="IWJ77_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9452931.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IWJ77_001303"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IWJ77_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9452932.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /locus_tag="IWJ77_001304"
     CDS             complement(14814..15407)
                     /locus_tag="IWJ77_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9452933.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16038)
                     /locus_tag="IWJ77_001305"
     CDS             complement(15391..16038)
                     /locus_tag="IWJ77_001305"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9452934.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IWJ77_001306"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IWJ77_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9452935.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IWJ77_001307"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IWJ77_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9452936.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="IWJ77_001308"
     CDS             complement(17484..18011)
                     /locus_tag="IWJ77_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9452937.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="IWJ77_001309"
     CDS             complement(18191..18976)
                     /locus_tag="IWJ77_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9452938.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="IWJ77_001310"
     CDS             complement(18969..19838)
                     /locus_tag="IWJ77_001310"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9452939.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="IWJ77_001311"
     CDS             complement(19839..21218)
                     /locus_tag="IWJ77_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9452940.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IWJ77_001312"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IWJ77_001312"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9452941.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IWJ77_001313"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IWJ77_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9452942.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IWJ77_001314"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IWJ77_001314"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9452943.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IWJ77_001315"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IWJ77_001315"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9452944.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IWJ77_001316"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IWJ77_001316"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9452945.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="IWJ77_001317"
     CDS             complement(25176..25400)
                     /locus_tag="IWJ77_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9452946.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="IWJ77_001318"
     CDS             complement(25453..26415)
                     /locus_tag="IWJ77_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9452947.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="IWJ77_001319"
     CDS             complement(26425..26865)
                     /locus_tag="IWJ77_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9452948.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="IWJ77_001320"
     CDS             27171..28136
                     /locus_tag="IWJ77_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9452949.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="IWJ77_001321"
     CDS             complement(28193..28423)
                     /locus_tag="IWJ77_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9452950.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="IWJ77_001322"
     CDS             complement(28509..29396)
                     /locus_tag="IWJ77_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9452951.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="IWJ77_001323"
     CDS             complement(29485..30708)
                     /locus_tag="IWJ77_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9452952.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31804)
                     /locus_tag="IWJ77_001324"
     CDS             complement(30725..31804)
                     /locus_tag="IWJ77_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9452953.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8794 a         6970 c         5488 g        10590 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt tt
//
LOCUS       DACDRK010000027        29983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDRK010000027 DACDRK010000000
VERSION     DACDRK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.027"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            139..477
                     /locus_tag="IWJ77_001325"
     CDS             139..477
                     /locus_tag="IWJ77_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452954.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="IWJ77_001326"
     CDS             complement(682..1257)
                     /locus_tag="IWJ77_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9452955.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="IWJ77_001327"
     CDS             complement(1989..2570)
                     /locus_tag="IWJ77_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9452956.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="IWJ77_001328"
     CDS             complement(2753..3379)
                     /locus_tag="IWJ77_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9452957.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="IWJ77_001329"
     CDS             complement(3401..4732)
                     /locus_tag="IWJ77_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9452958.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="IWJ77_001330"
     CDS             complement(4887..5288)
                     /locus_tag="IWJ77_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9452959.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="IWJ77_001331"
     CDS             complement(5374..5580)
                     /locus_tag="IWJ77_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452960.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="IWJ77_001332"
     CDS             complement(5675..7177)
                     /locus_tag="IWJ77_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9452961.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IWJ77_001333"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IWJ77_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9452962.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="IWJ77_001334"
     CDS             complement(7748..8365)
                     /locus_tag="IWJ77_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452963.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="IWJ77_001335"
     CDS             8586..9959
                     /locus_tag="IWJ77_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9452964.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="IWJ77_001336"
     CDS             10034..11146
                     /locus_tag="IWJ77_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9452965.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="IWJ77_001337"
     CDS             complement(11218..12855)
                     /locus_tag="IWJ77_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9452966.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..13631)
                     /locus_tag="IWJ77_001338"
     CDS             complement(13023..13631)
                     /locus_tag="IWJ77_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9452967.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14015..15166)
                     /gene="queG"
                     /locus_tag="IWJ77_001339"
     CDS             complement(14015..15166)
                     /gene="queG"
                     /locus_tag="IWJ77_001339"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9452968.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15185..15937)
                     /locus_tag="IWJ77_001340"
     CDS             complement(15185..15937)
                     /locus_tag="IWJ77_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452969.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16078..17028)
                     /locus_tag="IWJ77_001341"
     CDS             complement(16078..17028)
                     /locus_tag="IWJ77_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9452970.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17029..17868)
                     /locus_tag="IWJ77_001342"
     CDS             complement(17029..17868)
                     /locus_tag="IWJ77_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9452971.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17865..18611)
                     /locus_tag="IWJ77_001343"
     CDS             complement(17865..18611)
                     /locus_tag="IWJ77_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9452972.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18849..20219
                     /locus_tag="IWJ77_001344"
     CDS             18849..20219
                     /locus_tag="IWJ77_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9452973.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20274..21170)
                     /locus_tag="IWJ77_001345"
     CDS             complement(20274..21170)
                     /locus_tag="IWJ77_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9452974.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21451..22128)
                     /locus_tag="IWJ77_001346"
     CDS             complement(21451..22128)
                     /locus_tag="IWJ77_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9452975.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22219..23124)
                     /locus_tag="IWJ77_001347"
     CDS             complement(22219..23124)
                     /locus_tag="IWJ77_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9452976.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23299..24753
                     /locus_tag="IWJ77_001348"
     CDS             23299..24753
                     /locus_tag="IWJ77_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9452977.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24769..25800
                     /locus_tag="IWJ77_001349"
     CDS             24769..25800
                     /locus_tag="IWJ77_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9452978.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25807..26994
                     /locus_tag="IWJ77_001350"
     CDS             25807..26994
                     /locus_tag="IWJ77_001350"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9452979.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27150..28532
                     /locus_tag="IWJ77_001351"
     CDS             27150..28532
                     /locus_tag="IWJ77_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9452980.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28504..28890
                     /locus_tag="IWJ77_001352"
     CDS             28504..28890
                     /locus_tag="IWJ77_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9452981.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29090..29758)
                     /locus_tag="IWJ77_001353"
     CDS             complement(29090..29758)
                     /locus_tag="IWJ77_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9452982.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8404 a         6141 c         5483 g         9955 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta
    13621 aatgaattca tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact
    13681 ttatcattta gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga
    13741 attatttaat atttgtaaca tttaaacaaa tatttttttg atacaaaacc ataaaagcct
    13801 tgttgtataa gcaaagtaat cgtttttcta tgttttttta tttttgaatg aaaaacgcag
    13861 attctgtaac attcactgca ataatccata aaaaaaagca aaaaaatttt tgatttttat
    13921 caaattctct taaaaaaatg taaaaaatac aaaaaaacca gtaattgtta caacaattac
    13981 tggacaaaaa ctttgaataa tgttaactat ttttttattt taactttctc cgcaataatt
    14041 tctgggcatg ttccaattcg tttataacga cttcttctgt ttctttagct aatatttcat
    14101 ctaatacatc aagccatttt tccggttctt ttgttcctag acgtccaata gcccaagctg
    14161 ctgttcctct gatgacaggg cggacatctg ttaaacattc tgaaatctca ggcaatgccg
    14221 ttcgatcacc tagattagct aaagcaatca atgcgtttct ctgcaaaggt ttcttcccac
    14281 gccatgaacc agctaaatgc ccaaattgct gtttgaagtc tttattagac atcgaaagca
    14341 ttggctttaa ttttggatat acttcgtcta cttttggttc catctcttca tgaaaatgaa
    14401 aatcttttcc ttgattgtaa gggcagacta actggcagat atcacatcca taaatgacat
    14461 ttcgcatttt tttacgatat tcttccggca tcatcccttt tgtctgagtt tgataagaca
    14521 aacatcgctg ggcattcatt cttccgtcac caagcaatgc ttttgtcgga caagcagtca
    14581 cgcagcgcat acagtcaccg caaccaaatt ctcctggttt atcaggctca aatacaatat
    14641 tagtaacgat ctcaccaaga tagacaaatg aaccgaactc ttcagtgatc aacaatccgt
    14701 ttcgtccaat aaatcctaaa ccagctcgct gtgctacagc gacatcgatc agttctccag
    14761 tatctacttg cggagctaga cgccattcct cttctttttg ccatttttct gctcttgttt
    14821 ggatgaattc gatcaatcgg ttcaaacggt ctctgagtat atcatggtaa tcgatccccc
    14881 aagaagcacg agcaaactgc ccgcgttttt catccctagg cacttcttca tggatcctag
    14941 taggatacgc aagtgcaatg gaaatgatcg atttcggctg atcaaatgtc atttctggat
    15001 aaagacgctc atcgatattt ggatgttcaa agccagaagt atggccagct gctttttgtt
    15061 cttccaacga ttcttttaat gaatcaaaag gttccgccgt ggtgaacccg attttgtcaa
    15121 ttcctaatcg tttgctttct tgtatgattt cttcttttaa tgtcatggtc ttcctcgctt
    15181 gctgttaacg gctgtataac ccataatata gtttttcttt tttcagctgg acaaactgga
    15241 tctgctgatc ggcaatcttc ttttcttcca tccatttttt tcttgcagca aatacttccc
    15301 aataatttgt tgaaattaat tttcttttgt tatctaatgc attaaaaaaa ttcaatgaaa
    15361 ctggttttgt acttaacagt tcatggagcc ttacctcttc ccatggcgaa aactcagaca
    15421 gatcgcggga atacaatact tcttcataga gatttttttg ctgtgtgtac gtgaatgttt
    15481 gcgggatttt cccaggccta gcaatttcta ctagacgatt ataaaacata ttcattaatt
    15541 tgctgttggc aataaattga cttttataat aggccgactg gatctttttg attactcctt
    15601 ctgctttgat gactgaaaat aatgcaggcc atgctttgac ggttatttcg tcgttttgtt
    15661 taactaatac gccaggtgtg aagccgactg gagaaaggaa cacaaaataa ccgataccgg
    15721 tattttcatc taaaggctga aattcatcaa acaaccattt gcctatgtac agctcttcat
    15781 ctggttctgt tgtctcattt tgatttgcta tgaataattt agctaattct gcttcaagaa
    15841 attgaaggtc ttgatccttg tcggtatttt cctgctggct gatcaatcga aagacttctt
    15901 ctttaatcgt atatttatct ttacgtgtaa aattcatagc cacaccttcc ttccttactc
    15961 taactataac ggaatttctt caaaaaaggt atctgtacga cgaaaaacag cataattttt
    16021 tttcatatct cttataaaaa atgcaaggtc tgctcctcaa gctagacctt gcacttttca
    16081 gctttctttt gtttgtgaca aaccttcatg gatcgtttca atattccatt tcatcatctg
    16141 atagtaagaa tctccttgtt ctccttcttt tgctatggaa tcagtgaaca gtttatcata
    16201 gattttcaaa cccgtttctt ttgctacccg ttccatgctt cttgagtcta cactggtttc
    16261 cacgaataaa accggtactt cttttgctct tatctgatca atgatcgctt tcatttgatc
    16321 aggcgtacct tgactttctg tattgatctc ccatatataa gctgctggca aatcatatgc
    16381 tcgtgaaaaa tacttgaatg ccccttcact tgttactaat aattttttgt tctcttcgat
    16441 agaagcaaaa ctttccttcg cttgtgtatc taattctttt agtttttcca catatgcttc
    16501 tgcatttttt ttatagagtg tcgcattttc tgcatctttt tcagagaata tacgtgcgat
    16561 ttcctccaca tattttattc cgttagatag gtctagccaa gcatggggat ctgcttttgt
    16621 atgttcttca ccgctagtta aataaagagg ttctacattt ttgctaactg caaaataatc
    16681 ttttccttct tcttttttag ccgtttccat caagttatcg aaccagctgt tacctgtttc
    16741 aagattcaaa ccgttgtata aaataacatc tgcatcgctt gcctttttga tgtcttctgg
    16801 tagtacttca tattcatgcg gatctgttcc gactggtacg atactgtgga tatctatttg
    16861 atctgttccg acctcttttg ccatgtccgc caagatcgaa ttggtagcca cgactgacaa
    16921 cttttcttta cttccttcat ccgcttggtc agaagcctgc tttccgcaac cggctaatac
    16981 caagaaacta actgccaaga ttaaaaagag tgattttttt gttttcatct atctgcttcc
    17041 tttctgaaat aaaattcctt ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt
    17101 acatgctaac acgatcgtag ctcctgatgg ccaattgtaa ctataactga aaaacacacc
    17161 gacaactgca cttagcatac cgattcctgc agctgtaatg atcattttca gtaaatggtt
    17221 cgttagcaag taagccgtgg cggctggtgt gatcaacatg gcaatcacca agattgttcc
    17281 tactgtctgt aaactgacta cagccactaa tgtcaaaaag aacatcaaaa gataatggat
    17341 cagcgatgtg ttcagcccat aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa
    17401 ttctttataa aagaaaaaga caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa
    17461 caaatcgctt tccctaacag ctaagacatt cccaaataaa atatgataca aatctgtcgc
    17521 actctgggca tatgaaatca agataatccc taatgcaaaa aaggagctaa agacaattcc
    17581 aatcgctgta tcatttttca atcggctccg ctgagtcacg aaaccaatca ctgctgctgt
    17641 tagcatccca aaagcagtgg ctccaaagat gtaactaaaa ccaaacatat aagaaatggc
    17701 tacacctgga agaactgcgt gagagatagc gtctcccatc aaagacatcc ctcgtaaaat
    17761 aataaaagaa ccgatgactc cagaagcaag tcctacaagc atggaagtga ccaaagcgta
    17821 ttgcaggaat tgataattca ttaatccatc aataaaggat tggatcattt tttctctcct
    17881 ccttttacta aatgtctgat gatctcacca taggccaatt gaaggttctt cgatgtaaag
    17941 acttcttgta ctggaccgga agcgatcagc tgtttattta aaagaataac ctcatcgaaa
    18001 tattcttcta ctttatgaag atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc
    18061 aattcctgca agatgtcaaa gatctttcgt tcacttaacg catcaatccc tacgaatggt
    18121 tcatctaaaa agatccattc tgctccttgg gctagagctc ttgcaataaa aactctctga
    18181 agctgtccac ccgatagttc gctgatctgt cttttggcat attcttccat ccctactttt
    18241 ttcaaagctt gtcttgcacg ttctttttct tttttcccag gtctttgtcc aattcgtaaa
    18301 gatggatatg ttcctaaaag tacaacgcct aatacatcaa ttggaaagga aagatccaat
    18361 tcacttcgct gttccacgta ggctattttt ttacggactg agtttaccgg ctgtccagaa
    18421 aaggtgactt taccagtagt agctggtatc aactctagca aagatttcat aaatgttgat
    18481 tttcctgcac cgtttggtcc gataataccg gtaatttttt gtggatttat cgtcaagttg
    18541 atagttttca aaacagtttt cccttgataa gcggctgaaa ggtcttgaat tacaatggcc
    18601 gtacgttcca aatcttacac tcctttttag gtttacctaa ctttttattt aaatatagtt
    18661 taactaaaat ggaaagcatt ttcaagtaga atgatgaaaa agtttttttc gaattttcct
    18721 cgcattttat gaaaattttc tgtagaatca catcaagagt tcaataaggc taacctaata
    18781 atgaaaaaca tgtgttgaaa cgtttgatta ttttttcttg ttgactcaac aacgaaagga
    18841 gtttttgtat ggaagaagaa aagttaactc gaagtcttag tgcccgtcat atccaaatga
    18901 tcgcactagg tggaacgatc ggtgtcggtc tgtttatggg tgcctcctca accatacgtt
    18961 ggacagggcc ctcagtgatg ggagcctatg cgattgcagg attctttttg tatttgatta
    19021 tgcgtgcttt aggcgaaatg ctttacgttg atccttcgac tggttctttt gcaaattacg
    19081 cgagcgagta catccatcct gtggctggct acttgaccgc ttggagtaat atttttcaat
    19141 atattgtcgt aggaataagt gaagtgattg ctgtaggatc ttatatgaat tattggtggc
    19201 ctgatttgcc ggcaattatt cccggaatca ttgtcgtctt gtttcttatg ctggcaaatc
    19261 ttatttctgt caaggcgttt ggcgaattag aattttggtt ttcgatcatc aaagtcataa
    19321 cgatcatttt gatgatcatt gcaggtcttg gggtaatctt atttggattt ggtaatcatg
    19381 gtcaggcagt agggatttca aatctttgga aaaatggtgg attcttcact ggaggcgtca
    19441 aaggattttt ctttgcatta tcgatcgttg ttgcttccta tcaaggaatc gaactgatcg
    19501 gaatgactgc tggtgaagca gagaatccta aaaagacgat cattgaagct gtccagtcca
    19561 ctatcggtcg aatcttgatt ttttatattg gtgctatttt tatcattgtg agtatctatc
    19621 cgtggaatca attgagtgag attggttcac cttttgtaca aactttttct aaagtcggca
    19681 ttacatttgc tgcaggactg attaattttg tcgtgattac agcggcatta tcaggttgca
    19741 attcagggat tttcagcgca agtcgtatgg tctatacact tgccataaat ggaaagatgt
    19801 ctaaaaaatt tctgaaacta tctcgtcacg gtgcgccttt ttatcctgtc gtagcaatct
    19861 caggtggaat cttaattggt ctgatcttaa attatcttct accaatgttc atcaaccagt
    19921 cagaaaatat tttcgttttc gtatacagct caagtatttt gcctggtatg gttccttggg
    19981 tagtgatttt gatcagcgag attcgcttca aaaagaagca cgcagataaa atgcatgatc
    20041 atccttttaa aatgccacta tctccttata cgaattatgt gacacttgct tttttagcta
    20101 tcgtattgat tttcatgttt atcaatcctg aaacgagaat ctcattgatt attggactcg
    20161 ttttcttgct ttacatgacg atccattact ttgtacgcga aaaaagaacc ggaaaataaa
    20221 aaaagaacga agcttcacct tagtggaagc ttcgttcttt tttattgctt ttatcaattc
    20281 aattttagcg tctcaccggg agtcaggaaa taattctcca tgctcatccc attcagcgac
    20341 aagatcgttt ctactggtac gccagttcgt tcggctattt gatttggacc ttctccttcc
    20401 aaaactacgg ttgtcgcagc ctgtgaagtc gcgctgttgg tattagtttc agaggaatct
    20461 gttgtgtctt gtgttgttcg agcttgcatt tcagaagctt ctaccgactc tgctgtctca
    20521 gaatcagaag atgttgtgtc ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc
    20581 tgcaaataaa tatcggaaat cttagcgaca tgattcgtat aattagccga taattgattg
    20641 atcaaagtat ctaagtctac accgtcttta ttttctgctg cctgatacgc tgcacgtaat
    20701 tttctgatcc cttcgtcgga gattgcttga agctcacgtg ctttttggtt agcaactgct
    20761 gataaaccag ctattccctc ttgatttccc tgaatttctg tctggtattc ttcaatcaat
    20821 gcaggcgtct tttcttcgat ctttttcgaa taatcattta cgattgcttg aagttgctct
    20881 ggcaaaccat ttgctgtttt ttcttcagaa gtttgtgtct cttttgtttt agttgatgac
    20941 tcacttgttt gtttcgtctc tgttttcttt gtcgttgttt ccgttatggt cgtacttttc
    21001 gcttctgacg aattttttcc atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa
    21061 aacacggcaa ctgctgcccc aatcatgatc atccgcttac gagctgcctg tttttgctgt
    21121 cttcttcggg tcatccgaac attttctccc cactcatttt catcatacat ttttttcacc
    21181 accaattttt atgtataata gcgttatcaa acgcttcata ttcttattgt agcaaataat
    21241 tgtcgctgtg cacattttta tctttgttat ttataagaaa atttcaccag ttgagatgat
    21301 tccaagattg tccgtcgatt tctccttgca gctgcattgt tcgttcgtct atctcagcca
    21361 tcagtttctt tatttcatat aagagcgcac ggtcttgata ttctgatttt tcgcttaatt
    21421 catctaattg ttggatcagc caatcatttg tcataatgaa gttcctttca tttcttgcag
    21481 tgtaaaggtc aagtcttcga tctgctcttt tgcctcagtc agtgcttcta cgatatatgc
    21541 ttgtttttca gattcttctt cctgcatttt ctgattttcc tgtactcttt gaataaacca
    21601 gtcaatccgc tgagtcagcg atggtttgcc ttccaatgag agatactgct gataagcaat
    21661 tagcttctcc caacgttttt cctgggataa atacataaat tgtgctaaaa cgaatggcgg
    21721 aagataggtc atatggaatt tatttgcgat tcgctgatac ggcctcaaaa attcggaaat
    21781 cgtatattct tcttttcctc cagcttggta ttctttttcc gccacacctg ttgtcaatac
    21841 gattcctagt tctttccctt ttaatacatg tgctgctcgt tccaagacat tatcttgcca
    21901 aatttttaaa tgagcaggag aactatacca gtaaaacgga aactggaaaa tgatccgatc
    21961 atgctgggcc aagagctgta gttcagtctc tatatctatt tttccgtctg gataacttga
    22021 ttccaaatga tgccaagtag ctggtaaacc agcagaagct tctttgaaaa attgctgatt
    22081 taaagattct tcgatcgttg gatggctaat gatgataagt gtacgcatcc cttttcctcc
    22141 tttttagtct tcgctttaca gtgtagcata accgccacaa gtttcttttg ccgatgcttg
    22201 tcattttaac cagactaatc aggttgtcca agaaactgcg gtttgttttt ccaactgttt
    22261 tttcaaggta tccaaagcaa tattcttaaa attttcgttt ttcatacgtt tacgatagtt
    22321 cgtagccgtt tctccggtac tctttttgaa cattcggcta aatacgacta gactattgaa
    22381 ccctacttga taggcaatac tttccacgga ttcatccgtc agagaaagaa gaaacgcccc
    22441 tcgattgatc cgtaccccga tcaaatattc cttgatactg gtacccatat gatttttgaa
    22501 tactcgcgac aaatggctcc gattgaccgc taaatgctgg gcgatttcct caacagacaa
    22561 tttttcagca taatgtgcct cgatgtattt gactgtttca acagctaaag aggaatgaac
    22621 cttttgtaca ccaccgctga cctttccgcc atcttttaaa aggactgata aaaaacggta
    22681 ggttaatgct gttaactgta attcattttc caaggtttct tccgtatctt tcatgcattc
    22741 taaaataatg tcgatatatt tttgtatgtc agaagagacc atcgtgtatt ggtcttctct
    22801 gaaaagcgac tgccgaatga tttcttctgc agcactccca ccaaatgaaa gccagcaata
    22861 catccacgga tcttctccat ctgataagta aaacgtcgag tctcctggac gaatcaagaa
    22921 cagatcacct tttttcaatt ggaattgttg atttttcaca tgataagatc ctttgccttc
    22981 catcacgaca tgcaggatgt aatcattgcg aatagcagga ccaaaagaat gaaacggcat
    23041 cgttttagag aaaccacaaa aatcaaaata caattctttt tctacttgtt gatgttcctt
    23101 ttttaaaaaa atcgattgct ccacgtctat ccctccttac tcctttagta taatgcaaca
    23161 aaaagttaaa taaaagtcac agaaatatat taaaaaggaa gcgatttctt gttaagttta
    23221 agtcattaaa agatctttgt tattcgttgt aaccggttta cttagtatta tatattgaac
    23281 aggagaggaa tacagattat gaaaagcaaa atgatccaac gtgtgtccta cgctttaggc
    23341 gcttttggac acgacacatt ctatgttaca ttaagcactt attttatggt attcgtgaca
    23401 agccagatgt tcgcaggaac agataaggca acgaatgcaa aaatgattgc tacagttaca
    23461 agcttagttg taggtatccg cttagttgaa attatttttg atcctttgat tggtggcatc
    23521 atcgataata ctcgtacaaa atacggtaaa ttcaaaccat ggttagttat cggcggcgtt
    23581 gttagttccg tactcttggt agcaatcttt accaatttct ttggcttagc tactcataat
    23641 acgactttat ttatcatatt atttgcaatc gttttcatct tattggatag tttttattca
    23701 tttaaggata tcgctttttg gtcaatgatt ccagctttga gtacggaaac aaaggaacgt
    23761 gaaaaatttg ctacgtttgc tcgtttcggc tcttctctag gtgcaaatgg tactactcta
    23821 gtcgttgtcc ctatcgttac tttctttacc tatcttgtta ctcataaaca tattgaaggc
    23881 gcttcaggct ggttctggtt cggtgtgatt gctgctgtta tttcaggcgg tactgcgatt
    23941 atcactgcct tgggcacaaa agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct
    24001 gggatcaaag atgtgttcaa agcagttgct caaaatgatc agttgatgtg gcttgccctt
    24061 gcttatatcg cttatgcgat tgccaatgta gcaacaactg ccgtattatt ctacttcttc
    24121 aaatatgtac ttggccaacc aactgaattt tggatcgttg gtctagttgc tgccttactt
    24181 ggtttagtag ctgttccact attcccaatg cttacacgcg tcatctctcg tcgctacgtt
    24241 taccttggag ggatcgcaat gatgatttct tcttactttt tctttacgat cgcaggttca
    24301 agtttgccaa tcgttatcat cgggctagta ttgttctact tccctcaaca attgattttc
    24361 ttatccgctt tgatgacgat cacagactct gttgaatatg gtcaatggaa aaatggtatc
    24421 cgtaacgaag ctgttactct atcaattcgc cctttactag ataaaatcgc tggtgctttg
    24481 tctaacggga tcgtagggtt tgtcgctatc gcttctggga tgactggtaa tgctactgct
    24541 gctgatatca ctgctcatgg cgtaacgact ttcaaagctt atgcattcta tgctcctgct
    24601 gctttgatga ttttctccgc tattattttt gcttggaaga ttacattgac agagaaaaaa
    24661 catgcacaaa tcgttgagga attggaaaac aaattggctc cagctgatac aatggaagat
    24721 gaacttcacg atgcagtagt tccttcccat taagaaagga tcttgaaaat gaagattaca
    24781 gaaaaagatt ttggccaagg ttatcacttg ataaccttgg agaacaaaaa caagctagca
    24841 ctttctatct ctgatttagg tgctagaatc gtcagtttaa aatcaaatga tcgagaatta
    24901 gttttgggat ttgacaccgc tgaagaatat atcgaaaaag atccgtatat cggtgcttct
    24961 atcggtcgca ccgcagggag aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac
    25021 caattagcca cagatccaaa aactggacat aatctgcatg gtggtgctcc tggatttgaa
    25081 ttaaaaaaat ggtcttatgt cattttgaat ggcgaaaatg aagcttctgt catttttact
    25141 actacaagtc ccgacggaga acatggtttt ccaggtacaa tggatgtgga aatccgctat
    25201 actttgacaa aagataatat ctggcgagtg acctctcgcg gcaccagtga ccaagatact
    25261 ttattcaatc cgacaaatca tgtttacttc aacttgacgg gagatgcttc tcagtcaatc
    25321 gatcaacatg agctttggct caatagcgaa gcttatgctc ctttgcgaac agatagtatt
    25381 cctatagggg taaaagaaaa tgctgcagga agtgctttcg attttcaaat acctaaaaaa
    25441 ttagcgtccg tttttgctag tgatttggat caaaaaaatc ttgttgacgg aatcgatcat
    25501 ccatttttct taaaagaaac cggcttgggt aaagaagccg ctcgcttgac tagccccgat
    25561 aaaagaatcc aagtaggtat tgctacagat gcttctagtg tggttatttt cactgcaaat
    25621 ttcggcacag aaacaccaga aatgcgcaac cgaaaactgg cacatcacgg tggcatcaca
    25681 tttgagactc aaacagctcc aggtgcagag cgcttctcag catttggttc gattcattta
    25741 aaagctggat cagtatttga aacagtgaca gaattcaaaa tcaaaacaag aaaggaataa
    25801 ctcatcatgg aaatcaaaag aagcttaaac acaaaatttg ccgaaacatt cggacctaaa
    25861 aaaactacac aatacttctc accaggacgc atcaatttga ttggtgaaca cacagattac
    25921 aatggaggcc atgtcttccc cgcttccatc acttatggaa cgcaaggtgt agcagcacct
    25981 cgagacgata ataaaatatt agtttattcc acaaactttg acgatatcgg tgtcattagt
    26041 tttactttag atgagctaga atatgataaa aaagcaaact gggcaaatta cgtaaaagga
    26101 atggtcctta aattaaaaga agctggacac tcaatcgatc atgggtttga gttgttagtg
    26161 gaaggaacaa tccctaatgg tgcaggactt tccagtagcg cttcattaga attattagta
    26221 ggtgttgtat tagaagatct gtttgattta aaaacagatc gcttggaact agtccaaaca
    26281 ggtaaaaaag tagaaaatga attcatcggt gtcaattcag gtatcatgga tcagtttgcc
    26341 atcggcttcg gtgagctaga tcatgcaatc ttattagata caaatacgct gaaatacgaa
    26401 atggttccag tgaaactaga tggttatgca atcgtcatca tgaatacgaa taagcggcgg
    26461 gaattggcag attcaaaata caatgaacgc cgtagcgaat gcgaagaagc cttggcacgt
    26521 ttgcaaaaaa ctttgcctat ctcttcttta ggagacttag atgaagaaga atttgaatca
    26581 actattgacc aaatcggcga tgatacattg atacgcagag ctcgccatgc cgtttctgaa
    26641 aatcagcgga ctttgaaggc aaaagctgaa ttagaagctg ggaacttaga agcatttggt
    26701 caactgttaa atgattctca ccactctctt cgctatgatt atgaagtaac tgggatcgaa
    26761 ttagatacac tagtagatgc tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca
    26821 ggagctggat ttggtggctg tgcgattgct cttgtcaaag aagaaaatat acctgccttc
    26881 aaaaacaatg tctatgatga atacatgaaa gttgtcggct atgctccgga attttatgtt
    26941 gcacacattg gaaatgggac aacaaagatc gatccagaag ctgttcatgt ttgataaaaa
    27001 aatcaacgat ctcagctaaa aacaaataga taaaaataac ctctcagcaa tttccaaatg
    27061 tgagcctttt gctcgtattt gaaaattgct gttttttatt gacacggaaa gagaacgtgt
    27121 gttctaattg tgttgaagag gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt
    27181 atgaaaaaga accttcaaga gatattttat gtattgactg caaatctttt tacgcctccg
    27241 tagagtgcgt tgaaaaggat cttgatcccc ttacaactaa attagtagtc atgtcctatc
    27301 cgtccgattc gacagaaaca cggggtagcg gattaatctt ggcttccagt cctgcagcaa
    27361 aaaaagctta cggcatcacc aatatcagcc gtgccagaga tcttcccttc ccctatccaa
    27421 gtgatttata tattgttgcc ccaaggatgg cctattatat ggaaaagaac agagaaatca
    27481 attctatcta taaaaaatat gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa
    27541 gcttccttga tgtaacaaat agcatggcac tctttggtaa aacaaatgcg tacgacatgg
    27601 ctaaactgat ccataaagat gtgtttgagc agaccggaat ctacacgact ataggaattg
    27661 gcgataaccc gctacttgct aaactagcct tagacaatgg ctcaaaagac ggaaagcaaa
    27721 tgtttgctga atggcgttat gaagatgtac cagaaaagtt atggaaaatc tcagatatca
    27781 ctgacttttg gggaatcggc aaacgaacag caaaacgtct gaaacaaatg ggtatacatt
    27841 ctgtttatga tttagctcac gcggattatt atcacttgaa aagcaagcta ggggtcatcg
    27901 gtactcagct ttatgcccat gcttggggca tcgatcgaag ttttttggga caagaatata
    27961 caccaaaatc aaaaagtatc ggaaatagtc aaatcctaga tagagattat acaagacgag
    28021 aagaaatcga gatcgttatc aaagaaatgg ccgaccaagt cggcactcgc ttgagacgat
    28081 ctgcagcaaa aacacaagta gtcagcttat gggtaggata ctctttaggc tatcttgatc
    28141 caaatggcaa gaccgggttt caccagcaaa tgaaaatcga agcgacaaat gctagcagag
    28201 atctagcaga tgctttattg accattttcc accagcatta ccactaccag gatattcggc
    28261 gtgtaggcgt gaactgttcc gacctcgtat acgcgacagg tttacagctt aacctttttg
    28321 aagatcctga aaagcagata aatgaagcga aagttgattt tgttgtcgat acgattagaa
    28381 aaaaatacgg ctttcaatca atcatccatg cgcatagtct cttgcaagga ggacgcgcca
    28441 tcgcacgtag ttctctcgtt ggcgggcatg caggtggtat gagtggatta gaaggtggaa
    28501 aagatgagac ggacagcaaa aaaattcatt gactataacg agtatcgaga tcggccattt
    28561 ggattaaaat gggggactgc ttttgcgatg gaagaactag tcaacggtat caaaaaaaat
    28621 gaagaagaag cacgcaaaca gcctcttcct aaacaacaaa tgacattgca agaaatcgat
    28681 agtatactga cagaagcctt tttatctcgt aaagcagtaa tcattcagct caatttaaaa
    28741 gatgaatttg gaagactctt ggatgaggtc gaagggcact tcattggtga agcctatgaa
    28801 gattacttta tgattgacca tcagccgata ttgtgggaag acgtacggta tattgagtta
    28861 aaggaaacaa aaaaatggtt tgtgacatga gaggttgtga cagaagtgat caacttcaaa
    28921 aaataagaaa ggatttttcg aaaattgctt cttaaatttt tgtggaattt tggcttattt
    28981 ttcgaagaag caacttctgg aacaacgtat attagatatc agaggttgtg aaaaagtcgc
    29041 ttagttttga gtaacaagcc aaggcgctga tttcacaacc tctgtctatc tatccatcta
    29101 ttcggtaaga ttcatgcatg acgtcttctt tatttagtat gtagagccct ttttcttctg
    29161 accattcaac ataaatcagc tgttttacat ctgcatagcc ttctttcttc aagttctcca
    29221 taagccatgc ttcagtcaat tgattggact gaagcacttt atgctgaatt tgcccttcat
    29281 ctacaagcag gatcgataaa gctttgtctt catctgattt tttcaataca ctcatttgac
    29341 cagcattctc taggataaca tgtttggcat tttcaagaga aaagcattct tgctgtctta
    29401 agattgctcg caactgctcc atctcgatat ggttatttgt caactctgtc atgttgatga
    29461 cgccgtcttt gattagaaca gatggctcgc ctttgaccca acgattgacc ttttcatttt
    29521 tttggacgat acgttcgata ccataaatca ttactgccca aagcgcaatg gcaaatagga
    29581 catgccctac atggacattg tcatcatagg ttgattcttc taagatccca cctaaaacaa
    29641 gcgtatagac taaatcaaac ggtgtgattt ctgacatgga tttcttccca agcaaacgta
    29701 ccactaataa taaaccgatc aacccgaaca ccagtttcat tgtaattgaa aaaatcattt
    29761 tttctccccc taactatctg ttaattaaat tatatatgct ttctcacgaa cacgcatgga
    29821 atatacaagg aagaaatgta tccttttact aaaaaaagga ctgtgacact gtagtgaccc
    29881 ccaaaagtta gacttttttg gagtggagaa atccactcct tttttttatg ctctaaaatg
    29941 atatttttac acaaccgtta cggttgtttt acgaaattgg acg
//
LOCUS       DACDRK010000028        29851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDRK010000028 DACDRK010000000
VERSION     DACDRK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.028"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(148..1092)
                     /locus_tag="IWJ77_001354"
     CDS             complement(148..1092)
                     /locus_tag="IWJ77_001354"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9452983.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1388..2332)
                     /locus_tag="IWJ77_001355"
     CDS             complement(1388..2332)
                     /locus_tag="IWJ77_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9452984.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2553..3767
                     /locus_tag="IWJ77_001356"
     CDS             2553..3767
                     /locus_tag="IWJ77_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9452985.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3789..5804
                     /locus_tag="IWJ77_001357"
     CDS             3789..5804
                     /locus_tag="IWJ77_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9452986.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5887..7260)
                     /locus_tag="IWJ77_001358"
     CDS             complement(5887..7260)
                     /locus_tag="IWJ77_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9452987.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7286..7570)
                     /locus_tag="IWJ77_001359"
     CDS             complement(7286..7570)
                     /locus_tag="IWJ77_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9452988.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7572..9587)
                     /locus_tag="IWJ77_001360"
     CDS             complement(7572..9587)
                     /locus_tag="IWJ77_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9452989.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9766..10071)
                     /locus_tag="IWJ77_001361"
     CDS             complement(9766..10071)
                     /locus_tag="IWJ77_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9452990.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10160..11191)
                     /locus_tag="IWJ77_001362"
     CDS             complement(10160..11191)
                     /locus_tag="IWJ77_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9452991.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11398..12273
                     /locus_tag="IWJ77_001363"
     CDS             11398..12273
                     /locus_tag="IWJ77_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9452992.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12425..13267)
                     /locus_tag="IWJ77_001364"
     CDS             complement(12425..13267)
                     /locus_tag="IWJ77_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9452993.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13359..15236)
                     /locus_tag="IWJ77_001365"
     CDS             complement(13359..15236)
                     /locus_tag="IWJ77_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9452994.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15233..16654)
                     /locus_tag="IWJ77_001366"
     CDS             complement(15233..16654)
                     /locus_tag="IWJ77_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9452995.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16657..20046)
                     /locus_tag="IWJ77_001367"
     CDS             complement(16657..20046)
                     /locus_tag="IWJ77_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9452996.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20303..21694
                     /locus_tag="IWJ77_001368"
     CDS             20303..21694
                     /locus_tag="IWJ77_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9452997.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21767..22414)
                     /locus_tag="IWJ77_001369"
     CDS             complement(21767..22414)
                     /locus_tag="IWJ77_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9452998.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22422..23108)
                     /locus_tag="IWJ77_001370"
     CDS             complement(22422..23108)
                     /locus_tag="IWJ77_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9452999.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23114..24187)
                     /locus_tag="IWJ77_001371"
     CDS             complement(23114..24187)
                     /locus_tag="IWJ77_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9453000.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24409..25083
                     /locus_tag="IWJ77_001372"
     CDS             24409..25083
                     /locus_tag="IWJ77_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9453001.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25088..26428
                     /locus_tag="IWJ77_001373"
     CDS             25088..26428
                     /locus_tag="IWJ77_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9453002.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26577..27563)
                     /locus_tag="IWJ77_001374"
     CDS             complement(26577..27563)
                     /locus_tag="IWJ77_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9453003.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28287..29318
                     /locus_tag="IWJ77_001375"
     CDS             28287..29318
                     /locus_tag="IWJ77_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9453004.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29427..>29851
                     /locus_tag="IWJ77_001376"
     CDS             29427..>29851
                     /locus_tag="IWJ77_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9453005.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         8838 a         5600 c         5373 g        10040 t
ORIGIN      
        1 tgttcatatg tacggataca ttttcggacc gattaatttt aaccattctg tttatttgct
       61 tacctattag atatcaataa aaaactgctt aacagctatt tcagctgtta agcagtttta
      121 ggatttttag ttcatattca ttgtttctta taatacagtg ttcaatactt ctcttaaagc
      181 acttgttgat ttgctgaaca ttgctttttc ttcttcgttg attgataatt caacaacttc
      241 acgaacacca ttttcgtcaa cgattgatgg cacacctgta aagatatctt tttcgtcgta
      301 ttcaccagtt aaataagcag atacaggcaa tacagcttgt tcgttattca agatagcttt
      361 tacgatacgt gcagtactca taccaatacc atagtaagtt gcttttttac gatcaatgat
      421 ctcataagca gcattacgga ctttatcagc aatcacatct aattcatctt gggcaatacg
      481 atggtctttt tctacaattt caaacactgg tttaccaccg acagttgtat gtgaccatgc
      541 agcaacttct gaatcaccat gttcacctaa gatatagccg tggacgctgc gtgggtcaac
      601 ttttaatttc aatgcgattt ctttacggaa acgagttgta tccaatgttg ttccagtacc
      661 gataacacgt gatgttggca gaccagattc gttccaagcg atatatgtca aaatatctac
      721 tgggtttgaa gcaacaacga taataccgtc gaatcctgat cccatgattt cttttacgat
      781 ttggcgcatg attgacgcat ttgttttaac taaatctaaa cgagattgac caggtttttg
      841 gttgatacca gctgtgataa caacgatatc agcatcttta cattcttcat agccacctga
      901 ccatacagcc acattttcat cgccccaagc cataccatct aaaaggtcca atgcttcccc
      961 ttctgctttt tcttgattca catcgatcaa tactaattca ttagagattc cttggttgat
     1021 cattgcataa gcgatacttg ttccaacaaa acctgtaccg acgattacta ctttacgact
     1081 tgtttttttc atgaataaac agctcctttt cataactatt tgttactttc atgaaaagta
     1141 tagtcttgtt tattcatttt atcaacatgt taattataat agttgttatt ataatttttg
     1201 atggaaacga attcctgaat acgcataaca gataaatgac aaatgtttac acctatatcc
     1261 cctgataata caaccaaaag tttagaactg aaaataaatc caaaaaaatt cgctgcaacg
     1321 atataataag acagcaatcg cacctaaaac aattttcctt catatgcttt agtgaaagat
     1381 tttcttctta atgtttattt ctccattcat tgggagtaca tgttgaatac ttcttgaata
     1441 atttgaataa agaagtcgtt tgtttatatc cgatacgatt agcgatttct tccaacgaaa
     1501 aactcgtttc ttgaatcagt tccaacgcaa agtcgaattt tttctttaga accagttctt
     1561 gaaaggaagc atcaaaatac attttcaatt tattactaag atagaccgga ttgtagccaa
     1621 aatgagctgc tgtttctttt aacgtacaag aagtaaaatg cttatcgata taatctaata
     1681 ctctaagcat ctcattttct gcgcatccat ctgctgatga aaagctttgt tgttctaata
     1741 aatgagtcaa ttcgatcagt aatagtgatg tatagagatt taacgaacgg atcttatgac
     1801 tgtatgtatc aaaataaatc aacagcatat tttcaatcaa atgcgtcaaa cgacttgttt
     1861 ttccgccgcg aaaatgaatg aattgtgtat gttccccttt ttcttttgat gcatttatca
     1921 aaaaagaaga aagtagatta tttgtgcttg ccaaatgatt caatatctct gtagataagg
     1981 tttcttcttt taataagatg ttgattaaaa tatcctcttg ccctgtgtat tcgattcttt
     2041 gagtagcttc tttatcaatg atcaccacgt cactttcttc catctctatc ctttctccat
     2101 taataatttg taaacatgta ccgctataca tataattgat ttcaacaaaa tcatgtgtat
     2161 gctcgggaac ttccgcatat cgacggtgtt tactgatatg aatatttccc tgttgaaata
     2221 gatcccctcg gtggattttt ggcggtgtct gaagatggta agctaagttt aaatcattga
     2281 tattcattcc ttgttcacgc tgcagagttt cgattgattc ttcgttacgc aaaaaaaatg
     2341 tctaattcag ctagttccaa ttgactccct cctttttgta agcggtttct ttcgcttatt
     2401 agttcacgct gtactgctat tatatgcaaa gctatttttg gtttcataaa gactttctat
     2461 ggttgggaca tgaacttcct gtgattccgc tattgagaga ataaccactt catactatat
     2521 ttttgataag cgcttacaaa aatgaaagga acatgcttat gaataaaaag atgatgaaat
     2581 tttctctgct gtcagtctct cttattttga cttctgctgg tgcagtcacg tctgcaattc
     2641 ccagtatgtc agctacttat gccacgcgtt ctttatctgc tgttgaatta ttaacgactg
     2701 ctccctcttt tatggtcact atttttgttt tgattagtag ttttattgct aggaaaatcg
     2761 gacaaaaaca aactgtgatc atcggattag tgattgcatc gatttgtggt atcctccccc
     2821 tcttctcaca aagttacagt attgtcttag cttcaagagc tggactaggt attggctttg
     2881 gattgatcaa ttctttggca gtcagcatga ttgctgcctt ttatcacgga gatgaacgag
     2941 cctctatgat tggttttcaa agtgcatttc aaggatttgg tgcagcttta atgacatttg
     3001 ttgcaggaca gctagtacag ttcggttggc aatatacctt tttagtctac gccatcagct
     3061 tacctattct tgtcttgttt gttttgtttg ttcctacacc tgaaacaact gaacttcaat
     3121 cgcatggcac agccccaaca agacaagtaa tcaaaacgaa tcctaaaatg attagttatg
     3181 gtatttttct cttgctggtc atcgtggtat ataatgctgt atcgattaaa ttagccacag
     3241 tcctgacgac ttattctcta ggaaacgaaa ctaatgcagc gaatatctta acaatcatgc
     3301 aattggcaag tatggctgcc ggagtttgtt ttggctggct tcagacaaaa gcacatcgct
     3361 atatgcttac actttcttta ctactgatgg gaatcggctt tgtattgatt gccactgtga
     3421 taaacctgta tgtgattggg tttggtgcga tcctaacagg aatttctttt tcgttattca
     3481 ttccttactt attcaaccaa gtaagtatcc aagcgcccaa gtacgcggag agtttcaata
     3541 cttcaatctt attggttggc gctaatttag gtactttcat tgcaccatat ggtttgctct
     3601 ttctctcttt tgcttctctt gggagtaaaa cacttgctgt tttcgtcaat ggcagtatct
     3661 tgctgttgat tagcgcgctt attagccttc tagtagtaca caggacgaat agaaagacag
     3721 tgataagagt agatgaagaa ataaaagaac aattgacaac aaaataaatt tgatggaggt
     3781 cgtgtaaaat gacaaatgaa aaacataaag atagatggct ttggtatcct ggagactttg
     3841 agatccgcca tggactgcta caaaatttcc aacgggaaga acgaggtttt gactggccag
     3901 cgtactggta catggatgat tgccatcgga acgtcaagtt taagagatat tactttcttg
     3961 atcaaccttc catgtttaaa gtgacgatcc aaggagttgg gtatgtagaa attaatggac
     4021 aaaaacatcc atgtggaaaa tggctgactt gtccagctgg taaagcaaaa attcgtatct
     4081 ttgtaggaca tacatcaggg ctaccagcga tgtttatcga gggagacgaa gtaaaatcag
     4141 atattggctg gacagcaagt aattttattg aagaatatcc agctggctgg agtccgcttt
     4201 atgtcgatat cgcaaaagat ccaaatcaaa tctattatca aaaagaatgc gtccatccag
     4261 tcactgaaac ggagattggc ggaggaatct tattcgattt tgggcgagca gtgaatggct
     4321 gtgtagaact aatatctctg gatgaccata tcgaatctat cactctatgt tatggagagt
     4381 ccgatgtaga agctatggat atcgattatt gctactataa gcaaatagga atgacaagaa
     4441 aaatgaaagc tcgtaaacgc gcttttcgct atttgtttat accagaggtt tctactggaa
     4501 tggttcaact tcaagttttt catgaatttt tatctctttc gaaacaagct gctttttcaa
     4561 gcgattcttc tttactgaat cagatctggt cagtatcaga agaagccttc aagttatgta
     4621 gtgatttgtt ttttatcgac ggtatcaaac gagatcgctg gatttggtct ggagatgcgt
     4681 atcaatcatt catgatcaat caatatcttt tttttgacga ggaaatcaat aaacgaacga
     4741 tattagcatt aagaggacaa aatgaaatca agcagcatat gaatactatc gttgactatt
     4801 cgatcttgtg gttgattggt atcgaaaacc actatatgat gacgaaagac tatgaatttt
     4861 tacgcatcgt ctaccctaag atgcaatcat taatgagata cttgatggag caaacgaatg
     4921 aattagggtt tatttatggg agagcaaaag attggatatt tattgattgg tctgaaatgg
     4981 ataaagaagg aacgcttgct gcagaacaag tcttattact gaaagcctat cattccatga
     5041 tcacttgcgg agatatctta gctgaaacat atggagagaa cgaaaattat catactcatc
     5101 tctatcaaga aaaatatcaa ctattgaaac aaaacctatt tgactacttt tgggatgaag
     5161 aacttggcgc ttttatcgat tgctatgaat ctgggagaag aaatgtgacg cgtcatgcga
     5221 atattttcgc tgttttattc gattttgcta acaaagacca acagcaatcg atcttaaaga
     5281 atgtcttatt gaatgacact atcacacaga ttactactcc atatttcaaa ttctatgaac
     5341 aagatgcttt atgcaaactc ggacagacga atatcgtcta ccaaacgatt cttgattatt
     5401 ggggagggat gttatctcat gatgcggtaa ctttctggga agaatacgac cctactcaaa
     5461 caggaaatga aaagtacagt atgtatggag atccttatgg aaaaagctta tgccatgcat
     5521 ggggagcaag cccaatctat cttttaggac gctattttat cggtttgtat ccaacaaagc
     5581 caggttatga agaatttatg atacaaccta atctagattc atttgaaaca ctgaattgta
     5641 cgctacctat caaagatgga aagatttcgc ttgaaaaaac aaaagataag atcgtgatta
     5701 cttctgatag aacaggcgga acagtgaaaa tgaatggtaa aagttatcct cttctagcaa
     5761 aacagccact tgtcttaaca agctattcaa aaataaatgc ataaaacaaa aaactgcttg
     5821 cgacatatca gaagacttta aatcttcttc tgatattgtc caagcaattt ttattttgaa
     5881 gtttgcttaa ttctgttctt tagaaacaat gtttgttctt tttccggcaa aaagaaagct
     5941 agataatacg acaagtaaaa agaaagcgat gattccaact gtcaaggcta tttgaccgta
     6001 ttctcctagt ttcccaagta caagtcctgg tactagataa tcagtatcgg caaatgtaga
     6061 agaagcgatt ttaagatctc ctaatactgg aaggataaat aatggcagcc aactaatcaa
     6121 taagctattg aaagcagaac ctaataccgc ccctcttcta ccgccagtcg agttgccgaa
     6181 gacaccagaa gcagctccgc agaagaaatg tcctactacc ccaggaatga taactgtagt
     6241 ttgcaagcct atcatagcaa acatggatag tgttcctaca aagaagctaa caaaaaagcc
     6301 aattaataca gcattaggtg catatggaaa aacgatagga acatctaatg ctggtttaga
     6361 atcgggtacg agttttttag caatcccttg gaatgctgga acgatttctg ccaacaccat
     6421 tcgaacccct tgaaggacga taacaaaccc agctgtgaat gttccagctt ggatcagtgc
     6481 aaagattaca gcgttgtctc cattgcttag ttctctttca acaaatccag gaccagctaa
     6541 caaagcaagt gcgacatata cgatcgacat caagagcatg atcgatacag tagaatctcg
     6601 caaaaagctg aaacttttag gaatctttat gtcctctgta gaacggtctt tgtttccaaa
     6661 aaactttccg atccatccac tagaagcata accgatattt ccggtatgcc ctaacgcaac
     6721 ttgattgttt ccagtgatgt tcttcataaa cggttgacaa agagcaggcg taatagttaa
     6781 tagtgttcct tcgaagaccc cgcctaataa gatcatccac aaatttgcag taaaccccgc
     6841 gcttactaaa atgactgctg ttagacaaga aacataaagc attgcttgac ctgtaagaaa
     6901 aatgaattta tatcttgtga atctagcaag taaaatattc acgatcatcc ctactagcat
     6961 gatcaaagca gtaggtgtac cgtattgttg caaagcaatc gccactactg cttcattgct
     7021 aggaactact ccttgagtat gcagagctgt ttggaacatc gaagcaaaag gggttagtga
     7081 attactgatt aaaccagcac cgccgatcag tactaggaat ccgacaaatg ttttgatagt
     7141 tccttggatc acttctgaaa atttcttctt ctgaagaacg agaccaagca gtgcaatcaa
     7201 agaaacgagg atggagggct gactgacgat tgacacaaac aaattcaaca atttatccat
     7261 tgatagttcc tcccttgaat catttttatt cttttgacaa gatcttcgtt actttttctt
     7321 ttacttcttc gctgtcaatg atcgattgca aaggtacgag cttttcatca ggtatagaag
     7381 ttagcgtatc tttcaaagaa tgctcaatga agaaataatc agccatatcg ctagatacac
     7441 ttccaacatc cgtatgactt acttcaaact cgtccacatc tgctccaaga tctgcaagtg
     7501 cttgtttgat gttcatctct accataaaac ttgttcctaa tccactttga catactgcaa
     7561 aaattttcat atcattcgtc tccttttctt atgatcgcta atatttcttc ttttgttgat
     7621 gcgtgtaata aatcttgaag attttgttta ttagaaagta gctttgttaa atttgccaaa
     7681 gcccgaagat gtgcctcatt atcgatagca gctaaacaga tgaacaaact aatgggatgg
     7741 gctggattat caagcagatc cacaggttgt tgaagtttca gcaaagacat ccctacttcg
     7801 tttacgccat cttctggacg agcatgaggt aatgcgatgt gatcgccaat atggataaac
     7861 gcaccatact gctccacgcg ctcgatcata gctttgatat aactttcttc tattttattt
     7921 tctgataata gcggctgtgc ggcaaatgtg attgcttctt gccagttttt tggttgatcc
     7981 gataattgga tcatttcttc tgttaataat tcagatagca taggacgttg atcctccttc
     8041 ttttctaaaa gtttgttcat tttacgattt aatacttgat agagcttttc ttcgtctacg
     8101 ccttctttta attcgatata aggcagtagc gccgacaaca attcttgggc agaaggcatt
     8161 gaaaatcctg gtatcagcca atctctttgt acttgattga tcagctgatt tttttccaag
     8221 tcagtcatga ttggatgcag aacatagaga gactttttct tatcagtaag tgtgagaggt
     8281 acagtagaaa aaataatatc gtaagaagtt tcgtcaacct cgtgtaagcg gtcagctgaa
     8341 ttagtctctg taaaaaagat catcgggaat aattgctgaa gttcactttt taagatcaat
     8401 gaagagctaa tgccacttgg acagacgatc acggctctta tttttctatt tctttcttct
     8461 gcgtccgctt ttctgatctc tccgccaaat agtatagtga aaaaaccaat ctcttcttca
     8521 gggatgctct tccctattcg tctttctaac ggaagtaatg cttttcttgt catttcgtag
     8581 atggaagcat attgatgctt aatttgatcg atcatcacat taggtaaata aaaagaatag
     8641 gaaatacgaa aataggcagg gaccaagtga taaaagacat tcatcaataa ttcacgaaac
     8701 gaagcaaatt ctacggccat caggaattcc atgcgatgga tgacttctga agaacattcc
     8761 agcaaaaaat caagtgcagg ctcctggaca tctccttcaa cgatcgatgc taagcaaaga
     8821 gagaaaaata taatctccga attttgatgt tcggaatagc caaggccagt caatacttgt
     8881 cgactaatgt cagtgactgc aagagaagcc aacagttcag catcttttac cagttccatg
     8941 gatgtttttc caattcgaat ggttgtcaaa ctcaaataat aagctaattc atccattcga
     9001 tttggtgcga atcgaagatc tgatagtgaa atcgttttat ctagcaaatc tctaactttg
     9061 gcgtaataat caatggaaag ggttgcggtt aaatacgtga gcccaaatct tccagcaagt
     9121 gactgcatca aacgagcagt gcgatttttt gcttctcttc ttaatattgc ttctgaccct
     9181 agcagcctaa aaccagtttt cctagtatac gacagctgac aatcttccac ttctaattca
     9241 tttcttatct ttttgatatc tgacaggact gtccctttag aaacctgaag aaaatcttga
     9301 aaatgaaaaa cagaaacaga cgttgcagtc gaataaacca tcaaataaag aagatctctt
     9361 cgctcttgtt cggtatacac agcttcaaat cgagtaatat ccaaaaattt ttctgcccag
     9421 tcgattgctc ggaatttctt tatacggata cgggtatcat ccacatattc ggcaaaagga
     9481 gttaattgtt tgatcgcctc gcttgtattt acttcgatga tctctgctgc tttttctaga
     9541 ctgatttctc ttggtgtttt caggagataa ctaattttgt agttcacagc gctgttcacc
     9601 tcctacttat attctagtat ggtagcgctt ttaaatcgag gggtgtttgg tacaacttac
     9661 agttggattt tttttacgac tgtaccgaac gatttctatc gaatggatac agaaaaaaag
     9721 caggtgacca aatgtctcct gcttttatga attgatttcg tccgatcaat gatgatcatg
     9781 tttatttttt gaggtcagtt cttcaaattt ttctttgaca tcttcaaccg ctttttcgat
     9841 atctttttca aagtgattgg gctcattagg ctttgtttcc tcttctacag aatttgaacc
     9901 agataaagct gtttctttat tttctgtttc accagagttt gtgccagcta atgattcttc
     9961 tttgtcttct gtttcaccag aattagttcc tgctaaagcc acttcttctt ctgttttagg
    10021 ggccattgaa cctcttaatc gtttgtgttt ttcaccgtca taatttgtca tgttggttcc
    10081 tcctagtagt tctatacttt ttcatcgtac aataagacag tttatgcgtc aaaaaacaaa
    10141 cccgattctt acaaatatgt catatttttc cttctgattc ttgaatgctt ttgattcctt
    10201 cttccaaaat tttcatttgt aagattgttt cttcatcttt gatgactttt tctgcgccat
    10261 taactaatgt ctcgtaaatc ccttcgtaga cacggctgta atcaccaacc tcggaaatta
    10321 ctttttcttc atgataactc ccttgttcat ctatataggt caatacacca taatgttcgg
    10381 gcaaatcgat accaaagtct tcatgatcag gcatgtaaaa cattttcaga tgttcttctt
    10441 gtcgatcttt tgtttgtttc acgaacatcc ctttttttcc gtaaacgacg aaacttggac
    10501 gttctttgat gcggaaataa ctggatttta tcgaaatttt catatttcca tagaaaaaat
    10561 ccagatcgaa gtaatcattc attcgtcctt tccctaaaag ttgacgtaca tcatagctga
    10621 tatcatctgg ttcgccgaaa taagaaagaa cttgatctac tgtatgacaa gcatgtccat
    10681 ataaatagct gttcgcagta gaaaattcat gcgtattttc tggtacttcc ggtctgaaat
    10741 aatcataatg catttctact tctaaaatat cccctaattt tccgctttcg attacttttt
    10801 ggactgtcaa gaagtcagaa tcgaaccgtc tgttttggta acattggata aatagacctt
    10861 tttcttttgc taatgcaaat aactcttctg cttcctttga cgtttctgca aaaggtttct
    10921 ctactagtac attcttacca tgttcgagtg ccatttttcc atattcataa tgaaattgac
    10981 taggtgtagt gatgacaact aattggatct caggatcatc ccaaagatca gacagctgat
    11041 cagtatattc gatgtcttcg aatgttttcc acactggttc agatggggaa aaaatttttt
    11101 tgatattgat tttgttcgta agttttcttg aaaaaggcaa atgatagcga tttgtactct
    11161 ttccattgcc aagataagcg attgtaagca tgagtggtgc ctccttttat tgatgttttc
    11221 agtatatcct agaaaagcgc agaaagtatt tataatagct attctggtaa aaaagttcag
    11281 aaaaacactt gttatcattc ggtttggtga aattaatgtt caaagtaagg ataaaaatca
    11341 aaaagatagt agcggaacca agtgtttatc agtaagatag aatttggagg tcagatcatg
    11401 cttattgaag aaaaactaac aaaacaagag ttgtttacca cgacagagaa acggattgtc
    11461 gattacatcc gtaggaatat cgaagctgca gtctacatga cgattgaaga attagcaaaa
    11521 gctacgtata cttcccattc agctatcatt cgtttatgca aaaaaatggg attcagcgga
    11581 tttaaagaat tccgtttcga gctggggcgg gaagttcatc aattgattgc tcagttcaat
    11641 caaacgattg atcctaattt tcctttttca agtgaagata gcactcagca aattgcaaag
    11701 aaaatggcgg atttatcgat ccagtcaata aaaaaagcac aattgcagat tgaacatgaa
    11761 gatttagatc aaatcggtaa agtattgaac aaggcacagc gaatctttct ttttgcaaaa
    11821 ggtgattctc aaatcacggc aaggaaattc cagaataaaa tggtcaagtt gaataagttt
    11881 ttgatcatgg ctgaagaata cagtgattct tcttggaatg cagccaattt gactagcgag
    11941 gattgcgcca tttttattag ctacagcggt cgcatccatc attatgaacg aataatgacc
    12001 tatttgaagc atgtagaggc ccctaccttg ctgattaccg gaaatcagca ttcagaaatg
    12061 gccaaacagg ctgatatgtg tcttgttata tcgcaggagg aatatgattt tgctaaagta
    12121 gcgactttct cctctcaaat tgccttcgat tacgtattaa atacgttatt ctcagtgata
    12181 tattctcaaa attttgaaga gaatatccgc aacttgaaaa acaaacaaga attgatgcaa
    12241 aaaggccttc ttaaagaata tcggaaaact taagaaaaga tcctcattta gtaataagaa
    12301 agatagtgtt tgataggctc tctttgatat catcataaaa acaggctgaa gttttctttt
    12361 tcaaacctca gcctgtttgt ttacggaacg attcgaatag atatattata gaaatgagga
    12421 atagctaact tctgtttctt attttgaacg attgacgaaa aagatatata agaaggagga
    12481 aaatcagtat acatgcagct aacaatccat acaggaaaaa tcgtttgttc tcggatactt
    12541 tctgtacttc tttttgaact tcttttgcat gataaggaac ccggtgccct ctgacaagta
    12601 agcgatgact gttgaccata taaggtgtac aagttaggag tgtcacgaaa tcctgccctt
    12661 tttcgattct cagatctttt gtatcagtcg gttctaccgt cttgatctga tcgacttgat
    12721 aagcgagtgt ccgtccattg atttcgatat agaaagaatc acctaaagtc agtttcggta
    12781 agtctgtgaa caactttgct tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat
    12841 tttctccccc cgtaggataa gacgttcctt ccaataatga ggcacctttt tctagcagca
    12901 cagtattcgt ttcatcaaaa attggcaaat ggacattgat ctcagggatc gtcaaaatac
    12961 cgatcgtatg tttctcaaaa taggttctat cctttttcgc aggttctttt ttcttagtaa
    13021 agggatctgc acctggattt ccaccttctt ttgctaattg ctggtttttc tttgtcatgt
    13081 tttcttggat tttagccatg acttcttcat tttctttgac cgcttgttgc tgataatgag
    13141 agatgatctg ctgatccaga taattattca gcgcgtcgct aacaaatgga taagctagta
    13201 tccctattcc tgagagaagc aagaaaagca tcagcagatc tagaagtttc cttttctttg
    13261 gtttcatttg tgttctcctt gtgatgactg tttgataata aaagaaaaag gagcatttgc
    13321 gtaatccgct gctccttttt ccaattcttg caaaaagact aaatctgact gtgcttgcgt
    13381 ctagcaaagt acagtccagc aatcagtaga aggactactc ctgcaccgat atacacatag
    13441 attcccttac cgcctgttga aggaagtgta cctttgtgtt tatttggtat tttttcagga
    13501 gaaatcaact gtcctgctgt tacataagat tgttcatcga tagtaaatgc tacacggttt
    13561 gttaatggaa catatttttc tggcgctttc gtttcttcca gatagtacgt gccatatttt
    13621 agacctgtca catcgattaa accgttactt gtggttgtaa aaaccgttgc tgattctttc
    13681 gccgataccc agctgacggc ttttgtggat gggtcgatcg ataaatattt cgctgtgtca
    13741 ctatcttgat cacgaacgac gaattctgct ccagcaagtg tttggtctga tgtaacgtca
    13801 ccatctactt taacaaatcg tttgccccca gtaacgacat cgactgacgg tggtgtttga
    13861 tcatttgtat gcccgttatc gacatttgct tggttagaaa atcctttggt tggatctgct
    13921 ttttcgttca aatgcatata gtaaacgaat ttcaatgtac cgccaggagt taatgaagga
    13981 atataattcg gatcaactga aaccgtgaat ccgtctgttt gctcagtgac agaataattt
    14041 actgggtcga tttctttatt tccatcatat aaggcataag catacgttcc tgaagaatca
    14101 ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt gtacttgttt
    14161 tgcgttcctt ctttatcagc gatacctaat gggatattga cagaaatctc atattgaatt
    14221 ttttcaccga ttgcgacatc ttttccattc aattgaggtg ttgttttatc aactttagac
    14281 gtatcatttt tgatggtctt ttctactggt gtttggctat ttgccaggat ctcaaaaggc
    14341 gtgattgttt gcttttcaat catttccgca ttatcaggag cttttacttc ttctaaatta
    14401 tattttccaa cttctaaatg attaacgatc aactggcctt tttcaataga tgcctcggca
    14461 aagtcattgt tgccgatgtc ataagaatga ccagtaatga aatgtttggc tttggtttga
    14521 tcagttgtcc aagtgtacaa tccatctgtg acactttgga tgtatttttt gacgcttggt
    14581 gttccttctt ctttagaaat aataaattct gctccattta gtgcttcgtt ttcggcagta
    14641 ccgatttttg taactttcaa cgttccatca ttaccgactg tatttttagg gtagagatgg
    14701 atagtatcta gttcttctgt cccgtattta taagagccat ctgcttgttt gatcatctca
    14761 tatacaggga aagctaaaac catgtttgcg gcagctgaca ctccgtcttt tggttcttct
    14821 ttgatcgtgt agactgcatc tttcccattt tgttttttag gtaaagataa agtgacattt
    14881 ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga ctggactgct
    14941 tgttttgctg catccacgga cgctccttta tctcgttgcg cataaaattc ttgagtgacg
    15001 ttataaactg aaaatgaaat atcggctaat ccttggtatt gatcgaattc gctcatttct
    15061 ttcccgctgt tttggattaa aggatcgggt aaatcagtca ttttcttttt atgaaggatg
    15121 acttgagctg ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat
    15181 ggtaaaataa ggaaaaggac tcctaacata acgtttattt ttttatgatt tttcattttt
    15241 tcactcctgt tctttttttg ataaacaacc agattgcaaa gcttgcgaat accataccaa
    15301 caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt
    15361 cttttttatt gactactttc aatatactgt ccgcctcttt atcctcagaa agaaaagttg
    15421 aaatggtaaa cggaatttct gtttcactta aaatataacc agaaggtgct tcgatctctt
    15481 ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa
    15541 tcaggtgaag cgcatctgat tcgttctttg tccacttgta tccgttggct gtttgctgga
    15601 ggtaatttcc tgctttgttt ttaataagga atactgcgcc agctaatcgt ttatctggtt
    15661 tatttgtctc gattttgaca aattgcttac tccctgtata tacagagact ttctttgtac
    15721 tgacaccaaa accaaaatcc agtgttccgt tgttgttgat ttccttgttc gcctgtgctg
    15781 tactgcttag ctgcatctga taagaaatgg tcagtttttt tccagcatat ttttccaacg
    15841 aagggatcga aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca aagccttctg
    15901 tcagcttttc tccattcgat gaaatcgtta aactattagg taaaaacgtt aatgcttcat
    15961 ccgcactatc tgacaattcg aacttttgat agtcaaggat atttacagga attgtcgtat
    16021 gcaaggaata agtgattttt tcaccaatcg taaaatcatt tctaggctct tctaatacct
    16081 tctcaaaagg tggatcaggg tagtcgtttt cttcattttt aggatacaaa tggatagttg
    16141 aaagtttttg attgttttgt ccatgtacag gaagaacaac taccatattc tctgcctttt
    16201 ctttgacgac ttctggtact ttggattcaa tgaaaagata cgctttatct cttttttctt
    16261 gatctttagc ggccagagaa aaagaagcga taccatcttc gttgttaaga gttgtagttg
    16321 ttgcttctgc ggtaaacata ccggaagacg caccgttttt tgcgatctct gcttgtgctt
    16381 cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca tcataaactt
    16441 ggaatgtgac accatttaat cctcgatacg tttgtaataa agctttttct tgtccgtcat
    16501 ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa
    16561 ccatttctgt ttctgtttct gttgctacct gagtaaaagc tggtttaaat agtaacaaga
    16621 agagacacat actaagccaa ccaagttttt tcatatttag ctgccccctc tttctttttt
    16681 caaaaaaaga taccaaatag aaaacgcaca accaatcatc cctactagat agattcctaa
    16741 acggccaatt cctcccgttt caggtaatgg tacttttgcc tgattcgtga tgtctaaaga
    16801 aatctggttg tttttggctc ctggtgacag aacagattca tgatcttgtc catccacttg
    16861 aattgaccca tcttcgtgta taactatagt aactggctct tttagacctt ggtatccttc
    16921 tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt aaattttcga atagaaaggt
    16981 atctgtttct tgtccatctt ttggcgattc taagtccatt tctggtccct gcagtctgaa
    17041 ttttgctcct tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc
    17101 ttttagtcga ttttgatgag tgagcgtcca tacattattt gtcatctcgg tattttttcc
    17161 cgtgtaaatg atttttccat atttatcgat ttggaattca taatttcccg gcgaagtatc
    17221 atatccggca ggaccagctg tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa
    17281 actggcaaga cctttttcat tcgttgtttg gctgtcaatt ggctgataag taccatttgc
    17341 ttcttttttc tgtagggaaa atgctgctcc tttaagattt atctctttgt ccgtcccttg
    17401 catcgcatac ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg
    17461 gatcgtatca tcggaagtag tgactgtttt tccatcaatg gttaccgtac catcagaagc
    17521 gatcttgatt tcccaagtag tctttttgct taatccatgc ccttctggag cttttgtttc
    17581 tgtcagcgta taggtcattt ctttttgcaa tttgacattt tctggaagag aataggttcc
    17641 gtcgccatga tctgttagta aagtatcaat agaatctcct gttaatttga aaacagcgcc
    17701 aataagatcc ttttcacctg tagaggaatt tttcgttatt ttcaagttta acggtgtgaa
    17761 ttgtttagta tttttccatg tcattgcatc tatttgttga gaatcgtatc ctggtacagg
    17821 taattctttg attgtttggt aactgaatgc ttgaccttga ttattgtatt gaggtagtga
    17881 taatatctct tgataacttt ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc
    17941 ccacgtattt gttgtatctt tagctggttt aatgattcga atatatccgt ttttccaagc
    18001 tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg gacgatcagc
    18061 tagattgttg tcaaattctt cccataactt tttgatgtga agactgactc cgggagcttt
    18121 agcagatggt ataccgaatt cagctaattc attggtatca atgtttggct ggaaagttgt
    18181 cctgccgttc atttgatacc agaaatttgg atggaagtcc tcattttctg tttggattct
    18241 cacttggtaa tggatttgga tttcttggtc ttttcctaaa tagatctgat tgctcttgat
    18301 ggtatttcct tctatggaaa tagatggagt gaccgttgta ggacttgtcc ccacactctt
    18361 gactgaaagt gtaccaggct gataaatgaa tggttctgcg attggatcat ttatttgtcc
    18421 attgcttaca gttgctgaga tctgtacagc ttttttggct aggtattcag agatatctgt
    18481 tgcatgatca gctgattcat agtaaagatc gcctttttca tctgatgaaa ccatttgtcg
    18541 catacgagac tctacttctg cttttgagag accagctgcc ggatcgcttt gaagttggat
    18601 gccaagacca tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc
    18661 gataaacgta ctgtcgattc ttctggatag attgttttgg tcaggtgcat catagattct
    18721 tgagattaga gaagtatttc ccggccgatc ttgcgtatta gaaaactgag ttccgtaata
    18781 attgctgctt gattgtgcgt gtacccgctg tactttatag gaaaatgttg gtacaccatc
    18841 cgtcagcaaa acgatcactt ttttatgtcc atttggaacg gatagcatgc ttcctgcatc
    18901 tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa tggattttac
    18961 ttgatttttc actgaatcaa atgaacccat ctgtactgca ccgttactgt agctatatcc
    19021 ttcgcttgag tagccgacat atcccatatt gattttgtct gtgataccgc tatctgctaa
    19081 agtatcgaca aaacgatcga caccaatctt tacttcaccg atccgattat tgtcgttcat
    19141 acttcctgac cagtctacga ccaatacgag atcaagaggc gtgatatctt tttgtacatt
    19201 tccacgagca ttcaaataaa catcaaacaa tccgggtgta cttgtttcct ttgcatactt
    19261 tcgtatcgca tagtctgctt gattaccggt tcctccatat tcgatatacg aatgggtccg
    19321 atcattagga tctccattcc aagaatttat accatcccat tgatttgttc cgtttttatt
    19381 gccttgatga tcaagaacat ttgtatttcc tgtaggttgc caactggctt taggataagt
    19441 gccctgatca tctgttgtat attctgtagg aatcgtttga ttagatggca cactcacagc
    19501 tggcaaggat aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt cttttgatga
    19561 ttcttcttct atttcgtctg gctttggtac tgacacatct gtatctttct tattttctgt
    19621 ctcatcaata atgtcatatt ctttattatc gacgatcagc ttgtttgcct ggctgtggta
    19681 aagtgctgga tcgattggaa aactcagatc gatagtttgt ttagtgtttg atgagatttc
    19741 caacgtaatt ttcccgctat ttacctgata agcatagcca tctccttctt ttctttccac
    19801 atcacttaag taattttcga ctagaccaat ttctttttct atcttttcag cactggtgtt
    19861 attcgcattt agcttgatgt tcagtgcttt ttttccagac tgatccgaaa tgacttcagc
    19921 tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct gctattgcag ataatggcac
    19981 aaagtttgat aaactcaata ttatcagcat gaaaataact ttcagtttct tccctgtggt
    20041 cgtcattttt cccctccttt ttcttttttt ctgttttgac gtgaatagtg aaaattatta
    20101 aaataaacag gcgttacata attttattta ttttaatatg ataattatta tacgaagggc
    20161 gctaaaggga atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt
    20221 cgattaatga gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt
    20281 gttggaatga aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa
    20341 gtggatgact aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag
    20401 gtatatcgaa gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa
    20461 attggggtat cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat
    20521 gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac
    20581 gattgagtat tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa
    20641 acaatgcaat caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca
    20701 gttaactagc cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag
    20761 ttactacgga agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat
    20821 ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt
    20881 ctttattatg tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg
    20941 gaaagctgga gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt
    21001 gaaaaatctc ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt
    21061 caatacgatt ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg
    21121 aaaaaatatg tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata
    21181 tgttgttcaa ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac
    21241 tcatttgacg atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct
    21301 atatgctgga tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat
    21361 ttattattta tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta
    21421 tacttctgat aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat
    21481 tgaagaatat atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat
    21541 tactgaagat cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca
    21601 acccattttg aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa
    21661 tgaagaagaa ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa
    21721 ccaccctgat aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt
    21781 cagcaatcca acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat
    21841 ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa
    21901 ctgcttcttc cgacgattga atggagaaat gattgattct taataattcc agccattttt
    21961 cgttcattgg agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct
    22021 gggttaacaa gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact
    22081 ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga
    22141 tatcttgatg gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat
    22201 cttgaaaata tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga
    22261 ctttgacttg gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct
    22321 gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat
    22381 ctctgattat acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct
    22441 tcttctgtca agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg
    22501 acatcatgag taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat
    22561 ttgacgactt catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc
    22621 aagcttggct cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa
    22681 aggtctttag gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg
    22741 atttttgttg aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt
    22801 accgttaaaa aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt
    22861 aaatctgctc gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct
    22921 ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca
    22981 gaaggaccaa tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa
    23041 gctgagacct ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt
    23101 tttttcattt ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta
    23161 tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt
    23221 gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa
    23281 acaaaagagc aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt
    23341 tagcgatgaa gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct
    23401 gaagtgtcat cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt
    23461 atatcatcgc atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg
    23521 tcccaatagt taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat
    23581 cctttattac gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat
    23641 acttcaaagc agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag
    23701 tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat
    23761 ctccaatttt atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt
    23821 taaattcatg ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa
    23881 ctgtaacatt ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt
    23941 tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt
    24001 gatttgcatc ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa
    24061 actcttctga aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga
    24121 acataatacc gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta
    24181 gaaacatttg tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat
    24241 agtgtaactc taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt
    24301 ctaagacatc tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt
    24361 tatgctttcc atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta
    24421 ttagtagtag aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc
    24481 ggcgagataa cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt
    24541 tatgatcttg caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca
    24601 aaaatacgga aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg
    24661 gaagataaga ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat
    24721 cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat
    24781 gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat
    24841 aaagaacaag ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa
    24901 aataagggaa tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct
    24961 gatacgacga ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt
    25021 actggattgg ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg
    25081 tgatgcaatg aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt
    25141 agcagcattt gcttttatct tttttctttt gggattcatt actttacaga tattaaacca
    25201 atctgcatac agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt
    25261 acaattagag atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga
    25321 acaaaacaga acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac
    25381 tcaagtgatc ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg
    25441 tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat
    25501 tgaattaaat gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc
    25561 ggagacgaat caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa
    25621 gaattcatta gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct
    25681 ctctattggt atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg
    25741 gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat
    25801 tattcaaaat agtttggaac atctttttac caagcctaat catactatta ttgaagaatc
    25861 cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt
    25921 gctgacaatc gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac
    25981 acaagaatat atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa
    26041 agagtttatt ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca
    26101 tcaagttttg gttattttgc tggataatgc attaaaatat acgaatccag aagataagat
    26161 cattttggat tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc
    26221 ttctatcagt gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc
    26281 tcgggcgaaa gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga
    26341 acagcatcat ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt
    26401 ccaattgccg ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa
    26461 tcttgtcaca gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa
    26521 ataagccgaa atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac
    26581 gattttttat tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct
    26641 cggctaacat cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact
    26701 tcttcacgaa taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta
    26761 atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg
    26821 cgttcgatga cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc
    26881 acatgtttgt ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt
    26941 tcttctttta gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc
    27001 ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc
    27061 atattcaatg gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct
    27121 gttccagtgc cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt
    27181 gtatccattg tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat
    27241 tttccatcag aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc
    27301 tcagaatcat aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct
    27361 tcttcaccat ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat
    27421 cctagtctag atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct
    27481 tcgttacgca accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg
    27541 accgttaaat ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat
    27601 attgttactt ataaatttat tctataggag acgattgttt tctacaaata tttagattgg
    27661 aatttgatta aaacaattta attgaaattt gaaaacattt atgttaatga aagtcatatt
    27721 ctaataaaat gtcatttgtt tctatatgaa tttttatgaa aaatttcata taatttccac
    27781 tcgttgtgat ggtttaattt atccaatcca tcaataagca aacaacttat ataaaaaagg
    27841 attaattctc aatataattg ctcctaccat agaataaatc ttcatgtaaa gtaatgaagt
    27901 accaactatt tctaatactt ctttaagaat tatcccaaca atatcgttag acaaaccatt
    27961 attgaataaa tggagagcca atcgattctg aaaaaatgtt ttctaatttt tcggaaataa
    28021 aagaaagagc ctcctgccat tagaacgaac caaatccatt tcattgctca tgaagtaacc
    28081 acatagctaa aaagaggata aatcaattaa aagatcaaaa gaatgactgc actaataaaa
    28141 taactaaaaa tgtatcttct tacttcatat cttttagcta gtacttagtt aaaaatttgc
    28201 ttcctaagat caagacaaac tgtctatctt catttgatga aatgcggtaa aatatagaaa
    28261 atgaacacta ttggagggaa tttgtaatgg acagcaaaac tagaaaacaa ctgcaagaaa
    28321 agttaaagca acgtacacac ttgctccctt ccgtctttca acagttttta gaagaaaatc
    28381 gttcttccct ttcattaggc agtcgctatg aatacgcaaa agattttcat ctgtttactg
    28441 attatttgag aaaagtattt tctgaagaag tggatgatca gctgattatc catttagaaa
    28501 aacaagatta tcagaatttc ttagctaaaa tctatcgaca tactcgttcg ttccaaacta
    28561 cagcgaacca agaaacggag caattgtttg aaaatagcta ttacgggatt tcaaggaagc
    28621 aatattcatt aaatcatttt ttacgatttt tgtatcaaaa aggactgatt gaagaagaga
    28681 tttctctttt gggtacaagt ttaccggaaa caaccaaagc agctccgcga tatatcgatg
    28741 cagaactttt caaagatttt gagcatttgt ttatcccgat tgaaggcttt caaacaagca
    28801 gagaagctag ctttgaagaa aagaatagac cgaggaacct tgcaattact gccattcttc
    28861 tgtattgcgg attaaagatc catgaagtag tagaacttaa acgtaaagat gtatctttga
    28921 ccaagcagat tcttaatctg aatagaaaag aaaacagact gaataaagta ccaataccaa
    28981 aagaagcgat gccatttctg gagaactatt ttaagttaac agcacaaaat acggacgaaa
    29041 attcttttgt ttttcgttcc tcacatgatc agcaaatttc tcctcggact attcgccaaa
    29101 ttttgagcaa aattacagta tataaaaacg tatcgccaga actatgcaga aaaacatatc
    29161 gttattatgc agaactatat ttagatgatg ctgacgcggt aaattattta tgtggtaatc
    29221 gatacctctc atcccattct tttcaagata tctgggaaaa gatggttgat ttttcttatc
    29281 acgaattagc ggcattcgtt cgggtttctt ccatataaaa caaatgtatg atcttttttt
    29341 cacaataaac tatgttatta tataaaggat tatgttacga ttatcttgaa tttgtatatt
    29401 acttaacaaa agataatgga gtgtgaatga tggagaccat aacgtattcc cctttgaact
    29461 tatttacgaa ttttcaagaa gctgcagcta acttttccga gaccccgatt atctttgatg
    29521 agccactttc agctttccca gaattaggac ttgaaacaac ttacaaaaaa tgctgcgaaa
    29581 gtgtgttgaa acgggcttat caattggctc atcttggagt gaaatccgga gacaaagtta
    29641 ttatttataa gagttccgct tttgacacat accttctagc tgtttctgtg tcttaccttg
    29701 gtgcagtgcc tgtcatgact tcttatcatc taccaacaac aacaatggaa gtatttatcg
    29761 atcgtttaga agaccctttc attttatttg atgatgagac aaaaaagcga gtgagagaaa
    29821 tttcaaatgg aacaaaatca aaacagattc c
//
LOCUS       DACDRK010000029        28306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDRK010000029 DACDRK010000000
VERSION     DACDRK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.029"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IWJ77_001377"
     tRNA            8..81
                     /locus_tag="IWJ77_001377"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IWJ77_001378"
     tRNA            101..173
                     /locus_tag="IWJ77_001378"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IWJ77_001379"
     tRNA            201..274
                     /locus_tag="IWJ77_001379"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IWJ77_001380"
     tRNA            288..361
                     /locus_tag="IWJ77_001380"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IWJ77_001381"
     tRNA            385..474
                     /locus_tag="IWJ77_001381"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IWJ77_001382"
     tRNA            487..560
                     /locus_tag="IWJ77_001382"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IWJ77_001383"
     tRNA            579..654
                     /locus_tag="IWJ77_001383"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IWJ77_001384"
     tRNA            656..726
                     /locus_tag="IWJ77_001384"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IWJ77_001385"
     tRNA            740..813
                     /locus_tag="IWJ77_001385"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IWJ77_001386"
     tRNA            838..925
                     /locus_tag="IWJ77_001386"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IWJ77_001387"
     tRNA            947..1018
                     /locus_tag="IWJ77_001387"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IWJ77_001388"
     tRNA            1041..1130
                     /locus_tag="IWJ77_001388"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IWJ77_001389"
     tRNA            1143..1216
                     /locus_tag="IWJ77_001389"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IWJ77_001390"
     tRNA            1235..1307
                     /locus_tag="IWJ77_001390"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IWJ77_001391"
     tRNA            1311..1383
                     /locus_tag="IWJ77_001391"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IWJ77_001392"
     tRNA            1388..1458
                     /locus_tag="IWJ77_001392"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IWJ77_001393"
     tRNA            1471..1544
                     /locus_tag="IWJ77_001393"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IWJ77_001394"
     tRNA            1556..1627
                     /locus_tag="IWJ77_001394"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IWJ77_001395"
     CDS             1893..3086
                     /locus_tag="IWJ77_001395"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9453006.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IWJ77_001396"
     CDS             3216..4688
                     /locus_tag="IWJ77_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9453007.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IWJ77_001397"
     CDS             4771..5559
                     /locus_tag="IWJ77_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9453008.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IWJ77_001398"
     CDS             complement(5626..6294)
                     /locus_tag="IWJ77_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9453009.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IWJ77_001399"
     CDS             6409..8121
                     /locus_tag="IWJ77_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453010.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="IWJ77_001400"
     CDS             8124..9893
                     /locus_tag="IWJ77_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453011.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="IWJ77_001401"
     CDS             complement(10098..10664)
                     /locus_tag="IWJ77_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9453012.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="IWJ77_001402"
     CDS             complement(10664..11620)
                     /locus_tag="IWJ77_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9453013.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="IWJ77_001403"
     CDS             complement(11620..12276)
                     /locus_tag="IWJ77_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9453014.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="IWJ77_001404"
     CDS             12646..14664
                     /locus_tag="IWJ77_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9453015.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15091..17505
                     /locus_tag="IWJ77_001405"
     CDS             15091..17505
                     /locus_tag="IWJ77_001405"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9453016.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="IWJ77_001406"
     CDS             17853..19406
                     /locus_tag="IWJ77_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9453017.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="IWJ77_001407"
     CDS             complement(19787..21571)
                     /locus_tag="IWJ77_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453018.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="IWJ77_001408"
     CDS             complement(21586..23307)
                     /locus_tag="IWJ77_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453019.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="IWJ77_001409"
     CDS             23407..24168
                     /locus_tag="IWJ77_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9453020.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            25135..25403
                     /locus_tag="IWJ77_001410"
                     /pseudo
     CDS             25135..25403
                     /locus_tag="IWJ77_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            26075..>28306
                     /locus_tag="IWJ77_001411"
     CDS             26075..>28306
                     /locus_tag="IWJ77_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453021.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         8925 a         5059 c         5989 g         8333 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    24721 aaggttattg ttatttttat atattctttt taagaatgat tgttttttta tagaaatttt
    24781 aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata ttatagaagt
    24841 tgactggtag tgatttattt ttcgtcgttt tataaaagtg ttattttttg gaaaaattca
    24901 caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta tgactaatat
    24961 aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg gaatcaatga
    25021 aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat agcaatacat
    25081 cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac aacaaaagga
    25141 ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt tattctatac
    25201 aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa tatatggtac
    25261 agaatataga aattattgac attacagaaa atgatgaaga tttaatacag attgtcacta
    25321 tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg attaaggagt
    25381 tgatggaaga cgatacaagt taagaaagtg aaactacgcc caaataaaga gcaaaaagag
    25441 taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt ttattagtaa
    25501 tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg tttagttgaa
    25561 ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc caagtattct
    25621 aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt gatgaaggtt
    25681 aaccaataca gggattcact gatgaaattc tagagtctga ccttggtatc aacgatttag
    25741 tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa taactattag
    25801 ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca taattatggt
    25861 taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac agaagacaat
    25921 cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa ccgaaaatac
    25981 gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta tcgggtacct
    26041 tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc ataaactgtt
    26101 aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa cggttctagc
    26161 tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc aagaggaggc
    26221 tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc taaatgcatt
    26281 ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca atgattacga
    26341 acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg caagaagcgg
    26401 tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa gcacaagtga
    26461 gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag aagaaagctt
    26521 aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg aatctcagat
    26581 tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc gccccacttt
    26641 gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg gcgatattct
    26701 tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct taattgagtt
    26761 cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt accaagaagg
    26821 cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag ccgctcagtc
    26881 gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa
    26941 taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg aattttcaat
    27001 tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct ctagagaaat
    27061 cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga ccaacagtaa
    27121 taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag tggttaccga
    27181 tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt ccactggaaa
    27241 tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag gtgaagcaaa
    27301 cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta ataaggaaac
    27361 gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt ttattgaata
    27421 tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg atgggttttc
    27481 tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact accgcttgag
    27541 aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca aaaatgttat
    27601 caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt ctggaacaag
    27661 aaatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt catttctatc
    27721 agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt tcagtgcgaa
    27781 taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg tagaggcgta
    27841 tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca atcgattgaa
    27901 tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta aaactgatga
    27961 gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat
    28021 acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc ctgtaggtag
    28081 ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga cggtacatga
    28141 tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa acatccttga
    28201 tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc ttcaaggagc
    28261 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggt
//
LOCUS       DACDRK010000030        26870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDRK010000030 DACDRK010000000
VERSION     DACDRK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.030"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1087)
                     /locus_tag="IWJ77_001412"
     CDS             complement(<1..1087)
                     /locus_tag="IWJ77_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9453022.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKE"
     gene            1297..2298
                     /gene="ccpA"
                     /locus_tag="IWJ77_001413"
     CDS             1297..2298
                     /gene="ccpA"
                     /locus_tag="IWJ77_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9453023.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2635..4980)
                     /locus_tag="IWJ77_001414"
     CDS             complement(2635..4980)
                     /locus_tag="IWJ77_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9453024.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5259..6161
                     /locus_tag="IWJ77_001415"
     CDS             5259..6161
                     /locus_tag="IWJ77_001415"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9453025.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            6374..8149
                     /locus_tag="IWJ77_001416"
     CDS             6374..8149
                     /locus_tag="IWJ77_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9453026.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8215..9801)
                     /locus_tag="IWJ77_001417"
     CDS             complement(8215..9801)
                     /locus_tag="IWJ77_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9453027.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            10052..10276
                     /locus_tag="IWJ77_001418"
     CDS             10052..10276
                     /locus_tag="IWJ77_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9453028.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10418..12169
                     /locus_tag="IWJ77_001419"
     CDS             10418..12169
                     /locus_tag="IWJ77_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9453029.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12169..13953
                     /locus_tag="IWJ77_001420"
     CDS             12169..13953
                     /locus_tag="IWJ77_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453030.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14279..15130
                     /locus_tag="IWJ77_001421"
     CDS             14279..15130
                     /locus_tag="IWJ77_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9453031.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15184..16935
                     /locus_tag="IWJ77_001422"
     CDS             15184..16935
                     /locus_tag="IWJ77_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9453032.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            16939..17109
                     /locus_tag="IWJ77_001423"
     CDS             16939..17109
                     /locus_tag="IWJ77_001423"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453033.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(17149..18051)
                     /locus_tag="IWJ77_001424"
     CDS             complement(17149..18051)
                     /locus_tag="IWJ77_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9453034.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18282..19685
                     /locus_tag="IWJ77_001425"
     CDS             18282..19685
                     /locus_tag="IWJ77_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9453035.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19685..21118
                     /gene="ascB"
                     /locus_tag="IWJ77_001426"
     CDS             19685..21118
                     /gene="ascB"
                     /locus_tag="IWJ77_001426"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9453036.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21263..22189
                     /gene="trxB"
                     /locus_tag="IWJ77_001427"
     CDS             21263..22189
                     /gene="trxB"
                     /locus_tag="IWJ77_001427"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9453037.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22403..24130
                     /locus_tag="IWJ77_001428"
     CDS             22403..24130
                     /locus_tag="IWJ77_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9453038.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24202..24495
                     /locus_tag="IWJ77_001429"
     CDS             24202..24495
                     /locus_tag="IWJ77_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9453039.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(24587..25447)
                     /locus_tag="IWJ77_001430"
     CDS             complement(24587..25447)
                     /locus_tag="IWJ77_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9453040.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25539..25967
                     /locus_tag="IWJ77_001431"
     CDS             25539..25967
                     /locus_tag="IWJ77_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9453041.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26129..26305
                     /locus_tag="IWJ77_001432"
     CDS             26129..26305
                     /locus_tag="IWJ77_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9453042.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26332..26778
                     /locus_tag="IWJ77_001433"
     CDS             26332..26778
                     /locus_tag="IWJ77_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9453043.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8553 a         4786 c         5681 g         7850 t
ORIGIN      
        1 attcttttgg tgtagtcgtg aatggcacac agccatcgct tgtcacatat acacagtctt
       61 cgatacgtac ccctactttt tcaggaatat agattcctgg ttcaagagag aagcacatcc
      121 cttcttcgat tactaagtca tttccttcta caagggaagg gaattcgtgg acggttgttc
      181 cgatcccgtg acctaaacgg tgattgaaat attcgccgta gccatagctg ttgatcacgt
      241 ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg acggacggct tcttgtgcgg
      301 caagctgtgc ttcaagagtg atgttgtaga ttttttcttg gaagtcggtt ggtttttgat
      361 aagcaattgt acgagtagca tcactgcaat agccattcca aacaacgcca agatcgaata
      421 acacaagttc attcggtgaa atcttcgtat ttcctggtgt accatgaggg cttgcagcat
      481 ttgctcctgc aaggactaat gtgtcgaaag acatttgtga aacaccttgt tttttcaatt
      541 ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc ttctttcact gctttaaagc
      601 caatctcaaa tgcaacatcg gcccatttac cagcttcgat cagtttgtct ttttcctcta
      661 gagttttgat cagttgtaga cgttggatga tggaagttag atcagtgtaa gaatccgttc
      721 ccgtcaagat atcagagata gcatcgaagc gctcgacagt caaggcgttc ttttcaatcc
      781 ctacattgcc aggctgacca tagcgagcac gaagttcttt tccgatgatc tcccaagggt
      841 tttcgctgtc taaatagcca cggacatcat acgcccaact actgttttga gcatcttcaa
      901 cttccaaagc aggagtgaaa aggaaaggat cttgttcaag agggataaaa agagctagta
      961 cacgctcatg aggatcactt tcgtatccgg aaaagtaagc gatgtggcca gggtcgctga
     1021 tataagccaa ttcgacttgg tgttgtgcca tccattgacg tagttcattg atttttacgt
     1081 tattcatttg gcaacctact ttctaaatta attttgtatt caataattgt accactgatt
     1141 tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa attttcatga aaacagagat
     1201 aaaacggttg caattcttga gattatttga tattctattt tagaaatgta agacgtattt
     1261 tcagaaaaca tataaaatat gaataggaga acacaaatgg aaaagcaaac aatcactatc
     1321 tatgatgtag cgagagaggc aaatgtttcg atggctactg tttcacgtgt cgtaaatggc
     1381 aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg aagttatcga tcgcttggat
     1441 tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga aaacaacaac agttggtgta
     1501 atcatccctg atgtcagcaa tatgttcttt gcttctttag cacgtggtat cgatgatgtg
     1561 gcaacaatgt acaaatacaa tattatctta gcaaattcag atggcaacga ccaaaaagaa
     1621 gtcaatgtat tgaataactt actggctaag caagtggacg gcgtgatctt catgggtcac
     1681 catatcacag acgaaatccg tggagaattt tcacgttcaa aaactcctgt tgtattagct
     1741 ggctctattg atccagatga acaagtaggt agcgtaaaca ttgattatgt atccgcaaca
     1801 aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa aaatcgcttt tgtcagtggt
     1861 gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag gctataaaga agcattaagc
     1921 gaaaataacc taccatatag cgaaggattg atctttgaag cacaatacaa cttcaaagat
     1981 ggtctatctc tagtcgatcg gatccataat agcggtgcaa cagctgtcta tgtttcagat
     2041 gatgaattag ccatcggtat tttggatggt ttattagacc gtggcgtgaa agtaccggaa
     2101 gaattcgaaa tcatcacaag taacaattca ctactgacag aagtagctcg tcctcgttta
     2161 acaagtatca cacaaccatt atacgattta ggggctgtat ctatgcgcct attgacgaaa
     2221 atgatgaata aagaagaagt cgaagaaaaa acgattatct tgccttatgg aatcgaagaa
     2281 aaaggatcta caaaataata aaaggacaac cattcagagg ttgtgtgcct gccgtttaac
     2341 tctgcgttac aaggagcaat ttcaaaaaac agcatctcat gtttgatgaa tattgtgact
     2401 cgtgattcgg agttggcagg agaacaccgt ttatttggga aaaggggtta tgacaaaatt
     2461 ttgttataat cctttttttg tgtcttaaaa aagagagaaa gtggttgaaa ttacatttat
     2521 cgtatataaa acaatccgtg tatagatgct gcattttgtt caccaccatc aaaaaaataa
     2581 gggactgtga caatactatt gtcacaatcc cttattactg tgtattctat taaatcaatc
     2641 atctttctca tctgattctt tagttgcttg attcgctctt gcataagctg aagcggtgtt
     2701 ccagtaatct gtatagtttt catcggtacc gccaacgcca aatttaaagg cgcttttctt
     2761 cggtccattt ttcgcccata gcgattcaac tgtttttcct ggtgtttgga tggatttttt
     2821 attgacagta acttttccag gtgtttgacc agtaaatcca gatacttttt tcttcatcac
     2881 atcagatgat aattcgaatt tctcatcagt gccaaaaatg tcaggttttg cttggtaagc
     2941 ttgattgatc agattggcaa gataattagc cgaacgtatc cttgctttgc tgtccattcc
     3001 agtattgtca tcgtgccctg cccagctact gatggtaata gacggagtag agacaattag
     3061 ccatgaatct cggtattcat tggtagaacc agtttttccg acccaatctg cttttcctag
     3121 atttccgttt aagcttgaga tggcatcttt gaatggtgta gtgatttttg cattgatcac
     3181 actacgcatc atgtcattca tgatcgatgc cgttgcttcc gaatagatcc ggacagagtt
     3241 gctttcgtgt ttataaatta catttcctgc gttatctgtg atggaatcga tgatatagcc
     3301 ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg ttggtctgtt gaagcgtcgt
     3361 gacatctact gtacctaaag gagcagattc aacgccccaa ttgtcatttg ccgggtagtt
     3421 catctttgcc aagtagtgct cgtaagcata ttgtttcgat ccaccggaat tcaacacatc
     3481 ttgatataag tgataagccg ggatattgtt ggaccattct agtgactcac ggacggtttg
     3541 gaaggtgttc gatccttcgt tagtagcatt gacgattttc tcgccagcat tttctccttc
     3601 ttgccaagtt gttgcataat ctgatacacg ggattctgat ccgataagcc ccatgtcgat
     3661 tgcaggtcca taaacaagaa ctggtttgat tgatgagcca gcttgacgtt ctgtatcgaa
     3721 ggcatggtta ttttgattct gactgtaatt ccgtccgcca acgaatccgt agatgcggcc
     3781 agttctgtta tccatcaata catttccggt ttctacttga ctgccagcac catcatctaa
     3841 catgtatcca taattttcaa cgccattttg caatgcagca tagacatctt tatcgattgt
     3901 cgaatggatt gtatatcctt tattttggat cgtttgctgt gccttttgat aataagcgtc
     3961 actgaccgat gaatcagaca tatcagcgtt gtctttttca gccaattgtt gtgcaatgat
     4021 tcttgttgct tcattcatca cagtataata caagaattct ctgtcagtct gttctgcgat
     4081 ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt gcttcttcgt attgttcttt
     4141 cgtaatttgg ccttcacgat acatactgaa taaaacaaaa tcttttctgg caaggccggc
     4201 agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga ctatacgtga tcgggctttg
     4261 gggtaatcca gcgatataag cagcttgcgg cagcgtcaca tcttttgcat ctacaccaaa
     4321 gattcctttt gcagcttcct ggactccagc aatgttttgt cccttattat tacgaccaaa
     4381 tggtgagaca ttcaaatacg tagcaatgat ctcatctttt gaaaagtatt tttctacttg
     4441 tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt gtttcatctg taaggatctg
     4501 ctgtttgact aattgctgtg tcaaagttga tccaccagaa gagccaatac cagtagcttc
     4561 agatactaat gctcgtaaaa cagctttagg aacgtaacct tggtgtttat cgaagtattc
     4621 atcctctgtg ctgataattg cagttttcaa taaaggtgaa atcttatcag atgaaatagt
     4681 cgtgcgcatc agatctgtct ggatttttga tatctctgta ttatctgcat aaacaatctt
     4741 tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct tttttagtcg gcaattgtgt
     4801 gtcttcgacg agataggcaa aatagccagc gcctattcca atacctagag ctgcgaacat
     4861 caaaagaaga acagtaataa aaacagtcaa tgattgaaaa actctaaaaa taatcttagg
     4921 aacaaaccat ttttcttttt ttcttctatt ctgttttttt cgagtatttt tgcgtggcaa
     4981 aagggtcacc tcaactaatg gttttatttc gctaattata gcaaataata gaacaaaaaa
     5041 acagacaagg ctgtttttgg caggaatttc ttttttaaaa gagttaaaac gtgagaaaat
     5101 gatctttatt ataaaagagt tcttaagctt ttctttagag aaaatttcag ttactatgga
     5161 gaacagggca aagaaaaatc aatcattttc tttgtatatc atcttaccaa tatataggaa
     5221 ttgttagtaa caattgcctt tcaaggagga atccatccat gttattaggc tcacacgtca
     5281 gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga agcagccagt tatggatctt
     5341 cgacttttat gatctataca ggcgcccctc aaaacactag aagaaaagcc atcgaagaga
     5401 tgaatattga tgccggaaaa gcgtacatgg cagaaaaagg tctaacaaat atcgtcgttc
     5461 atgcccctta tatcattaat ttggggaata cgatcaaacc agaaatgttt ccttttgccg
     5521 tccaatttct gagagaagag attgttcgtg cggaagcttt aggcgctact caaattacac
     5581 ttcacccagg tgctcatgta ggggcaggaa cagaagccgg aattgctaga attattgaag
     5641 gcttgaatga agttttgaca aaagaacaga cggcacaaat cgctttggag acaatggcag
     5701 gtaaaggtac ggaaatcggt cgaacatttg aggaattagc gcaaatcatc gatggagtaa
     5761 aattgaatga aaaattatcc gttacttttg acacctgtca tacgaatgat gccggttata
     5821 atattaaaga agacttttca ggtgtaatgg aagagtttga taagatcatc gggttagatc
     5881 ggcttaaagt actccatatc aatgattcta aaaatccgca aggttcgcat aaagaccggc
     5941 acgccaatat tggttttgga acaatcggat tcgacacgct gaataagatc gtccatgatc
     6001 caatgtttga caatatttcg aaaatcttgg aaactcccta tgtaggcaaa gataaaaaga
     6061 aagcttacgc accatatgga aaagaaatcg ccatgttcaa agcgcaaaca ttcgatccag
     6121 aagtgtttcc tgagttatta gcagaagaag tgatactcta agattttcta tctgacagca
     6181 gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt taagaaacaa aggagtactt
     6241 tttgcagtct ttttcatcat gcatcttctc ccccctcttt ttaaatttag acaaacaagg
     6301 tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag caagtggcaa aacgtgattg
     6361 gaaggacgaa aagatgctga acactttaaa acccttgatc aaccaactaa aaaagaaaca
     6421 tacatacttt attcttggca tagccctatt atttacaatt attcgtttgt atttatatag
     6481 taaagcaatc tatggaacag acatgtcggc aacctatgat gaccagctgc tgattcgcta
     6541 tgctcaaaat attttggacg gtaaatggct aggggagtat acgactacaa cactttccaa
     6601 aggaatctcc tacagcttat ttttagtact tgcacataaa ctgcatcttt cttatgcagt
     6661 tttgttcggc atgttcagta tttttgccag tatattggtt gcactcgcgt ttaaaccgat
     6721 tgtgaagaac aagtatcttc ttttaagtat ctatttttat tttttgtttt ctccgattag
     6781 ttttacacat gaatattcca ctcgaacata tcgtaatgcc ctcgtgattc cggctgttga
     6841 gatcattatc gcttgtttat tagctattta ttaccgcaaa tacggttcta gcagaaaatt
     6901 gctgccatgg tttttagggc tttctgtcgc ttttcctttc ttttggttca ttcgcgaaga
     6961 ttctatctgg cttcttcctt ttactgtagt agctttgggt atcagtttcg tccagatctt
     7021 gctagggcaa gacttttctt tcaaagaagt aaagaagtta aaaaaacgac tctttcttct
     7081 ctcgaaacgg gaatggctga aactttcatt gtttttactt cctatagttg gcttttccat
     7141 cgtctatcaa tggatcgaat ataaaaatga gacaacatac ggtattgcaa ccatcaatga
     7201 ccgttccagt ggagaatttg gacgtttgat gaaacaactg atccgaatag atgatgaaac
     7261 gaacttgaat aaagaaaaca gtgagatatg ggtctcacat gctgcactaa ataaagcatt
     7321 agccgcatct cctacattcc atactgtttc tacccgtatt gacgaattat atacgactca
     7381 cgcgtggact caaggtggaa aagtaaaaga attacccgga gatatcatct tttgggcatt
     7441 gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat gcaaaagaaa ccaatgattt
     7501 ttggaaaaaa gtcaaccaag aattagaaga agcctatcac aagggaacaa tcaaaaagaa
     7561 aaaagagttc tatctgatgg gatcaggaga tggaaagacg atgagtgatc tgccaatcgt
     7621 attcgatttc cttcaatcag gatacgctta tgcatggcga tataaagatt tccatcaagg
     7681 aggaaattat agtttcggaa cgcaagaaca agtcaaacag gctcaagaat tattaaacct
     7741 tccgctaatg gagaattgga tcgatagtaa tgaaccgaga acacctaatt tccaattaac
     7801 aaaatcagca aaagtcgcga atgtcatcat aaaaatttac caagttctgg ctattcccgt
     7861 gctgattgct tcaatagcag ggatattact tatgatacta ggaaccgtat ttgcaaaagc
     7921 tgatcgtgcc tattatagtt cctctttgat tgtggtcacc ggattaatac tgacacaatt
     7981 ggtttttctt ttcggtgtct catggttttg ttcttatgcg ccagaaaaaa aggactattt
     8041 tttaagtgtc tacactggtg caggcgtgcc attaacgcaa ttcatcgtag taacaagcat
     8101 gctagttttc agtaaattaa atctgaaaaa agctttatct tctaaataaa aagacccttg
     8161 gtagctagat ttttaaccta actatcaagg gtctttgtgt ggaaaaatca gtttttattc
     8221 gatttcttct accgtttcct gcaaaagctg ttcatatttt ttgtttcctt tatataaatc
     8281 accaatcgtc catgccaaca ggagaaggac aagaacaatg attacataag cgatgtgatc
     8341 agccaatgct agttgagcgg aagtggcttg ctcgccgaag aaactttcga tctgtcctgg
     8401 taatcctcgt aagttcaagt aagttagact aatcacagag aaccagccaa gaagttttac
     8461 gatgaatgaa tttttaaatc ggctacccat ctccgcttgg ctgttggtca tcatcagtaa
     8521 aggaatcatc gaaaacggca acgcaaaagc caagaacacc tgggattcat tcattaagtt
     8581 attcaaagcg gcatgttctt cgatcgttcc ttttgtactt gtaaacaaaa cacaaatcaa
     8641 tacaggaatc acagaaagca atcgtgtgac cagtcttctt agccaaatcg ggatacgcat
     8701 atggatgaat ccttccatga tgacttgacc ggtcaacgta ccagtaatag tagagttctg
     8761 gccagaagca agaagtgcta cagcaaataa agtggacaag ataccagatt ttgctacagc
     8821 aatcaaaata ccattgctca tagctgatgg atcagataaa gcttcataca gaccaaaaaa
     8881 ggaaggatct tggacagaac cggttttgaa gacagcgaca cccatgatca atagcagtgc
     8941 attgactaga aaagccattg acaactggat attagaatcc caagtagaaa aacgtacagc
     9001 ttgtgctaca tcttcttcgt tttggtgatc gatcttccga gtttgtgaaa ctgctgaatg
     9061 aagatataag ttatgcggca tgactgttgc accaataatc cctaaagctc cagtcagcgg
     9121 agtttcgccg ccgatgaccg gtgagtctga gaatgtatga ctgttaggga taaagccagc
     9181 aaataaactg tgccaatctg ggttagacaa gccaacttga tagacaaaaa cgaataaaat
     9241 gacgaaaatt aagcagacaa ccagtgcttc gatcttacga aacccgactt ttgtcagtag
     9301 taagagaacg aaaacatcta atacagtaat aaagacggca atcaccaatg gaatatcaaa
     9361 aagtaagtag agggcgattg ctgcaccaat gacttctgca atatctgttg ccataatggc
     9421 taattcagtc aagatccata aaatgacacc taatgtctta cttgtgcgtg ctcggattgc
     9481 ctgtgccaaa tccatttggc tcacgattcc taattttgct gccatatact gtaaaagcat
     9541 agcaatcaga ctagacatca aaataactga catcaataaa tattggaaat tttgtcctcc
     9601 ggtgatcgaa gtagaccagt tacccgggtc catataccca actgctacta acgcaccagg
     9661 tccagagtaa gcaagaagtg ttttaaaaaa acttttcccc tttggtacat caactgttcc
     9721 atttacttca gctaaagata aaccatttgt atgttcaata agtcgatgct tatgaaattg
     9781 tttattggat tgattattca agaaaaaacc acctttccta tttatctacg attacattct
     9841 acacctcaaa aagaaaaaat taaagaaaaa gattcggtac gcctaatttt ttttcatggc
     9901 gcgtcttgct agatgacgaa agaaattttg ttctcctcta aaaaaaccac tattttttgc
     9961 atgaaactaa agaatacgcc tttctttttt caataaactt tagtaaaata agtcattgag
    10021 agcaattcgg aaatagaaag gagagattta tatgttatca acaggtattg tcgtacttat
    10081 tgctattatt gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc gtaaatatat
    10141 gaaagactat ttagaaaaaa atcctcccgt taatgaggac atgttgcgat caatgatgat
    10201 gtcaatgggg caaaaaccat ctgagaagaa aattcgccag atgatgcagc aaatgaagaa
    10261 ccaagggaaa aaataggttc tgacccaacg actttttacg ggctgatttt ttcagtccgt
    10321 tttttatttc gtaatttgtt agactattca gattattcag caaaaaagac aagcacagac
    10381 aatcctaggc tcttgataaa gacaaaggag gagtaaaatg tcgattttta gaaaattagg
    10441 ctggtttttt cgtcaggaaa agaaacatta tttaattggg gttttctcgc tattcgcagt
    10501 ggccttggta cagttagttc ctccaaaagt gattgggatc atcattgatg agatcgccga
    10561 taaaaattta tcaataaaga tcattttcgg gtggatcttc ctattgatta tcgctgcttt
    10621 tttacaatat ctttttcgtt atatctggcg gacgaatatt tggggaagtg cagctcgatt
    10681 ggagaaagaa cttcggcagc agttattcaa acattttaca gaaatggaca atctctttta
    10741 ccaaaaatat cggactgggg atttgatggc tcacgcaacg aatgatttga atgcgatcca
    10801 gaatgtagca ggagctggta tcttgacctt tgcagattca gtgattacgg gcggaacaac
    10861 gatcattgcg atgattttgt ttattgattg gcgcttaaca ctgattgcgc tgattcctct
    10921 gcctttgctg gccgtcactt cacgagtgct ggggtcaaaa ctccatgatg cttttcgtga
    10981 ttcgcaagaa gctttttcta gtatcaatga taagacacaa gaaagtatca ctgggatcaa
    11041 agtcattaaa acatttggac aagaaaaaga agatctagcg gattttgaag aaaagattga
    11101 agatgcaatt attaaaaaca aacgagtcaa ttttttagac tccttatttg atccattcat
    11161 taccttgatt atcggtctgt cttatgttgc aacaattatt ttaggtggaa agtatgtctt
    11221 ggaaggcacg atctcgatcg ggcagatggt ctcatttatt agttatatcg gtatgcttgt
    11281 ttggccgatg tttgcgattg gtcgattgtt caacgtattg gaaagaggaa atgcttctta
    11341 tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc gtggaacaac tgggtgcagt
    11401 gcagaccccg gcaaaaggcg agctgaatgt agctatttcc tcttttaatt atccgaaaga
    11461 agaatcacca gcattagaac aaatcaagtt tgcgatccaa gaaggagaaa cattaggtat
    11521 tgttgggaaa actggtgcgg gaaaaacgac gatcatcaaa ttactgatgc gtgaatacga
    11581 tgattatcaa gggaaaatcc aatttggcgg acgttcgatc aaagaatatt cattgaatgc
    11641 tttattagga tcaatcggct atgtgcctca ggatcacttc ttgttttcga tgaccattcg
    11701 agataatatc cgtttttcta atccaaaact ttcagaagaa gaagtagagc atgcggcttc
    11761 tttagcattt atcaacaatg aaatcaagga attaccaaaa ggttatgata ccttagttgg
    11821 agaacgtgga gtctctttat ctggcggaca gaaacaaagg atttcgattg ctcgagcatt
    11881 gatcaaaaat cctgaattat taatattaga tgatgcactc tctgcagtag atgccaaaac
    11941 agaagaagct atcttgcata atttaaaaga aataagaaaa gagaaaacaa ctatcattgc
    12001 agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc attgttgtcg atcaagggaa
    12061 aatcattgaa cgaggaaccc atcatgagct gcttgcacta ggcggctggt atgaacaaat
    12121 gtgggagaaa cagcaattag aagcgaagat agaaggaggc gaggcctgat ggatgaaaat
    12181 tatcaatcgg aatggacaaa atcgattcca cttaaagaac agaaaaagat catcaaacga
    12241 ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg ttgctatctt atttgcgttt
    12301 gctttgtctg tgatcaatgt cctattgcca aggatcatcc aaacttttat ggatgatcat
    12361 ctggctaaac agtcggctac gacacaagtt attttgtttt ttgctggtat gtacttattt
    12421 ggagtcatag taaaaagtat catttggttt ttccaatggt atttgtattc catggcctca
    12481 ttgaagacct atcagtatat tagggtaaaa ttatttgaaa agctgcatac actaggtatg
    12541 cgttattttg atcagacacc ggcaggatca accgtttctc gagtgacgaa tgacacagaa
    12601 acgctatttg aattttggta cgtctttttg atggtattga ctgggatctt tgcagtcgtt
    12661 tcttcattcc ttgccatgtt tcaaatcaat ggagaaattg ccttgtggac tttggtgttt
    12721 ttgccgattt taggaatcgt catctggtat taccagaaat tcagttctcg tatttaccga
    12781 aatatgcggg aaaaattaag tcagctgaac acgaaattga atgaatcgat ttccggcatg
    12841 cgtattatcc agcagtttcg ccaagaagct cgtttagcta aagaatttga acaagtgaac
    12901 aatgagtatt tagacacgcg ttatgcgatg atcaaaacga attcgattct gttaagtccg
    12961 atcatcaatt ttctatatgc attggcgatt gcgttggtgt tgactttgtt tgggatcgat
    13021 gcgttgcatt ctcccgtaga agttggactg atctatgcat ttacgaccta tgtccaggga
    13081 ttttttaatc caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt
    13141 gctggtagtc gtatcttgaa gattatggat acggaagaat atgcaccaca acaaaaccct
    13201 ggagcagatc aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat
    13261 gatggagagc acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt
    13321 gcattagtgg gacacaccgg aagcggaaaa agctcgatca tcaacgtatt gatgcggttt
    13381 tacgaatttt atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg
    13441 gaagagttac gtaagaaaat ggggctggtc ttgcaagatg ctttcttgtt ttatggggat
    13501 atcgcaggaa atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg
    13561 gctgagtttg ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa
    13621 gtgatcgaac atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga
    13681 acaatcgtga ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact
    13741 gaaacagaag gattgatcca agaaggattg gcaaaaatgc gtcaaggacg gacaacgatt
    13801 gcgattgccc atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa
    13861 ggtcatatag tagaacgcgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca
    13921 gatatgtaca aactgcaaag cagtggtata taaaagatgt tgtgacagta gaattttgac
    13981 ctaagcacta gagtgaaaat aaaacatagc ttctcatatt tttcatctgt gagagttagt
    14041 gtcgtagtca ccagatttaa aaaaagattc gacagctagt cagaagtgcc taatagccac
    14101 taagaaagcg ttgacaaaag catttgcttt cgataatata aagaaaacga tacaacttgg
    14161 atagtgattg cctgatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt
    14221 tttgggtttt tctttttgtt ttggatacag ccctatacat catggaaagg agcaaaacat
    14281 gctgattgag aaagtactga ataacaacgt cgtaataacc agggatgaaa gaaatcagga
    14341 aatgatcgtg atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga
    14401 ctcatgcatc gacaaaaaat ttcagttgac aaatgacggg ctttcacaac agttccaaga
    14461 attggcacaa gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac
    14521 aaaagaggct ctacaagtcc agttgaatga ttctgtgtat attgcattga cggatcattt
    14581 gtatacggct atcgaacgtc acaaaatggg aatatttgta ccaaatgtat tgctgtatga
    14641 tatcaaacaa ctatttccta aagagtatgc catcgggaaa aaaacagtga agaaaatctc
    14701 ggataaatac cacatcaagt tatcagaaga tgaagccgga tttatcgcat tgcatttagt
    14761 caatgcacaa ggcattcaaa gccagcaaga caatacctac ctgtttacta taacaataca
    14821 ggatattcta aatatcgtcc gctattacta taagattaca tttgatgaag attcagtcca
    14881 tttttttcga ttcgtcacac acttacgttt ttttctgaca cgcgtcctag atggagaaaa
    14941 atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt attcaaaaaa aatataagga
    15001 tacgtttggt tgcgttgaga aaatagcaga ctttttagcc gagacgttca actaccagat
    15061 gtccacggac gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg
    15121 ttgtaaataa aaaaaggagg ataaattaac aagaacgtat gaaataaggg tagtgacatt
    15181 cagttggcta aaccttccac taataaattg gagggaatag aaatgggaaa ataccaagaa
    15241 ttagcgagaa aaatcgtaga aaatgtcggc gggaaggaaa atatcaatgg tttgacacat
    15301 tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa aagcaaatga tgagatcttg
    15361 aaaaatatgg acggtattgt aacggtaatg cgagcgggag ggcagtatca agttgtcatt
    15421 gggaatcatg tgccagtcgt gtttgaagag gttataaaag caggaaacct tacttttgat
    15481 gaggctgttt caacagaaaa aatgagaccg tttgatatgc ttattgatgg aatatcgggc
    15541 tgtttccagc cattccttgc tatcctagct gctggcggga tgattcgtgg actgacagca
    15601 ttccttgtct tcttaggcgc gtttgaccgc ggttctggca catttgttat gtttgataat
    15661 atcggtgatt ctgtattcca atttatgcct gttattatcg gtttaacagc tgcccgtaaa
    15721 tttaaagtaa atgaattcgt agggatgttg cttggcgcag cattaatgaa tcctagcttg
    15781 tcgctggaag ctttgagtgg tgcagcagaa gcaccactaa cgaccatatt ttctggcact
    15841 atttttgaag cacctattta tcaaacagta ttcggcattc cctggattgc gcgtaactac
    15901 gcttcttctg tcattcccat tatctttatt gtgttgttag ctagccaaat tcagaaacca
    15961 attaaaaagt tagtacctga gatgatcgcg aatttcttcg ttccattctt caccgttctt
    16021 attacaatgc ctttaggttt tttactcgtt ggaccagtat tcacattcgc aactgatatt
    16081 ttaatggctg gatttgaaac acttttagca ctttcgccag ttatttatgg agcaatcgta
    16141 ggcttcttct ggcaaattct ggtgatgttt ggtctccatt gggcaatcgt accaatggga
    16201 ttgatgcaat tttctgtgaa tggttggcaa aatattatga cacctgttgc agtagtgtct
    16261 tttggacaaa ctgcagcact taccgcactc tactttaagc ttagaaatcc aaaagacaaa
    16321 gcaattgcaa taccagccat tgtttctggt attgtgggga ttacagagcc agctatttat
    16381 gggtttacat taccacgtaa aaaaatattt atttttacct gtgtcggtgg tgcgttaggg
    16441 ggcgcgtata gtgggttaat gaatttaact tcttggaatc aaggtggact aggcatcttt
    16501 acaattccca actatattag accagatggt gatttaacag atgtgattaa tgtattgatt
    16561 gggatcgcca ttgcgatggt ggtatccttt acgctgactt ttttcttttg gaaggatgaa
    16621 gcaggtgaaa cagatgatat acagaaaaaa tcaggtaaag aaattgtaaa aacaccgata
    16681 caaggacaaa tcgctccact aaatgcagca aaggatgcag cttttgcaca aggaacacta
    16741 ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac gtgccccttt tgacggaaca
    16801 gtcatgaccc tgttcccgac gaaacaggca attggaatgg tttctgaaac agggctggaa
    16861 ttgctgattc atgtcggatt ggatactgtg cagttggaag ggaaatattt tgaaagctta
    16921 gtgcagcaag gataaagagt gaaacaaggc gatctgttgc tacgcttcga caaaaagaaa
    16981 atcgaaaagg aaggatacag tttagaaaca cctgtgatcg tcaccaatta cacggattat
    17041 ttggatattt tggaaaacca aggggagacg gtcgggccag acgatgcact tatcaccgca
    17101 ctgacctgaa cggttaggtg aagaatggac taggcttaac tattcttttt attccacttc
    17161 tttattttca aaattgaaag gctctttcat gatggctgag ctgatagggc gtttatcgaa
    17221 tttttgaccg acagaaatta gatggactaa attctgttga taattttctg caaaaacaca
    17281 actatataac acgtctagaa gataacaaat tgatgtattg atcgtaaaat taccaatttt
    17341 tgaatacagt cgctcgcgtg tcgtaagtcg caaagcggct gtactcattg cagatagcgt
    17401 tgaatcaccg atactggtca aagctaaaat tggaacatga ttttgtttca atatttctgc
    17461 ggtacgaagg atcacattgt tttcacccgt ataagaaatc agaatcccgc acatatcact
    17521 cgtgcaattg tacgcttcat agaatgattc accaaaagtg gtgacaatcg tgacatccat
    17581 tttgatgcgc ttcattttca gcatgaaatc ttgagaaatc aaagtattcg agttactggc
    17641 gaaaatcacg attttttttg cttgaagcat tagttgtttg gctttttgca gttggtcatg
    17701 atgaatgaga gaaagtgtat catgaatcgt catctgttct aaagaagcca ttttatttgc
    17761 gatcgtcata ataccgtctg tggtggaaaa aggaagattc gcatctacgt cttgaaaatt
    17821 cccttcaaga taggctgttt cagctaaaaa agcattacga aaactgaccc agccatcaaa
    17881 acccattttt tttgctacac ggatgagtgt agaggggtgg acataacatc gctcagcaat
    17941 cgcttggatg gtcatttcgc caagtgctgg agaatcgctt aagagaaact ggatcacttc
    18001 attttctgaa ggagaaaagg agatttcttt gattttttca cgtaataaca tgtagatacc
    18061 ttcttcttat ttttttacgt aaattataac ttttttgaaa atgatttgta aacggattta
    18121 tactatataa cttaaaatag acattttttg acaagatatt tacaaaaatc cactagaaaa
    18181 cagtaaattt tgtaaatgtt actggaaaga aacagatttc tattgtttgt tttgtttaca
    18241 aaaaagataa tgaaagtgta aaaaagtaaa ggagtgagaa catggcaaaa ggaatcaaaa
    18301 tcgctacgat tggtggaggc agcagttaca cccccgagtt gatggaagga tttatcaaaa
    18361 gatatgagga attaccgata cgagaaatct ggctcgtaga tgtagaagaa ggaaaagaaa
    18421 agcttgagat cgttagtcag atgtctcagc gtatgtggga tgcttctccg tatgaagtca
    18481 agatctatgc aactctggat cgggaagaag cattgaaaga tgcggatttt gttacgactc
    18541 agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga gcggatccct agttattatg
    18601 ggatgattgg gcaagaaaca aatggtgctg gcgggatatt caaagctttt cgtaccgttc
    18661 cagtcgtttt ggcaatcgtt gaggacatga agcgtttatg tccaaatgct tggctgatca
    18721 atttcgcgaa cccaagtggg atggtgacag aagcagttat cagatatggc aaatgggata
    18781 aagtgattgg attatgcaat gtgccagtga tggcgatgat gacagaacca gcaatgcttg
    18841 gcaagaaaaa agaagagctg atctacaagt ttgccggtct aaatcatttc cattggcaca
    18901 aagtagcaga taataaagga aatgaccgca cggcagaatt gattgatcgt ttatacgatg
    18961 aagagaatgg attaccgaaa aatatctttg atatcccgtt tttcaaagaa caattaaaac
    19021 aaatgaacat gatcccttgt ggctaccacc gctattacta tcgtgaagaa gaaatgctgg
    19081 aacatgcttt agaagaatat caaacgattg gtacaagagc gcagcaagtg aaagaaacag
    19141 aagcagagct atttgaatta tacaaagatc cagaattaga ccataaacct gaacaattgc
    19201 aaaaacgagg tggcgcttat tattcagacg ctgcctgtga aacgattgct tcaatctatg
    19261 gaaataaaga aacacagatc gtggtatcta caaagaatga tggagctgtt cctgacctgc
    19321 cggcagattg tgtagtagaa gtcactgcct atgttggcgc acaaggagcg agaaatgtag
    19381 cgtttggttc tcttcctgct gctgaaagag gctggttgca agtaatgaag aatatggaat
    19441 tgctgacgat cgaagcagct gtgactggtg attatgggac agctttacag gcgtttacga
    19501 tcaatccact tatacgatca ggaaagacag caaaacggat catggatgag ttgtttattg
    19561 cacaccagca gtatttaccg caattcaagg agacaatcag ccgattaaaa gaagaaggaa
    19621 tcgaagttca agatgaactg gcaagagaat tatcaggtaa tacacatata atggaggaag
    19681 tataatggga ttcagaaaag attttttatg gggcggtgct acagcagcca atcaatgtga
    19741 gggcggttat gatgaaggtg gacgcggact agcaaacgta gatgttgtgc caatcggaaa
    19801 agaccgtttt cctgttatca ccggaaagaa aaaaatgttt gaatttgatg aggatcattt
    19861 ttatccagca aaaattgcaa tcgatatgta tcatcgttat aaagaagata ttgctttgtt
    19921 tgcagaaatg gggttcaaga cttatcgaat gtccatcgcc tggagtcgta tttttccaat
    19981 gggggatgag aaagagccga atgaagctgg cttgaaattt tatgaggatg cctttaaaga
    20041 atgccaaaag cacggaattg agccacttgt gacgattacg cattttgatt gtccaatgca
    20101 tttgatcaaa gaatatggtg cgtggcgttc cagagaactc gttggttttt atgaaaatct
    20161 gtgtcgagtc atcttcaatc gctataaagg acttgtcaaa tattggctga cattcaatga
    20221 gatcaatatg atccttcatg caccattcat gggcgcaggg ctgtattttg aaaaaggaga
    20281 aaatgaagaa caaatcaaat atcaagcggc acatcatgag ttggttgcaa gtgccatcgc
    20341 aacgaagatt gctcatgaag tagatccgga gaacaaagta ggctgtatgc tggcagcagg
    20401 agcatattat ccatacactt gcaatcctga agatgtttgg gcttctagaa aatcagatcg
    20461 agaaaattat ttcttcattg atgtccagtc acgaggagaa tatcccgctt atgcattgaa
    20521 agaaatggag cgaaatggca ttcagatcga aatggaacca ggggatgccg cattgctgaa
    20581 agaacatact gttgatttta tctctttttc ttattatgct tcccgagttt cgactaccga
    20641 caacgaactc ttggaacaga ctgcaggaaa tatctttgct tctgtcaaaa atccatatct
    20701 gcaagcaagt gaatgggggt ggcaaattga tccattaggc ttgagaataa cgatgaatga
    20761 tatttatgat cgttatcaaa aaccattgtt tattgtagaa aacggcttag gtgcagtcga
    20821 tacgccagat gaaaatggtt atgtggaaga tgattaccga atcgattacc tggcagcgca
    20881 tatccaggcg atgaaggatg cagtagaact agacggagtc gatctattag gctatacgac
    20941 ttggggatgt attgatttag tatcagctgg taccggtgag atgaagaaaa ggtacggatt
    21001 catttatgtc gatcgtgata accaaggaaa tggcacatta gcccgttcaa agaaaaaatc
    21061 attcaactgg tataaaaaag taattgattc aaatggagaa aatctttcga acgattgatc
    21121 aatgatttat aaaagtctat tgtttttcag cggatttttc gagtataata aagctattcg
    21181 agaaacgcaa gcagaacaat agactttttt tcgcattggc tgtaaagtat gcttacttga
    21241 ttaagtaaag gagaatctac atatgtatga tgtaatcgtt attggcgcag gtcctgctgg
    21301 gatgactgct gctttatatg cttcacgttc aaacttatcc gtattgatga tcgaacaagg
    21361 tgcccctggc ggccaaatga acaatacggc agaaatcgaa aattatcctg gattcgattc
    21421 gatcatgggc cccgatctag cttataagat gtatgaaagt gtctcaagat ttgggacaga
    21481 aaatgcttat ggaatcgttc aagatatcaa ggatcacggc agccataaag aagtcatctg
    21541 tgaagataaa agttacgaag gaaaaacagt cattatcgca acaggatgta tccaccgtaa
    21601 attaggcgta cctggcgagg aagaatttgc tggacgaggc gtttcttact gtgcagtctg
    21661 tgatggtgcg tttttccgta acaaacgtct agtagtcatc gggggtgggg actcagcagt
    21721 agaagaagcc atctatttga cacaatttgc ttctgaagta gtgatcgtcc accgtagaga
    21781 tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt gcaaatgaaa aaatttcctt
    21841 tatgtgggat acagtcgttg acgaaatcgt tggaaacgaa atggtagtga caggtgtgaa
    21901 agcacgtaat gtcaaaaccg gcaatgaatt tgatttagca gcagacggag tattcatcta
    21961 tgttggactg gatccattaa ctgcaccttt tagagaagca ggtttgacga acgaagctgg
    22021 ctggattcca acagacaacg aaatgaaaac gaatatgcca ggcgtttttg ccatcggaga
    22081 tgttcgtgaa aaagatttgc gacaaatcac tacagctgtt ggtgacggcg gtattgctgg
    22141 acaacaagtg tataaatact tggagtcttt agaagaaaca acagcataag tttaaaaaga
    22201 gtagaagaac gggttcaatt gttcttttac tctttttttt cgcgtgaaat ttactgaatc
    22261 tgatttatgc gaacacgtcc aacacctttt gataaaagaa tcctttcttg atttaagcgt
    22321 tttttggtcc tcatactttc atatctaata agtgaatgct atacttatta agaaccatat
    22381 ttcagaaaga ggtgttattt tcatgtcttg ggaacaagtg tatgaacaat gggtacatga
    22441 agaaaattta gaagaaaact taaaaaaaca attaaatgat ctgggtgaag atcctgaaaa
    22501 acttgaagat gctttttatg caccgttgga attcggtact gcaggtatgc ggggaatctt
    22561 aggacctggg atcaatcgaa tgaacatcta tacggtaaga caagcaacag aaggactagc
    22621 acgtttcatg gatacacaag gtgaagaaac aaaacgccga ggtgtcgcta tcgcttatga
    22681 ctcacgccat cagtcaccag aatttgcaat ggaagcagcc aaaacactag cacatcacaa
    22741 tatcccgtct tatgtattcg aaagtctgcg tccaacacca gagctttctt ttgcagttcg
    22801 tcatttgaag acgtttgcgg gaatcatggt aaccgcatct cacaacccag cggcttacaa
    22861 tggatataaa gtctacggag aagatggcgg acaaatgcct cctgcagacg cagatgcatt
    22921 gacgaaatat gtccgtgaag tcagcaatcc gctaaaagtc gaagtattat ctgacgaaga
    22981 agctaaacat agcgggttga tcacgatcat cggagaagaa gtggacgctg cttatctaga
    23041 agaaatcaaa gcagtgacga ttgatcgtga attggttgaa acaatgggga aagatttgaa
    23101 acttgtgtac acgccacttc atggaacagg gaaaatgttg ggcgaacgtg cgttgaaaca
    23161 agctggattc gaacaattcg tactagttcc cgaacaagct gtggcagatc cagatttcac
    23221 tacggtcaaa tcgccaaatc cagaagaaca ttcagctttt gaatatgcta tacgacttgg
    23281 agaaaaagaa ggagctgact tgctgattgc aacagatcct gatgcagacc gtttaggcgc
    23341 agcggttcgt ttgccagatg gttcttatga agtattgact gggaaccaaa tcggagcatt
    23401 gtttgctcaa tatattttgg aagcacatca atcaagaggc actttaccag aaaacgcagc
    23461 ggtattgaaa tcaatcgttt ccagcgaatt gcctacagca attgcggaac actacggtgc
    23521 taccatgttc aatgtgttga cagggtttaa attcattgct gaaaaaatcc aacaatttga
    23581 agaagaccat tcacatactt ttatgtttgg attcgaagaa agttatggtt atttagttaa
    23641 accttttgtt cgcgataaag acgcgatcca agcattgctt ttattagcag aagtagcagc
    23701 ttactataaa aaacaaggca aaacgttgta tgacggcttg caaacgattt ttgaaaccta
    23761 tggctatttc gcagaaaaaa cgatttctgt gacaatgagc ggacaagaag gttctgcgaa
    23821 aatctctgct ttgatggaaa aattccgtaa agaagcaccg acagaatttg ccggagcaaa
    23881 agttattcaa acagaagact ttaaagaatt gacgagaaca gaccgtgaag gaaacgtaga
    23941 aaaactaaca acaccgccat ccgacgtatt gaagtataca ttggaagaaa atggctggat
    24001 tgctatccgc ccttcaggaa ctgagccgaa aatcaagttt tatattggtg taaaaggaag
    24061 ttctacagaa gacgcagccg aaaaagtagc ctcttatgaa gcgattatca aaaagatgac
    24121 agaagaataa aaaacttgag actgatggaa aaagtggtaa actttagaag tcagtctttt
    24181 ttcaagttgg aggtgaatgg attgccagac acgcaaaaat caaaagtaga gaaagtcagt
    24241 caattattca aaatgttgag tgatcccact cgtcttaaaa ttctgctata tctgaaagac
    24301 ggggaacaaa atgtgacagc aatcacccaa gcagtggaaa tggagcagtc tgctgtatcg
    24361 catcaactac gtttattaag agaaaatcat gtcgtcaagt ctcatcgcga gggaaaagcg
    24421 attttgtata gtcttgatga ttctcatgta ctggatattt tgaaccagac actcaaacat
    24481 gtggaacatc aataacgata ggtgcaaata gataaacact ggagtgtgac aacgaagaaa
    24541 tttcgttgtc acactccagt gttttttaag aaaaagacaa aaatgatcaa tcgctttttt
    24601 ctgttgtttc agttccataa taactatgat cttctaggtc taaagcattc aaaataagcg
    24661 aagccgtttc ttcaatagat aaagatgcta cattgatcac tacacatcct agtttttcat
    24721 aaagatcatt cgcaaaatcg agttcagctc taattttttc gatatcggaa taagcagtat
    24781 cagggtttaa tccgtaagag cgcatccgtt ctttacggat attatttaaa atatctgggt
    24841 tatttgttag tcccacgatt tttttaggat cgatctccca aagctgtttg ggaatatgtg
    24901 cttgtgggat caaaggtaaa ttggcaactt ttaaattttt gttagctaaa aacaagctca
    24961 aaggcgtttt ggaagttcgg gatacgccta gcaaaacgac atctgcttct aaaaaaccac
    25021 gaggatcttt cccatcatcg tattttacag caaattccat tgctttgatc cgtttgaaat
    25081 agttctcatt caataaatgg agggcaccag gttgccgaat tggcttgata ccagttaaac
    25141 gttcaatttc tgatatcggt ggcgtcatca catcaaaact aaataattga tgttcttcac
    25201 aaaaagaacg tgttatctcg atcagttctt gattgattaa tgtatgcagg acaagtgctt
    25261 tttcttctaa tgcatcttgc aatgcctgcc ttaatgtttt ttcatctgtc acgaacgttc
    25321 tgcggaatag gttgaactca acggtaggat actgagccat agtagcttgc gctagttttg
    25381 tcgcagtttc tccagcagag tcggagatga caaaaaaagt aacgatttct gtagaatcgg
    25441 aagtcatgga taaaacctca cttgcatttt ttttatgttt attataccat actctagaat
    25501 gcgtacttcg taatcatttt gatttggagg gataagtcat gaaaaaacag acattgatcg
    25561 aacaatcatt agaaacattg aaaaataacg gttttaaata tactaaaaaa agagaagcat
    25621 tgttaactta tttagtgaag cgtaaccgct acgtatctgc taaagaagtt tttgactata
    25681 tgaatcagga attcaaaggg gtaagttatg atacgattta ccggaatctc catgattttg
    25741 cagagatcga tttactggaa gaaaccgatt taaatggaga aatgaagttt cgttttcatt
    25801 gctgtcaggg gaaaatcggc catcaccatc attttatctg tacagtgtgc ggaacaacga
    25861 aagaaatcca tatgtgtccg atgaattatt tcgaagaaca gctaggtgga tgcacgatcg
    25921 aagggcatag attcgagatt ttcggaagat gtgaaaattg tcaataagga tttaaataaa
    25981 ataacatttg ataattttaa aaaaaagcaa gatttccttg acggtcaaaa cagcattaga
    26041 tataatattt acaggacagt cttgtttttt gagatatatt tcaataacaa ttacggttac
    26101 tttttaagct cggagggagg gaattaacat gtcaaaaaca gtcgttcgca aaaacgaatc
    26161 acttgacgat gctcttcgtc gcttcaaacg ttccgtgtca aaagcgggta ctttacaaga
    26221 atctcgtaaa cgtgaattct acgaaaaacc aagtgtaaga cgcaagaaaa aatctgaagc
    26281 agctagaaaa cgcaaaaaat tctagttctg tgatgaattg gagttggtag tgtgtcacta
    26341 ctaagtagat taaacgatga catgaaagca gcgatgaaag cgaaagataa ggaatcttta
    26401 caagtcatcc gaatgatcaa gtcatctata caaaacgagc agattaaaga aggtcacgac
    26461 ttgacagaag aagaagaact aacggtgttg tctcgtgaaa tgaaacagcg acgtgattct
    26521 ttgcatgagt ttgaagaagc aggacgtgac gatttagctg aaaaagtaaa aagtgaaatt
    26581 gtaatagtcg aaaaatatat gccggaacaa ctttccgacg aagaaattcg acaacttgtt
    26641 caagaagcaa tcactcaaac tggtgcttca tcaatgaaag aatttggcaa agttatgggt
    26701 gcaattatgc ctaaagtcaa aggaaaagca gatggaaacc aagtgaatgc gattgtgaaa
    26761 gaactattac aatcttaatt ttatcattta aactgcggac agtgattatc cgcagttttt
    26821 tgtgttcgta aaaaaagaac taaaaaacag ctagaagaaa gaatcttatt
//
LOCUS       DACDRK010000031        26371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDRK010000031 DACDRK010000000
VERSION     DACDRK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.031"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            367..555
                     /locus_tag="IWJ77_001434"
     CDS             367..555
                     /locus_tag="IWJ77_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453044.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="IWJ77_001435"
     CDS             545..805
                     /locus_tag="IWJ77_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453045.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="IWJ77_001436"
     CDS             846..1073
                     /locus_tag="IWJ77_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453046.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="IWJ77_001437"
     CDS             1138..1710
                     /locus_tag="IWJ77_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453047.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="IWJ77_001438"
     CDS             1711..2022
                     /locus_tag="IWJ77_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9453048.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="IWJ77_001439"
     CDS             2013..2273
                     /locus_tag="IWJ77_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453049.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="IWJ77_001440"
     CDS             2434..2793
                     /locus_tag="IWJ77_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453050.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="IWJ77_001441"
     CDS             2790..4433
                     /locus_tag="IWJ77_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9453051.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="IWJ77_001442"
     CDS             4468..5550
                     /locus_tag="IWJ77_001442"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453052.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="IWJ77_001443"
     CDS             5594..6766
                     /locus_tag="IWJ77_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9453053.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="IWJ77_001444"
     CDS             6753..7457
                     /locus_tag="IWJ77_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9453054.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="IWJ77_001445"
     CDS             7462..8622
                     /locus_tag="IWJ77_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9453055.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="IWJ77_001446"
     CDS             8698..8976
                     /locus_tag="IWJ77_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9453056.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="IWJ77_001447"
     CDS             8957..9328
                     /locus_tag="IWJ77_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9453057.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="IWJ77_001448"
     CDS             9344..9748
                     /locus_tag="IWJ77_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453058.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="IWJ77_001449"
     CDS             9745..10155
                     /locus_tag="IWJ77_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453059.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="IWJ77_001450"
     CDS             10216..10803
                     /locus_tag="IWJ77_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9453060.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="IWJ77_001451"
     CDS             10876..11343
                     /locus_tag="IWJ77_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP9453061.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="IWJ77_001452"
     CDS             11389..11697
                     /locus_tag="IWJ77_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453062.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="IWJ77_001453"
     CDS             11892..18626
                     /locus_tag="IWJ77_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9453063.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="IWJ77_001454"
     CDS             18630..19502
                     /locus_tag="IWJ77_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453064.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20126
                     /locus_tag="IWJ77_001455"
                     /pseudo
     CDS             19499..20126
                     /locus_tag="IWJ77_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20087..20548
                     /locus_tag="IWJ77_001456"
     CDS             20087..20548
                     /locus_tag="IWJ77_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453065.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="IWJ77_001457"
     CDS             20552..21376
                     /locus_tag="IWJ77_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453066.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..23436
                     /locus_tag="IWJ77_001458"
     CDS             21394..23436
                     /locus_tag="IWJ77_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP9453067.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSIDFGGYD
                     YSGKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTY
                     TLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVN
                     YQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLG
                     ENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNF
                     GELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQIDWLKIEKGDTRTP
                     NISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSIEGLKNFEDGIQS
                     KGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGNDSTILT
                     IPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            23596..24042
                     /locus_tag="IWJ77_001459"
     CDS             23596..24042
                     /locus_tag="IWJ77_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453068.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            24044..24181
                     /locus_tag="IWJ77_001460"
     CDS             24044..24181
                     /locus_tag="IWJ77_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9453069.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            24218..24511
                     /locus_tag="IWJ77_001461"
     CDS             24218..24511
                     /locus_tag="IWJ77_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453070.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            24508..24732
                     /locus_tag="IWJ77_001462"
     CDS             24508..24732
                     /locus_tag="IWJ77_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9453071.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            24729..25748
                     /locus_tag="IWJ77_001463"
     CDS             24729..25748
                     /locus_tag="IWJ77_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9453072.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            25937..26010
                     /locus_tag="IWJ77_001464"
     tRNA            25937..26010
                     /locus_tag="IWJ77_001464"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25971..25973,aa:Ile,seq:cat)
BASE COUNT         9379 a         4046 c         5563 g         7383 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg atttcggggg atatgattat agcggtaagc cgaatttaat gtccaaacta
    22081 aaatcgagcg attttaacgt tggttaccac gggtcactaa cttcggataa cgaaaagcta
    22141 cattttactt ctgatggtac aggaagcatt attatgttta cgcgtattaa tacacctcag
    22201 cttgctagtg ggaaaaccta tactctgagt gcgaaagttc gatttgatga aggaactaca
    22261 ggagctattg ataaattacg tttggtgtat cgtacatcac caggagaaaa gatattattg
    22321 gaagcaaata gtacaaatat tacaacagat gatgtaggga aagaaataac aatcaaaggt
    22381 acagctaacg ttaattatca aatcacaaat ttagatcgat tttatatgag tattagcttt
    22441 gttgacaggg ataaaataaa tggcggattt aagttgtacg acatcaaaat cgaagaaggc
    22501 tcaacagcca caccgtacca gcctaactta ctagatgacc cttactgggt aggtaaaaca
    22561 tcgttgggtg agaatattgc aaataaaggt acgacgttcc caattaaatc tagcgcgtac
    22621 gaaatatata aaggtaacac ggaagaagag cttataatag gtcaaacgta tactatcacg
    22681 cttaaaggaa caaaacccgc aagtcaaacc tttgtagcat ataattattg gaacgttaat
    22741 tttggagaac taaaaccagt tgagggattg acagacgtat ggtctctaac attcacacca
    22801 acgaaacttg aaccgggttt gcctaaagac cttcgcattt ttcagttgcc taaagaaacg
    22861 gccggcgcat gccaaattga ctggctcaaa attgaaaaag gcgacacccg aaccccgaat
    22921 attagtcagt ttaaatactt tggtgaagga ttgaaagata gcaacaatcc gaacgactac
    22981 agctgggaca tcacgcctga atatactgaa aaaggcttga atgatacggt tagtttgact
    23041 gaaccgcaat ccatcgaagg attgaaaaat ttcgaagatg gtattcaatc aaaaggaaaa
    23101 tctgtattga catcagacga caacaaatat gaagtcgtaa ccttaacagt tacaaacggg
    23161 aataccggat cagcaaagct ttatcgtgaa ggaaaaaccg tcactattta tttttttgcg
    23221 ttgaatggga aaagcagtgg tggaaatgat tcaacgatac taacaattcc agaaggctat
    23281 cggccaccaa ttagttttga gcaactggtt ggctcgatag accgttctac tttgaacagt
    23341 gctcagttat ctattggtgc agatggagcc attaaatggc gaagaaactc aagttatgga
    23401 tcagcttatt catttgttat cacttattca atttaaggga ggaaatctaa tgaaagtagt
    23461 ttacaaatca atcaagcctt acggattcga gcaaatcatt gtgaatgacg aagaacattt
    23521 accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga aattcaatta
    23581 ctgggaggga aataaatgaa aaacatttgg aaatatggcc gtactggcgg agagtatgca
    23641 ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc accgctcgaa
    23701 ggggttcgtt cagatggcga accgctaacg attgctgatc agatgtttga tcctaaattg
    23761 aaccaatgga ttattttagc gaacgcgtta gatcacaacg atttaaacaa tctcaaagcg
    23821 atgtacgagg ctcttgaaca tgaaaacggc aacctaaaac agctaaatgc caaactcatg
    23881 ctaagcgatg tagcgattaa acaggaaaat actgcattga aagaaaaagc tgacagttta
    23941 gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga catttcagaa
    24001 attaaagagc aactaaatcc agcttcaaaa ggaggtgagt agtatgttta gttttagcga
    24061 tgtgaaaatg atgtatgatt ggggctgttt tactgacgat caagttcgac tattcgttcc
    24121 actatgcatt acagacgaag aagcagataa aatcattaat aaagataaga gcgcatctta
    24181 attgatgcgt ttttatttaa ggtaaaggag ttgtcacatg attaatttag gggaatgggg
    24241 aatgatagca ggatcaataa ccgctatcgt ttctttgatt ttattagtaa ttagaccgat
    24301 tgctgcatct ttctcgaaaa ttactgagac tctttcaaaa gtaagccaca atttagattt
    24361 gctgactaaa gatttagaat cgagcaaatc agatcgattg atgattcatg aagaactaaa
    24421 gaaacacgat gaaagattag atacacatac agaaaaattg gtggaacaca cgcaacaaat
    24481 caaaactttg tttagggaga gaagaaaatg aataataaaa cgttcgaagt actaaaatgg
    24541 ttcgcactgg taattattcc cgcactagct actttcgtgg ggttagttgg taaagcgctc
    24601 aattggcagt acacagatat ctgtgttgtc atcattactg gttttggcgc gtttttaggg
    24661 agtgtgttgg gtgtatcaaa tcgaacctac aaaatgttct cggctgaaag cgaagaagga
    24721 ggaaacaaat gaaaaagaaa attactatta ctgcgatgag cctgttaacg gctctttttt
    24781 tattgccaat taacggattt gcctatacta tcaacaatga atttaatttg ggcgcaaatg
    24841 aaggtagctc acaagtagca aataatcagt atattttact gcatgaaacg gctaatgaaa
    24901 cagcaacagg acgcaatgaa gcgcagtata tgcaacgttc atggactagc gcttatactg
    24961 cttatattgt gggagacggc ggaattgttt accaagtcgg acaacctggt tatgtacagt
    25021 acggtgctgg ttcatatgct aatgccaata gtcctgtgca gattgagtta caacacacac
    25081 atgataaagc aacttttgaa aaaaactata aagcatacgt tgaattggct agagattcag
    25141 caatgaaata tggtattcca ttaacattag acacgcctta taaccaacca ggaatcaaat
    25201 cacatttatg ggtaactcaa aacatttggg gcgatcacac tgatccgtat ggatacttat
    25261 cagaaatggg tgtaagtaag gaaaaactgg cttacgattt agctcatgga tttacagacg
    25321 aaaacccaac gacttctgag aacaagcctg tcattgatcc aacccgagca ggtgcagcaa
    25381 atccaacttt atcagatgga acgaaccatt ctcacattga tcagtttgga gaaatcgaaa
    25441 atgcgaactt acacgtcgct ggatggcaca tcgctaacta taagtacgag tatattttca
    25501 ttatggacta caatactggg aaagaattag ctcgagtaag agctgatgga atttatagac
    25561 cggatgtaaa tcaagcttat aatacttctg gaaatgttgg ctatcatgta tcttttaaca
    25621 tgcgtaactt ccctaataag aaagtctatg tcatgatgcg ggcaacgaat gatccagagg
    25681 gaaacactaa aggcggtgcg caagatttcc atgacaaacg ttggtattta aatattccta
    25741 aacgataaaa atagctcctc gttgaggagc attacataac tatattgaca actataaaaa
    25801 tcattcgata aaatagtgat gttatcgcat atcttcacta tcacccataa tagtcacact
    25861 ccaagctatg cgataacagg tttgttgcca cacattctac tggttgattg tttatggctt
    25921 tatgtggcaa caaccagtac ccttagctca gttggtcaga gcagacggct cataaccgtc
    25981 cggtcgtagg ttcgagtcct acagggtaca ttaacgtagc catttgaatc gttctgtgtt
    26041 agaatttttt gaagagtatt atacaagcta aagcttttct tcattgccac tcaaatgagt
    26101 ggctttttta tgtatccttt tatggattaa tgaaaggatg tttcacatag ttatatttct
    26161 gtatatttga aaagttttac tttgattttt aaatagaaag acatttgggt tatattgtga
    26221 gataataata aagaagagtt taaagcgttc cccaaaaacc actgccccat aagtgtgtta
    26281 cgctttaaac tcttttatat ttgaagccat taaaaagcat accatatttt tgaaaaaaag
    26341 tgagaaaaaa ggcttataat tggagtggta g
//
LOCUS       DACDRK010000032        26164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDRK010000032 DACDRK010000000
VERSION     DACDRK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.032"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(66..1097)
                     /gene="queA"
                     /locus_tag="IWJ77_001465"
     CDS             complement(66..1097)
                     /gene="queA"
                     /locus_tag="IWJ77_001465"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9453073.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1290..2465
                     /locus_tag="IWJ77_001466"
     CDS             1290..2465
                     /locus_tag="IWJ77_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9453074.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2469..3110
                     /locus_tag="IWJ77_001467"
     CDS             2469..3110
                     /locus_tag="IWJ77_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9453075.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3107..4024
                     /locus_tag="IWJ77_001468"
     CDS             3107..4024
                     /locus_tag="IWJ77_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9453076.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4021..4692
                     /locus_tag="IWJ77_001469"
     CDS             4021..4692
                     /locus_tag="IWJ77_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9453077.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5233..5721)
                     /locus_tag="IWJ77_001470"
     CDS             complement(5233..5721)
                     /locus_tag="IWJ77_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9453078.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5922..6188)
                     /locus_tag="IWJ77_001471"
     CDS             complement(5922..6188)
                     /locus_tag="IWJ77_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9453079.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6188..6451)
                     /locus_tag="IWJ77_001472"
     CDS             complement(6188..6451)
                     /locus_tag="IWJ77_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9453080.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(6444..6716)
                     /locus_tag="IWJ77_001473"
     CDS             complement(6444..6716)
                     /locus_tag="IWJ77_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453081.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            6927..7112
                     /locus_tag="IWJ77_001474"
     CDS             6927..7112
                     /locus_tag="IWJ77_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9453082.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7254..9077)
                     /locus_tag="IWJ77_001475"
     CDS             complement(7254..9077)
                     /locus_tag="IWJ77_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9453083.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9675..12380)
                     /locus_tag="IWJ77_001476"
     CDS             complement(9675..12380)
                     /locus_tag="IWJ77_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9453084.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12686..13633)
                     /locus_tag="IWJ77_001477"
     CDS             complement(12686..13633)
                     /locus_tag="IWJ77_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9453085.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13823..14092
                     /gene="rpsN"
                     /locus_tag="IWJ77_001478"
     CDS             13823..14092
                     /gene="rpsN"
                     /locus_tag="IWJ77_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9453086.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14268..14630)
                     /locus_tag="IWJ77_001479"
     CDS             complement(14268..14630)
                     /locus_tag="IWJ77_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9453087.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14641..15762)
                     /locus_tag="IWJ77_001480"
     CDS             complement(14641..15762)
                     /locus_tag="IWJ77_001480"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9453088.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15778..16128)
                     /locus_tag="IWJ77_001481"
     CDS             complement(15778..16128)
                     /locus_tag="IWJ77_001481"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9453089.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16258..17769)
                     /locus_tag="IWJ77_001482"
     CDS             complement(16258..17769)
                     /locus_tag="IWJ77_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9453090.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18091..19443)
                     /locus_tag="IWJ77_001483"
     CDS             complement(18091..19443)
                     /locus_tag="IWJ77_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9453091.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19596..20672)
                     /locus_tag="IWJ77_001484"
     CDS             complement(19596..20672)
                     /locus_tag="IWJ77_001484"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9453092.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20868..22613
                     /locus_tag="IWJ77_001485"
     CDS             20868..22613
                     /locus_tag="IWJ77_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9453093.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22893..23330)
                     /locus_tag="IWJ77_001486"
     CDS             complement(22893..23330)
                     /locus_tag="IWJ77_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9453094.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23314..24687)
                     /locus_tag="IWJ77_001487"
     CDS             complement(23314..24687)
                     /locus_tag="IWJ77_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453095.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25009..25497)
                     /locus_tag="IWJ77_001488"
     CDS             complement(25009..25497)
                     /locus_tag="IWJ77_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453096.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25519..26121)
                     /locus_tag="IWJ77_001489"
     CDS             complement(25519..26121)
                     /locus_tag="IWJ77_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9453097.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7506 a         5560 c         4836 g         8262 t
ORIGIN      
        1 ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca
       61 taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc
      121 gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact
      181 aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag
      241 ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc
      301 gttccaatcg tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca
      361 tttttacgta cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta
      421 tgcatatggt gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca
      481 tgtagtgtca gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc
      541 gtaaaatgca atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt
      601 tgatagcgat ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc
      661 aatgattcaa gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg
      721 tgttccaatt cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc
      781 ccaactttgg cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc
      841 gttagaagaa ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca
      901 ggcagaactc gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt
      961 tcatggaaat gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa
     1021 tgatcgcggt cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa
     1081 tcttctgttg ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg
     1141 tttttcgttc tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata
     1201 aaaaaactac cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct
     1261 tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt
     1321 ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca
     1381 tttgttttat cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa
     1441 tgactgatcc aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc
     1501 cagtggaatt gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca
     1561 tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac
     1621 ggaataaaac agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc
     1681 gctacccaca tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg
     1741 cagcgaatca agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc
     1801 gtgattcctt gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat
     1861 ttgtaaccca tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg
     1921 aaggccgtgt gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt
     1981 tcgtagaaga gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag
     2041 tcggcgaagt tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag
     2101 caatcaaact gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg
     2161 tattaaaagg atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg
     2221 tcggcgacat tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata
     2281 ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca
     2341 aagtcgtagg tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg
     2401 gagaagatcc cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg
     2461 tctgacctat gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg
     2521 aacatcttta tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg
     2581 gtgtcttact gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct
     2641 tgcagacaat cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta
     2701 aattacctgc gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt
     2761 acattgggat gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga
     2821 ctgagttcca atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg
     2881 gtattcgttt agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg
     2941 ctggtggttt aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt
     3001 tggcaggtac gatccctgta acaattttag cactattggc tgattggctt ctaggtctct
     3061 tggaacaccg gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact
     3121 aaaaatgacc atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct
     3181 tgccagcaat acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca
     3241 gatcttagca agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat
     3301 catcaacaat ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc
     3361 aatttcagcg gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga
     3421 aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca
     3481 aacgtggttc ccgtcctata gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac
     3541 cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt
     3601 agctggtgta gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga
     3661 aacttatggc ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc
     3721 agtcgaagca ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag
     3781 ttatgatcta gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc
     3841 agttgtagac aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt
     3901 aggtcatacc atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct
     3961 agtggaacca tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa
     4021 gtgaagcaat tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat
     4081 ggctcctata tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc
     4141 tttgcagcag ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca
     4201 aactgggtga ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc
     4261 attttaatca ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat
     4321 tccctcttac cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc
     4381 cttgatgtcg gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta
     4441 ccactctctg tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg
     4501 ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga
     4561 accaatgcaa cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg
     4621 gcgatcatca cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt
     4681 acatcacgct gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa
     4741 ggctttttta gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg
     4801 ggtaaagagt tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc
     4861 aacgggttca tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc
     4921 caatccattt tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc
     4981 tgcttgttct ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc
     5041 cttttccatc agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg
     5101 ccgtatcggt aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg
     5161 ctaaaaacat atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc
     5221 ggtacacgat tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt
     5281 caccagaaga gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt
     5341 ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga
     5401 aagcgccacg actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt
     5461 ggatacccgc tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc
     5521 aaaagaccat gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat
     5581 tgcttttttc aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc
     5641 ctcactttgg cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat
     5701 tttactgatt ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc
     5761 cattcctaaa ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa
     5821 tcatgcaata aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga
     5881 gactctttgt taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag
     5941 gcagaataaa tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt
     6001 ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc
     6061 gtgggcatat atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa
     6121 ttcgtttgct tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag
     6181 ttactcatca aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt
     6241 tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa
     6301 tcgctctcca gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa
     6361 tctcgatttc agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag
     6421 cagtgctagg attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta
     6481 tagcgtactt gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt
     6541 ttcctctttt ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca
     6601 cttcttactc ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca
     6661 tctttagcaa aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag
     6721 gttcctcgtt gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag
     6781 atttgtaacg gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg
     6841 attgcctctt tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg
     6901 ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac
     6961 ttgacgaggc tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg
     7021 aagaagctga aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc
     7081 aagacatcaa aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc
     7141 tgttcccatc tttaatggct tatagagggc acctttagct gagaataaga caaaacagac
     7201 cctctagctt caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc
     7261 ttcaaatggt agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt
     7321 aaccgcaaac ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga
     7381 ggaatcaaca cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca
     7441 taacggataa ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca
     7501 tctgggaaat gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct
     7561 agcgaaacat gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg
     7621 atatttaccc gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt
     7681 agttttgcat cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc
     7741 ttataaaaaa tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac
     7801 cgatagacaa acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca
     7861 atcgatttgc ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga
     7921 gataaagcaa atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag
     7981 ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa
     8041 tagcctagtc gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga
     8101 ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc
     8161 gctgtagcaa attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt
     8221 tgagacaaaa cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg
     8281 atccccacga agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt
     8341 tttgctcttg gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa
     8401 gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc
     8461 gctcctggaa gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca
     8521 acaacgatca gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat
     8581 gcagattccc tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa
     8641 atagcgactt gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct
     8701 gagacagata ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag
     8761 ccagcattct ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa
     8821 gcatggatga tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg
     8881 gcaatgggta gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt
     8941 tgttccgtac catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca
     9001 aatcgggtta atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac
     9061 ctttttaagt aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag
     9121 tcgaaaattt atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa
     9181 ctttaagaaa ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa
     9241 tacagcaaaa atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat
     9301 agaacttgac ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag
     9361 atgtaaggct gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa
     9421 cctcttgata aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa
     9481 aacataagaa gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta
     9541 caacctcttg gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc
     9601 aaaatatgag gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt
     9661 cacaacctct ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc
     9721 attttttctt cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa
     9781 ccgatcatga tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc
     9841 cgtaacaagt ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc
     9901 cctgagactt ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata
     9961 atagcgatcc ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg
    10021 aacgctaagg cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact
    10081 cctagtaaga aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa
    10141 aatgtcagca tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt
    10201 cctaaatagt attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat
    10261 agtgcagtat aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg
    10321 gtttgtagct caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat
    10381 aatttgatga cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct
    10441 ttttggatac cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc
    10501 acagctgttt cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca
    10561 ttttttagtg ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga
    10621 atgatcggta attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta
    10681 tccatatttc cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt
    10741 tctttttgat atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg
    10801 agaagcgaat caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc
    10861 gtatcgatga cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata
    10921 ccagcaatga tgccattgat attattaaca acggcatcaa tttgatctag caattcatca
    10981 atagccttcg tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga
    11041 ttgcttagca agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt
    11101 ccagcacttg tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt
    11161 tgagatattc cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca
    11221 gtcagcttac catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt
    11281 gccacttgtc caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca
    11341 tctttcagct gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct
    11401 aaatttttca catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt
    11461 gcttcatcga ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct
    11521 gctagttgct taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt
    11581 tttgcttgat ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc
    11641 tcatctactt tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt
    11701 tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa
    11761 ttttcatctg ttgataaaat catatcttta actttattga tacttactag gttcgtatcg
    11821 atgtcgtaac ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat
    11881 tcatttgtga tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc
    11941 gcattgattt tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta
    12001 aaactcagca gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg
    12061 gaacggactc cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta
    12121 tttttatgaa gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct
    12181 gctggcttgt ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc
    12241 cataatgctt tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc
    12301 agaagtgttg caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc
    12361 gtatttttga ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac
    12421 catgtatttc atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat
    12481 cacggcaatt gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct
    12541 ttactttatc atgaaataaa agggaaagat aattttatgc caaagttttt atctattata
    12601 cgtatacgca aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt
    12661 ttttcgtttt ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag
    12721 gaggatgtcc ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat
    12781 tcattgaaat aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg
    12841 cttctgcatc tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt
    12901 gccgcaaatc tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga
    12961 tctggatgcc ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct
    13021 gatcagcaaa ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta
    13081 atttttttac cttttcctca tttttactgg atgcactggt taagattcgg attccctttt
    13141 catggagaag ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag
    13201 tatctgtcgg ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg
    13261 tgaccattcc ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg
    13321 atggcaacac gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac
    13381 ctacatgaac agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac
    13441 ctggtacata agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa
    13501 catcataagg attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt
    13561 ctctgggatg agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg
    13621 caaatcttcg catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt
    13681 tgacaaaaaa aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt
    13741 tcgcgtaacg tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa
    13801 aaataattgg aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca
    13861 gcaagaaacg attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta
    13921 cgaaggcttg cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt
    13981 aatcgatgga cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg
    14041 tgaattagca cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc
    14101 tggctgtaat gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac
    14161 gttcgcttct tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca
    14221 gctggtcctt ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca
    14281 ggagtcagtg cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg
    14341 ggtcgatcca aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc
    14401 tccaacagga taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga
    14461 gtcggaagct taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta
    14521 aaatggttac ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat
    14581 cccacgactg gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc
    14641 ttattcttga tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat
    14701 cgtacctaat tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat
    14761 aagcgtcact tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat
    14821 acatacacgc ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat
    14881 ctttcgtggc cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg
    14941 agtgatatat gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat
    15001 caattcagaa accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc
    15061 agagggatta agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc
    15121 atgccataaa gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt
    15181 tttcagcact tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc
    15241 tgctgtagaa aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa
    15301 tgcagctgcc tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc
    15361 tgcttttaga tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc
    15421 tgcttgcaac cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt
    15481 cagtgcgtcg taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct
    15541 caactcgact ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc
    15601 tgcctcagct gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa
    15661 ttcttttcct tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt
    15721 atgtatcact gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta
    15781 gtcttccaaa ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac
    15841 attgccttca tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc
    15901 gacatcttga aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt
    15961 gcaagcgtaa cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag
    16021 tttcatttca tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg
    16081 agtgatccta ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct
    16141 tttcaataaa tttagaacac tctcattcta ccaaattata accattttgc cacaaataag
    16201 tccacaaata acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta
    16261 atcgttattt gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc
    16321 tttttggctg ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc
    16381 gccgtcacga cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt
    16441 tttctgcatt ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc
    16501 ttttgctgtc gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa
    16561 tttatctgct gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg
    16621 ttcaactgca gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt
    16681 cgtcatccgt tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt
    16741 aacgaaagtc acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac
    16801 gtagctttct ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc
    16861 taatccacgt gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga
    16921 acgcaatacg ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta
    16981 accgcgtgct tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac
    17041 gattgttaat tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta
    17101 ttcttttgcg cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat
    17161 cttcacatga tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat
    17221 cgttgctgag aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat
    17281 atcttccaag aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac
    17341 ggttccaagt ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac
    17401 aacgatatgt ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta
    17461 aacggcttgt acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg
    17521 gatagctaat tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc
    17581 gattttttct aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg
    17641 tccaataacg tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc
    17701 ttcttcaaaa ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt
    17761 aaatttcaaa tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc
    17821 cgtcgatttg ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta
    17881 tccattctta gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa
    17941 aagccaaacg ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt
    18001 ttctatggca aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata
    18061 gtttttcgtt ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac
    18121 ttcaaataag cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt
    18181 ttgtttgata gcatcaatca atttattctt ttctttttca aaatagaaca caggtagttg
    18241 ggggtatttt tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat
    18301 catggcgaat ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc
    18361 tttccctaat tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt
    18421 tttaaaagta tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa
    18481 aatagctgca ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg
    18541 ggcgagccct tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc
    18601 aatcaaagca ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc
    18661 tactacaaaa tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt
    18721 tccaataccg aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg
    18781 ttcatctgcg ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt
    18841 ggctttagcg attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt
    18901 gattgctgca gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc
    18961 catgcccatt tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg
    19021 aagaccaata ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa
    19081 gacagacgcg gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac
    19141 ttctgcgcct actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc
    19201 tgctacagga ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga
    19261 agcagatgct ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc
    19321 aagaggaacg aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat
    19381 ctttgtcagt gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt
    19441 cattgagatc tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg
    19501 catgactctc acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg
    19561 ttacgcaatc actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat
    19621 tgacgctgat ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat
    19681 ttcaagccca tattttccca taagagtggg tacatactga actccgtaaa tcctggcata
    19741 gagtttaatt cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc
    19801 aatccgcttc cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa
    19861 acttcttctg gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct
    19921 tcataatcat agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct
    19981 tcatttccta atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct
    20041 cgagaatcat actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct
    20101 tttgtaatgc cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa
    20161 gaaccttcac attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact
    20221 ggtacataag gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta
    20281 tccatggcac atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct
    20341 aagaagcctt ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg
    20401 gctccttctt ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc
    20461 tgtccacttg ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt
    20521 aatggacctt tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa
    20581 ttataataaa tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg
    20641 ctgcgtccgc catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt
    20701 tcagtgattt actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa
    20761 aaaaacgagc cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt
    20821 gtcccgtttt ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa
    20881 atccacgcag ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa
    20941 actttgtaca gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca
    21001 ccttcatcaa gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca
    21061 ctcaaacatt caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa
    21121 agacgatcga aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat
    21181 cgccttttgc ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg
    21241 cggtagtcgt taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac
    21301 tatttgaacg agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa
    21361 aaggggtact ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa
    21421 gtcaaaatgg cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca
    21481 agatttatga cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa
    21541 acttgaatca tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa
    21601 acaagcagct gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt
    21661 ttgatgatgg ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa
    21721 caaaagaaga acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg
    21781 gttcctttgc ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca
    21841 actacatcga ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac
    21901 aagccgtttt agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac
    21961 tttgtcagca agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc
    22021 ttgaacagta ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg
    22081 tcgtgacgat ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga
    22141 aagcatttgg gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg
    22201 aagtcgcttc gccttttgct caacattatt ttctcactca tctggatgta gaagatacat
    22261 tgaaacaaag cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac
    22321 agctccacca tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga
    22381 aaaagcaaga caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta
    22441 tgattttagc tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa
    22501 acgcagaggt cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta
    22561 tttttttggc aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg
    22621 ttgtgacaga aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca
    22681 aatttttgtg aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa
    22741 aaaatgtgaa aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa
    22801 cacatttatc ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt
    22861 cgttacctac tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga
    22921 cgaaaagaca tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct
    22981 tttcttaact ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca
    23041 aattcttttc cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa
    23101 ccataatctt gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc
    23161 gcactatcag ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc
    23221 gtataatggt ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt
    23281 atgacatctt cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc
    23341 cttcttccat aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca
    23401 ttttgccaag ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat
    23461 caataataga tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa
    23521 taaaaactgg tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg
    23581 atccattgga atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac
    23641 tacgtgccag aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac
    23701 caataaagaa gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt
    23761 caaaatccat ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat
    23821 aaaaatcgaa agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa
    23881 aaggaaattc cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt
    23941 ccattccttc ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc
    24001 ataaatagca agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa
    24061 caataagcca aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt
    24121 gatgtctttg aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac
    24181 gtaaaaaagt gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat
    24241 cgcttgatac aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt
    24301 gataaaacct tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc
    24361 tgcactcagc cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa
    24421 tattccaagc aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact
    24481 atctagacca aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa
    24541 cagcgtaaat ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag
    24601 ataaggaata aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg
    24661 cccaaatcgt ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat
    24721 ctgctcttcg attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt
    24781 aaaaaaaaat acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact
    24841 caagcagaca agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga
    24901 taaagataga aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga
    24961 gtccttctcg acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc
    25021 ttttttcatt ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc
    25081 tgtaatctca aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac
    25141 tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac
    25201 tgaagaagtc aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat
    25261 ttttccattt atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa
    25321 aaagtaggac acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt
    25381 cagcatgtaa tccacttgtt tttgaccaat gatcggcaca aaaacttccg gccatacttc
    25441 ttgtatgact gccatcagct catccagttc attttctgtt acttttattt tttccataca
    25501 cccacgtcct tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat
    25561 catgctatac acacccaccg accacggttt caagaaaatc acaatctgca taaattccct
    25621 aaaagataat ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag
    25681 cagcaaggta acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc
    25741 aaatttctga tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg
    25801 gacaagcaaa aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc
    25861 aaatccattg ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg
    25921 aatgatcact tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg
    25981 ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag
    26041 aatcaacaca aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa
    26101 aactgatcct tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc
    26161 tcct
//
LOCUS       DACDRK010000033        26163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDRK010000033 DACDRK010000000
VERSION     DACDRK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.033"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..3659
                     /locus_tag="IWJ77_001490"
     CDS             132..3659
                     /locus_tag="IWJ77_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9453098.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3656..7378
                     /gene="addA"
                     /locus_tag="IWJ77_001491"
     CDS             3656..7378
                     /gene="addA"
                     /locus_tag="IWJ77_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9453099.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7447..7788
                     /locus_tag="IWJ77_001492"
     CDS             7447..7788
                     /locus_tag="IWJ77_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9453100.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8097..9140
                     /gene="pheS"
                     /locus_tag="IWJ77_001493"
     CDS             8097..9140
                     /gene="pheS"
                     /locus_tag="IWJ77_001493"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9453101.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9145..11565
                     /locus_tag="IWJ77_001494"
     CDS             9145..11565
                     /locus_tag="IWJ77_001494"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9453102.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IWJ77_001495"
     CDS             complement(11735..12745)
                     /gene="trpS"
                     /locus_tag="IWJ77_001495"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9453103.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12999..13736
                     /locus_tag="IWJ77_001496"
     CDS             12999..13736
                     /locus_tag="IWJ77_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9453104.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13750..14565
                     /locus_tag="IWJ77_001497"
     CDS             13750..14565
                     /locus_tag="IWJ77_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9453105.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14578..15225
                     /locus_tag="IWJ77_001498"
     CDS             14578..15225
                     /locus_tag="IWJ77_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9453106.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15241..15897
                     /locus_tag="IWJ77_001499"
     CDS             15241..15897
                     /locus_tag="IWJ77_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9453107.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16059..16880
                     /gene="racE"
                     /locus_tag="IWJ77_001500"
     CDS             16059..16880
                     /gene="racE"
                     /locus_tag="IWJ77_001500"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9453108.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16883..18238
                     /gene="rph"
                     /locus_tag="IWJ77_001501"
     CDS             16883..18238
                     /gene="rph"
                     /locus_tag="IWJ77_001501"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9453109.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18239..18757
                     /locus_tag="IWJ77_001502"
     CDS             18239..18757
                     /locus_tag="IWJ77_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9453110.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18864..19364
                     /locus_tag="IWJ77_001503"
     CDS             18864..19364
                     /locus_tag="IWJ77_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9453111.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19644..20564)
                     /locus_tag="IWJ77_001504"
     CDS             complement(19644..20564)
                     /locus_tag="IWJ77_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9453112.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20729..21742
                     /locus_tag="IWJ77_001505"
     CDS             20729..21742
                     /locus_tag="IWJ77_001505"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9453113.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="IWJ77_001506"
     CDS             complement(21852..22607)
                     /gene="fabI"
                     /locus_tag="IWJ77_001506"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9453114.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22827..23267
                     /gene="fabZ"
                     /locus_tag="IWJ77_001507"
     CDS             22827..23267
                     /gene="fabZ"
                     /locus_tag="IWJ77_001507"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9453115.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23382..24143)
                     /locus_tag="IWJ77_001508"
     CDS             complement(23382..24143)
                     /locus_tag="IWJ77_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9453116.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24332..25426
                     /locus_tag="IWJ77_001509"
     CDS             24332..25426
                     /locus_tag="IWJ77_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9453117.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25512..>26163)
                     /locus_tag="IWJ77_001510"
     CDS             complement(25512..>26163)
                     /locus_tag="IWJ77_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9453118.1"
                     /translation="GMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIH
                     DRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETAL
                     NQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGIL
                     RRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8646 a         4535 c         5509 g         7473 t
ORIGIN      
        1 ttttgcgtat acttaaaaaa gtagaaatat cagaacctta aaaggatata atttgaagaa
       61 tatagaacaa atgttcagta ttttttgata taataaagga gcaccgtatc atgtgttgaa
      121 ggaggaaaag catgtcttta caatttattc ttggaaacgg gaaaaaagat caccgtcaag
      181 tcttgatcga cgaagcatcc gcttggctcg accaggatgc aagtaaccaa gtatttttcc
      241 ttgttcccaa ctacagcaaa ttcgaacagg aacaagaaat tttagcggag atgcgaagaa
      301 gaagcggaaa aaaaactttc agcacaacaa acgttcaagt atttagtttt tatcgtctgg
      361 cttggtattt gctgcaacag acaactcttc ttacaggaaa tgaattatca gaatcaggat
      421 ctgcaatgat cttgcgacag attttagaaa aaaatgagga agaattaaca atctttcgcg
      481 gagaaatcaa taaaagaggg tttattcgtc aattacaaga actctacagt gaattgcgta
      541 caggaaatat tcaaccagaa gacctgatgc ttttatttgg tgattcacct aatgcagaag
      601 accagcaact taaaatgaaa gacttgaagc tgattttttc agcttatgac agcgaattga
      661 ctcaacgtgc actaacaaat gaagatgcac tatctatact agcaaattat atgcagacac
      721 aagatttcaa caacgtcaaa tttattgtca gtggtttttc tcgaatcaat ggtcaagagt
      781 atcaattact gcaggcaatg atggaaaagg ggcaattggt tgtcgattta ttgctggatc
      841 gtccttatac agcggatctt ccagaagtac ttacgctctt tcatgagact ggaaagcttt
      901 atttccgtct ttttcaaagt gcaagagaac ggaaagtccc tatattagtg gaccgatttg
      961 caccagagca gactcgtccg acagagcttc aactgctgca aacaatgtgg gaagagacat
     1021 ctagccaaaa gaaagcggca gtacacaaaa tagggggaca agagcatctc catttgtggg
     1081 aagctgaaaa tccaactgaa gaagtccgac aattagctgt tgaaatcaga cgactagtca
     1141 gtgaagaaaa ctaccgttat cgtgatatcc aagtgctgac aaaaaatatg tctttatatg
     1201 gaaatatcct tcggccgatc tttcaagaaa tggcaattcc tttttatatc gatgaagaac
     1261 aaatgatgga aaatcatccg ctgatcgaat ggatcaacag tctgttcgct cttgaccgct
     1321 attcttatcg gctaagtgat attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg
     1381 aagcagaaac cgaacttgat gaatggaaac aagagcgaga tgaattcagg aaaaaactgg
     1441 acatcactga aaatgtagca cttgcttaca attatcaagg aacttattgg actcgtgaaa
     1501 aagactggca gtttgtggcg tatgattttg aagcagaaca gattgaagac gtacagtatt
     1561 tggaagaaca gtcaaatacg attagacgat tatttcagaa agctgtacca gtatttttga
     1621 aaaaaataaa gaaaagcaaa acaggacaag aggccgcaaa tgttttttat caatttttga
     1681 tccaaagtgg cgtacagcaa caattattgt tttggcgtaa tcaggaagta gacagaggaa
     1741 acttagaagc ggcaagaaac catgagcaga catggggagc gctgattgat ctgttggatg
     1801 aatatgtagt aatttatggt gaatcggctt ttgactggtc actgttccaa gaaatcatta
     1861 caagtggtct agaaaatcta agttatggta aaatccctac cgcaatcgac caagtgcgaa
     1921 tcaatcgttt ggaattagtt cgaccaaatc aagctgccgt cacttttgca ttagggttga
     1981 atgatcaagt gtttccagac cgaaaagaga caaaaggatt gttgtccagt gaagaacgag
     2041 aagtattaaa tcaggccttg ccagatggac agtttttatt cgacccatca aaggaaagta
     2101 tctcttttga gcctttccaa gcctatcttg tttttctttc cggaacagat cgattatatc
     2161 ttagttatgc acaaagctat gacacagatg gttcgctgaa gatgtctcct tatttgcgta
     2221 gaatcacaga atatttatct gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg
     2281 accctaattg ttatgtaggg acttacagaa gcggaatcaa tacagtcaat aggatttatc
     2341 gattagcttt agatgaaaaa aaacctgtac caaagcactg gcaaaagctc aaagaattag
     2401 tactgaattc gtcatggcga aattttgctt tacagatttt tgaaagtcag gaacatttaa
     2461 atgttcctgt ttcgatgcca gaaaaaatgg cggaagagct ttatggaaaa gagatttata
     2521 cttcaatttc acgtatggag aatttttata attgtgaata caaatacttc atgcaatttg
     2581 gtttgaaatt gaaagaacga actgtttatg gtctaactcc agcagcaact ggtgattttt
     2641 atcatgaatc attggatcgt ttcttcaagc tactgttttc aaaccaatta tccttagtga
     2701 atatgtccga tgcgcagcgt agagacttta ctgaaaaagt gctgcaggaa gtattcggag
     2761 aacttcggtt tgagatattg gatagttctg caagaatgaa ctatatccgc taccagttag
     2821 gacagacgat tcaaaaagtg acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc
     2881 atcctcttca gacagagatt ctatttggtc agattgctgg agcaaaaggt atcacaggtc
     2941 tggaattacc gttgagtaat ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg
     3001 atgtagcttc tgaacaagaa gatacttggc taagcgtcgt tgattacaaa tctagcggac
     3061 gttcattcga tgtcacagaa gcttattatg gcatggctat gcaattattg acgtatctgg
     3121 acgtagcatt gatggatgcg gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat
     3181 catattattt acatgttcat aatcctgtat tgacggagac agatgatgta gagaagaata
     3241 cattgaaaaa atttagttat gatggcgttt ttgtcgatga tcccgctctt ttagaagtac
     3301 tagatcattc actggaagca aaagaacaat cactgatcta tccagtgaaa aaaaatgcga
     3361 aagatgaata ccaaaaaggt agtttcagta aagacaaatt tttcacggaa gaagaactgc
     3421 aactattcat ggcgcataat cgggaaaata tgcggttagc tggagatgcg attacttccg
     3481 gagaaatcaa actaaatcca gcttataaag ataaacagcg gattgcctgc gaattttgtc
     3541 cattcagaag catctgtacg tttgatgtga tgctgaaaga aaataattac caccgtctgg
     3601 agaaaataga taaggaagaa gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa
     3661 tcaaattcct ttaaaaccgc aaaatgaacg attcacagat gatcagtggc aagcgatttt
     3721 tgatcaagga gataatctat tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct
     3781 tgttcgaaga gtgattgaaa aactaaaaat gggttttgat atcgatgaat tattgattgt
     3841 gacatttact gaagctgccg caagagaaat gaaagaaaga attcaagagg cattacaaga
     3901 atccgtcaat agcgaaagtg accctgtacg tagacagcat tttactaagc agttagtttt
     3961 attgcccaca gcgaatatct caaccttaca tgctttttgc ctgacagtta ttcgacgtta
     4021 ttattactta attgatattg atccagtctt tcgcatgtta acagatgaaa cagaaacgat
     4081 cttgatgaag gaggatgtgt gggacgagct gagagaagca ctttatgcag aaaatgatga
     4141 acgatttttc caattaacaa tgaatttttc taatgatcgt tcagatgacg gcttgacgaa
     4201 tctggtgttt tctctttatg agtttgcccg tgccaaccct gacccccaaa aatggctaga
     4261 acagttaagc gataattatc gtttgcctga ggggctagcg aaatctcgat tatatcaaaa
     4321 acaaatccgt cctttagttt tggcagatat ataccaatgt gtacagttat atgaacagat
     4381 gactcagtta gcacaaggtg aaggattaga aaaaatgcat gagcaagtag ctggagaaca
     4441 acagcagatc aagaatatct atgaagcgtt ctcgcaagat cggttagaag aagcttatgc
     4501 tggtttggaa caactaactt tttcaacttt taagagcagt cggaaagcag aattgaaaga
     4561 aatttccaat gaagtcaagg gcatgaggga taaagcaaaa aaactgattc agcagataag
     4621 taaaagttat tttcctgttt ccccaagcca aatggaagaa ttaacggaca aagcactacc
     4681 gttagtcgaa gaaatgacaa aagtcacgca aagcttcatg gacggtttca gtatgcgtaa
     4741 acgggaaaaa ggtgtgctgg atttcaatga tctggaacat ttagcgttgc agattctcac
     4801 agaaaaaacg aaagatgtat ggctgccaag tgaagcttca aaacattatc gaaaaaaatt
     4861 caaagaagta atggtagatg aatatcaaga cgtgaaccaa ttacaagaag ccattttata
     4921 ctggctaaga gaacccgatg atacaaaagg aaatatgttc atggttgggg atgtcaaaca
     4981 atccatctat tctttccgtt tagcagatcc tagtttattt atcgggaaat acgagaattt
     5041 ctcaaagaaa gaaggcggac gtcgaatcgt tttggctgag aacttccgct ctagaaaaga
     5101 agtcctttct tttacgaatc ttatttttga acaactaatg gatccagcag tgggtcaaat
     5161 caattatgac gaagcagcca aactgatcca aggattttca gactttccag agaatgaaca
     5221 gtttgagccg gaaatcatga tctatgaaaa agaacaggaa gaatcagaga ttgagattcc
     5281 gacggatgat atactggaag acaaaacaga aggcgaactt tttatgacag gactaaaaat
     5341 tcgtcaatta attgattcct cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc
     5401 gatagagtac aaagacattg ttctcttgac acctacaaag aagaacaact taacgatttt
     5461 agagattttc aaaacattgg atattccatt agagatgaat gatgctcaaa actattttca
     5521 agcgacagaa attcgcacga tgatctcgct gctacaatta atcgataatc cctatcaaga
     5581 tattccgctt gcggctgtat tgcgctcacc gatcgtgggt ctgattgaac cagaattagc
     5641 cagcattcga ttagctaacc gtgcacatac ttactatgat gctgtgcttg cttatcaagc
     5701 atcaaacgaa gatgaattag cagctaagct ggaacatttt ggcaagcaac tagagcattg
     5761 gcgagaactt gctcgccgtt cttcgatcac tgacttgtta tgggatatct attatgagac
     5821 gggatatcta gagtatgtgg tcggtctgcc agcaggggca cagagacaag ccaatcttta
     5881 tgcattggtt gatcgtgcca aagcttacga gcaaagttct ttcagaggat tataccagtt
     5941 cgtgcgtttc attgaaaaaa tgcaagaaaa agataaagac ttagcagaac cagtcatttc
     6001 aatagaagat aatgctgttc gcgttatgac gatccatgca agtaaaggct tggaattccc
     6061 agttgtattt ttgttagata tgacaaagga gttcaactta caagacttgc gcaatcggta
     6121 tgcttttgaa gaaaaacttg gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt
     6181 atatgatacg ttaccgtttc aagccatcaa attggcaaag caaaacaaat tgttgtcgga
     6241 agaaatgcgt aaattgtatg taggattgac tcgggcagaa caaaagcttt tcattgtcgg
     6301 ttcctataaa aataaagagc aaatgatcca aacgtggtct gaagcagctg atcatgagga
     6361 gttagttttt gatcctgcgc tacgtttgaa gggcagaagc agcttgatga attggattgg
     6421 ttacggattg atccgtcatc ctgaaatgca gaaatactta gaggaagaaa tttcgacttc
     6481 tcttcttcaa cattcaaatg cacagttttc tatttcatgg atgaatcagc aatcgatcat
     6541 tgaacaaaga cagttgcttg ctgaaaaaga actggttaac ttagatcagc agatgaaaga
     6601 agatgaaact ttattagcag attcgttaca aaaacgtctg gcatatgaat atccttatca
     6661 agcttcttca caaaccacaa gttatcaatc tgtatctgaa atcaaacgat tattcgaaga
     6721 tccagatgat acacaagaat cacgtttgac tctggaaagc agtcagaaca aagcagcatc
     6781 ccgtcaattt cgttatacgc aagaacaact agcagaacca aaatttttgc aaaaagatcg
     6841 gcaagtgtca gcagctgctg ttggaactgc tacgcatgct ttgttacaat tactgccatt
     6901 agagatgcca actacagaga gtatccatca aaaattacag gaattggtaa ggaaacggtt
     6961 agtagacgaa aaagtcgcga aaaaagtaga tgtttcttct attatatggt tctttcaaac
     7021 agaacttggt caacagctga ttgccaacaa agaaaacgta aaacgagaac aacctttttc
     7081 catgctgctt cctgctgacg aagtatttca agactatcca aatcaagaag atgaactatt
     7141 aatccacgga atcgtcgatg gatacctaga agaaaaagat catctcaata tctatgattt
     7201 taaaacagat tttattttac atccggatga tccagcagaa atagatgcaa tcgtccaaaa
     7261 atatcaagga cagctaagac tttaccaaca agcaatgtca gaagctttga ataagccagt
     7321 agaaaatgtt ttcctaattt tgcttcgagt gaaacaaatt ataaacatta acaaataatt
     7381 ataattgagc aaacttgact tatgttttgt aaggattatg ctatactctg ggaagaggtg
     7441 agtatgatgg agctaacaca gacagaattc gttttatgcc ctaaatttga aaaaacattc
     7501 tctattttag gaaaaaaatg gaacggattg attatcgatg tactactcga aaacggtccg
     7561 caacgctttg ttgagcttgc ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa
     7621 cgcttgaagg aattagaaaa agaaggaata gtcactcgaa actttgcctg tagcgaaaag
     7681 agccgttcag aatatctctt gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa
     7741 atacaagttt ggagcgaaaa atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa
     7801 aatcttgtaa actatagtcc atacgaataa gtaaagaacg atgacgaaaa agagtagttt
     7861 ggctaaacca gttcagggag atctgtcgca gactgaaagc agatcctgtg tttgaaaaat
     7921 gaagttcact ttccgagttc gtctgataaa atcagaccgg caattgtcgt taacgaagta
     7981 agagtgcggt gtaagtcatt tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct
     8041 taacaggaat gaggtctttt tgttttgtat ttggaattta aaggaggaac aagcacatgt
     8101 ctctacaagc acaattagag agcttgaaag aagcaacgct cgtcaaaatc acagcggcac
     8161 aagatttaaa tgaattaaat caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca
     8221 cagaagtatt acgtgggatg aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt
     8281 tcgcaaatga gatccgagat cttttgacag aagagatcga gcgaaaaaaa caagtattag
     8341 aagaagaagc gttaaatgca gctttggcaa aagaaacaat agacgtgaca ctacctggta
     8401 gaaaagtaaa acatggtaca cgtcatatcg tgacacaaat catggaagaa attgaagata
     8461 tttttgtggg tatgggctat cagatcgtag aaggatatga agtagaatct gatcattaca
     8521 atttcgaacg gatgaatctg ccaaaagatc atccagcaag ggacatgcaa gatactttct
     8581 atatttcaga cgagatattg attcggacac atacttcacc agtccaagca cggacaatgg
     8641 aaaaacatga tttctctaag ggtgctctac gaatgatctc gccagggaaa gtcttccgca
     8701 gagatacaga tgatgcgacc cacagtcatc agttccatca aattgaaggg ctagttgttg
     8761 ataaaaacat cacaatgggc gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt
     8821 ttggggaaga tcgtgaaatt cgtttgcgtc caagttattt cccatttacg gaaccatcag
     8881 tagaagtaga tgtcagctgt ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca
     8941 ctggatggat cgaaatttta ggagctggca tggtacaccc gaatgtgttg aagatgtcag
     9001 gaatcgatcc agaagaatat tcaggctttg ctttcggcct tggaccagac cgcgtagcta
     9061 tgttgcggta tggagtgaat gatatccgta atttctatca aaatgactta cgtttcttaa
     9121 gtcagttcaa gggggagtaa gaaaatgtta gtttcatata aatggctcaa tcgttatgta
     9181 gatttatcga atgtaacacc aaaagaatta gcggataaga tgtctgtgac aggaatcgaa
     9241 gtagagggag taacggttcc agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag
     9301 gaatgcgtac cacatccaga ttctgatcac ctttctattt gtcaagtcga tattggcgaa
     9361 gacgaactgt ctcaaatcgt ttgtggagcg cctaatgtga aggttggaat caaagtgatt
     9421 gttgcacttc ctaactcacg tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt
     9481 ggccaagtat caaatggcat gatttgctca ttacaagaaa ttggctattc agatagtgtt
     9541 ataccgaaag aatacgctga aggtatctat tatatgccac aagaagctgt aaatggcgat
     9601 ccagtttttc cttatttgga tatggatgat tcgattattg aattatctat cacaccgaat
     9661 cgtgcggatg cattgtcaat gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag
     9721 actcctacat tcgatgacga aaagctggta gaatcctcac gtcctgctag cgataaaatt
     9781 caagtggaag ttgaagataa agaagcagtt cctgcttatc aaatcagaat cattgaagga
     9841 gtaagaatcc agccaagtcc tcaatggctg caaaatcttt tgatgaacga aggcattcgt
     9901 ccaatcaaca atgtagtaga tgtgacgaac tatattttgc ttttattcgg acaaccacta
     9961 catgcatttg actacgacaa attgaaatca aaagagatct ttgtccgccg tgcaaaagaa
    10021 aatgaaacat tagtaacact agatggagaa tctcgggaat tgacaactga caatctcgtc
    10081 attactaacg gcaaagaacc agttgcttta gctggtgtca tgggagggtt agactcagag
    10141 atcacgaatg aaacaacaac agttgcacta gaatctgctt tatttgatcc aatttcgatt
    10201 cgtcgtacag caaaacaatt taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga
    10261 atcaaccatg cgactgttgg agaagccagt gaggttgctg cagcaatgat cgtttctctt
    10321 gcgggtgggg aagttctaca aggcgcggtc aaaggctcag agatcaaagc agaagacgta
    10381 tcagtgaaga tttcacttga acgcatcaat caatatttag gaacatctct tgtcgttaaa
    10441 gaagtaaatg agatttttga agcacttgga tttagctatg aagaaaagga gtatgtttat
    10501 tccgtagtag taccaccaag acgctgggat atccaaatcg aagcagatat tatcgaagaa
    10561 gttgctcgta tttatggata cgaccagttg ccatctacac ttccaagtgg agaaacggtt
    10621 gctggaagct tgtcaaaaga acaagaaact actcgtaaaa ttcgtacaat ctttgaagga
    10681 agcggattga gcgaagcaat cagttatgca ttgacaacag aagaaaaatc aagacagttt
    10741 gttataaatg aaagtaatat tacacgtttg gactggccaa tgagcgaaga acgttctgta
    10801 ttacgaatga atttgatcag cggactatta gatgatatca gttataacac tgcccgaaaa
    10861 aatacagaga ttgctttata tgaaattggt cgggtgttct tccaagaaaa tgatccacgt
    10921 acacatctgc cgcaagaagt aaaacatgca gcaatcgcta tttctggtat ttgggaagaa
    10981 aaagattggc agacgaaagc tcaaaatgta gactttttca ccatcaaggg tcttttagaa
    11041 aacttatttg aacaactagg catcactgaa gatgtccatt atcaaacagt gacagaaatg
    11101 aaggaaatgc atcctggaag aactgctgcg atttaccttg gcgaaaaatt tattggtttt
    11161 gtcggtcaag tgcatccaac gattacaaaa agttatgata ttccagaaac atatgttgct
    11221 gagtttgatt tatctgctat agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg
    11281 acaaaattcc cagcagtcag ccgggacatc gctttgcttg taaaagaaac tgtgacaaat
    11341 caggaattaa cagaagttat ccgttctgct gccggaagat ttttaacaga tatccaatta
    11401 tttgatgtct atcaaggaga aaacattgaa aaaggacata aatccatggc gtatagcctg
    11461 acattcgtca acccagaagc aacattgaca gatgaagaaa tcaataaagc aatggaaaaa
    11521 gtcgaaaaag cgttggtaga aaacctagag gcaagtattc gctaagaagg aaaatatagt
    11581 aagtaacgat caagtagctt ttgaagacca tacatttgaa aaaaagatgt gacacaactt
    11641 cgtcacatct ctttttttga ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca
    11701 ggcctttaca gcctgctttt ttagagtaaa tgaattaatg gaagtaattg attcccattg
    11761 ctgattttac ttcagaaagt gtttgtgcag ctactttagc agcttcttca ctacctttac
    11821 gtaagatttc cataacagct gaaggatctt tttccaattc ttctcggcgc gcacggatcg
    11881 gtgcaaattc agcttccaat acatcaatga ggtatcgttt gatctttaca tcaccaagtc
    11941 cgccgcggcg gtaatgagct ttcatttctt caattgcttc tttatctgtt ccgaatacat
    12001 ccaagtaggt gaagaccata ttcccttcta cttgtccagg atcttgtaca tggatgtggt
    12061 ttggatcagt atacatactc atgacttttt tctgtaagac atcggcagga tcggagatat
    12121 aaataccgtt tcctaacgat ttactcattt tgccatttcc atcgattcca ggtaatctac
    12181 ccattccttt tggtgggaat acgccttttg gttccaccaa gacttcacca tatgtatgat
    12241 tgaagctttg tacgatttcc tgtgtctgct ctagcattgg tttttgatcc tcaccaacag
    12301 gtacaagatt cgctttgaat gcagtgatat ctgctgcttg agaaacagga tagataaaga
    12361 atcctgtagg cacgctttca ccgaattttt tttgttcgat ttctgttttg acagtcggat
    12421 tgcgccgaac gcgtcctacg ctgactaagt tcaaataata catggttaat tcagctaatt
    12481 ccggaatttg tgattggata aataaagtag attttgcagg atctaatccc acggctaaat
    12541 agtctaaagc aacttgcaag acatttgatg aaaccttctc aggattttta gcattatctg
    12601 tcagtgcttg catgtcagca atcatgacaa acagttggtt gtcttcatct gcctgcatag
    12661 ccactcgggt ttttagagaa ccgacgtaat gccctaaatg cagcttccca gttggtcgat
    12721 ctcccgttaa aataatattt ttcaaaaaag acacctcttt acgatttgta agtctgagca
    12781 cttaatatct taccgttttt cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa
    12841 agaggataaa ataactaata gattagaaat ggaagaaata tttatgggag aataaacgtt
    12901 ctaagaaact ttacaaatta ctaaactatc agatataatg tattttatta tacatcttta
    12961 aaagaaatct tactaaataa taatgggagt gtaattctat ggcaatgatt aaatttgatc
    13021 acgtggataa atattatggc aagttccacg cattaaaaaa tatcaatctc gaattcgaga
    13081 aaggagaagt tgtcgttgtc attggtcctt ctggttcagg aaagagcacg atgcttcgct
    13141 gtatcaatgg attagagacg atctcttcag gtaagctgct gattaatgat actgacttgc
    13201 atgataaaaa gacaaaactg acagaagtcc gaaaaaacat cggcatggtt ttccaacatt
    13261 tcaatttgta cccaaataaa actgttctgg aaaacatcaa attagcacca atcaaagtgt
    13321 tgaagcaaga ccaggcaact gcagtcaaaa acgcggagaa atttttgaaa actgtcaata
    13381 tgttagataa gaaagattct tatccttcca tgttatccgg gggacaacag caacgggtgg
    13441 caatcgccag aggtttagcg atgaatcctg agatgctgct gtttgatgaa cctacttctg
    13501 ctttggatcc ggaaatgatt ggtgatgtat tagacgttat gaaaaaattg gctcatgacg
    13561 gtatgtcaat gattgtcgtg acacatgaaa tgggctttgc caaagaagtg gcggatcgtg
    13621 ttatcttcat ggctgatgga gaagtattgg aagacagcag aaatgtgaga gactttttcg
    13681 aaaatccaaa tgaagtccga gcacagcaat ttatcagtaa agttatcaat cattgatagg
    13741 aggaaaaaga tgcgcaggtt aaaaaaaata cgcctgattt tccttttagg cattgctttt
    13801 cttgcactag ccggatgtaa aggaaatggg ttggcaaatg aagatatttt agagcgaagc
    13861 aaatctacca atgaaatcat ctggggtgtc aagtatgata ctcgattatt tggaatgatg
    13921 gatattgaga gcagaactgt acaaggattt gatgtagata ttgcaaaagc aatcactaaa
    13981 aagatccttg gtgacaatgg aaaaacagaa tttgttgaag tcacttctaa aactcggatt
    14041 ccattattga aaaacggaaa tatcgatgca attattgcaa cgatgacgat cacagatgaa
    14101 cgaaaaaaac aggttgactt ttcagatgtc tatttcgatg ctggccaagc tttgttggta
    14161 aagaaaggaa gccaaatcaa aagtgtagat gatttgaatg cttctacaac ggttctagca
    14221 gtcaaaggtt ctacatcggc tgcgaatatc cgacagcatg caccagatgc aaaaatttta
    14281 gaattagaaa actatgcaga agcatttact gctttacaat ctggtcaggg agacgcgatg
    14341 actacggata atgcgatttt gttagggatt gcagatgaaa atccagagta tgagttagtt
    14401 ggtggaacgt ttactaatga gccttacggg attgcgatca acaaagggca agaaaacttc
    14461 ttgaaagctg ttaaccaagc gctagaggaa atgcatgcag atggcacata cgataaaatc
    14521 tatcaaaaat ggtttccaaa tgaaactgaa ggaaaagtag agtaaggggg agaggcaatg
    14581 agcgaattga tacaaactta tggaccaatc tttgtcgacg ggttcaagat cacattgttt
    14641 tcgagtgtct tagcacttgt gtttagtttg attatcggaa cgttaatggc aatcttccaa
    14701 ttatcaaaga ataaagtagt gagcgcctta gcaaaggcat atgtagaatt ttttagaaac
    14761 attccattat taatcatcgt gatgtttttt tatgttgtcg taccgattta ttggtttaat
    14821 ctagatggat tccaagcagg tacgattgga ttaacgatct atacatctgc ttttattgct
    14881 gaaacagtac gttcagggat tcaaaccgta ccaaaaggtc aaacagaagc aggactatct
    14941 tctggtttta cctattcaga aatcatgcgt tatattgttt taccgcaagc attcaaaatc
    15001 gttatcccgc cattaggcaa ccaatttatc aacctcgtaa aaaactcatc tattttagca
    15061 atcgtagctg gtttagattt gatgtatcaa ggagacttga tcgctagtga aacattcaat
    15121 accttcgata cgtatatcat cgttgggtta ttttacttag ttatcacgct accgttatct
    15181 tacttaatgg tttatcttga gaaaaaatgg gcggttcgtt catagaagga ggggaaatac
    15241 atggatttta gcggcgcttt ttcatggatg aatatccgtt ttttattgga aggattaaag
    15301 gttactgttg aagtttcatt gttttcgatc atttttagtt tcttgatcgg tggtctgaca
    15361 ggtgtgattc gttttgcgaa tatccctttc ttttccaaaa tccttggtgt agtgattgat
    15421 attatacgga atcttccatt attattgatc attttcttca cttactttgc cttacctcaa
    15481 atcggcattc agatgaacat cttctggtcc gctgtcgcag cattaacgat ttttgagtca
    15541 gctatgttgt cggaaatttt ccgagctgga ctcaatgctg ttccaaaagg acagatggaa
    15601 gcaggattat caacgggact gacttatgta gaatcgatgc gaacgatcgt gttaccgcaa
    15661 gcatttaaat caatgatccc ggcaattgtg agccaattga tttcactgat caaagatact
    15721 tcattagcag taatcatttc tttacctgaa ctttctcatc aggcacgtat tatttatgga
    15781 cagaacacca attatgtgct tccgatgttc atcatcatga cacttatgta cttcgtggta
    15841 tgttacgcgc tttctctgct ttccggttac ttggaaaaga gacggtacag ttattaatag
    15901 atgattcttt tagcggttgt ggcaaactaa agtcacaacc gcttttcctc tttccattcg
    15961 aaaaaaagag atttcgattg ttttaagcaa gtaagagcac ttgaaaattc acgctaaaaa
    16021 gtacacaata gggaggatag aatttattga aaaaaaggat gatacgattg acagataatc
    16081 gccctatcgg atttattgat tcaggtgtcg gcggcttgac tgtagtaaaa gaagccctga
    16141 aacaattacc gaatgaaaat attttatttg taggagacac agcacgctgc ccatatggcc
    16201 ctagacccgc ggaacaggta atccagtata cttgggaaat gacggattat ctggtggagc
    16261 aaggaatcaa gatgctggtg atcgcctgca ataccgcaac tgcggtggct ttagaagaaa
    16321 tcaaagctgc tctttctatt ccagtcatcg gtgtgatcct tcccggtact agagcggcag
    16381 taaaaaaaac acaaaataaa caagttggca ttatcggtac gattggtacg gtaaaaagtc
    16441 aagcttatga aaaagcactg aaagagaaag taccagaatt gactgtgaca agtcttgctt
    16501 gtcccaaatt tgtttcagtt gtcgaaagta atgaatacca ttcatcggtg gcgaaaaaaa
    16561 ttgtggcaga aacattagct cctttaacca ctaaaaaaat cgatacattg attttgggat
    16621 gcacccatta tccattatta cgccccatca ttcaaaatgt aatgggagaa aatgttcaac
    16681 tgatcgattc tggagcagaa acagtaggtg aagtatctat gctgttagat tatttcaatt
    16741 tgagcaattc accgcaaaat ggtcggacat tatgccagtt ttatacaact ggctctgcca
    16801 aacttttcga ggaaatagct gaagactggc ttggaatcgg acacttaaat gtagaacata
    16861 tcgaattggg aggaaaataa ggatgcgtca tgacgggaga aaagcccaag agcttcgcaa
    16921 actggagctt caaacaaatg ttttcaaata tccagaagga tcggtagtga tccgttttgg
    16981 tgatacaaca gttatttgtt ctgcaacaat agaaaattct gttccccctt ttttgagaga
    17041 aacaggaaca ggatgggtga cagcagaata tagtatgctg cctcgagcaa cgagtacgag
    17101 aaatcgtaga gaaagcagca aagggaaact ttctggtaga acaatggaaa tccagcgtct
    17161 tattggtcgt tccttgcgtg cagtagtaga cttggaaaaa ttaggagaac gtagtatcat
    17221 cgtagactgc gatgtgatcc aagcggatgg cggtactaga acagcaagta tcactggggc
    17281 atttgtggca ttgaaattag ccattgaaaa acttttaaga gaaaaagaat tgtcagaaga
    17341 tccaataaaa gaacatcttg ctgcagttag cgtaggaatc ttaccagatg ggacttgcgt
    17401 cacagatttg gattaccaag aagattccgc agcacttgta gacatgaatc ttgtaatgac
    17461 agaatctgga aaattcgtgg agattcaagg aactggagaa gaagcaacgt ttgatggtga
    17521 acagttgaat gaaatgctct tttttggaaa aaacgcgatt gaagatttga tcaaagagca
    17581 aaaacatgct ttgctaacag agtttgctca aaatgatgag cggatagaag aaacaaaaac
    17641 aatcatcatt gcgacaagaa atccaggaaa agcagaagag tttcgaaata tgttcaaaga
    17701 agcgggatat catgtaaaaa cattacttga ctatcctgaa ttgccagatg tggaagaaac
    17761 tggcagcaca tttgaagaaa atgcccgatt aaaagcagaa acgattgcac aattgcttga
    17821 tcagccagtc ttagcagatg attcaggttt gaaagtggat gctttaggcg gtatgccagg
    17881 aatctattct gcacgttttg caggggaaca aaaaagtgat gcggggaata acgccaaatt
    17941 attatatgaa ttgacagatg tgccggatga aaaaagaaca gcacaattcc attgtacgtt
    18001 agtattcgcg gcacccaaaa aagacagcct tgtagtagaa gcggaatggc ccggtcgagt
    18061 agcccgtata ccatctggcg aaaatggctt tgggtatgat cctttattta tcccagaagg
    18121 taagaaacaa acagcagcag aattatcgag cgaagaaaaa aacaagatca gtcatcgggc
    18181 tcaagcaatg aaaaaattga gtgccgagtg gaaacaatgg ctggaaggag agagatagat
    18241 gcggtattta gtagtaagtg ataatcatgg agacagagat atcctcgttg acttagtaga
    18301 tcgttatcgt tctgaagtgg atatcttttt ccattgtggt gattcggaat tagaagcttc
    18361 tgacccatta tgggatacat tccaagtggt tcaaggtaat tgtgattatg gaccaggatt
    18421 cgaacagaaa aaagtgatcc aaactggtca ggatacagta ttcatgactc acggtcattt
    18481 atcgaatgtc cgatttggtt tgacacagtt agcaatcgaa gctcgagctg caactgccga
    18541 tatggtgttt tttggtcata cccatcaaat aggctgtgag atggatcaga aaatcctgtt
    18601 tttgaatcct ggtagcattt ctcagcctag aggcccgatc caaatcccat catatgctct
    18661 tatcgaaagt ttagatgacc aaatttctgt tcaatactat aaccgtgccc atcaggcagt
    18721 tgaagagttg gccttttctt tttcgagaga gcgctgaata aaaaaactct aatttttaat
    18781 aaaaataagc tttattttat gaattttgca tccggtttcg gtaaaatagt agtgttgaat
    18841 gaaatagaaa tggaggccat cagatgattg gacctatagt aagagaaatg ctgctggaaa
    18901 atcaggaaac gtttttgatt cctgcagaaa atgtggcaaa tgttatgtat catcatccat
    18961 tgtcccatgg tttacttgtg ttatcgaagg tcggttattc caaaattcct gtattaggaa
    19021 aagatgatca ctttgtagga ctagtaagtt tatcagatgt ggtcaataaa atgattgact
    19081 tgcaaacaat cagcatggac ccgttagaag gactaacggt tgctgatgtg acagaaacag
    19141 atgttccgac aattgatgaa aattgggaac tagaagaagt actccatttg ttagtcgatt
    19201 cgcctttttt accggttgtc acagaagatc ggattttcaa aggaatcatt acaagaaaag
    19261 agctgcttaa agccgtaaac ttcatggtgc atgaattaga acgtcgtaat atattgtctc
    19321 cgaaaatcga agaattaaaa gaacaaatcg atatcgtgag ctaaagaacg agaaactaaa
    19381 agataatcaa aaaataagta gaaaaaacag cgtaccagtt ttgactttat cttgaaaaag
    19441 aggctgggac aaaagcgttt aactccaaga aataagaagg aattcacgaa aattgctttt
    19501 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gccgtttata
    19561 cgtgtttaga gcgtgggaca aaagtgttct ttacttttgt cccacgctct ttttagtgag
    19621 attcgttgca tcgtttgtcc aaattatttt ccgacagcaa ttggactgtt cggaatcgtg
    19681 aaaccagaag ctgtcaattc tttgacatag gagctcaaga actcttcttt taaggcatat
    19741 tgttttccgt tcaatgcgta gcagaccgta cgaatcgcaa aattgctgtt tccaagatcc
    19801 actaatccaa agatagaagg ttcagtttgg agctcttctt ggtatttttc agctaatgta
    19861 cgatttactc gatcaatgat ctcatagatt ttgtcatatc cttcttcagg aacaatccga
    19921 atatctaaaa gtacttgcat atttgcacga gagagattgc tgatcgtggt gatgtttcga
    19981 ttcgggatga aatggacagt accatccgtt gctttcagct gaagggtacg gataccaaca
    20041 gatacgacgg ttccttcaat cgttaagttt gctagcttga tgtagtcccc aacatccatc
    20101 tgttgttcca tgataataaa aaatccggta ataacatcac tcatgaaccc ttgggcacca
    20161 agaccgattg ccacacctgc aattccggca ccagccaaaa gtgatccgat cggcacaccg
    20221 atagcagata aaattgcata gataaaaaag aaaccaaggg tataatgaaa aatagtattt
    20281 aaaagactat gtaaagtctt taagcggctc tcgtttagaa tggctttttt agaatactga
    20341 ttaaaagact tatcaatcag atattttccc acacggtgaa gaatccaaaa aaggacgatc
    20401 aaaaaaatca gatataaact tttttggata aaggcagcca aaatggcgtc ccaatcaatc
    20461 ccgtcccaaa accgctgaaa cgcatttact ttgctgacag cttgatctgt caatgtctcg
    20521 ccagaagagt ctgtcgtacc ggcagttgct aataatgaaa acataaaatc ctcttttcta
    20581 aaatttgatt ctttctgtat tgtaccaaga agttttcaaa aaaggaaata gcaaaaataa
    20641 ctataatgaa ggaaaaaagc tgaaaataaa atgacaaata atatggataa tgataaaatg
    20701 tggagaaaat aacgaaggtg gtaagtatat gcaaatcgat tggaacaatc taggtttttc
    20761 ttatatcaaa actccttggc gttttattgc caaatggaaa gacggacaat gggaagaagg
    20821 agaattgaca gaagataatt acctgacgat tcatgaaggc tctccagcgc ttcattatgg
    20881 tcagcaatgt tttgaaggtc taaaagctta tcgtcgaaaa gacggcaagg tcaatctctt
    20941 tcgacctgaa caaaatagtc gacggatgaa tcaaagtgct agacgattgc tgatgccaat
    21001 ggtccctgaa aagatgttcg ttgatgctgt caagcaagtg gtaaaagcaa atgaagattt
    21061 tgtgccgccc tatggaacag gcgctacatt atacttgcgc cctttactga ttggggtcgg
    21121 tgaaaacatt ggtgtccatc cagctccaga atatttattt gtcgtcttct gtacgccagt
    21181 aggagcgtat ttcaaaggtg gactaaaacc aacgaatttt attgtatctg attacgatcg
    21241 tgctgctcct aaggggactg gtgcagcaaa agtcggcgga aattacgcag cgagtttact
    21301 tccaggaaca gaagcaaaag aactggatta ctcagattgt gtttatcttg atccagccac
    21361 acatacgaaa atcgaagaag taggtgcagc aaatttcttt ggaattacaa aagacggaaa
    21421 attcatcact ccgcaatcgc cctctatctt accaagtatc acgaaatatt ccttgttgca
    21481 gttagctgaa gaacgactag gactgcaagc aattgaagga gatattttca ttgatgatct
    21541 agatcaattt gcagaagctg gtgcatgtgg tacagcggct gttatttctc caatcggtgg
    21601 gatttatcac aataacaatc ttcatgtatt ttatagtgaa acggaagtag gaccagtaac
    21661 aaaacggttg tatgatgaat tgacaggtat tcaatttgga gacatagaag caccagaagg
    21721 ctggattgaa gtcgtggaat aatttcaaag taatgagttg aaaaaaacgt aaaaagaaaa
    21781 aatcaggaag agactttaaa aagttttttc ctgattttta tatgttttac tttaaagaat
    21841 gcaacaaaag tttatgaaag atggacacct ttgtcaacgt agataatatc accgatgatt
    21901 ccgctggaaa gtgggctgat taagaaagca caagcattac cgacttcttc gatcgtcaca
    21961 ccttgctgat caggcgtacg agattcagat aactggatca gtttttgata gtctttaaca
    22021 cctgtgacag ctaatgtttt gattgcccca gctgagattc cattgacccg aattccttta
    22081 ggcgagaact cagctgccaa ataacggata gcagcttcaa gagaagcttt cgcaataccc
    22141 atcatgttat aattcgggat tgcgcgttct gaacctagat aagtcatgct aacgatcccg
    22201 ctgtttggtg caagaaacgg ttgtgcgtat tttgatacag caattaaaga gtagctgctg
    22261 atatcttgag ctaattgata gccgtctctg gaaatatcag acacatttcc ggacaattct
    22321 tctttgttgg cgtaagcaac tgcatggaca aggccgtgga tctccccagc atattcaccg
    22381 atggcattca ttgctttttc gatgctttcg tcgctagaaa catcacattc tacagtgaat
    22441 gcttcttcgc cgaccaattt gactaatgat tttttcattc gttcattttg gtaagtatag
    22501 ataatttctg ccccttgttt caacattgct tcagcacaac cccaagcaat gctttttttg
    22561 ttagcgacgc ccataatgac gatttttttt ccttctagaa atgacataat gactctcctt
    22621 tgtttctgat aaaaatttga tagtcgtaaa aaaatagaga ataatagcta aaataacttc
    22681 ttgatttctt aattattagt caaaatgttt tgattgtcaa actatttttt cttaaattaa
    22741 aaaaagattg ctaaatattt aggaatatga tactttgata gtcaaagtaa tgaaaatggc
    22801 aattgcctga aagaaggagt ttaattatgt cttacgtatt atctgctact gaagtaatgg
    22861 aattgattcc taatcgttat cctatttgtt atattgatta tgtagatact ttagaaccag
    22921 gaaaaaagat cgttgcgaca aaaaatgtaa caatcaatga agattttttc caaggtcact
    22981 ttcctgacaa tccagtgatg ccgggtgcgt tgattctaga aacactggca caagcaggtt
    23041 cgatacttat cctaaaatca gaagagtttc taggaaaaag agcttatatc ggtgggatca
    23101 ataaggccaa attccgtcaa aaagttgttc caggagatgt gctgaaatgt cattttgaaa
    23161 tcaccaaaat gaagggatca gtcggtacag cgttatctgc agcatatgta gatgataaaa
    23221 aagtctgtga gtgcgagttc acgttcatcg tcaaccaaaa tgcttgacta taaaacctga
    23281 tatagcaaaa caaaaggaag cttacttgct ttttatgtaa aaagaagacg cctgaatcct
    23341 agcatacgtt gtcatctatg caggattcag acgttatttt tttatttttg ttttgcttct
    23401 tgcagtatat tgattttatc gatttttact tcttcttttg gtttgtctga actatctgtt
    23461 tcgatatttg caatagcatc cacgacatcc attccttcaa tcacttggcc gaagacagta
    23521 taatggccat ctagtgaagg ataaccgccg tttttataag cgtcgatgat tgcttgagga
    23581 tagtcgtctt ttaaaagacc atcatgcata tcatctgtat tttgaacgat aaagaactgg
    23641 ctgccgttac tgttaggatc ttgtgagcga gccatagata acgcacctct tatattataa
    23701 aggtggttgt tgatctctgt ttcaaatcct tctccccaaa tgctttcccc tccagtgcca
    23761 tcgcctttag gatcgccacc ttggatcatg aaatctttga tgacgcgatg gaaagtcagt
    23821 ccgttgtagt aaccgtcttt tgcatgtgtc ataaagtttt caaccgcttt tggtgcgtgt
    23881 tcgggaaata atttgatttt gattgtgcct ttattcgtca ccatttcgac cagatcttca
    23941 ttttcggcga catctttcgt caattgtggt aattccaatg cgttcaaatc gacgctactg
    24001 gcagtcgtgc tttccgcagt agatgtactt tttgaacttt ctgtagacga actagaagaa
    24061 gtcgtgttgt ttgcagaaca accagcagca aacagcaatg ctgctgctaa actaatactt
    24121 aaaataactt tatttttctt cataactgtt cctttccaat ttactatttt gttatcttta
    24181 atgataacaa aatagaggga aaatacctac ttaaattatt ggaatttatc atttatttta
    24241 aaagaaaaag aaaaggtgta gaccatttac tttgtgtttg ctataatgta agtggataca
    24301 aaaacgaaaa gatgcaggag gaaatggtaa tatgggaaaa ttaggaatct cgatttatcc
    24361 agaacggtca acatttgaaa aggacaaagc gtacttggat ttagctcata agtatgggta
    24421 taaacgggtt tttaccagtt tgctgcagat caatgatgat aaagaaaaag ttctttcaga
    24481 atttaaagaa gtagttgatt acgcgaatag cctaggcatg gaagtaatgg ttgatatcaa
    24541 tccagcgtta tttgaacagt tggagatttc ttacgatgac ttatcttttt tccataagat
    24601 gggtgcctac ggggtccgct tagatatcgg attcacagga gcagaagaag caaaaatgac
    24661 gcgcaatcct tttggtatca aaatcgagat caatatgagt tcgggcacga attacgttga
    24721 caacatcatg agttattcgc caaacacgga taatttatta ggttctcata atttttatcc
    24781 tcatcgatac tctggtttag gatacgaaca ctttgttttt tgttcagaaa aattcagaaa
    24841 atataatttg aatacgatgg cattcgtcaa ttcgcaatct gctgagtttg gcccttggcc
    24901 tacgcaagat ggcttgtgca cgctggaaga ccaccgagat cttgaaattg cgacacaagt
    24961 gaagcattta atccttacag gattgattga tgatatttct attggaaatg cgtacgcaag
    25021 tgaagaagaa ttgaaagaaa tggcagaagc atttaacgcg gattatccaa cattgaaagt
    25081 agatacggaa gaaggtatta cagaaaacga acgaatctgc ttgtttgata accttcatag
    25141 ttatcgaggg gatcgctcag aatatatcct tcgttcaacg atgactcgtg tatactacaa
    25201 agataaagat ttcccgcctc ataatacgcg agatatgcat catggggatg tactgattga
    25261 caatgaaggg tacggtcaat acaaaggaga aacacaaatc gccttgaagg atatgaaaaa
    25321 cgacggacgt gtcaatgttg taggaagaat ctccgatgac gaattattcc tccttgactt
    25381 tttgaaacca tggtcaagct ttaaattaat tgaaaataaa aaatagataa ttagttattg
    25441 cagaaataat ccgctagaaa tagccagtta tttctgcttt tttattttgt ttttttatag
    25501 gttatgattt gtcaaattaa gctagaataa aatgcttctt gtacatagct caaaaatatt
    25561 tcgattggtg ttctgtaatt caatgatttt cttggaatat gattacgttg attgctgaca
    25621 ctggaaataa atgtctgatt cacttttcta aaatccattg atttcggcag tccattacga
    25681 cgcagaatcc cgttagaatt ctcgtttaat gggcgttgag aaggtgttcc agggtccgca
    25741 aaatatatat caatatcatg ttggttacta atggctttcc agttagaaaa ttcttttcca
    25801 cagtcaaacg taatagattt aaagaagttt ttagggaagc gagaaaacca ttgattaagg
    25861 gcagtttcaa tatctaatgc cttacggccg ttgggtttaa tcgtgataat gactttagat
    25921 aatctttcaa ctaaagtaat gacggcactt ttatgatgaa tgccaacgat cgtatcacct
    25981 tcaaggtgac caaactcaga attgaaatca ggataattat caggtcgatc atggattgag
    26041 cgctgatact gttgttttcc ccgtttttcc tgatggccat tgggttttct tttacctttc
    26101 atcggtagtg tgtcaatatc aaatattcct ttagaaaata aacgataaag tgttctcata
    26161 ccg
//
LOCUS       DACDRK010000034        25605 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDRK010000034 DACDRK010000000
VERSION     DACDRK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.034"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            217..2277
                     /locus_tag="IWJ77_001511"
     CDS             217..2277
                     /locus_tag="IWJ77_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453119.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2360..2929
                     /locus_tag="IWJ77_001512"
     CDS             2360..2929
                     /locus_tag="IWJ77_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453120.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3104..3910)
                     /locus_tag="IWJ77_001513"
     CDS             complement(3104..3910)
                     /locus_tag="IWJ77_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453121.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4048..5562
                     /locus_tag="IWJ77_001514"
     CDS             4048..5562
                     /locus_tag="IWJ77_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9453122.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5965..6948)
                     /locus_tag="IWJ77_001515"
     CDS             complement(5965..6948)
                     /locus_tag="IWJ77_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9453123.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6953..7600)
                     /locus_tag="IWJ77_001516"
     CDS             complement(6953..7600)
                     /locus_tag="IWJ77_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9453124.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7685..8317)
                     /locus_tag="IWJ77_001517"
     CDS             complement(7685..8317)
                     /locus_tag="IWJ77_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9453125.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8333..8884)
                     /gene="coaC"
                     /locus_tag="IWJ77_001518"
     CDS             complement(8333..8884)
                     /gene="coaC"
                     /locus_tag="IWJ77_001518"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9453126.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8881..9618)
                     /locus_tag="IWJ77_001519"
     CDS             complement(8881..9618)
                     /locus_tag="IWJ77_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9453127.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9615..10604)
                     /locus_tag="IWJ77_001520"
     CDS             complement(9615..10604)
                     /locus_tag="IWJ77_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9453128.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10862..11404
                     /locus_tag="IWJ77_001521"
     CDS             10862..11404
                     /locus_tag="IWJ77_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9453129.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11421..12506
                     /locus_tag="IWJ77_001522"
     CDS             11421..12506
                     /locus_tag="IWJ77_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453130.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12518..13324
                     /locus_tag="IWJ77_001523"
     CDS             12518..13324
                     /locus_tag="IWJ77_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9453131.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13325..14152
                     /locus_tag="IWJ77_001524"
     CDS             13325..14152
                     /locus_tag="IWJ77_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9453132.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14149..15222
                     /locus_tag="IWJ77_001525"
     CDS             14149..15222
                     /locus_tag="IWJ77_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9453133.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15650..18346
                     /locus_tag="IWJ77_001526"
     CDS             15650..18346
                     /locus_tag="IWJ77_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9453134.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18423..18911
                     /locus_tag="IWJ77_001527"
     CDS             18423..18911
                     /locus_tag="IWJ77_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9453135.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18923..19417
                     /locus_tag="IWJ77_001528"
     CDS             18923..19417
                     /locus_tag="IWJ77_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9453136.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19438..20247
                     /locus_tag="IWJ77_001529"
     CDS             19438..20247
                     /locus_tag="IWJ77_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9453137.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20244..21065
                     /locus_tag="IWJ77_001530"
     CDS             20244..21065
                     /locus_tag="IWJ77_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9453138.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21116..23215
                     /locus_tag="IWJ77_001531"
     CDS             21116..23215
                     /locus_tag="IWJ77_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9453139.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23212..24330
                     /locus_tag="IWJ77_001532"
     CDS             23212..24330
                     /locus_tag="IWJ77_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9453140.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24369..25247
                     /locus_tag="IWJ77_001533"
     CDS             24369..25247
                     /locus_tag="IWJ77_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9453141.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25438..25590
                     /locus_tag="IWJ77_001534"
     CDS             25438..25590
                     /locus_tag="IWJ77_001534"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453142.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8098 a         4456 c         5415 g         7636 t
ORIGIN      
        1 aaaaggtaac tagatataac tagatataac tagatatatt acttaacaaa attaaaaagt
       61 acataagtta ttattccgat tttcttgaaa caataaaaaa tcaaaaaaaa tactctccta
      121 atagtccatc ttcttttgat tttatttata taatgaaatc atcttatgaa aattactact
      181 tcatgtttag acaagagtag ggaggactaa gaataaatgt gttcaaaaag acgttcaaaa
      241 caactaaata aacacgcttg tcgtttagat aaggaacggt cgtgttctga aaaacactac
      301 gttaagttta tgatgcgcac gatgctgatc agtttatctg taggtgtcta tatgcatgta
      361 cccgttcagg caacgacaca aaccatcaag gtcgtacgta cctctgtttc tgagaccact
      421 gattccccta tcactattac tcatactttc actgatgcaa atggaaaaca gctacttcct
      481 gcattgactt cctataataa agtcagttat cctccaacga taaaccacta ctggttaatt
      541 cctaacaaaa cattatggac atcggaaacc ggaagcagtg tgccgctttc tatcttttcc
      601 gatatgactg atcctacaaa aggtgatcag ttaaaacaga tcactgatat tttgaatcat
      661 gctttgacag atataaagga cggtggaagc gcacaagttt cttatgtata tgaaaaagat
      721 tattcacagt ttgaaagaaa gaaaatcgat gttctggcag ggacaaaagt ttcgatatct
      781 gatctggtag aaaggctgaa gaacgcagat ggaacacaag gagatatgtc caaagtaatt
      841 tcaaacaatg ggaatatcgt ggatacgact aatacaggaa catctactat ttccttgtcc
      901 tattttgatc caattgcttt aaaaacaatg actgcatctg ctgtgatcca tgttcgtgct
      961 gatcaaacag ctatcaaagg aaaagatctt cacttaagaa caggagagag ttacgcccct
     1021 aaagatcttg ttgaatatgt aacaaaaagc gatggaactt caggaaataa agaagaaatc
     1081 aaaatgaacg ggacagtaga tactacaact ccgggagaat atcccgttgt attaacttat
     1141 atagactcga aaacatcaaa tgtggcagaa acaacagctg tagtttatgt agaagcatct
     1201 gctgcttcat cgactgtcat cactcatttt atagatagtc aaacaaataa agaacttcaa
     1261 gcagaaacac atatgagtag tgtaaatcgt attcctgatt tgcgcctacc tgctgaattt
     1321 tctgatcgag tgccaaatat agatttatcg acttatgaac aaaatgggaa agtcacttct
     1381 atttctgatt ggatgcaagc aaacggatta gacagaaaaa gtcactggtc ggagatcctt
     1441 gaagtacttc actcagaact gatgaataat aggaagcaat caggagggga aattaccttt
     1501 acttatgttt atataccaga caatcgttca ttagaaggtc atgatcttat tgttcatcca
     1561 aacgaaaaaa taaacaaaga agagttgatc caatcagcaa caaattcctt aggtaaaccc
     1621 gtaccgttaa aggatgtatc ggttacaatc aatgaagtac cattggagga tggttatttg
     1681 accaaacaaa caggtgattt tacgattatg tatagttatc atgacccgta ttcactccaa
     1741 gaactccatg ctcagtcact attacatgtt ttaccattaa acaaacagat agatcaaaaa
     1801 gaacaaaaaa gacgagaaga aagcttgtca gaaaaaaaac aacaaatggc tcatgcagtt
     1861 tctccaacag ggaaagtaaa acaaacaaga agcctagagt tagcaagaat atctagaaaa
     1921 atctcagaat ctaataaaac gataaggcaa gaagctcatt tggccccaca agtttctgga
     1981 ttggaaaatg aaattttaga gcctaaaata aaacaacaat cagaaaaaga atttgttgct
     2041 aaacagtcgg aagatcagaa ggaatcagat gaccagaagg aaacgaagaa aaaacgcttg
     2101 tcaactattg attccaccga tgagcatctg cagccgcaag taccacttcc tagtccggga
     2161 acacccgcac ctggtggagg tattccttct tctgctttag gagatttcat gcgaggtatg
     2221 gcaggaacat gggtatacgc taatcgaaat gaatttgacg cagatgactg gttctgattt
     2281 atatctctat ctgttatagt caaataagta gctgagtcat atgttatttg gcataatgga
     2341 gacgaggagg gatagaaaaa tgttagataa aacgttacct tacgcagaaa tctggatgac
     2401 aagaccactt caacaaaatg ttccaatcac gcctttagcg gaaggttttg aatttcgatt
     2461 ttatcaaaaa gaagatgaat ctgcttgggc ggagattgag acttccgttg gggaatttca
     2521 aacgacagat gaagcacttg agtattttca acaaaacttt gctccttatc ctgaagaact
     2581 tgaaagaagg atgttttttg tagagacaaa aaaaggagaa aaagtagcaa cctgtaccgc
     2641 ttggcgaaaa aaatcaagag agcaagtgta tccggttttt cattggttag cagtgaaacc
     2701 agattaccaa ggaaaagggc tcgcaaaagc attgactgcc agagtcttgc aagaatttcc
     2761 taccctccat acggacggac caatctatct tcacacacag acatggagcc atcaggcgat
     2821 tgctctttat aaaaaaatgg gttttacttt tattgctgaa aatctagacg gcacacctaa
     2881 tccagattat gaaaaagtta tgcaggtttt aagtaaatta gataaataac gaaatggtga
     2941 aataagtcct cagtattggg ataaaaaacg aaatagtcat tcctcatatc ttactttttt
     3001 gagttaatac tatcggataa ctaatggcat gccatacatt caaaaaagaa ggggcagtga
     3061 caaaagtatt gtcactgccc cttctcttgt tttttagcat gttttatcgt ttgatttttt
     3121 taggaataaa attcttgatg aacgtaaatt tttggtaatc gtttcgacta tagaggtaac
     3181 caaatacact gaaaacaact gctccaataa aattaacaaa taaatccttc atagtatcca
     3241 tgattcccac gtctaagtaa ccattttgga tgattgtttt atctgtttta ccatctgcat
     3301 tttgactttc aatgattgtc cgagtgatat ctggaacatg atagacagtg tttccgtctt
     3361 tacttaactc cacactattg atttgctgga tgatcgtatc tttttgcata tcgaattgca
     3421 gtaatttatc tgctgaaaat tccacaaact cccacataac accaactgtc atcgaaaaac
     3481 agaaggaaac aactgctagt aataaaggag tcaagttcat ggtttttgta tttttattga
     3541 gcaaataaac aagggaaaag ccgacacccc cagctaaaaa tccgctgaat gcatgtagaa
     3601 aagtatccca cattgaaaaa tagccataaa aatcacttaa ctctcctaaa actgttgaac
     3661 taaaaataaa agataacaaa atgatttcta gcatatttgg aatctgtatc tttaatatct
     3721 tttccagaaa ataagggatt tcaaataaaa ttaatgtcag taataaaagc attgtatttc
     3781 cccagtgttg gttccagacc tgatccacta gcataataac gacgatgata cgtaataaaa
     3841 tatagattcc gtttgtgatt ctacctgcag tttttgtact gttcgtaaaa aaggttttca
     3901 aatgattcat aaaataactc cttcatgtat atccttaatt atactaagaa gcgtgtgttt
     3961 accaaagcta tatgacttaa agaaaaacag agttgatatt gtgaaaaaaa tcacttaata
     4021 tagtataagg agaaaagggg gatacaaatg aaaaaattag ttgctggttt attaacagcc
     4081 gttgcacttc ttgctatgag cgcatgcggc gcagatcaga aaaatacagc gcagacaagt
     4141 tctagtacga aggaatctgc cgaagtatca tcaggcgctt ctgaacaagt atatacagat
     4201 ccaaaagaaa tgaaagaatc ttatgatgtc gtgattattg gagctggtgg tgcagggatg
     4261 gcagcagcta tcgaagcaaa agatgctggg ttgaatccag taattttgga aaagatgcct
     4321 gttgccggag gaaacacatc taaatcatca agcggtatga atgcttcaga aacaaaattt
     4381 caaaaagaag aaggaatcaa agactcgaat gatgccttct atgaagaaac gctaaaaggt
     4441 ggtggaggaa cgaatgataa agaaatgctt cgatactttg tcgatcactc tgctgatgcg
     4501 gtcgattggc tagatcaaaa cggtatcaaa ctggacaact taacgatcac tggagggatg
     4561 agtgaaaaga ggacacatcg tccgtctgat ggctcggcta ttggtggata cttagtggag
     4621 gggttattga ataacgtcca tgagcgggaa atccctattt ttgtcaacgc agatgtgaca
     4681 gacattaaga aaaatgggga aacggtcaat gaagtgactg tacatgttca aggagaagaa
     4741 ccaaaagagg tcaccggaaa agcaatcgta gttaccacag gcggttttgg tgccagcaaa
     4801 gaatttattg aaaaatatcg tcccgattta gctgattata ttacgacaaa tcaagaagga
     4861 tcaactggag atggtatcac catgattgaa aaattaggtg gacaaaccgt tgatatggat
     4921 aaaatacaaa ttcatccaac tgtgcagcag gatgaggggg tcttgattgg tgaggttgtt
     4981 cgcggagaag gagcaatctt ggtcgatcaa gaggggaaaa gatttgtcaa tgaaatggat
     5041 acaagggata aagtatctgc agcaattaca gcattgcctg aaaaatcagc ttatttgatt
     5101 tttgaccaag gcgtgcgtga tcgtgccaca gctattgaat tttatgacca aaaaggatat
     5161 gtcacaactg gaaagacaat ggaagaatta gcagaaaaaa tcgatttacc aaaagaaaca
     5221 ctagcaaaaa cagttgatgg atggaatacg gatgttgcgg agaaaaaaga tgctcaattc
     5281 aaccgtgcca cagccatgga acatgactta tcgtctccga attattatgc gatcaaaatc
     5341 gcaccaggaa tccattacac gatgggcggt gtgaaaatca atacgaacac tgaagtatta
     5401 gataaagaaa accagccaat tagggggtta tatgctgctg gtgaagtcac aggtggctta
     5461 catggaaaca accggatcgg tggaaattca gttgcggaga tcattatttt tgggcgacaa
     5521 gcgggcatac aagcagctcg atttgcttcg gaaaatgaat gaaatagaac gtaaataaaa
     5581 agaggttgtg aaatcagcgt tttgagctaa taccgagggt cagctgattg aacactgttt
     5641 agtgaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aagtaaaaac
     5701 agcttcttat gtttttaact tggttggact tattaccgca gatgcaagct tttgaacacc
     5761 gtttattcgg aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     5821 cacgtataaa cggcggaagc agaagaaatt ccttcggaaa taagctgaaa ttcacaaaaa
     5881 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
     5941 gcctcttttt tgatcagcat gattttattc agttatcttc aaaatgattt ttccgatgtt
     6001 tttgtttgct tccatatatt catgcgcttg tttgactgcg ctgaattgga atgtacggtc
     6061 aatgattggc cgaatcttgc cattttctag aaaaggccca gctttttgca taaaagtttg
     6121 tgcgagctct gctttatact gatcacttct aggtgtcaat aatgtaccaa gcagttggac
     6181 acgtttccca atcaatacac caaggtctat ctcaggcacg atggtcccac ctagcatacc
     6241 aatcaagacc caacgtccat caacggctat agaagctaaa ttcttttccc agtaagaagc
     6301 gccgatgaaa tccaaaataa catcgactcc ccgaccttca gtgatttttc gtacctcttc
     6361 ttgaaagtct tgttttttat agttgatcaa ttcatctgca ccaagttctt tacaaaaggc
     6421 gagtttttct tctgatcctg cagtaacgat gattttcgcg tgagaaagct gtctagctaa
     6481 ttggatcgct gctgttccca caccacttgc tcctgcatgg atcaagacag tttcgccttc
     6541 ttgtaatttt ccaagccagt aaagtgtttg ataagcagtc aaaaaaactt ctgagattcc
     6601 agcagcagaa acatagtcta gcgtatcagg gagaaggatt gcacgatcgg caggcatcac
     6661 agcatattcg gcataaccac ctagattgac taatccgtat acttttgtcc caggagtaaa
     6721 agtggagata tcacttttat tctcaactac ctctccagct acttcgactc ccaaaatagg
     6781 gtagggaggt ttcaaagaga ctgattctct tttcataata tctgttcgat tgacagctgc
     6841 tgtatggact ttgatcagta attcgccttg ttttggttta ggaacaggca cttcaatttg
     6901 ttctaataca tctacagaac caggatgctt gattgaaatg gcacgcatgg atctattcct
     6961 cctttgccca ttcttggaat gctgtattca actctttgct accttcagaa agtactagag
     7021 tcgtacgaga aatatccgtt tcttccacta gagcggcaag tacagcagca actgtagagc
     7081 gagtgatcgt ttttgatgcc ttctcattag gcttagttaa ttgaatggaa gtaacttctt
     7141 catcgttagt caatgtgact ggttgaagaa tgacaaaatc taagttagtt cgtttcagcc
     7201 attcatctgc ataatgttta gtaatataat aatctgtcat ggactcaggc cattttgctg
     7261 gttcatctgc ataaacagca ctgaccatca gatagcgaga gacatgcgca ttttcagctg
     7321 cgatcactgt tttgattgcg ccatccaagt ctacttgcag aagattcttt ccttgagagc
     7381 cggcagcaaa aatcaataca tcgatgcctt tgaatgcttc ggtcattttt tccacggacc
     7441 aagttaaatc aaaagagaca tacgaaacat tctctttttt tactaatgtt tgggaagcaa
     7501 catctctcac ccctgcaaaa atttcatggg aacttggagc taaatctttt atcaggtgtc
     7561 ggccaatttg tccgtttgca cctacaacaa aaattttcat ttggatcatc ctttcgacgt
     7621 caaatctttc cattcaatag taacaccaaa agagaaaaaa atcagagatc ggctacgatg
     7681 actatcagtt agtaagagaa gggtgcttga agtatcttaa caagacattg catactgctg
     7741 cagtcgcaag acctgcgacg attccttcag gtataccact tgtaaaaata accgctagaa
     7801 tagctgaata aacagcattt aaatttgttc cgatgacttt ggcataatct tgctggaata
     7861 aaaagtagat catattcatc actaaaatcg tattggtcaa tgaaccggaa agtccagcta
     7921 agaataaaga aacggaatct gctttttgtt tcattagttt gcgcagacct ttataaacga
     7981 aataaggaac caccccaatc aagatgcgag gaataaacgc gatcagcaag gctttccagc
     8041 ttccatgttc tgttccgatg actgggataa acggagaaaa gacaaaagac aaaggtgttt
     8101 ggataactgt gctacggatc atgctgatca gtccaaatgt accgccaaga aaagcaccta
     8161 gtcttggtcc taaaacgatc gaagcaatga ttacaggaat atgcatggtt gttgcattga
     8221 ttggaccgat agggataaaa cctaaaggcg taacagctaa taaaatcaaa atagctaaaa
     8281 acatcgcagt taatgtaaag ttttttgtat ttttcataag tactcctttt ttctaagcat
     8341 ttctttcatt aagagtcagc atgacctgtt cgatgatcgt ctgaatatca gctaaggcac
     8401 cttttccaaa atctccacaa gctaaaagag attctcttgg ttcaatttcc tgatagccga
     8461 cagatctcaa tgtttccaga ttacgctgag tgatcgggtt ttgatacata ttcgtgttca
     8521 tggcaggggc aatcaatttt ggtgttccca ctggaagcgc cattgcgact gttgaaagaa
     8581 gatcgtctgc aatgccgttt gctagcttgc cgataatgtt tgctgtagca ggcgcgatga
     8641 gaaacaagtc agcttgtttt gctagttcga tatgattgat tacctgcgga tttgattctt
     8701 gcatgacgtc tgtatggact gcgcgcttcg acagagattg caaggttaat ggcgtgataa
     8761 aatgtgtgct gcttttagac ataatcacat ctacctgggc acctaatttg acgaattgac
     8821 ttgtaagatc agcgcttttg taagcagaaa tacttccagt aacgcctagt aagatctttt
     8881 tcattttgat tcctccagtc taattttctc agtaatcaat gcagcaatct cagatttact
     8941 ttgagcttct tccactgttc catctttaga taagagatag ccgtggtgtg tctcgccatg
     9001 gatttccgtc aaatcatttg cgaaaataaa atcagttttg ttatttatca gactattttt
     9061 tgcgactgcc agaagagatt cctttggtac atctactaat agtttgaaac caatgatttt
     9121 tgcttttggc tgccattcac gtagataaga aatgattttt ggtgttttct tcaaaacgag
     9181 cacaagatgc tcggtgttag aagagatctt tgaaacagtg gtatctgtct gttcattttc
     9241 cgcaaaccaa ttgtccagtt cttcttgcgt tgaagaagtg ggtaggtttt ttgccagctg
     9301 ttcttctgaa aaactaaaag caggagtaaa atcactgact gccatactgt ggataatcgc
     9361 atcgtagtgt tcttttttcg ttagttcttc taattgtaaa aataagtcca gtgcagattc
     9421 gatcgtataa atcgttatat tggagtgtct tttcggtttc agtgcttgtt tcgtagtcac
     9481 gtaatcgatt gtggtatccg gattggcagc taaataatca gctaatgctt gccctaatcg
     9541 tcctgtcgaa tgatttgtaa tagcacgtac ttgatcgatt ttttcagtcg ttcctccagc
     9601 tgtgattaga atcttcattt atggtcacct acatttacta gtgttcttcc ttggtgtgtt
     9661 ccagcaagta gtttagatgc tgtttcaggc aattgatcca gagtaatctc ttggacgacc
     9721 agttcatctg caatctgcaa atccgccatc tgttgccata aagaaagacg ttgatctatt
     9781 ggtacattga cagaatcaat tccaatcaac tggatgtttc gcagaataaa aggcaagaca
     9841 gtcgcgttta gtttgattcc cccagcattg ccacataaag ctactgcacc gttataagag
     9901 atcaatggaa gaagactgct tagctgctcg ccacctactg tgtcgatgac atagtcgatt
     9961 tgctgttttc ctaaagcttt tgttgtctct ggcagaaatt catcaggtcg tacgacttgc
    10021 aaagcaccta gactttttag ccatgtgaca gactcttctt ttctggaaaa ggcaatgatt
    10081 gactggaatc ctagtttatg taacagggct atcgctgtac tgcctacacc gccagaagca
    10141 ccagtaacga caactttttt atcggaggtc ataccttgac ggattaaagc gttcacacac
    10201 aacatagcag taaaccctgc tgtaccaagg atcatcgctt gacgcagcgt catatttttt
    10261 ggtagaggaa ctaaccagtc tcctggtact ttttgatatt ggctataacc gcctggatgg
    10321 gtgacgccaa gcccaaaacc agtagcaagt acaagatctt cttttgaaaa acgtggattg
    10381 ttactttgga cgacagttcc ggataaatca atccctggag tcatcggata tgttcgtatc
    10441 acctgaccag actcgctcat tgccaatgca tctttataat tgacatcaga ataggcaatt
    10501 tttaccaaga cttcattttc agccaactca gtaaatggca gatcactgat tgctgtatgg
    10561 aaaacaggtt cctttattac ctgaaaagat cgaaaagcgt tcattcgtga gacactcctt
    10621 ttaaaatttt cttttatcat accataaagc ctactctttt attcttgttc ttccgtaaat
    10681 ttagacaaaa attaggtgaa aaataaagaa tcaaacaact agaatttatt ccaaaagata
    10741 aagaaatatt cagcaaaaaa aattttacaa agggttgcct ttttcaataa atttcgtata
    10801 ctaatacgag tggtagtaaa caggaagttt actattagaa aacttcgatt cggaggaaat
    10861 tatggaaatt ggtgagaagt taagaaattt gcgcatccaa aagaatttga cacaagaaga
    10921 actaggcgaa cgcacagatt tgtcaaaagg gtatatttcc cagttggaac gagatttaag
    10981 ttcaccatca atggaaacat tcttttctat tcttgaagtc ttaggtgtga ctccagaaga
    11041 attttttcat caagaagcag cgaatctgca agtggtttat tcaaaagaag atcatacggt
    11101 ttatttcgat gaagaaaacg ggtatgaatt ggaatggctc gtggctgatt cgaatgaaaa
    11161 agaaatggag cctgtcttgc tgacttttga aggtgcagga gaatataaga cgtttgaacc
    11221 ttcattatct gaaacgttta tttttgtatt agaaggggaa ttagagttaa gcttaggaga
    11281 gacggtctat gtggcaaaag caggacagtc gatctattat caagcgacaa aacaacatca
    11341 attaaggaat catctcaaaa aacgaacaaa agtattgatt gttgcaacgc aatcatattt
    11401 gtaatatagg gggtaaaaac gtggggaaac caatcatttc atttgatcaa gtagtgaaac
    11461 gctatgatga tgaaacagta ttaaaaaaag taagttttga gatcgaacaa ggaaaatttt
    11521 atacactgct tggtccttcc ggctgtggta aaacaacgat cctacgtatt atagctggat
    11581 tcaccgaagc tacagaaggg gacatctatt ttgaagggaa acggttgaat gatttgccag
    11641 ctaataagcg acaagtaaat acagtcttcc aagattatgc actttttcct catatgaatg
    11701 tttttgataa cgttgctttt ggattgaaga tcaagaaaat gtccaaatca gatatcgaaa
    11761 agaaggtaaa agatgctttg cgtatggtcc agcttcctgg atacgaaaaa cgggagatta
    11821 gtgaaatgtc tggtggtcaa agacagcgtg tagcgattgc acgagcaatt gtaaatgaac
    11881 cgaaagtatt gctattagat gaaccgttat cggcgcttga tttgaaacta cgtacagata
    11941 tgcagtatga actgcgggat ctgcaacaac gattaggaat cacgtttatt tttgttactc
    12001 atgaccaaga agaagcgttg gcaatgagtg atgagatttt tgtcatgaat aaagggaaaa
    12061 tcgtccaaag tggtacaccc gtagatattt atgatgagcc aatcaatcat ttcgttgctg
    12121 attttgttgg ggagagcaat atcgtggacg gtgtaatgat cgaagataat ctagttgagt
    12181 ttgttggtaa gcaatttgaa tgtgtcgatg gcgggatgcg tctaaatgaa ccagttgaag
    12241 ttgttttacg cccagaggat ttgacgatca cgacaccaga taaaggaaaa ctagtcgtaa
    12301 cggtcgatac tcagctgttt cgtggtgtcc attatgaaat catctgttat gatgaacagc
    12361 aaaatgaatg gatggtccat tcaacgaaaa aagctaaaga aggaagcaaa gtaggattgg
    12421 cctttgagcc agaagatatc catgtcatgc gtttcaacga gtctgaagaa gaattcgatg
    12481 cacgtcttga cagctacgaa gaataggggg gacaagaatg aaaacaatgc gcaggatcta
    12541 ttccgtccct tatattttgt ggctcggttt attcgtgatc gcgcctgtat taatgatcat
    12601 ttatcaatct ttttttgata tgaatgggca attcacgctg aataattatc tatcttattt
    12661 tacgtcgggg acatcgcttt ctatgacgat caattctgta tggtatgcat ttttgatcac
    12721 gtttttcacg ctgatcatca gttacccgac tgcttatttt ttaacaaaac tgaagcacaa
    12781 gcaattatgg ctgatgctga ttattttgcc tacatgggtg aatctgctgc ttaaagctta
    12841 cgcatttatt gggattttca gtgtgcacgg cagcgtgaat catttctttg attttatcgg
    12901 gatcgggccg aaacagatat tattcacaga cttcagtttt ctgttcgtgg caacgtatat
    12961 cgagattcca tttatgatta tgccaatttt caatgcattg gaagagatga atccttcttt
    13021 gatcagtgct agtagagatt taggcgcgaa tagctttgag accttccgac gagtgatttt
    13081 tccgttatca ttaaatggtg tgaaaagtgg tgtccaagca gtatttattc cttctctttc
    13141 tttattcatg ctgactcgtc tgattggcgg aaatcgggtg atcactttag gaacggctat
    13201 cgaacagcat ttcttggtta cacaaaactg ggggatgggt tctacaatcg gtgtgatttt
    13261 aatcattgct atgttcatcg tgatgctcct gacaggcgag aagaaaaaag gaggtcgctc
    13321 ataaatgaaa aaatttaaat ggtcgtatct ttatttaggg atcgtctttt tattcttata
    13381 tgcaccgatc ttctatctga ttttttattc cttcaatgaa ggaggaacga tgaacagctt
    13441 tacaggattt acttgggaaa attatgaagc tgtttttgaa gatacacgtt tgatcattat
    13501 cgtattgaat acgttcatgc tggctttctt atctgcattg atcgccacga tcatcgggac
    13561 atttggggct atgggaatct attacacgaa aaaacgacgt gcacggacag cgttgttgag
    13621 catgaataac atcttattag tctctcctga tgttattatc ggtgctagtt tcttgatttt
    13681 ctttacacta ctaggattcg gattaggttt tcagagtgta ttgctttccc atattgcttt
    13741 cagcattcca atcgtcgtct tgatggtctt gccaaaattg caggaaatga acgattcaat
    13801 ggtcgatgcg gctagagacc ttggtgctaa taatttccaa gtagtcaaaa atatcatttt
    13861 gccttatctt tctccaggaa tcatagctgg atattttatg gcatttacct attctcttga
    13921 tgattttgcg gttacatttt tcgtcacagg aaatggcttt acgacgctct cagtagagat
    13981 ctattctcgt gcaagacaag gaatcagttt agaaatcaat gcgctaagtg cgttagtttt
    14041 cttgttctcc atgatattgg tcattggata ctactttatt agtaaagaaa accgtccaaa
    14101 acggctgaag aaaaacagaa gagtacgcga gggggtggca cggctgcgat gaaaaaatta
    14161 caatctctgt ttttaggtat tctcatcatt attgcggtgc tgctgcttgg tgttcgtcaa
    14221 cttgaaaaaa caagtggcat gagtggagca aaggtactta ccatctataa ctggggagat
    14281 tatatcgatc ctagtttgat cacgaaattt gaaaaagaat atggatacaa agtgaattat
    14341 gaaacatttg attctaatga agcgatgttc acaaaaatcc agcaaggtgg gacaaactac
    14401 gacatcgcta taccaagtga atatatgatc caaaaaatga tcaaagaaaa actagtactt
    14461 ccattagatc attcgaagat caaaggatta aataatattg atccacgatt tttagacttg
    14521 gattttgatc cagataatca gtattcgatt ccatattttt ggggaacttt agggatcgta
    14581 tataacgata aattctttga tgcaaatcaa attaagcatt gggatgattt gtggaagccg
    14641 gaattaaaag acagtttgat gctgattgac ggtgcacgag aagttatggg gctttctttg
    14701 aatagtttag gctattcttt gaatagtaag aatatggcac agttgacaga agcagcaaat
    14761 aaattgaatc atttaacacc aaatgtcaaa gcaatcgtgg cagatgagat caaaatgtat
    14821 atgatcaatg aagaagcctc cgtggcagtg acattttctg gcgaagctgc cgatatgatg
    14881 tgggaaaatg aacatttaca ttatgtgatc ccaccagaag gatccaatct ttggtttgat
    14941 aatatcgtta tgccaaaaac cgtgaaaaat aaagaaggtg cctatgattt catcaacttt
    15001 atgctagaac cggaaaatgc ggcacaaaat gcagagtata ttggttattc tacaccaaat
    15061 gaagaagcaa aagcgttatt gccaaaagac atttcgtcgg acgaacaatt ctatccatct
    15121 gatgacacga tctcccatct tgaagtctac aaagatctag gatcgaaata cctaggcatc
    15181 tataatgacc tattcttaga attcaagatg tataggagat aatggcaaaa ctcagtaaga
    15241 atggggactt atcaacgttc taaagagcta aaaatcgtcc tttttctatc tgagttttcc
    15301 aatcttctac caaaagcgga tgaaacgccg ctttttttgt gttattttat tttttctacc
    15361 acgtttttct accagagaaa agtggacaaa accattgtaa aatagggccc gagacgggct
    15421 agtaaatcta agattgaaaa agttacagaa ggataaagta attggaaact atgcaaatat
    15481 acttaaccct tctgtaatct attaataaaa ctaatatatg aatccagttc aatatttgta
    15541 gctatgaaaa aacataaaaa tcatattaga taaaaactaa attggtagat aaattatttt
    15601 gtcagatatc tgaggcaaat attcgactat ttgtgtttca aagtaaaaat tggaatacaa
    15661 atatagaata gacatattgc agccgatagt ttattatgct tttagacaca ttaaactgga
    15721 ggaaaaaatg aaaaagagcg ctaaagatcg agtttttgat gagctgatac gactaacagg
    15781 gaatatgtct tctgtcactg ccgagaaaat ttcgaagaat ctaggtatct ctcgtcagaa
    15841 tgccagccat tatttgacga gattagtgga agataaaaag gtcgagaaat taccgggaaa
    15901 gcctgttttg tggaagcctt tagatgaata tgcggttgta gataatacgg agcaaataaa
    15961 cgaagccttt cattctgtag ttggtcatga cggaagtctg agagaggtca ttcaaaaatg
    16021 tatagccgct gtcaaatatc cgcctaacgg actcagtgta ttaataaatg gagccacggg
    16081 tgttggaaaa agttttttag ccacaaaaat ttttgaatac gctatccatg agcaaattat
    16141 tgaaaaagat gcaccatttg ctatcttaaa ctgtgccgat tatgcggaca atcccgaact
    16201 gctttcggca acactgtttg gatacaagaa gggggccttt acgggggctg aaaaggatac
    16261 cgatggttta ttggcaacgg ccaacaacgg ctatcttttt ctcgatgagg tccatcgcct
    16321 atcgaaagaa aatcaagaaa agctctttct atttattgat actggaaact atcgtccagt
    16381 cggtgaaaat gtaaattggc attctgctaa ggttcgtttt attttcgcca cgacggaaaa
    16441 aggtgaaaat tatttattag acacctttga tcggcgtatt caaattagtg ttatgttgcc
    16501 aacttttgaa gatcgcccca ttcgagaacg attagagctg attcaattat tttttcaaaa
    16561 tgaagcggat gttttacaaa aagatattat tgtttcaaga gaagccttgt ctttaatgtt
    16621 atctcatccc tttagtggga atattggtaa gctgaagaat atcattaaaa tcagttgtgc
    16681 ggatgtgtat agtcgaacac aagaagaacc attaacgatt gggaaaaatg agatcttgat
    16741 tcagttaaat atgctggaat ccgaagtaga aagcttgccg attgctagtt tattgataaa
    16801 gaaaaatcaa aagataaaaa cggatcgaat caaaccgttt gattatgatt cgattttgga
    16861 aaaaattact cagctgataa atgaaggtaa tttcaatgaa aatttttctt cgataaagaa
    16921 tgaagtacag catttggtgc atcacattga taaggattct tttttatcaa tgagtagaaa
    16981 attgatgatt cagatgttta gaaaggtctg ggtatccgtt ttaggaaaaa cgtacggatt
    17041 gacgacgagt ggacctgttg cagaaattat tagcagatcc tatgtcattt ccccaagcta
    17101 taagacagac ttaaccggga taaatcgtac attagctgca aatctacctc ggacgactta
    17161 cttatgtaat cagttcttgg agcatttacc accgctctca gaaaaggacg agaactttct
    17221 aagactgctt attgtcgtga ctttatctga ttatgtagat gaatccatcg agctgaaggg
    17281 cctgttgctt gctcacggag atagtacggc tagcagcatt caagcagtag tgaatcaatt
    17341 atgcggcaat tatgtttttg aagcgttgga tatgccagta gagagtaatg tgtcagagat
    17401 tgtcgaatcg acaaggaatt tcgtgaaacg gcaagtgaat acagatagtc tagtgctgat
    17461 cgtagatatg ggctctttaa atcaaattta cacacagatc aagaacgatt taaaagggga
    17521 gttacttctt ctgaataatt tgacgacttc cattgcactt gatattggct tgaagatgac
    17581 gatgaatgcg ccatttaaac agattgcgga ggatgcagca gacaagtatt caatcaatgt
    17641 tcaatacttt gaaggctttt ctcaaagtag caacattatc atttcctgta tgtcgggttt
    17701 ggggatttcg gacaaattga aagatgtttt tcgtcatcat ttatcggatg aatccatcga
    17761 agttttcacg aaggactaca gagaactacg tgaattaatc gaccaaaatg atgaaactta
    17821 ttttgacaag acaaaattgg tcatcacgac ttcggatctt ccacgttcct tttcaattcc
    17881 taatattaat gtttatgaca tcttagacgg tgatggagca gccaatttgg agcatatttt
    17941 gagtcctgaa atgaagccgg cagaatttaa ccgcttgatt caggagctag taaaattctt
    18001 ttcgttggaa ggaattgcgg accgtttgaa ctttctgaac ccaacgatcg tgatgagtga
    18061 agtggaaaca gtcatttcta aatatgaaaa ttactatcat ttcactttaa atggccgtgt
    18121 aaaactaaat ctctatatgc atacggcatt gatgatggaa cgtttattct tggctcggac
    18181 agaaaaatct gaagaaccga attatgattt gaaaccagat gaagatgaat tttattatgt
    18241 ttctagaagt atttttcaat caatggagat gaaatataat tttaaagtca atgcttatga
    18301 gctttcgctg ctctatgaat tattaggacc gtatattcaa aaataaagtg tctaaaaaag
    18361 tgtctaatct gtttttttta ggttagacac tttttttaat gtgaattaac gtgattaaac
    18421 caatgttttt aaaagttggc acaggaattg cttatataaa agtgaacaga aagaaaggaa
    18481 ttaaccatat gaagaaaatt ctaatagcga ctcacggtca cttagcttca ggctttctta
    18541 gcagtattca attactgacg gggaaggttc aagagattac ggtgattaat gcttatgtgg
    18601 atgagtgtga ttttgaacag gagttgaatg tatttgtaaa ccaggtgaac gagcaggatc
    18661 agatttttgt ttttaccgat ttatttggcg gaagtgtcaa tcagaagatt accaaaacct
    18721 ttttggagaa agagattgct gtaactattt tgtctggctt taatctgcca attctgctcg
    18781 aaatagtatt ggcaaccaag gaattaacaa aggaggatat tcaggaactg gtaaataaat
    18841 gccaacagga attaaagatg accattcttg atgaggtaca tgtagtaaat gaagaggaag
    18901 atttttttta ggaggaagaa aaatgattaa agcagtgcga attgatgaac gattaattca
    18961 tggacaagta gcgatgactt ggacaaagag tctaaattta accgggttag tagtagcaag
    19021 cgatgaggcg gctaccaacg atattcaaaa gatgacattg aagatggctg caccatcgaa
    19081 tgtcaaagtc attattaaaa cagttgatga cgcgattcat ttgttaaaag atccgcgggc
    19141 gaaggacatg cgcttgatgg ttttagtccc taatgtcaaa gatgcggtaa aagttgcgaa
    19201 agctttccca acggaaatcg aattaatgaa cgtggggaat gctgggaaaa tgacaccgcc
    19261 atcggaagat aagaaaacct tatcaaagga agtgatgtta acgacgtcag aaattcaaga
    19321 cttaaaagaa ttagttgaac tttatccaga taccgttttt cagcctacac cggcgatgga
    19381 aaaacgttca gctatctcta ttttgagtga tttttagaaa aaggaaggag aaaaattatg
    19441 tatgatgcat ttgtagtcgc tttggccgtt tttattggaa ttgctggaca tgaaatgttc
    19501 gggatgtcca tgctgagtcg tccgattgtt gttgctccgt tagcaggagc gttgatgggg
    19561 gatttgcaga cggggttgat tgtcggggcg tcactagaag ccatctttat gggtgtcgtc
    19621 aatatcggaa tctctagtac ggcggagcct tctttggctg ctgggctggc tacagcgttc
    19681 gcaatcaaaa tgggaggaaa cgtggatgcg attattccca ttgtctttcc actagcagta
    19741 ttaggattac agttaatgaa tatgattttt tcttttgtat gtggaccgat ggctgctaaa
    19801 tttttcaaat ttgcaaaatc tggtgatcaa aaaggaatca tcaatctgca cttctttatg
    19861 tggtttgtcc actatgcgct ttatgctctt attccattct ttgccgtttt atttggagca
    19921 gatgttgtca aaagcgtctt ggataatatt ccagaggtca tcatgaacgg attaacagtc
    19981 gctggaaatc tgctgccggc agttgggatg gcgatgctgt tacggatgct ttggagtaaa
    20041 aatattgctg tgtggttttt ctttggggcc gttgttatgg cgtatctcga gttaccattg
    20101 attgcaattg ccgcagttgg aacgattatt gcaattgtta tagcccaaag agatctacaa
    20161 ctaaaaaata tatctgttca tgcagttcaa agcgatgctg ctgtaaatgc aacgcttgac
    20221 caagaagagg aggatttctt tcaatgaaat tagctgatgg aatttctaaa gatgagaaaa
    20281 aattggttcg gaagatgttt tggcgctcga tgactatgta tgcttcggtg aatccggtga
    20341 cgatgggcgg cggtggtttc gcctattcta tgatgccctt cattaatcat ttctacaaaa
    20401 gtgaagaaga tcgagctgcg gcgctggaac gtcatacaca gtatttttcg acgaccattc
    20461 cttgcgcgtc ctttgtcatg gggattgcgg catcgatgga gaaggaaaat tctgaaaaag
    20521 ctggttttga cgtggaaagt atcaacgcga tcaaaacgag tttgatggga ccattggctg
    20581 gaattggcga ttcattgttc tggggagttt ggcgtgtcgt taccgcggga ctagcgatca
    20641 acttagccaa tgccggaaat attttggcac caattatctt tttagttatg ttcaatatac
    20701 ctaattactt gatgcgatac tatggcggcg ttttaggcta ttcattaggc tctaaatata
    20761 ttgaaaagct gtatgcgaac gggttaattg aaattctgac aaaagctgct agtatcttgg
    20821 gtctgatcat ggtaggtgca atgacgatca gtatggtcaa attcaatacc gttttgagct
    20881 tttcaatgaa gggcgaagaa ctaatgaatg tccaagaaat cctcgatagt atttttaaag
    20941 gaattgtacc gttattggta acattgagct gtttcaaatt actagataaa aaagtcggcg
    21001 tcatcaaaat tatttttgga atggtcgtct tgggaatttt attgtcatta cttggaatcg
    21061 catagtagga agctgcagct actaggctgc agttttttta gcaaggaggg aaaagatgac
    21121 tattaatgaa gtaatttatt cacccaaaca cccatttttt aatgctgagt taaacgacgg
    21181 aaatgtgact acttcaaacg aatacagcta ctatcggaat attcctgatg taaagggatt
    21241 gaccttcgat gtaagtaatc aaaagacatt agcaaacctt gatgtttgcg ggacgattca
    21301 aacgttgacc ttttatcgag aaaatttact aactgaggaa aaaccaggtg tttgggtcaa
    21361 taaacagctc tctcaaacca attcgttatc cttaaaggtt gaagtaaacg ggattgtcta
    21421 ccatttgtca gaatcggatc atcgtattga agtcgatgtg attcaagact gtttgcctag
    21481 atatattcat cattattcga cgtttaaggt cattgtagta tcctttgctc ccatactggc
    21541 taataagcgt ttaagtatgc ttgttcaaca ggtttacatt gtgaatgaaa cacacgaaag
    21601 ccttcaagtt tcagtgaaag atgtgccttc gtatcaagag aagtattcgg atcagcaaaa
    21661 tgtgttgatt tctcaaaaag gaaataagga cacgatcgca caacaggaag tggcaagctt
    21721 cgccattgct tttgtggacc caaatgcttt tgaagaagta atgacttttc aggaatctga
    21781 tatcgaaaaa tggctgaagg aaacattgaa ctattttgct cagctatttg ggcagctgtc
    21841 aatgccaaac gatagagttg tccatctcta tcgtcgtgca ctttatcaaa gcttttctag
    21901 tttcggtatg aatcgacagg gacaggtcgt cggcagcaat tggggcagct atcctgctac
    21961 taatcgaatt tggaataagg atatgtatta cgcatcattg ccattcacca tgtttgaacc
    22021 ggagttatgt caaaaaacgg ttctatggtt tgatcaatat ggcgttaagt ttcctggttc
    22081 aaaatttaca ggaggtatca atcattctct gagtaattcg ttatccagtg cgttactggc
    22141 tagtctttat tttgaacata ctggggatac aaaattcttt gagcggtgtc cgcaggttct
    22201 actgaatgct tcaagaatta ttgaggacat attagctcaa cgtcggtcag gggaaccaat
    22261 gctttttcca tctgtttggt tatcagacgc ctttgcactt ggaaagtatc ataccggcag
    22321 caatctttgt ctttggaagg cctgtattgg acttgcagaa atttttgaag ccctagaaca
    22381 actgagtctt agcaagcgat acaaaaacat tgcctgtaaa ctaaaggaat caatttgtaa
    22441 acaaatgact atcgatggtc cttttggtga gcaatttttg gagggaatcg gtgatgagga
    22501 gaaaacaacc tattcagtga agcactatca aaaaccgatt cttgagcaag ggttaatctt
    22561 tttaagtgat gtgattgtgg atggaaaaat taatttattg atgcatgatg gagaagaatc
    22621 agacactacg ttgatgcctt tttatcaatt tttagataat aaagatcatt tatatcagaa
    22681 caccatggcc ttttctgcta gttctttcaa tccaacctat agcgaggaaa tcaaaggcat
    22741 cacttgggga ttagaatccg gcgccacttt tcctggcttt atcactgttt taatgagcga
    22801 gctgcacaac cctcaagctt tcgtaacccg attagaagaa ctaatttgtt tggcagattt
    22861 agacggttcg tggtggtggt ggccgtatcg cttagaggct caacaaggag aagtcgtgcg
    22921 agactttggc tgcggcaaat gcggctgggc atcgggtctt tttgtcagcc taatgatcag
    22981 tcaatatttc ggattgaaat tcaaaaaagg agtattgcaa attgatcctg ttgataattt
    23041 aacgttttct tggaagaatt tgaagttcgg tcaagccttc atttcgattg aatgtacgca
    23101 atccgagttg agtatttcaa atttgtcaga aaagccattg aaaataagtg atacgaaaaa
    23161 aattcttcac gtagaaaagg gaaaaacgat tcacattcaa aggagaaaaa gatgagtata
    23221 ctaaaaaata ttacccaatt taaaaaaatc cagagtcgag caatcactcc cgaaaatcca
    23281 acaggcgaga aaggtaaagc tgcgatgttg ccaagcgagc taggaccaag ccgcaaaggt
    23341 cgtggcgcaa ttccgctgac ttctggtgaa gaaactgtga ttgcggatat tactggaacg
    23401 ggaataattc gtcatatgtg gatgacgatt cgtgatcgaa cagaatttgg cagttttgtt
    23461 ttacgtgatt taatcttaag aatttattgg gatgggtcgg atagccctgc tgttgaaagt
    23521 cctttagggg atttcttttg taatgggttt ggagagcgtt gcgaggtgaa ctcgttgccg
    23581 atagtggtta atcctacagg gggctttaat tcctatttcg aaatgccttt ccaaacatcc
    23641 gctaaaatta cgatcgagaa tttacatccg aaggatatgt cctcattttt ttatacgata
    23701 aattacgctt tggtggacga attggaagaa aatagcctat attttcacgc aaattggcat
    23761 cgggaacgca ttaccaaatt acaggaggat tataagttaa ttgatcgatt aaagggtcaa
    23821 ggctattacg tgggtactta ccttgcgctt acctgcctag aacgttattg gtggggcgaa
    23881 ggcgagttta aattctacat tgatggggat agtgagtatc cgaccgtaac ctcaacaggt
    23941 tcagaagatt attttggcgg ggcgtgggcc tttcatgagt acgatgagtt cggacgtcca
    24001 catgcgaaaa actattcaac agccttttta gggtatcctt accaatcaaa tagagatgcc
    24061 acaagagatc gttttgaaac gggaaaattg aatgccgttc atgcctttgg tgacgacgca
    24121 atgccacgac atggtcttta tcgttggcat cttcaagacc ccatctgttt taccgaagat
    24181 ttatcggtca ctttacaaca aatcggcaac gatgatgttc gcttatttga gcggcaagac
    24241 gatgttgcta gtgtcgctta ctggtattca acagaagctt caggaaatac taaacgtcat
    24301 ttcactgatc gagaacgctt gccgcgttaa tgaagtgttt ttcacatatt cgaagggagg
    24361 aacctatgat gatatatggg gcaattgaag ctggagggac taaatttgtc tgtgcagtcg
    24421 gtactgagga attaacgatt ttggaaagag tcagttttcc cacaacaact ccagttgaaa
    24481 caatgaagca ggtcattgat ttttttaaac cttttaagga acaattggca ggcatcgggg
    24541 tcggctgctt tggtccaatt gatattcaat gcaaatcaac gagtttcggt catatcacat
    24601 caacaccaaa acttgcttgg caaaatttca attttgtagg aacgctaaag caatatttca
    24661 atattccgat tgaatggaca acagatgtca atgcggcttg ctatggtgaa tatgttgctg
    24721 gttctggcaa agggttagca ggtgttgcat acttcactgt gggaacaggt atcggaggtg
    24781 gtgccttgtt caatgggact ttcgtggaag ggttcagtca tccggaaatg gggcatatgt
    24841 tgatcaagcg acatccaaag gacaattttt caggaaactg tcccttccac caagattgtt
    24901 tagaggggat ggcagcagga ccagcgattg aaaaacggtt gggtgtaaaa ggccagaatt
    24961 tattggctga cgattcgttt tggcagattg aagcctttta ccttgcccaa tgtgcataca
    25021 atacaacact gatgttttcg ccagatagaa ttatttttgg cggtggtgta atgaaacaag
    25081 aacacatgaa gaaaaaagtt caggataaat ttgttgagct tataaatgga tatgttgaaa
    25141 taccaccaat tgatagctac attattacgc ccgaattagg tgataatgcc ggaataatcg
    25201 gcggattagc attggccaga aaagcggtaa gaaataagca accataatta atgaatgttt
    25261 cattgatact catgtcaaaa gtatttaacc gagtaatcaa atgatcaaat gaagttggat
    25321 aaagcgaaaa tgaattcaaa taaaaaggct ctaagtcaaa tcgtgttgtt ttcattaaat
    25381 cactgaaagg aaatgcttct gcgtttcctt ttttcttttt gttgtgcgct gtataccttg
    25441 aggatattca ctggtggcgt acacttaaat agtaatatat tgtgttactt tatgtctgtg
    25501 gtaatagtta tagttgctta ccaatgtatg ttcatttttc gaaatgttga cttcataatc
    25561 cttggcttaa tgtttgaatt tttttggtga tagatttttt cagcc
//
LOCUS       DACDRK010000035        24702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDRK010000035 DACDRK010000000
VERSION     DACDRK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.035"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWJ77_001535"
     CDS             949..1422
                     /locus_tag="IWJ77_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9453143.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWJ77_001536"
     CDS             1431..1658
                     /locus_tag="IWJ77_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9453144.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IWJ77_001537"
     CDS             2294..2665
                     /locus_tag="IWJ77_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453145.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IWJ77_001538"
     CDS             complement(2921..3163)
                     /locus_tag="IWJ77_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9453146.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IWJ77_001539"
     CDS             complement(3195..4097)
                     /locus_tag="IWJ77_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9453147.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IWJ77_001540"
     CDS             complement(4110..4298)
                     /locus_tag="IWJ77_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9453148.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWJ77_001541"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWJ77_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9453149.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IWJ77_001542"
     CDS             complement(4913..5806)
                     /locus_tag="IWJ77_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9453150.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6822)
                     /locus_tag="IWJ77_001543"
     CDS             complement(5884..6822)
                     /locus_tag="IWJ77_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9453151.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="IWJ77_001544"
     CDS             complement(6856..7206)
                     /locus_tag="IWJ77_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9453152.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="IWJ77_001545"
     CDS             complement(7239..8141)
                     /locus_tag="IWJ77_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9453153.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="IWJ77_001546"
     CDS             complement(8134..8991)
                     /locus_tag="IWJ77_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9453154.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="IWJ77_001547"
     CDS             9325..10134
                     /locus_tag="IWJ77_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9453155.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="IWJ77_001548"
     CDS             10174..10671
                     /locus_tag="IWJ77_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9453156.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="IWJ77_001549"
     tRNA            complement(11056..11129)
                     /locus_tag="IWJ77_001549"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="IWJ77_001550"
     CDS             11316..11639
                     /locus_tag="IWJ77_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453157.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="IWJ77_001551"
     CDS             11803..12057
                     /locus_tag="IWJ77_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453158.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="IWJ77_001552"
     CDS             12127..12372
                     /locus_tag="IWJ77_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9453159.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="IWJ77_001553"
     CDS             12448..12813
                     /locus_tag="IWJ77_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453160.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="IWJ77_001554"
     CDS             complement(12874..13437)
                     /locus_tag="IWJ77_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453161.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(14005..14205)
                     /locus_tag="IWJ77_001555"
     CDS             complement(14005..14205)
                     /locus_tag="IWJ77_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9453162.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(14957..15670)
                     /locus_tag="IWJ77_001556"
     CDS             complement(14957..15670)
                     /locus_tag="IWJ77_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9453163.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15663..16748)
                     /gene="uxuA"
                     /locus_tag="IWJ77_001557"
     CDS             complement(15663..16748)
                     /gene="uxuA"
                     /locus_tag="IWJ77_001557"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9453164.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(16765..17208)
                     /locus_tag="IWJ77_001558"
     CDS             complement(16765..17208)
                     /locus_tag="IWJ77_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9453165.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(17242..17595)
                     /locus_tag="IWJ77_001559"
     CDS             complement(17242..17595)
                     /locus_tag="IWJ77_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453166.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(17707..18108)
                     /locus_tag="IWJ77_001560"
     CDS             complement(17707..18108)
                     /locus_tag="IWJ77_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453167.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(18145..18654)
                     /locus_tag="IWJ77_001561"
     CDS             complement(18145..18654)
                     /locus_tag="IWJ77_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9453168.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(18676..19533)
                     /locus_tag="IWJ77_001562"
     CDS             complement(18676..19533)
                     /locus_tag="IWJ77_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9453169.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(19551..20384)
                     /locus_tag="IWJ77_001563"
     CDS             complement(19551..20384)
                     /locus_tag="IWJ77_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9453170.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(20398..21195)
                     /locus_tag="IWJ77_001564"
     CDS             complement(20398..21195)
                     /locus_tag="IWJ77_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9453171.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(21228..21512)
                     /locus_tag="IWJ77_001565"
     CDS             complement(21228..21512)
                     /locus_tag="IWJ77_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9453172.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(21509..22510)
                     /locus_tag="IWJ77_001566"
     CDS             complement(21509..22510)
                     /locus_tag="IWJ77_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9453173.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(22512..23414)
                     /gene="gnd"
                     /locus_tag="IWJ77_001567"
     CDS             complement(22512..23414)
                     /gene="gnd"
                     /locus_tag="IWJ77_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9453174.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            23578..24426
                     /locus_tag="IWJ77_001568"
     CDS             23578..24426
                     /locus_tag="IWJ77_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9453175.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         7225 a         4898 c         4000 g         8579 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atgaattttt tcgtacaaaa aaaccgtctt ttctttcgaa
    13981 aagacggtta aaagtcaatt tttattaagc tttaacaatg tttgttgctt gagggccacg
    14041 ttgaccatct tcaacatcgt aagacactgc ttggccttct tctaaagttt tgaagccgtc
    14101 accttggata gctgaaaaat gtgcaaatac atcatttcca tcttcaccag tgataaagcc
    14161 aaaacctttg tctgcgttga accattttac tgtaccgtta ttcataaata aattcctcct
    14221 gatacgtatc aatgtacgtt ttttcgttgc aataaatatt tgataggcaa aaggagtttt
    14281 atctatgtag atatatcgct caaattacgt ttcaaaatag attactcatt taatatatca
    14341 tgctttttct aataacacaa ggataatgca atgaaatata aaattcattg tcttctattg
    14401 tcttcgaatc gaagaaaaag ttgaataacc gcgattgatt cactttttgt tttcttgttt
    14461 atttgctttt atctgcaact tttgacttta aaatttctta actatgaaaa cctttcaatt
    14521 ggtggtatac ttataaatgg aatttcgtta tcaacgcgtt cgttgccttt cagcaagtat
    14581 ttaaagaaag gagaatggct atgacattct ataacaaaga tatacaatat cgagtaacct
    14641 tggatacaga tttgaatcta tttgtcgttt ttgacaaaga aaataccaat catgtagcga
    14701 caggtgttac gatcgaacaa gcagtgcaag aattaaaaaa atctgcataa ggagtggaaa
    14761 ccctcatatg gacgattctc gttcttctct ttaaacgcat ctactggtag ataacagtag
    14821 atgcgttttg tgtttgttct aaattgttca ctacaaataa ggtagatact tcaaaataaa
    14881 aaattacttc tttttcatct gttccctcag tcgtgcagga caataaataa agaggactag
    14941 aaaatagtcc tctttattag agatacaacc ctttactttc taaaaatcgt tttgcttgat
    15001 gcaggtcata aaattcatag tctgctttat tggattcata taaacttaca tctactttca
    15061 acttatgctt cccattatga tcgggcaacc ctatcaaatg atctttgatg agaaaagaca
    15121 aaatgcccgc tcgccttgct gcttcgatcc ctgcaatcga gtcttcaaat acaagtgctt
    15181 cacttggagg aatattcatt tttgaacaag ctttcaaaaa gatctctggt tccggctttc
    15241 ctttttttac atcatcccct gaaatgattt gatcaaacaa ggcatataga ttttccattt
    15301 ttaaatacat ttctactttt acccgtgtat tagacgaagc cacgattgta tggattcctc
    15361 tttcttttgc aaaagataaa atttcttctg ctcctttttt cgtatatact ctcttatctt
    15421 ctttcacgat tttttcttta tattgattga ttgcttctcg ccatttgata tatggaacat
    15481 cttctccgta caaaatgccc atttgttctc ttatcgccat ttctgtttgg ccagttagta
    15541 aataatagac ttccttacgc atttcataat catacttttg tgctgatttt acataagcac
    15601 gataagctaa acgtccagtt tctaataaaa ggccatccat atcaaagatg aagagcttga
    15661 ttttattcat aatgctctcc cagtggtttt tccttactta tcgcctcata tagaccttgc
    15721 atgtaagtga gtcccattgc acgatcatac aggccatatc caggcatagc cacttcaccc
    15781 cacacggttc taccatgatc agggcgtata ggaccgtcga acccttcttg caccaacgct
    15841 ttcataatat gatacatatc aagggagcct tctgtagagg gatgtgccgt ttctttgaac
    15901 tttctttctc ctaaaaactc cacgtttctg aagtggacaa aatggatgcg cgaacctacc
    15961 tcatggatga tttcaactaa atcgttttcc ggatttgccc ctaaagatcc tgtgcacagc
    16021 gttagtccat tatatggaga atctactgca tttagaattt tcttcaagtc gccaagattt
    16081 ttcgtcactc taggaaaacc aaaaagttcc catggcggat catctggatg gatagccatt
    16141 tttacatctg ctttttcgca cactggaatc actttttcta aaaaataagt cagattttca
    16201 aataattctt cttcggtcat tccttcatac agtttttcca gctgagcgaa ttttttcaac
    16261 cgttcttctt cccaacccgg caatttgaat ccctttgact ggctgtgtat caattgatac
    16321 atttctcctg gctccatctg atcgacgatt ttctgatcgt ataaaagaga atagcttcca
    16381 tcgttgtttt cattaaacaa atcagttttc gcccatccaa aaattggttt gaagctataa
    16441 caaatcaatc gaaccccaca tcgagacaag tttttgattg ttcgtatata gttatcaata
    16501 tagtaatctc gttcatctgt ccctgccttg attgcatcgt ggatcgctac actttcaatt
    16561 ccaagcagtt ccagtccttg gctctcaaca gatgctttca actcctcaat ttctgaaatc
    16621 tcccaaacat ctccaggcat tttagtcagc aaagttccaa ttatcccatt tattcccggt
    16681 atttggcgaa catattttaa tgggatcgaa tccccttttt caccatacca tctaaatccc
    16741 cacttcatct tttcagcctt ccttctaaaa atcgtcctca tctaattttt tttcttccgc
    16801 tgaagcatgc caaacagtta aattatcttt tccttgtttg acaatttgtt ccaccgcttc
    16861 ttctatcggt gtgtgaagca tctgatgatt gattgcttct aacaacattg gcagattcac
    16921 gccccctatg acataagtgg ctgctttctt ttcttcagtc agttcattga ctgccaagag
    16981 cgcttgattg tacggacttg cgctaattag atcaacaaat actagcatgg gttctgctgt
    17041 gttgatcata gttaattttt ctttgacctt ctctccgaat gtttggatgt cctccccaga
    17101 ctctaagcac attgtttcaa tatggctcga tgtcccgaca attacttccg ccgcatcttt
    17161 cagtccttcg ctcaacttac catgtgtaac gatcatgatt tccaacatta gtcttctcac
    17221 tccttgtttt gcacttttta cttatcgaaa aagagcaata atctgattgg cattttgcca
    17281 attaaatgtt ttccctcgta aatctgtttt ggtcaaatag aagaactgcg cttgcttttt
    17341 tcttgcacta aaaatataat tgacgttttt ttctttcaac ttatcaactt gtttcagcca
    17401 ctgttcttta tttgaaaaat aatttcccga aaactgtagc ggtgtatctg ccccaacaga
    17461 gtctagataa aaaagaatag cctctttttt catacccgat ctcacgctgt ctaagccctt
    17521 ttctccataa cagccttcat caacaaacgc atacgctaca ctcggatcat caatcgtacg
    17581 gatcatttct agcatcgtta atatggatga agtatttcga ataaaagttt gtctgctagg
    17641 ccatccgcga gctgcttttc ccaataagaa aaagtagctc ccaaatgcta gtacagtgat
    17701 acctgtttat acggatagca gatcgaatac gtgatgagga tttttgttat atatcatcat
    17761 tccccaccaa cctaaactga acaacaaaat gaaggacaat aatatagagt agattgtttt
    17821 tttcctttgg tctttccaat caaacatgaa ataacttcct aatgcatgaa cgggtgtatc
    17881 gtaataagta cagataactt tctctgcttt ttcgatatct ccaacataaa tattttttga
    17941 tcggtaggct agcgtatcgt aagcaatgac tgtcacatct gttctcatac tgtaaatatc
    18001 tgatactaat gctgaaagaa aacgctgttt actctttttt gtacggcgct tacggtaaat
    18061 atagcgataa cgaaattccc aatcacgaaa cagctcgttc ttcttcattt tttcgccctc
    18121 tttatctttt ttctaagcat ttgtttatga caaatcaaac ttgttcaata catcttttaa
    18181 tcctgtttta ggtgcagatg gagtggcttg aaaataaata ttctttacgc catattgttc
    18241 attcatttct ttcaattttt cagcatctga tttatttaaa taaacagatt tcgtcactaa
    18301 aagatctgta tcaggatttt tttgagcaat ccctccaata ttcaattctt ccatggggat
    18361 tccgtgtttt actaagtcgt aaatcgtttg gactgttttt gcgatcaaaa tacaattgta
    18421 ttctttaaat tgatattcat cccaatagaa tttagctttc tctggtgtaa tgacaattgt
    18481 tttttgtcct gtacggcttc ctgcattttt gtaaagatct ctcataaatt tatcttttgc
    18541 tacaacttcg tctacaacaa aaatggaatt cactccactt ttttgagata acgttaccat
    18601 tacttgccca tggatcaaac gctcatccac acgcgctaaa ttgactacac ccatcttatt
    18661 cacgttccct tctttttata aaataccgat tcctgctagc acaattaata ttccagtcag
    18721 ccataataat gcttgtgtga ctttcaatcc ttttttcgta tagaacaagt acacacccat
    18781 tacgacagct aaaggtaata tccctggcac gatttgatca agaatttcct ggataacaaa
    18841 ctcacgtccg gatatcgcaa atttcaatga agattctact tttacatagt ttcccgcaag
    18901 cacccccatc ataaacaacc ctaaaactga tagcatttca atcgctgtct gaacaccagt
    18961 gttttgcttt aggcctgtag cattacggcc catagtgaaa gcttgtcttg cgaaaaatac
    19021 ccctagtaaa acctgataca acccaaagca gataaaagga aaaagtgcgc ctaaaggact
    19081 tccctgttgt gcccaaggta ccgcgattgc gataaaaata tattggacgg ttcctgaatc
    19141 gattgcatct ccgattcctg ctagtgcccc catcaatccc gctttcgtgt tatacatcaa
    19201 gtcatcctgc atcaagatct ctttttcttc atactcttct tctgctcttt gctgttccat
    19261 agaagacatt aggccagtaa tcacaccgcc accccagctt aattgagtgt tgaagaacag
    19321 taactgccgt ttatatgctg cttttaaaga gtcttcatct ttataaagtt ttttcaagat
    19381 tggcatcatt ccgtatagta gtgatggcgc caagtaacgt tcaaaagagt gaggaatttc
    19441 gtttgcaaaa tgccatctca gataggcttt tgttacatct tttttcgtga tttcttcagg
    19501 agcacctgtt tgattatcca ttatttcagc catttcccat tcctcctaaa ttaaaatccg
    19561 tcatcatagt catcttcgtc atcaaaagat gctgaattgt tattgcttgt ggcaggctgt
    19621 acttgtttcc ctttttgcga ctggacaaag atcaaagcga taataatccc aaaaatggca
    19681 taagtaacca tcgtaatatc taaagatttc aaaacaacac tcataaaata agcaaggaag
    19741 aaaaagacca gataatcttt tcgaccgata acgtatacag ttgtcgcgat accgattgcc
    19801 gctaaccctc cacctactac ttcaagaatg tggataacga ctgttccctc taaagctttg
    19861 attacatcag cgatcaatgg cgcccctaaa tataaagcaa tgaaaacaag cggaacgaat
    19921 aataaaaatg aggcaaccgt tggataaaga ataattgatc gggccatagc ttttgtattc
    19981 aaatcttcta cagctttgtc tgtatattta cctaaaaaag tattgatgaa aaaacgaaac
    20041 tgatacaagt aacttccgag caatccgact ggcaccgcaa cggcaatcgc tgcttctggc
    20101 gacagatccc ctaataatgc gactggtact gcgatagctg ctgcaataga tggttcagct
    20161 ggcattgatc ctcctggaga aaaaaccccc atataaatca tttgcaaagc ggctgtaaca
    20221 atcatcgctt gtgcaacttc ccccatgatc aacccgacga ctaatccagt catcaaagga
    20281 gaaaaacgta aggtcaacgt ggctcctcca cctaaccagc gggacataac cgcggctacc
    20341 cataaagcaa tcaacaaact ttgtactaca cttaatgttt ccatgtgtcc tcctccttca
    20401 gatctttctg atataacctt ccagttccat cattgactgt atcagtgatt tctgtgttac
    20461 ctctatagga atcaaatgca ctttctcttt cgaatcaata aattcagcca ttttatctat
    20521 tatctgttta tctttcggaa ataatcccat ttgttctaga gaaaccggca gttctaattc
    20581 ccgatacaaa ggatataact cgtctatttt ttcccagcga ccttctagtg ccagttgata
    20641 gaaaatccca tacgctactt tttctccatg tagaaaacga tggctttccg gaatatattt
    20701 actgatagcg tcatgcatag catgtgctgc cacatttctt gcgtatttat ctcctaatcc
    20761 tccaactaaa ccagcaacag caaagataat ctctgaaagc tgtttgaact ccgatgtcag
    20821 ttttctttgt gccatatctt ggattgcttt agcagaatga ttcaaaatct cttctttgca
    20881 gatcttcgca gtatcttgtg ctagcttcaa aaatgattca tcttgcaaaa aatcttgttc
    20941 taagatgagc tcagattcat accattttgc tagtgtatct gctaacccgg caatgaaata
    21001 tggaacaggg gaatcaatca ctaattgggg atctgttata aaaaaagcag cttgtctgta
    21061 aaagtgttct gttttccctt ctgcttgtcc attctcctta tacatgacag caagaggtgt
    21121 ccatggagca caattactag ctaaagtagg gatgactccg aaatcgattc ttgcaagatt
    21181 ggcactataa cccactagat cggtcagttt tcctccgcca acgccaacta cgaaatcgat
    21241 ttcatttttt tgatgattgt agctattcgt ctggcttcag cataactaca ttctccagaa
    21301 taacgatgat aaaaaaattg aatcgtttcg tcattcaaaa atactaattt aggtagcgcc
    21361 ttttcccatg ataccgttcc gtgggtgacc agtactcttt tcgcgccgta tgatttcaag
    21421 agatcagaaa cttttgaaat accaccttct tcatataaat aaaattgagg gcctgttttc
    21481 acttttaaac cgttcaccat tttcttcatc atccttttat atagttgctc tttgtctgga
    21541 cagaacgttc aggaagaagg tgctgactga tctgttcgac atctctgaca catttttcac
    21601 ccattgcttc taaacattcc cttgtgtagg ccgatatatg tggcgtaatc actacttgtt
    21661 caaacgctaa atacggatga tcttgacgtc ccggttcttc ttctaaaaca tctgtggcat
    21721 atcctgcgat ctttccgctt ttcagaccat taatcatcgc ttcttcatca actagagcgc
    21781 ctcttgcact attagataga taaacatttt ctttcatctg atcgacttct ttatcagaaa
    21841 tcatgtgata gttctcttct gtcaaattcg cacataaaca aatgatatct gattgttcca
    21901 gtaattcttg aagttctact ttttttgcac caaagctttc gatgtataaa tctgatttgt
    21961 aaggatcata agctagaaca tcacagcgaa aaccgttacg caaaatttcg attactccgc
    22021 ttccggtatt tcctgcacca atcacaccaa ctgttttgtt aaacagcgtc ctaccgacga
    22081 actccgcccg atcttcccat cgatcgtcct tgactcttgc ttgtgcttgt gtcgtgcgtc
    22141 gaagcatgga caacaaatta gttatattat tttctgctac agcatttcgt tcaactaaag
    22201 caggtatgat agatacgatt gtctggtgtt cttttgctgc ttttaaatca atattgttgt
    22261 aaccaatacc atgtcgggaa atcaatagca gttcatcttt gttttcaaaa aaatctcgtc
    22321 gaaaaaatgg cgtaacactt gcaataatga tattgaatcc tttaagtaac tccgctagtt
    22381 cttttcctgg aatctcttga tcaacagtaa agaaatgaac gtctccaact gactttaatc
    22441 tttccaagtg gtgtggaaag actcttccga aactgctgga attaacgatt gctatatgat
    22501 aatctggcat tttactcacc cttttcaact atttcatgtc cgccaaaccc atttcttagt
    22561 gctgaaacta cttttgctga aaaactatct tcttcctgtg aaagattcct catgaacaaa
    22621 ctagttgcaa ttactggaac tggaacagcc atttctagcg ctgtttcaac cgtccatttt
    22681 gcttcacctg atgcagaaac gattccccga taattactta aattgggatg ttcgtttaac
    22741 tgatcttcca tgatttccat cagccagccg cgaatcaccg aaccatgatt ccaaacacgt
    22801 gcgacttgtt ccaagtcaaa agcatactca cttttttgta caagttggaa tccttcaccg
    22861 attgcttgca tcattccata ttcgattcca ttatggatca ttttcaagaa gtgtccgctt
    22921 ccggatgcac cagcatacag atatcctctt tcaattgcta catctttgaa taattgttcc
    22981 acggatagaa aagtttcttt atctcctcca atcattaagc aggcttgatg tcgtgctcct
    23041 tcgattcctc cagaagtccc acaatccaag taaccgattt catttttctg aaactcttct
    23101 gctcgacgga ttgaatcttt ataataagag tttccgcctt caataataat gtctcctggt
    23161 ccgagttttg gaaaaagttt tcttaacatg ttttcggtta tctctccagc aggaagcatt
    23221 aaccagatga tttttttctg ctcaaaagta gctagaaatt cagagatctc ttgataagca
    23281 ggaatgtcat tttctcttgc ctgactaaca gctgtacttg aaatgtcgag tccttgtact
    23341 ttgtgatggt gctcttttaa gtttaaagct aaattcaatc ccatttttcc caaaccaatt
    23401 acaccaattt ccatgatagc gcctccattt cttataccct cattatacaa aaaaattttt
    23461 ttgtaaatag attttacaaa aatatttttt gtttgatttt ttcctactct ccttgtatct
    23521 ttaacattaa tgagaacgga aaaaatgata atcttattat gaaaatgagg aattctcatg
    23581 agcgtctcca taagactaca aatcaaaaca atgtacaacg acttcagccc aaaagaacag
    23641 gctatcgccg attatatttt agaaaatcca agcaaagtaa gtcatagtcc tattagcaat
    23701 ttagcaagtg aactgggcat tgctgactct actttttttc aatttacaaa aaaacttggt
    23761 tataatggat ttaaagattt taaaatggcc atgttgatcc aagaaaatga tctatctgct
    23821 atttctatcc acgaaaatat tcagaaaaca gatactgagc tcactatggc gcaaaaggtg
    23881 ttcgagtcta gtatttcaac tttaaatgac acaaaaaagt tgttacaaca aaaggatcta
    23941 aaaaaagcgg ctactattat caatgaatct aaacgtgtct attttttcgg tgttggcggt
    24001 tcagagattg tagcaactga cgcttatcat aaatttttgc gttcccctat cgcaacttct
    24061 cacagcacgg attaccatat tcaattgatg gaagcttctt tattgacgga aaaagactgt
    24121 gctgttttga tttctcacac agggcaatca aaagaaacaa tccatattgc tgaaaccgta
    24181 agaaaaactg gagcaaaaac aatcgttatt actagccaag ctaattcttc ccttgctaaa
    24241 cttggcgatg tcgtttttat ttctatttct gaagaaacag aattccgatc tgaagcttta
    24301 gcttcccgta tttctcaact aagcattttg gactctctat atgtgatttt gatgttttac
    24361 aacgaaaaag aatcacgtcg ttctatcgct aaagttcgac atactatttc tgaaatcaaa
    24421 gaatagcacc ataacagaaa aagcgaaaaa tccataaaac aaaaaaagcc actaccaaat
    24481 atttggtagt ggcttttttg gttctgcaag ggtggtacag aacatcaata gtcggtgggt
    24541 tagaacgtat gtcatcgcat ctttggagaa ggttgtattg gtattattcg agttacttgt
    24601 tagatgcgat aacaaagtta gtatacagta gtaaaaaatt tatcttttct tttttttatt
    24661 atttttgttt cttattttct aaaaaattaa aaaaaatttt gg
//
LOCUS       DACDRK010000036        23831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDRK010000036 DACDRK010000000
VERSION     DACDRK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.036"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IWJ77_001569"
     CDS             complement(55..789)
                     /locus_tag="IWJ77_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9453176.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IWJ77_001570"
     CDS             1116..1526
                     /locus_tag="IWJ77_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9453177.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IWJ77_001571"
     CDS             1519..2220
                     /locus_tag="IWJ77_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9453178.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IWJ77_001572"
     CDS             2220..3806
                     /locus_tag="IWJ77_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9453179.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IWJ77_001573"
     CDS             3825..4046
                     /locus_tag="IWJ77_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9453180.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IWJ77_001574"
     CDS             4043..4804
                     /locus_tag="IWJ77_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9453181.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IWJ77_001575"
     CDS             5184..5693
                     /locus_tag="IWJ77_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9453182.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IWJ77_001576"
     CDS             complement(5768..6307)
                     /locus_tag="IWJ77_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9453183.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWJ77_001577"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWJ77_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9453184.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IWJ77_001578"
     CDS             7374..8171
                     /locus_tag="IWJ77_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9453185.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IWJ77_001579"
     CDS             8199..8834
                     /locus_tag="IWJ77_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9453186.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IWJ77_001580"
     CDS             8854..9627
                     /locus_tag="IWJ77_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9453187.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IWJ77_001581"
     CDS             10035..10832
                     /locus_tag="IWJ77_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9453188.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IWJ77_001582"
     CDS             10810..11223
                     /locus_tag="IWJ77_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453189.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IWJ77_001583"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IWJ77_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9453190.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IWJ77_001584"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IWJ77_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9453191.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IWJ77_001585"
     CDS             16000..16560
                     /locus_tag="IWJ77_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9453192.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWJ77_001586"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWJ77_001586"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9453193.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IWJ77_001587"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IWJ77_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9453194.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IWJ77_001588"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IWJ77_001588"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9453195.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IWJ77_001589"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IWJ77_001589"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9453196.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IWJ77_001590"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IWJ77_001590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9453197.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IWJ77_001591"
     CDS             21907..22485
                     /locus_tag="IWJ77_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9453198.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IWJ77_001592"
     CDS             22537..23019
                     /locus_tag="IWJ77_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9453199.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IWJ77_001593"
     CDS             23136..23510
                     /locus_tag="IWJ77_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453200.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DACDRK010000037        23065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDRK010000037 DACDRK010000000
VERSION     DACDRK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.037"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IWJ77_001594"
     CDS             complement(109..564)
                     /locus_tag="IWJ77_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453201.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IWJ77_001595"
     CDS             complement(718..1926)
                     /locus_tag="IWJ77_001595"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9453202.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IWJ77_001596"
     CDS             complement(2038..2370)
                     /locus_tag="IWJ77_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9453203.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IWJ77_001597"
     CDS             2518..3393
                     /locus_tag="IWJ77_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9453204.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IWJ77_001598"
     CDS             complement(3475..4056)
                     /locus_tag="IWJ77_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9453205.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IWJ77_001599"
     CDS             complement(4062..5321)
                     /locus_tag="IWJ77_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9453206.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IWJ77_001600"
     CDS             complement(5323..6369)
                     /locus_tag="IWJ77_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453207.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IWJ77_001601"
     CDS             complement(6604..6879)
                     /locus_tag="IWJ77_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9453208.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IWJ77_001602"
     CDS             complement(7122..8432)
                     /locus_tag="IWJ77_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9453209.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWJ77_001603"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWJ77_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9453210.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IWJ77_001604"
     CDS             complement(9978..10658)
                     /locus_tag="IWJ77_001604"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9453211.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IWJ77_001605"
     CDS             complement(10707..11342)
                     /locus_tag="IWJ77_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9453212.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IWJ77_001606"
     CDS             complement(11408..12811)
                     /locus_tag="IWJ77_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9453213.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IWJ77_001607"
     CDS             complement(12798..13829)
                     /locus_tag="IWJ77_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453214.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IWJ77_001608"
     CDS             13860..14081
                     /locus_tag="IWJ77_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9453215.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IWJ77_001609"
     CDS             complement(14246..14983)
                     /locus_tag="IWJ77_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9453216.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IWJ77_001610"
     CDS             complement(15136..16476)
                     /locus_tag="IWJ77_001610"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9453217.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IWJ77_001611"
     CDS             complement(16713..18236)
                     /locus_tag="IWJ77_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9453218.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IWJ77_001612"
     CDS             complement(18614..19225)
                     /locus_tag="IWJ77_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9453219.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IWJ77_001613"
     CDS             complement(19555..20271)
                     /locus_tag="IWJ77_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9453220.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWJ77_001614"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWJ77_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9453221.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IWJ77_001615"
     CDS             complement(20830..21627)
                     /locus_tag="IWJ77_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9453222.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IWJ77_001616"
     CDS             complement(21597..21968)
                     /locus_tag="IWJ77_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9453223.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWJ77_001617"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWJ77_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9453224.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaa
//
LOCUS       DACDRK010000038        22028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDRK010000038 DACDRK010000000
VERSION     DACDRK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.038"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..1371)
                     /locus_tag="IWJ77_001618"
     CDS             complement(61..1371)
                     /locus_tag="IWJ77_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9453225.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IWJ77_001619"
     CDS             complement(1383..2801)
                     /locus_tag="IWJ77_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9453226.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IWJ77_001620"
     CDS             complement(3041..4609)
                     /locus_tag="IWJ77_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9453227.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IWJ77_001621"
     CDS             complement(4613..4894)
                     /locus_tag="IWJ77_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9453228.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IWJ77_001622"
     CDS             complement(4891..5814)
                     /locus_tag="IWJ77_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9453229.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IWJ77_001623"
     CDS             complement(5811..6689)
                     /locus_tag="IWJ77_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9453230.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IWJ77_001624"
     CDS             complement(6676..8013)
                     /locus_tag="IWJ77_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9453231.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IWJ77_001625"
     CDS             complement(8016..9119)
                     /locus_tag="IWJ77_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9453232.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IWJ77_001626"
     CDS             complement(9193..10701)
                     /locus_tag="IWJ77_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9453233.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IWJ77_001627"
     CDS             complement(10667..11359)
                     /locus_tag="IWJ77_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9453234.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IWJ77_001628"
     CDS             complement(11381..12355)
                     /locus_tag="IWJ77_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9453235.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IWJ77_001629"
     CDS             12655..13527
                     /locus_tag="IWJ77_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9453236.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IWJ77_001630"
     CDS             complement(14109..16307)
                     /locus_tag="IWJ77_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9453237.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IWJ77_001631"
     CDS             16539..17531
                     /locus_tag="IWJ77_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9453238.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IWJ77_001632"
     CDS             17591..19012
                     /locus_tag="IWJ77_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9453239.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IWJ77_001633"
     CDS             19005..20504
                     /locus_tag="IWJ77_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9453240.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IWJ77_001634"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IWJ77_001634"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9453241.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DACDRK010000039        21913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDRK010000039 DACDRK010000000
VERSION     DACDRK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.039"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="IWJ77_001635"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="IWJ77_001635"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9453242.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="IWJ77_001636"
     CDS             complement(1173..1868)
                     /locus_tag="IWJ77_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9453243.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="IWJ77_001637"
     CDS             complement(1881..2597)
                     /locus_tag="IWJ77_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9453244.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3535
                     /locus_tag="IWJ77_001638"
     CDS             2927..3535
                     /locus_tag="IWJ77_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9453245.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3860..4183
                     /locus_tag="IWJ77_001639"
     CDS             3860..4183
                     /locus_tag="IWJ77_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9453246.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4365..5735
                     /locus_tag="IWJ77_001640"
     CDS             4365..5735
                     /locus_tag="IWJ77_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9453247.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5865..7166)
                     /locus_tag="IWJ77_001641"
     CDS             complement(5865..7166)
                     /locus_tag="IWJ77_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9453248.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7520..9511)
                     /locus_tag="IWJ77_001642"
     CDS             complement(7520..9511)
                     /locus_tag="IWJ77_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9453249.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9655..9921)
                     /locus_tag="IWJ77_001643"
     CDS             complement(9655..9921)
                     /locus_tag="IWJ77_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9453250.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10431..12557)
                     /gene="pnp"
                     /locus_tag="IWJ77_001644"
     CDS             complement(10431..12557)
                     /gene="pnp"
                     /locus_tag="IWJ77_001644"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9453251.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12745..13014)
                     /gene="rpsO"
                     /locus_tag="IWJ77_001645"
     CDS             complement(12745..13014)
                     /gene="rpsO"
                     /locus_tag="IWJ77_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9453252.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13265..13828
                     /locus_tag="IWJ77_001646"
     CDS             13265..13828
                     /locus_tag="IWJ77_001646"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9453253.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14081..14602)
                     /locus_tag="IWJ77_001647"
     CDS             complement(14081..14602)
                     /locus_tag="IWJ77_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9453254.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14605..15447)
                     /locus_tag="IWJ77_001648"
     CDS             complement(14605..15447)
                     /locus_tag="IWJ77_001648"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9453255.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15440..16165)
                     /locus_tag="IWJ77_001649"
     CDS             complement(15440..16165)
                     /locus_tag="IWJ77_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9453256.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16341..16637
                     /locus_tag="IWJ77_001650"
     CDS             16341..16637
                     /locus_tag="IWJ77_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453257.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17205..18797
                     /locus_tag="IWJ77_001651"
     CDS             17205..18797
                     /locus_tag="IWJ77_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9453258.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18810..19550
                     /locus_tag="IWJ77_001652"
     CDS             18810..19550
                     /locus_tag="IWJ77_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9453259.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19718..21067
                     /locus_tag="IWJ77_001653"
     CDS             19718..21067
                     /locus_tag="IWJ77_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9453260.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21195..21785)
                     /locus_tag="IWJ77_001654"
     CDS             complement(21195..21785)
                     /locus_tag="IWJ77_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9453261.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6296 a         4462 c         4158 g         6997 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcaaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 agaaagcaca ggtgaaggtg gcgaaccaga agtgatcggt tcaacagaag aataatactg
     3541 aaacaatcag gtggtgttag aagcggcggg tcagcagctt ttaacacgcc ttttttattt
     3601 ctaatcgttt attttcatat ggggcttcca cttttgtaaa tggctttaca aacgaaaaat
     3661 cctaatttac caagcttttc ctcgcatact tttatcgtac ctttataaaa atttatactt
     3721 tgaaaaaaag tattcaatta ttgttgcatt tttcctgatt ctttattaga atgaaccctc
     3781 gttgaagtaa gtatttctag tttatcctag gattaattat aaaaataaaa cattcgttga
     3841 ttcgaaggag gttaaacaga tgtttgaaag ttttgatagc agccgcaacc gttacgcttc
     3901 agttggtgtc gtatcaagtt tgccagatga attgattgac agtatatggt ttattattga
     3961 cctagatctt aagggagtca ttccattaga aaatatatta acctttgatt tgatcgaaaa
     4021 tcgtggaaaa gtgacgatgc atttttctca agcaggaagc aatgtggaaa tggatattga
     4081 tttgccgttt gcctattcga accaatttcc tgctcaagta tacgcctatg atgacggtac
     4141 gagagaaacg atccttttac caagtgagat tagacaatat taaggtgata gataaacgtg
     4201 gatgtccctt attttacaga ccaggccaag ttgttgattc tttgactcaa ttcttggctt
     4261 ctttttatgg agtttcataa attatcattg atttctaatt ggtattctca ggataaacgc
     4321 tatatactac taactaaatt ttaaagatat aaggagttta aaagatgcgc gatttaacaa
     4381 aaggaagccc cgctaaactg attttgatgt tcactgttcc tttactgatc ggaaatgttt
     4441 ttcagcagtt ctacaatatg atcgatatga tcatcgtggg tcagacgcta ggtaaaaatg
     4501 cgctggcagc ggttggtgca actggaagcc tgacattttt gatcattggc tttgcccaag
     4561 gtttaacagc tggtttagcc attattaccg cacaacgtta cggtgcaaaa gattatcggg
     4621 gtctgaagaa aagttttgct gctagtgttg tcattagtct tgtcgtcact gtgattttaa
     4681 ctgtcctaag tctcctgttt attcgtccga tgcttcaatt gatgcaaact cctcctgaaa
     4741 tacttgatca agcacaaaca tttatttcaa ttattttatt agggatcttt gcatcagtca
     4801 gtttcaattt gctttctaat gtcattcgcg cacttggtga cagccgcaca ccactattct
     4861 ttctgatcat tgcggtcatc atcaacgtcg tacttgattt gatctttatt atctattttg
     4921 gcatgggtgt ggaaggtgca gctattgcta cagtaatcgc acaagtttct tccagcgtac
     4981 tgtgtctagt gtatatcaag aaaaaaatgc ctttgttaca gttacgcaaa aaagatttca
     5041 aatttgaaaa agaagagttt gctgtacacc taaatgctgc tttgccaatg gcgtttcaat
     5101 cttcgatcat cgctgttggt gctatcgtgt tacaagcagc actaaatagt ctagggacag
     5161 atgtcgtagc tgctcaagcc gctgctggtc gtatcgacca atttgcaaat cagccgatga
     5221 tgtcatttgg tattgcaatg gcaactttca ctgcacaaaa ctatggtgcg aaagaatatg
     5281 gccgaatctt gcaaggggta cgtcaaactt tactgatgag tgtcggattt agtctcttag
     5341 caggtgctat cgtcattttc ttcggtcatt tctttgttca attgtttgtt agcccatcag
     5401 aaacacgtgt atttgaatta gcacaaacct acttcaatat caatgggtct ctttactgga
     5461 tcttagcagt cctgttcatt ttacgctaca cacttcaagg gcttggacaa agcaagatcc
     5521 ctacgatcgc cggtatgatg gaattgctta tgcgttcttt tgcagctatc gttcttacag
     5581 gaatgtgggg atacccagga gcagcagcag cttctccttt agcttggatc ggttctgttg
     5641 ccgtgttgct ttactcttat atgcgttcga tgaaacaatt gaaacacatg ggtgaagagg
     5701 aacatttcac attagaacat gcagaagcaa gatagccttg attaaaagaa atcgttgcgg
     5761 ctaaaaatga aaacttttat tgaaaatatc ttcttcgttt accgaaaaca ggttttttag
     5821 ataacaaaaa ggctgtgaca aaaaatgtca cagccttttt tcttttattc tgtttttctt
     5881 tccgtggact ctactttgaa ataatcgata tgtgcatgct ggtattctaa taccgtgaaa
     5941 cggaatgatg caatctcaat gattgttcct gcttttattt ctttatatcg gctagaagca
     6001 aagcccgcaa gtgtagtaaa attcgtttct tcaaactctg gaatctctac atgaaaataa
     6061 cgctggaaat catctaatgt tagtttgccg gagatgatat aagagccatc tgactgttcc
     6121 gctatcaatg cttcgtccaa atctgtctca tctcggattt ccccgacgat ttcttctaaa
     6181 atatcttcaa tcgtcactaa gccgcttgtc cctccgtatt catcttttaa gatagcaata
     6241 tgcttaccct tttttcgcat gatcgtcaat aattttttta tagggatcgt ttcagttgtt
     6301 acgatcggct cttcaatcaa ctgttctact gtcagctggt catctttata agattgcttg
     6361 atcaaatctg gcaaggtgac ataacctaaa acattgtctt ttgattctaa gataaccgga
     6421 tagcgagtat gtccttgttc aatcgaagct tttatcgctt ctttgacggt ttctgaagct
     6481 tcgaatactt gcatggacgt acggtctact tgtatatctt ttgccaaggt atcatcaaat
     6541 tcaaacacgt tttccaaata ataatattct tctttggtca actctccttt ttcataagaa
     6601 ttcaatgcga ctaatgtcag ctcttcttga gacaatgtct cttctccttc agatacgaga
     6661 cgcacaccta atagcttccc tagccctgca gctgaatgat tcaataccca aataaaagga
     6721 aacattactt tataaaaata atgaagtggt ttaactacga acaagacgac cttctccgta
     6781 ttgacgatac tatagctctt aggaagcagt tcgccaataa ccacatcgat atacgtaact
     6841 aaaataaatc ccagcgccac agagattgta ctgctaatag ccgaactaaa aggtagttta
     6901 ccgatcactg gttctagtaa agccgcgaca gatccttcac cgacccaccc gatgatcaaa
     6961 ccggttagcg taattcctaa ttgactagct gataaatagt tatctaaatg gtgtgtaaca
     7021 tgaagagcta gcttggcgtt ctttacattt tccttttcta gttgttccaa tcttgatcgt
     7081 ctgattttca ctaaagcaaa ttcacttgct acaaaaaatg aagtaattgc aatcatcaaa
     7141 aataaaataa tgaaattgat aaccatattc tcccgccttt ctctttttct attgtaccct
     7201 ttttctacaa aaagattgaa aataatgcgt attcatattt aggaataaaa aaggagatgc
     7261 ggcatttttc agccacatct ccttatcact tcaaaatcat tgcgttactt gttgctaaac
     7321 agctttttca tgacctctga taaaaacggt attcaaaagc ttgcatctac ggcattaagc
     7381 ccaaacagat gaaaaacatg agaagctgtt tttatctgct cgttcttact gcttgttgca
     7441 gtacagcttt ttcatgacct ctgataaacg gtgttcaagt agctgacatt cgacattagt
     7501 tcaggtcaaa gcgctacttt cacaaccgct ctttcaacca tgttttccga gtatttccaa
     7561 tgataagcgg aacagtttca tgttttactt cgctgtactc tagcccaaac catgtttctg
     7621 gcgaggtagc gatttttttg attgccagtt ttgcctgcat gatttgacgt tgccattttc
     7681 ctagagttgt cacattggaa actacttcat caatcaggac aaattggaaa tcgccaactt
     7741 ctcgccctgg tgtcagagag tatctttgtg gctgttttgg caaacggcct tgtttcatca
     7801 aatcatggat gacttggcga acatatacgt tgatttcctg ttggaccctg aaacctaaga
     7861 acagattcac ttggacaata aaatcagtgt ccatcatatc tacttgatat tctttcgtat
     7921 atggctcatc cgtcacttcc acatgaacaa accaatagac tcgtgctctt ttaggccgtt
     7981 tatctaaaat cgaatagatg aactgtcggc caactttatc ttcagccata tcaggaacca
     8041 agaagaccac atttgtctga tagattggta aagaagtatc atcacgtaat tctctaagct
     8101 gcgggatata atctctcaaa gacacatcct cggttgacga ttcgcgaata atgttccctt
     8161 gttcccaaat aaacatcacg actaagatga tcaaaccaat cagaacggct acaaaaccgc
     8221 ctcggaagaa tttgacaaca cttgagataa agaaaacgac ttctatcgca ccgaagaaaa
     8281 aggtaactaa atatgcaagc caacgagaat gcccattttg gataagataa aagtaaagca
     8341 gtgctgttgt catcaacatc gtcacagtga tcgatagacc gtaagcagct tccatatgat
     8401 gagacgttcg gaatgtgaca accactagag aacaggcgat ccataaaatc agattgactg
     8461 ctgggatata catttgcccg atactgttcc caggatacat gatctttaac cgaggcagca
     8521 gtttcaactt gattgcttct gaaactagcg taaacgaacc tgtaatcaat gcttgcgaag
     8581 caatgactgc agcgactgtt gcaaaagcaa cgccgatcac gatccaactt tgaggcatca
     8641 tttggaagaa tggattcagg ttcttgattt gctgtgcttc agggttttga taaacattca
     8701 acaaccacgc agcctgccca aaataattga taaccaaaca aattttgacg taaggccagc
     8761 tcagacgaat atttcgtttg ccgacatgtc caagatcgga ataaagcgcc tctgcacctg
     8821 tcgtagccag gaaaatattt ccaagaatga acaagcctaa tttgttatct gcactaaaaa
     8881 gcagctggat agcataataa ggattcagtg cacggataac tgtccagtct tgactgaagt
     8941 tcattaatcc cattacgcct aaaaaagtaa accaagcgaa catgattggt ccgaatgctt
     9001 ttccgacaat atctgtacca aagcgttgga ccatgaataa aacaaaaata ataactaaag
     9061 tgataatgac gatcacgttc tggttattac cgaaccggtc aaaaaactgc ggaattcctc
     9121 gcaagccttc aatcgcagtc gttaccgtca cagcaggggt caatacccca tctgctagta
     9181 aggcagctcc tccgatcatt gcaggaatga tcaaatattt cccacctttt cggacaagtg
     9241 tgtagagtga gaaaattcca ccttcaccat ggttatctgc attcaatgca atcacgacat
     9301 atttgattgt tgtcagtaga gtcaatgtcc aaaaaatcaa tgagactgat ccgataacaa
     9361 aatcaggatt gattcctgac aatccgccat tatcttcaac aatcgatttc ataacgtaaa
     9421 gcggacttgt cccgatgtct ccgtagacga ctcccatcgc tacaagcagg ccagtcattg
     9481 atatcttttt ggtattgtgc gttgttgcca cgtttttccc tcatttcttg ttatatggat
     9541 atcctttagg taagcttgaa tgatagatga tattcttatc catgacaccc gctttcctaa
     9601 attgcttcta attatccaag taacacactg aagtgtcaac ttatttgaat aaaattattc
     9661 tttctttgga cgttctctga tacgtcttgc cacaaatgaa taaagagcaa ggcaaaaaat
     9721 tgcacccaat aatgctggta tcaaataaaa accgccgagt tccggtccca gtggtcctag
     9781 taaaaattcg ccgagccaac tacctaaaaa accgataatg atatttccag atacgcctcc
     9841 cgggacttcc tttcctaaaa ttgcgccagc tatcaaacct aatataccgc cgataaataa
     9901 cgaccataaa aaattcagca tctcctttcc ctcctttatc catatttctc acttttattt
     9961 tacgctttat tcagaaggac tccaaaaata tgcgccaaga aactccagaa aaaaagaagt
    10021 tgtaacaaaa gtgttcagct tatttccgta gaagctgctt ctggaacacc gtgaatcaga
    10081 ggttgtgaaa tcagcggttt gacctgagta ttagagaaac aaacggaaaa atagttcctc
    10141 atattttgcc gttttttgag ctaatatcga gggacagctg attgaacatc gtttattgga
    10201 gaggttgtaa aaaagctgtt ctgcatcaag taataagaac agccaaataa aaacagctcc
    10261 taatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat
    10321 tcgaaaaaaa aggactgtga cacggctttt gtcacagtcc ccttttttcg aataattatt
    10381 gtccccctgt caccttcacg atcacaagta cattattcct atgaattaaa ttatttttct
    10441 tcatttttcg ttgaaccatc ttcgttcaac attgcttttc ttgacagatt tacgcgtcct
    10501 tgtttatcga tttcagttac tttcacaagg atctcatcac ctagtttaac aacatcttcg
    10561 actttattga cacgatcgtt tgctaattgg gaaatatgga caagtccatc ttttcctttg
    10621 atcaagttga cgaatgcacc gaatttttcg atacggacaa ctttccctag ataaacttca
    10681 ccaactttga cttctttggt caaatcttcg atgatcttga tagctttttt gatcatttct
    10741 gcatcagctg aagcaatact tacttttcca tcttgatcga tatcgatttt cacaccagtt
    10801 tcatcgatga ttccgttgat cgtatcgccg cctttaccga taacatcttt gattttagca
    10861 ggttcgatct ggatcatttc gatttttggt gcatattggc ttaattcttc acgtggagca
    10921 gcaatcgttg aagtcaattc ttctaagatt tccatacgtg cttgttttgc ttgagttaat
    10981 gcttcagtta ggatttgttc tgtgattcct tgaatcttga tatccatctg taaagcagtg
    11041 ataccatctt ttgtaccggc aactttaaag tccatatcgc caagatgatc ttccagccct
    11101 tggatatcag ttaggatcgt atagttttca ccatcagaaa caagtcccat cgcaataccg
    11161 gcgactggcg ctttgattgg tacacctgca tccatcaatg ctaatgtgcc agcacagata
    11221 cttgcttggg aagaagaacc atttgattcc aatacttctg ctactaaacg gatcgtataa
    11281 ggaaattctt cttctgaagg gattacttgt gctaacgcac gttcacctaa tgcgccatgt
    11341 ccgatttccc gacgccctgg agagcctgca cgtcctgtag aaccaacaga aaattgaggg
    11401 aaattgtagt ggtggatgaa tcgtttgctg acttccaccc ctaaaccatc aatgatttgg
    11461 tgctctccta gaggtgcgag tgtacaagct gataaagctt gtgtttgtcc tcgtgtgaac
    11521 aaacctgatc catgtacacg tggcaataag ctgacctctg atgacaatgg acggatttca
    11581 tccaatttac gcccgtcagg acggatctta tcgatggtga tcaattcacg tacgacatct
    11641 ttttcaagat cttctgtgat ttgtttaact tctttcaaca agtgatcttc atcttcatga
    11701 cctgcaaatt tctcagcata agcttcgcgg atatcgatct tcacctgctc gatattatct
    11761 tcgcgagcaa gtttttcttc agtcattact gctgctttca ttgtagcgta agaagcatca
    11821 aagatttctt ttttcaatac ttcatcaact tgcagtaaag tcacttccat tttttctttc
    11881 cctactgctt gaacgatttc ttcttggaaa gcaactaact ctttgatagc gtcaaaacca
    11941 aacagcaatg caccaagcat gtcttcttct gaaacttctt tcgcgccgct ttcaaccata
    12001 ttgatggctt gtttcgttcc ggcaacagtc aattcgatat ctgtttgttc tgcttgttct
    12061 actgttggat tcaagacgta ctcgccattt acacgaccaa cttctacgcc tgcgattggg
    12121 ccgtcaaatg ggatatctga gattgatagt gctaaagaag aacctagcat tgctgccata
    12181 gctggcgagc agtctgtttc tacactcatc acgatgttcg ttacttggac ttcattacgg
    12241 aagccttctg caaacatcgg gcgaattgga cggtcgatca aacgggcagt caatgttgct
    12301 tccgtacttg gacggccttc tcgtttgatg aaacctccag ggattttccc tgctgcatac
    12361 attttttctt cataattgat cgttaatggg aaaaagtctg tatcttttgc ttctttagag
    12421 gccacagctg cacttaagac tactgtatct ccataacgga ctaaaacggc tccgtttgct
    12481 tgttttgcta gctgaccgac ttcgatttgt aagggacggc cgccccatgt cgttttgaat
    12541 acttgttttt ctgacataac ttctcctttt ccttctcgtg aaagcaagac gctactaaac
    12601 aaatgaaaac tgccgaaagc cgtcttttga gcagctttca tttggttagt aggagtccat
    12661 ctagcttgat cacggaattt tacttaattg atatacgagt aaaaaaagtg agattcatgt
    12721 gaatcccact ctttttgact aaaattaacg acgcaagcct aaacgtttga tcaattcgcg
    12781 gtaacgttga acgtcagttt tacgtaagta agctaacaag ttacggcggt gaccaacttt
    12841 tttcattagg ccgcggtatg aatgatggtc ttttttgtgc acgcgtgcat gttcattcaa
    12901 gtggttgatt tcttcagtta atacagcgat ttgtacttct ggagaaccag tatccccttc
    12961 atggcgagcg tattcgttga tgatttcatt cttgcgttct tttgaaattg ccatttcttt
    13021 cacctctttc gattattccc ctgcgactga gtaaaacgtt ggtgattcgc ttaaccaagt
    13081 ggcaggtcat gtgcctagag cacacagcta tactttacct gaaaccgggt tgaaaatcaa
    13141 gtagttaatc attaagaaaa ggtagttttc aaacaatctt actactactt ttccattttt
    13201 gtggtaaact ataatgcgaa aatagacttg aaacacagtc ttttaagaaa ggggtacata
    13261 ttccatgatt acaatggatg atatcattcg tgaaggaaat ccgaccttac gcgaagttgc
    13321 aaaagaagtt tctcttccat taagcgaaga agatatctct ttaggaaaag agatgcttga
    13381 gttcttaaaa aacagccaag atccaatcaa agctgaagaa ctacacttac gaggcggtgt
    13441 tggcttagct gctcctcaat tagatatttc taaaagaatc attgctgttc acgtaccaag
    13501 tcctgaccca gaggcagatg gaccttctct aagtactgtt atgtataatc ctaaaatttt
    13561 gagccattct gttcaagatg cttgtttagg ggaaggtgaa ggctgtttat ctgttgatcg
    13621 agaagtacca ggatatgttg ttcgccatgc caaaatcact gtgtcttatt atgatatgaa
    13681 tggtgaaaaa cacaagattc gtttgaagaa ttatgaatcg atcgtcgttc aacatgaaat
    13741 cgaccatatc aacggtgtga tgttctatga ccacatcaac gatcaaaatc catttgcact
    13801 aaaagaaggc gtattggtga tcgagtagag aattggcagg ttccttcgat actatgctac
    13861 aagaaagctt tataaaaaag gggagtgctc aaaagctgca cttccctttt ttatgtttct
    13921 ccactaatcg aaatttagat agacatctag aaatcgcaat tccaaaaaat tctgtgtatt
    13981 ggatacgcat cttttcttat tttttcttat ttcttccata tgggtgttta tctcagagat
    14041 gatagaatag tttctaggat aaaaagtagc agctacgatg ttatccttcg tagaggattc
    14101 ctgcttcagc tagtttttgt ttgatgtttt tcagcatatg cttttctggg tagcggatcg
    14161 tatgcagatg gatgccgcct gttaattcgg ataacaaagt agcattactt ttttgataac
    14221 gtctcataaa ggaatcgata tcttgtggcg ttttgatgtg caaagttcct gctaactcac
    14281 cgtaaagggg atgttccaca attacatcaa tgatttcacc accattttca acgattagtc
    14341 tcagttcttc ttctgtttga tcttttccat gctggacagc gattttactg atgtgcccag
    14401 tattggtttc ttctgtttcg aggatataac ctcttgctgt tgccacgata cttttacctg
    14461 cagctcgtag taaagcaata tcgcctacaa tgatctgacg gctgactcca aaagttttcg
    14521 caaacttgct agcactcact ggtttttctg tatttgccaa ggtttgcatg atttgttttc
    14581 gccgtgtctc tccttcgatc acacttattc ctccttcatt ttgttttggt attcgcgcga
    14641 gtgtttcatc aaatcaacat acgtgtctgt tattgtttcc ttatatccct tatttttcag
    14701 cagactaaca actttatcca atacttgctg ctctcttttt gcatcataaa ctgcttggtt
    14761 ttcttctttt ttcacttgtc ctattttagt taccgtatcc attctttttt ctaataaagc
    14821 aaccaactct cgatcgatca tatcgatctt atgacgtaat gtttcaagag actcttctgt
    14881 tagttcttcc tctactgtct tgatgatacg agcaggattt cctgcaatca ctacattgtc
    14941 gggaaaagat ttggtcacaa ccgaacctgc gccgactact acgttgtccc ctagagtgac
    15001 acccggcgtg acgataactc ctccgcctaa ccaaacatta ttaccgattt taatgggttt
    15061 tgcgtactcc agaccgctgt tacgtttcac tggatgtagt ggatgcgtcg ccgtgtaaag
    15121 ctggacattc ggagcgaaca tgcagttatc accgatttca atcgtgctga catctaaaaa
    15181 tgtacaattg aaatttgcat aaaaattctc cccaacaaat atattatatc cgtaatcaaa
    15241 attgataact ggctccatat agattttttt ccctgtacgg ccaaaagtct ctttcaacaa
    15301 ttgggatcgc agttccgctg attctgcttg gttgatgatt tggctttgac ttcttgcaaa
    15361 catacggtcg gcaaccagct caggatcatt ggcaaaataa agctcgcctg caatcatttt
    15421 ttctttttcg gttttgaatt cagtcataat ctgctcctaa ttcttgtatt tttttgaaaa
    15481 caaccgctcc gtcttcttga ataaacgtat aaggctccgc taaataaaca ggcgtttgat
    15541 tgattactaa gattttggct gacggctggc gaaactgtac cagatcacaa aacggatgaa
    15601 cttgaaaact cgttccaaca atgacgatca gctctgcttg gctgacggca taagccgcct
    15661 gctccatgac tccatcttga aacccttcac catataacac gatgtctggg cgtatttgtc
    15721 ctccgcatgc ttgatgtttg tcgctgtgaa gatattcttt ccagtctacc gtttgttgac
    15781 attttcgaca atgacattga tacagactcc catgaaaatt gacgagttgc gggcttttag
    15841 cctttgcgtg caaaccgtcg atgttttggg aaacaaccca aacatttttt tctttcgcta
    15901 gatgtgctat ttccaaatga atgatattgg gttttgcttc aggatgataa atctttttga
    15961 caaattgata gaatttatcc ggttcattca ccattgcttg atgactcagc aagtattccg
    16021 gctgctccat cccgtggtag attcctgaca aagatcgata gtctggcact ccagaaggtg
    16081 tggatacccc tgcaccggtc aaaaatgtaa tttgctgata agctaatatc tcttttgctg
    16141 cttcgtctac tgttaattgt ttcatgtttt gtcacctcac tttccattat agcccatact
    16201 cgaacaaatt tattgttgaa aagtcagaaa aatgggaata aaaaagtgtt atgggcattt
    16261 gagcaattta tcacaatgtt cactgacaaa agaatgaaaa cgtgttacaa tactgctgta
    16321 taaaagtgag gtgaatcgat atgttaccat tctttattta ttggggcgtt attctggctt
    16381 gtatcgcctg gttagcgtta agcgtttatt tctctgtttt ttatcttgta cgcaaagaaa
    16441 atggaaatct ttgggctttt gctttattca acgtgattgc cgcaattgtt ttagccatta
    16501 cattagccgt atatcgaaca tggggctggg gaatcaccca atactcaagt ttgatttatc
    16561 taatattagc catttatggc gtcgttgttc ttttgcaagc gatcctagga cgtgagccaa
    16621 agaaagcggc agcttaaatg atcgagatgg aactggacac aggaaatttt ctgttctagt
    16681 tcttttttta ttgtattaca tacatttttg tgtataatta tactatcgtt tgcaatggat
    16741 agatgattca cattttgatt gttttttcgg taaaagtaaa gatttctata tttatcagta
    16801 ttttttcaaa aaccagcagt tatagattga caaagcctct cgagtatcgt aaactttttt
    16861 tgtataaata actgaatacg agatgcctca atcaatgagg tagaggtcgc gagaatcatt
    16921 aaccctgtgg agtgaagcaa acatggagaa gcaggaatag ggaggatcgc cgaaatgtgc
    16981 acttttttgc ttggagtgta tgttgggacg caagttaaga gcttgcggac tgtcttagca
    17041 gtgatgctaa gttgcgctat gttttgtgag aggtttatca ccttatggaa ttttagcggc
    17101 ctttttatat ctataaatcg gtcgcttttt attttttcag ataaagcttt accgcatttc
    17161 atctagaccg ctcgtgtatc atataatcag aaagggagaa aaaaatgaaa aaaaatatgg
    17221 tttcagttgc cgtactattt gcttttgtct tctcactatt cagtgtggga tctctggcaa
    17281 atgcagatga aaaaacaccg agcgatgagt ttcgcgtagg tatggaggct gggtatgcac
    17341 ccttcaactg gtctcaacaa acagatgaga ataatgcctt gccaattcaa ggccaaaata
    17401 gttacgccgg cggttatgat gttcaaatcg ctaaaaaagt agcagatggt ttagggaaaa
    17461 aattagtcat cgtccaaacg aaatgggatg gtctggctcc tgcccttcaa tcaggcaaga
    17521 tcgacgcgat catcgccgga atgagcccta ctgctgaacg acgaaaagaa attgatttta
    17581 ccgatcctta ttacgaatca caattagtag tagtagttca aaaagatagc aaatatgcaa
    17641 aagcaaaaag tttagcagat ctttctggag caaagatcac tgctcagtta aatacattcc
    17701 actatactgt gatcgatcag ataccagatg tgcagaaaca acaagcaatg gataatttct
    17761 cagcaatgag aacagcttta gcttctggaa tgatcgatgg ttatgtcagc gaacgtccgg
    17821 aaggtgtaac ggcaactagt gtgaacaaag acttggaaat gcttgaattc agcggtgcag
    17881 atggcttcca gacaaacccg gaagatgttc agatttctgt tggtatgcgt aaaggcgacc
    17941 cggatatcca aaaagtaaac cagattcttt ctggcatctc taacgatgaa cgaaccaaaa
    18001 ttatggacca agcagtcaaa gatcagcctg ctgcaactga tagtgaagaa aaacctggca
    18061 tcatttctga tttcactacg atttggaaac aatacggcaa tatgttctta cgaggtgctg
    18121 gacttacctt gttcatcgca ttgatcggga cagttgtcgg tactacactt ggattactga
    18181 ttggtgtctt ccgttcggta cctgaatcag gaaatgcact gactcgcttc ttccaaaaaa
    18241 tcggaaatgc acttctttct atctatgtcg aagtattccg cggaacaccg atgatggtac
    18301 aagcaatggt catcttctac ggtctggcac tcgcttttgg catttcattg aaccgaacga
    18361 ttgccgcttt gtttattgtc tctctcaata ctggagctta catgacagag atcgttcgtg
    18421 gggggatttt tgcagtagat gaagggcagt tcgaagctgc gcaagccatt ggtatgaccc
    18481 atggccaaac gatgagaaaa gttgttattc cacaagtttt acggaatatc ttgccagcaa
    18541 caggaaatga gtttgttatc aatatcaaag atacagcagt tttaagtgta atcggcgtag
    18601 ctgatttgtt cttccaagga aactctgctt ccggtgcgaa tttccaattc ttccaaacat
    18661 ttacgatcgt cgggatcatc tacttagtca tgacctttac gatcaccaga atcctgcggg
    18721 cggtagaaaa gaaaatcgat ggtccttcag cttataccaa gcttgaagaa atcgacaatt
    18781 caaatttgca ggaataagga ggaagaatca tgaatgaaaa aattattgaa atcaaacatc
    18841 taagaaaaag cttcggtgaa aatgaagtat tgaaagatat cagcgtaaca gtcaataaag
    18901 gtgaagttgt gacgatcatc ggttcttccg gttctggtaa atctactttc ttgcgctgta
    18961 tcaatttact ggaaaaacca acaggcggcg agatcattta taatggagaa aatgtattag
    19021 ctcctaaata taatttacct aaatatcgaa cgaatctagg tatggttttc caatccttca
    19081 acctgttcaa caatatgaat gtcttggaaa actgtatgtc tggtcaaaca actgtcctga
    19141 aacgagataa agaaactgcc aaaaaagtag caatcgaaaa tctggaaaaa gttggtatgg
    19201 atcgttatat cgaagccaaa ccagcacaat tatctggtgg acagaaacag cgtgtcgcaa
    19261 ttgctcgggc attatcaatg aatccagatg tattgctatt cgacgaacct acttcggcgt
    19321 tagaccctga aatggttggc gaagtgctta atacgatcaa agacttagca catactggct
    19381 tgacaatgct tatcgtgacg catgagatgg aatttgcaaa agatgtatct gaccgtgtca
    19441 tttttatgga taaaggtgtg attgcagaag aaggtacacc agaagatatc tttgtccatc
    19501 cgaaagaaga acgtacaaaa gaattccttt cacgtatttt aaatgcttag gatcaatagc
    19561 tgattgggac aaagagctgc ggtgtttacc gcagttcttt tttatgaatt actcataaaa
    19621 ggtaaatttc tgaaaatttt ggaatcttag ttgtgttttt cagaaaactc tcttaatatg
    19681 gaagtaatta aactttttta gttaggatga tttaaaaatg aatgaagagc aagaactgca
    19741 gcgtggactg aaaaatagac atgtccaact gatttctatt ggcggagcga ttggaacggg
    19801 gttgttttta ggttccggaa agtctatcca gctagctggc ccttcgatat tgctcgccta
    19861 tcttattact ggatgcatct gttttttcat tatgcgtgcg ttaggcgaac ttctgttatc
    19921 aaacaccaat aaccattctt ttctggattt tgttgcagaa tatcttggca aaaaagcggc
    19981 tttcatcact ggctggacgt attggttctg ctgggtatcg attgcaatgg ccgatttaac
    20041 agccatcgga atgtatgtac gctactgggc gcctggtgtt cctcagtggc ttcctgaact
    20101 gattgcatta ggtcttcttc tttgcttgaa tatggtggct gtctcattgt ttggtgagtt
    20161 agagttttgg tttgcattga tcaaagttgt agcgatcatt gcctttatcc tcgtaggagc
    20221 ttatatgatc ctcacccact atacgacaga tattggcaca gccagtatca cgaatctatg
    20281 gtctcatggc ggattctttc caactggtgc aaaaggattt atcttagctt tccaaatggt
    20341 aacctttgct tttgctggga tagaattagt tggattagtc gctggagaaa cagaaaatcc
    20401 tgaaaaagtg ttgccggaag ctatcaataa tattcctatc aggatcatct tattttactt
    20461 aggatctctt tttgtcatta tggcaatcta tccatggaac agcttgaatc cagataacag
    20521 tccatttgta gaagtatttt cggaaattgg gatcacgatt gctgcttcct tgatcaattt
    20581 agtcgtatta agtgcagcag cttctgcctg caacagtgca atatatagta caggacgtat
    20641 gcttcgttct ttagcgcaag aaggcagtgc ccctaaaaaa ttcaaaaagt taacaacaca
    20701 tcatgtgcca gggaatgctt tgacattttc tactatcgtc attttcattt ctgttatctt
    20761 gaattacgtg atgcctagtg aagtattcac actagtaagc agtattgcca ctacttgttt
    20821 cttgtttatc tggagtatgc ttgtctacac gcacatgaaa tatcgtaaaa gtatcctcgg
    20881 caaaaaagct cattcattta aaatgccgtt gtacccattt agtaattatc tggtatttct
    20941 ttacatggcc tttgtgtgtg tcgtcttgtt cctaggaaaa gatacgagaa tcgctttatt
    21001 actaacacct gtgtggttct tgcttctatt actaatctat catatgaaat atgaacgtaa
    21061 gaagtaagaa agtagttgtg acagaagtgg tcagcttcga gaaataagaa ggaatttacg
    21121 aaaattgctt cttaaatttt tgtgaaattt cggcttattt cttggagata aaagcttctg
    21181 tcccagcctc ttttctattt ccccggaaac aaccgctgat aataggcatc tgctttttta
    21241 taggaaaaaa tccaaatacc agatacaaaa aaacgtccga ataacccgat caacggtaca
    21301 aaaaagacac cggcaatcaa gcataagtta atcatcatgg agataagact tcgacggtcc
    21361 tttgacaaaa ctctcacgac atgggaatct ggtttatttg ctttcatgat ttgcctcatc
    21421 aagataccaa aagaaaaact ccacatcgtg tatactaaga cataagccat ctcaggccaa
    21481 aaagcttcag ggttacgccc gacccaacta gtaacagaag gacataaagt cagccaaaag
    21541 agcaaaaaga tattcgacca gaccactcgg taattaattc gttctgccat ctgaaatata
    21601 cgatgatggt tcacccagat caccgcaaca aatataaagc tgatggtata agctaataat
    21661 gtatttcgga tgacccataa agaatgaaag ctagatgtat tagggatctt gatctctaat
    21721 acaataactc tcaaaacaat ggctaccact ccatcagtaa atgcttctaa tctcgacttt
    21781 gtcacgatga ttcctcctca gatgattcag cgcttttatt ttatcactta cgttattaaa
    21841 taaccatcgt tttatatagg gaattttctg aaaattgttg catatttttc gaaattttgt
    21901 tacttttaaa aaa
//
LOCUS       DACDRK010000040        20556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDRK010000040 DACDRK010000000
VERSION     DACDRK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.040"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            297..1406
                     /locus_tag="IWJ77_001655"
     CDS             297..1406
                     /locus_tag="IWJ77_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9453262.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1489..2385
                     /locus_tag="IWJ77_001656"
                     /pseudo
     CDS             1489..2385
                     /locus_tag="IWJ77_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2401..3225
                     /locus_tag="IWJ77_001657"
     CDS             2401..3225
                     /locus_tag="IWJ77_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9453263.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3257..4567
                     /locus_tag="IWJ77_001658"
     CDS             3257..4567
                     /locus_tag="IWJ77_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9453264.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4715..5974
                     /locus_tag="IWJ77_001659"
     CDS             4715..5974
                     /locus_tag="IWJ77_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9453265.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5984..6856
                     /locus_tag="IWJ77_001660"
     CDS             5984..6856
                     /locus_tag="IWJ77_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9453266.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6868..7698
                     /locus_tag="IWJ77_001661"
     CDS             6868..7698
                     /locus_tag="IWJ77_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9453267.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7738..9921
                     /locus_tag="IWJ77_001662"
     CDS             7738..9921
                     /locus_tag="IWJ77_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9453268.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9982..11202
                     /locus_tag="IWJ77_001663"
     CDS             9982..11202
                     /locus_tag="IWJ77_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9453269.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11199..12659
                     /gene="gtfA"
                     /locus_tag="IWJ77_001664"
     CDS             11199..12659
                     /gene="gtfA"
                     /locus_tag="IWJ77_001664"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9453270.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12736..13842
                     /locus_tag="IWJ77_001665"
                     /pseudo
     CDS             12736..13842
                     /locus_tag="IWJ77_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            14164..14373
                     /locus_tag="IWJ77_001666"
     CDS             14164..14373
                     /locus_tag="IWJ77_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9453271.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            14366..14515
                     /locus_tag="IWJ77_001667"
     CDS             14366..14515
                     /locus_tag="IWJ77_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9453272.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            14550..15334
                     /locus_tag="IWJ77_001668"
                     /pseudo
     CDS             14550..15334
                     /locus_tag="IWJ77_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(15618..16703)
                     /gene="xerS"
                     /locus_tag="IWJ77_001669"
     CDS             complement(15618..16703)
                     /gene="xerS"
                     /locus_tag="IWJ77_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9453273.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            17037..17270
                     /locus_tag="IWJ77_001670"
     CDS             17037..17270
                     /locus_tag="IWJ77_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9453274.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            17267..17674
                     /locus_tag="IWJ77_001671"
     CDS             17267..17674
                     /locus_tag="IWJ77_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9453275.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(18811..19329)
                     /locus_tag="IWJ77_001672"
     CDS             complement(18811..19329)
                     /locus_tag="IWJ77_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9453276.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            20447..>20556
                     /locus_tag="IWJ77_001673"
     CDS             20447..>20556
                     /locus_tag="IWJ77_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9453277.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         6979 a         3170 c         3825 g         6582 t
ORIGIN      
        1 taaaaaagta aacatccaaa aaagggtgtt tacttttttg tttactaata tttttagtaa
       61 aaaatctcga caaaacatta agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat
      121 tcaaaacagg aggaattaag atgaaacatt tcaaaaaaat agctttaaca gggatagctg
      181 ttttaactgg gacattactc gcagcatgtg gtgggggaga tagtaattct aaagacgggc
      241 aagtaacttt aagctatgcg agttgagata ctgaccaagc tccaggacta agaaaaatgt
      301 tagatgaatt tgaaaaacaa aatcctgata tcaaagtaga aatggagaca acgccttggg
      361 atcaatattg ggttaaatta gaagctgcaa caacgggagg taatatgcca gatgtagtaa
      421 ccatgcactc tagcgaatcc tataaatata tgtcacagaa tgctttgatg ggacttgatg
      481 atgttatcaa agaaaatgat attgacatgg gaaactttac tgaaggaatt gctgatttct
      541 atacgcttga agacgaacta tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt
      601 acaacaaaga attatttgat aatgcaggtg tcgattatcc agatgaaaca tggacatggg
      661 aaacactcag acaagctgca attgatttaa cagatgaatc aaaaggcatc tatggttttg
      721 catcacatat tggaacagag actggctact ggccatttat ttatcaaaat gggggagaag
      781 tatttgccga tgacaataca aaatcaggtt tgaatacccc agaagcacaa gaagctttag
      841 ctttttatcg agatattgca cttgttgatc atgcttcacc aacaattaca gaattacaag
      901 atcctgataa gttaagtcgt tttcaatcag gtagattagc aatgatgatt gaaggaaact
      961 ggtttgcacc gggatttttg aaaaatgact atctagttga aaatgttgct tctgccgtgt
     1021 tacctaaggg aaaagttaat gcaacaatta caaatggact tggttgggct gccagtgcga
     1081 gtacaaaata cccagaagaa gtgaaaaaac taatggcatt tttagctagt gaggacgcca
     1141 ataaaatcca agcggaaacg ggggcatcaa tcccagcact aaaaggcttt gaagacgagt
     1201 gggctgcttc ttcagataca attgatttga gtgcttttgg aaaaatgttg gagtatggag
     1261 tccctcgtcc atacaataaa gatggattac gagctgaaca agcagaagtt caaattatta
     1321 accgtattct gtctggtgaa gtaactgttg aagatggaac cgctcaaatt gaaaaagaag
     1381 tcaatgcgat attgagcgga aattaaatta gttaattata caatatacag gcgaaaggat
     1441 tctatttcgt ctgtatatta atggatgaat accaaaaagg gggatattat gaaaaatcga
     1501 atttttggaa aatatcgtaa agatcaagtg atatggggct atatttttat ttttccaacg
     1561 gcattaggat taattttatt aaatatatgg ccagcaattc aaacagcaat tttaagtttt
     1621 caaaaaacgg tagggttcgg tgatactgaa tgggtcggat taagaaatta ccaaaaaatg
     1681 tttgcagacg gtgaagtttt tcagtcttta ttaaacacat taatttatgc agtggtttcg
     1741 atacctagta ttgttgtatt atccttgttg gtggcagttt taatgaatag aaaagttaaa
     1801 ggattgtcga tttatcggac aatttatttt ctaccagtcg ttgctgcacc atccgcagtc
     1861 gcaatgattt ggcgctggat gttcaataat gattatggac tgattaataa catgttggct
     1921 aaaataggtc tcgatggacc agcatggtta acggatccat cgattgcgat ttattctatt
     1981 atcattgtag gagtttggag tgctattggt tataacatgg tgttattact agccggctta
     2041 taagaaattc ctaaagatta ttatgaagca gcggaaattg atggtgctag tccaatccag
     2101 cagtttttca acattacgtt accattagtt acaccaacat tattttttgt agttgtaaca
     2161 acagttatca atgctttcca agtatttgat gttattttta tgatgattac tcctaatagt
     2221 accgccatgt ttaaatcaca atcattagcc tatttattct ataaacattc atttatttta
     2281 aatgacaagg gatatggttc ggcaatcgtt atgttccttt tagtgattat attaattatt
     2341 actgcaattc aaatgaagtt gcagaaaaag tgggtaaact attaaggagg ctatcaaatt
     2401 atgaaaaata aatttttttc aaatgcgcca atccatatca tgcttatttt aggtggtatt
     2461 gcaatggcat taccttttat ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct
     2521 acccaaattc cacctacaat tttccctgag aaatttcagc taagtaacta tcaacaagta
     2581 cttgatttat taccattcat ggctttttat atcaatacat ttttaatggt tgttggacga
     2641 gtgattggct cggttttatt ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt
     2701 ccagggaaaa atatcctttt cagcttagta cttattcaaa tgatgattcc cggttccatt
     2761 tttattactc cacagtattt attagcacaa aaattgggta ttttaaatac aattccagca
     2821 ttaatattac caggggtagt tagtgcgttt ggtacgtttt tattaagaca atttttctta
     2881 ggtttacctg atgaattgga ggaagcggcc tatttagaag gtgccaatat ttggacgatc
     2941 tttttcaaaa ttatgttgcc gcttgctaaa tcaggattgg ttgcacttgg aatattcaca
     3001 gcgttgtttg catttaaaga tttaatgtgg ccattgattg tcaatacttc aattgataag
     3061 atgcctttag ctccgggatt agcaaactta caaggacaat atgccacgaa cttcccacaa
     3121 ttaatggcag gttcgttatt agcaatttgg ccaatagttg tgatattcat cgttttccaa
     3181 agaaaattca ttgaaggaat tgctacaaca ggtagtaaat tataaaacta ttaattagtg
     3241 attggagagt aagtacatga caaaaattac atttattggt gcaggaagta ctatttttgc
     3301 aaaaaacgta ttaggcgatg cgatgttaac tccaagtcta caagatgcac atattgcatt
     3361 gtacgatatt gatgaaaaac gactaaaaga atcaaattta atgcttaaaa atattaataa
     3421 aaattcgaat gaaaatcgtg ctgatattgt ttcttattat gatcgcaaag aagcgttaag
     3481 agaggctgat tttgttatca atgctattca agtgggtggt tataaacctt caacagtgat
     3541 tgattttgat attcctaaaa aatatgggtt gcgacaaact attggtgaca ccatggggat
     3601 tggtgggatt tttagaaccc tacggacact accagtaatg ttagactttg ctaaagaaat
     3661 ggaagaagtt tgtccaaatg cttggttatt gaactataca aatccgatgg cgatgttaac
     3721 catgggtgtt ttaaaagcga cgagtattaa gactgttggt ttatgtcata gcgttcaagt
     3781 atgcgtacca gaattgtttg agcatttagg aatagccgat caatatgact taaatgactt
     3841 tcaatggaaa attgcaggca ttaatcatat ggcattcttg ttggaaatta caaaaaatgg
     3901 ggaagatttc tatccaacaa ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga
     3961 ttctgtacgt tttgaattgt tgaaacgttt tggttactat attacagaat caagtgaaca
     4021 caatgcagaa taccatccat actttattaa atcaaaatac ccagaattga ttgacgaatt
     4081 agggattccg ttggatgaat acttacgtcg ttgtgaacaa caaattaatg attgggaaag
     4141 catgcgagag gacattgtaa ataatgggga gttaacacat acgagaagcc gtgaatatgc
     4201 ttcatatatc atggattcta ttacgacagg aactccgtca gtgattgcag gaaatgtctt
     4261 aaataaaggc ttaatcacaa acttaccgac agatgtttgt gtggaagtac cttgtttagt
     4321 cgataaaaat ggcgtgcaac caacctatgt cggtgaattg ccaacacaat tagctgcttt
     4381 aaatcggacg aatatcaatg ttcaagaact gacagtagaa gccgctttaa cactagataa
     4441 atcaaaaatt tatcaagcag cgtatttaga tccacatcta tcatctgaat tgtctttaac
     4501 agatatcaaa aatttagtgg atgatttaat cgaagcacat ggggactatt tacctgaata
     4561 taattaaaaa gaagctgaaa gagtaaggaa ttttcaattc cttactcttt tcttagttaa
     4621 atttagtaaa aatattgaca aaacacttag taaacagtta cattagtgtt aacaaaaaga
     4681 aaacgcattc aaattgtata aggaggagca taaaatgaag tggtctaaaa aaattatttt
     4741 agcttgtata gcactaggag caacgatctc tttgactgct tgtggaaaaa atcaggaaga
     4801 tgcaagcggt aaggtagaaa tagagttttt taatcaaaaa aaagaaatga ctcaaacgat
     4861 tcaggagatt gctaaagatt ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga
     4921 tgtaccgaat gctggtgaag taattaaaac acgtatgctt gcaggcgatg taccagatgt
     4981 cattaatctt tatcctcaaa gtatagaatt gaaagaatgg gcgaaagctg gttatttaga
     5041 ggacttaacc gaagaaccgt atttagagaa tatcaaaaat ggatatgcac aacgttttgc
     5101 tattgaggat cgtgtatata gcataccatt aaccgctaat gtctatggtt tttactataa
     5161 caaaactgct tttgaggaaa tggggattca agcaccagaa acttgggcag aatttgaaaa
     5221 aatagtagct gagattaaag atcaaaacaa agtaccgttt gcgattgcag gagcagaagg
     5281 ctggacttta aatggttatc accaactagc tatagcgact gctgctggtg gtgaagagga
     5341 agcgaacaat gtttggcgtt tctcagaagt taatggaatt aatgctgaaa gcaaagaaat
     5401 gcaaaaagat tttaatcgat tagatttgtt aagagaacca aaagctttgc aaaacaactg
     5461 gcaaggtgcc ggttataacg atacagtagt tacttttaca aaaggtgatg cgttgattat
     5521 gccgaatggt tcatgggcaa tgccgatgat tcattcacaa aatccagatt ttgaagtagg
     5581 aacttttcct tttccagcag atgaagcagg tcaaagtcta acgattggcg caggagacct
     5641 agccttatca gtttctgcaa cttctcaaca taaagaagaa gccaaacgtt ttgtagaata
     5701 tatgacaaca cctgaagcga tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt
     5761 tgagggcgtt atcgagaata ctcaggattc accattaagt ggtttaactg aacttgcttt
     5821 tactgatcgt catttagttt ggttagccaa agattggaat agtgaaaatg atttttatac
     5881 cttaacaaca aattatttac acaatggcaa tcaaaaagcc atgattaacg cattaaatgc
     5941 tttctttaat ccaatgaaag ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat
     6001 tttaaatcgt tactgggcaa tcatttttgt agcaattcca attattttgc agatgatttt
     6061 tttctacttt ccaatggttc aaggtgcttt ttacagttta actaattgga ctggtctgac
     6121 ttataatttt gattttgtgg gtttgaacaa ttataagatt cttttttctg atccgaagtt
     6181 tatcaaatcg attggcttca ctttaattgt gacgctttgc atgattgctg gacaaattgg
     6241 ttttggtatt ttgattgcgc gggcgttgaa cgcaaatatt aaagggaaaa cattctttag
     6301 agcatggttc ttttttccag cggtcttatc gggcgtggtt gtttctttag tatttaaaca
     6361 aatttttaac tatggtttac caaaaattgg tagtatgtta ggcattgaat ttttacaaac
     6421 cagtctttta ggaacaacat ggggagcgat ttttgcagta atttttgttt tactttggca
     6481 aggaattgcc atgccagtca tcattttctt agcgggtttt caaagtattc cacaagaaat
     6541 tattgaagct gctagaatgg atggggcgaa tgataagcaa attttttgga atattgaaat
     6601 cacctactta ttaccaagta tttcaatggt atttattatg gcattaaaag gtggcttaac
     6661 agcatttgat caaatttttg ctttaactgg tggtggacca agcgatgcca caacatcttt
     6721 ggggttactt gtttataatt atgcttttaa aaacaatcag tttggttatg cgaatgctat
     6781 tgctttagtc ttatttatct taattgggat tgtttcactg gcacaattaa aattatcgaa
     6841 aaaatttgaa atctaggagg tttcccaatg aaaacatcaa aagaaaaaaa tatttctaaa
     6901 tacttgcttt tattattagg gggcttcatg attttagttc ccttagtttc aacgctattc
     6961 ggttcattta aaacaacacc cgatattatg aataatttct ttcgttttcc taatccagcg
     7021 actttggaaa atttccgaag attgttagcg gatggaattg gtcattactt ttggaattcg
     7081 gcaatcatca caatcattgc agtagttgct gtgacagcaa ttattcctat ggctgcttat
     7141 tcactagcaa gaaaaatgac tcgtgggaaa gcatacggat tgatgtattt atttttaatt
     7201 ttaggaattt ttgttccttt ccaagtaatt atgattccga ttaccgttat gatgagtaaa
     7261 attggtttag caaatatgtg gggattaatc ttactctatc tcacttatgc cgtaccacag
     7321 acactatttt tatatgttgg ttttattaaa ctaaatgtgc ctatcagtct ggatgaggct
     7381 gctgaaattg atggggctag tcactttaca acttatttca aaattgtttt cccaatgtta
     7441 aaaccaatgc atgcgacgac tattattctt aatgctctat ggttttggaa tgatttcatg
     7501 ctaccattat taattttaaa tcgtgattct aatatgtgga cactaccatt gtttcaatat
     7561 aattatacgg gacaatattt taacgactat ggtccaagtt ttgcttccta tacagtagga
     7621 attgtcacga ttacactagt ttatcttgct ttccaaagaa gtattatagc tggaatgagc
     7681 aatggagcag tgaaataacc aataaattat tctcattata aaggagttca taaacttatg
     7741 attatatttg acaatcacac acaaacattt catttacaaa ataaaaaaat tagctatcta
     7801 ttaggaatcg aagaaaataa ttatttaacg catctttatt ttggaaaaaa gattacgcac
     7861 tattctggtg ggtatcgcta cccccgaaca gatcgttcct tctcacccaa caatgcaaat
     7921 gcgcaagatc gtttattatc tttagacaca ttaccattag aatttccagg atatggacat
     7981 ggtgatttta gagaaccggc gcataatatt aaattaggaa acggtagtcg tgtaaatgat
     8041 tttcgttatg atagctacga aattcaaaaa ggaaagccca gcttagcggg attgccagcc
     8101 ttatatacgg aagatgatga tgaagctgaa acgctgatta ttcaattgaa agacgcgatt
     8161 agtggattga ctttacagtt gttctacaca atttatcaaa atcacagtgt aattattcgt
     8221 tctacgcaat tagtaaataa tagtgatgaa acagtggaga ttaaccgctt agctagtcaa
     8281 tcaattgatt ttccaaatcg tccactcgag ttaattcatt taaatggtac ctgggcaaga
     8341 gaacgtcaaa tgactagaga aaaaattcat acagggataa aagtattaga tagtaaacgc
     8401 ggatcaagca gccatcaaca aaatccgttt gttgctttag tagatgccaa aacagatgaa
     8461 tttcaaggag aggcatatgg atttaattta gtttatagcg gtaatcacga aacggtgatt
     8521 caaaaagatc catttgacca aacacgggtc attatgggaa ttaattcatt taattttggt
     8581 tggcaactaa aaactggcga ctcatttcaa acgccagaag tcgtgatggt ttatagtaat
     8641 gaaggtctga atacgttatc acagacgtat catgaattgt ataatcaccg tttaattcgt
     8701 ggaaactatc gtttaaaaga acgtcccacg ttaatcaaca actgggaagc aacgtatttt
     8761 gattttaacg aagaaaaaat catgaatatt gtcgatgatg cgaagaactt gggcattgaa
     8821 atgtttgttt tagatgatgg ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt
     8881 gattgggttg aaacagaagg caaacttgct tgtggtttgg aaaatttagc caagcaagtg
     8941 catgataacg ggatgaaatt tgggatttgg tttgaaccgg aaatgatttc tgaagatagt
     9001 gaactatttc gagcacatcc agattgggca ttgggtattc caggtcgtga ccgctcaatt
     9061 agtcgaaatc aatatgttct tgatttttca agacaagatg ttcgtgataa tatctatcaa
     9121 caaatgacgg cgatttttga tcgtgtaccg attgattatg tgaagtggga tatgaatcga
     9181 aatatgacag aagtctattc tgtcttgttg gactcaacga tgcaaggtga agtggctcat
     9241 cgttatatgt tagggttata tgaattttta gaaaagttaa caacgaatta tccaactatt
     9301 ttatttgaaa gttgttcggg tggaggcggt cgttttgatg caggtttctt gtattacatg
     9361 ccacaaactt ggacgagcga caataccgat gccgtggctc gtctgaaaat tcaatatgga
     9421 accagcttag tgtatccaat ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat
     9481 caaacaggaa gagaaacgag tttaactatt cgtggcaatg ctgcgatggc tggtgttttt
     9541 ggttatgagc tagatctaac gcaattaact tctgaagaaa aagcagaaat taaagaacaa
     9601 gttgcctttt ataaaaagca tcgccaactt ttgcaatatg gaacttttta tcgcttggaa
     9661 agtccgtttg aaggcaatga tactgcatgg atgtttgtct caaaagacca aacagaagcc
     9721 atggtctttt atttccgagt attagcagaa gctgcgcatc cattagtcac attgaaatta
     9781 gcaggattag cagaagaagc aacttatcaa atggaagatg taacatttgc tggcgatgaa
     9841 ttgatgaatc tcggttttta tattgactca gaactacatg gtgattatgc gacacaacgc
     9901 ttttatttga aacagatata gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga
     9961 ctcattccaa acgaagaaaa tatgttttat ttacacgcat ttactaaaaa gcagcctgat
    10021 ttgaattggg aaaatgaaga agtacgtgaa gaaatctatg caatgattaa ctattggtta
    10081 gataaaggac taggcggatt tagaatcgat gcgatattaa acatcaaaaa aaaactagag
    10141 tatggcatct ttgaatctga tggagaagat gggttagcct tcattgggcc ctggattctt
    10201 aatcagtcgg gcattgaagt gtggttaaaa gaaatgagag agcggacatt taaaccacat
    10261 aatagtatga cggtagcaga agccgatgtt cctaatgaac gtttagatga atatattggt
    10321 gaagatggct attattcgat ggtttttgat tttagttata cggatattga tgtacctgaa
    10381 actggtgaat ggtttaaaga tagccaatgg acatggacgg atatgcgtac caatattttt
    10441 acgaatcaat tagttacgca agataaaggt tggggcgcct tatatttaga aaaccacgat
    10501 caaccacgtt caattaataa atatattcca gaagaatgga tcaatgatta tagtaaaaaa
    10561 atgttggcga cgctttttat gttactaaga ggtacccctt ttatttatca aggacaagaa
    10621 ctaggaatga ccaacataga aatggcatca ttagaagatt ttgatgatgt agctacgcat
    10681 tctcagtata aacgggcatt agaatatgga ttggcaccag agcaagcatt gaaagctgtc
    10741 aataaacgaa gtcgggataa ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc
    10801 agattctcaa cttctgataa tgtatggtta aaagtaaatc ctaattatcc agaactcaat
    10861 gcagaagctc agttaaccaa tcaacagtcg gtattcaatt attatcatca attaattaaa
    10921 ttaagaaaaa atagtgtata caaagacgtg ctaatctacg gacaatttct accagtgaaa
    10981 gataaaaatg aacatatttt gctttatgaa cgccaattag aagataaaat cgtattagtt
    11041 atcttaaacg ttacagcaac agaacaagaa tattatgtag atgaacagta ccaacaagta
    11101 ttaatcaata attatgaaga tgttttatta gatcaaagtc gaaaattgcg tttacgaccg
    11161 tttgaaagta ttgtattagc taattatgga ggacaattat gaaaattaaa aacgaagcaa
    11221 tgctcatcac ctatgcagat agtttaggaa aaaacatggg cgaattaaaa caagtcttag
    11281 acaaacattt acaaggtgtt attggtggtg tacatctgtt gccttttttt ccatcgacag
    11341 gtgatcgagg ctttgcgccg agtgattata cgacagttga tccagctctt ggaacatggg
    11401 aagatgtgga agcattaggt gaagattatt atttgatgtt tgattttatg attaatcaca
    11461 tttctcgtga atcaaaattt ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca
    11521 aagaaatgtt tatccgtatt catgaatttt tcccagaaaa tcgaccaaca caagaagata
    11581 ttgatttaat ctacaagcgt aaagacaaag cgccgtttca aacggtagaa tttgcggatg
    11641 gtacgacaga agaggtttgg aatacctttg gggaagaaca aattgattta gatgtgacca
    11701 aagaagttgt gaaagaattt attcgagaaa ccatcaaaga tatggcgaac catggctgtt
    11761 ctttaattcg attagatgcg tttgcttatg cgattaaaaa attagatacc aatgatttct
    11821 tcgtggaacc agatatttgg gaacttttag acgaagtgcg tcaagaagca gcgaaatatg
    11881 acgtcgaatt attaccagaa atccatgagc attattccat tcaaatgaaa attgccaatc
    11941 atgattatta tatctatgac ttcgccttgc caatggtcac tctgtattca ctttatagtg
    12001 gaaaatcaga acgtttagcc aattggttga aaatgagtcc gatgaagcag tttacaacat
    12061 tggatacgca tgatggaatt ggtgttgtgg acgctcgtga tttattaaca gatgaagaat
    12121 tggattacac ctctgaggaa ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa
    12181 gtgccaatta caataattta gatatttatc aaattaacag cacctattat agtgcattag
    12241 gcgataatga ccgtagctat ctgttagcac gtgcgattca agtatttgcg ccagggattc
    12301 cacaaattta ttacgttggg ttattagcag gaaaaaatga tattgaactg ttggaagcaa
    12361 cgaaagaagg gcgtaatatt aatcgtcatt actatgattt agccgaaatt gaagaagaag
    12421 tgcaacggcc agtgattcaa caattatttg cacttttaaa atttagaaat gaatctgcgg
    12481 cgtttgattt ggatggaacg attgaagtag caacgccatc agaatcagaa atagtgatta
    12541 cacgtaaaaa taaaaacggt agtgatagtg caacattaac ggctaacttg gatacgaaag
    12601 aatttatcat tgagtctaaa gatataaaaa ttttcactag tgttgcatta aaattttaac
    12661 ctattccata gaacaaaaaa tcctttatac tagcacttgg atgacttatc caagaccaat
    12721 aaaaaggact catacatgga taagtataaa acaaatacag catttaataa atggttttct
    12781 gccataaaat taggaaaact ttctgtcaac tcccaaaaat gtatttttga ttacaattcg
    12841 tatcgtaaaa aactgtcttt tgaagctgtg ctcaagctct ttctctacgc agttaatgaa
    12901 gaaaaagaaa gtttgcgtga tttgagtaca gctcttatcg accgatcgct ccaactagaa
    12961 accgatgtgt catcgattag tcatacccaa ttgtctcgag catttaatgc acttgaacct
    13021 aaagtgttag aagaaatttt tcaacaactg ttagaaaaag ttcaacatca aatcaaacca
    13081 accaagcgaa atcatctgta tctgattgat tccagtacgt tttcgcttag tttaaaacgt
    13141 cataagtggg cgactttccg tcaaacaaaa tcaggtgtca agttacactt aaactattgt
    13201 tatatggacg atactacgat gtatccaact gattttacct taacaaatgc ccatgaacat
    13261 gatctcaatc aactaccagt attggtgaat caaccagagg caacttacgt gtttgatcgt
    13321 gggtattagg actttgaaaa gatggatcag atgcattggg atggctattt cttcgtcact
    13381 cggattaaaa aaaacaccaa agttcacgtg atagatactc tggaaacatc gccagaaaca
    13441 gaaattctac gagacgagct ggttcgttta ggatctaaaa cctatctaac cgccaatttc
    13501 cgactagtca ctgttcaaga taagaacgga cgtgtgtttc aatttatcac caatcgaatg
    13561 gacgtctctt caaaagaaat ctctgatatg tatcacgctc gttggcaaat cgaattgttt
    13621 ttcaagcaca tcaaacaaca catgaccatt aaaaccttct tttctcaaag tgaaaaaggc
    13681 gtgcagaatc aattgatttt aacgatgatt tccgctctcc tcacgtttct catcaaattg
    13741 gagacgaaaa cggaaaaatc agtgttccaa atcaaacgat tctttcgtta tttactgttt
    13801 cagccctttg aatgttgtct agaaaaatta attcccacat gagaaaaaag aaatttggct
    13861 cgttactttt ggttttcagt tgaataacag ataaaaaatt ttctggaaaa agtcatcctc
    13921 tgcgtcagca cagttttttt tgacttattt tcagaggaaa tagttttcag aatattctaa
    13981 aatgttttat gcaacactag tgatattttt tagaattaga tttctaatgt gcattttttg
    14041 agtattaagg agcaagtata caaagattta taaagctgat taaatttgaa taaaaatatg
    14101 caggaacgct actattttta gtagtgttct tttttatatt tgaataaaaa atgtggtggt
    14161 taaatggtgg ttataacaaa aaatgaatta atgaaattag gttatgggaa gtatcagtct
    14221 gagagaatca tacgacaggc aaaagcaatt atggtttcaa aaagctatac ctattacgaa
    14281 aatagaagat taggactagt acctgttgaa tctgtagaag aagttttagg tatgaagata
    14341 gaggtagagg aggattttca gaatcatggt taattttaaa aaagataaaa atgggaaata
    14401 tcattttact gctagtttgg gattcgacaa acgaacaggg aaaagagtac aaaaaaacgt
    14461 agaggggtta aaacagttaa agaagaaaaa aagcgtatac cgaacttgtt tctaattatg
    14521 gaaaagaagc agaaacttct caaagtacta tgttgtttga ggagtttttt tataaattat
    14581 tcctacctta ttatcaaggg aaagtagaag aaagaacata taaaaatcgg ttatctacaa
    14641 tcaaaaaaac ttttcttatt tctttaaaat ggagttaaga gaaatacgtc caattgacat
    14701 tcaaaagtgg caaaataatt tattgagcaa atataaaaat tcttatgtaa gaaacatgta
    14761 tgggattttt cagatgtcat tagatcgagc agttgttttg ggactacttg aaaaaaatcc
    14821 tgcaaaagtt attggaaatg tgaagaaaag gaaaactaca gttgattttt ggacaaaaga
    14881 agaatttaca aaagttattt caacttttta tattgatgat ttgtaccaac attttggttt
    14941 tatttgtatg tggttacttt ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac
    15001 ttggaaagat attgattttg aaaataaaaa cttgcgtgtg aataaaacac tctattataa
    15061 gagagccaaa acctatgagt ttgtagaacc cgaaacggct tctagtattc gtacaatcga
    15121 actagacgac aatacaatta cctttttaaa agcttggaag gagcgtcaag tttcaatagg
    15181 aggcatgtcg tttgtacttt cttataatgg aacacctact cagaaatata caatccgtca
    15241 tattatgaga agacatgcaa cgatcgttgg tgttcatata atccgcatac acggattaag
    15301 gcatagtcat gcttctttgc ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag
    15361 gttaaaacat gctgatgtac aaacgacttt aggaacttat ggtcatttgt atctaaattc
    15421 attgagagag ataacaaatg atcccacaga ttatatccaa actaattttt ctaaaatcaa
    15481 accgacacct atttacgata ataaaaaata aatgataaac aaaagtagga tcacaccaaa
    15541 agaaacacac tagaaaaact ataatacagg cttttctagc gtgttgtttc ttatttccgc
    15601 ctatttatac attatactca taaagcattt aatgcatcga tcgtagccgc attgtctata
    15661 tgggtatata aagttgttgt attggtactt gaatgtccca attgttgggc agttaatacc
    15721 aatgaatttg tctgttggta gagtctagta gctaatgtat gacgcagttt gtgagggctg
    15781 acttgaattc taaagccttt tgaatatttt gctaccatcc gttcgattgc aataccggat
    15841 atacgcttgg cttctccttg gtaaattgct agaaataaag ctttttcgtt ttctgttact
    15901 ttatatcgtt ctttccgaat agctagataa ttagctaaat attccattgc gaatgctgct
    15961 atattgacag catctctttt atttcctttt ctagttacta ccacattatt cttatctaaa
    16021 gataggtcat ctaaattaat atttataacc tcggagagtc tcaatccaga tcctaaaatc
    16081 agtgcattga tcgccaggtc tctttctaca tctcgttgat aatagatcaa agctcgttta
    16141 ctcaaagtat ttttatattc attggcaata taatctagat aattttgtgt atccttatct
    16201 agaaataatt ttttctcaat ggcattggca cgtgcagcta acgtagtttt atctgtgatg
    16261 gttgctactt gatccatagg attattgtct aagtatggtt ttcctagagg attattggaa
    16321 cttttcgaaa gatatttaaa tagaaccttt aatgccgtga tatttctatt gatagtagaa
    16381 gtagatagac ctaagttatt attatctttg agcttagttt ttgttgtctc tttttttaca
    16441 ttttcttttg tgcgacgttt caaatgagat ttgtacaagt ttaggtcata ggcagttaac
    16501 tgtcttatcg aggtgataga agtatcagga acgatagact cactcagaag ccattcataa
    16561 aagagcttat aattttttag atattctaaa gtggttcgag gtgattgatc tttatcctca
    16621 cgatcttgaa caaaatcttt tatataatcg ggcatatgaa gtatgattgt atctattttt
    16681 tgatatattt ctgtattttt catgtcattc atcctttcaa taagtagggg agaagagtat
    16741 ctctataatt ggactatacc aattatagaa tgttttgtca attggtatgc tctaaaattt
    16801 attaatttgt ttcaaaaaaa gcatataaac aggtatacgt ttaataattt aaaataaatg
    16861 gtatacataa atagggaagg aaatagaagg gagcaatcta aattttttga attttcttaa
    16921 ttaattatat agaaagaaca atctttctat agagttagac atgagaggga ctatgcttga
    16981 tttaaaatca acaagcgtat atactgtata tacaaagaaa aaggagggag aagactatgg
    17041 caattaaaga aagaaaattt agggaagtgg ggaactcagt agtattaact atttcaaagg
    17101 agtttcttaa agaaaatggg ttaaatcccg gagatacggt ttttttagat gaagaaaaac
    17161 ttcgagcggc aattacaaaa aaacaagcct tatctgatga aattgattta gcaatatctc
    17221 aagcattaac tgaatatgat actactttta gagagcttgt cgataaatga gtattcatta
    17281 cctagaagcc aaagatatta taaaaatgaa tgtaatccaa ataaaaaaat actctcctaa
    17341 tgaacccctt ggtgtaaagg atgcgaatgc tttagaaatg tgtgtagggc aacttcgagc
    17401 ctcagctttt ggtgaagagg tttatccttc gatttatgaa aaagcagcta tcctacttat
    17461 acaattgata aagaaacatc cttttcacaa tgcaaataaa cgtactgctt tcttagctac
    17521 ttttgtcttt ttgaaaatca atggacagct tttagctatt gatcaaaaag atgctgtaaa
    17581 tttagcggta tatattgcta cgtatgataa ggatttcgat caactaaaat atgaagtgat
    17641 caacactata aagacacata cgcagcccat ttaaggagtt actgtatgtg tctttatagg
    17701 gttattagct gctatagcga aataattgaa gtaaattaca aatagctata attcaaatcc
    17761 aactatatag aaaacgtaaa ttttctatat agttggatta agataacgac tatttatttt
    17821 atcatatttt tactataccc tatactttta cttggtatat ttatgaataa tcgcaaattt
    17881 acccctatac tactagtttg taagttagat taataaaaat actaaagtta tttgttcttt
    17941 tattaaatac acctacaact tataaattga tggtatttag ataacacgaa agaataaaaa
    18001 taacagctga tagtaaagtt tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt
    18061 atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt tacatcttgc agaaatcggt
    18121 actgggatta aactaagcaa taaaaactaa tgttcccctc attctttgaa cttctactat
    18181 ttgatttatt tattctgata aatgacgatt tcaccttcag gatcttcaaa tcccgtatag
    18241 aatttattta agatctcttc agtatcagta ataggatagg cgataggcca catacccttt
    18301 gatggttaga ataaactgct caaaatctgt atcgtatgtt ggctttagca tattaacaaa
    18361 ggctatctcc aaattataaa ccataagcgt tgaatcgttc ttaacaattt caattcttcc
    18421 taacccaaag tcagaagata taattaccgg acttgcctta cccttcatgt gggttgttga
    18481 gattccatat ttaaagggaa ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat
    18541 aaaaaaatct ttcccctgct agtctcctaa gtccctttgt agcggatatg gcaccataat
    18601 ctcattttat cgaagtgagt catggtttat ctgttggcca accaaaattg atacaatcta
    18661 acgatgcacc ctctacggca ttctagggga gtgcacttgc attcacccaa taaaaaccgg
    18721 cagttagcaa agaacttatg tagtcgtatg ttcttactgt taacatacaa gaggtctcct
    18781 ttccacaatt gcttagcagt aaacgggaat ttacaaagct aatctcttcc attttcttat
    18841 ctttgttcta aaagtcccaa atggagcaac tgtatttaca tgaataaatt tatatacttc
    18901 ccatacagcc gttttggttg cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc
    18961 ttcatcactc attgaatcaa tcatcatgtt aatatcatca atattacgat tgagaatttc
    19021 ctctaattct ttcaatggta gatgtgcata ggtatttgta aaccacttat ataactctcc
    19081 taactgattc catttaaatt gatcagaagg cgttttaaca gataatccat ttctttcatc
    19141 attttcccat tttagaatta aattcgtcca accaacttga tatgctaaat tttcagcagg
    19201 tgttctatcc acttcctcaa ctcttttatc cttcaattct tcaggaatag tttcaaattc
    19261 taaaatatat ttagtataag tcctatttat ttcttttttt aattcttctt tatttttata
    19321 tgttctcatt atattacctc cataaattcc taattgtctc ctttaattca tctataattt
    19381 catccacgat attttaatcc gttctacaat gcatatctga tatgcattga ctgtctaact
    19441 catatgtgaa tgatttataa aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag
    19501 caaggagcta aaaaatacga atatatttct tttgtagtaa aatcggactg gtttgcatat
    19561 cattttcttc gtaaatatca aaatcatgtt aactactatc aattactaat aaaaactacc
    19621 actttaagcg ttaagaactg ttaagtttag ttaagtgagg atagttcaca aaatccattg
    19681 ttaaacttca cgaaagtggt aaaactcaaa atgctttata taggattaca gcatcgctct
    19741 ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa aaattgacga tgacacgttc
    19801 cttacggctc atcagatgaa acagctacaa aaacgaaatg atcaactcga agaaaattta
    19861 attctaaaaa agcgattgcc atattcacgc ctcgctcgac gaactattaa aggtcaagat
    19921 ttacctgaag atcatccttt ttaggaaatt gaagcttttt atgttgcaca atgcatttat
    19981 aatactacaa tgatgttttc accagatatt cttctagggt gcggtgtaat gaaacagata
    20041 cacttgcaaa agaaaattca ccgcgaattt actaaaatta tgaataatta tgttaatctt
    20101 ccgaaatttt tttccaaaat taagggatca agctggtgtt atagattgtt taaggttggc
    20161 aaaaaaataa cttcttttta aacaattata gaaaaccgat actctgttaa gttggttgac
    20221 acggtgtcga tttttatgac tttcttattc tgaaacttca ctaacggaca gtacctaaaa
    20281 agtctatagt atggacgttt acgagtacta ttttatcaaa aatgctgctc atttcaactt
    20341 atatgtttta gtgaactacc cccacttagc ttggataggc tacaattaac tagacagaaa
    20401 aattaaggtg tgtagactag aagaaaacat accaggagga atttttatgt ctaagagaac
    20461 acgaagaact ttttcacaag aattcaagca acaaatcgtc aatctttact tagctggaaa
    20521 gccacgtgta gaaatcattc gagaatatga actaac
//
LOCUS       DACDRK010000041        20425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDRK010000041 DACDRK010000000
VERSION     DACDRK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.041"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(147..887)
                     /gene="truA"
                     /locus_tag="IWJ77_001674"
     CDS             complement(147..887)
                     /gene="truA"
                     /locus_tag="IWJ77_001674"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9453278.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1078..1713)
                     /locus_tag="IWJ77_001675"
     CDS             complement(1078..1713)
                     /locus_tag="IWJ77_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9453279.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(1860..3101)
                     /gene="sstT"
                     /locus_tag="IWJ77_001676"
     CDS             complement(1860..3101)
                     /gene="sstT"
                     /locus_tag="IWJ77_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9453280.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3299..4297
                     /locus_tag="IWJ77_001677"
     CDS             3299..4297
                     /locus_tag="IWJ77_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453281.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4310..6328
                     /locus_tag="IWJ77_001678"
     CDS             4310..6328
                     /locus_tag="IWJ77_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9453282.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6388..7104)
                     /locus_tag="IWJ77_001679"
     CDS             complement(6388..7104)
                     /locus_tag="IWJ77_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9453283.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7331..7873)
                     /locus_tag="IWJ77_001680"
     CDS             complement(7331..7873)
                     /locus_tag="IWJ77_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9453284.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7878..8105)
                     /locus_tag="IWJ77_001681"
     CDS             complement(7878..8105)
                     /locus_tag="IWJ77_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9453285.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8184..10064)
                     /locus_tag="IWJ77_001682"
     CDS             complement(8184..10064)
                     /locus_tag="IWJ77_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9453286.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10253..10867)
                     /locus_tag="IWJ77_001683"
     CDS             complement(10253..10867)
                     /locus_tag="IWJ77_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453287.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            11009..11569
                     /locus_tag="IWJ77_001684"
     CDS             11009..11569
                     /locus_tag="IWJ77_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9453288.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(11874..12188)
                     /locus_tag="IWJ77_001685"
                     /pseudo
     CDS             complement(11874..12188)
                     /locus_tag="IWJ77_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(12442..14077)
                     /locus_tag="IWJ77_001686"
                     /pseudo
     CDS             complement(12442..14077)
                     /locus_tag="IWJ77_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14632..15195)
                     /locus_tag="IWJ77_001687"
     CDS             complement(14632..15195)
                     /locus_tag="IWJ77_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9453289.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(15210..16253)
                     /locus_tag="IWJ77_001688"
     CDS             complement(15210..16253)
                     /locus_tag="IWJ77_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453290.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(16386..16790)
                     /locus_tag="IWJ77_001689"
     CDS             complement(16386..16790)
                     /locus_tag="IWJ77_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9453291.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(17023..17658)
                     /locus_tag="IWJ77_001690"
     CDS             complement(17023..17658)
                     /locus_tag="IWJ77_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9453292.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(17924..18976)
                     /locus_tag="IWJ77_001691"
     CDS             complement(17924..18976)
                     /locus_tag="IWJ77_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9453293.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(18989..19927)
                     /locus_tag="IWJ77_001692"
     CDS             complement(18989..19927)
                     /locus_tag="IWJ77_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9453294.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         6457 a         4067 c         3251 g         6650 t
ORIGIN      
        1 agatttttat ttaagaaaga ctttttagct gtacacataa acaggctaaa aagtcttttt
       61 ttatatttct ttaatttgct tcatacatta tttaacatcg atatttatac tgtgaaacat
      121 gcgtttcatc cattatcaaa gtatttttat cgataataaa cttcatccaa aaacagacct
      181 tgtgcaggga cggtttctcc tgcatgttgc cgcactttgc tttcaaatat ttcatcgatt
      241 gtttctatcg gcatggcacc tgtgccgatt tctagtaatg tccccatgat aatccgcacc
      301 attttatata agaatccgtc tccgacaaaa gtaaaatgaa gcatttctcc ctttcgttcg
      361 atagataatt catcaatcgt acgcactgtg gatttttttg attttttcaa tgcagaaaaa
      421 ccaatgaaat catgtgtacc cactaatttt tcgcaagctg cattcagctt gtccatatcc
      481 aactcttgag ggaaataaaa gctgtggttt cgctcaaatg ctgaaggaat cacgttgttc
      541 caaacataat agctgtattt tttgcccacc gcattatatc ttgaatggaa gcgctcaggc
      601 atttcttcga ttcttttgat gacaatatcc cgcggaaggt aacgattcat aaagtcgatc
      661 atttctgcca gtccaatttc tgtgtttgtt ttaaaatttg ctacttgtcc tcgcgcatgg
      721 gtgccagcat ctgttcttcc tgatccaata atttctatct cttcatttgt catctgatgc
      781 aatacttttt cgatttttcc ttgaatggtt ttatcgtcat ctccaagtct ttgccatcct
      841 gaatatcttt ttccgtcata ttcgattgtc aacttgatgt ttctcatatt ttagtggctc
      901 ctctgtcttc catttggtct tctctctacc tttttaagat tgtagcacaa aatatagctc
      961 cttgcttctc taagacagaa gctatacaag aaaactgtgg aaatatatat ttcagaaaaa
     1021 caaaaaacag ccaccggatg aagtatccgc tgactgttca atttgacggc tcaagtttta
     1081 ctgtttgaga tgcaagatac tcaacctggc aatgggcaca taattaaaga aaaaactgat
     1141 tctttggcaa aaacctattt tattcagtaa tggccaagaa tttatagaag gaattcttgc
     1201 cagtccataa atcgccgcaa aaagcaaact gatcacagac agtccaaggt ataagtggct
     1261 tacgtccatt tgcccatttt ttcgatagat caagaaaaga agaactggaa acaataaaaa
     1321 accactgtaa cctaaacctg atccgatatt atggacccat gtcgagacat tccacgaaga
     1381 ttcatgggta ttgatactga acaaacctgt aaagatacaa tcacctatac catagaacgc
     1441 gatggctgct gtaactaggg tagctaacac acgagatgta ctaacaaatg cagcatacaa
     1501 tgcaggcaat gacaggatga aaaagatccc tgaaaccaca gaccaaataa gaaacgcttt
     1561 tcttacagga ctggaaacct ctccaaatac actgatcacg ctcttcattt gatctaattc
     1621 aggataatac aatcctaata cgtacggagt caaaaaatca ctcagcacgc ctaataataa
     1681 aaagaaaaaa ccaaatttac gtaaaaaatc catgatacgc ctccaataac aattacctat
     1741 gcttttaatt gttattataa acgactttat cttgatttaa cagaaacctc tatttacacg
     1801 aacatctata taactcatta aaaactcgaa tcattttaaa aatactaaat atctaacgtt
     1861 caacgttcac ttaatccatc taaacaatct ttcaatttga ttttttcccc acttctttta
     1921 cgcgcgccta gctctcctat tgcagtaaac aacagatcac ttgacgaatt taaggcagtt
     1981 tccattgaat cctggataac accgataata aatccaatac cgacgatttg catcgcgaca
     2041 tcattagaga taccaaacaa actacatgct aatggaatca ataacagtga accgcccgca
     2101 acacctgaag ctccacaagc agctaaagca gacaaaatac ataaaaggac tttcaaaaca
     2161 aaaggtgctt caatccctaa agaatgtaca gcagtcaacg tcatcaagga aatggtaatc
     2221 gcggcaccgc ccatattgat cgtagcacct aacgggatcg atatggaata agactctttt
     2281 gtcaatttca atctttcagc caacatcatg tttatcggga tatttgcggc agaacttcgg
     2341 gtaaagaaag cgggaagtgc gctttctttc aaacaaaaga aaatcaatgg atagggattt
     2401 tctttaataa acaaaaacgt gaggaatggg tacacaacta atgcgacaaa cagcatcgta
     2461 ccgatcaaaa caagtaaaag ctgcagatac tctgccaatc cagcgattcc agtcgtagca
     2521 attgattgat aaaccaatcc taaaatccct atcggagcaa atgcaataat cacttgtacg
     2581 acttgtgaca acgctataga tatctgatcc accaactctt ttgtcgattc tgaacgtgct
     2641 cgaaaagcta gtcccaacaa tacagcccag aataagacgc caaggtaatt cgcatcaata
     2701 agagattgaa caggattttg tgtgacattc gtcaacatag tagataaaac actttccaaa
     2761 ctttgcggtg ctttttcaga tactgtttct tgtaaagtaa taggaacagt aaaaattgaa
     2821 cttacaataa ctgctacaag tgcagaaagc aatgttccca ctaaatataa gatcaaaata
     2881 ggcttcacga atgtcttatt cccaatttta tgcttggcaa ttgacgccat cgtcaagaaa
     2941 aaaaccagaa taggtgcgat tgccttcaag caactgacaa acaacgttcc caatattgaa
     3001 ataaatgtcc atgtggggaa aaagatacca aacaatattc cgactaaaat accaacaaca
     3061 atacgttgaa ctaacgacat atccaaaaac ttctctaaca aatgaccacc ctttcacaat
     3121 tttaacaatc attgtatcat tttatgcaaa gaaactaaag gtataatttt ctaaaaattc
     3181 taacaataca aacaaccatt tcttgctaat tttgtacttt ttctgtctac acgttgtttt
     3241 ttgagatatt ctaagttagg atgttattaa tgggcaactt atcaggaaac gttttcttat
     3301 gtttttgtcc aatttttttg tgaggtgttt attcttgaaa aaaataaaga aggagagtta
     3361 tggcttcctg acaatctttg tcgcttgtat aatgatggtt ttacagcgta tattgatttc
     3421 agcagtcact attcctacat atgcaacaaa ccctggtcga ttctacatct atacattttt
     3481 acaaacttcc gttgttattg gtgcaaattt gatcccactt tacatgggtt accaatatca
     3541 aaaaatcgcc cgtttaaaag ttttacatta tttaagtcgt tttgccttta tattcctaat
     3601 agccacacta gtttgcaata tcttttttta cgtacaagct ggcagcttga atattcgtga
     3661 ttattggctt atcctaacac ctatcagtca gaattacttt acttatgctg tttcttgtac
     3721 tttgcttttt tgtagtattc cttatacagt tggattttta aataaattat cgaaaacaac
     3781 aattaaatat ctactattat ttttaacaat tggattagtt ggctgctcta ctctatttaa
     3841 taaagacccg tttgcattac agaatggaca gagtatttta tggatttttt atttattttt
     3901 aatgggttat ggtttgaaag aatggaactg gaaagaaaag gtccgctata agcttcctca
     3961 agtatttatc agcttgattg ttttattcgg tttgattatt ttgatgacac aaatttcact
     4021 catcatcaga ggaaatacag atacagcgct gaggttttct aaaccatatt ctctcttcgc
     4081 aatgtattat actttctctt cgtttttatt tttagaaatg ctatctgata aattaggtat
     4141 aaaaggaaga ggaaattttc ttgctgtatt tttgattgtc actcaagtta tatgtaattt
     4201 ccctttagtt tcttatcgcg tatctacatt tatgaaacgt gaatttccaa actcagggaa
     4261 ggcatggctg ataaatatcg ggcagttttt tatttgatat ttattagcag tgtccctcct
     4321 agttatcttt ttattttata tacaaaaaat ccgtttcatt catcgtttca tcgaatactt
     4381 cgcttttgat tcgattgaac aattaactca gcgttggaaa aaaagaattc attggctttt
     4441 tgttcataaa agttattttt tagttgcagc atttttttac tgctttacct tcgtgcagat
     4501 tttcgtttta gaaccaaaag aaggttggaa agaaactatc caggttgcct taaaaatatt
     4561 tactcagcga caagcaccca tgatcctgac aatcatcatt attatgggat ttttcttctt
     4621 actattgttg atttccaatc gtttttggta cgccttgact gctaccttga tcattaactt
     4681 actgctcact atttcgacag taatcaaaat ggaaatgcgg gaagagcctg tctttccttc
     4741 agatttaaaa atgctgaccg gattgtcaga actattatcg atggttagcc cagtcttatt
     4801 aatcgtgggc ggattgatcc ttctcctatt attgattact agtattattg tacaacgtag
     4861 actacaaaaa cagtattcct tgaagattca ttggaaacgt cgatttatca gtatcgctgt
     4921 attattagga tgtttttcgg gtgtcttttt cattaatcac aaaaattcgc cttctttctt
     4981 gctgtttaat ttatttaaag taaacaagac tttctttaac cagcaaggcg ccgtaaaaga
     5041 taacggacca gtcattcaat tcctaaacaa tatcgacatt aaaatcatgg aagaacctgc
     5101 tggctattcg aaagaaaaaa ttaatggaat aatgaaaaag tacaacacaa aggcagatga
     5161 aatcaaccaa acaagaagtg actggttaaa caatcagacg atcgttctca atttaagtga
     5221 aagcttcagt gatcctgcac gtgtccctaa tctgacagta cctacagacc ctattcctta
     5281 catcaaaagc attataacag aaggaacagg aggattgatg ttatccgtcg gttatggtgg
     5341 cggaacagct aacatggaat gggaaggact gacaggcttg agtatcagta acctgtctgc
     5401 ttcgttagtt acaccttata cccagctagt ggagcgtcag aaaataagtc caaatattac
     5461 cgatctattc gatgaaaaaa tagccatcca tccttttaca gctgctcttt atcgcagaaa
     5521 acaagtgttt gataaatttg gttttgacag gttctattat ttagacagtc cggataaatt
     5581 aacttatacg gataaaatag aacaaagccc acgcatatcg gatgattcag catacaaaga
     5641 aaccttgaaa gctttacaaa gcaatacgga aacgtcacaa ttcatccaat tatccactat
     5701 gcaaaaccat atgccttatg atgattacta tagtgaattg aactacactg ctgaaggaac
     5761 tgctgtcatt cctaatagaa gaaaagaatt acaaacttat atgcaaggcc ttcactatac
     5821 cgatacagca gtaaaaaaat tcatccaaga aatcgacaaa atcgataaac cgattacctt
     5881 tgttttttat ggtgaccatt tacccgcaat ctatagtggg aatgattcga aaaaatatgg
     5941 attagaacag caccagacag attatttcat ttataacaac aagtatagtc gtgaacaagc
     6001 agctacgtta aataaagaag ttgttgctcc taataacttt ggtgctatgg ctcttgagca
     6061 tgcgaatatc aaagtaactc cttactatgc gttgttgaca gaagtatcta atcatctacc
     6121 tgcattaaca attgatccta cagaaagctt gtctaatcgt tttaatggaa aacaggtctt
     6181 tgttacagag gatagtcaaa ttatccaaga aaaaaattta actgagaaac aaaaagaaat
     6241 tctagaagat tatcgtctga tacaatatga tcttgttgca ggtaaccagt acagtgcaac
     6301 atgggcgaca caaaaaataa aagactaaca aataaactga gcaaggggtt gtgataaaag
     6361 acatgtcaca accccttgtt tatctcatta atttgcttgt ccataaacga tttgattaaa
     6421 gccatttttc aattcattat tcgctgtctc gttgaagtaa agataattat cgggattccc
     6481 ctgattttgt tgtgtcacca atacttttgg ctgattttgc tgaaacccga atagatagac
     6541 gtgctttttc agatatggtt ctgtctctgc atcggaatag acggctgtga tttgatagtc
     6601 tgcttgacct gtcccacttt ctgaccattc tgcagaaaca ggcactccac caatagccat
     6661 cttccactcc ccgttcaaaa tagcttgagg gactttaaga ccatataagt ccacactcat
     6721 ttgattcgta taacttttgt attgttgtcc cattgtttgg ccccatttgg tcataaatgc
     6781 ctctaattgt gcggatttgt ctgcattcca cagtgtttgt tcaacttgtt cgctgctttc
     6841 tgaagaggaa gatgaagcct ctacagaaga acttgaagta gatgtttgag ttgtttcagt
     6901 cgttttggaa gactctgacg aagtagaggt atctgctgtt tctgtacttg ctgcagttga
     6961 cttgctctct gtgctagttg ttgtagtagt tgcaactgtt gctttcgctg tttcttttgt
     7021 tgattctttt ttcgattcat tcccgttggt acaaccagct aacaaaaaag ctagaccaat
     7081 tgttactaaa atcctttttt tcaattaaaa aactcctttc gattactccc acttctttat
     7141 tctatcaaat aagcaatgtg attttattga tttttcgtat tttctcatca atctttcaaa
     7201 tcttctaaaa actgagaagc acccgctgat gttgtctgta atacatcagc gggtgcttct
     7261 tttattttaa tcttcgtgat tttccaattt acctcacgct ttatatttgt atttgaagaa
     7321 acatagactt ttagtcatca tcgtcttcct catccaaatc ttgtcggaca gtttttcctt
     7381 ggagtctttg tacttggtag atgatttctt tattgaaact taaaagttca acaagcaatt
     7441 gttgcaagcc atatttttct tctttttcag ctaatttgat tgcccgtgtg ataaagacat
     7501 tttggaaatc aaaatctttt gcaaagtttt ccaacattgt tgaggctggt aaatatttgt
     7561 tttgaccatg ttcgtaaatt tttccataac gcatgacttc ttctaaagta gacggaacga
     7621 tttcattttc aatcactaac tcatgagaga ttttattgaa ccatctcgct tcttgatcag
     7681 catattctga aaaaagggta cttaattctt gcgaagatgt acccaaaaca tacaattgtg
     7741 cttggatcaa tttcatttct tctaatttaa aatttgctaa tacatgcccc accatggcgc
     7801 cagcagttgg tttatgatgg tcgatatctg cttgttttgt ttcttgaaca aatttttctt
     7861 ccactatatt cattgattta cgcctctttt acttttgctg attggacttc ataacctaat
     7921 ttttccacaa ctgcagctaa ttgttcaggg tggacttcat ctggatcaaa gtttgctttg
     7981 attttgctcg cattaaacaa aactttcgta ttgcttaccc caggctgttg agaaaccgca
     8041 tgttcgattt tttgcataca gcttggacaa gctagtgtat ctaattgcat aataattttt
     8101 ttcatcgtag ttcttccttc ctttttcttg atagtcttat tctagattac agcttccata
     8161 aaaaaattga ttcttatcaa gttttacgat ttttcttctt tgctttcgtg tccaaaatac
     8221 aataaacgca tcgcgttaaa gataacgatc aagatactag cttcgtgaac gaacatccca
     8281 ctagccatgt aaataaaccc ggcaaataag ccgttgagta aaaataagac gacaccaatg
     8341 gcaatcacta cattttcacg ggtatttaaa acagttttct tagccaagcg gtaggcatga
     8401 accaacgatt caaaattaga ttgcatcaag acaacgtcac tcgtttcgat agcaacatcc
     8461 gttcctgatc ccatagcaat cccgatatca gccgcagcca aagagggaga atcattgatt
     8521 ccatctccaa taaaagcaac gcgcaatcct tgttcttggt attttttcac aaattgaacc
     8581 ttttcgtctg gtaataattc agcatggact tcatcaatgc ctaacatatc agctacataa
     8641 tcagcagtca tctgattatc acctgtcagc atcactaact gttttgctcc tgcttgtttc
     8701 aacttagcta attgttcagc gacttctggt cgaatcacat ctgacacacc tatgatttga
     8761 gtgatttttt tgtcgattgc tacaataatg accgagctgc ctgtttgttg taattgatgt
     8821 aaatcttgta cttgctttgg tgtgagagaa agtttgtgag cggcaatcaa tttggtgttg
     8881 cccgcacaaa ctgtatgacc atcaatctca gctgtgattc cttgtccctt gattgtctga
     8941 ttatctttga ctgctaattg ttgaaaatca atgtctttct cctgagcata atggatcacg
     9001 gcgcgcccca gaggatgatc ggataacgtt tctaatcgag ccactaaagc taagctgtcc
     9061 ccttcatcct ctgtatagtt cttgatagtg gatacagcag tagttccttt ggtcaatgta
     9121 ccagttttat caaagacaaa agtatcgatt ttggaaaaag tatccattgc ttcaccgcct
     9181 ttgactaaga tgccccgttt cgctccatta ccgattcctg ctacattcga aacaggcgca
     9241 ccaatgacta aagcgccagg acaaccaaga accaaaacag tgattgctaa acgaaaatca
     9301 cgactaatca gaccaacaac caacgcaata agtaaaacgg ttggagtata atactgagaa
     9361 aatcgatcaa tgaatttttc cgcatgggat ttggtatctt gtgcttcttc tactaattcg
     9421 ataattttgg caaaggtcgt atcatctccg accttagttg cttcaatcgt cacgtagcca
     9481 tctgctaaaa tcgttccaga gaagacttca tctccactga ttttgacgac ttgtcgtgat
     9541 tcaccagtca ctgcggcttc atccgcatac cctttcccgt caatgactcg tccgtccaca
     9601 ggaatagctc caccagtttt tactaagacg atatcgcctt cttccacgtc atcgatttcc
     9661 acttcttcag tcgttccatc ttcttggaca acactagcag tagatggggc catctcggtc
     9721 aaagccttga ttgactcccg cgttttggtc aatgtttttt gttccagata gctaccaaac
     9781 aaaaacaaga atgtaacgat agctgactcc ttgtattctc cgatcataaa ggcaccaagc
     9841 acagcaatag taacgagtaa atcaattgag ataactttga ctttcactgc ctgatacgca
     9901 tggatcataa taggtatgac cgcaataata gaagctatgg ctagagcaat gttatagaac
     9961 aagacaaaac cagtaaattt tccgataaaa ccaaaaataa tcaacacagc tgataaggcg
    10021 gtgatatgat ttcgatgctc ttggatccac ttttgtaatt tcatctttct ccctcttttc
    10081 ctttcattct tacgagacta agtatagata cttatccatc ataaaaaatt gaccactatc
    10141 aagtttagtc aaaaaggcgg ttttcttttg gtagaataca ctacaaaaaa tagttagcaa
    10201 gaagactcac taaaagtgat cttgccaact attgtcgttt ctatcgaaat tttcattgaa
    10261 tgattttcct gtacagcata catcggagat ctatttcttt atctaagatt tttatcttgc
    10321 taattttttc ctttagtctt tcaaatccac gtcgttcata gaattgatac gtacagccac
    10381 tatctgtaaa aaggaatatt tcttttcctt tctctctttt ttcaaattct gttagcaact
    10441 tggtaccgat gccttttgtt ttcaaatcag gatttgcagc taaaaaaatg atctctccat
    10501 ctggcgaatg cttctttaaa tatgctgcaa gcatttgttc atttgctgtc tcgtaagctc
    10561 ctttactttc tttagcaaat atattttgta aaaaatcgaa tatcttaaca tatatttgct
    10621 gtccaaaaga acgatgtttc tttttttctc cttttatctc cgccagcaaa acacctgcca
    10681 actcgtcctc tgcatacaaa gcaagaattt gagttgctcg attcaactcc atatacaaaa
    10741 aatatcttcc gtaaagattc agcagaagtt cattttccat atatctctca aagttcattc
    10801 cttcaacagc aaaatagata gcttttttgt aatctttctt ttctaagggt ttaaaagtta
    10861 gtttcatcat ttcgcctcct caacttcaat gattgtgtta tacttacatt ataatagaca
    10921 cagtgtctac tatctataat caatcatcta taagtttgtc gattaagcga cagctttgct
    10981 ttgatatgtc ggataaggag ataggtaaat gaaaaagaca gatagacgcg tcaaaaaaac
    11041 agaaaaagct ttggctgaaa cactatccac attattagta aataaaaaga ttcaagcaat
    11101 cactatacgc gaactaacag aaacggcaga tgtacatcgt tcgacttttt atacacatta
    11161 taaggatatc tatgatctat atgatcaatt agaaagcaac ttttttcggg atctaaatga
    11221 aattctctct tatgatccag cccatagcta tgaggaacta tacactcgct tgattgacta
    11281 tgtttacgca aatacttttt tatatcgctt atttatcctc aaccatggtg atatttcgtt
    11341 tcagaagaaa gttaaacaag ttattgaaga aaactatctg aacatatggc tatatgaaga
    11401 caagaaaacg attatcacag aagaaatgag attctttact acgtaccacg tccaaggttg
    11461 tctatccgtt ctcaatcggt gggtagaaag caattacaag tactccaaaa aagaattatt
    11521 agaactctta cagaaactga atgatcacat agagaaaatc atgccttgaa taaaaacata
    11581 ctaagcttag ttaagaagaa ccgtaacctt ctcgtataac aactgaatct tatttagcca
    11641 aattgtttac tgaagatttt ttgagtaaaa tttctataat tcctacttat tgaaaagtag
    11701 tttgtagtgc tgcttctccc tctctaagtg atatatcttg ttgtaccaat caaattcacc
    11761 tcatcattaa taaattactt ttaggctaat ttattacaac ttataggttg aagctgtaac
    11821 caatttatag tcatattata tttttatcat ccagtttatt gaattcttct cgtttacctc
    11881 aattcatttt caaggggaat cagctcatca tctaacattt tacgccatgc tcgcttcatt
    11941 tcttgtgggt atttaaatgg tttcgcttct ggatgtttgg ctttatatcc ttcgtaatcc
    12001 ttactgttga taattgcttt catattagga attggatctt cacctggcat atattgcaga
    12061 ttagaagcat gtaatttttt ctcagaaaca aaccactcat tggaaaagac atgaatcaaa
    12121 tcatcactta cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attgctatct
    12181 agtgtacctt catttctttg tgcttaaatt caaagataat tttctcaata aagatttttg
    12241 attccagtaa gattccttta ctccttttac aacatagcgt tgtcctttgt tttgaaacat
    12301 cttcttcccc tctttgccat taaaaatagc ccaattctct ataaagaaga ttaagcttca
    12361 ttattataat ataaagttta aatttggttt attacttact ttttcttatt ctctagtaag
    12421 ctaatattga aaaaaatgta atcagatcat tatttttata tgttgtaact tcctgataag
    12481 aaaactaatt aaaagaaaga ggtaactaca taaactaatt aaaaaagcta acttcatagt
    12541 tggaggataa atggaagaaa cttcaatgat tgtttctcct tgtgttcctt caatcgacac
    12601 tagtcctatt atcaatctct cttttttgtc catttacttt tgcgactgtt ccccaataat
    12661 caaatacagg aagcgttatt ttttgtcctt tagattcatg tactgtatag actattttcg
    12721 aatccatcac ttgataaggc atagtatgcc attccttgcc aattcttact tcatgattct
    12781 gcaaatgttc tttgtattgt aatgcttccg tgggtgcata gtcataaagt cctccgggaa
    12841 ggagtgtcgt gtaacgaata agattttctt gattcattac caagtgatct ctctgagtga
    12901 caactttatg ttcatagttt gcttttgttg cagcagatac agccaacagt aataaaacaa
    12961 ttgccgttat attaactact tttctttgct tagctgatgt gctcttcatg aaaacgaacg
    13021 ataaaatcaa cgagccaaac agtacttgaa agcctaagat tcgccaagga aattgaatca
    13081 tttgaatggg tgtattttgt aataaagacc atggaaaaaa tttggtggat aagagccaag
    13141 agactaccgc actaaggtaa atcacttttt ctttgcgatt aaatccttta aaaaagaaga
    13201 gtggacagat caaagaaata ataccaatga ttcctaccgt aaaggttgtc aggtcattat
    13261 tcatcgcacc aatcaaacta tccgagacat ttaatgcttg gtgttgtaag gtcgttttat
    13321 ctggctcatt aatcgattga accatgattt gtcggaacat cggataccaa aagtatgctg
    13381 ttaaaagaat cgtcattcct actgcctttg acaactccac aatcaaccgg atgctttctt
    13441 tttttcttgt ttcacttact acaaagccgc ctaagactag taagccaacg gctattacac
    13501 tcataaaagt ggaaagaaca tgtgtataaa tgagtaagct catactgact gctaatggaa
    13561 ttgcacatgg ctttttctca taaatcagtg cgtaagcata atacataact atcggtataa
    13621 ccgtaagtgc aatcgcttca gcaatagcag aacgatgata aatatcaatc gtacggtacc
    13681 cataaaatgt atataaacag ctaaatataa aagccgcatc gattcttttt aaaaatttta
    13741 gtccataatg aaacatcagc atgatagtac aaacattcaa tagccaaaca tataagatat
    13801 aactaacgat taaattatta cttatccaat aaaaaatgac tgccggaaaa aaggtcaagt
    13861 agggataaaa ataattcact ccgctcccat aatgattaaa cgtcgtaaaa ttgattggac
    13921 cagaaaaaat tgaagataac cctttgactc tagcaaaatg gaaggcaata tcccccgatg
    13981 tagaaatagt atgaacattt ctaaaataat tgattgaata aagtccagca ataattgcta
    14041 ggagaatgga caatattatt tttttcttta cttgcattac ttaattatat actcctttca
    14101 tggattgctt tatctagtat aacttaaatt ttaaataaat cgttatttat ttttactctc
    14161 acttaaaaaa gcactaaaat tataagagag ggaggtaatg gtctgaacac caagaattgt
    14221 tccaacatcg aattgcttaa ctaatgagtt caaagcttaa ttatgtggct cttatattta
    14281 ccgagcgtgg attgcgatct ctttagcgaa acaaacatct ttcataaaaa aaacagccat
    14341 atccgctttc ttggatatga ctatttacta ctactttact atctttcatt cttcttgtct
    14401 cacttatgat aaggcgctgc gtatcatgag tgaatgatat taggaaaact tcataaaata
    14461 aatcttttat acatttccac ttctagatgg tcattcatgt tagaagaatc aaactatttt
    14521 tttactgtcg taataatatt aaaacactag aacaaatcaa ttaaattcca taaaatataa
    14581 ataatagacc tagcatcatc tggtataaaa tggatgctag gtccgttata tttactcaaa
    14641 attgattcta cgaatagatc ttcttatcat ttctatttta gtagtcatgc gttgttgttc
    14701 ttctaaaggt ggtaatggta taagtagttt agataaattt gtaccattta catttggttg
    14761 cccagttccg taagaaattt tttctaataa cttccaatat aatggcgagt ctaagaagca
    14821 gtctacgtat tctgataata atttctcaac taacctaatt cgaatcaagt aagaagcaaa
    14881 aacagattcc tcacttattt ccttaaccaa gaaacttttt cccatagttc ctcctgtcct
    14941 tgctatcaaa atgtcatttt cttccaatct taaatcaact agtttagaat tactaatatc
    15001 acagtaaggc acactggacc aatttacacg tccctcttgt atatctgtga ttctcaaaaa
    15061 cttaacattg ccttgttttt tcgccgaatc tgtatagcca tattgaatct tagtagaaat
    15121 acttcctaaa taaacccata gtcgaacatc gctagatgat ttttcttgtc gttctttatg
    15181 aacaacagtg atcatcttga gttgtccgac tattttttag ttgcagcaat ctttttggga
    15241 cgcttatttg catgaagcaa cgttgctaag tctagtaata aaacattggc taacacagga
    15301 ttggctttct cgcgatacgc tgcggcgcca ctttcaaaca atactgctag acgatcttct
    15361 acaacaacga tttgttccaa atacggtgga aatttgattc gttttaggat ccggctagga
    15421 gagtcccgct cggacactaa gagttccgag gtataaggac cataagaacg actggctaag
    15481 agataatggc cataaaaagc cacaccttga atcttatcag atgtttttct gctcattgtc
    15541 acggttggtt ggattttgtc tttgcggcga attgctgttt tcatttcagc tatcgtcggc
    15601 aatagataga aagtcaactg tccttcacct tttagtgtga agttgccaga taccagttga
    15661 tcattgtttt gagtcaaata cgatgcttgt ggaatctctg agagatcagt tgtcagccaa
    15721 taagcgattg gctgtcccgt ctcttcagat gtatctgcta agatatcgat caaacgtaaa
    15781 gcggctactc gtccagtagc agatcctgtg attgtcaacc ataaaagttc ccgttttgta
    15841 tcatatacta agccccctac atgtggccgt ttaggtaaaa tcagcagttt gatcggctgt
    15901 cctgtttctc tatccagtac atgaataata gaatgatgct gatggtcata gcagtaggca
    15961 gaaataaaca caaaatcttg agaaaaagcc aacccttgcg gcgtcatcca ttgacaaacc
    16021 tcattttcgc cggtttccgg caccaccatt cttgtttctg ttaatccagg aatgttatat
    16081 tgttttgctt cagccttttc ccaagctgct tgaacaatag gataccgttt taactttttt
    16141 tcaatatcct gatgtatccg ttgctgcttc caatgcttaa actttaggac atagtaaaca
    16201 agaaggccaa tgaccagcaa cactaaaatc attattgcta cccgctgtgt catttttttg
    16261 cctccttgtt ctattcgatc aatcgtccat ataccgctat taattaccgt aactacatgt
    16321 ttcttttact agcgtttctt tataggatct ttcataatct catttatagt aacattaaga
    16381 atcccttatt ctactatagt cacttccaca tcaatattgc ctcttgttgc tttagagtat
    16441 gggcaaaatg cgtgggcttc ttctgcgatt ttctctgctt ggtccttctc aagtcctggc
    16501 aaagaaactt ctaaaacgac cccgatttgg tagcccccat cttctaattg aaataaagat
    16561 acccgtgctt tgacttcaga atcagactca agattatttt cttccagcat gtgttgaacc
    16621 gcgccgttga agcaagatgc gtatccagcc gcaaaaagtt gttccggatt tgtcccttca
    16681 ctatggattc ctgggcggtc gatcttcatg tgcatacttc cgtttggtgc ctcaacttcg
    16741 ccatcacggc ctccacgatt gatcattgct gtttcgtaca tttttttcat tgagatcatc
    16801 ctttctttcg tcgttctata ttaagtgtaa cgagtatttt ttaatctgac aaaggtaatg
    16861 cttgcattcg gcgacatcag ttagacaaca tcgatgatca gtgatagaag cagtttatct
    16921 atacaaaaaa agaagggttc tatgatattt ggtgtcctta ctcgccttga gtaacaacac
    16981 caaatatcat agaaccactt aatcagcttc acactgcttt ttctactttc gtagttgaaa
    17041 aaaacttaaa caaatcatcg gcaaatagtt aaagaaaaaa cttaatcgtt gaaagaagcc
    17101 caattgctta aagaaggcaa actgactaat cgacggaatc cgtgctaatc cgtaaagacc
    17161 tgcaattagt aaactaataa ctgttaaaac aagataaaaa tggctaagcg taccttgacc
    17221 actttgacga taaagcagaa caagtaacaa tgggaaaagt agaaagcctg catagcctag
    17281 cccagaacct atattatgga tccaagtcga tagattacaa gagctttcat tcgtattgat
    17341 actgaataag ccagtaaaaa tacaatcacc tatcccatat aaaccaactg ttgcaacaac
    17401 aagtattgca agtgtttttg atgttccaac aaataggtga tacaacgcag gcaacgaaag
    17461 aacaaaaaaa agtccagata caactgacca gaccaagaaa gcacgtctga ctggactatc
    17521 tacgtcacca aacacactaa ttacggtagt catttgattc agtttgggat aaaaaaggcc
    17581 aaggatatac ggtgtcgata aatcactcat cacacctaat aataaaaaat aaaaaccata
    17641 tttttgcaaa aaaatcattt tcttccccta ctttttagtg attattcgat acatccaaca
    17701 atcaaatttt ttctcatttt aacacatatt tttattctag ttgtcttttg aacaatcaat
    17761 aacataaagg tgagtttctt ctacatttta aaaaatcgaa tggcactgaa gagttcagtt
    17821 gtcagcatca atctcctact caaaaaggca aaaaaacgta agtcagatgt tgttgacatc
    17881 tcacttacgc atccattgat tgaaaatttc ttagtaaaat ctactagcct aaaaatttga
    17941 agacttcttc tcgatcatac agtttaaata ctttctcgca taaaaattga gtaattagtg
    18001 ccgcaaaaac agggatgact aaccaacaaa gaacaacaag aaagccattt agccctgcat
    18061 ctaaagaagc caacggacca acaagtccga ccacgccgaa accagcagaa gcaggcgtac
    18121 ctgaaataga aaatagagcg acaggaatag ctgtaatcac tgcactaact aaacaaggaa
    18181 taagaatcac aggttttcta aatagattag gcatcatcat cttcatagcg cctaaagcaa
    18241 tcgcaattgt cacacctgac ttattaacct tccaagagtt cactactaaa acaaaagtgg
    18301 ttgcagctac gcccatcgca gccgcaccag cagaaacacc atctagttgg attgccattc
    18361 cgattgctac tgttgaaata ggagagataa tcaaaaaggc aaaggaacaa ccaatcaaga
    18421 tagacattaa cagcggttgt aaggtagtga agttattaat caatgatcca attgcagaag
    18481 taattgctgt tacaaaaggg aataaataaa aaccaagtaa acctgcacct acacctacaa
    18541 caatcggtgt taacacaatc gacactgacc caaaactatt tccaatcagt aaaatcagcc
    18601 cgacagcaat cgatgctgtc agcatcgtat taataatatc gcctgtacca gcaccaacgt
    18661 aaacacccgt ttcacccaaa tcaggtgtga atttgactac accagaacca acaaaagaag
    18721 ctccggctac aaccatcatt ctcatcggat caaatccaaa ttgtaaggca atcaaaccac
    18781 caatgatcaa aggtgttgct agttgaaaaa taactgctaa ctgtgcgatc gttaccgcaa
    18841 agggatacgc actaaaatat tttaaaatgg cacctaatac tgcattagga atcaatccaa
    18901 taatgattcc tgttgctgta ccagcgagga ctttattcag aaaaattttc ggtgatattt
    18961 tttgtgaagt ttccattcaa tctctccttt attttacgtt caaactatct tcttttgcat
    19021 ggacaagctc agtcaagaaa tcacaataag gcgtcgcaac accatatttc ttcccttttc
    19081 tagaaattgc gccattgata taatcgattt ctgttaatcg atggttctta atcaagtctt
    19141 gatacattga aggataatgt aacccaattg tttccgggtc aaaacaagat tcacaatgtg
    19201 caatgacttc tgggacatct agttcaatct tctctactgc tgctactttg gcaaactcat
    19261 tgacaatcgt cgccaccatt ttatgagcag tcgatgtttt tcctagctca gccatattaa
    19321 cgtctaagat tgtgcatagt ccgttcattg ttccgttcac acatgctttg cgataaatag
    19381 aatagtgaat attgtcagaa aaatgagcat ttaatcctga ttcagacaat ttgtctgcta
    19441 atttttttgc agctgcttct tttccatcac ctaaattttg taattctact gaaccacttc
    19501 caaataattt gacctgacca ggaccttcaa gacctgccgt ccacatggta tttccaatat
    19561 agatattttc cattggtaca aatttctcaa taatatcttc atgcccgata ccatttaata
    19621 gacataagac ctctgtatct tttttgatta atgattggat atcctgcagc attttttcca
    19681 gctgcatcgc tttggtaaat agaataatca gatcaacttg atcttctttt tctacttcgg
    19741 attgaagaac gattggtagt tttgcttcta cctctttacc attaaaattt gcttgtaatc
    19801 catgttcttt gatttgttgg acatgttctg cccagccatc gattaataat acttcgttcc
    19861 cactttggtg aagcatcaac ccaaagcgac ttcccattgc gccagcgcct gcaattgcta
    19921 ttttcattga ttcttcctcc tttttctttc tattatactc atttgagaat cttttcacaa
    19981 cctcgttttg ttattcttct cttatttatc atgtttatat ccaccattca agttatttta
    20041 tcagcaaatc aataaaaact ggaatagaat cccaatttca tcgtaagaga aactagacta
    20101 gcaataagaa aatagaaaca ttcatagaaa aaatctcact cctctgatta taaaagaggg
    20161 ggcattaacc tcgtgtatac taaaaccttc cattcattcg atggatgatt ttctttttgt
    20221 gaaaaaatat tcattgtagt acgatgttgc gcaacacaga cggatggtat tgcttattat
    20281 ttattaaaat cgaacaatac acttatttaa aaacttcttt cccagatttt cttccatttt
    20341 tacagttata actagtatta tcatgaaaaa tagcctttta ttgtctatgg gaataataat
    20401 ttaaaattgg tactttttta ttaat
//
LOCUS       DACDRK010000042        19958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDRK010000042 DACDRK010000000
VERSION     DACDRK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.042"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(51..914)
                     /locus_tag="IWJ77_001693"
     CDS             complement(51..914)
                     /locus_tag="IWJ77_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9453295.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1110..3209)
                     /locus_tag="IWJ77_001694"
     CDS             complement(1110..3209)
                     /locus_tag="IWJ77_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9453296.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3429..4538)
                     /gene="rpoD"
                     /locus_tag="IWJ77_001695"
     CDS             complement(3429..4538)
                     /gene="rpoD"
                     /locus_tag="IWJ77_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9453297.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(4562..6403)
                     /locus_tag="IWJ77_001696"
     CDS             complement(4562..6403)
                     /locus_tag="IWJ77_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9453298.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(6585..8978)
                     /locus_tag="IWJ77_001697"
     CDS             complement(6585..8978)
                     /locus_tag="IWJ77_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9453299.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9085..9945)
                     /locus_tag="IWJ77_001698"
     CDS             complement(9085..9945)
                     /locus_tag="IWJ77_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9453300.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10004..10525)
                     /locus_tag="IWJ77_001699"
     CDS             complement(10004..10525)
                     /locus_tag="IWJ77_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9453301.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            10845..11564
                     /locus_tag="IWJ77_001700"
     CDS             10845..11564
                     /locus_tag="IWJ77_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9453302.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            11590..14181
                     /gene="mprF"
                     /locus_tag="IWJ77_001701"
     CDS             11590..14181
                     /gene="mprF"
                     /locus_tag="IWJ77_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9453303.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14369..15556)
                     /gene="tuf"
                     /locus_tag="IWJ77_001702"
     CDS             complement(14369..15556)
                     /gene="tuf"
                     /locus_tag="IWJ77_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9453304.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            15924..16280
                     /locus_tag="IWJ77_001703"
     CDS             15924..16280
                     /locus_tag="IWJ77_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9453305.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(16521..17525)
                     /locus_tag="IWJ77_001704"
     CDS             complement(16521..17525)
                     /locus_tag="IWJ77_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9453306.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            17691..18542
                     /locus_tag="IWJ77_001705"
     CDS             17691..18542
                     /locus_tag="IWJ77_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9453307.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            18559..18804
                     /locus_tag="IWJ77_001706"
     CDS             18559..18804
                     /locus_tag="IWJ77_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453308.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            18991..19695
                     /locus_tag="IWJ77_001707"
     CDS             18991..19695
                     /locus_tag="IWJ77_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453309.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         5643 a         4084 c         3507 g         6724 t
ORIGIN      
        1 tgttaattaa aattattata agctagttaa gaaaaacatg caagggattc tcaattagat
       61 tttttgacta gttttgtcac accgttttct tgtgtaatca atccttgttt caaaagatta
      121 cctaatgcgc gcttaaatgc tcctttgctg ataccaaaag ctttcatgat ttcttctgga
      181 tccgatttat ctgtaaagtt gatttgctga tctgcagaac gttctaaaat ggttaaaatc
      241 atttgtgcat catctgatat agcttcatat gctcttggct tcaatgaaag attcagtaca
      301 ccgtctggtc ggacaccaat cactcgacct tttatctgct cgcctagacg aggttcatta
      361 tatcgttcag aaggatggat aaacccaaga tagaaatctt ccgttaaaac gtacgtacct
      421 gttaatttca agcggtatgc tgttcctatc acatctttgt ttttcatttc ttctgttcca
      481 tatttgctta aagatttgaa tattttttca tctgctaaac tgccccaaat acggtctttt
      541 gcatcaactc taagggaaat catcaagcga tccccttttt taggccatag ctctgtcatt
      601 gtcggcaatt catcaagaga tacgacaaag tctttatctg gaagaccgat atcgacaaac
      661 gcacctaaat cttttcgtgt agcagtaacg attccaaaag cgtaatgacc gatacggctt
      721 ttaggaattt gtgtagtgat ccgattttca tgtttttgat tttggtaacc gaatccttca
      781 actgcttcac cgattttgtg ttcaccttct gatttgctta agccaaaggt ttgtccgttt
      841 ttttggacaa agtagttttt ttcattttca tcaataataa tacctgtaaa tatagttgct
      901 aataaatcgt tcattttttt ctccctctat agtgaaaggt tttcttaata catttctttg
      961 ctatcataac ataatgttat ctacttgaat agctgagcct tggtttggaa aggtgaaaaa
     1021 aggagtatga caacagttgt gtcatactcc cctttttcga ataaacggtg ttaaacggca
     1081 ggctcacagc ctctttcatt tgcctaaaat taattgctcc catttcctcc gaaccatcca
     1141 gttttacagt tgatggttac gatgtaaaat ggcaaagcac tttctccaat gcctttcatc
     1201 aatgcagatt ttgttgaagt ggcatataga ttataatagc cttccccatt ttcgattctg
     1261 gctttttcta atctgtaacc tccttcaacg atatatccac gttggataca tgtagcaatc
     1321 actttgcttt ggacacctgg tgcccacgtc catgctggat tagatgcatc ggcaatatca
     1381 cttgctctgg cagatttgat tggctgattc gctgagtttg aagaggaagg ggtcgaggat
     1441 gccgcttctt gctgtgctgc ttgtgcgacc gcagcttgtt gagcggcttg agctgcagct
     1501 gctgcttgag cggcttgttt tgcttgttct tcagctgctt ttttcgcttc ttctgctttt
     1561 ttcttttcct cttcttcttt tttctcttta tcagtgagca tcttatctac ttgtttcaat
     1621 tgttcattca actgagcaga tagatctgta tttttcactt tttctactgc ttcttttgcc
     1681 gtcttgtatt ggtcgcgtgt tgctgaatcc actacttttc cgtctttaaa gactatagct
     1741 gttttttctt tagctgtttg caattcagca tattgatttt tagcttctgt ttgtgcttgt
     1801 tgaatcaatt ttccaaaatc tgtgttatcc gtttcgattg cttggatctc tttatctgct
     1861 ttcaacttga tgtcatctgc tagatgatca tcgacgatgg caggtgctat gaaccactca
     1921 ttcacttttt ggatatctcg gatcttagat tggatatctt ggtaagttgt ttctaactct
     1981 gaatatcctt tttcattctt aagactggat aaatcatttt ttagttttga taaatcttga
     2041 tgaatcatat ctgcacgaat aaattgatgg tgatcatctg tataaaaggc agcaagtttc
     2101 tcatgaagtt cttccatttg ttgctgtctt tttgcttctg ctgctacttc ggcttctact
     2161 ttttgatgat ggtcataata agcccagctt cctccaccga gtagaagtac tgctgctaaa
     2221 acgagataag gtactttttt tgattttttc ttttgcgctt tatctggatc tgttttttct
     2281 aagtttgatt cttgatgtgt tttttcagta tctggcacga ttgttattga tttcgcttct
     2341 gcttcaaaca gcttaggctg gttctcttct ttttctattt ttgcttcaag atcagtaccc
     2401 tctttgctta cagattgatt ttcagcatcg gattcactat ttatttcatc gggctctgtt
     2461 gactttttgt ctgtgttttc gagtatttct tttttctctg cttccggcag tttattcgtt
     2521 ttttcctttt ctaattgttt ttcagtaaat acttcagttt tttctgtgat atccgttttt
     2581 acgtctttta tttttactgt ttctttacct gtggcgtttt ctctatcaga gtctttttca
     2641 gtttcttgtt ccttttctgg ctctactttg ttttcctgtg caattttttc actgatcatt
     2701 ttgagcagct ctttcgcgtg ctcttctttt gtatcttcac gatgtgcttt gatataagcg
     2761 gatagtatcg aattttcttc ctcattatcg acgatttctt tcagttcagc tactcttctt
     2821 ttttgttttg attcaagcgt ttcttcttga ttctttcctg attcttccga agtctcatga
     2881 acagttgctt tcttctgttc atttttctct atattagtta cttgatcgtg agcaggtggt
     2941 tcttcgtctt ctggttctgc tttttcgaat aattccatta cagattctag aggtaaatct
     3001 ttcaattccg accattcgat tggatcgttt aattctgtat caggaaagac tgcatcttca
     3061 gttgatttat cctgcatagc tgcatctgta tttgtttctt caattgattc ttttatttgc
     3121 tgatcttctg cagttatttg atgaccgcag tctggacaga tagtattctg ctccattttt
     3181 ttcgatccgc aatttggaca ttttttcatc acttagcgat cggctccttt actaggttta
     3241 cgctaccatt taatctatca tattctatac gtcacgccaa tagcttattt ttaggattta
     3301 cttaaaaata atgaatgaat ttccttccta tagaaaaaaa agcttgagac tgcgtagtct
     3361 caagcttttt ttcttttaaa ataaaccaaa cagttttact gatttctctt ctggtcaata
     3421 gtccacgctt attctaagaa atcttttaat tgttttgaac gagaaggatg gcgcaatttt
     3481 cttaatgctt tagcctcgat ctgacgaata cgttcccgag taacaccaaa tactttccct
     3541 acttcttcca atgttctcgt ccggccatca tctaaaccaa aacgtaagcg aaggacattc
     3601 tcttcccgat cagtcaatgt atctagtaca tcttccaatt gttcttttag taactcataa
     3661 gctgcgtgtt ctgctggact tgttgcttcc tggtcttcta tgaaatcacc taagtgagag
     3721 tcatcttctt caccgattgg cgtttcaagg gaaacaggtt cttgcgcgat tttcaagatt
     3781 tctcggactt tttctgttgg aagatccatt tccgcaccga tttcttctgg cgtaggttct
     3841 cttccaaggt cttgcagcaa ttgacgttgg atacgtatca atttattaat tgtttcaacc
     3901 atatgaactg gaatacggat cgtacgtgct tggtcagcga ttgctcgagt gatggcttga
     3961 cgaatccacc atgtagcata ggttgaaaac ttaaatcctt tgcggtagtc aaatttttca
     4021 actgccttca tcagtcccat gtttccttct tggatcaagt caaggaactg catgcctcgt
     4081 cctacataac gtttagcgat acttactact aaacgaaggt ttgcttctgc cagacgctgt
     4141 ttcgcttctt ggtcgccttc ttcgattttc aatgctaacg ctacttcttc ttccgcagtt
     4201 aaaagggaga cacggccaat ttctttcaga tacatacgaa ccgggtcatt gatttttaca
     4261 ccggttggcg cagaaagatc ttctgttttt gcttgttcag cttttttctc agcactcttc
     4321 aagctgtgaa ttgatggatc accattttca tccaccacac tgatacctgc gtcttcaatc
     4381 ttttggatca atttgtccat ttcatctgca ttcaaagtga atggtgtagc aagctgattg
     4441 gataaatcat catatacaac ttgtcgtttg ggtttgtttt ctttgatgaa agctgctact
     4501 gctgcttcat acgttttttt atctgttcca tttgccataa aagaaggccc cccttgagta
     4561 gctatttggc ttgttttaac tgtttcgtta aattgatgat ttcaatcgtc aattcaagct
     4621 caagctgttt gtttccagtt cgtctggctt cttgctgcat ttgttttttt aatgttattt
     4681 catcagctaa gccagatttg ctgatcacat ctagcaagtc ttggatttct cgtggcgaac
     4741 tttcttctgc caccggtaaa tattcgattt ccaccaccaa ccgccttaaa ttttcatctt
     4801 gcaaaaagtc taaaaactct gcaaggttga attcataatg ttgtgagaca tatgcatcga
     4861 ataaaaaata aacttctgca tatgcatcat gaataaactg aaattctcgt tcttttaaaa
     4921 tattacggac agaaacttct ttaaatgcac gatataatag aatctgctct gccttttcta
     4981 cttgcgtcaa aggtctcttt ttccgtgcag tttcttgtct cgtaacctga gtgtgttgac
     5041 tttcctgagg ttcttgacga ttttgctgat ttgtccttcg aagatcgcgc agttgttttt
     5101 gtatcgtctc tctgccgatc tgaaactctg ttgacagttg agatagatac atatcttgtt
     5161 cgattggtga aacaactcgg ctaagttcaa ctagtaattc ttcgaggtat tcgatcttgt
     5221 ctttttcatt atctaaattt ttcccttgtt ttaaataacg tgatttaaaa gtaaagactg
     5281 tttcgcgccc atgagccact aattcttgga aagatgcttc tccatactta cggagatatt
     5341 catcaggatc aagcttttct ggaacgacta cgacagaaag tgctaatcga ctgttttgcc
     5401 gaagcaattc aatccctcga ttggttgctt caaaacctgc gttgtctccg tcatagcaaa
     5461 agaccaattc tttcgtcagc ttttccattt gttggatctg ttgagctgtt agacttgtcc
     5521 ccatagaagc taatccagtt ttgataccag cctgccatgc agcaatcaca tccataaacc
     5581 cttcgaacaa caaaatttct ccgtttttcc ggatatcgct tcttgcttta tcaagattaa
     5641 ataaaacttg tcttttattg aacagttctg tttctggact attcaagtat ttgggctgat
     5701 ctttttcctg ccctttttca ggtgcaagcc agcgtcctga aaaaccgatc gtctggcctt
     5761 gagaattacg aagtggaaac atcactcttt gataaaaacg atcagacata cggccatcat
     5821 catgcgtgac aaacaatcca gagcgttcta acaattcatt tgaaattgat tcattcttga
     5881 atacttgctg aagaaaagaa cgctcattag gtgcaaaacc tatgttgaat tcttcaatta
     5941 attctaaaga caacccacga ctttgcaaat attctaaagc tggctgtcca gcttttgtat
     6001 gcaatagcat gtgatgatag atttccgctg ctttttcatg agcagcaatc agtttccccg
     6061 tagcagattc aggattttgg acagctatag tacgctgacg ccattgatct tctaacggta
     6121 tctgttcgaa gtctgccact tttaccactg cttctggaaa acttagacct tctagttctt
     6181 gaagaaaagt aaacacgttt ccgcctttac cacaaccaaa gcaatgaaaa atctgcttgt
     6241 cttctgcaac agaaaaagac ggtgtacgtt cttcatgaaa aggacacagc cccaagtaat
     6301 ttttaccaga ctttttcaac tggacatatt gtccaatcac ctcaacgata ttcgtttgac
     6361 tgcgtactgt ttcgataacc tcttgaggaa tccgttgagc cattttttca ccccctgctc
     6421 tattgatatc ttttgggttt aacagaagat atttcaggag aataaccggt aaaaaatcgc
     6481 actaactgaa atcagtgcga ttttaaagga cacaattata catgtttgat tgtatcatat
     6541 ttcattgctt tttcaagtat aacacatttc tatctcatta agcatcaatt tgctactctt
     6601 ttcagtaact gattttgaat atcagccttc cagtacaata ctcgaagaac aatatttacg
     6661 agcatgaata caaagaagta tcctacgctc tctcccaacc ctaagtttac ttccaaaatt
     6721 ccatggaaca gcattgcagc tacatatatc cctacgatcg tcgttactgc taggaagatg
     6781 cgcaatgggt tgaagggata acaagctttt acaactgcca tgcaactgat cccaatcagc
     6841 aagtaataca tcaaagtagt agtttcagca tttccccatg cgaatgtttt tccaagtaga
     6901 tacaccgcaa tgatattcac tactacaagc aaggcatttg gtaaagcatt aataagtgct
     6961 gtaggcaaat aacggtaagt gatttttctt ttatcttgtt caaaagataa aaagaaggaa
     7021 ggataccctt caatcgctaa atcaatcagc gtgatttgga tagggataaa cggaaatcca
     7081 attgctgtaa cggcacatat aatcgataaa ataaatgaat aaatcgtttt gataaaaaag
     7141 atgcctgaga ctttcgtcac attgttgacc actcggcgtc cttcaaacag gacttctggc
     7201 aatgtagtaa aatcagagtc caacaatacc agattcgcaa tctgacgtgt tgctccatca
     7261 ccttctgcca tagcaatgct gcaatctgct tctcttaacg ccaaaacatc attcacaccg
     7321 tcgcctgtca ttgcaaccgt tctgccgctt tctttcaatt catttaccag caatttcttt
     7381 tgctgcggac tcactcgtcc aaaaaccgta tagcgatgag cagcttctcg cacttccatt
     7441 tcttcagtaa tttgagaaag atcgacatac gcatcataat tcggtaatcc tgcacgtcta
     7501 gcgatattgg agacagtaaa aggattgtct ccagaaatca ctttcagatc cacaccttcc
     7561 tcatgaagat aagctagtgt ttcttgagcg ttttttcgga ttggatcatc gatttcaaac
     7621 agagcaaccg gctcaacaaa tgctggtttt tcttcgttca attgctgatt tggagcgatc
     7681 cccaacataa gtacgcgata gccattcgct tgggctgttt ttagactgtc cggaagccga
     7741 tcttcttcca ccaaacgttc aggcgctcct acgtacacga ctcccagttc tggaaattcc
     7801 atcgcgcccc atttgcgatc agaagaaaat gcgactttgt ttgtcacatc ataccgatta
     7861 gaaagctcaa aataatctct catagcccgc atagtgatgt tgttatcact gctttctgcc
     7921 agatacgtcc ccataatttc tgggatggct ttctcgtaca gcggatgtag tgcttctacc
     7981 cgctggatac tcattttccc ttcagtaatg gtccctgttt tatccaagca taatgtatcg
     8041 acatgggcca gcgtttcaat tgaatacatg tcttgaacaa ggattcgttt ttttgccagt
     8101 ttggttaccc ctgtcgttaa tgcaatactt attagcaata ccaaaccctt tggaagcatg
     8161 cctaacaaag ctgctgcaga tgcaataaca gaaattttga tagaagcatc gcggatgaac
     8221 agtgcttcca aaaatagtat gatccccaaa ggaataatca cgaaactagt gaatttggat
     8281 actttgcgaa tcgaatctac aagttcagaa cgaatagctt tgtgagtctt tgcctcgcta
     8341 gtcaagcgga cagcataatt atctgcacct actcggataa cttcagccat gacagaaccg
     8401 cttgtcaaaa agcttccaga aagcaattca gctccagttt ctttgacaat caaatcagat
     8461 tcgcctgtca aaagagcttc atttgcttct gcccgtccag atataacctg taaatcggct
     8521 ggtatctgtt ctcctgctcc gatttttacc aaatctccta ataccagctc tttagtagaa
     8581 atcgattgtt ctttaccgtc ccggatcact agtacttctg attgggaaac aatcgataat
     8641 ttccgtacaa gatttcttgc atgtatttct tgataaattc caataacgat attgacgaca
     8701 ataattgcta aatacgccat gttggaatac gcacctacaa ataacagaca aatcccaata
     8761 ccaaagttca gaaaattgaa taacgtcaat atattatcgc tgaatatttg ttttgtactt
     8821 tttgatgcat cttcctggta gtcattttgc tggccagaag ccattttctg aactacctct
     8881 tgctgtgtaa gtccattcat tttctttttc tccattttcc gccaactttc aaaaataatc
     8941 acgtgtttaa gtctagcgtg ttccaagatt aaaaacaact tgtttttata aaatttatag
     9001 aaaaacacac gaatgatttt acaaaaaaga tgccaacctt tttgtttgaa tcacccgtgt
     9061 gctgttttta ttatcaatct atctttactt atattgttcc tccaaacggc tcatccacca
     9121 acctagtgct gtaccggcta taaaaagaac aggacaagct agtaccccag caactcccaa
     9181 tcccgcgtaa tcggtaggaa tgatcataat cgttgaagca aaaactacac ctaaaatgaa
     9241 atggaatagc ttgctatatg cctttgagaa aatgtaatcc atgatttttg acaatccaag
     9301 aacacaaaga atcccaccaa ttccgatcgg cacaatcact tcaagtcgta cttctttaaa
     9361 tccgtcagcc atcgctttat acaatcccat atacaccaaa aaattagaag ggctcaatcc
     9421 tgggacgatc atacccaaac caatcaatcc gccggcaatc agccatgttc caaaattctg
     9481 atcgacttgc ccgtcaaata attgagcgcc gtataataaa aacaaataag ccagaacaaa
     9541 agtaacaacc atgatgatca catcagaagt atctcttcct tttttccctg cttctttcca
     9601 taacgcagga acagttccag cgatacatcc gacaaaaaac cataatatcg ttgtttcata
     9661 cgcacctaat aaaaaactga ctaaaaaaga aagtagaaag atgcctgtca ctccgcctag
     9721 accaacaggc agaaaaaaca aaacattttc tttgaaattt tttgtaaggt gggctaaaaa
     9781 agaaatcagc cgctcataca tgccaaagac agctgctaat gcaccaccgc tgactcctgg
     9841 caagataaat cctgaaccga taaacattcc cttcacaaac cgtaacatcc agtctttggt
     9901 tgtgtacccc acttcttgat tttccatttt ttgccttttt tccactattt gcgcactcct
     9961 tctttcattt aattttattc atgattgaac atttcctgct attttactta aagacttttt
    10021 cataggtctc tattattttt tcattcattt tgacaatatc aggataaaac atgttacctc
    10081 cattatgata gagatagccg ccattgtaaa gcaaggactg gatccgccaa tatcggtatt
    10141 gtttcccttc ctcatttcct aaaagtgggc tcagcacatc tttagaatac gcttctgcta
    10201 attctactgt gttttctccg ccatgatttt ttacataggc aatataatct ttcccaaaat
    10261 tatatgcttg gacagctgtc cataaatcgc atccttgttt ctttgcttct actatcattt
    10321 ccgccaaata tttgacacct tgatcaatac tgtcagctgg attatcaatg atctcagctt
    10381 ctccatgcag actttccgag ctctgcatga gatctgtccc ttttcctttt gattcggtca
    10441 atattatcgc cttgatcaaa ttctcgtagt tttccatatg ataatttttt gcagctgttg
    10501 ttatttctat ttgatagctt tccactcgtt ttaccgaacg ataagttctt cctcataaaa
    10561 acaacaccgc accaataacc gtcagaatga tcatgtaaaa aagaatacgg ataatagata
    10621 atcgttttcg cttcatttca tcacctagtt attttgacat ctttttttat cttactattt
    10681 ctttatcttc ttggctattt cttccttgat ttctgtgtac gccttaagaa aaaatgaatt
    10741 ttcctgtatt ttttgacatc ttacgaaaat gatcgttgtt tttcagtaaa aacaattgcc
    10801 caatgactga tataatcgtt atactttaca cagtaggtga ttatgtgaaa aaagaaaaga
    10861 aatatgcaaa aatgccagat gggagcgaaa tttactatga aaagagcggt caaggcttcc
    10921 ctctcttttt actgcatggc aatgacggca gcggccgctt tttttcagaa caggtccctg
    10981 tactcgaacg ctactatacc gtgtatctag tagatagccg aggacatggt cgttcgacaa
    11041 acgaagcatc tatgttgaat tttcagttaa tggccgagga tctgaatacg atcatgttgc
    11101 tggaaaaaat cgatcaggca gattttcttg gatttagtga tggagcaaat ctagcactgg
    11161 tatttgccag cagcttccct aaaaaagttc atcgattgat tttaaattct ggaaacacgc
    11221 ttgttaaagg cgttcgtttt tctgccagag tcatcagtaa tattcattat gcatgggtat
    11281 ggttactctc tttatttcgc ccaagtcttc gcaaaaatct tttagttatc aaacttttgc
    11341 tgcatgacat cgggcttaca gaaaatgatt taaaaaaaat caactccccc actttgatca
    11401 tcgtgggaaa aaaagatgta atcaagctaa aacattcttt gtatatagct aaaactatac
    11461 caaaagcttc ctttgtgctt gtgaaagaac aaggacatga attagctaga aaagatcctg
    11521 aacgcttcaa tcgagaagtt cttcaatttc tttctgaaac ataactatta ggaatagaag
    11581 gtgatttatt tgttaaaaaa taccatacaa tggctgaaaa accacctagg actgttcaaa
    11641 acaatctttt tgatttctgt cattgtcatt attgtcggtg aattgatgtc catcggtaaa
    11701 actctatcta tccagcaatt aggagaaacg tttgccacta tacctgtatg gaaaagcgga
    11761 ctaatgctat tgattggtct tatctctgtg ttacctatgg ttggttatga tattattttg
    11821 aatcggatgc ttgaacaaaa gcagaatcct cgttacctgt ttgagacaag ctggctgatc
    11881 aatacgatca acaatattgc cggtttcggc gggtttgtta gtgtggggct tcgctctgaa
    11941 ctatatggac aaaagaaaga gagcagaaac gtgatccaag cattatcgaa agttttcttg
    12001 tttttgatgg ctggactttc tatttacagt ctgctttctt ttttacttgt agtcttcacg
    12061 ccagttgctc cttttttgaa gcaatattgg atatggttga tcggcggcgg tctatatttt
    12121 cctgtcgtct ttttatttac tactttcaaa aagaaaggat tccttggtgg tacctcactt
    12181 aaaactcaag gacagctatt gctagtttca ttgttggaat ggaccggtgt cctgtttagt
    12241 ttcctttctg ttggttattt aatggaaatc cggattgatc tgtggcaaac aattccatta
    12301 ttcattgctg cttcagtgat aggaatcgtg tctatgattc caggagaaat tgggagtttc
    12361 gatgtaatga tgattatcgg gttgtccgct attggtgtgc caagagaaat agtcgttgtg
    12421 tggatcttgc tctatcgcct gttctactac atcattccgt ttttgattgg aatcgtcttt
    12481 ttcttcaaaa atatcggttc cacttttgat cagcgttatt ctggtatccc taaacaatta
    12541 gcaacagaaa ttgcgcataa aatcgtggtt gtcttattat acttttctgg aatcatgcta
    12601 gtactttctg ccactatccc gcaagctttt acagagtttc gctggcttca tagtttgaac
    12661 ccattgaaat ttcattttat tatccaattt cctagtatac tattgggatt tcttttaatc
    12721 attatgggca gagggatcgc agcacgtgtg aaacgagcat atttgcctac aatcttcttg
    12781 attgttctag ctttacttta tgttttatta agtgatttta gtttcacacc ggtggttttc
    12841 ttgtctattc tgcttctgat cattcttgct tctaaaaacg aattatttcg ggaacaattg
    12901 atctattcat gggagtggcg tacgatcgat ggaatcatta ttggtgcact gtccttgctt
    12961 tacatcgtta ttggggtata caatctgccg gatttccctc atcggcgtca ccattttatt
    13021 tcctttttct tatttccttc tgaaaagatt tggttttcgg gattgttggc catcatcgct
    13081 gttagtttca tgatcgttct atttgttcat ttccttcaag gagaaaagaa acagattggc
    13141 gaagccttta atgaagaaaa ggccctgaag atccttacaa cttatggagg aaactccgat
    13201 agtcaattga tcttcttaaa agataagcgg atgtttgcct atgaaaaaga cggtgaacca
    13261 actgttcttc tccaattcgc ttgcttcaat aataaatgta ttgtaatggg tgatccttct
    13321 ggtaagaaag aagattttcc agaagcaatc gaagctttta tcgaagaaac agatcgacta
    13381 tgctatttac cagttttcta cgaaacaagc gaagagattg ttatgatttt gcatgaattc
    13441 ggctacgatt tcattaagat gggggaagaa gcttatgtag acctgaatag ttttacaact
    13501 tcagggaaaa aaatgaaggg cactcgtgct gtcttgaatc ggatcgaacg agaaggattt
    13561 acatttgatg tcttacaacc accattttct actgagcaaa tgagtacttt caaaaatatc
    13621 tctgataatt ggttgggttc acgaaaggaa aaagggtttt ctttaggctt tttctctgaa
    13681 gattatctac aaagagcgcc tattgcagtt gtcaaaaata cccaagaaga aattgtcgct
    13741 tttgcaacga tcatgcctac ttacacgaac aataaagtag gaacgattga tctgatgcga
    13801 tatgattcta aaaaagcacc ttctggcagt atggatttct tgttcttgca tttgtttgcc
    13861 tatatgaaag aagaacatat ccaatggttc aatttaggga tggcccctct ttcaaatgtc
    13921 ggaacttcca gaaagagttt tttacaggaa aggattgcat acttggttta cgagtttggt
    13981 tctcattttt attctttcca tggattaaga gaatataaaa gcaagtacgc tacaaactgg
    14041 gtctcacgtt atactctata ttctagagat agttggattg catatgttat gattgctttg
    14101 ctgattattg ataatgcgcc agttgagcag acacaaggta gaataagtgg tttgaaaaaa
    14161 tggatacgaa gaaagtatta aaaactgcat accgatagat gaacactctc atgatgaata
    14221 aaagagactg aagtaaaacg aaagttcatg cttctacaat ctcttcttat acaattgaag
    14281 aacaaagagc ggtccaaaca gaaatcctgt ttggaccgct cttttatttt aaacattttt
    14341 cattaccaaa atattcgagt cgcgtttctt atgcttcgat ctctgtaacg atacctgaac
    14401 ctactgtccg tccgccttca cgaattgaga atgtcgttcc gttttcaaca gcgattggat
    14461 ggatcaagtc aacatcaatc gtcacgttgt caccaggcat gaccatttct gtatcttctg
    14521 gcaatgtgat tgttcctgta acatcagttg tgcggaagta gaactgtgga cgatagttgt
    14581 tgaagaatgg agtatgacgt ccaccttctt cttttgtcaa cacgtacact tctgctttga
    14641 attttgtatg tggagtaatg gaacctggtt tagcaagtac ttgtccacgt tcgatatcgt
    14701 cacgttggat cccccgtaac aacacgccta cgttatcccc agcttccccg taatctaacg
    14761 ttttacggaa catttctaca cctgttacaa ctgctttttg tgtttcaggt ttgatcccta
    14821 cgatctctac ctcatcaccg acacgaacag caccacgatc aatacgacct gaggcaacag
    14881 ttcctcgacc tgtaattgag aagacgtctt ccactggcaa tagtaatggt ttatctgtat
    14941 cacgttctgg tgttgggata tattcatcta ctgtatccat caattccata atagcagctt
    15001 cagcatctgg atcgccttgc aatgctttta atgcggaacc tttgatcaca ggagtgtcat
    15061 cgcctgggaa accatattcg ctcaataact cgcgaacttc catttctacc aaatcgatca
    15121 attcttcatc atcgactaaa tcaactttgt tcaagaaaac aatcaggtat tttacaccaa
    15181 cttggcgtga caataaaatg tgttcacgtg tttgaggcat cggaccgtca gttgcagaaa
    15241 caactaagat tgcaccatcc atttgtgctg cccctgtaat catgtttttc acatagtccg
    15301 cgtgtcctgg tgcatcaata tgcgcgtagt ggcgtttttc tgtttcatat tctacgtgtg
    15361 cggtattgat cgtaatccca cgttcacgtt cttctggagc tgcatcaata ctagcatagt
    15421 cttgaggatt tgctaggcct tttttgccta atactgtagt gatcgcagca gttaaagtag
    15481 ttttcccatg gtcaacgtgt ccaatcgtac caatgttaac gtgtggttta cttctgtcat
    15541 aatgttcttt tgccataatt tacaaacctc ctaaataata tagatcactg aaaaatttca
    15601 gctctcacct ttttattata gtcttttttt gactaaatca aaaagaatac gtccgactta
    15661 ctttttgtaa gattcgaact aagcttatta tacgtcaata ttggtcaaag tcaactaaga
    15721 gcactcgtct gtcttatacg cgaaaaaagt accttttata aataaaaaaa tgtgtttttt
    15781 gagaaaaact tcaagatagt ccttaacttt cttttgaagc aataagaaca aaatgaggaa
    15841 aagtttaaaa ttcagctgtt gccaacaact ttcaatagac gaaatcttct atttcgttaa
    15901 taataaggaa agaggtgaag tacatgaaaa aattaattgg cgtacttgtc gttttaggca
    15961 tatttctcgg cggaagtttt gcagcttatc attattttta cggcggcgaa gcttattata
    16021 caaaaatcac gacgaatggc gaaatgtcag ctacccaagc agataacgga gagaaattca
    16081 cgacttatac ctacaagcaa gcggcttata ataaagatgg tgaaaaaaaa caggtgacgc
    16141 ttcgcgaaga aagagaacat ccattaaaga tgaatgcgta tcttaaactc aaagtcaatc
    16201 ctcgtaaagg tgtattaagc tgggaagaaa taaaggcttc tgaagtgcct aaaaatgcgg
    16261 cagaaaaaat cgataattag taaatggtct taaaatttta ttagataaaa agaggctggg
    16321 acagaagcgt ttaactccaa gaaataagaa ggagttgctt ctgcttccgc cgtttatacg
    16381 tgtttagagc gtgagacaaa agtgttcttt acttttgtct cacgctcttt ttttgttata
    16441 ttcgtctttt ccgatccttc tcttgacaca attcatttaa ctgatcactg gtacttgaac
    16501 acctaatatt ctagtcattt ttataaacga ttcaatactt catccacatt tttgatcatc
    16561 acggatatcg taccatccgg attattcaca aattcaatca aatctgggtt gcgatataca
    16621 tctactggta cgatcagttc gatcccgttt gatagcttga atttttgttt tccaaatttt
    16681 ttctcagaaa tttcttgtac ttcttttaca ggaggtgctt ctgggacaaa tccttgctct
    16741 tgtacttctt ctttaaaagc catttttgcc gtgatgtttt ccttgaagac tttttccgca
    16801 atcatctcat gatccaaccg acctttttct tctatcgtat catagacagc ttctttcaaa
    16861 tcagccatga tatcaaattc ttctgtctca aatttttttc caagatgctt ggctactttt
    16921 tggatcactt ttacattttc ttctaaagaa ggtgcaggaa cactttcgat cactcttcca
    16981 gaaaaataaa gctctttttt gccagaaaac tcatattttt tttcaattaa ttcgtaggtc
    17041 caatcagtca aattcacagc gaatgcttca tctgcttttt gtgttttcgc ccctagaatt
    17101 gctcgattca aaatcaagcg gttgtcgatc cctgcttcgt ctgcttcaac aaagtgagtg
    17161 taagcctcac gataattgac tttcaataaa gataagtaca tcactgtatc taattcatac
    17221 aagacaacaa atacatctgc actaggtgct tcttcacttt cttggtagat atcgaaccac
    17281 cgctgaacga ttgcttcact tgcttccaca aaattttctt gagcgatttt aagtgattca
    17341 acgatcatgc tttcttctgc tagttgtcca gtttttgttt gagcagaaga gattttttgg
    17401 atcttctttt gaagaaaatc acgaatatat tcttttgtca gatccagctc tttttgtgaa
    17461 aagactggag aaccagtttc tctgtcaata atatgcagaa ttgctttttt taaatagata
    17521 tccattttct cattcccttc tgtcttagtt tacggaaaaa aaatcaaaaa taccagaatc
    17581 aaaatcattt tcttttaaac aaaaatttca agagtagaaa caaaatggat tctttttttc
    17641 gaaaaatgcg ctacacttat agttgaacga ataaaaaagg agctgaaagt atgaattcat
    17701 taacaagcac ttatcgtcta tcaaatggtt acgaaatccc ggtagtagga ttcggcacat
    17761 ggcaaacacc tgatggggat gtcgctgttt cctctgttaa agaagcgctg gctgcaggtt
    17821 atcgccatat cgatactgcc caaggataca agaatgaaga aagcgtcggc caagcgatta
    17881 aggaaagtgg aattcctaga gaagagatat tcttgacaac aaaattatgg aatgcaaacc
    17941 acagctatga acttgtgatg tcttcttttg aagaatcatt gaagaagctc caaacagatt
    18001 atttagatct gtttttgatc cactggccaa accctgttgc ttttcgtgat aattgggaac
    18061 aagcaaatgc agatacatgg cgagcatttg aagaactgta tgaagcaggt aaaatcaaag
    18121 caatcggtgt gagcaatttc ttaccccacc atctagatac actagcaaaa acagcaaaaa
    18181 ttatgccgat ggtcaatcaa gttttccttg caccaggcga attacagaca actgtagttg
    18241 agtatgcaaa aaaacatgat atgatcctcg aagcatatag tccattaggc actggtaaaa
    18301 tcttcgatgt gcctgaaatg aaacaaatcg ctgaagcaca tgacaagacg attgcacaag
    18361 ttgctttgcg ttggtcgctg caacacgaat tccttcctct tcctaagtca gtgacaccaa
    18421 gcagaatcaa agaaaatact gaattatttg attttgaatt aacagaagaa gaaatgaaac
    18481 aaatcgatca gttagatggt gtagtcggca aagcaaaaga cccagatact acccaatttt
    18541 aagtcgaaag gtgattttat ggatccaatc gaacgcttga attctctctc ggaagaagtt
    18601 acccagactt ttcattccga ttttgtcttt ttgatcgatg cagagaaaat ccaacatttt
    18661 cctgcaagaa actggacaca tgatcaaatc atcgaagaac tgaaaaaacg gttcgatcat
    18721 tcgctaatgg tcaaaccctg gcacgaacat gaagtcattt attcacctga actacctgtt
    18781 tttgccttga taccgaaaaa atagaaagaa tggaacggat tatgtatata tacataatcc
    18841 gttccatttt ttatttttct ttcaaaaaac gggaaaatac gcaaattttt ctgttgtaat
    18901 ttgcgtatac gcaagttttt ttgcattact ccttttcatt tggctgtttt tttcttataa
    18961 tcgaaagggt atgagcgaag gagagaacat atgaagaaaa aacagaaaca aaaattatta
    19021 aatcaattcc gtccttcatt ggatggtgta agaggacaat tattccgttc tctagaagaa
    19081 gagagcttgc gacgttatgg tttgccgtta caagtcatgc ttgatcccaa aaaccgtcaa
    19141 atcttaacga tcggattagc tgggaaaaca aatgaagata cacggatcaa cgtgccaatc
    19201 gatgacaact tcaatactgt attaaagaga attcgttcag gagaaaacgg gattttcgaa
    19261 cgattccgtg acaatcttct tattgaaatc gtgtcttact ggaatgatca acgattgaaa
    19321 aacgatgaac caactgcgca agctgttgcg acaccagtta cagaagaaac agtcagtgtg
    19381 ccagaagaaa aaacggaaaa accaaaagcg gacacaactg aaaatacacg ctcaactgat
    19441 acgaatgccg ctttaagttt tgctgatttt tcaaaagccg ttgctgagta tcctaaattt
    19501 tacacagaac aaaatgaaca tgaagtagtt attaatgaaa aaaatgctga tgaacctcgt
    19561 aaattagcaa ctatttctat gactactgaa aatgagttcg ttatcgaaaa agcactcgaa
    19621 cggaaataca aagtcaaatt gactttgatt cctttgatcg aacaatttgc agcgacagcg
    19681 attactgatc gataaaaatt ctatttgtca taaaaggctg ggatttaaaa tcccagcctt
    19741 tttagtcata taataatttc tacagcaagt aaaaagaaat tccttctaac atcaggctca
    19801 aatatcagct tgacctgtcc aacaaatcac ctatgatcct acaatgttag gcgacttctc
    19861 ttctacctga agatatcttg gagttgcctg atttcttaag atgcctaacc ttaactataa
    19921 aaaattctaa taataaacat attttctgat agagaaaa
//
LOCUS       DACDRK010000043        19611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDRK010000043 DACDRK010000000
VERSION     DACDRK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.043"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IWJ77_001708"
     CDS             <1..152
                     /locus_tag="IWJ77_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453310.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IWJ77_001709"
     CDS             complement(230..763)
                     /locus_tag="IWJ77_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9453311.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWJ77_001710"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWJ77_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9453312.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWJ77_001711"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWJ77_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9453313.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IWJ77_001712"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IWJ77_001712"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9453314.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IWJ77_001713"
     CDS             complement(4488..5066)
                     /locus_tag="IWJ77_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9453315.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="IWJ77_001714"
     CDS             5388..5858
                     /locus_tag="IWJ77_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9453316.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="IWJ77_001715"
     CDS             complement(5966..7192)
                     /locus_tag="IWJ77_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9453317.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="IWJ77_001716"
     CDS             complement(7946..8146)
                     /locus_tag="IWJ77_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9453318.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="IWJ77_001717"
     CDS             complement(8280..8888)
                     /locus_tag="IWJ77_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9453319.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="IWJ77_001718"
     CDS             complement(8973..9443)
                     /locus_tag="IWJ77_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9453320.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="IWJ77_001719"
     CDS             complement(9491..10405)
                     /locus_tag="IWJ77_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9453321.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="IWJ77_001720"
     CDS             10551..11354
                     /locus_tag="IWJ77_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9453322.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="IWJ77_001721"
     CDS             11532..12479
                     /locus_tag="IWJ77_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9453323.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="IWJ77_001722"
     CDS             complement(12547..12840)
                     /locus_tag="IWJ77_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9453324.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="IWJ77_001723"
     CDS             complement(12868..13533)
                     /locus_tag="IWJ77_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9453325.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="IWJ77_001724"
     CDS             complement(13673..14305)
                     /locus_tag="IWJ77_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9453326.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="IWJ77_001725"
     CDS             complement(14312..15379)
                     /locus_tag="IWJ77_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9453327.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="IWJ77_001726"
     CDS             complement(15376..16107)
                     /locus_tag="IWJ77_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9453328.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWJ77_001727"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWJ77_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9453329.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWJ77_001728"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWJ77_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9453330.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="IWJ77_001729"
     CDS             18270..19439
                     /locus_tag="IWJ77_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9453331.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         5914 a         3943 c         3140 g         6614 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa a
//
LOCUS       DACDRK010000044        19473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDRK010000044 DACDRK010000000
VERSION     DACDRK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.044"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(19..993)
                     /locus_tag="IWJ77_001730"
     CDS             complement(19..993)
                     /locus_tag="IWJ77_001730"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9453332.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1073..1948)
                     /locus_tag="IWJ77_001731"
     CDS             complement(1073..1948)
                     /locus_tag="IWJ77_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9453333.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2078..3985
                     /locus_tag="IWJ77_001732"
     CDS             2078..3985
                     /locus_tag="IWJ77_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9453334.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4526..7804)
                     /locus_tag="IWJ77_001733"
     CDS             complement(4526..7804)
                     /locus_tag="IWJ77_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9453335.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8075..8620)
                     /locus_tag="IWJ77_001734"
     CDS             complement(8075..8620)
                     /locus_tag="IWJ77_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9453336.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8632..9324)
                     /locus_tag="IWJ77_001735"
     CDS             complement(8632..9324)
                     /locus_tag="IWJ77_001735"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9453337.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9343..9627)
                     /locus_tag="IWJ77_001736"
     CDS             complement(9343..9627)
                     /locus_tag="IWJ77_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453338.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9640..10203)
                     /locus_tag="IWJ77_001737"
     CDS             complement(9640..10203)
                     /locus_tag="IWJ77_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9453339.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10431..11093
                     /locus_tag="IWJ77_001738"
     CDS             10431..11093
                     /locus_tag="IWJ77_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9453340.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11217..12413
                     /locus_tag="IWJ77_001739"
     CDS             11217..12413
                     /locus_tag="IWJ77_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9453341.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12514..13890)
                     /locus_tag="IWJ77_001740"
     CDS             complement(12514..13890)
                     /locus_tag="IWJ77_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9453342.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13927..14793)
                     /locus_tag="IWJ77_001741"
                     /pseudo
     CDS             complement(13927..14793)
                     /locus_tag="IWJ77_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(14882..15907)
                     /locus_tag="IWJ77_001742"
     CDS             complement(14882..15907)
                     /locus_tag="IWJ77_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9453343.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15925..16599)
                     /locus_tag="IWJ77_001743"
     CDS             complement(15925..16599)
                     /locus_tag="IWJ77_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9453344.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16783..17676)
                     /locus_tag="IWJ77_001744"
     CDS             complement(16783..17676)
                     /locus_tag="IWJ77_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9453345.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17750..19447)
                     /locus_tag="IWJ77_001745"
     CDS             complement(17750..19447)
                     /locus_tag="IWJ77_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9453346.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3444 g         6156 t
ORIGIN      
        1 tttccctctt ttttttgttt attcgaattc tttttcttcc agacggaata acggactcat
       61 cggtgtattt tcatggatgc gtttgaccgc ttcacccatt aattctgaac atgtaacgat
      121 tgttaagttt tctggatgac gattttcttc tgtgaaaaca gaatcagtga tacaaatatc
      181 tttgattggt gcattgtcta aaatactttt cgcatgatcg gacaataatc catgtgacgc
      241 acaggcatat acttcttttg cgccgttttc ttttaataca tttgctgctc gtgcaagcgt
      301 tgctcctgtg ttcaagatat catctaccat gatacatgtc tttccttgaa catcaccgat
      361 cacataacca ccctcagttg aagcatcatc tgcatgatct acgatggcta atgtgctgtt
      421 caaatattct gaaagactac gtgcacgctg aacaccgctg ttttttggcg aaacaactac
      481 gatgtcatct cccgtcagac ctaattgacg gtaataatga gcaaacaacg gcatcgtaaa
      541 taaattatct acaggaatat caaagaatcc ttgtacttga accgtgtgca gatctaaagt
      601 cagtactcga gttgcccctg cttcttcgat caagttagcg atcaatttcg cagtgatcgg
      661 ttcatgaggt ttcgccgtgc ggtcttgcct agcataccca tagtaaggca atacgacatt
      721 gatcgttttc gcacttgctc gcttcattgc atcgatcatg atcagtagtt ccatatagta
      781 gtcattgaca ggtgcattcg ttgattgaac gatatacaca tgatcccctc gaatactttc
      841 ttcgatattg atggaaatct cgccatcact aaattgcttc acgacgcttt tacctaattc
      901 tgttccgaac actttcgcga ttttttcagc taatggacgg ttgccattca aactaaaaat
      961 cttcaacgtg tcatcctgat acttattact catgtagtcc tccccaaaac ttctctattc
     1021 ttcgatagtt taacatattt ttccaagatt ttctgttaat ttcagcaaat tcttaaggaa
     1081 gtgaaactgt tccgttactc caactggcag gaatgacttt atgataatcg ccccagttgt
     1141 cgtgtacttt gtaataacgt tctcctgttt cagtttcaaa ataagcgtac gctgtcaccc
     1201 aatgattgcc atatgtactg ccaagtactt tcaaaatacc aatcatcaca ggtttgcctt
     1261 cacgtatacg ttttgtagct cgctgccatg aacctagaag agttgctcgt gcacgctcat
     1321 gattgccatt cttctttaaa aaactagaaa tccctgtact aacctgaaaa ggaatcgtag
     1381 gaagtcctag tggttggatc tggctacgca atgccttcgt caaaaccaac gaatcagccg
     1441 tatttttctt gcgaatctca tttggaagca tccacccctt acgatagtct tgataatagg
     1501 caaggagaac tgctgccgcg taagtaccac aaagatagcc tgagggggta gcccatttct
     1561 tatacctcaa aaaacgttct tctggcaatc ccacccagca atttgcgagt tcctttcctg
     1621 accttttttc ttcgatcaaa acgggaatat cttcttttct gttgatccat ctagataact
     1681 cgattggctc ttcattgatc agcttccatt ttttacaaga aagaccctct tgataagtat
     1741 attttgctgg agaatattct accggatgtc ctgattgatc gataatcaca taactttgat
     1801 cttcaaaaac cagagctaaa aaaagcaatt tactttttct tgactgcagc tgctgagtgc
     1861 tggctaattt tttaattttt ttatctactt tgaaatataa aggtttatcc actacttgat
     1921 tcagttggga tcgcctcctt tttttcatta taagtgaagg attgaaaaaa acaaatcagt
     1981 ttgattgttc gaattttcaa ttttttatgt tctatttgtt aattccttca ttttgtaaga
     2041 taatgggcac acgaaacaaa aaaggagcga tcgatgaatg aacaaagaac gattgaaaca
     2101 agatttctat gaagcagtca atgaagaatg gcaaaaaaca gcaaaaatcc ctgcagataa
     2161 accagctaca ggcggtttcc aagatttagt tgacgggatc gaccaacttt tgatgggaga
     2221 aacagacgat ctgcttgccc atccagaaaa agtgtcttca gaaaaaatga aacatttcct
     2281 tgcatattat caactggcaa acgattatga ccgaagaaat gagttgcatg ctcaaccgct
     2341 tcaacctata atcgaaaaag ttgaatcatt gcagtctttc gacgaattga ataagcaatt
     2401 tccagaatgg ctgttggata cattaccatt gccatttaat ctggatgtag atgcagatat
     2461 gaagaatgcg caaacgaacg ccttctttgc ttatccgcct tctttattcc ttccagataa
     2521 gacgtattat gcatcagacc atcccaacgg tgcacaacta ataggcgttt tctttgatat
     2581 ggctgttcag ttattagagc ttgccggcaa agaaaaaagt caggcagaag caatcacgga
     2641 acaagcagtg caatttgata aattgattgc tcctcatgta aaaagtgcag aagaaaacgc
     2701 agattacagc aaaatgtata atccgcaatc ttttgataca tttattaaat acacagataa
     2761 actggacttg aaaaaacttg tcacgggttt gattgggaca actccagata aagtgatcgt
     2821 aactgatcca gtgtacttcg atcatttgaa tgacatcctg acagaagctc attttccgtt
     2881 attcaaaagc tggatgatca ttaaaacagt acgttctctt agtggttatc tatcagaaga
     2941 attccgccaa gtcagcggaa tctattctcg cgtattgtct ggaacagacg aagcaatgcc
     3001 tcagaaaaaa gcagcgtatt accttgcctc tggacaattc gatcaagtag ttggcgatta
     3061 ctacggaaaa aaatattttg gcgaagctgc taaaaaagac gtagaacaaa tggtgaaaaa
     3121 aatgatcgcc gtttataaaa aacgtctaga agaaaatgac tggcttgggg atgcaacaaa
     3181 agaaaaagcc gtcataaaat taaataactt aggtatccaa gtaggctacc ccgataagat
     3241 cccagcatta ttcgaccagt tcaaaacagt acctgcagat cagggtggta ctttgctttc
     3301 aaatactctt gttttcagca aactcacatt aaaagatcgt ttcagcaaat ggaacaaacc
     3361 agtggatcgt tcagaatggg aaatgagtgc tgacacggtc aatgcttatt atcatccatt
     3421 tcggaatgtg atcgtctttc cggcagcgat ccttcaagca ccattttaca gtctagaaca
     3481 atcaagctct gctaattatg gtggaatcgg agctgttatc gctcatgaga tttcccatgc
     3541 atttgataat aacggcgctt tatttgacga atatggcaac ttgaacaact ggtggacaga
     3601 agaagatctt acacaatttc aaaacaaagc acaagcaatg atcgaagaat tcgatggtct
     3661 gccttttgct gacggaaaag taaacggaaa attaaccgtt tcggaaaata ttgctgatgc
     3721 aggcggtcta agctgtgcat tggaagcagc aaaaacggaa tcagatcttt ctatcgagga
     3781 attctttgtc aattgggcaa cgatttggcg tacaaaagca aaaaaagaat accaacaact
     3841 tttactgcaa atcgatgtcc atgcccctgc taaactccga gcaaacgtgc aaccaaaaaa
     3901 ccttgacgaa ttctatgaaa catttggtat cacggaacaa gacgctatgt atctagcgcc
     3961 agaaaaaaga gtgcgaatct ggtaagaggt tgtgccagaa gtggtcagct tcaagaaata
     4021 agaaggaatt tgggaaaatc gcttctcaaa tttttgtgac atttcggctt atttttcgaa
     4081 gaagttactt ctggaatacc atgtattaga ggttgtgaaa aagctgctta gtttcaagta
     4141 gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga cttactaccg
     4201 agaaactggc ttttgaacac cgtttatcga gaggttgtga aatcagcgtc ctgacctgag
     4261 tattagagaa aaaaacggaa aaatagttcc tcatattttg ccgtttgttg agctaatacc
     4321 gagggtcagc tgattgaaca ccgtttagtg gagagattgt gaaaaagctg tcctgcatca
     4381 agtaataaga acagccaatc aaaagcagca aggagaacct catctataaa aagatagaag
     4441 gaagtgagac atctattggt ctcatttcct tctattttta ctttcctcag tcaattgttt
     4501 tacatcataa aaggagaaat tccacttatt tttttcgctg tttcatgaca taaacaacta
     4561 caccgattaa caaaattcct ccagctaatg ctagcaacgg gttattaaat tctcccgttt
     4621 ttgggagtcc ttgagtgcca tttttttcac tttcttttgc tgtcctatcc ggatgagaag
     4681 gttttccatt tggttttcca tttggtgatt catttcctgg attttctctt tctggtttgt
     4741 catttcccgg gggcacatcg gcagtttctc gcacagtgat cgctttttgc tgggtaacag
     4801 attctgtatg agcagaccat ttttccccat caaatatttc cgaacgataa gtgacttcta
     4861 gcgtatattc tccttttgaa ggtgctgaaa aaatagcggt atgattagaa aattgagcac
     4921 ttgactgtgt ttcaacgatt tggatctttt ctgggaaata acgcatatct tcaaagacag
     4981 gcgttccttg atttggtgtg aaagttgaca caaaaactgt aaaaggcttc agttcaatga
     5041 tatttccttc tgcattctcc gccacaccat ttgcttcttg tataataatc ggattatttt
     5101 gttcatttgg ttttgtctga tttggttctt gtacgattgc atactctgga tattccgaag
     5161 acggtgaggt ttctccgtca aaaatagcac ctacagataa tggaagacca tccactcttt
     5221 ccttttctgt ttgacgattc cccggtttaa tctgcatcag tccatgaaca tggatacttc
     5281 cattttcata gcgcgtaaca ggctgctgac cttctttcga acatgtattt agagactcga
     5341 tccaatcttt tgatatggta tgtccaagag tattttctga ttgtttctct cgttcattcc
     5401 aatagtagtt caacgagtgg tagatttgat cttcgttttg attcaatggg cggatctgct
     5461 ctctcatctc aagattctct gttcggaaag ataagatacc agaagcatgg tagtctgtgt
     5521 ttcctgctga agaggtataa agagccacat tatatgaacc gttattaaaa cttgtactgc
     5581 tattgacgat aatatctgtt ccgctattgg aatcgattcc tttcgcaaga ttttcgaaag
     5641 acaaactatt gatcaattca tgagggcccg ttaaagaaga accgcccatt ttgaatccat
     5701 taccgttacc ttctccctca attcctggta cccagccgtt tcgataagca actgagtttt
     5761 tgatgattac ttttccgatc gaacctgttt catttttagc aaaaaggtcc caaccgtcat
     5821 ctgcattatg atacgcgata caaccgtcaa atacatttcc ttctcctatt gtcagtttgg
     5881 ccgcaaatcc atcggcatct tcaaatcctg gatcggcatt ttcaaaagat gtacagttct
     5941 tgactaagtt atgcgcgggc catgcttcga aaggatcatt tgagctaccg ctgatttgga
     6001 ttcctgtatt tccattccga taagcatgaa tctcttcgat caaattatga tttccagaaa
     6061 tctgtaatcc tttttgcata gctgcactat ttgtaatgtc aaaccctcgt aaaccccagt
     6121 agtgactgaa cagtgtcatc ccatttccag ttcctttaaa atcaagtaca ggacgcttgt
     6181 tttctggatt tttctctgca atcaactgaa tcgggttttc tcttgtcccg ttaacgcctt
     6241 ttggaatcgt caatcctctt tcgaaggaat aagttccagg cgccatgacg attgtctgcc
     6301 cagcatatgc gtatctcaat gcttgtgtca gagataatgg attttgtctg cttccgtttc
     6361 ctgcatctga cccttctggt gttacataaa ctgtcgaata tttaggttta cggtagtcca
     6421 ctgtatgaag aatctctttt gtttcataac tagtcatctc tacgtattct tctggcggat
     6481 agtttttatc tggcgtaaaa ctgaaagtaa attcgttcgt tccttccatc agttttgtct
     6541 gataatcaaa ttcccgattc gcagtgatag ccaagttttt tgcgttctta agcacctctc
     6601 ctgttgcttt atttttaatt gtcaataatc cgtcagcatt tgctctaaag gacatagtat
     6661 gatcttggtt tcctgaatga ttactagagg tcacagtgta aatcggatca atcaagcgag
     6721 taggtctttc ttgcccagga ggatctgtcg caggatctga gtaagcaact tcgattcctt
     6781 ctaccttgat cgtcatattc cttgctgcag caaatcctgc ataaataacc gattcatctg
     6841 aagcgtataa tttttcccag tcatacatga tgtcagaagc catttcttct cctgtttcag
     6901 gatcgtaata atatgactct aaaccagtat tcgttttttt catctctacg tccagtttag
     6961 taatcgagtt cgtggccgga aacaaattcc catgaccgtt tccgaatata ttataagaac
     7021 ctgtttcttt ttccaagaac cctgctgaag tctctaaagg tgttgtttcc actgagacac
     7081 tcccaggagc tggcgcagct tgcggatctt cagatgatat tcctgtgatt gcccgtgtcc
     7141 ccaatccaag tcgcatcgta tatttgctgc cgctcgtgtt gaccacttct tgtctatccg
     7201 agtcccagac atattcgatc cgactgccta gtatggcaaa tgaatttgaa taaaatgcct
     7261 gcccaaacaa atgatttttt ggtactgcgt ctcttaccat taaagcaaat ccttcttggg
     7321 cattcgtata ggtccaagat tctatttcga tcgtcgcccg catcctaaag ttgatgtctt
     7381 tggggattgc ttgataataa taggtcattc catctgatcc gcttgactgg aattttccgc
     7441 cgttattcgt ggaagagagc cgcacgccgt cttctaatgg ttcaatggca tacctactag
     7501 cacttgtgct ttctccgaaa acaacccctt tccactcttc atttgcttct tcagattgct
     7561 tttttgtctt ttcattttct gattctttca tcataggatc atttccttct tccgtatcca
     7621 tatcttcact agttcttggt acttcctcca ttgttgacat cccttcaatg ttcacgtgtg
     7681 cattttcttc gttcacagac tgttgttcgt tagcagcaac ttcaacgaca gaaattaaaa
     7741 ccggggtact gagcataagg agattcatca ttagcgcact gtattttata cgttttttta
     7801 acaagtgtat tcctcccgta acaaattaga aagcgtttac ttaaagtatt acctaattta
     7861 tttttactgt caataaaaaa caaaattata ataagtgtat tttataaaaa gaaaaaatat
     7921 aacttaagtt gttgataata aagagattta ttaacgtaaa aagaataagt ttttttaaaa
     7981 taacgtataa aaaagagcat aaaacaaagt cgtttgtttt atgctcttct tttattattt
     8041 tgtgcttttt attttatcaa atcactttgt ttttttataa aatttctgcg cctagtgtgg
     8101 actcaaaatg tctcaatgcc cattcatgtc caacgggatc gaaacttgcc acggcatctt
     8161 tgaaaatatg gagtttgtat cctagattat acgcatcgac tgctgtatgc aacacacaga
     8221 tatctgtaca tacgccggtc aaataaatat ctgtgatctg gcgttctcgc aaacggatgt
     8281 ctagatctgt tccactaaaa gcggaataat gccgtttgtc tatccaataa acattttcct
     8341 cttgctcata gtcttggtat aaacttttta aagagccgta caattcccgt ccacccgttc
     8401 ctatcacatt atgcggagga aataagcggt tctccggatg aaaggaatct tttggatcat
     8461 gcgcatcaat ggcaaaaacg acaaaatcct tttgatcaat aaacttcttt gtatgagcag
     8521 tcagcttttt ttcaattact tgtcctgctg tcccagtagt cagctttcca tcctctgcaa
     8581 caaaatcata tgtgtagtca atcgagatca atgcgttcat cttagtcctc cttatacgag
     8641 atgtttgaca aattcgatca ccatttctgc tgatcgtttg cctgcgtctt cgataaattc
     8701 gtcaaagctt tgtgtcgccg agtgatctgc tgtgtcgctc attgcacgta cgatcaaaaa
     8761 tgggatattg aactgacgag ctgtttgtcc aacagcggct ccttccattt cgcaagctaa
     8821 tgcttcaggg aaattcgtta agatttcttt gattttatct ggcgagtcga caaatgtatc
     8881 acctgtaacg atcaatcctt cttttgcgtt caaattcgtt gcttttgctg ctttaaccat
     8941 ttcactgcgc aaatatgtgc ttgcttcata atatagaggc attcctggta attgtcctgg
     9001 tttgtagcca aatcctgttg catctgcatc aaaataagct actttgtctg atataacgat
     9061 gtcacctact tgtaagcctt caccgattcc gccagctgaa cctgtattga tcaccatgtt
     9121 cacgccgtat tgttggatca acagacttgt ggtaatagat gctaagactt ttcctatacc
     9181 ggaacgtacg acgatcactt cgtggttacc taatgaacct gaaataaata aagcgcctgc
     9241 gcgttcccat gataatggtt cgcttaggtt ctctctcaaa atttttactt cttcttccat
     9301 tgcgccgata ataccgattt tcatcttttt aattccttct ttctatataa atgcgattgc
     9361 taaaaaaacc acacataaca ttaggataac aataataaga gatttcatca aaaaagagtt
     9421 ccatttggtc gtcttttccc gttcgctgat ccttgttttc gtgattggtt tattcttttt
     9481 gctttcttta cgataaaagc tggcaatctt tttttcttct tgctgataag ctgtttctgc
     9541 ttttcgctgt ttttttaatg actcgcttgc ttgtgcttgt tgtcgcttgc gaagttcgct
     9601 tcgcgtaata agcggtcctt tggccataag ccgatccctc tactttcctt tggtcaatag
     9661 ttcccagtat tgaatcgcat aaatcgtctt tgcatcacaa atcaactgat ctttcatcgc
     9721 ttgttgcgcc tcttcaagag ttagatgata caactccagt acttcgtctt catcttgtgc
     9781 taaaggattc tcgaccttct ctaccccttg tgcatgataa atatgaagga cttcatttgc
     9841 aaaacctggt gaaagataca tcgaagtaag atggctcagc gacttcgcac gatagccagt
     9901 ttcttcttct aattctctag ctgctgtcat ttcgggattt tgtccttctc ctggatcgat
     9961 ttttccagct ggaatctcca aaatcacttt ttctaaaggt tttcgaaatt gtttgacaag
    10021 aagcagacga tcttgttcgt caaaagcaat gatcccgaca ccaccaggat gaaataccag
    10081 ttctctcttc gaagttcctc catctggcaa acgaacttcg tctacagcaa catcaataat
    10141 ttttccgcta tagatttctt tgcgactgat tgttttttct tcaaattgct cattgttcag
    10201 catatcttct cctgctttct ttgtaatcat aactgctttt ttttcatttt tcaatttttc
    10261 tcaaaaactc cttattagtt taccatagat aaaccttaga ttttctctaa atatagaaaa
    10321 tccttcttta gtccgatact ctttctagta gtccgtatgc taaaataagt atgtaatcga
    10381 ataacgaaaa gaattcaata ctcaacgata aatgacatgg agggtttatt atgataaaac
    10441 gaaattggcc gtggcttcta gcattggtct tgctatttct ctttgtccga aacaatagtt
    10501 tttcttttgt tctaggactt ttattgatag gtgtagtttt agttttgtgg ggactctttg
    10561 gttcgcgcag gaaaaaggga aaacaagaag aaattcctgc actgactgac gaacttgaaa
    10621 ctcactatga aaaaagcggt atgacagcga atgaaatttc attttttcga caaacaatga
    10681 atcaaacaaa aaatgagatc aatcaattgc aaatgaatat gcagcagaca gccaaactta
    10741 aagcaatcga tctccgccat gatccagtca aagcagcgaa agctttattc aaagaattgg
    10801 tgaaagaacc aaaccgactg catgaagcaa gtcagtttct ttatacgcat ctgccgaatc
    10861 tagttgattt gacaaataag tataatgaga tcaatgacca tgagatcaaa aacaaacaga
    10921 cctatgaaaa actagaagaa agcgcgcaga tcatcgacca gctgtcagct ttaatcgcac
    10981 aagattatca aaaattcgtt gctgaggatc tagatgatat cgacgtagag atatctgttg
    11041 cgaaacagag cttaaagcgt gataataaat atgaaccaga acaaaaggat taactctttt
    11101 gttcataaaa gaagactcgg aacattcaga tgatgtctgg ctgtttcgag tactttttct
    11161 aaagtagaga cattcatgta agaatcgaga acagattcaa caaggaggaa ttttttatgg
    11221 atcataaacc gactgaaaca aaagatgtta caccagttaa cgatacactt gaagatttat
    11281 tgaacaaccc gttttcaact ccaacagata cgctgaccac ttcacaacaa gcggagattg
    11341 atcagctcca agagcaacag acagctgctc gtttaattga caaactaccg gcggaacgcc
    11401 aagaacaagc taaacagcta gcagcaaaaa tcgatgctaa tgatgcacag tctgtcatca
    11461 gctacggatc ggctgcccaa gcaaaattaa gtgaattctc acaatcgatg ttgaatcatg
    11521 tgcaagcaca agatattggt cctgtaggag attccttaac tgaactgatg tatcgtctcc
    11581 aagaagccaa tcctgacgaa ctgcgtgccg gagaagggaa catcttccag cgtgtcttcg
    11641 ggaaggtcaa acaatcgatc tatgaagtga cagcaaaata tcaaaagatt ggtgcacaaa
    11701 ttgacaagat ttcagtgaaa ttagataaag aaaaagacgg tctgctaaaa gataatctga
    11761 tgttagaaca gctttaccag aaaaacaaag actactttga tgcactgaat atttatatcg
    11821 ctgctggaga attgaaaatg gaagaacttc aaacaacgat cattccagaa gcgatgaaaa
    11881 gagcagaaga aactggcgat cagatggatg ttcagattgc caatgattac acgcaattcc
    11941 tagatcgtct ggataaacgt acgcatgatc ttcgcttagc tagacagatc acgatccagc
    12001 aagcaccaca aatccgtttg atccaaaaca caaatcaggc cttggctgaa aagatccaag
    12061 catctatcgc tacagccatt ccgctgtgga aaaaccaagt cgtgatcgca ttgaccttgc
    12121 ttcgtcaaaa agatgcagta actgctcaac gccaagtctc tgaaacaaca aacgacttat
    12181 tgaagaaaaa ttcagaaatg ctgaaaatct cagccatcga aacagcgaaa gaaaatgaac
    12241 gtggaatcgt ggatatcgag acccttcaaa ccactcaaaa tgatttgatc gaaacaatcc
    12301 aagagacatt gcgtatccaa aaagaaggaa aagaaaaacg tcgtcatgca gaagtagagc
    12361 ttggacacat ggaagaagat ctgaaacaaa aactgttgga tttaacccaa tagatcacct
    12421 ttcgcttgtt actaaaacaa acgcccaaca acagtttatt acaactgttg atgggcgttt
    12481 ttcttatctt tcttctaact tctccattgg agtctatttc tcattcatct tgctgtgtcc
    12541 atagccatag ccaaaataaa tgatcagtcc aataaccatt acgataccga acacgatcca
    12601 tgtcacagtt tgcagacgaa gcatcaatac aatacaagtc aagatggaga cgattggcaa
    12661 tagcggaacg aatggcactt tgaattctcc agcttttggt ttgccaaaca tttttctcaa
    12721 cttcagtaag cctagactaa caagcatcaa ataggcgagc gccatgatat tcgttagttc
    12781 cgctaataca ttcaatggga aaaatccggc aaagaataca gctgccacac cagcggcata
    12841 agtcgctttt ttcggtgtac gatgcttttg atcgatttct gtcataaact taggaagtaa
    12901 gccatccttg ctgacagcaa acagtaaacg agcaagtgaa tacatcatcg agatcgttac
    12961 cgtcagtagc gttaaaattg cccctacagc gatcaccgcg cccactgttc catgtcctac
    13021 atacctcatt gcaaaagcca ctgggtcatt gactcctagt tcagtatacg gaacgattcc
    13081 tgtcaagatc agcgtcacga tgatgtaaag gacagtagca atcagtatcg aaccaatgat
    13141 tcctcttggc acatcttttt gcgggttttt gacttcctcg gctgccattg caacggcatc
    13201 aaatcccaag aaagcaaaaa acacaagagc tgcaccatct agcacaccgg aaaaaccaaa
    13261 aggcatgaat ggctgccagt tatcaggttt aacgaaaaat attcctacaa tgataaacaa
    13321 cgcgatgata ccaaatttga catataccat ggcattattc aaacgtaatg ctttcttggc
    13381 ttcaagcgac acgatgtaag tgacgaatat cactacgagc accgcaatca agtcaatgta
    13441 tgtaccattt tccggattgt acccgccagt taatgcttgt ggaagttcgg cccctaaact
    13501 tttcaaaaaa ccttgcatat agccagacca tccggaagca acagaggaaa ccgctagtaa
    13561 aaattccccg atcaacaacc accctgtcag ccaggcagca aattcaccaa atacaacata
    13621 cagataggca tatggcccac caatcactgg cactcgcgaa gcaaattctg cgaaacttag
    13681 tccagacaaa atgacaacga ttgcagctaa gacaaatgaa agtgacagtg ctggaccagc
    13741 attttgattg gccgcaaccc ctgtcaccac aaatattccc gtaccgatga tcgcaccaat
    13801 tccaagcatg atcaaatcag acgtttttag ttcttttttc atctcactcg tatgattgag
    13861 attgatctct ttcttacgaa acaaatccat tgaagttcct ccccatttta ggttggaaaa
    13921 cagcgattaa actgttttca tataccctaa aaggatacca cctaaaatga ctaaaaggca
    13981 accaacaata acataaatga gctctttttt cgtttttctt tcgccaagga tgaaaatccc
    14041 acctaaagta gagataatga tccccatttg tgaaagtgag aagccaacag ctaaaccaag
    14101 tgatttgact gttagaagca tgcagatgtt tcctaagccc cataataatc cacagatcat
    14161 gtttttccac acaaactgat cgactttaac tttacggaaa gcaaagaagc ttgcgcctaa
    14221 gatcattccg atactttgag gtaagataat tgacatcgca tccaaattcg cccagttgac
    14281 gatgatggta tatacaccat aaccaatcgt agagaagatc aatgctctaa aacccttacc
    14341 aaaatcaagc attttattat ctgtatctat ttttccttcg tcatcttgtc gggcagtcaa
    14401 atacgcaccg cttacaagaa gagctaaagc aataataccc tatacataat ctttcgtttg
    14461 tgtccattca tggaagaaaa cggctcccgc aatcgtattc aaaattaatt gaaaaccagt
    14521 agacaatggt agccctacag aaacacccat gtacttcatt ccatggaact gtccattttg
    14581 tccaacactc caagctaagc cggataaaaa tccgatcaag aaaatccaag gggtgattac
    14641 aggactaaca acgaaaaaga caactaaaga aaacagaaat gcaccgatcg tcattcctag
    14701 tgtttgttga ttggcatcgc caccaatttt accacttact aaaccaatac taccccatgc
    14761 caacataggg actaatgcta ttaaaatttc cataaccaat ctccttttat ttcacatgat
    14821 aagaaactta tcagcgaaaa cgttaacaac acaagcactt tttttcgttt ctaacgattt
    14881 tctaacgttt ttcattataa aaatttaaga ttgggaaaaa taaagtcatt gttgtccctt
    14941 ttccttcagt tgattcagcc gtcaaatgga tacctaattt ttcggccaga ttttttgcta
    15001 aataaagtcc taatcctgtt gaatgtgttt tgctaagtcg accatttctc ccagtaaatc
    15061 ccttatcaaa gatccgtttc aagtcttccg ctgggatacc gatccctgaa tctttaactg
    15121 aaaggctgac tccttgcgct tccttttcta tgctcacaat gatgtttccg tgatccggcg
    15181 tatatttgat ggcattacta agcaattggt tgaagataaa agcgatccat ttagcatcgg
    15241 tcaaaacagt atgatcctca ccaacgatct cgtagcgaat atttttttgg ataaaataat
    15301 ttgcctgact gcgtatcaca ggctgaatga ttccttttag tccatattct tggatgaggt
    15361 agtcccgcga gaaactgtcg agtcttgcat aatacaaaac ttgttctacg tattcatcga
    15421 tttttgataa ttcattttct agtaatgtat acttttgatc gtcaatatcg aactcgatcg
    15481 aacgaactag caatttagaa gcggaaagtg gcactttgat ttcatggacc catgagtcaa
    15541 tataatcttt ttggtcttgt tgattactga ttgctgattg cattacttct tggtgttcac
    15601 ggatcagctg attgatatat tcttctacca gtttttcttc ttctgtcctc ccaccactca
    15661 aataattttg caaaggggaa gttttttgta tgtcggctaa ttttccccac catcttctct
    15721 tgctcaagta accacccagc agataaaaaa tcagaaagac gccctcaatc aaagctaaat
    15781 aagcaattgt gctcaaatca actttgatgt ttggcgccag ccacatcaca aagatcgtca
    15841 atgcaataaa aaacagccat cccaagatca atgtccactg atctttcaaa tatttaaaaa
    15901 aattcatctt aaccgctcat cctttcaggg aacgatatat ccctgtccta cttttgtttc
    15961 gatatatcct tggatacctg cctgttcgat ttttcggcgt aaccgattga tgttgactgt
    16021 caatgtattg tcatctacaa atcgttcgtc atcccacagt gctcgcagca atttttcccg
    16081 acttaagatc tttccattgt gcttcatcaa aataaataat agacgatatt catttttgct
    16141 caaatcaatg atctctccgt tgatttccat acttccgttg tcaacattca acgtaatgcc
    16201 attgtggctc atcatttcac tagataattc tgtgtagttg taagaacggc gaagtaatgc
    16261 attgattttt gcaatcagta catcgatttg gaaatgtttt gtcacaaaat catctgcccc
    16321 catgttcata gccatgatca tatccatatt cgtattcctg ctggagataa aaatgatcgg
    16381 tactttcgat acttcacgta ttttttgaca ccaataatag ccatccaaaa cgggaagatt
    16441 aatatctagt aatacaagct gtggttcttc tctttcaaag tctgagaaca cttgattgaa
    16501 atcggtcgtt ccaaaggttt caaattgcca tttctcaagt tcctccatga tcaattgtcg
    16561 aatgatctct tcgtcttcga ctaccatgat ttttgccata aaaaatccct ccatctttct
    16621 tcttgctact acaatatcaa agaagccctt gtgaaacaag ttggcttacc tggaaaatgt
    16681 cattttagca aacgttaaaa aaaactggaa atatgcgatt taatcgcgtt tgtctcttcc
    16741 aagcagctta aatttattat gttattctta ataattggtc tattatctgt tatttttctt
    16801 tcgttgtttt tgtcttttaa attttcgata acaccaataa ccgaaaagcg ttagcactag
    16861 caatatacct agaaggagta taagtaatcg ataaagatgg tatttttttg tctgttctat
    16921 ctttgaagag acttcttctg taaagggaac tcgtcttcct gtcactagta atcggtgagt
    16981 gttcacggtg taaggtgtac aagtgataag tgtgactaga tcctgctttt ctctaattgc
    17041 taatgtattt gtttttgttg gaaggacaac aattttctca acgacttcat aagcgagcat
    17101 ccgaccatac acttcaagat agaatcggtc atcttgctcc atttcttcaa gatccgtaaa
    17161 gagttttttc tcagcaatac cgctatgggc ggtaatcacg gaatgtgtac tctctcctcc
    17221 agttggaaaa gaggtgcctt gcaggactgt cgctcctcta tctaacaaaa gatcattcgt
    17281 ttcatcaaat aaaggcaagc taacattgat tttcggaata tagatggctc cgatcatatg
    17341 ttgttcgtaa tattcttttc ctggattttg gacatttcta accgcttgct caaaaggatc
    17401 tttgacttgt cccattcctg ggattactga tgtatgagca agcgtctgat ttttcttttc
    17461 atgcactgct aattgttgct catgtttttc ttctttatcg cgttcaagct gcttttgata
    17521 attctcgatc cttctttgat ccaaataatt gctgagagca tctgctaaaa atggatatgt
    17581 aaaaactgca gctccggcaa gaaacatcac tgccataaat aattgtaata aaagactgat
    17641 ttgggtttga ggtctattac ctattttctt tttcattttc ttcaccttct tatttaaaaa
    17701 aactgcagca acctttataa aaccgattgc tgcagcttat ttttgttgct tatacttcag
    17761 acttcatttt agatttacgg taccagctgt acgctccaat catcaaactt attccgatta
    17821 gtaagaatgc caagagcccg ttcccaccag tagcaggaag tgacccgcgt ttcatattcg
    17881 gtactctttc tggccctgga ttttctactc ctacgatctg aatttcttca ttatagcttc
    17941 cataagagat gataaattca gtaaaggctg cctcacctgt tggaagtaca taaccttctg
    18001 gcgccatggt ctctttcatt tgataggttc cataatctaa cccttgaacc tcaaagtatc
    18061 catttccatc tgcgacaaat tctgttgctt gtccgcttgt tgcccactca attctatctg
    18121 ctcctgattc ataaacagct aaaggcgact cccctttata aaatattgcg tattcagaac
    18181 ggtcttcatt ccacaattcg aatcttgcat ctttaagcaa ttcactgctt gatgcatcat
    18241 atttttcaaa ctttcttcca cctgttgtga ctttctctgg cggctctact ccttcttcat
    18301 aatcaaattc atcatttcgt ccgatttcaa taaccgctgt gttgttatgg aaatcatccg
    18361 cttctagttc tgtggaaacc ttcaaattat acgtgatcgt taaatctcct ccacgtgcat
    18421 ctgctaatct acctaaagta tctactttgg ctactccatt ttcttctgtc aaaatcgtca
    18481 cgacaaaccc gttgcttact acttctacta tataatcttg tccttttgtt aaaatcgtct
    18541 cgccggctcg cacttctatt tcctgatcat aataagttag accaactgta ggcgtatctg
    18601 tgattttgta atttttgata acagcgcttc cgttttcaag gacagaatcg atgaaataag
    18661 gaataggtgc tgtgatcgtg taagaagccg tttttcctct ttctagatca atataagaaa
    18721 tcgtttcttg ttctccagct tcattaataa tcgttacttg tctaaggtcg ctttcctcta
    18781 cattcatctg tttatctgct tctctgattt cgtttttcgg atacaaatgc acatctgtat
    18841 tgtatgtgtc tttccatgta gaaccatccc acacgccatc caccatatca ggcatcatca
    18901 ctggcatcat aacaaccatt ggagacgcga ttctggaaat atgagctggc gaataagttt
    18961 ctaagaaaag atagacttta tctctagaag aaggtgcttc ttccgtcact tggacggtag
    19021 agaataccgc ttctccagct tcattcgttg tttgtttgtc aatacttgtc agtcgatatt
    19081 ttaaaaacca actttctgtg tgccgttttt gaatccattc aatcgcttcg gcagaattaa
    19141 gaccgtcttc tcgttccggt gttaaaggat tatctcgaat caaatcgtaa tatacttccg
    19201 ttacgtcaaa caaatcaaaa tcgactcctg gaatattttc ttcaccaaaa tccgtagaaa
    19261 ccaatccgct attcggtcgt tcttccggaa cagatgagaa accttttttg tgtaatgtaa
    19321 tcgtcacttc acttggtttt tgtgcgactt cttcggcttt gactagtttt ccgttcccga
    19381 aaatccctat tcccgcagta acgagtaaca gtacactcat tcccgtgaca atcatttttt
    19441 tattcatttt tttctccccg ttcttttttt att
//
LOCUS       DACDRK010000045        19410 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDRK010000045 DACDRK010000000
VERSION     DACDRK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.045"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..1441)
                     /gene="brnQ"
                     /locus_tag="IWJ77_001746"
     CDS             complement(62..1441)
                     /gene="brnQ"
                     /locus_tag="IWJ77_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9453347.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            2147..3118
                     /locus_tag="IWJ77_001747"
     CDS             2147..3118
                     /locus_tag="IWJ77_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9453348.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            3142..5334
                     /locus_tag="IWJ77_001748"
     CDS             3142..5334
                     /locus_tag="IWJ77_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9453349.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            5351..5827
                     /gene="ybeY"
                     /locus_tag="IWJ77_001749"
     CDS             5351..5827
                     /gene="ybeY"
                     /locus_tag="IWJ77_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9453350.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            5805..6206
                     /locus_tag="IWJ77_001750"
     CDS             5805..6206
                     /locus_tag="IWJ77_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9453351.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            6221..7120
                     /locus_tag="IWJ77_001751"
     CDS             6221..7120
                     /locus_tag="IWJ77_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9453352.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            7263..8075
                     /gene="recO"
                     /locus_tag="IWJ77_001752"
     CDS             7263..8075
                     /gene="recO"
                     /locus_tag="IWJ77_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9453353.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            8459..9376
                     /gene="glyQ"
                     /locus_tag="IWJ77_001753"
     CDS             8459..9376
                     /gene="glyQ"
                     /locus_tag="IWJ77_001753"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9453354.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            9378..11453
                     /locus_tag="IWJ77_001754"
     CDS             9378..11453
                     /locus_tag="IWJ77_001754"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9453355.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            11837..12616
                     /locus_tag="IWJ77_001755"
     CDS             11837..12616
                     /locus_tag="IWJ77_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453356.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            12657..13601
                     /locus_tag="IWJ77_001756"
     CDS             12657..13601
                     /locus_tag="IWJ77_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9453357.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            13617..14396
                     /locus_tag="IWJ77_001757"
     CDS             13617..14396
                     /locus_tag="IWJ77_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9453358.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMIDTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            14409..15107
                     /locus_tag="IWJ77_001758"
     CDS             14409..15107
                     /locus_tag="IWJ77_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9453359.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            15135..15899
                     /locus_tag="IWJ77_001759"
     CDS             15135..15899
                     /locus_tag="IWJ77_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9453360.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            15912..17729
                     /locus_tag="IWJ77_001760"
     CDS             15912..17729
                     /locus_tag="IWJ77_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9453361.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAPHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            17743..18966
                     /locus_tag="IWJ77_001761"
     CDS             17743..18966
                     /locus_tag="IWJ77_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HAP9453362.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRYLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            18980..>19410
                     /locus_tag="IWJ77_001762"
     CDS             18980..>19410
                     /locus_tag="IWJ77_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9453363.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVG"
BASE COUNT         6622 a         3494 c         4057 g         5237 t
ORIGIN      
        1 ctttcttcta gctgtttttt tcctgtttaa tatctcttat gttaaattgt gtttgatttg
       61 gttattcttc tggtaactca cttgcttctt gtttatatcc tgatacgcga tatacataat
      121 aaaagagcaa gctgccagcg taaccaagca atgttgggac gacccagcca agacctaatg
      181 cagcaaaagg aacataatga tgataaaatc ctagtaagct tgctacgaca gtttgctgac
      241 tcactaatgc aggcgaattt gccaacatat ccaatacggc tggaacggca gaaaagaaca
      301 tcgttacttg atagacaaat ggactctttt ggaagaattt tgctgttaag gaaagtaaga
      361 ttaacgcaag tgacaatgga tacaaaagca tcaatactgg taaagaccat tggatgatat
      421 tatccaatcc ggcattcgca acgacaaaag agacaaaagt cgttaaccgc agccatgtca
      481 tataactgac tttagggaac agtttatgga aatcttgtgc aaaagaaaca acgagtccca
      541 tagcagtagt gaagacacct aatgtgacca ttacccctaa gaagctagca ccgaagttac
      601 ctaaataaaa attagtgatc tgagaaagtg cagttccgcc attttctgat aaagacatct
      661 tattcaaact catcgcaccg agaaggacca gaccaaaata aatcatgact tctaacgcaa
      721 cgctcaggct accagctttt gctgttaatt ctgtgacttt tttatcttta aaaccaagat
      781 cacgaactgc atggacaaaa gtgacactta aagcaagtaa agctactgca tcaaccgtgt
      841 tgtatccttc taaaacgcca tttgtcaaag cgttcgtctg ataagaatct gcagcagatt
      901 ggtttagatt ccccatgggt tggaataaag aaacgacaaa aatgatagct aataatgcaa
      961 gaaacaagga gtttaaatac tttccgacat atttgaccaa acgtgttgga tgtacagtaa
     1021 ggaaataagc aagtaaaaag aaaagagcag agaataaaaa catacctaat gaatcatatt
     1081 tttcaggcaa aaagggttta gcagccattt caaaagcagt tgctgctgtc cgcggtgtac
     1141 caaagaatgg tccgatcgtt aaatgaacaa ggatcaagaa caaggcagca taccattttc
     1201 cgacaggttt agctaagtca tacagaccat cactttttgt cactactaca gctaagatag
     1261 ctaataatgg gaaaagagcg cctgagatgg caaatcctac accagcgctg atccagtttg
     1321 atccagccat ttgacctaag tgtacaggaa agatcagatt acctgacccg aagaacatcc
     1381 cgaagataag tgaactgacc atcaataatt gcttccatga ataagttttt gtttcttcca
     1441 taaatttttc ctccaataat tatcattttg agtaacaaaa taaacgttgc agaaactaga
     1501 aataccacta atatcagatt ccatgaaaac caccacctta aaatataaaa aaagcccttt
     1561 tccacttggg aaaagggctc tagattctag aacggtacca ccttttttcg cttaaaaaag
     1621 cttcttcatt cacggacgct tttcgataaa gttatccgct agcctgataa tggaggcagc
     1681 cacaatgatc tacgttttcg acattgactc ggaaatgatg ttcgtctatt cgatttattg
     1741 tcatttctca cttacatgac tcactgggaa taaatcactg aatagcctac tgttttcctc
     1801 gacgctttgc tgtatattta tttgttgacg ttataataca cctgtttttt ttgaaatgca
     1861 agaatatttc aaatattttg ttttcttttt ttgaaacaag aaaaataccc agagaatact
     1921 tgctaagtta gacggaatac ttgtcctcta tcttatgcat agcttacatg atcagctttt
     1981 tttattcaaa cgagaccttt agagaatgga agcggctagt tttttaacgt ttcttggtac
     2041 ttatggttaa gcaactcttt tagaaccttt tcttatttgc tatatagttt cttctgagta
     2101 tggtaaaata ggaaagaatt aatcgaaaaa aaggggatgt ctatttttga cggaagagtc
     2161 tagttcttta gagattagat tgacggctgc tgatgatgtc agcatgcttt taggtgcaca
     2221 cgacaagcat ataaaactga ttgaggaaac aacggaaaca accatccata cacgtggaga
     2281 aatcattcag atcattggtg agcaatccca gatttccttg gctgcctcag tcattcggac
     2341 attacaggaa ttgatcaaac gaggaatcca cgtaagtacg cccgatgttg tgacggcatt
     2401 gaaaatgggg caaaaaggta cattagatta ttttgttgag atgtatgagg aagagatcgt
     2461 caaagaccgt aatggaaaac ctatacgggt aaaaaacagc ggccaaaaga aatatgtcga
     2521 atcggtagcc aaacatgata tcgtatttgg cgtaggacca gccgggacag gaaaaacttt
     2581 tcttgcagta gtattagcaa tcgctgcctt gaaaaaaggt gaagtgcaaa aaatcatttt
     2641 gacgcggcct gctgtggaag caggtgaaaa tctaggcttt cttcccgggg atctaaaaga
     2701 aaaagtggat ccttatctgc ggcctgttta tgatgcttta taccaaattt ttggattgga
     2761 tcatacgaac cgattgatgg aacgaggcgt aatcgaaatc gcacctttag cttacatgcg
     2821 ggggcgaaca ttggatgatg cattcgttat cttggacgaa gcacaaaaca cgacagtcgc
     2881 tcagatgaaa atgttcttga cccgtctcgg ttatcaatca aaaatgattg tcaatgggga
     2941 tacaagtcag atagacttgc ctcgagggac gacaagtggt cttgttcatg cagaacgaac
     3001 attaaagcaa atcaagaaaa tcgattttgt taattttgaa gcaagtgacg ttgttcggca
     3061 ccctgtagtt gccgaaatca tcaaagctta tgaaaaggca gatctccatc aagaataagc
     3121 tttgcttagg aggataagca catgttgaat aaaacactgc agaaactaca agaaaaactt
     3181 ggaaaggcag cattgccttt cctgctgttc ttgtttttta tcattatctg cggcgttaca
     3241 tttagtagtg tcaaacaaaa ggccaatgat ttcagagaag gccaagtagc agaagaaagt
     3301 atccgtgcga acaaaacgat cgagaacacg gaagaaaccg aacaaaaaag aaaactggca
     3361 gcagaagcag tcactccaga atacacttac caaaaagatc ttgcagatga ccagaacaat
     3421 cggatcaagc aactatttga attgatcgat aagacgaatg atgccatcaa caaatcttat
     3481 gacgaaaaga ttgataaggc aaaggaagat gaaaatatcc caaagcctac tacagaagag
     3541 cggatcgccg cgctgaagaa aaattttgaa aatgtgaatg cagaaaatgt tgtgttttat
     3601 caaaaactac cgaacaattt ttattcgacg atatttgaaa tgactgaaac tgagattcat
     3661 actgtgaggg atgagagtct gaagctcatt gacgaacaga tgagcaaaca agttcgtgaa
     3721 agtgaattgg aagcttttaa acaagaagca gaagagcaaa tccaatattt gaatgtcaca
     3781 ccagcacaac aacaaatgat tcgctactta gtggatcaag gtatcgtagt gaatgacgta
     3841 ttgaatgaga aaaagacaga agaattgaaa caatccgcac gagaagctgt acaaccagtg
     3901 atgatcttcc aaggagaaat cattgttcgt gaaggtaatc aaatcgatgc ttcagctatg
     3961 aaaaaattgg aattactagg actgactaat caaacaactt cgatttttcc gctaattgcc
     4021 atggttttag ctgttttgct tcaaatcggc gtactgattt ataattcact tcaattccaa
     4081 aatgcaggag aaagaacaaa atacgtgctt ttctatgtga cagctatgtc agtcagcata
     4141 gtgctgatga aatttttcca attattccaa acggaacaag ctacttatat cccgctgttt
     4201 tatcccgctg cttttgcacc acttgtttta agctttttct tgaatcgaag agcagggatc
     4261 atggctggta ttttccaagc agtatccgca cttttcattt tttatgattc gattggcacg
     4321 aactttctaa cgattatctt gatgtcttat gttttctctg ggctcatggc aacgattgtc
     4381 agacgcaaac ggatctcaga acaaggatta tctgctgcca tgtgggtcat tatttttcct
     4441 gtgatgatgg atgtcatttt gatcgtctat caaggaatga gctttggtga tgcaacgact
     4501 tggttaatgc tcgtctgcgg gttcactggt gcactcttct cattcttgct gacgatcggt
     4561 ctacacccat atattgaatt gatggtcaat gatgacagtg taattgtatt aaatgaactc
     4621 agcaatccaa atcatccttt attgaaacag ttgctggaag aagcgccagg tacttatcat
     4681 catagtatga tggtagctaa tctaagtgct aacgctgtag cagaaatcgg cggacgttct
     4741 ttgctaactc gagtagcttg ctattatcat gatatcggca agatcaaaca tgctagcttt
     4801 ttcgttgaaa acttgccttc aggagcggaa aacccgcaca atttcttatt gccagaagac
     4861 agtaaacata ttatctttgg acatgtgaca gatggagcca aaatattgga agattataat
     4921 atgccgcaaa tggtgatcga tatttgcaaa cagcatcatg gaaccacgct aatgcgttat
     4981 ttctacatca aagcgaaaga acgcaatccg gaagtaacag aagagcaatt ccgttatcct
     5041 ggaccaaaac cgcaaagtcg tgaagcaggg atcgtcaata ttgctgacag ttgtgaggct
     5101 gcagtacggg caatggatca tccgacgagc gaaaaaatcg aaagttttgt ccataatctt
     5161 atcgaagagc gtatttccga tggtcaatta gacgatagtg gactgacctt gaaagaaatc
     5221 cgaaaagtgg aaaaatcatt aatcagcggt ttaagtagca ccttccattc tcggatcaaa
     5281 tatcctaaaa tgaaaacaga agttgagaag ataaaggaag tacaagaaga atagaaagaa
     5341 gcgatgtata atggatatta cctttatgga tgaaacaaac caagtctcag aagagaaaat
     5401 gaaagaaatc gatgacttgc tgcaatttgc agcatcttat ttaaagctgc cagaggaaac
     5461 agaaatgtct gttactttca tggacaatgc ggcaattcaa gtaatcaatc gtgattaccg
     5521 tggaaaagat gcaccgacag acgtcatcag ttttgctctg gaagaagaag gcgaagacga
     5581 aatcccagtg atttttgaag atggagagaa tccattacct cgtgtactag gagacatcat
     5641 gatctctatt gaacgtgctg ctgaacaagc agaagcttac ggccattcat atgatcggga
     5701 acttggcttt ttagcggtac atggattttt gcatatcaac gggtatgacc atatgacacc
     5761 agaagatgag aaagaaatgt ttggtttaca gaaagagatc ttggatgcct atggactcaa
     5821 aagataaaaa cgtagagaaa aacaaacatt tcctgatgtc tgtggaattt gctgtccaag
     5881 gagtcaaaac cgtttttgag gaagaacgca atatgaggaa acatgttgcg ttcggtatcc
     5941 ttgctttatt tatgggcgtg attttccagc ttgatcggat agagtggtta tggctgcttt
     6001 tagctgtttt tctcgtttgg atagtagaga tcatcaatac tgtttttgaa aatgtagtgg
     6061 acatgtttac ggattttcat tttcatccga ttgggaaaaa aatcaaagat atggcagctg
     6121 gagcagtttt agtgaccgct tgttttgctg tcatcattgg gttgatttta tttgtcccta
     6181 aaatatatca tttgttgttt aattaaaaag gagaacgtat atgccagaac ataaatcagg
     6241 gtttgtcgca atcgtcggcc gcccgaatgt aggaaaatcc acattattga accgaatcgt
     6301 gggtcaaaaa attgccatta tgagtgacaa ggcacagaca actagaaata aaatccaagg
     6361 agtatatact acaccagaag ctcagctgat ttttatcgat acaccaggaa tccataaacc
     6421 aaagcatcgt ctaggagatt ttatggtaga agcagcttac agtgcgttga aagaagtgga
     6481 tgcagtctta tttatgatca gtgctgatca aaaacgtggc cggggagacg attttattat
     6541 tgaacgtcta aaaaacgtgc attctcctgt ttatctgatt atcaataaaa tcgataagat
     6601 ccatccagac gagttgctgg gaatcatcga agattattcg acgcagatgc cttttgcaca
     6661 agtgattccg atctctgcta cagaaggaaa caatgtcgaa cgcttaatgg atgtattagt
     6721 cagtgaaatg ccagagggcc cacaatattt tccagatgat caagtgactg atcatcctga
     6781 atacttcatc gtttctgaat tgatccgtga gaaagtcttg tttttaacac gagacgaagt
     6841 gcctcattct gtcgcagtcg ttgttgattc gatgaagcga aacgagaatg ataaaatcca
     6901 tatccaggcc acgatcatcg tcgaaagaga tagccaaaaa ggaatcatta tcggcaaagg
     6961 cggaaaaatg ctgaagcaag taggaacgaa agcaagaaag gatatcgaaa atcttttagg
     7021 agacaaagta tttctagaac tttgggtcaa agtccaaaaa gactggcgag acaaaaaagt
     7081 ctatctgcaa gacttcggct accgaaaaga cgagtactga tattctggac ttcttatagg
     7141 ttaggtatag aaataaactg gataaaaatc cgtacgttga gaaaaacagc ggaagatcga
     7201 aacataaata atctggatcg gcagaatctg cttttccaga tttatttttt aggagggaga
     7261 atatgagcca gcttgtagaa tcgaaaggct tgattttatc tagccgtaat tacaaagaaa
     7321 aagataaatt agtcaaaatc tttacggaat cttctggcaa aatgatgttt tatatcaaag
     7381 gaatccatcg gcaaaatcag ccattagctc cggctttgct cccctttacc gaagcagtct
     7441 atatcggtaa ttttcgaagt gaaggtttgt cgtttttaag cagtgtcaaa gacgtccatg
     7501 cgtttcggaa catccaagaa gatatctttt tgagcggtta tggtacgtat ttgctaaatc
     7561 tagtagatgc agcaatcgaa gatcatcaat atgacccgca tttattccaa tttacgcaac
     7621 aagctctgca gcggatggac caaggagatg atgcagaaat catcacgaat atctttgaag
     7681 tgcaaatact gcaacgcttc ggaatcgcgc caatttggac gcactgtgtc gtatgcgggg
     7741 aaacaaaagg aaaatttgac tattcttcca aatatggcgg tgtgatatgc gagaaacact
     7801 ggccaatgga cgaacaccgt tatcatgcag atcctcgcgc tgtttatttc gttcgaatgt
     7861 tttcggctat ttcttatgat aagatcagtg gaatccactt gaaagaagaa acgaagcagg
     7921 caatccgtga cctcattgac cagttgtatg atgaatatgt cggtattcat ctaaaaagca
     7981 aaaagttcat tgatcaaatg aagtcttggg cacacgtatt aaagacgcca gagaaaaata
     8041 tgcctgaaaa agagatacct gaaaaagaag aataaatgaa agatacaaca aaaaaatcaa
     8101 aactatttga caaatacagg gacttccgtc tattattaac cgtagattaa atagatcgtt
     8161 cactgagagg atctattata caaaccagaa aactgcaaaa tgaatacgta cattgaccaa
     8221 gatcagtaaa aaacacgtgg tttagcgagt tcgggagagt ggaagccgga cccaaacgat
     8281 ttttgaagag gcacttgcga gtacgataaa tgaagctgcc gaatctttat tcggaagtag
     8341 ggtggaaccg cgaaattatt cgtccctatg tctgtaatca gacataggga ctttttgtcg
     8401 ttttctttcc tgtattctta ttgacagcaa cgcttaaaaa ttgaaagggg caaaacagat
     8461 gagtaaaaaa ttgaccgtcc aagatatgat tttaacatta caaaaatttt ggtcatcaaa
     8521 cggctgcatg ctgatgcaag cttatgatac agaaaaagga gcaggtacga tgagcccata
     8581 tacattctta cgggcaatcg gaccggaacc atggaatgct gcttatgtag agccatcccg
     8641 ccgaccagct gacgggcgtt atggagaaaa cccaaaccgt ttgtatcagc atcatcaatt
     8701 tcaagtcgta atgaaacctt cacctgaaaa tatccaagag ctttatttag aaagcttgcg
     8761 tcttttagga attgatccat tagaacacga tattcgcttt gttgaagaca actgggagaa
     8821 cccatcgatg ggttgtgccg gtgtgggctg ggaagtatgg cttgatggga tggagatcac
     8881 acaattcacg tacttccagc aagtcggagg actaccttgt aaaccagtta cttctgagat
     8941 cacttatgga ctagaacgtc ttgcttctta tatccaagaa gttgaaagtg tttatgatct
     9001 ggaatgggct gatggcgtga aatatggtga gatcttcaaa caaccagaat atgaacattc
     9061 taaatattct tttgaagtaa gcgaccaaga cctgctacta agcaattttg atcgttttga
     9121 aaaagaagct aaacgctgta tcgatgaaaa tcttgttcat ccagcgtatg attatatttt
     9181 gaaatgtagc catacattca atttattaga tgctcgcggt gcagtatccg taacagagcg
     9241 tgcaggttat ctttcccgta tccgcaacat ggctcgtgct gtagccaaaa tctttgtggc
     9301 agaacgcgaa aaattaggat tccctttatt gaataaaaac acagcaaatc cagaaaacgt
     9361 gaaggaggct gaataagatg gcaaaagact tattacttga aatcgggctg gaagaaatgc
     9421 ctgctcatgt agtcacacca agcatgaaac aattagaaga aaaaacagca aaatttttag
     9481 atgaaaacca attgacttat gatacgatcg aaacgttttc tacaccgcgc cgcttagcaa
     9541 tcaaagtaac aaacattcca gaacgtcaag cagacagcga agaagaagtc aaaggaccag
     9601 ccaaaaaaat tgctttggac gcagatggaa actggacgaa agcagctgaa ggatttgtcc
     9661 gcggacaagg tctgacgaca gatgctatca cttttagaga attaaaaggc gtggaatatg
     9721 tctatgtaac gaaatacact cacggcaaag aagcaaaaga agtattgaca ggtttagcag
     9781 atgtcattaa aagcctcact ttcccagtaa cgatgcactg ggcagaatac gattttgaat
     9841 atattcgccc tattcactgg atcgtggcat tgttagatga cgaaataatc ccgtttgaga
     9901 tcttggatgt caaaacagac agagtttcaa gaggtcatcg tttcttaggc gaagatgtga
     9961 catttacgca tgcaaaagaa tacgaagaaa aattgaatga acaatttgtt attgcttcac
    10021 cgcaaaaaag aaaagaaatg atcatcacac aagcaaacga attagcagaa aaaaatcact
    10081 ggatcattga tctagatgaa gatttactag aagaagtcaa caatctagtc gaatatccaa
    10141 ctgtcttttc tggcgacttc gaagaaaaat atctatctgt tcctgaagaa gtactagtca
    10201 cttctatgaa agaacatcaa cgttatttcg aagtacggaa cgatcaaggt atgttgcttc
    10261 ctcattttat ttctgctcgt aatggcgatc gggtaaaatt ggaaaatgtg gtacgaggca
    10321 atcaaaaagt attgactgct cgtttagaag atgcggaatt cttttataat gaagacaaga
    10381 aagcttcaat cgaccaatac gttgaaaaat taaaaactgt tactttccat gaaaaaatcg
    10441 gtagcatcta tgagaaaatg caacgggtac aagtcatcgc tcgcatcata ggcgaacaag
    10501 taggactatc tgaggaagaa ttgatggatc tgcaacgcgc tgctgaaatc tataaatttg
    10561 atctagtaac caacatggta ggagaattcc ctgaactaca agggatcatg ggagaaaaat
    10621 atgctctgct taaaggcgag aaaccagccg tagcaacagc gatccgtgaa cattacatgc
    10681 ctatatccag tgaaggagat ttacctgaga ctacagtagg ttctgtttta gcgattgctg
    10741 ataaattaga tagtatcttt tctttctttg cagttgggat gatcccaagt ggctcaaacg
    10801 atccatatgc tttgcgccgt caagcatatg gtgtcgttcg aatcatcgaa tcgaaaaaat
    10861 gggcctttcc tttgtctgtt ctgcaagaaa cgattagtga agttatcagt aaagatacgg
    10921 atcgttttgg cattggcttg tcagcgggac aacaacaggt gatcgacttt atcaaaggcc
    10981 gtctgcgcca attattgaca acgaagaata tccgtcatga tgtcatcgaa gctgttttga
    11041 atgcagaaca aaaagatctg acaaaagtat ttgcagcagc acaattgttc aaacaacatc
    11101 ttgcagatga agacttcaaa ccttcaatgg aagcattgac acgtgtcgtg aacttagcga
    11161 aaaaagcgca gcttgaaaaa caatcagaag tagaccctga gctgttcgaa aacgacgcag
    11221 agaaagaatt acacaaagca gtagaagcaa tgaaagcaag ttttgctgac caaacgatcc
    11281 acgaaaacta cgaccagctt gtcgctcttc gtccactgat cgaaaattat tttgatcaaa
    11341 cgatggtcat ggctgatgaa ccagcagtac gtaagaaccg actaacacaa cttagtcaaa
    11401 taactgccat ggctttgtca ttagcaagct tggatcaaat catcacaaaa tagtcttttg
    11461 tttgaaaaac agagagcatg cacgaaacgt gctgtgtctc tgtttttttg ttataaatat
    11521 aaaagctgtt attaattcta ttttaataat ttttgttatt ttattctttg ctagaatttt
    11581 caaaacgctc atgaaaagtg aggaaataaa aatgaaaaag attaataata atcttgttac
    11641 tgttttattt acttttaaat taggaatttc cctagtcaag aaaactgttt gtggtatgat
    11701 tatataggga aaacctgttt ataaatattt ctatgaatat attagtttaa ttatgctttt
    11761 aaaataaaaa gcgctcattc tgatgtgaat gaatcacatc tttatttatc tatcatcaat
    11821 atttaggagt gaatctatgg aaaagccgtt cgtcaagtta ttggctactg tggcggtagg
    11881 cacaggcgct attgtgattt gcttgtttgg ataccatctc aataatcagc ggcaacatca
    11941 tcaacggatc aattacgcag aatctgccat caccaatcaa aaagacaccg tgacctcttt
    12001 atcaaaagaa gtagataagc tgtacagtac aaaagaaaaa attttcctaa atcctgaaat
    12061 aacagaagaa acagtttcaa atttgtcaca taaactatct tctatcaaac tttcagcaga
    12121 tgattatgac atcaaggaat cagaattacc taaagaagct gctgcgatcc aagaagaaaa
    12181 gaaagcagtc ttaacacagt tagaggatgc agaatcaaaa ctgaagatcc aaacggctgt
    12241 caataaatta tttactaaaa acgtatcaaa ctggcagcaa gctgtggatg atgtcatcat
    12301 caaagaaaag ttggcaagtg cagatgtcgc ccatgtacgt gaaaatatga gcttttttaa
    12361 ggatagtgct tggaaaacgg tcgttatgca atatctagga ttcgctgata cccaaattgc
    12421 acaagtcacg caactagatc agctattcga tacgatgctg aaagacggtc aagtaacagc
    12481 tactgctact tacgatcaat atttgaccgc actcagtctc atcgaacaga tccggaatga
    12541 aaaaataaga gccgcatacg cgtcaaaagc agaaacagtc gctcagcaaa tgggctatag
    12601 caatacgagc tattaataag cgagaattca agcaaggcta gcaaacggag gaaactatgg
    12661 caaaacgaag aagaagatca agagggaaaa agatatttct aattatttta gcaattcttg
    12721 tcgtattagt attagcaatc gtaggagtag gagcaaaatt atatctagat ctctcacatt
    12781 ctattgaaaa aacttacgaa tcagtggaac gaacccaagg agaacgaaca aaaccagttg
    12841 atctggacaa acaagaatct ttctctgttc tattgatggg gattgatacg ggagatttag
    12901 gtcgggtcga tcaaggaaga tcagatacga tgatggttgc cacagtcagt cctgaagaca
    12961 aacagacaac gattgtcagc attcctcgtg atacgtatgt cgacatcgtt ggacatgaca
    13021 caaaagacaa gatcaaccac gcttacgcat tcggcggagc tgccatggcg atggataccg
    13081 tacaaaaata tgtagacatc ccaatcgatc actatgtttc aatcaatatg aaaggtctaa
    13141 aagaactcgt tgatgcagtt ggtggtatcg aagtcaataa tgacctcacc ttctcacaag
    13201 atggctatga ttttacgatc ggtaaaatca cattgaacgg ggatcaggca ttgtcttatt
    13261 ctcgtatgcg ttatgaagat ccaaatggtg actacggcag acaagaacgt caaagaaaga
    13321 tcgtagaagg tgtagcgaaa aaagtgttga gtctgaacgg tgtaagcaac tatcaatccg
    13381 ttttgaatgc tctagagtcg aatatgaaaa cagacatgag ctttgatgaa atgaaaaaga
    13441 ttgctttaga ttatcgggat gcatttggta cgatcaagca agatcagctg caaggagaag
    13501 gcttcatgca agacaatatc tcataccaaa aagtcagcga tcaagaactt gatcgtgtga
    13561 aaaaagaatt aaaagatcaa atgaatctag agaataaata ggaaagtgga gaaaaaatgg
    13621 aagagacaat cagcttgaaa gaaatttttg aaacattacg caaacacgtg ataacaatcc
    13681 tcatctcaat gtttgctggc ttggccatct caggcatcgt gacgtttttc atcttgacgc
    13741 caaaatacag ctcacaagcc caattgatcg tgacactgcc gcaatcagat actgcaaatg
    13801 tcaatgatgt caatacgaac ctgcaaatga tcgatacgta taaggatatg atcgtcagtg
    13861 atctagtact aaatgaagtc aaagatcgtt tagaaacaga agacaatgta aaaatgactg
    13921 ctggacaaat caaagatgcc atctccgtca atcagtcaca aaattcacaa atgttctcga
    13981 tccaagcaac aagcacaaat gcagtgacgg ctcaacaaat cgctaataca acgtcacaag
    14041 tattccaaga gaacgccaaa gacgtgatga atgtcgataa aatctcgatc atctcaggtg
    14101 cagttgccaa tacgacacct gtttcgccaa ataataaatt gaatctagtg attggcttag
    14161 tgttaggaat gatggtaggc gtaggtctag ctttcttatt tgaattactc gaccggacag
    14221 taaaagatga aaaatatgtg acggatacat tgggcttccc gatcttaggg actgtaccag
    14281 aaatgtcagc gaaagaattg aatgctggta tccgtaaaaa aggaccagct ccgacacaac
    14341 caaaagctac aaacgataaa acagcgccat cacgcagaag ccgctcaaga gtctaggagg
    14401 aaagaaaaat ggcacgaaca cagaaacaaa aagcagccca aactcatgca gtcagtttgg
    14461 tcacactcac aaacccctct tcaccaatcg ctgaacaata tcgtacgatt cggacgaata
    14521 tccagtttgc ctccgcagct ggtcagcaaa ttaaaacgat tgttgtgact tcatcaggac
    14581 ctggagaagg gaaatcgacc actgcagcaa acattgccgt tgtttttgcc aaatctggtc
    14641 aacgggtgct gctagtcgat gcagacttac gtaagcccgt catctataaa acgttcaagt
    14701 tgaacaacgc aagcggatta agtaccgttc ttagttcatc aggaagtgta gcagatgaga
    14761 tccaacgaac accagtcgag aatttgtcga ttctgccaag tggacctaaa ccaccaaatc
    14821 catcagaact actaagttcg ccaagaatgg atcagatttt agcagaagca agacaactat
    14881 ttgatgtagt gatcttcgac atgccgccag tagttgccgt aacagatgca cagatcatgt
    14941 cttccaaaac ggatggcacc ttattggtcg tacgtgaaaa tatgtcacgg aaagaatcat
    15001 tgactaaagc aaaagaattg ctggatatgg tacaagcgcg agtccttggt gttgtataca
    15061 atggggcaga acacagcaaa gatgctggct attactacgg gaattaagaa ataaaaagag
    15121 aggagacaag agaaatgatc gatctacatt gccatatcct gccaggaatc gatgacggag
    15181 cagaaaacat agatgccagt atcgccatgg ctgaaaaagc gattcaacaa gggatcacac
    15241 atattttatg cacaccccat cataataatg gaaaatacag caatgaaaaa tcacaagtca
    15301 tttctctagt cgcttcgtta caagcagaat tagagaaaag acaattgcct ttgacgttat
    15361 tggaaggaca agaagtacgt attaccggaa cactgatcga agacatcgac cgagatgaaa
    15421 ttttatttac agatttagat gatacatatc ttctaatcga atttccgaca ctggaagtgc
    15481 cgttatatgc agaacagctc ttttttgagt tgttacaact aggaaagaca ccagtgatcg
    15541 tccatccaga acgcaacgcg cattttcgta aagacccgaa tcatatgctg ccattcctag
    15601 atatgggatg cttggcccaa ctcaccgcac caagttatgt cggtgtattt ggaaaagaca
    15661 tccagaaaac agccaaaatg atggtcaaac ataatcttgt gcagatggct gcttctgatg
    15721 cccatggggt ctctaagcgc accttttact taaaagagtg ctataagcag attgccaagg
    15781 actttggtaa ggaaaaagta gtacagatga aacaagtcgc aaaggatctg atcaacgggg
    15841 atgaaatcag ttatccagtc tatgaagaga taaaaaagaa gaagtttggt ttattttagg
    15901 gggagtgtaa ggtgtttgta ttaacaagaa gaagaaaaat tgccacattg gttgttgtgg
    15961 acagtttgtt attgatcgct gcaaatattg cagcagctaa attcatgaag ccgtttgtag
    16021 ctataccaat ggaccttatt ttaatctcta ccggtttgtc gattggtttt tatttatttt
    16081 atggtagttt attcaaagtt ttcactcgga tcaatcgcta tacaaattta agagagatca
    16141 ttgcaatttt tggttcacta tctgcaagtg acgtctcaag tatcctcata ctgctgttca
    16201 ttaatcggca atacagttta cggttagtta tctttgctta tctgctttcg ctcttattga
    16261 tcatcggtag ccgattgatc tggcgtatct acatcgaaac aaaaaatatg agatatgtat
    16321 cagcggattc tgctaaaaat acattgatcg ttggtgctgg cgaaggcggg cgtattttat
    16381 acaacagctt tctgggatcc aaaacggctc aagatatcca agttgtcgga tttgtagatg
    16441 atgatccgaa taaaagaaat acgtatcttt caggaaaaaa agtattaggt gcattaaaag
    16501 atattccgga attgattgaa aaatatgaca ttcaaatggt aacgatcgct atcccttcct
    16561 tatcaagaaa aaaattacga agaatcttcg agttagtcga atccgctcac gtgaaagtca
    16621 acacgatgcc ttctatagaa gaacttgctt caggaaaaat tagcgtatca aaattaaaaa
    16681 caattgacgt agtggattta ctgggaagag atgaggtaaa gctggatatt gaatccatca
    16741 aagatcagat cacagacaaa gtcattcttg tcaccggcgc aggaggatcg atcggttcag
    16801 aaatctgtcg gcaaatcatt caattcaatc ctgcaaagct attgctttta ggacatgggg
    16861 agaactcgat ctacttgatt gaccgtgagt tacgcacgca tcatcaaaat tgtcctacag
    16921 aaatcgtacc gatcatcgca gatatccaag atcgcgagaa aatcaatgag atcatggaac
    16981 aatatcatcc agatatcgtc taccatgcag ctcctcataa acatgtacca ctgatggaat
    17041 ataatccaaa agaagcagtc aaaaacaata ttttcggtac aaaaaatgta gcagaagcag
    17101 ctaaagcagc aaaagtgaaa aacttcgtca tggtatcgac agataaagct aacaatcctc
    17161 caaatgtcat gggttcaaca aaacgaatcg ctgaaatgat cgtgactgga ttgaatgaag
    17221 aaggctgtac gaaattctca gctgtcagat ttgggaatgt ccttggttct cgcggtagtg
    17281 tgattccagt attcagagaa caaattgcta aaggtggtcc aatcactgta acagactttc
    17341 gtatgacccg atacttcatg acgattcctg aagctagtcg tttagttatt caatctggcg
    17401 cattagcaaa aggtggcgaa atttttattc tagatatgag tgaaccagtg aagattgtag
    17461 acctagccaa aaacatgatc cgattgagcg ggtatagcga agacgaaatc gaaatcattg
    17521 aaacaggtat ccgtccaggg gaaaaactat atgaagaact actgttggat aaggaaagaa
    17581 atgatgaagc agtttatgag aaaatctttg taggaaacat caaaggttac tcaatacaaa
    17641 aagtgatgga ttttgtcaag agcctgccgc aagacgatga acaattggct aaagatatcg
    17701 tcacttttgc aaatgcttcg aataaatagg ggggaaatat aaatgaataa attagaaaaa
    17761 cgtcagatac cattttcacc gccagatatt actgatttag aaataaaaaa cgttgtagaa
    17821 gtattaaaat ctggatggat aactacggga ccaaagacaa aaaaattaga aaaaaaaatt
    17881 acaaagtggt taggaacttc taaagtagtt tgtttaaatt cacaaactgc tgctgcagaa
    17941 acagggttac gtttgctggg aataggagaa aatgatgagg ttatcgtccc agcttatact
    18001 tattctgcca gtgcatcggt tgtatgccac ataggcgcga acctagtatt aatagactgt
    18061 caacaagatt ctttggagat ggattacgat gcattagaag cagcaattac ggagaagaca
    18121 aaagtgatta ttcctgttga tcttggtggt ataccttgtg actacgaccg tatttttgag
    18181 attgtggaga aaaaaagata tttatttcaa cctagtaatg agattcagaa agcttatggt
    18241 cgtataattg ttatggcaga ttctgctcat gcatttggag ctaattggca tgatcagatg
    18301 gttggtaatg ttgctgactt gacaacattt tcatttcatg ctgttaagaa ttttacaact
    18361 gcagaaggtg gagctttagt atggaaagag cacgacaaac tagataatga ggcaatgtat
    18421 catcagtgcc agctgctttc tttgcatgga cagtcaaaag atgcacttac taaaacacag
    18481 ttgggtgcat gggaatatga tattgtgggt ccatggtata aatgtaatat gactgatgtt
    18541 actgcatcaa ttgggttggc acaatttgaa cgctatccta atattctgaa aagaagaaag
    18601 aatataatta agaggtatga tgaagccttt caacaactag gaattgaagt tcttatgcat
    18661 tatacggatg attatagttc ttcaggccat ttatatatta ctagagtacc taacattagt
    18721 tctgagcaaa gaaatgaaat aattataaaa atggcagaac aagggattgc ttgcaacgtt
    18781 cactataaac cacttccaat gtttacagca tataaaaata tggggtttga tattaataac
    18841 tatcctaatg cctatgctca ttttgtaaat gagattacat taccattgca cacgaaactt
    18901 acagatgaag aagtagaata cgtaattaat aactatagta agattgtaaa ggatttggtt
    18961 ggatgatagg agaggcaaaa tgagaaagtg ggaagatttg ccaaaagaaa tgcagaatga
    19021 tgaaatacgg tattattatg atatcctcag taaaaaaaaa ttcagcttag tggtaaaaag
    19081 agtatttgat attttagtgt ctgcaacttt gttactgaca ctttcatggt tattttttat
    19141 tcttgcaata gtgataaaaa ttgattctcc tggacctgta ttttatagac aagaacgtat
    19201 tactcgttat ggaaaaaaat ttaaaattta taagtttcgt actatggtaa taaatgcaga
    19261 taaagatggc actcttgtta ctgtgaaaaa tgatgatcgt attacaagag taggaaaggt
    19321 aatccgtaaa tatagattag atgaaattag tcagctaatt aacgttttgg tcggagatat
    19381 gtcttttgta ggaaccagac cagaagttgg
//
LOCUS       DACDRK010000046        19053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDRK010000046 DACDRK010000000
VERSION     DACDRK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.046"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            215..1402
                     /locus_tag="IWJ77_001763"
     CDS             215..1402
                     /locus_tag="IWJ77_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9453364.1"
                     /translation="MQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKK
                     QVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIES
                     VTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWEN
                     TDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFE
                     SHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWS
                     SQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFD
                     FQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAV
                     QNK"
     gene            complement(1494..1859)
                     /locus_tag="IWJ77_001764"
     CDS             complement(1494..1859)
                     /locus_tag="IWJ77_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9453365.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1856..2221)
                     /locus_tag="IWJ77_001765"
     CDS             complement(1856..2221)
                     /locus_tag="IWJ77_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9453366.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2413..2661)
                     /locus_tag="IWJ77_001766"
     CDS             complement(2413..2661)
                     /locus_tag="IWJ77_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453367.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2660..2788
                     /locus_tag="IWJ77_001767"
     CDS             2660..2788
                     /locus_tag="IWJ77_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9453368.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2845..4539
                     /locus_tag="IWJ77_001768"
     CDS             2845..4539
                     /locus_tag="IWJ77_001768"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9453369.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4639..4812
                     /locus_tag="IWJ77_001769"
     CDS             4639..4812
                     /locus_tag="IWJ77_001769"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453370.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5190..6323
                     /locus_tag="IWJ77_001770"
     CDS             5190..6323
                     /locus_tag="IWJ77_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9453371.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6354..6674
                     /locus_tag="IWJ77_001771"
     CDS             6354..6674
                     /locus_tag="IWJ77_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453372.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6703..7167)
                     /locus_tag="IWJ77_001772"
     CDS             complement(6703..7167)
                     /locus_tag="IWJ77_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453373.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7299..8552)
                     /locus_tag="IWJ77_001773"
     CDS             complement(7299..8552)
                     /locus_tag="IWJ77_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9453374.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8581..8898)
                     /locus_tag="IWJ77_001774"
     CDS             complement(8581..8898)
                     /locus_tag="IWJ77_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9453375.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8924..9244)
                     /locus_tag="IWJ77_001775"
     CDS             complement(8924..9244)
                     /locus_tag="IWJ77_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9453376.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9708..11123)
                     /locus_tag="IWJ77_001776"
     CDS             complement(9708..11123)
                     /locus_tag="IWJ77_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9453377.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11140..12456)
                     /locus_tag="IWJ77_001777"
     CDS             complement(11140..12456)
                     /locus_tag="IWJ77_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9453378.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12606..13304)
                     /locus_tag="IWJ77_001778"
     CDS             complement(12606..13304)
                     /locus_tag="IWJ77_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9453379.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13593..14582)
                     /locus_tag="IWJ77_001779"
     CDS             complement(13593..14582)
                     /locus_tag="IWJ77_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9453380.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14662..15495)
                     /gene="msrA"
                     /locus_tag="IWJ77_001780"
     CDS             complement(14662..15495)
                     /gene="msrA"
                     /locus_tag="IWJ77_001780"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9453381.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15670..17304)
                     /locus_tag="IWJ77_001781"
     CDS             complement(15670..17304)
                     /locus_tag="IWJ77_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453382.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17291..18979)
                     /locus_tag="IWJ77_001782"
     CDS             complement(17291..18979)
                     /locus_tag="IWJ77_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453383.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT         6035 a         3735 c         3171 g         6112 t
ORIGIN      
        1 tattttaaga cagctgtttt tatgtgggca attctatgta aataaagaag tcaatctaga
       61 aaagctctcc aatctcctgc atgtttgcaa aaccacttta ctcaatgata tcaataacat
      121 aaaaaaagaa tttgaagaac agatcgctta tacacatcgg gaaaaggacc gttacacttt
      181 atatttttca gaacatatac ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt
      241 gtttttaaaa acttgtctgc tttatttgga agaagatgaa cctgattatc ttcaattaac
      301 tgaatgtgaa ttcatctctg tcagcaaagc ttattcatta aaaaaacaag tattagctta
      361 ctttaatgac tgcgggatcg aaatcgaccg ctactcccct cgattcactg aaatggaaag
      421 acgtctgctg cttcttaatg tgtcgtatcg tttaggcggt tttaattctt gggagctacc
      481 tgaatcgttt tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa atagtggcag
      541 attttatgat aaagaaaata aagaaatctt atcgattggt tttgctatca gtttcctacg
      601 ccagcaagtg tgtgctgtga caatagattc aaaatttatc gaagaaatca aaaaacgtcc
      661 tatttacaat tatgtcgaat cagcatggga aaacactgat ttccaaacct attataaaaa
      721 agaagaattc gcctttattt tgacactatt caatctttgt aattatggtt tccattctta
      781 tcagttgatt gcggaagact ttcaacagct acaccaagtg tttattgaca atactcccga
      841 aataaaagaa ctagttgcta cctttgaatc tcacttcaat caagaattat tcggaaacca
      901 accgttcgaa cgtgcgctga ttcatctgat gcgttcggct tgggataact atcaattatt
      961 catgccagaa aaattttatc ttttaaatga ggaacagaca aatttatata aagaagtcca
     1021 gacaattttt tccagctggt ccagccagtt accttatgat ctacggttga atcctaattg
     1081 catgcgggct tttgtgatcg aattgagcgg aattctccgt ctgacaaaag aacatctgac
     1141 tatctatatc gtcacaaatt cggatgttca ttatctgatt tatcgggaag ccttggaagc
     1201 tgtaactact ttcgattttc aagttgcacc aacaatttat tcaagtatat ctgatataaa
     1261 gaagtatgcg cagcagtcct caaaccgggt attatgtgaa cgtacattat atacaccaga
     1321 tgcggtccaa tatgaaaata ttattcctat ttcgatcaat acgatcgatc gagctatcat
     1381 ttcagctgtg caaaacaaat aaggggctgt gacaaaaatc atgtcacagc ctcttatttg
     1441 ttgaagatgc ccatttctgt aacaatctct tgatacacgt caggctcaca acctcactta
     1501 ttttggacat ctaggggtcc gatctcttta tttaaaaaat aagaaatgat ggttcggata
     1561 actaccaacg ccgctagttg gaaaatatcc tgcaaggtag gtttgataat cgattcgatg
     1621 atcccagctg aaacaagaat ttccaaactt agaagtatat agcctcccaa catcttttta
     1681 atagcattgt tctcgcttac tcggtcgctt tgcacttttg tatggacaaa taaatatttc
     1741 aaaaaatcct tgattgatag acaaacaccc caaaccaata aacagatagc aaagatatca
     1801 agtcccagta caagtttttc gaaatatgga agaagaaact gcatcaactc tgtcgtcatc
     1861 ctacctctcc tcttctttgt ttttctttgt cttctatttc ctgattcaag aaataagaaa
     1921 tgactgttcg aatgataata ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga
     1981 taactgactc gatgatatca gcaacgacta gtacttccaa gcttaacaaa atataacttg
     2041 ctagcagttt tcttgtatct gcattacgag tattacgttc tgcatagttt ttcttgacat
     2101 gagaccaatc aaaaagtttg aatagaacaa gaacgatccc gacaattaag ataccgatag
     2161 aacaaatatt taaaatagca atgatccatt ctagaaattc aattaaattt gagcgaaaca
     2221 tatacgctac ctactttctt ttaatttgtt cctattcttt tattgtaaaa gaaagttacc
     2281 ttgttcgtct atttaaaagt tgttctaatg ataacggttc gcttaaattt ttctttttca
     2341 ctctcccatt tttctccctc catccataaa tcgtggaagt atccaaaaca acgtagcaag
     2401 ttaaaataag gcttagtccg tcatcagctt ttcaaagaag cactgtctcg gtgctttttt
     2461 tgtacccaaa tttctaaaaa tagaaccata cacctcctca aatcttttct tcttacctac
     2521 cctattttta tttacaggaa aatcagacac ttcttctttt ttgtcaaaat ttcctttttc
     2581 ttctcgtcct atcatttggc attctaataa aaacgaacta tccttggata tagaaagaga
     2641 aaacaaggag gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt
     2701 gcttgccctt ggcgctgcat atctaggaaa aaagacagga ctattcgaag atgacagtca
     2761 tttatatgat gaattcgaat caatctagca tcctatttag ttgaatcact acggacatac
     2821 ttggaagaca ttggaggaat cacaatgaga aaaagtaaag caataatgat tggtgccggt
     2881 attgccaata tggcagcggc tgtttatttg attcaagaag gcaaatggaa aggggatcag
     2941 atcacctttt attcattgga tgatcacggt tcaaatgatg gtgcaccgac cgacactgta
     3001 gtggatgaat attggaataa aaaccatcca ttagaaaacc aaaaaggcta tattgctcga
     3061 ggcggtcgca tgcttaatta ccgtacctat gtagatttga tggatctgtt aagtcgaata
     3121 ccttctgtta ctgagccagg gatgactgct gaagaagata cgagagattt tgatgcaaaa
     3181 caccgaacct ttgacaaagc acgtttgatg gaaggtggca aaggaatcat tgatggcgga
     3241 aaactgggat tcaataataa agaccgtatt cttctgacta aactgattgc tatgcctgat
     3301 tcagaagaag aaaaactaga caatatcact atcgaagaat atttcaaaga tgatccacac
     3361 ttttttgaaa caaacttctg gtttatgtgg gaaacaacat ttgctttcag aacacgtagt
     3421 tctgctcaag aattgcgtcg ctatatgcat caaatgattt atgaattcac tcaaatcgaa
     3481 catctagtgg gcgtcaatcg aacacgttac aatcaatacg aaagtattat gcttcctttg
     3541 atcgagtact tgaaagaaca aggatgcaaa atcattttga accgccgtgt cactgactgg
     3601 gaattcaaag aaacaccgat gcaagatgaa atcactgtga ctggtttgaa aattaccaat
     3661 acactgactc aagaagaaga atacgttgtg gtcgatgaag atacagcagt tattttcact
     3721 aatgggtcca ttacagattc agctacttta ggagattacg aaacaccagc tgtagaaaat
     3781 atggattatg gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg
     3841 ggaaatcctg acaaattctt tgctgatcga gatgcaagcg aatgggtcag ctttaccctg
     3901 acgacgaaaa accatttgtt gttgaatgaa atcacccgta tcacgactca agaaccagga
     3961 aatgccttga attcatttat ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc
     4021 aatatgtcta ttgtcgtgca tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca
     4081 gttatttggg gttatttcct ttatccaaga cgacgtggcg aatttgtgga taaacagtat
     4141 attaaaatga atggaaaaga aatgctagaa gaactgattg gacaactttc aaaagtagat
     4201 cctggaccag tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt aataaataat
     4261 atccctgtct atatgcctta tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga
     4321 ccaaaagtca ttcctaaaca ttcgaccaat ctggccttta caggagaatt tgtcgagcag
     4381 ccttatcaga tgatctttac tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc
     4441 cattttgcgg gtattcctat gtcccgtctt gtcaaaacac cgcgttacga caaagatatt
     4501 cctactctag ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg tgaaaaagct
     4561 gccctgcatc aagtaataag aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg
     4621 ttggacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
     4681 gctgccctgc atcaagtaat aagagcagcc aagctaaaac agcttcttat gtttttgact
     4741 tggttagact tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttatgaa
     4801 aaaggcgttt agtttcgagt atgaagaaaa attttgtgaa aatggttcct cacgtttttg
     4861 cataaatttg acttaatacc gagaaactga tcccttctgt cataacctct ttttatttat
     4921 gtctgtttct ttttgaaaac aactattttt tcaacgttta ctctcttgcc aatccaacat
     4981 catctaacgg atacaactat ttcaatgatt accattacac taaaaaatcg gaaacacacc
     5041 tctccgaaca caaatacata agactataga atgtttcatt taagaaaaaa agcgtttcat
     5101 tatcaaaaag gtaagatctc ctttaaaatg aaattatctg tagtactaat aatgcagaca
     5161 attttatgaa tctgaaagga gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga
     5221 aaatggaaat cagaaacgta gatgatccaa aaatattaga accaacagat gcaatcatcc
     5281 gtatcacagc aacagccatt tgcggctccg atctgcatct ttaccatcat ggtgaagcag
     5341 ttttggaaaa agggtatgtt gtaggacacg aaccaatggg aatcgttgaa gaagtgggat
     5401 ctcaagttca aacattgaaa aaagggcaac gtgtggttat cccttttaat attagttgtg
     5461 gtcattgtca tttctgcctg aatcacatgg aaagccaatg tgatgaatca aatccagatc
     5521 agttatatgg cgggcttttc ggatttggca agttgaacgg taatcattgg ggtggtcaag
     5581 cggaatatct aagagttcct tatgcagact ctacttcatt tgttgtacca gataatgatc
     5641 ttcctgatga aaaagtttta tttctatcag atattttgcc cacagcttat tggagcgttg
     5701 aaaacgcagg agtaaaaaaa ggcgatacag ttgtgattct aggttctggt ccagtcggtc
     5761 tattcgctca aaaattcgct gttatggctg gagcagagcg cgtaatcgca gtagatccag
     5821 tacagcaccg tctagataaa gcggctaatt acaatggggt tgaaacttat ttcttagaag
     5881 acacggcaca agctggaaca gaattgtatg aactggctaa aggtggcgca gatgtcatta
     5941 tcgattgtgt aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa
     6001 gcttacaaag cggcacaatc tctccattac agatggcatc gcaagctgtc aaaaaatttg
     6061 gaacagtcca agtaactggt gtttatatga ctccagcttc ttcttacccg ctgcaagaat
     6121 tttttatgcg ggatatcgct gtcaagcatg gacaggcacc aattatccat ctaatgccaa
     6181 aaatttacgc aatgattgca gaaggactat tcgatcctac acaaatcatt actcattcgc
     6241 tgccactatc agaagcagca caagcatatg agatctttga caaaaaagaa gataaaaata
     6301 taaaagttgt tttgaaacct tgaattcagt aaaaaataca ggtgattttc tttatgccca
     6361 aaatcaaacg aaatacccat gagatcgaaa taggaaaaga agaagatatc gaaatacgag
     6421 gacgtcgctt ccgcttctat ttccaaaatc gttacacatt gatctcactg gctgtcgatt
     6481 tattaaccgg gattttctat atttttggaa gccttgcttc cttaactaaa atccccgatc
     6541 gatatggcac ctatttttat ttggctggtg ctattttctt gacgattcgt cctattctca
     6601 gaatcgtaag gaacattttt atttacgacg aaaaaaaagc aatgaagaga acaagcaaaa
     6661 aaaaagaggc atgagaattt ttcaaatctc atgcctcttt tttcacccta tcgtatcact
     6721 ttgatacaga cgagcggtcg tcccactttc ttccttgacc attgtcaaaa atgtccactc
     6781 gtatcgttca taaaacgtgg tatgatctgt aattaagtaa agtttttcgt acccaagttg
     6841 atgcatttct tttctgattt ccgctaatat ccgcttggct atcccctgct tacgcttttc
     6901 cggttcaaca aacagggcac aaagatttgg tgtcagatca gggcggttat ggaagtcgtt
     6961 ttcaatgacg cccgctcctc cgacgatggt ttctttttca tctaagacca cataccattg
     7021 aggaatactt tccttattag cgattccagt acgaatactt tcctcataca caacagcagg
     7081 aactccccat tttgatgaaa accaattcgc gctttgtttt actaattctg ggtgttctct
     7141 taatttgatc cagttatatg taaccaatgt ttaaacctcc gactaaaata aaacgtttac
     7201 aaaaattata caagtcttta tgaaaattag caatgacaac aatagtgatg taaaaaaaaa
     7261 aacggaaaac aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt
     7321 cttctttctt ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc
     7381 aaattgacag cggctagtac accacctgca atactttttg ttgccaatac accgccaata
     7441 attggtggag ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc
     7501 gcaaagtaag caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc
     7561 gggttcaaca cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc
     7621 gcaatcgaca agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata
     7681 gctaacagta aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa
     7741 ggtttgttca cgataaacgg tgcgctatgt ccagctgcga ttgcattaac gtttgcttca
     7801 atggctggcg cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca
     7861 aagaaccata agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca
     7921 agtcccataa atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg
     7981 aatgcagcaa aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt
     8041 aatgaagcaa atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat
     8101 ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca
     8161 ataaacaaac cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa
     8221 gaaatcacac ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc
     8281 aagaaagcaa tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat
     8341 cctttccatg attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt
     8401 aagttattga tcattactgc taatgctcct aagatcatta acggcatact caccataaat
     8461 gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa
     8521 tgtttctcca taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga
     8581 ttacgcgttc aatcgacgat acagattaat gatctctttt gctaaatctg taaaagctat
     8641 agatgtcatc agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc
     8701 ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc
     8761 tgcatctttg attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc
     8821 ttccattgca tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag
     8881 atttttttga tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt
     8941 tttctgcttg gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta
     9001 catctaaagg aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta
     9061 cttgcggtcc taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg
     9121 aagcagatac agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt
     9181 ttgtgactag gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt
     9241 ccattttaaa cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac
     9301 tttaaaagcg ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac
     9361 acactaaaaa agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt
     9421 taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt
     9481 attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt
     9541 tttactttgc tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac
     9601 ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta
     9661 tttcaaataa ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt
     9721 tcgtcaggca aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt
     9781 ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta
     9841 aatgcattca ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt
     9901 gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg
     9961 taatcatctt gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata
    10021 ccgttttctg ttatcagcca agggatatta tgataatttt cttttagata catggctata
    10081 tcatacatga ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt
    10141 aaatcaaaga cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa
    10201 cgaggggcgc gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga
    10261 tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac
    10321 aactccagga actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta
    10381 taaaacaaat catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag
    10441 accatttcag gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc
    10501 tctactgctt tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg
    10561 ttttgttcat gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt
    10621 tcgttgaatg taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca
    10681 tacttagcat aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga
    10741 aaagctggga cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa
    10801 gagtcgatca tttcacttat atgctgagca tattcttcat ctactataca gttctcataa
    10861 tcagtaaaaa aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc
    10921 atcagatcca catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac
    10981 ccattatgcc aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct
    11041 tctttttttc ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa
    11101 ttttttggaa tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac
    11161 acgatttgct acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag
    11221 ttgcgtaata actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg
    11281 cattggccaa gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata
    11341 accaatcgtt ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag
    11401 catgatcggc ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga
    11461 taacttggtg atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa
    11521 accgatcgtg actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat
    11581 aatgttcggt acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa
    11641 aagcaacggt tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa
    11701 gacttgcgca ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa
    11761 tgggcgttgt acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat
    11821 gataaatccc gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa
    11881 actagcagaa acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt
    11941 cctcaaccag caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc
    12001 tttcgtatct gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt
    12061 cactaattca taaacagttg gattaacaga taaataagac atcactgcga tgataccagg
    12121 gaatacacca tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata
    12181 gatggtcaag aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc
    12241 taaaaaacgc aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga
    12301 aaacaatgtg ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt
    12361 tatcaccatg atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc
    12421 taatttatcc ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca
    12481 tctctttttg tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct
    12541 tttttaccaa atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg
    12601 aactgtcaat tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc
    12661 atatggacag tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg
    12721 atctctgtat gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta
    12781 aacaaagcaa ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta
    12841 gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct
    12901 cgaaaagaag tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt
    12961 gcatattccg ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag
    13021 tctcgtatca tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc
    13081 cctcgcttat cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa
    13141 gctagagccc gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat
    13201 tcatattcat tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg
    13261 atcttgtcag tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt
    13321 tcatctgatg tattttttcg taattattat aacacgtttg aatcatctga tgattttttg
    13381 tatacaaaat tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa
    13441 aagactagga taaaattcac tcctagcctt cgatcatttt tattttgaac gacaaaaaat
    13501 atgatgttca ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta
    13561 atatatggat aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat
    13621 tcttcttctg gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt
    13681 aaccgacttg ctccgaccag tacacttgta acagattttt gtcgcaaatt ccatgcaagg
    13741 gccatttgcg ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt
    13801 tccagagttg atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta
    13861 gaatcttcag gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg
    13921 aacgtgattg cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt
    13981 gcacgagaaa acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt
    14041 aaaatttcag tcattttttt tgtatcttct ccattatagt tggatatacc aatatataat
    14101 gcttttcctt gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa aggtgtatca
    14161 gggtctgggc gatgatgata gaagatgtca acataatcta gccccattct ttggagactt
    14221 tggtcacaac ttgaaatgat ggatttacga gagccccatt ccccatatgg cccttcccac
    14281 atatgataac ctgctttgct agaaataatc agttcatcac ggtaggcttg aaaatcttct
    14341 ctaaaaatac gcccaaagtt tttctctgca gctccagcag ggcctccata attattcgca
    14401 aggtcgaaat gggtaattcc catgtcgaat gcgcctctta aaatctctct ttgattatgg
    14461 ataggatcat aatcaccaaa attttgccat aatccgagag ataaaatcgg taatttcaaa
    14521 cctgaatgac cggatttacg atattccatt ttttcataac gtgcactatc tgcaatatac
    14581 aatttttctc ctcctcatca aatcttttta aaacgctttc ttttttctat ttttagttta
    14641 ctacatttta aagaaaagga gttaatcaaa atgatttttt cgtatatttt tttgccattt
    14701 gttttttact cgttgataga tcagaaattg attacgtgct tgctggattt ttttataacg
    14761 tttcggttgt tttttgtaaa attgctgatg atagttttct gctggccaaa acgtaccggc
    14821 tggttcgatt tttgtgacga ttggttggtg gaaagttcct gaactgtcta gtttctgttt
    14881 tgattgttcc gctaattctt tttgtcgttc gacttgataa aaaatcactg gacgatattg
    14941 cttccctcta tcttgaaatt gtccaaatgt atctgtggga tcagtaattt gccaatagat
    15001 tgtcagcaac tcgctataac ttatttctct tgtgtcataa atgatctcca cagcttccac
    15061 atgacctgtg taaccaccac ttacttgatc ataatttggt ttttcaacat gtccgcccgt
    15121 gtatcctgac aaaacagata ggatcccttt tttcgattca aacggctcga ccatacacca
    15181 gaaacatccc cctgcaaata ctgcacgttc ttgatagtca gcatcttgca tggcatgctt
    15241 ggaaaagtca tatttttgct ccttatcaaa tccttccgta atcaataaat aaaagtccat
    15301 tacatcaggc gtcagattca ttcggatagc taatggacgt agtgttgctt ctagttttga
    15361 taactgttct ttaacggata tattttctct tatgtcattc tttgtctgaa tcaatacttt
    15421 tcgttcccaa tctcgtgtat tcggattcaa aatcagatta taaagtgttt gaatgaatat
    15481 ttgttcctca tccatagatt ctcctccttt ccgaagaaga caagctagtg actttagctg
    15541 taatttatca taaagtgagg aaataagcga ttacttcatc cccctacaga tttttcgtat
    15601 aaaaaagaga gaatccttat cgacagattc tcctttttaa tcaatttatt ttataaatga
    15661 aaagttaatt taccttttat ggaaaactat tttaaaaaat aaacataaat atgcagttaa
    15721 tgtaacaaga taagaaataa tagtcgttac tgtataactt gtgcttactt taatatcagc
    15781 aactcctttg actccgctta tttgtaccaa tccattagca ttcacctgct cctttacagg
    15841 ttgatgattt atttctacat tcgttcctaa ataagcatat acaggaagtg tgattacttg
    15901 gctcttctca ttagttactc tatatatgat agaactacca gttgttttat aaggaaggtt
    15961 tacccattga ctgccaatct ttgcttcgtg ttttattata tgctccttat tctccaatga
    16021 agcgatagga gaataatcat ataaaaaata atccattcct ttagtggtat acgcctctac
    16081 tgtttctttg tttacaacaa tatgtcccgg ctgagaaatt attttttgat tgaaattttt
    16141 tttactagaa acagttaaag tgattaataa caaaacagtc ataaataaat aaaatagttc
    16201 tcttgtctta ttgtatcttc tatttttgaa aaatatcatc gttaatatca gagaagagaa
    16261 tattatttgc aagcttaaga tccgccaagg aaattgtaac aatttggctg gcgtattttg
    16321 cagaagatac catggcacca aactagttgt agctagccaa gtcaaacacg taccataata
    16381 aatctttttt tctatgtttg ttaatttttt aaataaaatc aggggaatac ataaagataa
    16441 cagaccaact agtcccattg agtaagtagt taagtcgttg ttcatagctt cagtcagact
    16501 atcgaacaca ttagatgcat gagcatataa atttgtcacg ctaggtttgt tgattttttg
    16561 gtagagcatt tgttcaaaca tcggatacca aaaagcactc gtcagaacca atgtcattcc
    16621 tgccgcacta aataattttt taaatatagc aataaaatct gcattcttga aacctttaga
    16681 aaccaaacga ataaaaataa aaatgataat taatgctgta ctcattaatg ttgatagtac
    16741 atggctgtac actagcaaac tcatactcaa tgctagcttt acagacggat acttttcttc
    16801 ttttccagag atgatttgat aagcataaaa taatacaggg ataacaagtg ttattgcgat
    16861 agcttcggca attgcggaac gatagtagat gtctactgtc cgataagcag agaaaatata
    16921 gagacaacta aataaaaatg ccgctttttt ttgttttaaa aaccgctcgc cataatgata
    16981 agctacgagt gttgtacaca agttcaataa ccaaacgtaa ataatatagc ttacaatcaa
    17041 attttttgtc atccaatata acattacagc tggaaaaaaa gtcagaaagg gataaaaata
    17101 atttacacca tatccagtat aattgaatgt ctcataatta atcggactgg taaaaatagt
    17161 cgataacccc ttgattcgag ataagtgaaa agaaagatct ccagtggtat aaatactctg
    17221 tgtatttttg aaaaagatga aagaatatat actactaact ataattaatc cactcaatat
    17281 tactactttt ttatttattt tcattcttct tgtttatctc ctttttagtc acatatcgag
    17341 aatctttcaa cttattaggt gcaaatcgtt taaaacttga atacaccact aaacaataag
    17401 tcagaatact gatccataaa gccatgagag cagttggagt atattttgtt gtaatttcta
    17461 tcttattttc tccagcattt acatccacat tcactaagcc atgtttttta taagagtcat
    17521 ttacgacttt accgtttact tttacctgtg tacctaaata agcatatact ggtactgtca
    17581 ctttttgctt tttgttgctt gccactgtgt aagtaatgtt attatctgca gctttatact
    17641 tccctttttc ccacgattct cctactttta cttcatgttt ttctaagtga ggcctttctt
    17701 ttatcgcttc tgttggggcg taatcgtata atcctcccat atctgatgtt gtataacgtg
    17761 ggagtgtttc ttcattgata acaatatgac cgtgttttga ttggataact tttgcgtaat
    17821 tttcttttgc tgctatagac gtgacgagta gaaacaccac agtagcgcca atgattaacc
    17881 atgaatattt tttattcata gtctttttca taaaaacaag cgttatgatc attgaagaaa
    17941 aaaagatttg gatacctaag atacgccaag gaaactggag taagcttatc ggtgtttttt
    18001 gaaacaaaaa ccatggaaat aaattagtta cgcataccca tgtagaaact acgccaacat
    18061 agatgatttt ttcttttttc tccatttctt tcagtatgaa taaaggaaaa atcaacgcaa
    18121 ccaatcctaa taaccccata gtgtatgttg taatatcgtt atttaacgct tgaatgatac
    18181 tatctgaaat agaaagtgct tctagctgca aatctgtttt gaagggactg tgtatttctt
    18241 gataattcat ctgctgcaac ataggatacc aaaaatagct agttaatgca caagtcaaaa
    18301 aaacagcctt tactaaattc acgaccacaa attttatatc gttttttatt tgcttgctta
    18361 aaaacaaacg aactataatc atacaagcaa tcaatgcact acacaatagt gtagttaaga
    18421 catgagtata cacaagtaga cttatactga cagataaagc gatgactttt ttttctctat
    18481 tgaatatcaa tccataagca taatataaaa cagctgggat aatcgtaaga gcgattgctt
    18541 ctgcaattgc ggatcgataa tagatatcaa ctgttcgata agttgcaaaa gtatataaac
    18601 aactaaataa aaaagcagca tctatctttt taaaaaactt ttctccataa taaaaactaa
    18661 tcagaatcgt acaaatattt aataaccaaa tatatagaac ataacttaat attaagttat
    18721 ttgaaattcc ataaaaaacg actgcaggaa ttattgtaag aaaaggataa aaatagttca
    18781 gtccatctcc ataattgtta aatgttgtat agttgattgg cccttcaaaa atagaagata
    18841 accctttgat tcgtgaaaga tgaaaagata aatcacctgt tgtataaatt tcttgagcat
    18901 ttctcataaa atttactgaa tataaagtac taagtaagat aagaactact aaaagaaatg
    18961 cttttttact tattttcata gatttgttgt tctccttttt taattctatt ttcagtatac
    19021 tagaattgtc tttttttaat aagttttgcg tta
//
LOCUS       DACDRK010000047        18737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDRK010000047 DACDRK010000000
VERSION     DACDRK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.047"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            256..531
                     /locus_tag="IWJ77_001783"
     CDS             256..531
                     /locus_tag="IWJ77_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453384.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="IWJ77_001784"
     CDS             544..2616
                     /locus_tag="IWJ77_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9453385.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="IWJ77_001785"
     CDS             2781..4667
                     /locus_tag="IWJ77_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9453386.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IWJ77_001786"
     CDS             4679..5626
                     /locus_tag="IWJ77_001786"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9453387.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IWJ77_001787"
     CDS             5646..6173
                     /locus_tag="IWJ77_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9453388.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IWJ77_001788"
     CDS             complement(6236..6889)
                     /locus_tag="IWJ77_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9453389.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IWJ77_001789"
     CDS             7021..7863
                     /locus_tag="IWJ77_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9453390.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IWJ77_001790"
     CDS             8021..8917
                     /locus_tag="IWJ77_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9453391.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IWJ77_001791"
     CDS             8920..9633
                     /locus_tag="IWJ77_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9453392.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IWJ77_001792"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IWJ77_001792"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9453393.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IWJ77_001793"
     CDS             10168..10395
                     /locus_tag="IWJ77_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9453394.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IWJ77_001794"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IWJ77_001794"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9453395.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IWJ77_001795"
     CDS             complement(11181..11723)
                     /locus_tag="IWJ77_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9453396.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IWJ77_001796"
     CDS             complement(11960..12358)
                     /locus_tag="IWJ77_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9453397.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IWJ77_001797"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IWJ77_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9453398.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IWJ77_001798"
     CDS             13471..14238
                     /locus_tag="IWJ77_001798"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9453399.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IWJ77_001799"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IWJ77_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9453400.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IWJ77_001800"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IWJ77_001800"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9453401.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IWJ77_001801"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IWJ77_001801"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9453402.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5089 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctctt
//
LOCUS       DACDRK010000048        17903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDRK010000048 DACDRK010000000
VERSION     DACDRK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.048"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..275
                     /locus_tag="IWJ77_001802"
     CDS             <1..275
                     /locus_tag="IWJ77_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HAP9453403.1"
                     /translation="ITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSA
                     RKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            475..789
                     /locus_tag="IWJ77_001803"
     CDS             475..789
                     /locus_tag="IWJ77_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9453404.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            810..1187
                     /locus_tag="IWJ77_001804"
     CDS             810..1187
                     /locus_tag="IWJ77_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9453405.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1197..1970
                     /locus_tag="IWJ77_001805"
     CDS             1197..1970
                     /locus_tag="IWJ77_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453406.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1992..3395
                     /locus_tag="IWJ77_001806"
     CDS             1992..3395
                     /locus_tag="IWJ77_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9453407.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3577..4761
                     /locus_tag="IWJ77_001807"
     CDS             3577..4761
                     /locus_tag="IWJ77_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9453408.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4758..5048
                     /locus_tag="IWJ77_001808"
     CDS             4758..5048
                     /locus_tag="IWJ77_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453409.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5045..5266
                     /locus_tag="IWJ77_001809"
     CDS             5045..5266
                     /locus_tag="IWJ77_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453410.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5308..6087
                     /locus_tag="IWJ77_001810"
     CDS             5308..6087
                     /locus_tag="IWJ77_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9453411.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6154..6648
                     /locus_tag="IWJ77_001811"
     CDS             6154..6648
                     /locus_tag="IWJ77_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9453412.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6704..7342
                     /locus_tag="IWJ77_001812"
     CDS             6704..7342
                     /locus_tag="IWJ77_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453413.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7414..7809
                     /locus_tag="IWJ77_001813"
     CDS             7414..7809
                     /locus_tag="IWJ77_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9453414.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7793..10246
                     /locus_tag="IWJ77_001814"
     CDS             7793..10246
                     /locus_tag="IWJ77_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9453415.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10243..12270
                     /locus_tag="IWJ77_001815"
     CDS             10243..12270
                     /locus_tag="IWJ77_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453416.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12267..13289
                     /locus_tag="IWJ77_001816"
     CDS             12267..13289
                     /locus_tag="IWJ77_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9453417.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13306..14235
                     /locus_tag="IWJ77_001817"
     CDS             13306..14235
                     /locus_tag="IWJ77_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9453418.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14480..14596
                     /locus_tag="IWJ77_001818"
     CDS             14480..14596
                     /locus_tag="IWJ77_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9453419.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14612..15415
                     /locus_tag="IWJ77_001819"
     CDS             14612..15415
                     /locus_tag="IWJ77_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9453420.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15590..15886
                     /locus_tag="IWJ77_001820"
     CDS             15590..15886
                     /locus_tag="IWJ77_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453421.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            15893..17845
                     /locus_tag="IWJ77_001821"
     CDS             15893..17845
                     /locus_tag="IWJ77_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9453422.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         6149 a         2959 c         3546 g         5249 t
ORIGIN      
        1 gtatcacact tactacagca cataaagtta aaggaaatga gtatgatact gttatcgcta
       61 ttaatcttct aaacgggaaa attcctcatt ggaatgaaat ttttaatgtt tctgataaag
      121 gaattagttc agcgagaaaa ctactatacg taatttgttc aagagcaaaa aataatttat
      181 ttcttttctc agaggtgggt tattttacga gtaaaggaga cttgctttat cccactcaat
      241 tattagataa aatagaattt gattataata tttaaaatac ctaggtagtc gttgttttaa
      301 atatagtagg aatcggacgc tcagtacgga gcgtctattt ttttacccaa ttttgaaagg
      361 aagtgaactt atgaaaataa aaaatcaaga atcaaaaggt cgctctcccc cctttaaaac
      421 catcacacaa ataagattca ttcaaacata aaaacagaaa ggataggtaa aaatatggaa
      481 cttaaatatg tcattcccaa tatggaaaaa acatttggac aattagaata tgctggtgaa
      541 gggaacattg aacaacgtcg aataaatggc agaccaacca ttctctctcg tagttacaat
      601 ttatactcaa cgattcaacg agcagatgat attgttgtca ttcttccctc cgaagctggc
      661 gagaaacatt ttgaatccga cgaaaaagtc aaactcatca acccacatat taccgcagaa
      721 ggttataaaa ttggcaatcg aggatttacc aattacatta tgcacgcaga cgatatggtg
      781 aaagcataag aaaggaaagg tgaaaaaata tgagattagc agaaggtatt gtgattgaaa
      841 aagaccagac atttggcaca ttgaaatttt ccgccttgcg tcgtgaggtt cgtatccaga
      901 atgaagatgg tacggtttcc gaagaaatta aagaacgtac ctatgactta aaatcaaaag
      961 gacaaggacg catgatacaa gtcagtatcc ccgcttctgt tcctttgaaa gaatttgact
     1021 acaacgcaga agtggaatta atcaatcctg tggcagatac cgtagcgacg gctacttttc
     1081 gaggggcaga cgttgattgg tacgtgaaag cagacgatct tgtattaaaa ggacagacaa
     1141 aacctcatca gaaatataaa aaagaagcgt ccgaaagtga aaaataggtg ttgcttatga
     1201 caaacacacc attgattttg aaagaaattc cgaaggatga agccatttct tttattcggc
     1261 aataccatta ttcaaagata ctccctagat tgtgtaaata ctttctaggg attttttcag
     1321 aagaaaaatt attaggcgtt gtggaattag gctggggtac gcaaccccta caaaccatac
     1381 gcaaactctt tcccgacagt tcccttcaaa ccacagatta tttagaaatt ggaaagatgt
     1441 gttttcttcc agaaatgaat caaaccaatt attttggcag tcaagctctt tcagctctaa
     1501 ttaagtggtt gaaagaacat acggactgtc attttttata tacactggca gatggaattg
     1561 agggaaaatg tggctatgtc tatcaagcgt ccaacttctt ttactgcggg tatttcaaga
     1621 caagtgtcta tcgtgataag cagtcatggg aaaagataca ccctcgaagt gctcgccttt
     1681 tattagagga aaatgctcgc tttgaacagg tagagaaaaa gcattggctt tcccaggcat
     1741 tttgcgaata caaagggatt gagaaaatca atggacgcat gtttcgctat ctctatccgt
     1801 tgacaaaaga agcgaaaaaa ctgttaggac acactctcta tcgacgtcat tattatccga
     1861 aagaaaaaaa tttacgcttt gaaaagcgaa tcgcttatca gaaatatgaa gccatttctc
     1921 aaccaacttt tgataaacag gctcgtatct acaatacaca attattttga taggtggaaa
     1981 ggaggatttt tatgccattg cttcatgaca gaggagagcg aatcaaagcc aacgataagc
     2041 acttggtcta tcattttgta ctaatttcat tgctggtagt ttttctagtc gtcctactcc
     2101 cttttcattg gagagcgtta caatcggtga actggcaagc agttcagtta gatacatttt
     2161 tctatcaact tcatacgcca tacttttgga gcagtattct cactgctctt tttgtatgcg
     2221 gacttatcag ctggctttat tatcactatc gtatagattc tataaaacag ttagagcacc
     2281 gtcaaaagtt agctcgtatg attttagaaa atcattggta tgaagccgaa gaaactcaat
     2341 cagagggatt ctttaaagat ttacccagca agaaaaccaa acaacgaatc cgccattttc
     2401 ctaaaatata ttatcgaatg aaagatggac taatccatat tcatgtagaa atcaccttag
     2461 ggaaatatca agatcagctt ttgcacctag aaaacaaact agaaacagga ttgtattgtg
     2521 aattagtgga aaaaacgttg aaagattctt atgtagagta tgtcctactt tatgatacta
     2581 ttggcaatcg aattggcatt gaagacgtgg tagctcaaga tggacgtgtc aaattgatgg
     2641 aatcggtcta ttgggagttc gattctctcc ctcacatgtt aattgctggg ggcactggta
     2701 gtggaaaatc ctattttatt ctcacattga ttgaatctct cctgcataca aatgcaaagc
     2761 tttatattct tgaccctaaa aatgctgatt tggcagactt aggggcggtc atgaataatg
     2821 tctattaccg taaagaagat atgttggcat gtatcaatca attttacgaa gatatgattg
     2881 atcgcagtga aaccatgaaa caacacccta actataaaac aggagaaaat tatgcttatc
     2941 ttggtttgtc tgccaatttt cttatttttg atgaatatgt ggcaatgatg gatatgctag
     3001 gtcgtgaaag taccaccgtt attcataaac tcaaacaaat tgtaatgtta ggacgacaag
     3061 caggcttctt tttaattctt gcctgtcaac gtccagacgc aaaatatttt agtgatggga
     3121 ttcgggacaa ctttaacttc cgtgtggctt tgggtcgtat gtctgaaatg ggctttggta
     3181 tgatgttcgg aagcgataca caaaaacaat ttttcttaaa acccatcaag ggaagaggtt
     3241 acgtggatac aggaaaaagt gtgattagtg agttttatac accccttgta ccaaaaaggt
     3301 acgatttctt aggagaaata ggaaaagtca ttcaaaaaaa gcaatctgag cctgtaccgc
     3361 acgaagtgaa aggtacaggt tcagacttgc tttgattggt gtggcgttag ccacgccaat
     3421 cctttagccc cctctatcta acaggggggc acaaatacaa ctgaaaacag gcaattctca
     3481 tcaaccaatg ttgatggtac aaaggattgc ggaaaatatg aaaggtgtaa actcaagggt
     3541 catagtttac atctttttta gattggaggt ttgcgaatgg aaaacgaaaa aatttggatt
     3601 aaggaattga aagaaaagcg actggcttat ggcgtttctc aaaataaact ggcggttgct
     3661 tctcatatta ccagacctta cctatcggat attgaaacag gcaaggcagt ccctactcaa
     3721 caggtgaaag aagatttact gaacgctttg gaacgcttca atcccgataa cccattggag
     3781 atgttattcg actatgtacg gattcgattc cctaccaatg atgtggctca aattatcggt
     3841 gaggttttac gtttgaatat ggattatatg ctacacgaag actttggcta ttattcttat
     3901 cccgaacact atcgtttcgg tgatattgtt gtcctagtct ctcatgatgt aagtaaaggt
     3961 gttttattag aactgaaagg aaaaggctgt cggcaatttg aaaactttct attggctcaa
     4021 catcggagct ggtatgattt ctttcaagat tgcatggaac ataaaggtat tttcaaacgc
     4081 ttggatttag ctatcaacga taaaacaggg attttgaata ttccagattt ggcaaagaaa
     4141 tgtaaacagg aagaatgcat ttccgtcttt cgaagcttta aaaattatcg cagtggtgaa
     4201 ttggttcacc gtgatgaaaa accatacatg ggaaacacac tttatattgg ctcattaaaa
     4261 agtgaagtct atttctgtat atatgagaaa gattatgagc aatacgtgaa aaatgatgtt
     4321 cccttagaag acgcagaagt aaagaatcgg tttgaaattc ggttaaaaaa tgaccgagcc
     4381 acccaagcca tgaaagattt attggcacat caacaggcgg aaaaaacagc ctttgaaatt
     4441 atcaatcgct atatccgctt tgcagataaa gacgacacta aacgtcggag tgattggaaa
     4501 actaatgaac gttgggaatg gtttatcggt aaaaatcgtg gagcattacg cctaaccaca
     4561 cagccagagc cttattcttt tgaacgaaca ttgaactggt tacatcatca agtcgcccct
     4621 acattaaaaa tcgcaagcat tttagatgtt ctcaacggta caaccattat ctccaccatg
     4681 attcaggaag ccaaactgac agaaaaacat gagaaattaa ttgaacaaca acatctagcc
     4741 atggaggatt tgattacatg agccttttaa caaaagaatt aaagaagtta gggtttcagt
     4801 gtggcattga gtttcaagcg tatatacaaa atacagggaa atatacgtct ctcattattg
     4861 agggaaaacg gcaagctggt gataccatct atacctatga tttttacaag gtgagatttt
     4921 ataataatta taccaatcga gtaaccgtct atggagagca tttaacccct tttcaattat
     4981 taaggcgtgt aaaaagctat atctattatc gagaaaaata tcttaaggaa aggaggacaa
     5041 tcacatgaat tttgggcaga atttgtacaa ctggttcttg gataatgcac agtcattggt
     5101 attgttagcg attgttgtca ttggactgtt tttaggtttt aaacgagaat tttcaaaact
     5161 cattggcttc ttgattattg ctctaatcgc cgtcggactt gtgttcaatg cagctggcgt
     5221 gaaagatgtg ttattaaact tatttaaccg aatcattggt gcttaacaga tatttttcat
     5281 gataaaatag gaagtatata ttttgttatg aaaggattta tgaatatgtt tacaaatgat
     5341 caatttaaat ctcttttaat taaaacatta gaaagaaaga gctcttcaaa ttattatgaa
     5401 ggaggtaacg agattgtttc taaaatgaaa atatcagagg taactttaga tgagactgac
     5461 gattttactt attataaagg atataaaaag ggctggaata cgctttgcac atatcttaaa
     5521 ataagagttc cttttgatga tttagacttt tttgaaagtc ataaagattt gattacccag
     5581 acagcaagct ccatatatga taaacaaggt gataatgtat tagtggatac tattttagta
     5641 ccacttccag agaattacga agtaattaac tttagtcagt tgaaaattag tgatgtggtt
     5701 tcacaagcta ttgaagacgc tgagtctttt atgagtaatg gtgaatatca acgtgccttt
     5761 gacagagttc acactgcttt tcatggatat ttaattgaaa tactaaaaaa atatgaaatt
     5821 acagttccta gagacgaaaa tctatctaaa ttatatagta ggatacaaca attaattgaa
     5881 aaagaaattc aacctactga acttgctgat atagtgaaga caacgataag gtcatcaaat
     5941 ggaatgatta gctctcttaa tgaagcaaga aataggcatt cacttgcaca ccctaataca
     6001 aatattattg gaaagagaga agcaaaacta ataataggta tttcaagtac agttacagat
     6061 tatattagcg gataccttga taagtagcgt cattaactga ttgctacttt ttttatgact
     6121 atttttataa aaaggatgtg ataagaattg gaaatgaaag tctatgtggc aaatttagga
     6181 cggtataatg aaggtgaatt agtcggagca tggtttactc caccaattga tgaggaagaa
     6241 atggcagaaa gaatcggttt aaatgaggac tacgaagaat atgcaataca tgattttgag
     6301 cttccttttg atgtggacga atacacgccg attagtgaaa tcaaccgtct atgtgaagcc
     6361 attcaagaaa tcgaaggtac acctatttat aatgaactca aagaaattca aggcatgtgg
     6421 tttagtagct tggaagaatt actggaaaac aaagaggata ttcattgtta tagtgattgt
     6481 gattcgatgg aagatgtcgc ccgttattac gtggaagaaa caggacagct tggtgaagtc
     6541 ccttccaatt tacaaaatta tattgactac caatctttgg gacgtgatat ggaaatagag
     6601 ggtaattacc tagttacctc tcatggggta tttgaatatt gtcagtaagt ttaacaatcc
     6661 gtgttataat taaattaata aatagcacgg agaggtataa attatggtaa atgtatttga
     6721 aaatcttaaa ctattaaaac tagacatgaa agataaaggc tgggtgatag attcctttta
     6781 ctttcgttac aaacagcaaa attatattgt acttgttaag ctatttgaaa aagaggaaaa
     6841 agttccagaa tatgctctgt taaaactaga atttttaaag gaaaacgatt tttcagatat
     6901 gctagctgtc tatgctaatt cggtaaaatt atatacagac acgaaaacaa taagagaata
     6961 ttttggtatc gaatacagta gtaacttggg agatgtttta tttcaattta gccaaactct
     7021 tgctcgattt attccgacag aagttagtga gaagaaaaat gaagaccaaa aagaagccat
     7081 gtgtttctca ttaagtcaaa gtgattcaga agatcccaga aaaaaatatt gtttttcagt
     7141 tagaagaaat cctttaaagg gaaatggtga gctgggaaaa cgaagtcctt ttaatgacaa
     7201 taaaacgaga ttgtatagac ccagcttata tgaacgatta gggtcagata ccaatttaag
     7261 tttcagatat tcaatgaatc cagatgatga agaaactgat gaaggtatta ttgcaaaatg
     7321 gacaaaaaat aaaattgaat agttaaagca ttgaaagagc aatcacctat tttgattgtt
     7381 ctttttctat ttttacggaa aggacgtgaa gacatgaaaa aaataagaag ttacaccagt
     7441 atttggtcag tagaaaaagt gatttatgcg attaatgatt tacaactccc tttcccaatt
     7501 acctttacgc aaatgacatg gtttatcgtt tctttgtttg tcgtcatact ctttggcagt
     7561 ttaccaccac tttctctgat tgacggagca ctactcaaat acttagggat tcccattgtt
     7621 ctcactgggt tcatgtgcca aaagaccttt gacggaaaga aaccctatgg atttctaaag
     7681 tctatcatta cctatgctct acgccctaaa gtaacctacg ctggcaaaca aatacgatta
     7741 aaaaaagaaa caatgaatga atcgattacg attgtaagga gtgaacaact agatggcata
     7801 tccgattaaa tatatggaag ataatctcgt atttaatcat gatggcgaag tattcgctta
     7861 ttatgaatta attccctata attacagttt tttaagttcc gatgaaaaaa ttcaagttca
     7921 tgataatttc tgtcaattga ttgcccaaaa tcgtgatggc aagattcatg ctctgcaact
     7981 agcaaccgaa tcaagtattc gagccacaca ggaacaatcg aaaaaagaaa tcactggacg
     8041 attaaaagag atagcttacg aaaaaataga tgaacagacc gaagcgttaa tttctatgat
     8101 tggtgacaat caagtagact atcgcttttt tattggtttt aaactctacc tcaatgaaca
     8161 ggaagtatcc gtaaaaaatg ttgggaaaga aattcaaaaa gcatttgcgg aattctttca
     8221 tggtgtgaat cacaaattaa tgggcgattt tgtttctatg cctatggcgg aaatcgaacg
     8281 cttttctaag atggaacagt tgttagaaag taaattatcc agacgctttc agattcgtaa
     8341 attggagaaa gatgattttg gttatattat tgaacatctt tacgggcaaa caggtgtccc
     8401 ttttgatgac tacgactacc atttaccgaa acgatacgaa gataaaaaag tgttggtaaa
     8461 gaaatacgat attctcaaac ccactcgctg tttaatcgaa gaaaatcaac gctatttacg
     8521 gattgaacag gaagatacca ctacttatgt ggcgtatttt acaattaata gtattgttgg
     8581 tgaattggat ttccctaata gtgaaatttt ttattaccag caacagcagt ttactttccc
     8641 cgttgatacc agtatgaatg ttgaaattgt cgccaataaa aaagccttat ctaccgttcg
     8701 caataaaaag aaagaactga aagatttaga tagccacgca tgggaaagcg acaacgaaac
     8761 cagtactaat gtggtggacg ctttagaatc tgtcgatgaa ttagaaagta atttagacca
     8821 aaccaaagaa tctatgtaca aactaagcta tgtggtacgg gtatcagcaa agaatttgga
     8881 cgagctgaaa caacgttgtg atgaagtaaa agatttttat gatgatctgt ctatcaaatt
     8941 agtacgacct tttggcgata tgctaggctt gcataatgaa tttattccct caagcaagcg
     9001 atatatcaat gactatattc aatatgtcac gtcggatttt ttggctggtc ttggtttcgg
     9061 tgctacacaa atgattggtg aaaattctgg tatttacgtc ggttacaatc tcgatacagg
     9121 cagaaatatt tatctccaac ctagccttgc aagtcaagga attaaaggct ctgtaacaaa
     9181 tgcattagcg tccgctttta ttggttcatt aggtggcgga aaatcattct ctaacaatat
     9241 gctggtctat tattcagtac tctttggcgg acaggcaacc attgttgacc caaaagctga
     9301 acgtggtgaa tggaaagaaa ccttacctga tattgcccat gaaatcaata ttgttaatct
     9361 cacgagtgag aacgacaata aagggttact tgacccttat gtgattttaa aactcccgaa
     9421 agattcagaa agccttgcca ttgatattct cacgttctta acaggtattt ccagccgaga
     9481 tagcgagaag tttcctgtcc tgcgtaaagc tattcggagt gtaactcaaa gcaaacaacg
     9541 agggttgtta ttggtgattg atgaattgcg aaaagaagat acgcccatca gcaatagtat
     9601 tgctgaccat atcgaaagct ttgtcgatta tgattttgcc catctgttat tcagtgatgg
     9661 cagtgtcacc caatccatta gcttggaaaa acaactcaat attattcaag tggcagattt
     9721 ggtcttaccc gattccaata gctcttttga agaatacact accatggaat tattaagtgt
     9781 ggcaatgctg attgtcatta gtacctttgc tttagacttt atccactctg accgttccat
     9841 ttttaaaatt gttgaccttg atgaggcttg gagtttttta caagtggcac aaggaaaagc
     9901 tctatctatg cgattggttc gagcaggacg tgccatgaat gcaggggtct atttcgttac
     9961 tcaaaacgcg gacgatttac tagatgaaaa gatgaagaat aatattggct tgaaatttgc
    10021 ctttcgttcc actgacctag tggaaatcaa aaagacctta gaattttttg gtgtggacaa
    10081 ggaagatgaa aacaatcaga aacgcttacg tgaattagaa aatggacaat gtttagtttc
    10141 agacatctat ggtcgtgtag gggttatgca gtttcacccg atttttgaag agttgcttca
    10201 tgcctttgat accagaccgc ctgtgcgaaa tgaggtggaa gaatgacaca aaaacaaaag
    10261 aaatttcttc actatagttg gataacgctc ctttgtgtgg gcgttatttt acttttatta
    10321 ggcacgctgg gcaatgcagt acaagcgaca ggcttagttg atgaaaccat tgatacaagc
    10381 aatgaatact ccaagtatgg tttgaatcaa tatcagcttg attattatgt ggacaatagc
    10441 tggggctggc ttccatggaa ttggagtgat ggcataggac aatcggtgat gtatggattg
    10501 tatgccatta ctaattttat ttggacaatt tctctttatc tatcgaacgc cacaggttat
    10561 ttggtgaaag aagcctatgc cttagatttc attagtcaaa catcccaagc aattggcgaa
    10621 aacatgcaga cgattgcagg cattacaccg aatggctttt ccagttcagg gttttatgtc
    10681 ggtttcctcc ttctttttat tctcattgtt ggaatctatg ttgcctatac aggattggtt
    10741 aagcgagaaa cgacgaaagt gatacgagct gttttaaact ttgtcgtggt gtttattcta
    10801 tcggcttcct ttattgcgta tgcacccgat tatattgcga aaatcaatga tttttccaaa
    10861 gatgtcagta ccgccagtct gaatattggg acaaaaattg ttcttcctaa cacggatagt
    10921 caagggaaag acagtgtgga catgatacgt gacagcttat tctctgttca agtaaagcaa
    10981 ccatggctat tgcttcaata tggctccact gatgtagaag cgttagggga agaacgtgtg
    11041 gaaagtttat tgtctaccag tccagataca aacaatggtg aagatagaga aaatgtagtg
    11101 attgaagaaa ttgaagatca tgacaatacc tatttaaccc taccgaagac gatttccaga
    11161 ttaggaacag tcttcttttt gtttgtattc aatattggta tctcgatttt tgttttcctt
    11221 ctaacaggaa tcatgatttt ttcgcaagtg ctatttatta ttttcgctat gtttttgcca
    11281 atcagtttcc tattaagtat gttgccgagt tttgatggca tgggcaggca tgccattacc
    11341 aagttattta atgtgattat gacaagggct ggtattacct tgattattac cgttgccttt
    11401 agtatttcca ctatgcttta ttcgctgtca gcaagttctc cctttttcat gattatgttt
    11461 ttacaaattg tgacctttgc agggatttat tttaaactgg gcgatttaat gagcctattc
    11521 actttacaaa gcaacgactc tcaaaacatg ggcagacaag tgtttcgcag acctcgtcaa
    11581 atgatgaatc gacaaaccag aagatttcaa cgcagtatga gtaagatttt tcgtggaaaa
    11641 gcagggtcaa cgaagcaaaa gacaatgaca acaaagcaga atcaaaccaa taaagctaac
    11701 catactcgtc aaaatgaacg gactacacca aagaaagaaa cagcactttc caatgtgaaa
    11761 aagcaacaag gcggtatcgg gcaacgatta ggtgaaaaga cagcccatgt attggacacg
    11821 aaaaatcgtc tagtcaataa aagaaagcag gcaaaacaac agatacaggc gacaccaacc
    11881 catgtcaaat atgcccttca tcaaggaaaa gagaatgtca ctcataatgt aacagacttt
    11941 aaacaatcta tggcagatac caaagctcaa cgcaaacaac agcgtatgaa ccagcaggag
    12001 caaaaacgaa agaacattgc tcaaaagcgt ttggagctgg ataaagcaaa agaaaaaaga
    12061 cgaaaagaaa acccatcatc tcctgttcag aataaccagc agcatgttcg caagcgtcag
    12121 caaaccgtca cgcctgtttt gtcacaaaac actaaacgtg aaaatcatca gcgaccattg
    12181 aaaaaagagc aaaaacaaaa gacaccaccg aagcaattca atcagaggaa gctaaataaa
    12241 agaacggata agcgaggaca acaaacatga agctaaaaat aacagtgatt tgtagtgtgg
    12301 tcttctctct cttctttttt ctcttattat tcctagtcat tttctttgca gatgatacgg
    12361 acagtggcga aaacaacaag gattcttcta ttccacaagg cggtgtgacc gtttcaccag
    12421 aagtgttggc acaccgtccg ttgattgaaa aatatggtaa ggaatatggg attgaagact
    12481 atgtttctta tatcttagcc attatgcaag tggaatcggg cggtacagca gaagatgtta
    12541 tgcaaagttc cgaatctttg ggtttaccgc ccaacagttt gagtaccgaa gagtccatca
    12601 aacaaggggt taaatatttc agtgaattat taaccagtgc agaacaacaa ggagtggata
    12661 tagattctgt tatccaatct tataattatg gcggtggctt tttaaattat gtgaggagtc
    12721 atgggaaaaa atatacctat gaattagccg aacagttttc taaagagaag tcgggtggtc
    12781 aaaaagcaga ctatcccaat cctatagcca tacctgtgaa tggtggttgg cgatataact
    12841 atggaaatca gttttatgtt cagttggttt cacaatacct aaccgacact tccccaacag
    12901 aatttgatga tgaaaccgtg caagtcatta tggacgaagc cttgaaatac gaagggtttc
    12961 cctatgtctt tggaggggcg tcgcctacca cctcttttga ttgtagtggc ttaatacaat
    13021 gggtgtatga caaagctggc atttctctcc ctcgtgtcgc ccaagatcag tatgacgcta
    13081 cgcaagaaat ctctatggaa gaagcccaag caggcgacct tatcttcttt cattcaacct
    13141 ataatgcagg cacatatgtg actcatgtgg cgatatattt agagggaaat cgcttttatc
    13201 atgcaggcga cccaatcggt tatggcgact taagcagtcg ttattggcaa gaccatctga
    13261 ttggggctag acgtgttatt cataactaag aaaggaatga atcaaatgat tataaaaatc
    13321 gagcgaaaag aaaagaagga gaaaccaaag aaatctaaaa aaccgagcaa gcaaagaaaa
    13381 gttccgatga ttaaagttgg cacgcataag aaattaacct ttgttttatg ggttttattg
    13441 attggtagtg tcggatttgg aatctataaa aattttactg ccattgacac tcatactgtc
    13501 cgtgaaacag aaatcattaa acagcagatt gtcgatacca accaagtaga aagttttgtg
    13561 aaatcctttg cgaaagacta cttttcgtgg caacaatccc aagaagccat tgataaacga
    13621 aatgaaaaat tgacccatta tttgacggaa gaattgcagg tattaaatga agaaatgatt
    13681 cgtaaggata ttcctactag ttctagtgtg aacgatatac aggtgtggca ggtttctcaa
    13741 gtgaatgaga acacctttga ggtactgttt tctgttgaac aagttattac cgaagacaaa
    13801 gacaaggaaa ctatatcgtc tagctttcat gtggttgtcc atatagatga atcagataat
    13861 atggttatta tcaaaaatcc gacaatgagt aagaaacctc aaaaatcgga ctaccaaccc
    13921 aaacagctag aaagtgacca tacagtagat acagaaacga tggacgaaat cactagcttt
    13981 ttagaaacgt tcttccagct ctatcctacc gccacagaaa aagaattgac ttactatgta
    14041 agtaatcatg tgttacctat gattaacaag gaatatgttt tcgaggaatt ggtgaatcct
    14101 atctttacca gaaaagataa ccaagtaatc gtgaatgtcg ctgtgaaata tttagatcaa
    14161 gaaacaaagg caacccaaat ctcgcaattt gagttgatat tagaaaaaca agataattgg
    14221 aagattgtaa aataacaaat attggtacat gattacagat actttgtaat catgtactct
    14281 ttttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagtg
    14341 actatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
    14401 atagcgggaa caaataatta gatgtccttt tttaggaggg cttagttttt tgtacccagt
    14461 ttaagaatac ctttatcatg tgattctaaa gtatccagag aatatctgta tgctttgtat
    14521 acctatggtt atgcataaaa atcccagtga taaaagtatt tatcactggg atttttatgc
    14581 ccttttgggt ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
    14641 tcatgttgat gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat
    14701 tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg
    14761 tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt
    14821 gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc
    14881 agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg
    14941 tatattattt catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat
    15001 tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc
    15061 cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac
    15121 cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat
    15181 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
    15241 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
    15301 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
    15361 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat attaagtagt
    15421 tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga
    15481 atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg
    15541 taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg
    15601 tgtgttaaaa tggttacaat ttactaagca aaaaggggtg cattttccta tgcaatttct
    15661 tgagccaaaa aataagaacg ctaagtctgt ggattgggaa atatcagagc aagtaagggt
    15721 gatagttaag caatacgctg aatatgcaga gcgtactgaa agcgaagcag tggatgaatt
    15781 cttattgaac attttagacg ataagaagtt tattgaatgg attgccaaca agcgaagtaa
    15841 taagcggatt gttgaaaaaa tgggtatcaa agatagagtg ggttgataga caatgaccaa
    15901 gcttaaaaga ttagccacga aggaagatga aattgtagat gttaaaattc cgatttcaaa
    15961 cgaagaatta aaagataggg ctaaacaata tgatttatta actccaaaac gatttgcaac
    16021 aaggtataac aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc
    16081 cattcaatat aattactgca tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa
    16141 attcaaagat gtaaaaggga aaccaagtcg atataaaatg actggtagtt ccaaagataa
    16201 aggtcataaa ggtatgtact gcaatgacaa tcctattggc agaggtgtat cgcaaaactg
    16261 tacagtaacc cctttatcta attggtctgt tgttgaagaa attaaacgcc ttatagaaat
    16321 caatagcatc caagatgttg agccagatta tcaatttcat aaagaaggct gctcggaaga
    16381 agaatcaacc ccattcaatg agccgaagca attttacaaa agggggaaaa gtaggggcaa
    16441 atctcaacgc tatcaatgta aggcgtgtaa gaagttcact aatgttctac caaaaagaga
    16501 agaaaccact acttatcacc aacaaaagaa caccattctt ccaatgttcg ctaagatggt
    16561 tgttggaagg gtttcagtca gtcgtacttg tgatatttta ggaattggag ttggaaccta
    16621 ctatcataag ctggagtggc tgtataggcg ttgtttggag tttttagaac gatatgagac
    16681 acaacccctt caaacaaaga aatttaacga gatgtggctt aatacggaca agatgcacta
    16741 ctacctaaat aacgtccgta agaaaggaca aggttcaaag aaatatacgg ggtttgaaga
    16801 ccttaatatg caaacgtata tagttgtatc tgctgaggtc ttatcaagat atgtgttccg
    16861 ttctgatgtt gcctatgatt ggaatatttc aatggatgaa ttaaatgaag atacaagaaa
    16921 attcaaagaa gaccatctca atacttttag tcgtaaaaat gataggttag attggtctta
    16981 ttatccacaa gaaccatccg ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt
    17041 agggaaaatt actaatagga gtagatttgt cgatggttta aatgttgatg ctccttatac
    17101 tacaactgct cactattggt taattaaaca aatggttaat gccgacgaat ggcgaatgat
    17161 tagtgatgat gacttctcta ttagaaacgc cttttataga gtattcacga aagaattaag
    17221 gttgagcgat gcacatcatt ttatttgcca agttaacaag acgaaaagca gaaaacaatg
    17281 cttaaaagaa ttcggacaag caaaagcaga actgctcgat tggggagata tcagaggatt
    17341 caaaacaaag tttctacgga ctttggcatc tcattatcta actgaattac taacaagtca
    17401 tcaattccac gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat atgccgataa
    17461 ccctatcaaa catccattag ccactaagga taaaggattt tattcagttg attgtagaac
    17521 ggatttatct gctctggaac caaacgaaat cgcaaagatg ctattaaatg ttaatgacca
    17581 ttcgaccaat agctttattc aacagataag acgttatata tcatctcttg aaagaccatt
    17641 aacaacagct cgtggtgata agaagagcta tatttacgct aacttcaatc cgaagtatgc
    17701 tcaatttgcg attacaattc taagaactta ctacaacttt tgtcgccctt ttaaatcagc
    17761 ggataaaaag gttttgacac ctgctcaaag attagggatt acagataaac aatttgattg
    17821 gaaagatata atttatttca aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc
    17881 tttatcttta ctgttcttct tca
//
LOCUS       DACDRK010000049        17891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDRK010000049 DACDRK010000000
VERSION     DACDRK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.049"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(48..1514)
                     /locus_tag="IWJ77_001822"
     CDS             complement(48..1514)
                     /locus_tag="IWJ77_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9453423.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="IWJ77_001823"
     CDS             1901..2497
                     /locus_tag="IWJ77_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9453424.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="IWJ77_001824"
     CDS             complement(2555..3037)
                     /locus_tag="IWJ77_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453425.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="IWJ77_001825"
     CDS             complement(3143..3340)
                     /locus_tag="IWJ77_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453426.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="IWJ77_001826"
     CDS             3635..4393
                     /locus_tag="IWJ77_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9453427.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="IWJ77_001827"
     CDS             4453..4881
                     /locus_tag="IWJ77_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453428.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="IWJ77_001828"
     tRNA            4927..4999
                     /locus_tag="IWJ77_001828"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="IWJ77_001829"
     CDS             complement(5149..5520)
                     /locus_tag="IWJ77_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9453429.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="IWJ77_001830"
     CDS             5735..5920
                     /locus_tag="IWJ77_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453430.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="IWJ77_001831"
     CDS             complement(6003..6191)
                     /locus_tag="IWJ77_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453431.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="IWJ77_001832"
     CDS             complement(6215..6478)
                     /locus_tag="IWJ77_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453432.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="IWJ77_001833"
     CDS             6636..6893
                     /locus_tag="IWJ77_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9453433.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="IWJ77_001834"
     CDS             complement(6964..7155)
                     /locus_tag="IWJ77_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453434.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="IWJ77_001835"
     CDS             complement(7296..7616)
                     /locus_tag="IWJ77_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453435.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="IWJ77_001836"
     CDS             complement(7639..8073)
                     /locus_tag="IWJ77_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9453436.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8408..9046
                     /locus_tag="IWJ77_001837"
     CDS             8408..9046
                     /locus_tag="IWJ77_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9453437.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9370..9453)
                     /locus_tag="IWJ77_001838"
     tRNA            complement(9370..9453)
                     /locus_tag="IWJ77_001838"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9417..9419),aa:Leu,seq:cag)
     gene            9981..10481
                     /locus_tag="IWJ77_001839"
     CDS             9981..10481
                     /locus_tag="IWJ77_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453438.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10671..11015)
                     /locus_tag="IWJ77_001840"
     CDS             complement(10671..11015)
                     /locus_tag="IWJ77_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9453439.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11169..12089)
                     /locus_tag="IWJ77_001841"
     CDS             complement(11169..12089)
                     /locus_tag="IWJ77_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9453440.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12276..12767
                     /locus_tag="IWJ77_001842"
     CDS             12276..12767
                     /locus_tag="IWJ77_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9453441.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12791..15166)
                     /locus_tag="IWJ77_001843"
     CDS             complement(12791..15166)
                     /locus_tag="IWJ77_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9453442.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15492..16118
                     /locus_tag="IWJ77_001844"
     CDS             15492..16118
                     /locus_tag="IWJ77_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9453443.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16141..16845
                     /locus_tag="IWJ77_001845"
     CDS             16141..16845
                     /locus_tag="IWJ77_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9453444.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17037..17870
                     /locus_tag="IWJ77_001846"
     CDS             17037..17870
                     /locus_tag="IWJ77_001846"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9453445.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         5555 a         3490 c         3015 g         5831 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acgtggattt gtcataaatt gtcatgcttc ttttacctat gaaatatttt
     8341 cacgtgttaa agtattcctc gttgaacaca acaaataaaa ctttttaacg aataaaggag
     8401 atttttcatg acatcactta aaactttact ttttggaaca actttggctg ccggcgctgc
     8461 attcttcatg ggaacgactg ctcatgcgga cgaagcttat accgttcaat caggtgatac
     8521 tctatcaacg atttctcaaa aatacgttgg tgataactcc ttgatcaatg ccattgcaga
     8581 atcaaattca atttctgata ttaacctgat ctactcagga caacaattaa caatcccaac
     8641 agaaggctct gcacaagcag ctgctgaacc tcaagcagcc gttcaagaag caccagtcca
     8701 agctgaacct gttcaagctg aacaacctgt tgtacaagaa acggttcaaa cggaaacaca
     8761 agcagcgcct gttgctgaaa cacaacctgc tcctgctgta acagaaactg ctgctactcc
     8821 agcaagtaca agctctgcaa aagaatggat cgcacaaaaa gaatcaagcg gttcttacac
     8881 agcaacgaac ggacgttaca tcggccgtta ccaattagat tcttcttact taaatggtga
     8941 ctactctgct gcaaaccaag aaagagtagc agaacaatat gttacttcac gttatggttc
     9001 ttgggaagcg gcgaaagcat tctgggaagc aaacggttgg tactaagatt cattatttga
     9061 acgtttaaac acattcaaaa tatagtaaga ggtcatgcga tttattcttt cgcatgacct
     9121 ctttttgagt ttaagtttag tctttattcc ttaactatcg tttgccagta tttcttgtat
     9181 cagaatctat aaatctacta tttgaatcaa tctattaggt tttaaactgt cccaccttca
     9241 cacaaatttt ttttgattta tttgaatctg gttccgttgt ttaaaatttt tcctatattc
     9301 ttttagataa tatgttattt tctttctcat cgctccaggt gtaccttctc tttataaaaa
     9361 aacaaattat gcggctaaga agactcgaac ttccacgagg tcaccctcac tagcgcctga
     9421 agctagcgcg tctgccaatt ccgccatagc cgcatcacat cttttagttt aggattacat
     9481 gtgttttttg tcaagaacat atttttgttc tttttcattt ctgtacgccc tcccttatgc
     9541 catctacaca aataattcat ccgagcaact ccatcatcta ttgagggttg gtgaaaaaat
     9601 ctgttttatc catatgatcg atttcaatgt taaacgaacg actctgggat tagtaactat
     9661 tttatttttt tcttcatttc ttttttcctc tttttctcga ttcagctatc ttctttcatt
     9721 tggatattga ttatttcttc tgttgattac tatccttctg gaattctaga cgctgaagta
     9781 attcacgttt tctttcttct agagatcata agtaacgaat gaacgaactt caaataattt
     9841 ttttattgta attcatattt gtattgttcg atgattctta atacgatatg acacaataaa
     9901 taaaaatagg catggctatt tacaacaata gtgctatagt agaaaacaaa tgaataaggt
     9961 tacttaaaga ggtgaatacg atgaaactga tcaaactatg ggaagcagat ttaaagaaag
    10021 cttatcagct acaaactagc tttggaccaa atgagagtgg atttatcaac aatgcttatg
    10081 gctatacatt tgctgaattt ttagattatg taaaaaaatg cgaaaaaacg tctttaggaa
    10141 tcgatcttcc tgagagttac gttcccgcaa ccgtctttat tttagaaagt gatgaacatg
    10201 agtatgtagg aatttttaat ttgcgccata aactgaacga aacattaaga aacggcgctg
    10261 gccatattgg ttacggcatc tcccctaatt atcgtaaaaa aggatatgcc acacaaggat
    10321 tagcattagt actacaagag gcaaaaaaaa tcggcattga tgaggcactt ttgtctgtaa
    10381 ataaaaaaaa tcttggcagt cttaaagcac aacaaaaaaa tggggctgcg atttatcgtg
    10441 aagatgagaa agaatactat acgaaaatca aactgatgta aaaaagaagg attccaaaag
    10501 gaaagggatt tgaatgtcct ttatcaatag aacacattta tttaattaac ttataatata
    10561 gcagacaaaa ttttataaac aaagaaacct tttatctctt ttcttctaga aaggataaaa
    10621 ggcttttttg tttttcctat ttgcttctca tagctagtca gccaagattt ctatcgtatc
    10681 cccaacgcaa tccgtgcata acggctcatc ttatctgtcg tccatgctgg ataccagact
    10741 agtttgactt ctgctttcgt tacttctggc acttctttta atgcgtcatg gatagattct
    10801 gttaaaatat ctgctaatgg acagcccata gttgttagag tcatcttaat gactgtttct
    10861 ccattttcag gattgaattc tatatcataa atcaagccta aattgacgat gtcgattcct
    10921 aattcaggat caatcaccat ttccaaggca gcaagaatac gttctttgat tagttctatt
    10981 tcttgtccag accgttcttg gtttgcttcg ctcatcgtac gacactcctt aacacgattt
    11041 ttatttatca tactcttttt ttagctgctt gtaaatgaga atttagaagt aagattctcg
    11101 tttaaattta taaaaatgtt cttattagat gtaatagatt aaatttattc taaaataaac
    11161 ctgattcctc attttttttg tgaacgattt tttatttctg gcaatacgcc agttgttcga
    11221 tacatttttt gttcgttttc atttgcaaac cggccaaata ctgaatgagt tggtaaaata
    11281 ttgataaatg catcgtcgtc gatttcatga acgatctgat caagatcata catttcgtag
    11341 cgggtgatga ccatcatgat catgttccct tgtttttttg aatagccacc gacagacggc
    11401 agtaatgtca ttccacgtac catccgtttt gctagttctt ctgttacctc ttctggcttg
    11461 acggtaacga tcattgctgt tactttttga tgactcgtgt ggatggcatc aattacttga
    11521 gtcattgcat aaatggaaat aatcgtatac aaggcacttt cccagttaaa gaagaagcct
    11581 gcgatgacta caatgatacc atttagaatc agcatgtagt ttccgactgt ttttccagtt
    11641 gtttgtgaaa gtacaagaga gatgatatcc atcccaccag ttgtaaagcc catttttaaa
    11701 gataatccta cgccaagacc taataaaaca ccgcctacta gggcattcat caaaatatta
    11761 tcagtgatct gtccgactgg aagcagcatc gtcatcaccg atacacatac aacattcaca
    11821 aaactaagga tcgttgctcg ttttcctact ttgagaaacc ctaatacaaa cacagggata
    11881 ttcaaaagaa aaataaatgt tcctgtgtct atcttgatat gaaaccattc atataaaagc
    11941 gaggaaatga tttgagcgat cccattcatc cctgcagaaa agacattggc tggaatcaaa
    12001 aaataattta aagcgaccgc agcggtcaat ccggttatca aaataaccac tattttcttt
    12061 aatctttcat ttttatgata cgcagtcata attttactca caagcacctc tcccctactt
    12121 tggtattcca aatgtaagaa taacagttta tctattaaaa taaaatactt cgaactcaaa
    12181 tgagaagaat tttagcaaac aggtaagaat ctgccctaat cctatgataa gatagatgta
    12241 tggtaaatat ttatatgtat atcaggaggg ttcctatggc aaaagatgct agcttcgaca
    12301 ttgtatcaga agtcaataca gaagaagtaa aaaatgcgat tcagcaaaca acaaaagaat
    12361 tgtccaatcg ctttgacttc aaggattcta cagtagaaat caaattagag aacagcaatc
    12421 tagttgtatt gagcgatgat gatttcaaaa tcgaacagat caaagatgta ttatttggta
    12481 agctgatcaa aagaaatgtg ccaatcaaaa atatccattt ttccgaatca gaacacgctc
    12541 taggtggcaa agctcgacaa actgccgatt tggtcaacgg aatcgatcgt gaaaacgcga
    12601 agaaaatcac tacagctatc aaaaatgaaa aactgaaagt aaaagcacaa atccaagaag
    12661 atcagattag agtgacaggt aagaaccgtg atgatctaca agctgtgatt gctttattac
    12721 gtaaacttga cttaccaatc gagttgcaat tcactaacta tcgttaatca aaaaacgagg
    12781 ctgacgtctg tcattcctcg tttttttgat ttactgcttc ttttgataac caaaaacgct
    12841 gtattccttc ttccaattcc ccaacacttc gtacttctct tgctgtttgc caatgttgtg
    12901 ggaataaacg tcgataagaa aaactagaaa atcgttgatc tgccaataca acgattcctt
    12961 gatccgccat gccacgaatc actcgaccac cagcttgcaa gactttgttc attccaggaa
    13021 gctgataagc gtaagcaaat ccaaggtgtt ccttctcatc ataatatgat ttgatcagtt
    13081 cctgctcatg attcatctgc ggcaaaccta cacctacgat catactgccg atcaatcggt
    13141 cccctctcaa atcgattcct tctgaaaaaa tcccgccaag tacgcaaaat ccaatcaatg
    13201 tttcagcggg atttgtttga aattttgtta aaaatcgttc gcgttcttcc tcattcatgt
    13261 ccgtttcttg gattagcaca ttgctgtcgg gatattgctg tctgaatgct tccaccgcaa
    13321 gatctaaata ttgataagaa ggaaagaaaa cgaaataatt cccgaccttt ccatcagcaa
    13381 aagcatgaat cgtctctact aattttgcca gactgtgttc cctttttcga taagtcgtct
    13441 ctaggtaatt tgcgatgaaa acttgctggt ttccttctgg gaaaggactc ggcagtttgt
    13501 aagccagact tttttgtccc cccagtgttt cctgataata agagagtggt gaaaaactag
    13561 cagaaaaaag aatactgctt tttcctttat ccaacgactg ctcaagaaac aagcttggat
    13621 caatacaaaa ctcttttact atcagatcgc gatacgtttt ttcgactgtt gtttcgtagt
    13681 gatcatcata aaattcactg accttcaaaa aatgaagcaa atcaaaataa taaggaagca
    13741 attgttcttg tgatgggtgc tcaggaaatt ctgccagcca ttctttaatt ttttcgctta
    13801 attgataacc tgcctttact aaagattcag ccggtgcttt ttgatgatga taatcccaat
    13861 gatcttcttt tgctatttct cggatcgaat caaattcttt taatagtttg ttgaaacggc
    13921 gatgaagttt tttgaattcc tttggtatag cttccttact tcgtttcgtt ttttcataag
    13981 atagttctgc cgaatacatc tctcgggaac gattcaccaa attatgtgct tcgtcaatca
    14041 aaaacagata gtcctcgttt ttttcatctt caaaaaaacg tcttaaataa acagttggat
    14101 caaataaata attgtaatcc ccaatgatta cgtcacacca gatactgaca tctaatgata
    14161 gttcgaaagg acaaagtgta tgctttcttg cataagtttc gatgacttca cgagtgatct
    14221 gattctcatg attcaaaaga tcccataaac cttcattaat ccgattgtaa taaccattgg
    14281 catatgggca ctgatctgga ttacaggtcg tctcatctaa aaagcagatc ttgtctttcg
    14341 ccgtcaaagt gacacttttt acttcagctc ctgtatcttt taatgctgtc atagcatctt
    14401 cagctacttg tctagttatt gttttagccg ttagataaaa gatcttttct ccctcttctt
    14461 cccctacagc tttcaaagcg gggaataacg tagagattgt ttttcctgtt ccggtaggtg
    14521 cttctacaaa caatttctgt ttcgtacgga tcgttttata agcagctacc gctagttcac
    14581 gctgaccttt cctatactca tcatggggga atttcaaagc catcaaagag gcattgcgta
    14641 cagtgcgcca gttttcttga aaaacaagcc attctttata atcttcagtc agctttttaa
    14701 aaaaagtttc taactgctct cttgtctgat gttcaacctt tctcgtgatc acttcttctg
    14761 ccgtctggaa gtaagtcaat tgcagattta tttcttgtaa atcatgttct tgactataga
    14821 tataggcata cacccttgct tgatgaaaga aaagttccac ttgttctggt tctaaatctt
    14881 caaatcgtgg ttcggaggtt ttgatttcat ctatataata aataccgtct cttttgaaaa
    14941 taccgtctgc acggccttca acgatcaggt aatattcatc taattgaact gttgttttta
    15001 aaaagacttc tttttcatag ttttctcccg catctttttg gagcttgcgg tggattctcg
    15061 ctccttctaa tgcagtatgg ttactttttt tacgattgtc gatacttcca cgtcgcaaaa
    15121 taaattctac taagtgccgc acagatattt ttgactgttt gatcatttcc accacctctt
    15181 tctctctaag attacctgaa aaaacctagt ttagcaaaaa gagctctctt tccaaacata
    15241 aaaaaacacc ctgctgatta ggcagggata taggatatag aatttggatt tttggatgaa
    15301 tctaatgtaa caaggacata ttaaaatgct atgaaaactt actttccttc tctttagaat
    15361 atccaaatac ttttcatata gtctgataat tcagataagt aaagagaaat aagtacctgt
    15421 ttttgtttcc tagcaatgaa tgcgttatac taatggatga aagaagtttt ggctatcaag
    15481 gaggaatgca catgcatatc gaaagattaa aaacaggaat gattgaagaa aactgttatt
    15541 tggtctacaa cgaagaagca cttttgatca ttgaccctgg agaagatgcg gaacgaatca
    15601 aacaagaaat caaaaaaaca aaccaacagc cggtagcgat tttgcttacc catactcact
    15661 acgatcatat cggtgcagtg gaacctttac gcgcgtatta tcaaatccca gtttatgtca
    15721 gcccactaga gcaaaaatgg ctaggtgatc cgattttgaa tctttctgga ctcggtagac
    15781 atgatgatat tgctgatgtt attgtctcgc ctgcagaata tgaatttgaa atgaaaactt
    15841 atactttagg aaacatgtct tttcgcgtcg tccctacccc agggcattct ataggaagcg
    15901 tcagctttat ttttgatgat tttgtcgttt ctggcgacgc cctgtttaga ggaagtatcg
    15961 gtagaaccga tctgtataca gggaatttag aacaattact tcatagtatt cggacccagc
    16021 tgtttgtatt acctgatgaa tttgcagtct atccaggaca tggcgatgca acgacgattg
    16081 aacaggaaaa aaggacaaat ccttttttca atcgttaaaa cgacttagga ggagaataaa
    16141 atgacagaaa ctacacttta tatcgtacga cacggaaaaa cgatgttcaa tacaattgaa
    16201 cgagtacaag gctggtgtga tacacccctc acaaagcaag gacaagaagg cattcattat
    16261 ttaggtcgcg ggttaaaaaa tgtcgacttt tcatttgctt attctagtga cagtggtcgt
    16321 gcaatcgaaa ctgcccgtat tattctagga gaacattcta agggaaaaga aattccttat
    16381 tttattgaaa aaaggattag agaatggtgt ttcgggtctc ttgaaggtgg atatgacatg
    16441 gaaatgtggg gtgtgatccc tcgtgtattg aattttaaaa catatgatga aatgtttacc
    16501 acagaagttt cttttgaaca aattgctaat gctgtataca acttagatac cgctgggtgg
    16561 gcggaaactt atgaaacatt gcgagaccgt gtatggacag gatttgaaga tatcgcccat
    16621 cactgcgaaa aaaatggtgg tggaaatgtt ttagtcgtat ctcatggttt gacgatcgcc
    16681 tttttgctta atctgatcga tcctaaacag cctgttcgtg ctgggctttt aaatggcagc
    16741 gtaacaaagg tcacttatgc aaacggtcga ttttctatcc acggcatcaa tgatacgtct
    16801 tatatcgata acgggaaaac acatagtcgc gactcgtttc tataatgggg atattttaat
    16861 ttttaccaga gaatcataaa aggcaaagtc tgaacgaggg ctttgtcttc ttttttctct
    16921 tcgtgtactt atctgcttta tttctcaagt ttttcttaat attaaaaaac agattgggtt
    16981 tctcaaaaaa taacggtata ctataagatg tatcttaatt gggaaggaag gaatttatgt
    17041 tttttgcaaa tattatcaaa gcagtgattc tcggtattat tgaaggaata acggaatggc
    17101 tgcccatcag tagtactggt catttgatcc tagcagatga attcatcaaa ctggatgcca
    17161 gttcacaatt tatgtcgatg ttcaatgtcg ttatccaatt aggggcgatc ttggctgtcg
    17221 tcgttcttta ttttcacaag ttaaacccat tttctccaag taaatcaacg gtagaaaaaa
    17281 aagatacgtg gacactttgg tttaaagtga tcgttgcttg cataccagcc atcatcattg
    17341 gtttgccttt ggatgattgg ttagaagcaa atttccacaa gttcttacca gttgccattg
    17401 ttttgatcgt ttacggtatt gcctttatta ttgtcgaaaa acgcaacaaa aacaaaacac
    17461 ctcgttggac ttctctgaat gactttacgt accaagcagc tcttcttgtt gggttattcc
    17521 aagtcttggc acttgttccc ggaacatccc gttctggcgc aacgatccta ggatctatct
    17581 tgattggtgc ttcgcgtttt gtcgctacag agttttcatt tttcttaggg attcctgtca
    17641 tgtttggcgc aagcggcttg aagatcgtca aatatttagc tgaagggaat tcattcaaaa
    17701 tggaagaaac tgttgttctt cttgtaggaa cattcgtatc ttttgtggtt tccgtcttag
    17761 ctatcaaatt cttactaagc tatttgaaga aaaacgactt taccgctttt ggctggtatc
    17821 gtattatcct tggtatcatt ttgattggtt attggtttat tgcaatgtag aaaaaaccag
    17881 aacacttctc t
//
LOCUS       DACDRK010000050        17588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDRK010000050 DACDRK010000000
VERSION     DACDRK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.050"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            31..996
                     /locus_tag="IWJ77_001847"
     CDS             31..996
                     /locus_tag="IWJ77_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9453446.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(1095..2267)
                     /locus_tag="IWJ77_001848"
     CDS             complement(1095..2267)
                     /locus_tag="IWJ77_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9453447.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(2254..3399)
                     /locus_tag="IWJ77_001849"
     CDS             complement(2254..3399)
                     /locus_tag="IWJ77_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9453448.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(3413..4345)
                     /locus_tag="IWJ77_001850"
     CDS             complement(3413..4345)
                     /locus_tag="IWJ77_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453449.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            4462..5541
                     /locus_tag="IWJ77_001851"
     CDS             4462..5541
                     /locus_tag="IWJ77_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9453450.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            5538..6167
                     /locus_tag="IWJ77_001852"
     CDS             5538..6167
                     /locus_tag="IWJ77_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9453451.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(6277..6504)
                     /locus_tag="IWJ77_001853"
     CDS             complement(6277..6504)
                     /locus_tag="IWJ77_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9453452.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(6607..9195)
                     /locus_tag="IWJ77_001854"
     CDS             complement(6607..9195)
                     /locus_tag="IWJ77_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9453453.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(9215..9874)
                     /locus_tag="IWJ77_001855"
     CDS             complement(9215..9874)
                     /locus_tag="IWJ77_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9453454.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(9923..10426)
                     /gene="trmL"
                     /locus_tag="IWJ77_001856"
     CDS             complement(9923..10426)
                     /gene="trmL"
                     /locus_tag="IWJ77_001856"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9453455.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(10647..11513)
                     /locus_tag="IWJ77_001857"
     CDS             complement(10647..11513)
                     /locus_tag="IWJ77_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9453456.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            11622..12263
                     /locus_tag="IWJ77_001858"
     CDS             11622..12263
                     /locus_tag="IWJ77_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9453457.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(12325..13686)
                     /gene="mgtE"
                     /locus_tag="IWJ77_001859"
     CDS             complement(12325..13686)
                     /gene="mgtE"
                     /locus_tag="IWJ77_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9453458.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPLKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(13704..14603)
                     /locus_tag="IWJ77_001860"
     CDS             complement(13704..14603)
                     /locus_tag="IWJ77_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9453459.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(14605..15402)
                     /locus_tag="IWJ77_001861"
     CDS             complement(14605..15402)
                     /locus_tag="IWJ77_001861"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9453460.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(15380..16084)
                     /locus_tag="IWJ77_001862"
     CDS             complement(15380..16084)
                     /locus_tag="IWJ77_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9453461.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            16215..16790
                     /locus_tag="IWJ77_001863"
     CDS             16215..16790
                     /locus_tag="IWJ77_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9453462.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            16908..17585
                     /locus_tag="IWJ77_001864"
     CDS             16908..17585
                     /locus_tag="IWJ77_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9453463.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         5197 a         3783 c         3133 g         5475 t
ORIGIN      
        1 tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat
       61 ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa
      121 aataagcata ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt
      181 gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc
      241 gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat
      301 actttctatc agatggaagt agaattccca gttgcttata ttccagcagg ttctggaaat
      361 gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta
      421 gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa
      481 aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca
      541 aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta
      601 ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga
      661 ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt
      721 ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc
      781 gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa
      841 aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca
      901 acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc
      961 acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta
     1021 catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt
     1081 tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta
     1141 cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg
     1201 aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta
     1261 ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag
     1321 tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg
     1381 atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg
     1441 atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag
     1501 gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa
     1561 ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg
     1621 tttcgtttac tgatctcttt gcgattaaac gctagattga tgacgctgaa accgctaaaa
     1681 atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc
     1741 atccgctgac tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt
     1801 gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc
     1861 aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt
     1921 atcttgatcg tcgccatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt
     1981 aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca
     2041 tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc
     2101 gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca
     2161 atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc
     2221 gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct
     2281 ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt
     2341 ggtataaaaa ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa
     2401 gttgacgaga tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg
     2461 ttccgacccc atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct
     2521 atccttttgc accagattca tttttggtat caaattacca accaatgtac ctaacaaaat
     2581 cgcacacaac gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc
     2641 tacttgatag ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt
     2701 aaagcttgct gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa
     2761 gcgaatacca tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat
     2821 cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat
     2881 attacgacct gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg
     2941 cgcgagatca acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa
     3001 aaattgatcc atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag
     3061 cgaaacagat gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc
     3121 attcgcttct acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac
     3181 ttgttctaag actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat
     3241 tccagtatta atcgtttcaa gattgccacc ttgatcaatc tcgccaaagg ccgccataaa
     3301 catcaatcct aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag
     3361 cagtattttt gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc
     3421 tcaaatcctt gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta
     3481 tttttccgta actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat
     3541 ttagttcagt agttgttact tggagctgac cttttttata agcgattcca tcagaagcta
     3601 ttgttcgctg aatcgctgac aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac
     3661 gatccgccgt ccgtaccata tcttttagag attctttggt tcctttagca atcacctgtc
     3721 cttgatccat gatcacgatc tggtcgcaaa gaatttctac ttcttccatg taatgtgttg
     3781 tatagacaat ggttgctcct tgtcgattca gctccttgat actttctaaa attttattcc
     3841 gactttgggg gtctacagct actgtcggct catctaaaaa aattaaccgc ggcttgtggg
     3901 caatcccaca tgcgatgttc aaccgacgtt tcaagccacc actaagttct cctggacgaa
     3961 acgaaacata tttttctaat cccactaatt gaatcacctc ttcaatcaag gctttccttt
     4021 tctctttttc ggaaacatat aaaccgcaaa aataatcgat attttcttgc acacttagtt
     4081 cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat tgccttgaca gcataatttt
     4141 caggagtcat tgttgtcccg aacaatttaa tggttccttt gtcataattt aacaaagaaa
     4201 gcatacagtt gatggcagtc gatttcccac tgccgtttgg acctaacaga cctaagactt
     4261 ctccttcttg tacttgcaga tcaaaatgat tcaatgctac aaaatctcca tagcgtttga
     4321 ctaatttatc aatttcgata atcatcatga aaaactcctt ccaaatttct tgttgatctg
     4381 actataataa agtgcaagaa aagaaactag tgacgattgg ctaaaccaca catgacattt
     4441 gtcacaatga aggagtctgc tatgcataaa tctcggctac tcaacgaaac tagctttcta
     4501 ctggttatct cattgctttt gctggtaagt caaggattcg ttgctatgca agttatctat
     4561 ttattgatcg ccgttttatt cactggtgtc agttatttac tgcctacaaa gccaaattgg
     4621 attttccaag ttattctatt tcttttatgt gcctttttac ctgatcttgt cctgtttctg
     4681 ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc ttgattactt gtttttgtta
     4741 ggttttttgt tgatttcgac ggttggtaat catcccccca tcaaggtcgc ctttgtcgta
     4801 attttgctgt ttttttcctt ttacttacga aaaaaagatc aacgaacgac cgatcttgag
     4861 aaaaagtaca ttgtctccaa agatactcat tgggaaaaac aacaagagtt atcggaacaa
     4921 aataaagcat tggccctgtc acaagaaaca caattagcct tgcaaattgt ggaagaacgg
     4981 aaccgaattg cccgagacat ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta
     5041 caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc attagaaaat
     5101 ttaagtgcca ccgttcacac tgggatggat cggattcgtc aaagtgtcca tgacctccat
     5161 gagacatcga tctcttttca acactcctta gagtttctga tcgctgattt tccttcttct
     5221 gttacgattg aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta
     5281 atggtcatca aagaagcctt aaccaacagt atcaaacata gtcatgccaa aaccgttgtt
     5341 ttacattttc gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga tggcaccagc
     5401 ccacaaaaaa attctgctcc cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa
     5461 caaaagggac aagtccatat tttccaagaa aaccagcaat ttcagttaac gatcatttta
     5521 ccaagggagg gacaccaatg ataacagtag tcattattga tgatgatcca tttgtgacga
     5581 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
     5641 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
     5701 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
     5761 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
     5821 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
     5881 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
     5941 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
     6001 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
     6061 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
     6121 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
     6181 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
     6241 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
     6301 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
     6361 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
     6421 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
     6481 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
     6541 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
     6601 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
     6661 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
     6721 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
     6781 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
     6841 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
     6901 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
     6961 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
     7021 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
     7081 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
     7141 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
     7201 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
     7261 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
     7321 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
     7381 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
     7441 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
     7501 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
     7561 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
     7621 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
     7681 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
     7741 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
     7801 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
     7861 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
     7921 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
     7981 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
     8041 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
     8101 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
     8161 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
     8221 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
     8281 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
     8341 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
     8401 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
     8461 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
     8521 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
     8581 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
     8641 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
     8701 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
     8761 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
     8821 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
     8881 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
     8941 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
     9001 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
     9061 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
     9121 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
     9181 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
     9241 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
     9301 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
     9361 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
     9421 cgaataaaag tgggccatct ttatgtcggg caaccgcatc tgctacaaca gtttcgatac
     9481 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
     9541 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
     9601 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
     9661 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
     9721 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
     9781 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
     9841 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
     9901 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
     9961 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    10021 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    10081 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    10141 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    10201 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    10261 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    10321 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    10381 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    10441 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    10501 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    10561 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    10621 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    10681 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    10741 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    10801 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    10861 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    10921 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    10981 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    11041 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    11101 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    11161 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    11221 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    11281 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    11341 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    11401 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    11461 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    11521 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    11581 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    11641 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    11701 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    11761 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    11821 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    11881 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    11941 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    12001 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    12061 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    12121 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    12181 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    12241 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    12301 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    12361 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    12421 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    12481 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    12541 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    12601 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    12661 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    12721 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    12781 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    12841 tgcctttagt ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    12901 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    12961 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    13021 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    13081 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    13141 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    13201 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    13261 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    13321 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    13381 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    13441 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    13501 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    13561 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    13621 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    13681 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    13741 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    13801 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    13861 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    13921 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    13981 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    14041 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    14101 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    14161 aatccatgct ttgcaaaaag catcaagcca gatgtatccc gatctagtcg cgtcaccaca
    14221 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    14281 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    14341 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    14401 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    14461 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    14521 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    14581 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    14641 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    14701 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    14761 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    14821 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    14881 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    14941 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    15001 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    15061 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    15121 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    15181 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    15241 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    15301 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    15361 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    15421 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    15481 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    15541 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    15601 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    15661 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    15721 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    15781 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    15841 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    15901 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    15961 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    16021 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    16081 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    16141 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    16201 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    16261 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    16321 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    16381 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    16441 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    16501 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    16561 taatccaaac cggaaagctt acaacaaaac gagctgaatt ccctattgaa gaaggacttt
    16621 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
    16681 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
    16741 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
    16801 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
    16861 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
    16921 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
    16981 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
    17041 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
    17101 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
    17161 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
    17221 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
    17281 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
    17341 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
    17401 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
    17461 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
    17521 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
    17581 attaaaaa
//
LOCUS       DACDRK010000051        17344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDRK010000051 DACDRK010000000
VERSION     DACDRK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.051"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..368
                     /locus_tag="IWJ77_001865"
                     /pseudo
     CDS             1..368
                     /locus_tag="IWJ77_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            548..1489
                     /locus_tag="IWJ77_001866"
     CDS             548..1489
                     /locus_tag="IWJ77_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9453464.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="IWJ77_001867"
     CDS             1558..2289
                     /locus_tag="IWJ77_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9453465.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="IWJ77_001868"
     CDS             2433..4613
                     /locus_tag="IWJ77_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9453466.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="IWJ77_001869"
     CDS             4633..5115
                     /locus_tag="IWJ77_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9453467.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="IWJ77_001870"
     CDS             5131..5892
                     /locus_tag="IWJ77_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9453468.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="IWJ77_001871"
     CDS             6023..7357
                     /locus_tag="IWJ77_001871"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9453469.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7785..8270
                     /locus_tag="IWJ77_001872"
     CDS             7785..8270
                     /locus_tag="IWJ77_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9453470.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8263..8946
                     /locus_tag="IWJ77_001873"
     CDS             8263..8946
                     /locus_tag="IWJ77_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9453471.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9072..11717
                     /gene="polA"
                     /locus_tag="IWJ77_001874"
     CDS             9072..11717
                     /gene="polA"
                     /locus_tag="IWJ77_001874"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9453472.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11762..12598
                     /gene="mutM"
                     /locus_tag="IWJ77_001875"
     CDS             11762..12598
                     /gene="mutM"
                     /locus_tag="IWJ77_001875"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9453473.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12562..13191
                     /locus_tag="IWJ77_001876"
     CDS             12562..13191
                     /locus_tag="IWJ77_001876"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9453474.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13243..13575
                     /locus_tag="IWJ77_001877"
     CDS             13243..13575
                     /locus_tag="IWJ77_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9453475.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13887..14378
                     /gene="nrdR"
                     /locus_tag="IWJ77_001878"
     CDS             13887..14378
                     /gene="nrdR"
                     /locus_tag="IWJ77_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9453476.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14401..15774
                     /locus_tag="IWJ77_001879"
     CDS             14401..15774
                     /locus_tag="IWJ77_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9453477.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15774..16700
                     /gene="dnaI"
                     /locus_tag="IWJ77_001880"
     CDS             15774..16700
                     /gene="dnaI"
                     /locus_tag="IWJ77_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9453478.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16829..17269
                     /locus_tag="IWJ77_001881"
     CDS             16829..17269
                     /locus_tag="IWJ77_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9453479.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5908 a         3032 c         3820 g         4584 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tcccgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7441 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7501 gaagctgttt ttcttggctg ttcttattac ttgatgcagg gcagcttttt cacgacctca
     7561 gataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaaat gaaaaatatg
     7621 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7681 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7741 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7801 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7861 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7921 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7981 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     8041 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     8101 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8161 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8221 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8281 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8341 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8401 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8461 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8521 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8581 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8641 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8701 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8761 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8821 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8881 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8941 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     9001 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     9061 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9121 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9181 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9241 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9301 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9361 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9421 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9481 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9541 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9601 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9661 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9721 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9781 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9841 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9901 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9961 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
    10021 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
    10081 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10141 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10201 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10261 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10321 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10381 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10441 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa aaccaagaag
    10501 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10561 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10621 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10681 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10741 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10801 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10861 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10921 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10981 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    11041 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    11101 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11161 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11221 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11281 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11341 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11401 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11461 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11521 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11581 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11641 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11701 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11761 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11821 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11881 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11941 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    12001 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    12061 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12121 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12181 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12241 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12301 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12361 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12421 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12481 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12541 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12601 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12661 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12721 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12781 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12841 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12901 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12961 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    13021 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    13081 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13141 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13201 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13261 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13321 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13381 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13441 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13501 gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13561 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13621 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13681 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13741 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13801 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13861 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13921 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13981 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    14041 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    14101 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14161 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14221 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14281 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14341 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14401 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14461 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14521 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14581 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14641 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14701 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14761 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14821 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14881 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14941 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    15001 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    15061 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15121 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15181 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15241 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15301 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15361 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15421 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15481 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15541 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15601 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15661 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15721 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15781 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15841 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15901 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15961 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    16021 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    16081 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16141 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16201 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16261 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16321 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16381 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16441 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16501 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16561 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16621 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16681 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16741 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16801 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16861 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16921 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16981 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    17041 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    17101 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17161 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17221 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17281 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17341 tggg
//
LOCUS       DACDRK010000052        17251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDRK010000052 DACDRK010000000
VERSION     DACDRK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.052"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            88..327
                     /locus_tag="IWJ77_001882"
     CDS             88..327
                     /locus_tag="IWJ77_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453480.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(689..949)
                     /locus_tag="IWJ77_001883"
     CDS             complement(689..949)
                     /locus_tag="IWJ77_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453481.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1134..1631)
                     /locus_tag="IWJ77_001884"
     CDS             complement(1134..1631)
                     /locus_tag="IWJ77_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9453482.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1761..2471)
                     /gene="budA"
                     /locus_tag="IWJ77_001885"
     CDS             complement(1761..2471)
                     /gene="budA"
                     /locus_tag="IWJ77_001885"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9453483.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2484..4148)
                     /gene="alsS"
                     /locus_tag="IWJ77_001886"
     CDS             complement(2484..4148)
                     /gene="alsS"
                     /locus_tag="IWJ77_001886"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9453484.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4356..5018)
                     /locus_tag="IWJ77_001887"
     CDS             complement(4356..5018)
                     /locus_tag="IWJ77_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9453485.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5028..5843)
                     /locus_tag="IWJ77_001888"
     CDS             complement(5028..5843)
                     /locus_tag="IWJ77_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9453486.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6104..6547)
                     /locus_tag="IWJ77_001889"
     CDS             complement(6104..6547)
                     /locus_tag="IWJ77_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9453487.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6681..7019)
                     /locus_tag="IWJ77_001890"
                     /pseudo
     CDS             complement(6681..7019)
                     /locus_tag="IWJ77_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(7007..7384)
                     /locus_tag="IWJ77_001891"
     CDS             complement(7007..7384)
                     /locus_tag="IWJ77_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9453488.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7608..8801
                     /locus_tag="IWJ77_001892"
     CDS             7608..8801
                     /locus_tag="IWJ77_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9453489.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(8967..9389)
                     /locus_tag="IWJ77_001893"
     CDS             complement(8967..9389)
                     /locus_tag="IWJ77_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9453490.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9972..10466
                     /locus_tag="IWJ77_001894"
     CDS             9972..10466
                     /locus_tag="IWJ77_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453491.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10628..11800)
                     /locus_tag="IWJ77_001895"
     CDS             complement(10628..11800)
                     /locus_tag="IWJ77_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9453492.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12006..12821)
                     /locus_tag="IWJ77_001896"
     CDS             complement(12006..12821)
                     /locus_tag="IWJ77_001896"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9453493.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(12971..13417)
                     /locus_tag="IWJ77_001897"
     CDS             complement(12971..13417)
                     /locus_tag="IWJ77_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9453494.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13414..14418)
                     /locus_tag="IWJ77_001898"
     CDS             complement(13414..14418)
                     /locus_tag="IWJ77_001898"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9453495.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14458..14787)
                     /locus_tag="IWJ77_001899"
     CDS             complement(14458..14787)
                     /locus_tag="IWJ77_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9453496.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(14913..17240)
                     /locus_tag="IWJ77_001900"
     CDS             complement(14913..17240)
                     /locus_tag="IWJ77_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9453497.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4962 a         3427 c         3030 g         5832 t
ORIGIN      
        1 tataaaagac ttgatagttt tgcattgatt ttgcctaata ctgagcatag gtgtatgcta
       61 aacgatacta taaggagtgt gtacataatg aaaagaattc tgtttatcag tttatcaaat
      121 ttattgttac ttactgcttg tgggacttct gcatcaaaga acataccttc agatacgcaa
      181 atttcgaata gtatcgattc aacaatttct acaaatgaga aaaattctcc ggtagaaact
      241 tccgatataa aagtttctac ctctgcaaaa aacagggtaa agaaactgtc agcacgtctc
      301 ctttatttca aacaaactct gaattagctt cttcatcttc ttcagcaaat aatgatgata
      361 gtttcgcgtt tactcctaac taaatgcaac ctttacaaat gctttacgat aacaagcctg
      421 agctaaatga tcctgatatc agttttgcat tttttgaaat gattggatcg gatgatcttt
      481 ttaaaactac tagtttgtct atccgaaaat aggacagttc tggcagtata ggtttttatc
      541 gagtttctcc accaggcctc gttacagaga cagattctct aggcgaacct tttgaataaa
      601 tcaaaaatca tgaacaatca ctgccaacta aataaagtgc cttcaaaagc ttggtcttct
      661 cgcttttgaa ggcactttat ttaaatcttt atgaaactaa agtatgttca tatttgttgc
      721 tatagttata tagatattct gttgtttcca actcataaac gtgagtatat tttgccacta
      781 cttttccgca tttttctaat atttcattgc gatcgacgtc atgatattcg tctacgcaga
      841 tcacaacaca attttctaat ttactgatta cttctccaac cactttttgt ttcaagccaa
      901 taggttggca aatatatttt tttcctaaaa tgatagtttc gttattcata tttcatccat
      961 ccttaatgtt ttttcattat tcaaaatgat aataccatta aaatatgatt gtgaaaatca
     1021 ataacacttg tgattttata aaaaaataaa aaaaccgaaa aacagaaatg accactgtct
     1081 ttccggctct ttttattctt gatttctctt cttttttgtt tcaatagacg attttagtta
     1141 ttaataaatg aaaaagcatt tatattaaaa tttgctaata aaagatctac atatttttct
     1201 agaaatgctt gatcgatttc atcataccct ttcgttactc cgcaatcgat atctaaaaca
     1261 ccgattaaat taccttcttt taccattggc actacgattt ctgattgtgc ggctgaatcg
     1321 caggaaatgt aatttgcatg ggttttgaca tcatcaacga tcaatgtttc ttgtttttca
     1381 gctgattctc cacaaactcc ttttcccatc ttgatacgtg tgcaagaaac tcgcccttgg
     1441 aacggtccta aaatcaattc attaccatcg aataaataat atcctgcaaa gacagtctca
     1501 ggcaaagtct cattcaacaa tgcagatgaa tttgctaaat ttgctaacca gttatcttct
     1561 gcttctaata atccttgttg ctgtaataat aaaatttcgt atgcatcttt tttctgtttt
     1621 tctgtccaca tgataaaaac cgctcctttt tgatctatgg agaagattat agctcagttt
     1681 ttttgtaaac gctaggtttc ctcctcaaaa aaacgagata cgtcaggtta atcaccttga
     1741 acgcatctcg ctgtcttttt ctattctgct tgttggattt ctgctgctaa atcagcataa
     1801 tcaatcgttt cgtgaacaaa agtcggatca tcaactggaa aatgctgtac aagggacgag
     1861 atgcggctaa tctcaacttt tccttcagcc acttcaaaat ccattacatg tccgccaaat
     1921 gtatgtgtat catcgataaa atgcaggtga aatcccgctg cagcaactcc ttggaataat
     1981 tcaggcgtaa agaacccgac taatgttcct tggatattgc tttgttggaa ttctggttga
     2041 gactctgaaa tttttgctag tcttctgtaa ggtttttctt gcttcggcat gatacggatg
     2101 tgcatatttt tgaataatcc agttatcttc actgccaaaa ataggttttc cccggcttct
     2161 cttactaata tgtctttttt caattgttct ccatctgcag tttttgagac agaaaaacta
     2221 gtatctggcg taaaatctgt aattgctgca tagggggtaa gttcacttcc atctaattgg
     2281 gcaactgagc catctgatct tccttgataa gctacgccat ctaaaaagat gacttcacca
     2341 tcaagcccat gcaatgttcc caatcccaga gttccttttt ctaatatatg agcaatcgtt
     2401 tctgttccat ccatcaagcc ggccatcaaa gctcctaatg tcccgtgctg gtataagatt
     2461 ttttcagtca tgtcgacact tccttaatag aattgatctg gtaagattgt ttctcccaat
     2521 ttctcattat cacggtaatc aatcggaata tcaataatta cgggtccttc tgtcgcaaat
     2581 ccttcttcta aagctttttc taattcctct acacttgtag ctcggattcc tttagcgcca
     2641 aaagcttctg catatttcac aaaatcaact ggaccaaagt ctacacctga cgcccgctga
     2701 tatttcattt tttcttggaa ctctaccata ttgtagtgtc catcattcca aatcaaatga
     2761 acgatattca attttttacg taccgccgtt tctaaatcct gggcagagaa taaaaatccc
     2821 ccatcgccgg aaactgatac gatttgtgta tttggacgca ccaatgcagc agaaatcgcc
     2881 catggcaatg caacaccaag cgtttgcata ccattactga ataacaaatg acgaggttca
     2941 taacttctaa agtggcgtgc catccaaata taatgactac ctacatctac ggtaacagtc
     3001 atatcatctg ttacttttga ctgaagcgtg ttaataactt ctaaaggatg caagatacct
     3061 gcttctcctt ttgattgaac aatgtcccgc tccgttaatt ttgcttgaag agatgctaaa
     3121 tattcttttg catcttctga cacttgttga ggctctaatg aacctgtcaa taaatcaaga
     3181 gtggctgaga tatcaccgat caattctctt tcaggctgca taaaaggatc gatttcagct
     3241 ggcgcttcat cgatcacaat gatccgcgca tctttttccg cattccagtt tcttgcttca
     3301 tattcgattg gatcatagcc aattgcaata actagatcac ttcgtttcaa aagcatatct
     3361 cctggttggt ttctaaataa acccacacga ccaaagaaat gcgcttctaa ttcacgtgag
     3421 atcactccag ctgcttggaa tgtctctaca acaggcaatg ctgtcttttc aactaattgc
     3481 ctgatggcta acgtttcttt ttcacttgat cctcgcatac ctactaatag taccggtagt
     3541 tttgcttctc taatgcgttc agctaggtaa cggatatcat ctgcagaagc agaacctaat
     3601 ttaggatcgc tcaatggctt gatgacatta ccttggacag gtgcatccac tacatcttgc
     3661 gggatactga taaagctcgc gccttttttt gaactcttag ccatacgata agcattggca
     3721 atcacttctg aaattgtttc tggatcttgg atttctgcac tgtattttgt gattggctgg
     3781 aacagtgctg cattatccat gctttgatgg gtcaacttca atagatcgct tcgttttact
     3841 tgtccggcaa ttgctaaaac tgggtcacct tctgctgtcg ctgttaccaa acctgtagct
     3901 aagttactag caccaggacc acttgttgcg ataactactc ctggctctcc tgtaatacgt
     3961 ccgattgctt gtgccataaa tgcagcattt tgttcgtgac gagtcacgat taattcagga
     4021 ccttgatcct ctaattcatt aaatacacca tcgatttttg cacctggtat accgaataca
     4081 taatcaacat gatgattaat caaactttct actacagatt tcgaaccttg tttcattttt
     4141 tcagccattg aacatcgtcc tttttatgct ttatttttaa ttattagtta gaaataatca
     4201 caaataagtg atacagtttc taaacctgtt tcatacaaaa gtatagcacg ttttatttca
     4261 tttgtttagc catatgtttc aaaaaatgaa aaagaatgac agtttttaca taaaaaaaac
     4321 tgtcattctt aattgaaagt ttcaatttat aaagtctaca gatccatagt tctcatcaat
     4381 ttacgattgt tgaagtaaat caaaccaaat acgatagaag gacctagtag aatacaccat
     4441 tggggcttta cgaaaaagaa gagaaacaaa cttgtcaata gtaaaaaatc tagtaaactc
     4501 acataccatt ttttcaacgt atagtagaac gctatccgta gaacttgatt tggtttcgac
     4561 gctggatttc tcacctgaaa ataccagcag ttggtgctaa aggaaactga taaacaagca
     4621 agtaaaacaa acaacggaat caaccatttt ccataagaaa aacgtataaa aaagaaacaa
     4681 tcaaaaatca ttatcacact aactatccaa gcaaatagcc aataaaaaaa tccctttagc
     4741 caaaattggc gaaacatctt tatgaacaaa cgtactggtg aaaaactatt ttcttttgct
     4801 aattcttctg tcatacctat catcacgatg caacctggac caataaaaag taaagaaaaa
     4861 ataaaggaaa agacattcct tccatctatc gacaaaagga acgcagctgc tatatacaag
     4921 atattgacac accaaaaaca aaggctactt gccaatgatt gatacaacca ataaaagaat
     4981 tccatataaa catttttgtc aaaacgctgc tctttcataa tcacctccta ttccttcatt
     5041 cctgatgaag cgatcccttc tacaaaatat ttttgcatcg ctaaaaatat aaaagcaaca
     5101 ggaagaacag aaacaactga ggcagccatg attaatgcgt actctgcatc aaatagccca
     5161 ataaacattt tcaaccctaa ctggattgtt ttattttccg tagaagacaa ataaatgaat
     5221 ggccccatat aatcgttcca tgtattaacg aatgtgatga tcgtcaaact ggcaatcgcc
     5281 ggttttgtaa gaggtaaaat gatcttgcga tagattcccc actctgataa gccatcgatc
     5341 cgcgcactct cacataaaga atctgggata ctactgtaat attgcttcaa caagaacaca
     5401 ccaaaagcat tgaacgcttg taatagcacc aacgaccaca acgtatctgt taaacccact
     5461 tttctcatca taatgaactg cgggatcata taagactgcc aagggacagc aatcgtgccg
     5521 atatatgcta aaaataaaat atcgcgccct ttaaaattta gtttagaaaa cccgtaagca
     5581 gcaaaactag aagtaaacaa ctgaatcaac gtgatcacaa cgcttaaaaa agctgtattc
     5641 ttaaaaaatg tcactaaagg aatacgagac caaataacca gataattctc taaatgccaa
     5701 gttttaggga tccattgaat cggaatcgtg aacacttcat tatttgcttt gaatgaagca
     5761 gaaaccatcc ataaaaaagg aatgatcgtc aataaagcta atacaatcag cagaatcata
     5821 cccaaccatt gaataaaggg catgtttttt gaacgaattt ttgctttttt tctttcattt
     5881 cgaacacctc caaagttata atcttcattt ctttgtaatg attacaatcc agaacaacgg
     5941 ttattatagc tgattcattt ccgttcttct tatttttgta agcgcttatt taacaagtta
     6001 tgtaaatctc tttatttttt tgatttaaaa atgaaaaaat acaaaagcaa gactgtcata
     6061 cgtctcgctt ttgtattgaa atttcttcga ttagagatac ttattactgt tcaaaagctt
     6121 ctttgaaacg gtgaatcaat tcattgacta caggtgataa aatccgattc tttttccaga
     6181 ctagcatgca gttcgtttca atcattgggt caagcggaac gaatgtcact cgctttgaat
     6241 gatgacttac agcaccttct atgactaaag cagcacctac ctgattctca attagcgcaa
     6301 atacattgaa aatcaaattg tacgttcctg tgatcagcaa ctgctccttt tttatgccta
     6361 accagtcagc aaacattcgc tgtatttctg gacgctttga gatcaaaaga ggtacattcg
     6421 caaagtcttc ttttgttatc acgtcttttt ttgctaaaac agaattttct aaaatgagca
     6481 atccccattt ttcctttctt ggtaacaaaa tcgtttcgta tttctctgta tctactggat
     6541 ctagcaaaat cgctaaatct aacaggcttt tttctagtca ctccgtgata tcatcacttg
     6601 ttccgctgtg gatatgaaat tgaacgctgg gataatcacg gatcaacttt tctagtaata
     6661 gtgccaatgt attcgaacta tcagcttcca cacagccgat cgtaaatcgg ccgctgaata
     6721 attgtttttt tctgttctcg aattcttgag tggtttgatt cgttaaatct aaaatttctt
     6781 ctgctcggct tttttagaaa ataacctgct tctgtcaaga tcattttgcg gttttcgcgt
     6841 acaaacagtt ctgttcctag ttactcttct aactccttca gttgtcggct taaggttggt
     6901 tgtgtaagat gtagaacttc tgcggcttga gagattgttc cttcttctgc aattgtccaa
     6961 aaatatttta gtaagcgtaa ctccatagcg atgctccttg atttttttac ttgatacatt
     7021 ccattttatt tctagataac acacgatcat atttcttgat ttttccttgt aaacgttcca
     7081 aacattcttg catttcagca atttttttcg tcaaaagttc actttcttca atcaaaagct
     7141 tacgccgctc aaatacagtt gcttctcctt cgctagatag gcgaatatat tcaatcaaag
     7201 actcgatcga taatccggca tttcgcatac agatcgtaaa gtcgacccat tttaggtctt
     7261 cttctgtata atcacggatt ccgccttttg tccgattaac tggtggaatc aacccgattc
     7321 gttcataata gcgcaatgta tcggccgaaa tgcccttttc ttcactgact tgtttgatat
     7381 tcatctgatc actctttctc ttaatttctt gccactttac ttatgtattt tatttattct
     7441 acaactttaa gtgtactcca agtcaatact catttctatt aaaactaagc tggattatct
     7501 atatcggttg tttgtgtatt atttcaaata aaaaagtctt gtttttttat tcttttttgg
     7561 atatactttt aacaatttag ggaaatttta ggaaaagggg tataaaaatg acaacgtatc
     7621 agcgaaaggt attagtatcg acatcggcgg gaattgcttt agagaatatg gatattatgt
     7681 ttttagcttt ttcattatct tcaatgatta cttcttttca tattagtggt acgcaagcgg
     7741 gtttcatttc aacgatcact aatttgggga tgctcattgg aggtattttt tttggcatca
     7801 tagctgataa atatggacgt gtgaaagtat tttctcaaac ggtccttctg ttttcgattg
     7861 cttctttatt gatgtatttt gcttcaaaca tctacttggt ttatctcttt cgctttatcg
     7921 caggtgtagg agcgggagga gaatatgggg cgtgtatgtc cttgatttcc gaaagttttt
     7981 caaaaaaaca aattggacga gcttcttcgg ttgctggtat tggtgcgcag gtcggtgcgg
     8041 ctttggctgc gattttagct gctgtgatca ttccaacgtt tggttggaag atgttgtatg
     8101 tcgtaggtgt actgccggtg ttgatggtct tgttcattcg tcgcggcttg actgaaccaa
     8161 aggaattcaa atctgtacaa tctgccgaaa agaaaaccaa acttagcaga ctgtttgaat
     8221 caaaacatct tgcttggcaa acatgtggcc ttagtatcat ggtaactgtc caaattgccg
     8281 gctattttgg gttaatgaat tggctacctt caattatgca aaatcgatta gggctttcgg
     8341 tttctgggtc ttcattgtgg atggtcagta ctattttagg catgtctttg gggatgatga
     8401 cttttggcat aatcatggat aaattggggc ctaaactagc cttttctttg tttttaattt
     8461 gttcatcctt atctgtctat ctacttgtgt tagcccatag ccagtggagt ttggtagcag
     8521 ccgctgtgat agtcggctac tttatcaatg gcatgtatgg tggatatggc gcagtgatta
     8581 gtagtttgta tccaacagaa atccgcgcga cggcgaataa tttcgttatg aaccttggac
     8641 gagctgttgg tggtttttct tctatcgtca tcggtttctt gatggatcat tatagtttaa
     8701 ctgccgtaat cttgtttttg agcggtattt acctagtcag tctgctggtc ttgacaactc
     8761 ttactggtgt tacagcatta agggaaagct tgcagcattg atagttgagt ctgtaaatga
     8821 caagaaagag gtgtggaaaa agcttgcatc tgcggtaata agaccagcca aatcaaaaac
     8881 atgagacact gtttccgttt ggctggtcgt attacttgat gcagggcagc tttttcaact
     8941 gctaatttcc ttctatctgg cggcgactat tctttgacgt ccgtcatttc aaagtacttt
     9001 actaataact tagcataacg aaaaactgtg gatacactcg tatattgagc gtctgcataa
     9061 tcttccaaat ttttctcagg atgttgatac atgaattcaa tcaaatgata attatatgat
     9121 tttctgtaga aatgaagata aataatgtag attgggaagt tctcctgctt ttccatagaa
     9181 aagcgccgat tattctcatt cttcaaaatg accttatcag gatattgttg gaacaattct
     9241 tctaaatctg ttagataatt caagatagac ttacttgata ccttcaattc gttttgtagt
     9301 tcttctattg aacaccaacg ttctgtttca agcagtaaat gaatcatttt aattttcttt
     9361 tgagaaactt ttgttaaaaa agtctgcata ttttctcctc ctgtatctac tttactatac
     9421 tacatacaag aaaaaaacgg atacaaatat catattgtca caccgtcaat agtgcataaa
     9481 cctgttatta tttctctttt tttcctaagt attctattga tatgaatcat ttaatcgttt
     9541 attatataaa tgtcaataat ctaataataa aataacgtac atattttatt attgcatact
     9601 taataacaga actatagcaa aaaatcccgt gtatcaacac gggattagat taagtttcct
     9661 caatcactat tcataacaca attattataa caagtaagta aacaattgaa aaggacattt
     9721 attgaaaacg gttgtttttt acttaaggag gaaatttagt gaaaaaatta gttgcgttgt
     9781 ttttggtcgc aggaagtttg ttgagtgggt gtggtgctac tacgtctact actcaaagca
     9841 caagttcagc tcaggcttcc agcagcagta ccacgatctc cgaaagtacg gcagattcat
     9901 cgaaagaaca aattgaaaag aaaaaagaag aagagaaaca aaaagcggaa aaagctgcac
     9961 tagaaaaaaa agtgacagaa gcggaatctg ctttgtcaca agctgaagca aatcctacaa
    10021 aagaaaacta tgcaactgct tcagcagcta tccaagcgct acctgaaaac aaacaagagt
    10081 tgacaagccg tcttgctgca attgattcca caatcaaagc aaaagaagca gaagaagaac
    10141 ggaaaaagca agaagaaatt gcacgacaac aagcagaaca agcgcaacaa gaagcaatcg
    10201 cacagcaaca agcagaacag gcgaggcaag aagcaatcgc acagcaacaa gcagaacagg
    10261 cgcgacagca agcagcagct gaacaggctt ctcagcaggc acaagccgca gctcctgagc
    10321 aaaataatga acaaactgtc tatgtcacac cgaccggttc aaaataccat acacataaat
    10381 gtggaaatgg tacgtatacc cctgccactt taagcgaagc tcaagctcgt ggtttaaccc
    10441 cttgctccaa atgttatgga aactaattca aaagttatac tataataata aaattcttct
    10501 gaaacataaa aaaatagaag ctggcgttcc agatagtaaa ccaaaaatag cagtttacac
    10561 gtccatttcg tcagcttctc tcttttttca tttagtctgt tccctctgct tttttggaca
    10621 tcttcattca ttctttctca atcaaatagt acacctcttt ttcttttcgc agtttatatc
    10681 ttcctttttt caatttcatt gaaaactgtt tcgaatgttt atgttttaca tggattttaa
    10741 gcaggagctc ctcatgttgc aagcgatagt tcctgccttt taaatcagaa aatctcacta
    10801 gtccgtattc atcagaagct ttcacaaatg gaactttttc tctagtaata tagtgtttgc
    10861 cgttgagcgt ttttacagaa aagaccattc cttcgattgg tcgtccgttt gtgttcttta
    10921 cataaaaaga taaaggatag tttcgcttag cgatggccaa agaatctaaa taagcgatga
    10981 gtagccaaac taagccgatt gatccaagta gtataccgat aaaccaaata aaaaatttcc
    11041 attttttcca ttgatcaatt ccttggatcg ctttttttgc ctcttttgct tgataaggaa
    11101 tacgatgccc cgtcacaagc agtcgatgtg tattgaccat ataaggagtg caggtaagca
    11161 aagtcacgag atctctgcct ttttggatac ccaaagaatc tacttcgtca ggtaagacca
    11221 cttggatttt ttctacttga tacgccaatg tctttccatt gatctggatg aaaaactggt
    11281 ctcctttttt caacttaggc aaatctgtaa ataattttgc ttctgggagt ccacgatgcc
    11341 cagaaagaac ggcatgggtg tttttcccgc cagtcggata agaacttcct tccaataatg
    11401 acgttccttt ttgcaaaaat atttccgttg tctgatcgaa tataggtaag ctgacattga
    11461 tttttgggat ttcgatgact ccaagcatat gttccataaa aaaggcatta ctaggtaaat
    11521 cttttaatgc ttttgcatca actgcttggt tgaaactgcc tatccctggt gatacgtttt
    11581 gctctgccaa ctgttggttt ttctttgtca ttttttcctg ctgttttttt atttcctctg
    11641 aattcttatt cgatgcttgc ttttgataat ggatgatcat ttgctgggca agaaaatcat
    11701 tcagtgaatc ttctacaaaa ggataagaaa agatcccgat cccaacgact aaaatgattg
    11761 ccatgaagat gtccagtatt cttattctgc ttttattcat catgtccacc tattttctaa
    11821 tatgtatatg aagaggttgt gaaatcagcg ccttgacctg agtattagcg aagcaaacgg
    11881 caaaatagtt cctcctattt tgccgtttgt tgagcgaata ccaagagacc gaccacttgt
    11941 cacaaccttt ctccccattt gaatcactat cgtttattta tcgtatgatt tttgtttcca
    12001 atagtttaag cttctgtttc ttgatggcgt tttttgaaat agatcactgc tcctccaact
    12061 aaagcagtac ctaccaagat gaatacgatg atccccatac caccagttga aggcaagatt
    12121 cctttaggtt ggttatcgat cgttgtttca tcagaagttg ttggatcatc atctactgta
    12181 aattcagtgt acgcagcttc attcttaggt aatgcgtagc cttctggtgc ttttgtttct
    12241 tgaaggcagt atgccccttg tttcagccct tcgattgaaa atttcccttc tgaatcagaa
    12301 atcaatttca cttcttccca gtcagaacct gtgacttcat ctgaccaagc atatgggtat
    12361 tctcctgagg caaggtttgc tatttttttc attaatttaa cagttggatc ttttgctctt
    12421 agaatagaaa attctgcatc ggctaatttt tcttgcgtgt tcacatcttg tttgatgaag
    12481 tggaaatcac cgtagttttg gacattttct acagtaatcg tgttatccgt agttgtattt
    12541 tcccgattga ctacaccgtt tggattccca agattggctt catctgcgcc atggatgata
    12601 ccggttgttt gttgcaattt tccgtttttt actataaaag tagtttcttt ttgtggattg
    12661 gttaattgat aaccatctgg tgcttttgtt tcaactaaag tataattgcc gtctattaat
    12721 ccattgacac tgatggatcc ttcagtatca gttgtgaatg tcaacgctga ctcagcagga
    12781 tcatagagag cgacaccagt gttttcatca aaaccggtca cgaattgatt ctctgaattt
    12841 ttgatctgga attgcgcccc tgctaactta tctacccctg aaaagttccc agctttctgg
    12901 attttgatat tgctttcttt gatgacattt ttagcatata ggtgaatcgt gtctaaaggt
    12961 ttgtcagggt ctagggtaag cttaacacga tatttggcga cgtttcgaca ccgggctttg
    13021 gtgtttctac aaagacgtat acagcatctt tcccatcaga tttacctgga agagtcaggt
    13081 tagctaatcc ttcaccagaa gtcgttacac ttgcgaaggg tgtagcgtct gctggaactg
    13141 ctgtgtcttt acttggatag tacgtagcag tgatgtcata tacgttgaat tcaacgccgt
    13201 tcaacggttc gctattcgga aatgtcaatt ccgttccgtt gtttatttga ttttctggtg
    13261 ctgaagtgaa tttcaattta tgcaaattaa ccgtaacatt ttctggtgta ggtgatacgg
    13321 ctgcagaaac ctctgcagtt cctaggccga gaacgaaagg aagcagcatg atagcagttc
    13381 ctaaaaacgt ccatattttt gttttgatgt ttttcatttt atttccctcc tattgtttcc
    13441 tttttgttgt tttttataaa accaagctgt ccctgttgaa aggactagca ataaccctaa
    13501 aattgagaaa cctccaagca tctcttcgct tgtttttgga aagcttcctc catttttttc
    13561 atttgatgga ccatttgtct gcatctgaac tggcgttttt ccattattcg tcggatttgc
    13621 ttttggttcg ttgatgattt ccaatggtat aggatgaagc ggatcataac tgcctttttc
    13681 aacttcaaag agaatggttg attgactcaa tatatatcct ttaggcggtt taatctccac
    13741 tagttcgtaa cttccaaaag acaatccatg gacagaaaat gttccatctt tactggaagt
    13801 aaaaatcgtt gctcttgaat catctttggc tacccaatga tttatcccgt tttcttgcgc
    13861 taagtattgg tcattttttt tgactagaaa ttgagcatct gctagttttt gtttcgtttg
    13921 ccgatccact ttgacaaatt gtttgcctcc tgtttggatg gcaagatgtg tttctactgt
    13981 ttgattgtca gtcgtcaaac gtacatcgtt atcaaatgtc gttttttcac tgttttcttt
    14041 caatgtcatt tggtaacgga tcgttaattt ttttccagca aacgaactaa gatcttttcc
    14101 agaatctgat aagacaaaac catgttttat tttttgtgtg tgaaatgtat gaatcttgtc
    14161 tccaccaatc aaaaaatcga tcgtttctgg ttctagccat aactgaggat cagctgtatc
    14221 tttgatcgta taggttccta tctcgttgat gttctcagga attgttacgg tgatgagata
    14281 agggacttta tccccatctg caaaatctgt ctgcttattc tctactgttt ttgttaatgt
    14341 gagtgcagca ggtatttttt gttcgctctt tggatacaag tggatggtcg tcaatttatg
    14401 attagatgaa tctgtcacag gaagcacaac gacaaatggt gctgattttt cttttactta
    14461 atctgaagtc tttatttccg taaatcggta aacagcgtca cgaccttgct catctttgtc
    14521 cgatgcacta aaagaagcaa tgccctcttc ctcgtctact gtttttgtca cattttctgc
    14581 cagtattttt tcactatcac ttttttgtgc tagttttctt tgtgcgtctt ccacactact
    14641 tccttctgac cgcagcttgt aaaattcatc cgagacatca tatatagcga aagtaacgcc
    14701 gttcaaccct tttactcctt gaaagttatc cgggatattt attccgtcgt tttgactgat
    14761 acttggcatc ttatcgcttt cgaacattcg attatgcaaa gtaaatgtaa taggtgattc
    14821 tctagctgac acagtcggag aacctataaa aaagaccagt aggataaaaa agagaacagc
    14881 aaatatatcc acacgtacat tttttgtttt tttcatcgct gttcacctgc ttttttctta
    14941 cgatggataa aataaaagaa gccagaaaaa ataaagatca gtcctaaaaa gacaaagggc
    15001 agtagaccta tcccacctgt cgaaggaagc aagagagtcg gattgatcgt caacttagcg
    15061 gtatctgttg cgataatcgt ataattattg tttacacttc cttcgcctaa actaatcgtt
    15121 actatttgtg atccaatatc tgtccggctg ttaactgtta gggtataatc caatgattcg
    15181 cctaagacaa caccggtaac gtggagattt ccaaccgaaa tcgagtgact ttgggcatca
    15241 taattaaagg cagcgtcatc gacagtaatc actgcgttag ctttatagat ggtataagtt
    15301 cctgatgtgt tggctatatc agtaaatgac aacttgggat ttgctgcttt gactttgtct
    15361 attccgctat tcgttagttt aattgtatag ctgccagcat taaccatatt ttttgctgat
    15421 tgtgcatctg ctgtgtaatc catactgcta agtgccactt gtactggagt aagcgaacta
    15481 gtgacaccct ctgctagctt gacatggatt gacgggacac ctgcttttcc atcatatgtc
    15541 ttactgccac tgagctctac cgcaccgctg atttgcttaa tcgtatagac atcagatgtc
    15601 tgcacgatag taaataaata gtttgttctg gcagctagtt tagctagtcc gctttttgtc
    15661 aattgccaag acgtataggt tccaatttca gtaggcgcag ttacttcaac tcccttttca
    15721 ttcatcgctt tgaaatcagt tggattccat gtgatttgat caatactgag atcattgttt
    15781 aaaagggatg ttgattctgt tttatcatta aaggcatatt taacgatcgg tacaacagaa
    15841 agaccatcat agtacttact gccatttgca ttgattgata ctgttgcatt tgtcttataa
    15901 tataggatga cttcagaaat ctgttttcca tttgcgtcct ttacgattac atttccattt
    15961 gtatctaaac tagctaaaac actcattgct gattggttct ttgtttctat cgctgaaaca
    16021 gctgtaaagg tatatccgtt gatagtaggc gtattagtcg tcaggtcttg tgcagcactt
    16081 ccgtaagtaa gattcattat tttagaagga gcaatcatct gtccaaaata gtcttgatac
    16141 ttaatagtga ttggtgtttg tttgcctgta taggtcaccg tatcgacgtt actttctagg
    16201 gctgtgaaag aaccgtttgt ttgtgcaaaa gaatagcccg ttactgctgt tggagtgata
    16261 accactttgt cacccacatg gattccagat aatgtagtcc cgtacgttcc gatttttgct
    16321 gctccaccat tgactgtata agaaaaagtt cctgtccgat tcgcattgac ctgaaccgtt
    16381 gctgcttgat aggttggaaa atttgaaaca tttcccttac catcaacgat ctgcccattt
    16441 ttgatcgagt accccgtgaa tggaatatca tttgctaact gtataccgat tgaaaaagta
    16501 ctcatacctc ctccggcagg aagccctccc catgttgagg gtgtaaaaga ataactttta
    16561 tccgcattgg gtgtaactga atcgatcgta tttccagcgg tagccacccc catatagata
    16621 ctttttggga atgtgatagt agtaccattt actgtggagc caataaacag attatggata
    16681 tctggacggt tatcaataaa agcgattgct ttggttacgc cttgtaacgc agttttatcc
    16741 gcactcatcg atccgttaac cgcaacaatg actccatctt tggcatatat cactgtatat
    16801 ccatctggta tggtatgtcc gttgaaattc gcagtggccg caacagtact aaatgtctgg
    16861 cttctacctg tttctgaata agctttctcc cctgctgctt ttgctgctgt gatttctttt
    16921 tctgtcggat caatcagttg agtcgaggat gtggttgagg ttgtctcgtt gttgctagac
    16981 tgattggtgc tttctatgga ctgtatttca gaatagggtg gactatttgt ctcctctgtc
    17041 gtttggttag caaaatttga cgtttctgta taaacgttta cataaatact tgataagctc
    17101 cctaagaaag taaaaatcaa gatgatcgaa atcataatgg gatgtaagta ttttctttta
    17161 gatacagctt tgagtctaga aacaaaatgt ttttgacata tacgttggat tttattttga
    17221 gttttcttaa tcattttcat ccctcctctc a
//
LOCUS       DACDRK010000053        17131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDRK010000053 DACDRK010000000
VERSION     DACDRK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.053"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..125)
                     /locus_tag="IWJ77_001901"
     CDS             complement(<1..125)
                     /locus_tag="IWJ77_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9453498.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(229..540)
                     /locus_tag="IWJ77_001902"
     CDS             complement(229..540)
                     /locus_tag="IWJ77_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9453499.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(542..739)
                     /locus_tag="IWJ77_001903"
     CDS             complement(542..739)
                     /locus_tag="IWJ77_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9453500.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1133..2860)
                     /locus_tag="IWJ77_001904"
     CDS             complement(1133..2860)
                     /locus_tag="IWJ77_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9453501.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2853..4046)
                     /locus_tag="IWJ77_001905"
     CDS             complement(2853..4046)
                     /locus_tag="IWJ77_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9453502.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4233..4877)
                     /locus_tag="IWJ77_001906"
     CDS             complement(4233..4877)
                     /locus_tag="IWJ77_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9453503.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4971..5105)
                     /locus_tag="IWJ77_001907"
     CDS             complement(4971..5105)
                     /locus_tag="IWJ77_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9453504.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IWJ77_001908"
     CDS             complement(5107..7239)
                     /gene="feoB"
                     /locus_tag="IWJ77_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9453505.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7236..7709)
                     /locus_tag="IWJ77_001909"
     CDS             complement(7236..7709)
                     /locus_tag="IWJ77_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9453506.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8110..8334
                     /gene="nrdH"
                     /locus_tag="IWJ77_001910"
     CDS             8110..8334
                     /gene="nrdH"
                     /locus_tag="IWJ77_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9453507.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8493..10652
                     /gene="nrdE"
                     /locus_tag="IWJ77_001911"
     CDS             8493..10652
                     /gene="nrdE"
                     /locus_tag="IWJ77_001911"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9453508.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10702..11667
                     /gene="nrdF"
                     /locus_tag="IWJ77_001912"
     CDS             10702..11667
                     /gene="nrdF"
                     /locus_tag="IWJ77_001912"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9453509.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11756..12895)
                     /locus_tag="IWJ77_001913"
     CDS             complement(11756..12895)
                     /locus_tag="IWJ77_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9453510.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13204..13917)
                     /locus_tag="IWJ77_001914"
     CDS             complement(13204..13917)
                     /locus_tag="IWJ77_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9453511.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IWJ77_001915"
     CDS             complement(14171..14872)
                     /gene="nagB"
                     /locus_tag="IWJ77_001915"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9453512.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15106..16638
                     /locus_tag="IWJ77_001916"
     CDS             15106..16638
                     /locus_tag="IWJ77_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9453513.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16829..17008)
                     /locus_tag="IWJ77_001917"
                     /pseudo
     CDS             complement(16829..17008)
                     /locus_tag="IWJ77_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(17028..>17131)
                     /locus_tag="IWJ77_001918"
     CDS             complement(17028..>17131)
                     /locus_tag="IWJ77_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9453514.1"
                     /translation="LESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         5316 a         3523 c         2929 g         5363 t
ORIGIN      
        1 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
       61 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
      121 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
      181 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
      241 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
      301 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
      361 aaaatataga taattcacca atctatttat taaaggactg ggatctaatt catatttttg
      421 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
      481 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
      541 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
      601 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
      661 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
      721 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
      781 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
      841 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
      901 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
      961 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttgaacgt ttcgaagatg
     1021 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
     1081 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
     1141 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
     1201 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
     1261 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
     1321 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
     1381 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
     1441 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
     1501 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
     1561 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
     1621 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
     1681 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
     1741 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     1801 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     1861 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     1921 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     1981 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     2041 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     2101 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     2161 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     2221 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     2281 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     2341 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     2401 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     2461 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     2521 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     2581 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     2641 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     2701 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     2761 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     2821 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     2881 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     2941 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     3001 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     3061 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     3121 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     3181 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     3241 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     3301 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     3361 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     3421 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     3481 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     3541 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     3601 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     3661 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     3721 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     3781 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     3841 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     3901 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     3961 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     4021 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     4081 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     4141 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     4201 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     4261 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     4321 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     4381 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     4441 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     4501 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     4561 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
     4621 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
     4681 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
     4741 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
     4801 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
     4861 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
     4921 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
     4981 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
     5041 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
     5101 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
     5161 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
     5221 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
     5281 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
     5341 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
     5401 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
     5461 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
     5521 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
     5581 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
     5641 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
     5701 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
     5761 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
     5821 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
     5881 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
     5941 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
     6001 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
     6061 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
     6121 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
     6181 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
     6241 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
     6301 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
     6361 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
     6421 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
     6481 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
     6541 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
     6601 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
     6661 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
     6721 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
     6781 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
     6841 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
     6901 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
     6961 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
     7021 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
     7081 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
     7141 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
     7201 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
     7261 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
     7321 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
     7381 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
     7441 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
     7501 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
     7561 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
     7621 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
     7681 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
     7741 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
     7801 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
     7861 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
     7921 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
     7981 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
     8041 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
     8101 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
     8161 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
     8221 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
     8281 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
     8341 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
     8401 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
     8461 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
     8521 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
     8581 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
     8641 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
     8701 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
     8761 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
     8821 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
     8881 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
     8941 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     9001 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     9061 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     9121 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     9181 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     9241 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     9301 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     9361 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     9421 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     9481 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     9541 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     9601 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     9661 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     9721 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     9781 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     9841 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     9901 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     9961 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    10021 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    10081 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    10141 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    10201 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    10261 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    10321 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    10381 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
    10441 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    10501 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    10561 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    10621 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    10681 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    10741 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    10801 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    10861 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    10921 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    10981 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    11041 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    11101 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    11161 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    11221 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    11281 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    11341 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    11401 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    11461 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    11521 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    11581 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    11641 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    11701 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    11761 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    11821 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    11881 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    11941 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    12001 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    12061 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    12121 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    12181 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    12241 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    12301 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    12361 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    12421 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    12481 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    12541 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    12601 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    12661 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    12721 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
    12781 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    12841 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    12901 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    12961 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    13021 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    13081 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    13141 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    13201 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    13261 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    13321 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    13381 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    13441 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    13501 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    13561 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    13621 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    13681 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    13741 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    13801 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    13861 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    13921 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    13981 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    14041 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    14101 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    14161 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    14221 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    14281 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    14341 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    14401 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    14461 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    14521 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    14581 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    14641 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    14701 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    14761 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    14821 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    14881 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    14941 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    15001 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    15061 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    15121 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    15181 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    15241 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    15301 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    15361 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    15421 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    15481 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    15541 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    15601 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    15661 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    15721 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    15781 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    15841 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    15901 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    15961 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    16021 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    16081 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    16141 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    16201 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    16261 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    16321 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    16381 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    16441 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    16501 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    16561 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    16621 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    16681 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    16741 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    16801 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    16861 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    16921 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    16981 caataaagta cataaacata aatacagacc cgtggtgcta gttaatttta atatcctaaa
    17041 gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac actgtaagcc
    17101 agtaatatgc tttcaaatct actctctagc c
//
LOCUS       DACDRK010000054        16188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDRK010000054 DACDRK010000000
VERSION     DACDRK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.054"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            141..1478
                     /locus_tag="IWJ77_001919"
     CDS             141..1478
                     /locus_tag="IWJ77_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9453515.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1526..3655
                     /locus_tag="IWJ77_001920"
     CDS             1526..3655
                     /locus_tag="IWJ77_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9453516.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3630..4319
                     /locus_tag="IWJ77_001921"
     CDS             3630..4319
                     /locus_tag="IWJ77_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453517.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4727..5413)
                     /locus_tag="IWJ77_001922"
                     /pseudo
     CDS             complement(4727..5413)
                     /locus_tag="IWJ77_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5549..5743)
                     /locus_tag="IWJ77_001923"
     CDS             complement(5549..5743)
                     /locus_tag="IWJ77_001923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453518.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5793..6233)
                     /locus_tag="IWJ77_001924"
     CDS             complement(5793..6233)
                     /locus_tag="IWJ77_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9453519.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6237..7082)
                     /locus_tag="IWJ77_001925"
     CDS             complement(6237..7082)
                     /locus_tag="IWJ77_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9453520.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7231..8649
                     /gene="pepV"
                     /locus_tag="IWJ77_001926"
     CDS             7231..8649
                     /gene="pepV"
                     /locus_tag="IWJ77_001926"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9453521.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8706..9653)
                     /locus_tag="IWJ77_001927"
     CDS             complement(8706..9653)
                     /locus_tag="IWJ77_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9453522.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9876..10226)
                     /locus_tag="IWJ77_001928"
     CDS             complement(9876..10226)
                     /locus_tag="IWJ77_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9453523.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10426..11505
                     /gene="pepA"
                     /locus_tag="IWJ77_001929"
     CDS             10426..11505
                     /gene="pepA"
                     /locus_tag="IWJ77_001929"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9453524.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11649..11969
                     /locus_tag="IWJ77_001930"
     CDS             11649..11969
                     /locus_tag="IWJ77_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9453525.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11991..12455
                     /locus_tag="IWJ77_001931"
     CDS             11991..12455
                     /locus_tag="IWJ77_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9453526.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12660..13265
                     /locus_tag="IWJ77_001932"
     CDS             12660..13265
                     /locus_tag="IWJ77_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9453527.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13304..14593)
                     /locus_tag="IWJ77_001933"
     CDS             complement(13304..14593)
                     /locus_tag="IWJ77_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9453528.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15021..15500
                     /gene="rlmH"
                     /locus_tag="IWJ77_001934"
     CDS             15021..15500
                     /gene="rlmH"
                     /locus_tag="IWJ77_001934"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9453529.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15703..16188
                     /locus_tag="IWJ77_001935"
                     /pseudo
     CDS             15703..16188
                     /locus_tag="IWJ77_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5122 a         2965 c         3289 g         4812 t
ORIGIN      
        1 tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct ttaatgaaag
       61 cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta tcagaaaaac
      121 aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta gttatctcat
      181 tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc tttgccatca
      241 tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca aacccggcga
      301 tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg ccagctgagt
      361 acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt gttctagtag
      421 atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg gacaagccaa
      481 gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg atgacaggaa
      541 tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca ttaggtattt
      601 cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc tttgctaata
      661 tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg gctgaaagct
      721 atacttataa tgactatttc caaattggta tgattgggat gattgtaagc ttagttgtgg
      781 tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg gcagcaaacg
      841 taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta ttaccagttt
      901 taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca ggtatctggg
      961 cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt cagtttgcga
     1021 aattatttac agatggcgca gtagatgttg cgccaatggt tggtttcttg atgaccttag
     1081 cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt tttggagatt
     1141 ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca ccactaggat
     1201 ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct gtcgtattag
     1261 ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc acaacgattg
     1321 ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt tatacaaaag
     1381 taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc ggagcgatct
     1441 tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt cgataaaaac
     1501 gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa ttgatgttgg
     1561 cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca tcggaaaatc
     1621 ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg ttcagtcttt
     1681 tcaaaattgt ttgctggaaa ataatatcag tccagaagat gttgtgtttg ttgcacattc
     1741 taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg gcgtaatcgg
     1801 gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat tgaacgatat
     1861 cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg tcaaacactt
     1921 aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag cggaggtctt
     1981 ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg tttatgaagc
     2041 agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat tatatggatt
     2101 gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa tgctagatac
     2161 ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt tgatgattat
     2221 gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg ttttaaccat
     2281 gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag cgtctaatgg
     2341 tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa atggacgtcc
     2401 agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat tagatgttcg
     2461 ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa tcatcgatgt
     2521 cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg agacagaaaa
     2581 aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg ctgattacgt
     2641 caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg cagccaatgt
     2701 ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg cacgaaaagc
     2761 catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag aacagatcat
     2821 ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca agtatcactt
     2881 ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat tgattactta
     2941 ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag tgatttcttc
     3001 tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc cttcgccaag
     3061 taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg aatcaggcgc
     3121 tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa aagtaacggc
     3181 tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga tcacaacaga
     3241 agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag tttgcttatt
     3301 ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa atgctggaag
     3361 tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg cttcatgtat
     3421 gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag atatggcagt
     3481 ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag caagagtctc
     3541 tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg aagtatctac
     3601 tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa cataagttag
     3661 aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc taccagtttc
     3721 ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt atacaacaat
     3781 caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct tttgccgatg
     3841 taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca gagcaaataa
     3901 acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg atgacggagc
     3961 cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca gcgcaagctt
     4021 tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga atttatacgc
     4081 aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca aacattcgtg
     4141 cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc gatttttcgc
     4201 cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc caagggatct
     4261 tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat tacaaataaa
     4321 gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacggt gttctcctgt
     4381 caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt tttgaaattg
     4441 ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg gtgttcaaaa
     4501 gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta tttttacttg
     4561 gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa acggtgtgca
     4621 atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa ctattttgcc
     4681 gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat ttgatgggtc
     4741 tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct tgctcctcga
     4801 taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta tgatcaatca
     4861 ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat tctgaaatcg
     4921 tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc caatcccaaa
     4981 tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc aaatcaaaaa
     5041 aagctttttt attttgagcg attactgcct gatcggtttg ttctagtatt ttttctagtt
     5101 tagcttgatc ttacttggtc attttcgcac tgcattcttg caggatcggt ttttccaact
     5161 tttcccgcgt ataacgggct aatgaagcag atcggatatc gatttttgaa acataagtgc
     5221 cggactgtgg aatggttttt acaagctctt gattgcgcag ttggatcagt gcttctcgaa
     5281 tcggtgtacg cccgatagct agttgttctt ctaacacttt atcggagatt ttacttccag
     5341 gtgctaactc ggaataaatg atttgtttac gtatagattc ataagcttgc tgttgcaaat
     5401 tattgataac catttaacct tcctttctat gcagatataa tctattataa cataaatcgc
     5461 ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa agccctgata ggcatgccta
     5521 ttagagcctc gaaaagaaaa atattgaatc aatctggtcg tacctcttgg atcggtttga
     5581 cacgaccgcc gattctttta gcaaaagctt cggattcttt tttgtctttg aaaatgagca
     5641 gattgtctaa gtttttttcc cgagtgcgac agccagtttt atcgatgtaa ccgtgcatta
     5701 ctttaacgac atacatgggg atcacctgct tttttctttt cattatacga actactcgac
     5761 acaatacaag aaaaatgctt agacggtctt tattaagaat ttacatcatc caaagaagcg
     5821 tacgcttgga ataactgatc taactccgga ttgactttgc tcagcttcac taatcgttgc
     5881 gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag tttttaattt tttgtcttca
     5941 gttcccacaa tttcgtagct aaaatccaac cgtgtccccg tatacttttg gatagttgga
     6001 tggatgctca ctttttctcc gtaatggatc atttctttat agctgcaatt tacttccaga
     6061 acggggatga tgatcccaga ttcttcgatt tttttatagg gaaaccctag atgtaccaat
     6121 aaggctaccc gagcctcttc aaaccagcgg atataattgc tgtgatgaat aatccccatt
     6181 tgatctgttt cataataaaa aggttctcgc acataacctg taaatttctc catgactcat
     6241 gcctcgtttt ccattgattt tttacaaaaa taattcatcc atttcggaag tgcttcgctg
     6301 atttgtgtcg gcaaaacaat atattgttct tttgccagct ggtctccaat gaaactatga
     6361 agataaatag ctgctgcaat cgtttggtca ttttgaggaa actgggctaa aaatcctgtg
     6421 atcatcccag ctaaagtgtc acccatgccg cccgttgcca tagcagcaga acctaaagga
     6481 ttggtgtaga agtgctggtg accataaatt tttgtctcat gacttttcaa aacgagcaca
     6541 gcaccgattt cctgttgctt ctttttgttt tctttatccc gttgttcttg tatcggtaga
     6601 gaactcaacc gttgccactc catttgatga ggtgtaagta cagtttgttc gggatagggt
     6661 agcaaaagat gattagcagc aagtaaactt aaagcagagc catcaatcac cagccattgt
     6721 tcttttgatt ggttttctag tacttgtttg aaacgttcca aactcttaga agaagtaccc
     6781 aatccgggac caatcaagag tacatctgct tgctttatta cggaaagaaa ctggtcagta
     6841 tccgtccaaa ctacaatcat tgcttctggc agatgactgt gaagagcact ccgatttttt
     6901 tcatcagtta ctacagtcgt gagacccgct ccgctgtgta cggcagctaa agcactcata
     6961 atgattgctc cgccatattg ttcattgccg ccaatcaaaa cgatacgtcc aaatgttcct
     7021 ttatgtgaaa tttttggacg aggtgtgatg actcttttca ctagttgttc gtttatcagc
     7081 atctaaaaac ctccttttac tcattatagc gtatattttt tttagagaaa aacagataag
     7141 aaaaggaatt gtcagatttt ttgaactatt tttttctgta atttgtggta tcatgaacac
     7201 gaagacttaa ataagtggag gattaacttt atgacaattg attggcaaaa agaagtagaa
     7261 gcacgtaaag aagacttgct tgaagacttg aaaaatcttt tacgtgtcaa cagcgagcgc
     7321 gatgactcaa aagtaacacc agatgcacca tttggacctg gacctcgcga cgcattgaaa
     7381 catatgttag cttatggcga acgtgatgga tttaaagtaa aaaatgtcga taactatgca
     7441 gggcatattg atcttggcga aggagacgaa acgctaggga tatttggaca catggatgtc
     7501 gttcctgcag gagatggctg ggacacagat ccttatgaac cagtaatcaa ggatggaaag
     7561 atctttgcac gcggatcaag cgatgacaaa ggtccaagta tggcagctta ctatgcgatg
     7621 aagatcatta aagaacttga cttgaaacta tcaaaaaaag ttcgcttcgt agtcggcagc
     7681 gatgaagaaa gcggctgggg cgatatggct tattatttcg aacatgaaga agaaccagat
     7741 tttggtttct caccagatgc tgaattccca atcatcaatg gtgaaaaagg aaatgtttct
     7801 ttagctttac gcttcaaagg cgacaatgca ggagattatg tcttgaaatc ttttgtttcc
     7861 ggacttcgcg aaaatatggt gccaggaaca gcaactgcag cacttcaagt accaagtgca
     7921 gatgcagcta tcgcgatgga agaagcattt taccaattca tcgaagcaaa tccagtaagc
     7981 ggaacgattg aagcggacaa tacgtatgta aaaatcgaat tagttggtaa aggcgcacat
     8041 ggtgcaagcc cacaatctgg tatcaatgca ggttctttct tggcattgtt cttagataac
     8101 tatgaattcc taggatctgc taaacaattt atccatgtag cagctgctta tgtccatgaa
     8161 gatttttatg gagaaaaact aggagtcgct tatgaagatg aaaaaatggg caaattgaca
     8221 atgaatgctg gactatttgc ttttgaagaa aatggtacag aggaagcgaa cttcatcaat
     8281 atgaatttcc gttttccaaa aggtgtaaca gtagacggat tgcaaagtga catcgaacaa
     8341 acagtggggc aagaaggcgc aacagttact cgaggtgctc gcgtaatgga accacattac
     8401 gtaccaatgg atgatccatt ggtggctaca ttattgcaag tctatgaaga ccacactgga
     8461 gaaaaaggct acgaacaaat catcggtggc ggaacttacg gacgtttgtt gaaacgtggc
     8521 gttgcttatg gagcgatgtt cccaggatat actgatacaa tgcatcaagc aaacgaattt
     8581 atgagtcttg atgacttgtt cagagcaaca gcaatctatg cagatgccat ctatcgtttg
     8641 gcgaaataat taattaaaaa gaggatcaag aattgcaaat tatttttgca gtcttgatcc
     8701 tttttttact ttctactgtg gtattcatga taaacatgat atagattcaa ttcatattct
     8761 ttcttagcgt tcgtgacgac tgtatccaaa atcaaccctg ttactaatga aagaagtgct
     8821 aaagtcatca atccagtact taaaatcgca gaagggatgc gtgtaataaa gcttgtcagc
     8881 atatattcgc gaatcacagg aatccccgtg accaatccaa ataaaaagaa gaagatggtc
     8941 caaatcccaa agaaaagcaa aggcttataa tctttaaaca ttttaataat catcatgatc
     9001 actttgaatc cgtcagagaa tgtattcaat tttgattcgc tgccttcagg acgatcacga
     9061 taatcgattg ggatttcaac gaacttgaat tttttatcta aagcatggat cgtcaattcc
     9121 gtttcgattt gaaaaccaga actcataata ggaaaacttt tgacaaagat acgattgaat
     9181 cctcgatagc cggtcatgac atcaagaatt tttgttttat acattctagt aattgtattt
     9241 ctaacaaggt tatttccaaa atcgtggaaa ggtctcttat tctcatcaaa gtaggttcca
     9301 tttgataggc gatcaccaat gaccatatct gcttcaccag aacgtaattt ctcgagcaaa
     9361 ccatgtacag cttctgcagg ataagtatca tcgccatcta ccattaaata atagtcggca
     9421 tcgatatcaa agaacatttg gcggatcaca tttccttttc cttgacgagg ttcttttttt
     9481 acgattgctc caccttgaac ggccaattcg tatgttcgat cggtcgaatt attatcatag
     9541 acataaatat ctgcttctgg taattctcgt ttaaagtctg caatgactgt tgaaattgtt
     9601 gcttcctcat tgtaacaagg aatcagaaca gcaatttttt cttgttgttc catttttcac
     9661 tctccttaac tatagaaaac aaaagctaaa ttaaaaatac ctcataatca aaggaaatgc
     9721 tagtttttcc cgctaaatat catttattta aaaagaaaat actggaatat atgtttggaa
     9781 agctctatct aaaaaaccac ccactacaaa agaaggacat gatacctctc ttttgaatag
     9841 tgggtgcgta ttcgttctct cacttttttg acgtattaat ctgttgcacc tttttttctt
     9901 gaaatttcta atacggttcc tgtatgagca tctgctaaaa attcataata aacagtttcg
     9961 ccatcttcta ttcgagaaag acctccccga taagcactcg accgaacggc gaatttccta
    10021 ataggttcct tttcaaaaga gatccaagaa ccttcgattg caccttcttt tagaaaagat
    10081 gacttgatat tttcaagtac ttggtctgct gacaatactt ttttgcgatg ataccaaatc
    10141 gttgaagcta ccccgctcaa taatcctaga ccgattccaa ctgctaaccc gcctttaaaa
    10201 tagcttagtt cgctttcttc atacatgtgg acatacctcc ttcttctcac ttgttactcc
    10261 ttgtgcttat tttaccacac tctcatattt tctttgaaat aaataaccgc taccagaaaa
    10321 aataagctat aataaatacg atcggtctgc agaaaaaatc atattttatc cgcagatcaa
    10381 tataagtctg agaggagttt agtctgaaat ttagacgaat agcatatgga agaaaaaaca
    10441 tttcaacgaa tcaaagagtt aacagaatta caaggaacaa gcggttttga acaagatgtt
    10501 cgcgcttata tggaaagtca tatggaaccg ctggtagatg agttacagtt agatggtttg
    10561 ggcggcattt ttggtctgcg ccatcatgaa gaagcggatg caccccgtgt aatggtcgca
    10621 gcacatatgg atgaagtcgg attcatgctg acacaaatcc aagaaaacgg gctttttaaa
    10681 gtcgtacctc taggcggctg gaatccttac gttgtttctg ctcagcgttt tacacttaaa
    10741 acctctactg gtaagaatta tccatgtatt tcttcttccg ttcccccaca tttattgcga
    10801 ggaacaagcg gacaaaaatc agtagaagta accgatattt tgttcgatgc tgggtttgaa
    10861 tcaagagaag aggcaatgag ttacggtgtc cttccaggag acacaatcgt tccttacgct
    10921 gaaacgatca aaacagccaa cggaaagaat attatcagta aatcatggga caaccgctat
    10981 ggctgtacaa tggtattaga agctttagaa gcgttgcaaa atgaaacatt aggtcataca
    11041 ttgattgcag gcgcgaatgt ccaggaagaa gtaggcttgc gtggttctaa agcttctgtc
    11101 aataaattca agccagatct gttctttgca gtagactgct ctgcagcaga tgatacagtg
    11161 acgaaaaatg gcacattcgg acatttagga gaaggtacat tgatgcgtat ccaagatcca
    11221 ggtttgatca tgcttcctcg tttgcgcgag tacttgctgg atatcgctga aacaaacaat
    11281 atcccttatc aatactttgt ttcaaaaggc ggtacagatg caggtgctgc tcatacacaa
    11341 aacgaaggaa tcccaagtac agtgattggc gtagtgggac gttatatcca tacgcatcaa
    11401 acgatgttca gtattcgcga ttttgaagct gcacgagaaa tgttgattca aacactgaaa
    11461 ggtttggata aatcaacagt aaatacgatc gtatacggaa aataatgcga tagtgaaact
    11521 ataaaatctg gcaaaagatt gctttttgcc agattttttt tataaaaaga ggatcaatgt
    11581 ttgttagttc gaaagaaagg tgtataattt aattgaaaaa tacataaaca gaaagtagag
    11641 gacaaagaat gattatccca aatagtttag aagaattagc aggctacgta gaaactggaa
    11701 agagtgtatt tttctttact gccggctggt gcggagactg ccgttttatc aaaccgcaaa
    11761 tgcctgaaat tgaaaatgac ttttctgatt ggcaatttat tgaagtagac cgtgacaagt
    11821 acatagatgt agccgctgaa tggaacattt ttggtatccc aagttttgtc gtgatccaag
    11881 acggaaaaga attaggtcgt ctagtgaata aagatcgtaa aacaaaacag gaaatcgaat
    11941 cgttcctaga aagtgtaaca aaaggctgac atctgttagg aggggtcaag atgaatcaag
    12001 aatataagaa tattttagta ggtatcgacg gtagtgaaca agcacttgct gctttcaaaa
    12061 aagcggtaga agtggctcgc cgtaataatg ggaaagtata tgtagcaaac gtaatcgatc
    12121 agcaatttta taactttatg ggctatgcac ctattgatca aaatctagtc gatcagcaaa
    12181 ctgaaacagc caaggaaatg atcgaagaat gtaaagaata tgggaaatct gttgattaca
    12241 caaatataga aggaattatt gcatatggtt ctgttcgtga atccatggca gtcaaattgc
    12301 caaaaaaata tgagatcgat ctaatcatgg ttgggcagtc aggtctaaat gcagtcgaac
    12361 gctttatgac aggaagtgta gccagctacg tgatacgtca ttcaccttgt gatgtcttga
    12421 tcgtttctga cgaagaagaa cagccaagta attaagcaag tggaacttgt acgacctttt
    12481 taatcaatta actaaaacag taaagggact gtgacaaaag tcttgtcaca gtcccttgtt
    12541 acgaataaat ggtgttccag aagaggcttc ttcggaaata aagcatgaaa aaaatttcat
    12601 gcagtgaatg caattttttg ttaagataaa tctcagcaaa ggataatgga ggaaagaaca
    12661 tgatttttgc ttataacaaa gcacacgtcg gagacacctt gatggttatt atagccgacg
    12721 ataaaggaac agaaaacact gttgaacgca aatggaatgt tgctcggatc aaagatgaac
    12781 aaggtcaagt tgttgcttgg aactttttcc atatctccga tcatctaaca atcgaaggga
    12841 acggccaagt aagcttaaca gatgaggaat tagcgaaatt gaatcgattg atcaaagaag
    12901 caggattcga agaacagctt gaagctgatc atgaaccgaa gatcgttgtc gggtacgtaa
    12961 aaacatgcgt cccacatcct gattctgacc atttatcgat tactgagaca gaagtagaca
    13021 atggtacagt attgcaaatc gtttgcggtg cacccaatat cgaagctgga caaaaagttg
    13081 tcgttgcaaa accaggagct atgatgccag atggaatgat gatctggcca ggaactttgc
    13141 gaggggtaga tagctatgga atgatctgtt ccgctcgtga attgcatttg ccaaatgctc
    13201 cagcaaaaaa aggaatctta gtattgcctg aggatgccgt agtcggagaa gcatttccaa
    13261 aataaagagg ttgtgacaag tctttgtcac aacctctttt ttattaattt tcactatttt
    13321 gattttgttt taatgccgat tgatcgatcg tcaattcaac agtagttgtt ttttctttcg
    13381 agccttcata gtaagtgatt ttgacagaat ctcccacttt tttcttatac aaagcagatt
    13441 gaagttctac accagaagaa acttctttgt catcgatctt agtaatgacg tcgtattgtt
    13501 tcaatccagc tttttcagcc ggtgtagctg tttggacatt tgtcacgatg acaccgttag
    13561 ttacagatga aggaatcttc aagatttgtt cttgttgctg agtagaaacg gcagataagt
    13621 cgaccattgt gatacctaga gctggacgtg tcacttttcc gtctttttcc agttgattga
    13681 tgatattcac tacatcgtta cttggaatag caaatcccat tccttctacg cttacattag
    13741 aagtagattc agaagtactt gcgatcttac ttgagttgat accgataact tgtccttcga
    13801 tattaactaa aggaccacca gaatttccag ggttgattgc tgcatctgtt tggatcgcat
    13861 tgatattgac tgtttcgttc gattcattcg tacttgtcac ttgtcggttc aaggaagaaa
    13921 tgattcctga agtgacagaa tttgcatatt cagatcctag tggggaacca atcgcaatcg
    13981 caggctcacc aaccttcaga gcacttgagt caccaaaaga ggcaactgtt tcgactttgt
    14041 ctgaattgat cttcaacacc gctaaatctg aataagcatc tgttccaact aattcagctt
    14101 ttaccttcgt accgtctttc atcaagactt caagaccttg ctggccatct actacgtgat
    14161 tattcgtgac tacataagca gtattcccgt cttttttgta gatcacgccg cttccttcac
    14221 tataagcttc caaattacta tcatctgagc tgctactttc ttcttgctgc ccgaatagtc
    14281 cgccaaaacc gcttgattgg ttttgacttt gtaagttgat aacagaaacg acagcgtctt
    14341 gaactttatc taccgctttg gtgatatcag aatctacatt gactttgaca ttttccacaa
    14401 ctgtttcacc ggcagaattc tgattgccgc ttgtagcagt agaactattg ttgccagtgc
    14461 ccatcgctgc ataaaaaatg ccagctgtca gtagtcctcc aacgatccct ccaacaagcc
    14521 ccagacctaa cttacgccag aggctattat ttttactttt tttcatcttt ggtgtcacat
    14581 tttttctatc catgtgttcc tcctccaatt acattcattt atctactgtt agtatagtct
    14641 tatttgttga ctttagtcta cttttagact gtgaactttt tatgaaaaac tttaggaaaa
    14701 atcattgttt tcagatgaaa atatttgatt ttctttttcc acagatactg tgtaaaacta
    14761 cgcggatagg attggaaaaa taagggataa aacatatcca cagcctgtgt ataaagtgga
    14821 taaattctta taaaacgtat gtttgcctaa tgaatgtgat gaaataaggt aaaataacac
    14881 tgttttctgt ggataaacct gtggatgttg tgcataactg ttggggaaat ctttttaaca
    14941 aaaatagcca aaataaacat atagacgaaa aaattgatta taataaacaa agtgagcatg
    15001 aagaccaaag gagagaaaac atgaatatca aaattatctc agtaggaaaa ttaaaagaaa
    15061 aatacctgat ccaaggtatc aatgagtatg tcaaacggct gaatgcctat gcaaaaatcg
    15121 aattgatcga agttcctgac gaaaaagccc cggagaattt aagcgaagcg caaatgagac
    15181 aagtaaaaga aaaagaaggg gaacggatct tagcgaaaat caaagaacaa gaatacgtct
    15241 atgccttagc aattgaaggg aaaaatccta caagtgaatc ttttgctaaa caaatcgacc
    15301 aattaggtat ccaaggaaaa agccaccttg tttttgttat cggcggctct ttaggactca
    15361 gcgaagccgt catgaaaaga agcaatgctc agatctcttt tggaaaaatg acgtatccac
    15421 atcaattgat gcgtctgatc cttgtcgagc aaatctaccg tgccttccgg atcaatgcgg
    15481 gggctccata ccacaaataa tgatattttt taagagaaaa aattatccct attttaaata
    15541 atagcaatag gtagtggtag ggagaaattt ataaataact tttttatttg ctttggtagc
    15601 caagtaagtt gcaaaagaaa aatagatact gttaaaaaga taatgtagac atagaaacaa
    15661 atacgtaaaa ttttagttag aataatggca aggagaaaaa acatgataga tgaaaaaaag
    15721 tgtttaaaaa atctattaga ggatatggat gatattgaag aaaactatct aaatggactg
    15781 ggaaaacaaa tatatttcag atacttaagc tatctaaaaa tgaaataaga catagtaatt
    15841 ttttagcttt tttgtttgct ccaaatgaaa cccacaattt atctgatgag ttttttaaaa
    15901 tgtttttgaa aaggtatata gattctaatg atgataccaa agctgctatc aattattttg
    15961 atgctttact taatagctat gaagatttaa ttgtttacag agaaaataag aatattgata
    16021 ttttattggt atcggaaaaa aataaaatag tggtgtgcat cgaaaataaa attctgtctt
    16081 cggagtctgg agggcaactt aataaatacc aaatatatat agaacgcaat tattctaact
    16141 ataaaaaaat ttttgttttt ttaactccag atggaaatga acccacaa
//
LOCUS       DACDRK010000055        16142 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDRK010000055 DACDRK010000000
VERSION     DACDRK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.055"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            154..468
                     /locus_tag="IWJ77_001936"
     CDS             154..468
                     /locus_tag="IWJ77_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453530.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(902..1268)
                     /gene="ssrA"
                     /locus_tag="IWJ77_001937"
     tmRNA           complement(902..1268)
                     /gene="ssrA"
                     /locus_tag="IWJ77_001937"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1439..2212)
                     /locus_tag="IWJ77_001938"
     CDS             complement(1439..2212)
                     /locus_tag="IWJ77_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9453531.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2356..2706
                     /locus_tag="IWJ77_001939"
     CDS             2356..2706
                     /locus_tag="IWJ77_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9453532.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2687..3403
                     /locus_tag="IWJ77_001940"
     CDS             2687..3403
                     /locus_tag="IWJ77_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9453533.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3403..4851
                     /locus_tag="IWJ77_001941"
     CDS             3403..4851
                     /locus_tag="IWJ77_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9453534.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4921..5430)
                     /locus_tag="IWJ77_001942"
     CDS             complement(4921..5430)
                     /locus_tag="IWJ77_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453535.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5420..6145)
                     /locus_tag="IWJ77_001943"
     CDS             complement(5420..6145)
                     /locus_tag="IWJ77_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453536.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6368..8488)
                     /locus_tag="IWJ77_001944"
     CDS             complement(6368..8488)
                     /locus_tag="IWJ77_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9453537.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8718..9896
                     /locus_tag="IWJ77_001945"
     CDS             8718..9896
                     /locus_tag="IWJ77_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9453538.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9912..10670
                     /gene="aroD"
                     /locus_tag="IWJ77_001946"
     CDS             9912..10670
                     /gene="aroD"
                     /locus_tag="IWJ77_001946"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9453539.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10693..11178
                     /gene="ybaK"
                     /locus_tag="IWJ77_001947"
     CDS             10693..11178
                     /gene="ybaK"
                     /locus_tag="IWJ77_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9453540.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11249..12502)
                     /locus_tag="IWJ77_001948"
     CDS             complement(11249..12502)
                     /locus_tag="IWJ77_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9453541.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12620..13969
                     /gene="gdhA"
                     /locus_tag="IWJ77_001949"
     CDS             12620..13969
                     /gene="gdhA"
                     /locus_tag="IWJ77_001949"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9453542.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14081..15427
                     /locus_tag="IWJ77_001950"
     CDS             14081..15427
                     /locus_tag="IWJ77_001950"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9453543.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15735..16121)
                     /locus_tag="IWJ77_001951"
     CDS             complement(15735..16121)
                     /locus_tag="IWJ77_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9453544.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5272 a         2848 c         3232 g         4790 t
ORIGIN      
        1 cagttcaaac cagatacatt ggttaccatc tgtattaacg attatgcctc taaacgcttt
       61 ttttatgaaa aaatttataa gtgaaaacgt aggtatgata ttatctagag gtaatttttt
      121 tgggtattta aatagttgct aggaggatag cttttggatt tttacagtaa ttttatctta
      181 ataatagcaa tattattact tctaaatatt tggttttttg ataagagtag aaatgcaggg
      241 ataggtttta gaaccaaacg aagtacgtct tctgaaaaaa agtgggtata ttcacagact
      301 attttttacg gaggggttat ttcaatcagt ttactttctt caacacttta ttcttttaat
      361 gttatagacg tttccatgtc aaattttata tctattattg gaatccttat ttctgcaatt
      421 attacacaac tacttcttgt gtttgaagag aagtcaaaaa ataattaaag caattatgcc
      481 ttttagtaca ggaattatta cttcttacaa atgtatagcg aatgtgaagc ctgctgtttt
      541 aattgttcta gaaaaacttt tgagtgaaca attattttat gttaggatga attaatttct
      601 ctagagttta taattaaaaa acttatttga aggaacatgc ttaaaccata attgtgtttt
      661 tccttccatc agcataaaag ttcacctcaa aaagagtgta cgaacaaaag ttctttttgt
      721 aaaccttaaa atagattttc ctgaatacgt attggggatt tctaaatgtt tgaatgtttt
      781 tttgaatgca aaatcaatgc aaaaaatttt gtatttctct acttttctct ccttgaccaa
      841 aataaaaaaa cgttgattta acaacgtttt tcatacatgt ttatctgtct caaccataca
      901 tatatggaga cggcgggagt cgaacccgcg tccaaacaca tcgccactta aaaatctacg
      961 ttcatagtca actcatttaa ggattcactt tacagcttgc cgagtgacag gcattctgta
     1021 tcgctagtct gatgttctct ttttcaactt acagacggaa agttgaaacg tatcccacta
     1081 aagttaggac ccttactcga gcacatgggc gatgccgaga ggatctacgc taactgtttt
     1141 taggcagcta aagcgtaaga gttgttttcg tttttagcag ttatatttaa ctgtaacgtt
     1201 tttacgtaga cgtaacctac gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta
     1261 acgtccccgt tagctaacaa gggttacgtg tatttttcat acaaaattag tatagcatat
     1321 attgggataa attcaataaa aaagcgaatc atatcccatt atttatataa taactgcccc
     1381 tttcttaaga aaaaataagc gattttctta agaaaagagg tagcttcaaa aaaagatctt
     1441 aaatcagatc aaaatacttc aatgcgtgga caattccgcc ttctgtattt ttctttgtga
     1501 taaaggtaga aagttctttt aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc
     1561 aagcttccaa caaggcaaaa tcattcggac catcgccaaa tccaaaagta ggaacttttt
     1621 ccagctgaag ttctttttgc agacgtttga cacctgcccc ttttgagatt cctttcttca
     1681 ctgtatcgat cgagtaagga ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat
     1741 agtattcgtc acctgattga gataagatca gcaacatatt tactgtgttt tctttaaagc
     1801 ctaaaggatc aatttccgga acatctgaat gaatgaagtc gtaagcattg atcattgttt
     1861 ggctatgacc ggtacaccag attttctgat cgttatagta agaaagttca tgaccgttca
     1921 actgtacatg atcatacatt ttttgacatt cttctgaaga aaatgtttga gaatagattt
     1981 cttcgccgtc tactcgaatg aaagaaccat tcatggtgac cgcagaagta atgcccgcat
     2041 cttccatgat agagtggatt tctgcttctg tacgtcctgt agcgatgacg gggagaacat
     2101 tgttttcttt taatgcgttc atagcttttg ccacttcggg tgtgattttt gatttcgcat
     2161 ctagtaatgt tccatcaaga tcaaagaaag tgattgcacg ataatttgac acaaataaaa
     2221 ctcctttttt attggaataa gtttatctta acaaagaatg aacaaaagac aaacctttcg
     2281 tgaagaattc ctgaacatat tggttcagtc aacaatttag agtatactat aagaagagaa
     2341 tttaggaggg aaagaatgat ttttttcagt attggtatct tattcttaat aattggcagt
     2401 ctttttcgga ttctgccctc aaaaggaaat ctgccttttt atgggtatca ttcgcctctt
     2461 gccgcaaaaa cagacgctca ttggcggctg gctcaaaaga caagcggaaa ctggtttttt
     2521 cttatgggct tactgatggc attgatcggc tattatttaa agacatctgg gcatacgaat
     2581 tattttctga ttgaaatgct tctgctagta tttccaatta tgcctatttt tattatgaca
     2641 gaaaagaaat tacagaaata tgacctggaa actggaggga acgataatga atattttaat
     2701 gattgaagat aatcaatctg tttctgaaat gatgcagatg tttttcttaa atgaaggttg
     2761 ggaagcaact ttcaagtatg atggaaaaga agggctcgat gcctttttag aaaacccaca
     2821 acattgggat atgattacac tagacctgaa tttgcccaca ctggacggta tgctagtagg
     2881 aagagagatc cgcaaagttt caagtactgt gccgattatc atgctgacag ctagagattc
     2941 tgaaagcgat caggtgatcg gcttagagat gggggcagat gattatgtca caaaaccttt
     3001 cagtcctctg acgcttattg ctcgaatgaa agctttgcat cgccgtagtg agttggcaga
     3061 agcaagagaa gaagcagcag tttcagatga tcattttgat atcgagacta gtcatttcaa
     3121 gatgaatacg aaaacacgag aagcgtatct ggacaaccgg ccaattgaag gactgacgcc
     3181 taaagagttc gatctattgt ttactttagc aaaaaaacca cgtcaagtat tctctcggga
     3241 gcagttgctt gaattagtct gggattatca gtactttggt gatgaacgga cagtggacgc
     3301 ccatatcaag aaattacgac aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt
     3361 ttggggagtc ggctacaaat tcgatgattc tggtgttgcc taatgcgtta tttgtatcag
     3421 caactcttag ctttttgcgg aatggtcgcg ttgatcttat tgatcgttgg agtttctttt
     3481 acacaactga caaaacaaac gattgaagaa aacaattacc agcaactgtt tggatatgct
     3541 gaatcagttg aaaagacaac gcaaacctat gccgatacgc tcccgcagtt tagtcaagat
     3601 caggcgtttc aaaatgcatt gctgctgaca gagcaagtac tgacagaaca aaatgtgaac
     3661 tttatattta tcgataaaaa cgaacgtgtc atttatccga ctaatacagc tttactgaat
     3721 tttaccttaa cagctgatca atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca
     3781 tcgaataaaa atattttagg acaagcacag gcgacctctt acgcacttgt gccatttaat
     3841 ttgaatcatg aattctatgg agcacttgta gttagtcaac ctgcgcgaaa tatcgataac
     3901 agcgttcgtc caattacact gaacctgttc aaaggattta ttttttccag tatcgttgca
     3961 gttattgcca gctatgcttt tgcagcattc caagttaagc gtatcaatcg gttaaggaat
     4021 gcgacgaaag aagtgacgaa tggtaatttt gatgtacagc tccccgtaca tgacaaagat
     4081 gaatttgatg agttagcaga tgatttcaat aaaatgacga attcattgaa agaatctcaa
     4141 gcagaaattg aagaacaaga aaatcgtcga agacaattta tggcagatgc ttctcacgag
     4201 atgagaacac ctcttacgac aatcaacgga cttctggaag ggttagaata caatgcaatc
     4261 cctgaaaatc agcgagaaaa cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc
     4321 cgtttggtga atgaaaactt ggattatgaa aaaatccgta cgaatcaaat cagtatggtc
     4381 attaaaaaat tcaatggaac agaaacattg aaaaatattg ttgcacagtt agaagctaaa
     4441 gcagaagcag caggagatac attgacattg aaagccgttg atgatattga tgtttacgct
     4501 gattatgacc gatttgtcca aatcatggtc aatattattc aaaacgcgat acagtttact
     4561 gaaaacggcc aaatcacgat tactttagaa aaaggctatc ttgaaacgat catcacgatt
     4621 gaagatacgg gaattggaat gtctgaacaa caaatgaaaa gtatctggga tcgttattat
     4681 aaagtagatc cttcaagaaa aaatacaaaa tatggtgaat caggattagg tttaccaatc
     4741 gtgcagcaac ttgtacgctt gcataaagga aaattagaag tagaaagcga actaggaaaa
     4801 ggaacaaaat tcgctgttcg tttgcctgac gtagaaatca aaaaagagta aaaaacaagc
     4861 ggtataaaag atcatgaatc gatcttctat accgcttgtt tttactataa aatgattgat
     4921 ttagcccgcg ggctctaaac gatcaatata aggactttga gcaatagtag catcatgagt
     4981 aaagagtaag ataccttgat tttcttcatg agctaaataa gaaagcaagt ggagtagctc
     5041 ttggatctcc aatacactaa gttcatcaaa tgtatcttca ataacaatat tgtttcgctt
     5101 ggaaagcaag gcatgaagta attgcagctt tactttttca aaagacgtca actcagctgc
     5161 cgcacaatta agcaaggaag gagagatatc aagtttttct tgataggcca aacgatcttg
     5221 tttgatttct ttttccggta catccaacag gacatggtct ttaagattca gataaggaaa
     5281 catcggccaa tttttttgga tgatcgcaaa attatccagt tttgcaacaa gaacctcttt
     5341 tttcaagtgc tccaatattt tatgctgttt ttcgagagga cacaaaataa cggtcaagtg
     5401 ttcagcatga atcaaatatt caggtttcat tacgtccagg cctcctactt tgcaaatgat
     5461 ggataaaaaa agtgaaaggg aatagactaa gaatgagaca gcatccactg agcccaataa
     5521 gagtttgggc aaatccctta agtatatctg tgaaaaaaag atcattttgg aaatcgatgg
     5581 taaaaaaagt ctgttgatcc atcggaattg taagtccgct taccgaagtc aagatatcac
     5641 ggatatctgg cagttcagta gtctttaaga caagaaaatt gatcctttga agaagcgctt
     5701 cataaactga ctggaagctt acgatccaca ataaaaccaa gatacaacaa agaaagagag
     5761 gaaagaacag ctgccagaaa ataaaaagga ataatttggt tttagaaaga ccagtcaggc
     5821 gccaattgat caattcctgc ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa
     5881 ggaaggcaaa tagaaaaaat aaactgaata tgccttttat ataaaatcgg ctagctaatt
     5941 ctaaagccgg aagaagctct gtagatgcag aagcgccaat aaatgaaagc cgatcttgga
     6001 cttgattggc taaagccgat tgcaaatggt aaagagtcag gcatgctatc aataaaaaag
     6061 cagaaaagct tgaaaataaa caatagagta ttgtctgctt tttgtaataa attaatcgag
     6121 cagtagcata gcggattatt ttcatacttt tcacctctat aaaaagtata gaaaatgaat
     6181 atgaataaag tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa aatattttgt
     6241 aaaaagcaag aggttgtgaa gaagctgccc tattctatta ccgtagatgc aagcttttga
     6301 acactgttta ttcgaaaaga ggagcagcga caataatttt gtcgctgctc cttttaaatt
     6361 tattttgtca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac gtttgcgttt
     6421 tataaagatc aacagttgat tgtccgttgt gttcactaaa gacactagtt gacttgtcac
     6481 caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca taatcactgg
     6541 tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca ttattgattt
     6601 ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt tcattctgta
     6661 cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc gtaccaccgt
     6721 agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc tgttcatgat
     6781 ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca cctagtaagt
     6841 gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt ccgccctgtg
     6901 tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg tcgtagtttg
     6961 tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt gaattcgaga
     7021 caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg ctttctttca
     7081 aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca aagtaaccgt
     7141 tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg gtgaattttg
     7201 aataaggata atggttagat acagcaatga attttgaata aaatggttgt tgcagacgtt
     7261 ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat tggaacgaat
     7321 tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct aaattcttgt
     7381 atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca ctagtgtaac
     7441 cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg cctaattgtg
     7501 tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt tccgcatcgc
     7561 ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg ctgttgctgt
     7621 ggtaaagact attgatgaaa ggtgtaactt catgctggag accattctca tctgtcaatt
     7681 tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca ttttttcctt
     7741 tagcgagccc gaacaaatcg tcatttggtg ctgcataatg ttcttttaca taattttcta
     7801 cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg taagtttgaa
     7861 caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa tcacgagaga
     7921 atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt cctgagaaaa
     7981 tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg cgagggtcca
     8041 acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc caatagatca
     8101 catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct actttcccag
     8161 cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa gtgacattcg
     8221 aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa gcaactctag
     8281 ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata gggttgataa
     8341 acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact aaataggcaa
     8401 aaatcgtttt caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc ctcttattca
     8461 aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt cttaatcaca
     8521 gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta ctagtatgac
     8581 aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt ctttaagctt
     8641 catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg gaagagaaag
     8701 ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat cgattgaaaa
     8761 aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa accacgcaat
     8821 gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta ggaaagcaga
     8881 ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa tctttttttg
     8941 aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat gattctacta
     9001 ccgcttttcg tatattcaat ggagaaggag acggaatcgg cggtctgatt gttgattatt
     9061 atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat cgtgaattat
     9121 tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag aaaattcgat
     9181 ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca ccggaaccct
     9241 tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga ttaatgactg
     9301 ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc gcttctggga
     9361 aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc gcaatgggag
     9421 gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa acacaggaac
     9481 agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg gacgtattcg
     9541 agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta ttggatccac
     9601 caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat ggggaactgg
     9661 tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct acaaatgctg
     9721 cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat gccaaaaatg
     9781 tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg acggatacct
     9841 ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac cattagaagg
     9901 agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga ccaaagattt
     9961 gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt cttgcacgtg
    10021 aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt ttttcttttg
    10081 atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg ccgttattat
    10141 ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt cagtatcgcg
    10201 acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc gagttgttca
    10261 aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac attcctttaa
    10321 ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt taccgattga
    10381 atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc aataatgaac
    10441 gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc tccatgccta
    10501 ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc ggaacgacgg
    10561 gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca ggacaattac
    10621 cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag aacaagtttg
    10681 aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg tatggtcgaa
    10741 caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca cctaagtgcg
    10801 tcatcggtcg ctgaacagct tccagaaagc caatctcgta tatttaaaac actggttgct
    10861 gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga attgaatcta
    10921 aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt aaaagatttg
    10981 gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa aaaactattt
    11041 cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc ggctggcaga
    11101 agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag cggacaattt
    11161 gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg atgttgtttg
    11221 tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc attcctgcta
    11281 catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca ttgacatcca
    11341 gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt ttaggataaa
    11401 tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga aaagttttgt
    11461 tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt ttttgagagg
    11521 cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact aacaatcgct
    11581 caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta cttttttctg
    11641 tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga gaaagtgtca
    11701 ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc agtgcagccg
    11761 gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc aatacacgac
    11821 cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga agtgttttct
    11881 cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga ggcgtcaccg
    11941 tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta gatggataag
    12001 tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat tccgtgcgta
    12061 taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag aatagaagcg
    12121 tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt gatttatttg
    12181 ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt gattcaaaga
    12241 agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac tttccatttc
    12301 caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg caccgaccac
    12361 cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag agtacgcgtg
    12421 cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg ccgactacga
    12481 tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata tctgaagaaa
    12541 gaaaacagaa aaaaaatgtt ttgagccaaa agagaaaatg agctaaaatg agggaagata
    12601 agcaaaggag cgtggaacga tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa
    12661 gctacatcaa aaggatcaag ggcaagaaga atttttacaa gcaatcgatg aattcatgcc
    12721 cacggttatg tcttttttgg acactcatcc cgaatatatc gaaaagaata tcttagaact
    12781 tttaacggaa ccagaaagag ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa
    12841 ctggcgagta aatagagggt accgtatcca atacaactca gctatcggcc cttataaagg
    12901 tggattgcgc ttccatccta gtgtcaatct aagtatcttg aaatttttgg cttttgaaca
    12961 gattttcaag aatagtttaa ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt
    13021 cgatcctaaa ggaaaatctg ataatgagat catgcgtttt tgtcaaagtt tcatgctgga
    13081 attgtcaaaa catatcggcc catcgattga tgttcctgcg ggagatatcg gtgtaggagc
    13141 tagagaaatc ggctatttgt tcggcgaata taaacgattg aaaaaatatg atactggtgt
    13201 gttgacaggt aaacctttgg atttttgggg aagtaagata cgtacggaag ctactggtta
    13261 tggattagtg tattatgtca aacatctatt gaacgaagaa aaagattcat ttaatcaaaa
    13321 aacagttttt gtttccggaa gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga
    13381 gctaggcggg aaagttgtta catgttcaga ttctagcggg tacatctatg atccagaagg
    13441 aatagatgtg aaactattga aagaagtcaa agaaatcaat agacaacgtt tgacggaata
    13501 cgcaaaccaa cgtccttctg ctatttatca tgctggagaa tctgtttgga cattgaaaga
    13561 aaaagcggat atcgctttgc catgtgctac tcaaaatgaa atagacgaaa gcttggccga
    13621 aatcttagta gaaaatggga tcaaaatcgt agcagaaggc gctaatatgc catgtacagt
    13681 aaaagctgtt tctgttcttc atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa
    13741 tgctggagga gtagcagtat ctgctttaga aatgagtcag aacgtccagc gattgacttg
    13801 ggaaagagag caagtggatg agcagctgga tcaaatcatg gaaacgattt accatacgtg
    13861 tcgccaaaca gcagccgagt acggcgacga aaaaaatctg cttcttggag caaatgttgc
    13921 tggatttgaa aaagttgcca aagcaatggc agcagaaggt ttagtttgaa aaatgaaact
    13981 ttcttggaaa tattgtcagc attttgttta tttgcttgaa aaataataaa aaaaaacgta
    14041 aagtattacc tgtagagtaa acctataagg aggagaacaa atggcacaca ttcattttga
    14101 ctattcaaaa gtagcaccgt ttgtcaacga acaagagtta gggtacatgc aaagccaagt
    14161 aacagcagcc cacaacgaat tacgggaagg tactggcgca ggaagcgact tccgcggctg
    14221 gatcgattta ccaacgaact atgacaaaga agaatttgca cgtatcaaag aagcagctaa
    14281 aaaaatccaa tctgattctg aagtattagt tgttatcggt atcggtggtt cttatttagg
    14341 cgctcgtgca gcgatcgact tcttgaatca cacattcttc aatttattag atagcaacac
    14401 aagaaaagca ccacaaatct tctttgccgg aaattcaatc agctcaacat atattgctga
    14461 cttgatcgaa gtaatcggtg atcgcgactt ttcagtaaac gttatttcaa aatctggaac
    14521 aactactgaa ccagcgattg cgttccgtgt attcaaagag cttcttgtta agaaatatgg
    14581 acaagaagaa gcaaacaaac gtatctacgc tacaacagat aaagcaaaag gtgcagttaa
    14641 agtagaagca gatgcagaag gttgggaaac tttcgtgatt cctgatgaca tcggcggacg
    14701 tttctctgta ttaactgctg ttggtttgtt accaatcgct gttagtggtg cagatatcga
    14761 tgcattgatg caaggtgccg ctgatgcaag caaagcatac tcaagcgaca aactagaaga
    14821 aaacgaagct taccaatacg cagcaatgcg caacattctt taccgtaaag gcaaagtaac
    14881 tgagttgttg atcaactatg aaccaggaat gcaatacttc tcagaatggt ggaaacaatt
    14941 gtatggtgaa tctgaaggaa aagaccaaaa aggcatctat ccttcaagcg caaacttctc
    15001 aactgatttg cactctttag gacaattcat ccaagaagga cgtcgcaata tttttgaaac
    15061 agttgtgaag gttgaaaaac ctcgtaaatc aatcacaatt cctgaacaag cagaagattt
    15121 agacggtcta ggctacttgc aaggaaaaga agtggacttc gtaaatacaa aagcatttga
    15181 aggaacattg cttgctcata cagacggtga cgtgcctaac ttgctagtca aaattccaga
    15241 aatggatgct tatacattgg gttatacaat gtacttcttt gaaattgctg taggtatttc
    15301 aggttacttg aatggaatca acccattcga ccaaccaggt gtagaagctt acaagaaaaa
    15361 catgttcgca cttctaggca aaccaggctt tgaagatctt gctaaagaat tgaacgaacg
    15421 cctgtaagat aagggttttt cgctagattt aaataatcag tgatagaaac atgttcgctt
    15481 taaaatgaaa actggtaccc atttaggtac tcaaacatac tgcatcgttt attcagaata
    15541 aggggctgtg acaagacgtt tgtcacagtc ccttgtttgt atagaaatcg attgattgcc
    15601 ttgctcttta gtttgtttta ggactttaag aacagtgagg ctgacttcga aggttaattc
    15661 tgtttttttg ttttctagaa tcagtaagca aaaaagcact cacttcgagt gcttttttgc
    15721 gatatattct tagcttaaga taggcctaat ttttctttta cagatgaggg taccttatcg
    15781 ctggtaatta cttttttctc tatgatccgc ttttcgcttg ctttcaactg caagatcgtt
    15841 ccttcggggt aagcagaacc attggcagag tcaccagaag taatgacatc gaaatctaac
    15901 tgtctttttt caccgttttc agcataagca tctacgttgt atttaaaacc tcttccaatt
    15961 acttccccat tatctgcttt gatatcagtt tctgcaggca ttggagcttt gactaccgca
    16021 taataatctt tacctacata agtagactga tagtagtccc aacctttcca tcctataaaa
    16081 gcaatcaata tgataacgat caaccctaaa atttttttca tgaaagatct ccttttttat
    16141 tt
//
LOCUS       DACDRK010000056        15756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDRK010000056 DACDRK010000000
VERSION     DACDRK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.056"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            329..715
                     /locus_tag="IWJ77_001952"
     CDS             329..715
                     /locus_tag="IWJ77_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9453545.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            809..1000
                     /locus_tag="IWJ77_001953"
     CDS             809..1000
                     /locus_tag="IWJ77_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9453546.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1514..1846
                     /locus_tag="IWJ77_001954"
     CDS             1514..1846
                     /locus_tag="IWJ77_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9453547.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2142..2297
                     /locus_tag="IWJ77_001955"
     CDS             2142..2297
                     /locus_tag="IWJ77_001955"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9453548.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2573..3913
                     /locus_tag="IWJ77_001956"
                     /pseudo
     CDS             2573..3913
                     /locus_tag="IWJ77_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4154..5770)
                     /locus_tag="IWJ77_001957"
     CDS             complement(4154..5770)
                     /locus_tag="IWJ77_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9453549.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            5888..6106
                     /locus_tag="IWJ77_001958"
     CDS             5888..6106
                     /locus_tag="IWJ77_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9453550.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6301..6762
                     /locus_tag="IWJ77_001959"
     CDS             6301..6762
                     /locus_tag="IWJ77_001959"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9453551.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(6955..7194)
                     /locus_tag="IWJ77_001960"
     CDS             complement(6955..7194)
                     /locus_tag="IWJ77_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453552.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7218..8741)
                     /locus_tag="IWJ77_001961"
     CDS             complement(7218..8741)
                     /locus_tag="IWJ77_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9453553.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(8759..8881)
                     /locus_tag="IWJ77_001962"
     CDS             complement(8759..8881)
                     /locus_tag="IWJ77_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9453554.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(8911..9894)
                     /locus_tag="IWJ77_001963"
     CDS             complement(8911..9894)
                     /locus_tag="IWJ77_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9453555.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(9918..10067)
                     /gene="rpmG"
                     /locus_tag="IWJ77_001964"
     CDS             complement(9918..10067)
                     /gene="rpmG"
                     /locus_tag="IWJ77_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9453556.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10088..10465)
                     /locus_tag="IWJ77_001965"
     CDS             complement(10088..10465)
                     /locus_tag="IWJ77_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453557.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10497..10676)
                     /gene="rpmF"
                     /locus_tag="IWJ77_001966"
     CDS             complement(10497..10676)
                     /gene="rpmF"
                     /locus_tag="IWJ77_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9453558.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10691..10960)
                     /gene="rpsN"
                     /locus_tag="IWJ77_001967"
     CDS             complement(10691..10960)
                     /gene="rpsN"
                     /locus_tag="IWJ77_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9453559.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11483..13255)
                     /locus_tag="IWJ77_001968"
     CDS             complement(11483..13255)
                     /locus_tag="IWJ77_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453560.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13209..14963)
                     /locus_tag="IWJ77_001969"
     CDS             complement(13209..14963)
                     /locus_tag="IWJ77_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453561.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15073..15642)
                     /locus_tag="IWJ77_001970"
     CDS             complement(15073..15642)
                     /locus_tag="IWJ77_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9453562.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         5101 a         3008 c         2392 g         5255 t
ORIGIN      
        1 agaaataaaa aatacaatct atacacgaat aagaatatta tccaaaagga ggttttagaa
       61 aagatttatt atacgtaaaa agctcaagac attttcttag ccataaggac atgtttgata
      121 tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca ttatcaacta
      181 aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag cttatagaag
      241 ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc tcttttccct
      301 ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg taaatgccca
      361 gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt ataaaaaaca
      421 agaagaaatt caaaaacatg aacaacttga tcaactccca ccatatttgt ttactaaaga
      481 tactataaaa tttaaacaac cgattaatgt tttaaatgtg actctccttg ttgaagatat
      541 aaaattagca aaaatccttc ctttgttgaa agaacctgaa caaatttttc ttatcgaaaa
      601 atttatcttt gataaaactg ataaagaaat tggaaaggtt ctggggatta caagacaggg
      661 tgcgacaaat ttaaaacatc gactttacaa aaaaattcaa aaaatgctgg tttaatatct
      721 gcataataac taattttgta tgcttctttg ctcgttctcc tccttcccaa agaaatagat
      781 tataagtcca tgggaagaag gtagatttat gaattcttta aaagaagatt tcattttagc
      841 aaaggctgga aatgaagaag ctgttgaggc aattctcaaa agatttagct ctttgattca
      901 taagcaatcc tggagaactg gaaaatatga tcaagattgt tatcaagaat gtatgcttgc
      961 aatatatcta gctatctcga aattcgaaat taaagaataa ctcaactaag ggagtctgtg
     1021 acataagtaa aaagatagtt tcaaatttcg aacaataaat ttgaaactat cttctttata
     1081 attataaagt atcgatttta cagcgttcta taatgattca tagaatctaa aacgaatgat
     1141 tttaattatc aacgtttttt aattcgtttt tagcttaaaa ataacttttg tcacagtctc
     1201 actcctaaaa ttactttatt tacgtatgaa gtgggcagga ggtcgaatcc agaactgggc
     1261 tatggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt taccttccgt
     1321 aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag
     1381 tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt tagttgacaa
     1441 accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac gttaccggaa
     1501 ggaatgaact ctattgagaa aaacatatga tcgtaaattt aaactaaaga tttcacagga
     1561 tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata tctctcgtcc
     1621 aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt ttgctggaca
     1681 aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa ataaacgact
     1741 aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc agggatcact
     1801 caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt
     1861 gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg tagagaaaaa
     1921 acaatcaaag aaatggtaaa aaaatgttga atcgataatt tgaacagaac caaattgatg
     1981 ctgtttgggt gatggatatt acttacattc catgtaatga cggtcgcctc tatttgtcca
     2041 cctatattga cttagcaact cgtattctac gttactatat ggtagattca cacatgaaaa
     2101 aagagattgt cgtccgtcca ttacaaattt attaaggaga attgcctacg gtaattcatt
     2161 ccgatagagg tagccagcat cggtctttta tttatcaaga gctgttgaac gaccaccaca
     2221 tcacacattc agtgagccaa actgggactc ctgttgacaa tgcagtaatt gaagcgtttc
     2281 atcgatcaat caaatgagaa ttaatcgact ccaacaaata taaaagtaaa atagagatga
     2341 aggtgttaat ccaagattat ttagaagact actacccaaa caaaagaatc catacaaaat
     2401 ttatgatgac tccttgacaa tttgaagcga agaaaataag ggatctataa tatctagtgt
     2461 tggtgacgcc ttctgttgcc aatacttttt cttttttcta ataaacgcaa acagacacct
     2521 taaaatcctt tagaataaag tcgacgaaaa ccattcaaag gagtgtttca agatgtccta
     2581 ttcagagttt accaaagagt tactagatat tctagaccta aatcttatct ttcatgaaga
     2641 tgcctttcgc aaagagcgaa tcaatgacga aacatgtttt gtttttgatg gcacattgac
     2701 ctatcaaccg gaggaatgct ttcatcgcca ctatcaaaat gaacaaacga tcattaaata
     2761 gggctggaaa aaagtatcca tcttattgaa tgatgtcagt aactacaaga ccattctgcg
     2821 gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag acttttttag cagaagattc
     2881 agttagtgat cgtcgctgct ccattgctcg cagagtcaaa caagccattc ttgagctatt
     2941 aagcgaacct ctttctatgt ctttgatcgc acgtatgaag cacatttctc cgaccactgt
     3001 cattcgaata cttcgcagcc ttcggcctaa aaccatttct ttgagccagc tgttaccaga
     3061 agtggtgtgt ttcgatgaat tcaaatcagt gaaaaatgtc tcgggagcca tgagctttgt
     3121 catgatggac ggcaagaccc atcaactgat tgatattgtg gaaaatcggc aactcaatcc
     3181 tttacgcgac tatttcttgc gctaccctat aaaagtccgt gaacaagtgc gtctagtggt
     3241 ttcagatttc tatacaccct atcgcacatt ggtgaaagag tgttttccga atgcccgaat
     3301 cgtcgccgat cgctttcata ttagccaaca cattggtcgg gcttttacca accaccgaat
     3361 tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg aagcacttaa agaaatactg
     3421 gaaactactt caaaagaatg cgtgggaatt gaaaggtcag catcgctact ggcgtccgtc
     3481 atttcgagat catctgacag aagcagagat agtcgatcgg ctattatctt atgatgattc
     3541 cttaaagcga ggctacgaag tctaccagga ttttctttcc gttatcagaa gacaagatgt
     3601 tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag ttgccagagc actaccaacc
     3661 agtgttcacg accttcaaga aataccaaac agaaatcaaa cgagcgttgc gcgttcccta
     3721 ttccaacggc cctatagaat gtttgaataa ccatatcaaa gtactaaaac gaattgccta
     3781 tggctataga aactttcaaa actaccgaga acgaatcttt ttatatcgag gaaaatattt
     3841 taagaaaact aagaatacta cccaactgac taaagccaga acaacaacac ggctagacaa
     3901 gatagctgtc tagccgtgtg agtatctcga ttattctatt gaatttcttc accaacactc
     3961 attgacgaag agccaaaata agtaatctaa ccgactgtta actaaaccat tgcaggtccc
     4021 gttaaagata cttgtgcctg ttgtcaccgc ttctttttct tgtccacaag cgcttaacac
     4081 acttaataaa gcccctgcta ttattaataa taatatcttt ttcttattaa aaacctcctc
     4141 atctatttcc agattagtaa atgtagtaat ctgtgctttc ttttttctca atactaataa
     4201 ctaacttatg gggtaaacaa atactcgttt gaccgggttt tgagatccat ccagttctaa
     4261 ccgcgatttg atcaggacta ttatcttctt taactcgaat tcttccatct ttaatctcaa
     4321 caatattata ttgtcccttc gcaggatgat aattcacgag tttatgattg atcttatcca
     4381 aatcgaagcg atccatttcc ttgccatcaa ttcgaacgat ggcatatttg atagcatctt
     4441 cagttgtttc attctccttt tctatagaga atacaaataa gggaataaaa gaggtaataa
     4501 tcaaaatgcc aataattatg acatcgccta tcttgacacc agcttttctt aaaaatttca
     4561 ttaacgatat ctggctaaac atattagact ttttccatgg aaatggtgaa atttttcgga
     4621 aattatctgc attaagagaa tcagttaaac aaccctcaag ttgttcctta atacattgtt
     4681 gatccatctt cataccaata aaaactagtt tattaatgcg ttcaccaaac aacggatgcc
     4741 aggcttcctt caattcggga tttttttgta taatcatttg acactcaatt ttaggtaaac
     4801 tagctaccca ataatttaat gattcaatac ttttatagct acccgatgtt tcaaagatcg
     4861 tcgaaaaatc actatgttgg gcaaaccaaa gaaacccttt tgctcttgta atattgccag
     4921 gaaaattttt atccgtaaga tgggcaaatt tttgtgcgtc aaggagtaac cgatttcgat
     4981 agataaaact tgagatacca tattcttctg tttcaggaat atgattttga tagccaagct
     5041 ctaactcttt gaaccagccc actgaatagg aagtctcatc tatattaaaa agatttgtat
     5101 ttagaatttt cgataagtct acttcacctt taattgtcct aattattttg gcattggact
     5161 gtaacttagc aattagaccg ttaattttgt aaatcgtctt ggttgacact aagtcactat
     5221 tatttaaaag taatatgtta caacactcga attgatcaat taacaagcca cgaatatcct
     5281 gttcttcttc aactacatta ccttcagctt ttctttcctg aatcttttca cccgtttcga
     5341 aattatccca aaaacgtttt acatccacta ctataataat ggcaccaatc tctaatcgtt
     5401 gggtcaaatc aatgtctaaa gggtttttag ctaatataat tgcttgaata attggaattg
     5461 gtttgctgat tcctgaagct tcaataacaa tttgattcaa gtcagaaaat tgcactaatt
     5521 gatctagttc aacaatcaaa tcttcgcgta atgtacaaca aatgcatcca ttcggcatcg
     5581 aaacaagttt ttctccagtt cttttaaaac ctttcttgtc gactaatacc ccatcaacat
     5641 ttacttcccc tatgtcattt acaatgacag caatttcctt tccgtgatca ccgtacagaa
     5701 tatgatttag aagtatcgtt tttcctgaac caagatagcc agttaagatt gtaataggta
     5761 tttttttcat agggaacctc tttctatacc ttgaatttac actttaagtg caacacataa
     5821 aaatgagtca taatcgatat ccaactagta taagtttgtc gagaacactc ctagaaggaa
     5881 aataattatg agccagtatc gtcatcttac tatactcgaa cgagaaagaa tctttctgct
     5941 tcacgaagaa ggattttcta ttagtataat tggcaacgac attcagcata gcccgtttac
     6001 tattttacat gaactgagaa gaaacaagaa aaaaactatt caccttccaa agcggaaaaa
     6061 aattatgctt atcgaagaaa aaaatgcgga cgttatcaca ttctaaatga tccgtgagtt
     6121 tggtcaattg ttcgtcattt atttgtcgat tttcagtggt cgccagagga aatctcgaat
     6181 cgtttgaagc atgaacatat ggacttttca attagtttta tcacgatcta acgtgcaatt
     6241 tgcaatgatc ttttcgatac agaacccctc tcacacggca ataaaggcct gatccgttca
     6301 ttgcaccatc gaggaaaaac aagacataca aaaaatcatg tcgagtctcg tggaaagatt
     6361 tgccttactc attcgatcca tggccgtcca aaatccgcag atgatagaag tgattttggt
     6421 cattggaaag ccgatattgt agctggcaaa actggtggta cttgtttagt gactttgaca
     6481 gacagaaaat catgcttcct tttagcagga aagaaggtcc ccagaaagaa agccgcttat
     6541 gtgagcgaga aaatgattga acttttaagc agtattccaa atgaggcggt caaaacgatc
     6601 actccagata gaggaaaaga attctccaat catttcgcag ttacagaagc attgaataat
     6661 atgccctttt attttccaga tcctcatgcc cttggcaaca atgaacaaat taaaatacga
     6721 atagactcat tcgagaatac ttgccaaaat cgaaagagat agctacggta gataactcaa
     6781 cgataaatca gatgattgaa aaactgaata acagacctag aaagtgttta gattggagaa
     6841 caccttatga agtcttcttt gataaagtgt tgcgcttgat ttgacaattc aagacataat
     6901 aaaaagcaga tcaaatccaa ataagggaaa aatgatctac tctggtatta tcaattaatt
     6961 ggccaagcta ttatgccgtt ttgctaaacg tctacttctt ccagtattga ccttgataaa
     7021 acaacaatca ggatcccgtt ttaatactct atgaactgtg tcaatactaa catgtaaagt
     7081 tgcagcaata tcagctaatg tactcccatt taatctcatt tgctttgcaa atcgataacg
     7141 ccgataagca tcaatattct tggaatccat agacaataat acttttctta ccatgtattt
     7201 cctccaaaaa ttgaatttta atgagccatc atttcttgag caatttcatg tttagacaat
     7261 gaagctggat agaaagtagg ccaattatgc aattcattat agagtttttc ttgactttct
     7321 ccaccaaaga aaatatggaa atgctctgcc ttacttggtg caattccatg atcactaaat
     7381 tgaatatatt taaaacgacc agcacctgca tcatcagttt caaaattaaa acgaacacca
     7441 cggtttcctt tttcataatt caaaattttg tatccaacat atttgtatgt gtattgatga
     7501 tgttcaccgt tgactacaaa atcaatggtg ttgtctttaa tgttgatagt atcaatatct
     7561 gtcttatagc cagtagtata ataatctttg tactcggcag ctgtcatgtc tttattgatt
     7621 tttgctttgt aatcgaaaac ttcatccaaa attccatctt ttaacaacgg ataaacagat
     7681 tgccattcgc ctgtataatc actcaattcg cgatccttca catcggcatc tttaaagtac
     7741 ccattagcaa ctgtcttttc atccttcgct tctttttctg gaactacctc ttcaccagca
     7801 accatagttg ttttttctaa cgcttttaga ttgtcttcca ttactgaaac ataatcttca
     7861 ccattatcca tttgttcttt tgtaagactt tcaagaggat tcagtacttc caattttatc
     7921 ccagcttcgt tggccaaagt ttttgcaatt ttatcattag cattcttttc aaagtaaata
     7981 taattgatct tatttttttt aacatactct tttaattcag ctaatcgacc agaagatggc
     8041 tcttcttcag gacttagtcc agcaatagga acttgtttca atccataatc caaagctaaa
     8101 tatccaaagg ctgcgtgttg cgtcacaaaa cttttttgtt tcgcttcttt aagactagtg
     8161 gtatagtcag catccaaccg tttcagttta gtcaaatact tttcagcatt tgtctcaaat
     8221 acttttgctt ttttcggata caatttaact aactgatcct tgatatttga aacttcctta
     8281 attgccattt tgggtgaaac ccacgtatga ggatctagtt catgatggtg atcttcttct
     8341 ccgtgatcat gatcttcttc ttcactacct ggcaataaaa tcatgtcttt tgttccttct
     8401 actacattag gcccagcttt cttccaactt tctttagctt ttggtaccca tgattccatg
     8461 ttttcgttat gatatacaaa aacatcagca tcatgagctt ttgccatatc cttagctgag
     8521 ggctcatagt catgaggttc tgatcccgct ggaaccatta aatccacgtt cgcttcatcg
     8581 ccgacgatgt ttttggtgaa atcatacatt ggatagaatg ttgttacaat ttctaatttt
     8641 ccatctttat cattcgatac ttttgcatca ttagtgctgg agcaagctcc caataatcct
     8701 agagctgcta tcccaataag tcctacactg attttcttca ttctttttcc ttccttaatt
     8761 atgctaattt cttcatttta tttcgtttat gttctttgat aatttttaat gccctttttc
     8821 tagttttaat attcgggctt ttaagtcgtc gatatgcact agatatatcc gttttctcca
     8881 ttagaaatta cccccaatca ctacttatca ttatttgctt tccgccagta atttatcaaa
     8941 actctgtttg atcctttctt catccaacat ttttccgatt agaataatag tggtttggtc
     9001 tttttgtcga gtggtgtatt gaaaattaac ctgttcgttt acctcttgca tggcaactat
     9061 aaaatcctgc tcttgtaaag caatcaatcc tttaaaacga tacaggctcc cttggttggt
     9121 taaaatcagc cagtcaatcc attggagtaa aaaatctttt tttatgtctt gatctgaagt
     9181 taagagaatt gatttaaact catgtacttg atggtgttga tgatagtatt tgccattata
     9241 gtgattttct gatgaatcac tttcaaaaac taagggatga ttaaattttt tcaaattata
     9301 aaaatccttt ttttgaatcg tttcactatg tgtgaacatt cgaatatcgg ataaagggtt
     9361 aatacttttg atttctctct gtaatttttt tagtgcagat aaattcaagt tttgttttct
     9421 agacacaaaa atgcgatctg ccatgacaag ttgcttaagg gcttgtggtt ctctttcaat
     9481 aagttcaaaa ctgtcactat caataacagt caataaacta tcgatatata ctcttcctct
     9541 tagttgaggc gctgttaaaa gtgtttgaat gattggttgg ggatctgcta tacctgtagt
     9601 ttcaatgatg atttgtgaaa ttggttgttg atgttcttct attgtttcca gtaaagaaat
     9661 caatattgct aataaatcac tgcgaagact gcaacacata catccattat ttagttgtaa
     9721 cacccgttcc tcgctatgaa ctaataattt gtgatcaatc ccaacttcac caaactcatt
     9781 ttcaattata ataatttctt ccctggggta gggtgagact tgcaaggctt gattgattag
     9841 cgttgttttt ccagccccta aaaacccaga aacgattgta actggtatgc ccattttgtc
     9901 acctttttac cacttagcta tttcacttct ttaaaaattg cccgtctgcg caattttggc
     9961 gaatactttt ttaattctaa ccgttcaggg ttattccgtt tatttttgct ggtgagatac
    10021 agacgttctc cagtttcgac acattccaaa ataatatttt gacgcataaa tcttcctcct
    10081 tcaatactta tcgatcttca agatatgctt gaatttgttg tgcatcttca aaagttaact
    10141 cgccagattc caattgtgaa actaaatttt ctttcatatc aacaggtatc tcccaataac
    10201 catcgataca ttgaaataac tcctcgtcat actcttcaaa aattggatag attcttaaaa
    10261 aatcatcaaa tgataagcct tggtcctcat agtaatcttt gactaatcca ttttcaattg
    10321 cctgttgggt cggtaaaccc gtttcagtaa taattcctct ctctaccatc gaacgataaa
    10381 aagtgcattt atctagcgat tgctgggtcg tttcaagata atactttaat ccttcttcaa
    10441 ctaaaggtgg gtaaataatt tccattttga ttccctccta aaagttatat attaaactat
    10501 tttgaagctc ctttaattac tttctttcca ttatagtacc cttttaaaga tacatgatgg
    10561 ctacgtctat aatcaccatt cgcttcgtca aatgaaattt ctggttttac tacagtttga
    10621 tgagttcttc ttaaccgttt cttcgcctta gatgtttttc ttgctggtac tgccatgata
    10681 tttcctcctt ttaccaactt gcttttttta ctccaggaat caacccttga tgggcaagtt
    10741 ctctaaactt aatacgggac ataccgaact ttcgcatata ccctcttgga cgaccatccg
    10801 tctgatcacg aagcctcaga cgattcggat ttgaatctct tggcaattta gcgagagcgg
    10861 tgcgatctcc tgcggctttt aattcctgac gtttatctgc atacttttca atcatcgcca
    10921 tttgtttctt tgctttagca attttcgact tttttgccat atacctttat actcctttta
    10981 ttttgattta gacacctcaa atcgtaatcg ttacgttttg acactgatac tttaccaaca
    11041 agagttgctc ttgtcaacaa taaataacat gaaataaaac tcatatgaat agataactat
    11101 ttgaaaacga atttatcggt acatagagat aatcaaatca tttaaaagaa tatgcagttt
    11161 aggctctgaa aaataagaat tcacttttat tagtccaatc aaacacaaat accgttttaa
    11221 gtagcttttc ctctacttaa aacggcaata tgaaagaaac tataactttt tattcaatct
    11281 tttatgttta actggtttat cgccaatttt ttaaaacttt gattattcct tatattataa
    11341 gaatagacat taagtgaact aattcaatca tactgattct ttcgtgtatt caaaattaga
    11401 tgctgtttaa taaaattatt ctttttgaaa aagaattata actatatccc aaaaattgta
    11461 ggttaattta aagcctattt catcatcgtg attgagcttc tttttcatag acgaatttct
    11521 cagatagttt agttttcttt atgagatttt tgtatatttc tgaccttcga tacaactcat
    11581 tatgagtacc ttgatcaact agttttcctt gatccatcac tacaatttta tttacctttt
    11641 caatagactt caagcgatgc gatataatta gtactgtctt atcttgtatt aaacgattta
    11701 aactttcctg aatacgtttt tcattttccg catctaaact ggcagtaatt tcatctaaaa
    11761 ttaaaattgg cgcatttttc aaaaaagccc gtgctatgga taggcgttgt cgctctcctc
    11821 cagaaagtga acttccgttt tctccgacca atgtatgata accgtcaggt agtttttcaa
    11881 taaagtcttc gcagttagct aaacgagctg cttttttcac ttcctcatct gacgcttgac
    11941 tatttccaat acgtatattt tccaaaatac ttgtattaaa caaagttacc tcttgaaaaa
    12001 caatagcgat tttgctaaac aaagaagctg gagatacacg tttaatatca tacccatcaa
    12061 ttaaaataca gccttcatca taatcataca gtctagacac taatttcaag atactagttt
    12121 tcccggaacc actagcacct actaacgcag tcacttctcc ttgtttagcc gtaaaagaga
    12181 tatgatctaa aattggggta ttgttatcat atgagaaaga gacatctcgc aactcaatgt
    12241 caaaagattt cagaggacta tctgatccct cttggatact ggtttccttc atagctctaa
    12301 ttcgctgtat tttaggagct aaataaaata tttcaagcac tgcttctttc atagaatcga
    12361 aggaatcctt tatttttatt gcggctagta agtaaccaac gacgtagagt atagtcactt
    12421 cacccgttaa cagtaagtga acacctacta gaatgactac tgctaaacta acatatgaga
    12481 aaatagagga taaggccatg actgaaaaag tacttaattc tacctttaaa tgaatccttt
    12541 cactctcttc catttttttg aaaagtctgt cttgaacttt tttagataga ttaaagctat
    12601 taatctcttg ctgcaattct atagtttctt gaaattcttc cgagttttct ctcaaagtat
    12661 cgtaatattt agtattggct ttcgtttggg atttctttga taacaagatt aagacaaaac
    12721 taaataacgt tggcaaaata acagctaatc ccattttgac attaccaacc aaaagcatca
    12781 ctgaaataaa agggaaaaac agagccatac cgccggcttt aggtattgca tgactcatcg
    12841 catgctcaat accttcaacg tcagacatga tagtttgaga taaatctgat aaattatgtt
    12901 ttgaaaaata ggataatggt aaatttgaca aatcgtctgc aatttgcact cttaaatgcg
    12961 cactttcttt ataagtacta ttatataagt tttcatactc cctatctaac aaccaataca
    13021 tgaaaagcaa agtaacaaac gatacggcaa aataaagcca acggggcttt gtattttcta
    13081 aaactaattc gtctatcagc atcattataa tgaatgctgg taccatatta atgcagtata
    13141 caaaaaaact agaaatagaa gctttagtta aagcttctgc gccctgatca cttaaagcaa
    13201 atttgttttt ataaaaactt ttcattgtca agcctccaat catttgcatt ctcataatta
    13261 gtgaccaatt gtttatacac ttgatgttta gccattaatt cattatgatt tccacgttct
    13321 actattttcc cttctgacaa gacaagaatt tcatctacag atttaatgct ggataatcga
    13381 tgagctatca taataactgt tttttctttc atcagatgtt gaaaagcctt ttgtagttca
    13441 tactcattat ctggatcaat agatgcactg gcttcatcca taataacaat ctttgcatct
    13501 tttaaaattg ctctggcaat agctatacgt tgtttttctc ctcctgacag ataaactcct
    13561 ttactaccaa ttaaggtatg ctctctttcg ggaaattttt ctaaaatcga ttcgcaaccg
    13621 gctaatctta gcgcgtcaaa tactttactt gctggcgctt gaggatttgc tatagaaacg
    13681 ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa agacaaaagc aattgtgtta
    13741 ataattgtgt cttttgaata gtgatttaaa ggcttaccac caattttaat ctgtccctta
    13801 tccacattgt agaaacctga aattagttta gctatcgtac tctttccgct tcctgatttt
    13861 cctactaatg cataaattct tttttgttct aaaactaagt tgaaattttc taaaacacac
    13921 tcttctttgt atgaaaagct gacattttca aatgcaatgt cacaattttt aatctgtgtc
    13981 tcgttcccat attttagttg gtcttgcttc atttctaaat aaagggagtc tagtgtttcg
    14041 actgcatatc ttgcttcaaa caagtgcata cttacataca ttattctcat gaaagaaaca
    14101 aaaataattc cacttaaaaa aaacaacatc aacaagtcaa cagctaatat cttgggagtg
    14161 gtaagagaag ttataaaaaa ggttatagga actatcaaaa tgcaaactat gccaaaaaat
    14221 agccattgaa aaagaacaaa aggtgttttc ccactcaaag aatacttata tgcaaactta
    14281 gcataatcgt taattgcttt attcaaggct ttaaatgatg ttactttagc accaaatatc
    14341 tttataactt gaattccgcg gatatattct accgtttcag agcttaacac atctaacgat
    14401 tcttgatatt tttgaataaa atttgtattc tctgtcattt tctttaacaa gaatacacca
    14461 attactaaca tggcgattag aaccaatcca attttaaagt taatgaaaaa gcctaaggca
    14521 actgataaaa caggtgttac tatggcttga gcactatctg gaatcaagtg agcaattgcc
    14581 atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa tatatcccga agaatttaaa
    14641 tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc ccttttttct caaattagtc
    14701 tctaagcgaa acccaaggat atgagaaaat aagccagaaa aaatataaaa taaagctcct
    14761 aaagttaacc aagcaacagt ttgaaccgca taactcttag ctaaatattc atctcctaca
    14821 ataattagag actgaagaaa tttgtaaata ctgtaatatc catagactgt taatacgaca
    14881 gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt tcattttagg gacataacta
    14941 aataattttt tgtatacttt cattttttcc tcccccgcat tcaatttctt ctcttattcg
    15001 tttacaactg ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa cagataaaag
    15061 gtaaactacg ttctattttt tgtatttttt taagactttt cttccaatca atacaccaat
    15121 catagataaa cttgctacaa ctaacactga aatgattccc catataccta ccgcatagtg
    15181 gatcattgca tccattgttg cagcagattg tcctttatct atccaatctt gacgaaaacg
    15241 atctgtaaag aaccatatag gaatccactg tccggcagca ctgcctaatt gcattagagc
    15301 ccaagacgca acttgaggaa ttgctcgatc atatccaaat acagatagaa taatatcagc
    15361 taagatcgcc atactaatcg aactgataaa ccaaggtaag tacgccccgc ccattaacgt
    15421 aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt ccttttctag ggtagttata
    15481 gctaatataa acaaatatga ttccagataa taatccaaaa atcccggggg atatgctgtg
    15541 accaaacgcc ccaccaatac tactaaccat tattgaaaaa aagtaaatta ctagggaaat
    15601 aactgtagct aaaccaataa aaatataatc ttttgttttc atatatattc tcctcaattc
    15661 tatttcgtta tgtcattaaa aatctgttct atcaaagttg aatcttctac tctaagtttt
    15721 atatcttttt ttattccacc attttttaaa taaata
//
LOCUS       DACDRK010000057        15670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDRK010000057 DACDRK010000000
VERSION     DACDRK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.057"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWJ77_001971"
     CDS             complement(14..706)
                     /locus_tag="IWJ77_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9453563.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWJ77_001972"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWJ77_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWJ77_001973"
     CDS             complement(1143..1712)
                     /locus_tag="IWJ77_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9453564.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWJ77_001974"
     CDS             complement(1786..2109)
                     /locus_tag="IWJ77_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453565.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWJ77_001975"
     CDS             complement(2263..3075)
                     /locus_tag="IWJ77_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453566.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWJ77_001976"
     CDS             3429..4277
                     /locus_tag="IWJ77_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9453567.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IWJ77_001977"
     CDS             4422..5363
                     /locus_tag="IWJ77_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9453568.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWJ77_001978"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWJ77_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IWJ77_001979"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IWJ77_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9453569.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IWJ77_001980"
     CDS             complement(9827..12013)
                     /locus_tag="IWJ77_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9453570.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IWJ77_001981"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IWJ77_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9453571.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IWJ77_001982"
     CDS             complement(12235..12675)
                     /locus_tag="IWJ77_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9453572.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IWJ77_001983"
     CDS             complement(12749..13288)
                     /locus_tag="IWJ77_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP9453573.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13903..15369)
                     /locus_tag="IWJ77_001984"
     CDS             complement(13903..15369)
                     /locus_tag="IWJ77_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9453574.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4471 a         3263 c         2759 g         5177 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca gccacggcaa
    10381 tccacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccattaa tcgtaccgct gatcccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 ttgaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggggtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg acttccatgt
    11101 gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca taaccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacgt cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt agcttatgaa
    12721 tacagattac gcttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctctgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaaca atccatgacg gattagatac cataaacgat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataaat ggtgttcaat cagctgacct tcagcattaa ctcaaaaaac
    13441 ggcaaaatat gaggagctat tttttcgttt gttcctctaa tactcaggtc aaatcgctga
    13501 ttttacaacc tcttccttaa acggtgttct cctgccaaca cctcggtcac aaggcacaat
    13561 agtcaacaaa catgagaagt tgttttttga aattgctcct tgtgactcgg agttaaacgg
    13621 caggctcact acctctttct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    13681 agcaaaacga aaaatatgag gggctatttt tgtttggctg ttcttattac ttgatgcagg
    13741 acagcttttt cacaacctct gattcacggt gttccagaag tagcttctgc gaaaaacaag
    13801 ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    13861 tgaccacttc tgtcacaacc tcttgtaaca agtatttcta ttttaaggct gaaccactgt
    13921 ttctggttca gtgatttctt tgtttttgtc tcgacgagca attgctggca agatcatacc
    13981 taataagatc aagacaattg gtgtgatgat attcgtgaat aattggaacc accatgcttt
    14041 aggatcagca gtataatcta cttttggcac cattcctagg atacatgcaa acgcagtgaa
    14101 ggcaaagcac caagcaccaa agataaaacc gatttttgga tttttaacaa atttatattc
    14161 agaattgaat ttcttataag ctttattcaa catcatatat gccaagaaca cccataggta
    14221 acgcattggc atgacaactg agttcaagtt cgtcaaccat tttactaatt cattcatatc
    14281 tttgatacca aagatcggca acaaaataat gattcctact agtatacctg tcagaagata
    14341 accattcacc aatgtacctt tacgattacg tttacgtagc cagtttggta caaattctgg
    14401 atccgcatct gctaacaaga tttttagtgg tgcatcaatt gaaaaggcta atgcggagat
    14461 ttgtccaatc atgtttgtca atgcgtaaat gatcatcaat gagttaccga taccatagta
    14521 attacctaaa atttgaaaag cactgtatgc cccatttgcc atcaagtctt taggaatatt
    14581 atcgctagca aacagcatac ccattgcgac tgagcctaat acagcacaaa tccctaccat
    14641 tgctgctaag aagatcatcc ctttaggaaa ttcctttgca ggatttcttg tagagttgac
    14701 ataaggggat attttttccg ctcctccaac tgcaaatact agcatcgaaa cggttgtgaa
    14761 ataactgaaa tcaaatgttg gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa
    14821 ttccatcgtt ggattgatga acggtgccgt tacagctaaa agaataaata agatcgacat
    14881 gacgaacatc gctgttccgg ccagtccgcc aatcactttc aaagtagaaa gtccttttgt
    14941 tgataaatat aaaaaggcaa gaaatataat aagtgaaata actgctacca tttgtactga
    15001 catcgtattt acgatatttc catttccttg gactgcccaa ccgccagcaa tcaggattgc
    15061 ttgaggcttt tgtgccaaat aaggaatatg gacgacccag taagtccaag cggcatagta
    15121 tgccagtcgt ttattagatg tcgtattttc gacccatgaa ctgacgcctc ctttactatc
    15181 cttgaaggct gaccctaatt gtccaacgat caatgcgtat ggaataaaat aaatggccaa
    15241 aatcaatagc caagaggtga caactgatac tccttgctga gcatagttat taactacatt
    15301 ccccatgccc caaaccatgt taaatgcaat taacgcaaca gtaaaccagc gtaattgttt
    15361 gttgttcatt cgttctctcc tttatgataa aaatttgtta tccagatgta atcatattca
    15421 atttttaaca aacttacaag acagtgttat tttatgtgtg tattctttta tttaatacac
    15481 atcgtttttt catatttttt ccatcaaaaa aataagttat tttcttttat ttcatttacc
    15541 ttattgaatt tcaagctatt ttgaattttc tgcaaaatta aaaacattag ttattttata
    15601 atcctatcta ttttctttac atttttcatt aagaaaagga tataaaaaaa agaggacgtt
    15661 cattcgtcct
//
LOCUS       DACDRK010000058        15665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDRK010000058 DACDRK010000000
VERSION     DACDRK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.058"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="IWJ77_001985"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="IWJ77_001985"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9453575.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="IWJ77_001986"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="IWJ77_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9453576.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="IWJ77_001987"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="IWJ77_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9453577.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="IWJ77_001988"
     CDS             2856..3731
                     /locus_tag="IWJ77_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9453578.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWJ77_001989"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWJ77_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9453579.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="IWJ77_001990"
     CDS             4628..5062
                     /locus_tag="IWJ77_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9453580.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="IWJ77_001991"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="IWJ77_001991"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9453581.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="IWJ77_001992"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="IWJ77_001992"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9453582.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="IWJ77_001993"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="IWJ77_001993"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9453583.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="IWJ77_001994"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="IWJ77_001994"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9453584.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="IWJ77_001995"
     CDS             13333..14259
                     /locus_tag="IWJ77_001995"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9453585.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="IWJ77_001996"
     CDS             14441..15334
                     /locus_tag="IWJ77_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9453586.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="IWJ77_001997"
     CDS             15468..15593
                     /locus_tag="IWJ77_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453587.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
BASE COUNT         5519 a         2689 c         3358 g         4099 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatct
//
LOCUS       DACDRK010000059        15546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDRK010000059 DACDRK010000000
VERSION     DACDRK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.059"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(28..2109)
                     /gene="cadA"
                     /locus_tag="IWJ77_001998"
     CDS             complement(28..2109)
                     /gene="cadA"
                     /locus_tag="IWJ77_001998"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9453588.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(2251..2430)
                     /locus_tag="IWJ77_001999"
                     /pseudo
     CDS             complement(2251..2430)
                     /locus_tag="IWJ77_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(2633..2992)
                     /locus_tag="IWJ77_002000"
     CDS             complement(2633..2992)
                     /locus_tag="IWJ77_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9453589.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3022..3363)
                     /locus_tag="IWJ77_002001"
     CDS             complement(3022..3363)
                     /locus_tag="IWJ77_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453590.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(3360..4034)
                     /locus_tag="IWJ77_002002"
     CDS             complement(3360..4034)
                     /locus_tag="IWJ77_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9453591.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5211..6509)
                     /gene="asnS"
                     /locus_tag="IWJ77_002003"
     CDS             complement(5211..6509)
                     /gene="asnS"
                     /locus_tag="IWJ77_002003"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9453592.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6549..7739)
                     /locus_tag="IWJ77_002004"
     CDS             complement(6549..7739)
                     /locus_tag="IWJ77_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9453593.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7760..8287)
                     /locus_tag="IWJ77_002005"
     CDS             complement(7760..8287)
                     /locus_tag="IWJ77_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9453594.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8334..11123)
                     /locus_tag="IWJ77_002006"
     CDS             complement(8334..11123)
                     /locus_tag="IWJ77_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9453595.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLISTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11270..11470)
                     /locus_tag="IWJ77_002007"
     CDS             complement(11270..11470)
                     /locus_tag="IWJ77_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9453596.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11922..12242)
                     /locus_tag="IWJ77_002008"
     CDS             complement(11922..12242)
                     /locus_tag="IWJ77_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453597.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12626..13744)
                     /locus_tag="IWJ77_002009"
     CDS             complement(12626..13744)
                     /locus_tag="IWJ77_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453598.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14215..15522
                     /locus_tag="IWJ77_002010"
     CDS             14215..15522
                     /locus_tag="IWJ77_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9453599.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
BASE COUNT         4414 a         3334 c         2738 g         5060 t
ORIGIN      
        1 aggacgttca ttcgtcctct tcattgatca acgtttattt tttacatgca agcttcgcat
       61 ggcattcaaa acagctaata ctgtcacccc aacatctgca aatactgcat ctcccattga
      121 tgcaaaacca aaagcaccta aggcaagtac caaaattttt atcccaatag caaagacaat
      181 gttctgtttg acgattttca atgttttctt tgctaaatgg attgcttctg caatcttcga
      241 tggttgatca ttcatgatga ctacatctgc tgcttcaatt gcagcatcgc ttcccaatcc
      301 acccattgcg attcctacat ctgctcttgc tagcaccggt gcatcgttga ttccatctcc
      361 gacaaatgcg gtctttttat tgtttctttg taagatttcc tctaaccgct gcactttatc
      421 ttctggaagc aattcgctat atatcttgtc cacgcctact tgcttcccga tatgatcagc
      481 aatttttttc gaatcgcccg tcaacatgac tgtgttcttc acaccagcct tttttaattg
      541 aaccaatgcg tctacggcat cttctttcaa ggtatctgca ataacaagat aaccactgta
      601 actttgatcg atcgctagat aaagcaatgt accgatttct tgacttgaag gaaaagagat
      661 ttcaaatctt tccatcaatt tgtgattacc tgctaatact tgtttgccat caacttcagc
      721 taagatccct tcacctgcaa attcttggat attagctgtt ggtaaaagcg gacctgcgta
      781 gccttctaaa acagattgag caatcggatg tgtagaaaac tgttctgcac tcgcaacata
      841 ttgcatgaat atctttggat cgatttttgt atcgatcgtc tggatcgaaa agtcaccttt
      901 cgttaatgtg ccggttttat cgaataccac tgtctcagtt tgtgcaagca actccaagta
      961 atttgaacct tttactaata caccgatttt acttgctcct ccgataccgc caaagaaact
     1021 aaggggtacg gagatcacta atgcacatgg acatgagatc actaaaaatg tcaatgctcg
     1081 atagatccat tcagaaaacg ccgctccaga aacaacaagt ggaggtaaaa cagctaataa
     1141 aacggccaat cctacaacga ctggtgtgta atagcgcgcg aatttagtaa tgaaattttc
     1201 agctggtgct tttttactgc tggcattttc tactaattct aataatttgc tgatagtaga
     1261 attttcataa gatttttcta cttgaactaa aaatggttta tttttattga taaatccact
     1321 taatactgtt tcaccaacat atacagattt agggactgat tctcctgtaa gtgctgacgt
     1381 atcgactaat gattctcctt cgatgatcgt tccatctaac ggtacacgtt caccaggctt
     1441 cacaagtaca aactgaccga tctgcacgtc ttcagggttc acggtcatca atccttccgt
     1501 cgtctgcacg tgagctgttt ctggacggat cgccatcaat tcgcgaatcg attttcttga
     1561 atgactgact gccaatcctt ggaaatactc gccgatttga taaaatagca tcactgctac
     1621 ggcttctgga tattcaccga tcaacatagc gccaatcgta gcaacagaca taagaaaatt
     1681 ctcatcaaag atttttcctt tgacgatatt catcactgcc ttcttcacta catcatatcc
     1741 tatcagcaga taaatcaata aataccctat caagcgaatt ggttcattaa aatcaaataa
     1801 tagcaataaa gcgatacctg caagggaaaa taaaatccgg aacagcgtat ttctctcttt
     1861 ttcatttcca tgttcagata ctttttcctt atctatttct gttactttga catctggttc
     1921 taattccttc actacttttt ttatttcttg ttcaatcgta gccaaatgat cttcctctgc
     1981 gtcaatcgtt agttttccag aagtgaagtt taccgttgct tccttgactc cattgatctt
     2041 ctgaactcct ttttcgattt tcattgcaca gttcgcacaa tctaaacctt ctaatctgta
     2101 acttttcatt ttaatgacct cctcgcctca acatatgaat atctcttcat aactaattat
     2161 aaatgaacgg ttattcatat gtcaaggtat ccgaaagaaa aaatgaaaaa catctgcatc
     2221 ggaaaatttc ttccatacag atgttttggc ttattctttc tttttctttt cgatcattgc
     2281 cactgctctt atccaacccg cacttgcctt cttgatcttg acaggatagc aactaagttt
     2341 gaagcctgtt gctggcaatt tgtctaaatt cattaatttt tcgataggac aatatctttt
     2401 ttcaatccct gcaaaatgtc cttcccagat attcgaagct ccactacgct ttcaaaaata
     2461 tagaaagagt tctttaatga aaattatttc aactttccat aaacagtaac cccatataaa
     2521 taaaagacgt aaaagtcaca tggaagaact tatacaggag aacgcataga gtcaggtgaa
     2581 atacatgtat cccacctgac tctatgcttt atctaacctt tattattctc ttttatacaa
     2641 tttctgcacc gaatggcatc aacgccaatc gagcaagttt gaaagattgc ttggcaaaag
     2701 gtatccctat gatcgtgata catagaagga tgccgctgac gacatgtgcg agagcaattg
     2761 gtaggcctga aacgatcagc cagatgatat taacaatcaa tgacatgcca gactggtgat
     2821 aaattacttc tttgccaaaa ggcgcaaaag aaagggaggc gatcttaaaa cattgcatac
     2881 cgattgggat acctatgatc gtgatacacc aaagtatccc cgctgctaac cagctaagac
     2941 caccgattag tccgccgaat ataaaccaga tgacatttcc taaacaactc attgtatttc
     3001 tccttctatt actgtttttg attaaaactg ggcaaaaaat gattttattc ttcttttttt
     3061 agcttccagt atcaaatatt ctgcaaattt tttttggatt tcttccgtat tttcttttgt
     3121 tactaatgtg tgttggatta gttttcctga tggtcgctga tccaatcgtt cttctgactg
     3181 ttgcacgtcc gcttgcgcaa atccgataat ccgtttttct tttgttacag caacgagcaa
     3241 tagtttattc ttatcattta ggtcagctgc cgtttttgct tttacgaatg attctggatg
     3301 atcagtaatc agctgattga tttgaggtag atcttctttt actgcccacc gtatttccat
     3361 catttctcac ttttctttcg ctcattttta tctccactaa aaaaatcgac tgcggcaatt
     3421 gcatctatcc agctttgtgc aatgatctca tgtccgccaa tcgttgggtg gaccccatct
     3481 tctcttgtat atttcgaaaa accataaaca atccctaaag cattgaatat accatctaaa
     3541 ggaaccagca atgcgccata ttccttagca agtttacgaa caatttggat tttagggtct
     3601 aagtctttac gccagccatg acggtcgtcg gttataggaa gtacaaacgg ctccatcaag
     3661 atgatgttgc agattcccac tgtttgaagt tcatttagta attttcgata aacggattca
     3721 aattgctgag cagatttctc agttccgaat atagcctgat cgctagtatt atgccaggta
     3781 tcattgattc cgatcaaaat cgatacatag tcaggttgta aagctaaaca atcttcttgc
     3841 cagcgtactt gcagatctgc aattttgtct cctcctatcc cacggttcat gcattcaaat
     3901 tccatcgttg gatattggga gattagtttt ccggcaatca aaagtggata acctttacct
     3961 agatcagttg cttctgctcg gtctcttcct gcgtctgtaa tgctatcacc gataaataat
     4021 atacgttttt tcatttgtca aaaactcctt tttgaataaa acgatgattt tcttccttat
     4081 attatcccat aagccctatc aagacgaaag agaaaatata tgtataaaaa atgacaccag
     4141 cctagtccct tcaaacaatt gaatgaacgc tcatcaagtg tctagttttt tatgctattt
     4201 ttttcttaac gtgccggatt ccccttgatt ccttcgttgt ttggtccaga gagatcgatc
     4261 acacctaagc ttccgtcaat cagaccactg atgacgccat cagaacatat atttataggc
     4321 tcatctaaga cttctagtaa atgtcccttt actaatatct cttctagatt gcttgggata
     4381 gattggtcat aactttatct ggtccgatga tttcaagggt gaattgaact tacatatgga
     4441 ttattgcatg ctgaaaatac ggacctactc catcttcgaa tgttactatt agtttgtttt
     4501 caggtgctag atgctgcttt atttcaacag cagaagcatc atcaaatcag atttccattt
     4561 gaattacctt ctttgtaaat gtttgattga ttgcgatttc gttatagaac cttgtgaatc
     4621 aaatagcaat ttcttttgct ttcttctctt gtctgctgga aacttccttt atcccgtatt
     4681 ttaaaaaaag aactgcgaga gttcgcagtt cttttttaga ataaacggtg ctctccggcc
     4741 aactcctcgg tcacaagtca caatagtcat caaacatgaa aaactgtttt ttgaaattac
     4801 tccttgtgac tcggagctga acggcaggcg cacaacctct tgatataaac ggtgttcaat
     4861 cagctgaccc tcgatattag ctaaaaaaac ggtaaaatat gagaagctat ttttccgttt
     4921 tgtttctcta atactcaggt caaggcactg atttcacaac ctcttcctta aacggtgttc
     4981 aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtggaaag ccatttccac
     5041 aaaatttttc ttaatactcg aaactaaaca gctttttcac aacctctcca ctaaacggta
     5101 ttcaaaagct tacatctgcg gtaataagtc ccgccaattc aaaaacatga acagctgttt
     5161 ttgcttggct gttctaatta cttgatgcag aacagctttt tcatcacctc ttatggataa
     5221 attcggttca acaaacgtgg gaatggaata gcttcacgca catggtcgat accagcaatc
     5281 caagtgactg ttcgttcaag gcctagacca aaaccagagt gcggaacaga accgtattta
     5341 cgtagatcaa gataccatga atattctttt tcatccaaac cgtgattacg gatttgttct
     5401 agcaagaaat catagtccgt tgcccgttcg cttccgccaa taatttcacc gtaaccttct
     5461 ggtgcgatca tatctgcaca aattaccacg tcttctcttg ttggatgtgg tttcatgtag
     5521 aatggcttga tagcttttgg atagtttagg ataaagacag gctgttcgaa tgaattcgca
     5581 atgaacgttt catgaggtga accaaagtct tcgccccatt cgatgtcatc aaatccattt
     5641 ttcttcagta attcgatcgc atcatcataa gtaatacgag ggaatggcaa ttttgtataa
     5701 gtttttaaga cttctttgtc tcgtcctagt acatctaatg cgtattcgca atgatctaaa
     5761 acattttgta ctaagaatgc tacatattgt tcctgtactt ctaaactttc ttcttgatga
     5821 gtgaaagcca tttctggttc gatcatccag aattcaatca aatgacgtcg tgtttttgat
     5881 ttttcagcac ggaaagtcgg accgaatgaa aagacttttc cgaaagccat agcagcagct
     5941 tccatgtata attggccact ttgagataaa taagcttttt gatcaaagta atttgtttcg
     6001 aatagatccg tagtgccttc tggtgcggaa ccagtcaaga tcggcggatc gattttgaca
     6061 aaatgatgtt gattaaagaa ttcatacgtt gcacgaatga tttcatttct gatctgcata
     6121 atcgcatgtt gtcttgaaga acgtagccat aaatgacgat gatccatcaa gaagtctgtt
     6181 ccgtgttctt ttggtgtgat cggataatcg tggctctcgc ttaccacttc cacatttgta
     6241 attccgattt cataaccaaa ttttgaacgg ctgtcttcac ggatttctcc agttactaac
     6301 acggatgttt cttggtgcag atttttagct gtttggaaaa cttcttccgg cacttcgctt
     6361 tttacaacta caccttggaa aaaggctgtt ccgtctctta attgtaaaaa tgcgattttt
     6421 ccgcttgatc gcttatttgc gacccaagcg ccgattgtta ctgtttctcc cacatggttt
     6481 ttcgaatcga tgatttgaat ttgttccacg tatgactctc cttttatatt ccttgaattt
     6541 cttcaggatt aattttgact tttactctca ataaattgct tgattctgcg taccgcttct
     6601 tctaacgtag cgcgatcagt ggcataactc atgcggacat tttctggtgc cccaaagcct
     6661 tcgccagtca caagcgctac ccctgtttct tctaaaagat cttctaccca ttgtgtgaca
     6721 ttttcatatt tacacatgcg tagcgtttct tttacattag gaaaaagata aaaagctcct
     6781 tgtggctttt caagagaaac acccggcagt tctgccacaa gcggatataa cgtattcagg
     6841 cgttcttcaa aggcgttccg catttcttcc actgtatctt gctcgccttt caatgcttca
     6901 acagctgcat attgactgac tgctgtcgga ttgctagttg attgggaagc aacagcgatc
     6961 attccattga tgatcacagg atttcccacc gcataaccaa tgcgccagcc tgtcatcgca
     7021 tatgttttag acacgccgtt gatgatgatg gtctgatttt tgattgcttc tgaaatcgtt
     7081 gcaattggag taaactcatt gccattataa actaaacggc cataaatatc atcagcaact
     7141 atcaaaatat cattttttac tgcccattca ccgattgcct gcagttcatt cttgctataa
     7201 atcattccag tcggattcga tggagaattg atgattacgg cttttgtttt ttctgaacgt
     7261 gcttgttcta gctgctcaac agtaactttc cagctattgg attcttctcc tttcaaaaag
     7321 acaggaagtc cttctgccag ttttacttgc tcaccgtaac tgacccagta aggcacagga
     7381 atgatgactt catcttgcgg gtcaaggatc gtttggaaaa gggtatacaa agcaaatttc
     7441 gctccgtctg ttacgactgt ttgtgaaggt tcatagacaa gtccatagtt tttcttcaga
     7501 taatcaacga ttgcctgtcg aagctctggg attcctgctg ttggtgtata atagctagct
     7561 ttcccattgc ggattgcttc gattgctgct tcttggatgt tttctggtgt cgcaaaatcc
     7621 ggttctccca cagtcaaact cagcacatct ctgcctttgg cttttaatgc cttggccttt
     7681 gccgcagctg ccaaagtgac agacggttct aatcgttttg ttctttttga taactccatc
     7741 ccattcattc ctttaccttt taaattccct tgattgtctt gacttcatct ccagattcaa
     7801 agccaatcag gtaataattc agtttcccat ttgtttcttt tcctactact tcccacacag
     7861 gctgatcatc atacatccct aaagcagctt tttcgatttg gatttcagga tgggccgcag
     7921 caattgtctg ttttgcttca ttttcagtca gtccctttgt ttgatccaat acttttactt
     7981 tttcaccaga tttaggaatg ataacggcga tttcttttcc tttatcattt gtacctgtca
     8041 gtgaaaagta tgtcttcttt cgagtaaacc aataaaaatg atcggcttcc tcaagatttg
     8101 catatttctt tgcgatttct actgcttctt tcttcgcctg caccgccgga cgactggaac
     8161 gcaagtaaaa aatcgagctg atcacgatga ccgctaataa aaatacgatc aagccaagta
     8221 acacgcgatt aatcgttttt tcctttgtct gttttttctt caagggtcgt cgctccttta
     8281 tcttcattcc ttttcattat agcagatttt aattatgaaa aaagacagct cctctaactt
     8341 ccttcttcat ttttctttaa gaattcatgg atatcctgat agatttcctc catagctagc
     8401 tctttgatcg gtactttttt cggtaatgca tggatgatcc gtgcaccata ttttgcagta
     8461 actagtcttc gatccaaaat aagcaatacg ccacgatctg tctccgtact tatcaagcgt
     8521 cctaatgctt ggcgtaactt caaagctgct ttaggtacgg attcctggta aaaaggattg
     8581 atcccctctg attctaaata agcgttccta gctttgacca tcgggcgaag cggattatcg
     8641 aagggtaaac gtgtgactac aacgatctgc aaagcatctc caggcaaatc tacaccttcc
     8701 caaaaactgt cagcgccaaa caatacgctt ttttctgaca aaatgaagcg tttcaaaagc
     8761 ttctcccggc ttccgccaaa cccttgagct agtatttctc gtcctttttc tagcatagac
     8821 agatgcattt tctgatagac cttctgcaaa acatcatgag aagtgaataa cacaagtatt
     8881 ggccgatctt cttgttcgat cagacctgta agtgctgtct ctaaataatc gatatatgct
     8941 tgattgctga cttgatggac ggcaggtgcc tcttttggca catagatcct tgcctgtttc
     9001 tcataatcat aaggagaggc aatcacttta agaggtgctg cttcaatgcc aagccgctta
     9061 gagagatagt gtcgattagg gccgatcttc agcgttcctc caatataaag aatacgatca
     9121 tatcgaggat accaaacagt cgacggcaac atagccgctt gaaaatcatg gagatgaaaa
     9181 atcatctgct gcgctgtgtt cccaggaacg aaccaatgga tcaaatgatc tcgccagtct
     9241 tccagccatt gatccatcag caagtactgc ttgtctaatt gatcaaataa gctatataaa
     9301 cttgctaaat cgatccgcat ctggtttgtc cagctttctt ggataatctc aaaagtcaga
     9361 cgcatttgtg actgtagctg ctggatctcc tgataaaaaa gctgcaggtg atcaatggct
     9421 gtcccagatt ccagtggcag ctgatccaac atctcttttg tcgtcatgac ttcttctgga
     9481 tactgtccgt taggttgctt gccgctcact aaactcatta atccttctgc catacaaatc
     9541 tgtgcttcgc atagcgcaga aagttcttct cgataaatcg ataaccagcg taaggcttca
     9601 tgttctttcg gtaaatagct ttgtattcgg tcaaacagct gatttggttc ttctaattgc
     9661 tggatttgtt tatggaaata gttaatactt aatcgtttgt catttacttt ttcagcgatt
     9721 tccggcaaat gatgtacttc atcgatcaac aaaaatggac tagctggaag caatggttct
     9781 tttcgctgtg tttcttgtat taaaaacgca tgattgacaa tcaaaaaatt actttgcctg
     9841 attttctttt gcaagaaacg aacaaagtct tgttcgtaaa atgactgatt tcttgctaaa
     9901 aatccagtgc ctctgtgcgc gatatctgca aagaatgaat ggttcaaatt tgttaaatgt
     9961 aattcatcca aatcaccggt cttcgtttgc gtcagccaaa ctaagaccgc catttgatac
    10021 aaagcatatt gtttttgttc tggcggttcc acaagtgtgc ctttaaaacg ctgtaaatca
    10081 atatagtggc gatagctttt gataattaca ggttgtatgg gctgatctag caattgattc
    10141 agcaatggaa tgtctttttc tatgatctgc tgctgcaaaa ctagcgaaac ggtactgatg
    10201 acagcgggtt tttcaggtgt agccaaataa ctcaatggca gcagataacc gattgtttta
    10261 cccattcctg tagctgcttc gatcatcaaa tcttttgatt catcttttgt gaaatggtcg
    10321 tagaccgcat tcatcagtct gttctgttct tttcgataca tcagtgtttt accaaacatt
    10381 ttttcttttg cttgtttttt tcttggatat gtccgctcgc cataatggac gtatatgaat
    10441 agttccactt cttttttctg caaagctagc ccatcgacga tttctaatga gggatcaagt
    10501 ggctctggtt tttctttcat ttcttcaatc acatgataaa tgaaacgact cgtatccata
    10561 cctgtgtgtc tacttaatcg cgcaatctgc tctaacgtga tgattggcaa ttttctcatc
    10621 cgttcttcga tcagcaaaag aagttgaccc gtcacttcgg catcactatc tgcctgatga
    10681 ggattctcat gaataaatcc aaggctttct gacaaatcag ctaagcgaaa gcttggttcc
    10741 gtcggcaaga aaatttgtgc aagttctact gtatcgatgc ctggtatctt caaactagga
    10801 agtccgcatc gcatgagttc atggttcaaa aaattataat caaaataaat attatgcgcg
    10861 acaaatatgg tgtctgccaa aagattgtaa atcgtttggg cgacatcttc gaaataaggc
    10921 gctttttgga cgcgttgatt cgtgatccct gttaaatgct ggatttgttt tgaaatcttc
    10981 cgccctggat gaatatcgat cgaaaaacgt gaagtgatcc gacctgattc aatcatgacg
    11041 cagccaaatt ggatgatccg atcttctttt ggattcgtcc cagtcgtttc gatatcaacg
    11101 actgcataag ttcttggttt caaaatatct tcctcttctc ctgcctctaa tgttcttttg
    11161 aaattatact acaaagtgca gaaaaatact ctcatcgctt tttaaagaaa atagacaaaa
    11221 acgcccagac acgcaaatgt ctgggcgttt attttttagt ataggttaat taagctttag
    11281 taacgtttgt tgcttgaggt ccgcgttggc cttcttcaac atcgaaagtt actgcttggc
    11341 cttcttctaa agttttgaat ccgtcgcctt ggatagctga gaaatgaacg aatacatcgt
    11401 ttccgttttc tgcagtgatg aatccaaatc ctttttctga gttaaaccat tttactgtac
    11461 ctgtttccat aataaaaatc ctccttgtgt atcaacacat attttgcaat tacttgaatg
    11521 gtgtatatct ggaagatgtt tatttgaaac aagctaccac agattaccga acaaaaacta
    11581 ctcctatagt atatcatacc gtttacaaaa aagctagagt ttttcgactt tttttattaa
    11641 aaaaagtttt ctcagcgatt acataaagtg aaaaaccgaa ctttttttaa aataaacgac
    11701 atattgttca gttggtgttt ttttgctctg atgttcttct ttaaactaaa aaaattattc
    11761 tttctgacta cttatcggtt ctgtttcaac ggtcgaggct ggggaagatt ctttagtagg
    11821 tctgatgttc tcatggtatt tctgatagcc tatgacagtt accagaagaa caaggcaaac
    11881 caatacgatc aacgagatta gtttttcttt tttgctcata tctatacctc ataaataaca
    11941 ggatgatgtc catcatattt ctgagtatga gtaaaagcaa aagctgcagc tgtagaatgt
    12001 aagaaatgcc agcaactttc ataatcacag gtcggacagt accaaaagta accaaatctt
    12061 ggctgcaaag accgttcttc tgtttcagga atcgcagaat caagatctgt tgtctgtaca
    12121 taaacatttt tgtacagacg tgtgcctgta ataggagacc gctgtgtttc ttcagcaaaa
    12181 actggctgac ttgttaaact tagggataga aacagcaaac accataacag tctttttttc
    12241 ataaagacct ccttatcgat aagtttttcc caattaaaat catatctcaa gaacgcccgt
    12301 tattcacatt ttgttcataa atgatgtttt ttgtatcata atcgtcaaaa agaggtcgtg
    12361 accacgtcac aacctctttt ttaaacggtg ttccagaagt agcttcttcg gaaataagtc
    12421 gaaatttcac aaaaatttga gaaataattt ttgtcaatct cttcttattt cttgaagctg
    12481 accacttctg tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc tgcggtatta
    12541 gctcaaaaaa cggaaaaata tgtgaaacta tttttccgtt tgtttctcta atactcaggt
    12601 caaaacactg atttcacaac ctcttttaat aacgaagaat cgctgtcaga ctcttttcat
    12661 ctggcgcatc tttttgatcc aaaataaaca ctttcccgcc attaatgatc acatcatctg
    12721 ctaatgcatt caacacttga cgccgatcat attctgtttc aggatcttca ccgaaaccat
    12781 cagctaagtt tgacgtagcg ataaataaat gtgacacttt tccttctttc gcagctggaa
    12841 cgatatcaac taactgatcg atgaatttct tatctaagag tttattgtat tcttttgatt
    12901 cttgagctgc gagttcactt gacagcttct tcgccccttc ttcgatatct ctaaaggata
    12961 gctgcgcagg agaagcagaa atcgcaacgt ctgcacaata atagggattt ttagcaattt
    13021 ttttgaacat cgtctgattt tctggtaatg caaataaata aatcggccat tgttcaggat
    13081 tttcgaaaga atccttcaag tacgtatcaa cagcttggta atagttgacc caatcgatct
    13141 ctacttcttc atcttttgga tttacgccat ggaaagttac tccatctcgg gctccgcctc
    13201 ctccattaga agagaagttc aaaccgccac ctgtcagttc agatcccaat gcagtaacga
    13261 catcaaccgg tgccccttct ggtagagata cttcttttac ttgtttgttg ctgacctcat
    13321 acaatttcat ggagtcgcgg ttcaatccca ataaataata agaatagttg aactgcccat
    13381 ttttgataat agccagtaaa taaggaagat cacttacata gtattgatcg tctaccggga
    13441 tactcaaacg atggatatag gtctggtcag cagttataat gaccgctaca ctcgtagttg
    13501 cactacgcca aaacgaagga tctcctaaca aattttcaat tttttcttgg aacggggacc
    13561 agtcatgttc ggaatatttt ttctcaaaac gtttctttgc ttctttcgca aaatttttga
    13621 atttgatctg atctttttcc acatcttgat gggcgacatg cgtattcagc atgatcgtga
    13681 cgaatggtcc tttagcgcct tctgaagtca attgtgtcaa taattcttta tccttttttg
    13741 ccataaacgt acccctccta aaaaaatcat tcctttgttt ctatctaaag tctaccacaa
    13801 cactattttt ccatacaata aaacgtatct aaaaaaataa aacggtttct acggttttaa
    13861 atttagctat ttttttaagt tatcttcata atttcctcaa ttatttagcc ccagtcttca
    13921 actattcttc atgaaatgct gataaactaa gaaaaagaat aaaagaggtg gatcttatgc
    13981 atttgattat gattttcagt ttattaagta ttatcagttt atttgctttc agcgcttccc
    14041 gcttattaga tgcggaaaca cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa
    14101 cttccataac gcgcgttctt atcctaaagt tttttcagaa acattgaaac tctctcccct
    14161 ctctgataaa attttaattt gtagcgaaac aaattaatta caaggagatc ggatatgtcc
    14221 ataaaaaaaa cacgtctagt cctattattg gctagtctca ttctttgcct tgcttctttt
    14281 ctaccagctg ttgctgtgca agctgaagat acgttcaaag taaatgcaaa agcagctttt
    14341 gctgtagatg cgcaaagcgg taagattctt tatgatcaag atggagaaaa aaccatgggg
    14401 atcgcttcaa tcacaaaaat catcggtctt tacatcgtcc ttgatcaagt aaaagaagga
    14461 aaactgtcat gggatgataa agtttcgatt tcagattatg cagaaaattt gagtatcacc
    14521 ccagatttat cgaatgttcc tttgcataaa gaaaacacct acactgtcaa agaattattc
    14581 gactctgcaa tcatccagtc agccaatgct tcaatggtgg ccttggcaga aaaaatctct
    14641 ggcagcgaag ctaaattcac tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa
    14701 gatgcaacaa tcgttaatgc atcaggattg aacaactctt atcttggcga aaaccgacca
    14761 gaaggcactg gagaaaacga cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc
    14821 cgccatttga tcttggattt cccagaaata ctggatgtat caagtactac gacccaaatg
    14881 tttggcgaaa atacgcaatc accagtggaa atggtcaact ggaactggat gctcccagga
    14941 ttcgttaatt acaaggaagg cgtggacgga ttaaaaactg gtacgactga gcttgcaggt
    15001 gcttgtttcg ttggtacgat cactaaaaac gatcaacgga tcatcactgt cgtattgaat
    15061 gcggacggcc atgcggaaaa cccgagtgcc cgcttcaatg aaacagaccg tttgatggac
    15121 tactgctatg acaattggtc agaaaaagag ctaggaaaag ctaacgcaag tattcctagt
    15181 ctaaaaacca ttgatgtaaa agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc
    15241 cctgtaaaag tatgggtacg aaacgatatg gatactggca aattaacgat cacgccaaca
    15301 cttgacaaga agcaagtcac tgacaatgag atccaagcac ctatggataa aggtacaaaa
    15361 atcggtaccg ctacgatcac cttggcagac gataaattag gctatttaga agacaacaat
    15421 gcaccttcaa cagagatcat tacggataaa acagtggaaa aagcgaatat attcgtgatt
    15481 ggctggcgac gcgtcgcaga atttttcggc aacttgtttt aattgaacaa taagaaaaac
    15541 atcttt
//
LOCUS       DACDRK010000060        15320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDRK010000060 DACDRK010000000
VERSION     DACDRK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.060"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..516
                     /locus_tag="IWJ77_002011"
     CDS             <1..516
                     /locus_tag="IWJ77_002011"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9453600.1"
                     /translation="DNVVVYILGHGTKKELLENLIKAYQLQNVIFLLGGQSNPYKYMS
                     RADLYVMPSITEGFPNALVEAMSTETAVLCADCKSGPREILSNRALDTICENIEYAES
                     GILVCPMTDSRNYSPQYEECDKNLANAIEELIENDNLRNKYAKAAKKRAELYSYDAFE
                     HNLLQIMKTFN"
     gene            554..1645
                     /locus_tag="IWJ77_002012"
     CDS             554..1645
                     /locus_tag="IWJ77_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9453601.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(1713..3191)
                     /locus_tag="IWJ77_002013"
     CDS             complement(1713..3191)
                     /locus_tag="IWJ77_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9453602.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(3457..4205)
                     /locus_tag="IWJ77_002014"
                     /pseudo
     CDS             complement(3457..4205)
                     /locus_tag="IWJ77_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            4348..5298
                     /gene="pfkB"
                     /locus_tag="IWJ77_002015"
     CDS             4348..5298
                     /gene="pfkB"
                     /locus_tag="IWJ77_002015"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9453603.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            5280..6743
                     /locus_tag="IWJ77_002016"
     CDS             5280..6743
                     /locus_tag="IWJ77_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9453604.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            6740..7198
                     /locus_tag="IWJ77_002017"
     CDS             6740..7198
                     /locus_tag="IWJ77_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9453605.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            7195..8169
                     /gene="lacD"
                     /locus_tag="IWJ77_002018"
     CDS             7195..8169
                     /gene="lacD"
                     /locus_tag="IWJ77_002018"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9453606.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(8348..9298)
                     /gene="yidC"
                     /locus_tag="IWJ77_002019"
     CDS             complement(8348..9298)
                     /gene="yidC"
                     /locus_tag="IWJ77_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9453607.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(9736..10011)
                     /locus_tag="IWJ77_002020"
     CDS             complement(9736..10011)
                     /locus_tag="IWJ77_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9453608.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10119..10883
                     /locus_tag="IWJ77_002021"
     CDS             10119..10883
                     /locus_tag="IWJ77_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9453609.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            11006..11509
                     /locus_tag="IWJ77_002022"
     CDS             11006..11509
                     /locus_tag="IWJ77_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9453610.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            11917..12564
                     /locus_tag="IWJ77_002023"
     CDS             11917..12564
                     /locus_tag="IWJ77_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9453611.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            12727..13995
                     /locus_tag="IWJ77_002024"
     CDS             12727..13995
                     /locus_tag="IWJ77_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9453612.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(14022..15221)
                     /locus_tag="IWJ77_002025"
     CDS             complement(14022..15221)
                     /locus_tag="IWJ77_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9453613.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
BASE COUNT         4847 a         2601 c         3165 g         4707 t
ORIGIN      
        1 gataatgttg ttgtgtacat cttaggacat gggacaaaaa aagaattatt agaaaattta
       61 ataaaagcat atcaactaca aaacgtaata tttttgctag gtggccaatc aaatccttat
      121 aaatacatgt cacgtgcaga tttatatgtc atgccatcta taactgaagg ttttcctaat
      181 gcattagttg aagcaatgtc tacagaaact gctgtcttat gtgcagattg caaatctggg
      241 ccaagagaaa tattatcgaa tagagcgcta gacacgattt gtgaaaatat agaatatgct
      301 gaatcaggca tattagtatg tccaatgaca gacagtagaa attattcgcc ccaatatgaa
      361 gaatgtgata aaaatttagc gaatgctata gaagaattga ttgaaaacga taatttgagg
      421 aataagtatg caaaagcagc aaaaaagaga gcagaactat atagttatga tgcctttgaa
      481 cataacttgt tgcaaattat gaagacattt aattaattca tatgatgaaa taaaaataag
      541 ggagtataac tagatgctga aaaaaaatac atctttacag aataaaacaa tattaataac
      601 tggcgcagcc ggttttattg gatcaaattt ggttcttgag ttattacgta ctcagtcatc
      661 aataaaaatc attggcattg acaatatgaa tgattactat gatgtctcta ttaagaacta
      721 tagattatca gagatagaaa cggaggcaaa gaaccatcca ggatctactt ggaattttat
      781 caaaggttct attgctgata aagcaacaat tgatttcctc tttgataatt ataaaccaag
      841 tatcgtggtg aatttagcag ctcaggcagg agtccgttat tcaattacta atccagatat
      901 gtatatcgaa tctaacataa taggattcta taatattctt gaagcttgtc gtcattcata
      961 tgataatgga gaatctggtg tggaacattt agtgtatgcc tcatcgtctt ccgtttatgg
     1021 tactaataag aaaattcctt attctacaga ggataaagta gacaatcaag tatcgttgta
     1081 tgcagcaact aagaaaagta atgaatcaat ggcacatgca tattctaaac tttacaatat
     1141 tccgtctaca gggctacgtt tctttacagt atatggtcct gctggtcgtc cagatatggc
     1201 ttattttggt tttactgata aacttcgtgc tggtgataat atccaaatat tcaattatgg
     1261 gaattgcaag cgtgacttta cctatataga tgatattgtt gaaggagtaa agagggttat
     1321 gcagtgcgct ccagagaaaa ttgatgggga agatgggcta ccaatcccac catatcatat
     1381 ttataatatt ggtaacaata cgccggaaaa cctactagat tttgtagata ttctacaaca
     1441 agagctaatt gcagcagggg tgctaccaga agattacgac tttgaatctc ataccgaact
     1501 agttccaatg caaccaggag atgtgccagt tacttatgca gatactagtg cgttagaacg
     1561 agattttgaa tttaaaccaa gtacaagttt gcgtgacggg cttcgtaaat ttgcgcagtg
     1621 gtataagtct ttttatgaag tataattgta tttttttact tataaaagtt tatagaaaca
     1681 cctcagacac tacaaatctg aggtgttatt ttttaatctc gttgaaataa atctctggta
     1741 tatacttttt cttttacatc atcaagatta ctatcgtaac gattagcaat aatactgtga
     1801 ctgatatttt taaaatcatc taaattattt attacttttg aaccaaaaaa tgtagtgtta
     1861 ttttcaagtg ttggttcata aattacaata gttgccccct ttgctttaat tcgcttcata
     1921 actccttgaa tagatgactg acggaagttg tctgaattac tcttcattgt aagacggtat
     1981 acacctatga ctacctcttt ttctttttct gcattccacg aactgttagc ctcatacgtg
     2041 ccagcaagtt ctaacactcg atctgctata aagtcttttc ttgtacgatt actatcgaca
     2101 attgcactta tcatattctg aggaacatcg ttatagttag tgagaagttg ttttgtatct
     2161 ttcggtaaac aatagcctcc atatccaaaa gatggattat tataatgtgt gccaattctt
     2221 ggatctaagc aaaccccgtg aatgatttct tgtgtattta gtcctttcat ttctgcataa
     2281 gtatccagct cattgaagta agatactcga agtgcaaggt aagtattagc gaataattta
     2341 actgcttcag cttctgtgaa tcccatgaaa agagtttcga tatctttttt gattgctcct
     2401 tcttggagta aatctgcaaa agtgtgagag gcttctcgta aagttttgtc agtttggtcc
     2461 gtgccgacaa taattcgcga aggatataga ttgtcatata atgccttaga ttctctgaga
     2521 aattctgggc tgaatagaat atttttagta ttgtattttt tacgaatact ttcggtatag
     2581 cctacgggta ccgtcgactt gatgaccatt attgcctttg gattgacttc taacaccaat
     2641 ttgattactg actctacagc agaagtatca aaataatttt gtttactatc ataatttgtt
     2701 ggagcagcaa tgacaacaaa atcggcttct ctataggcgg ctgcaccatc agtcgttgca
     2761 attaaattaa gatttttttc agctagatat ttttcaatgt aatcgtcttg tattggagat
     2821 ttcttattgt taatttgttt tactttttca ggaagaatgt ctactgctgt tacctgattg
     2881 tgctgggcaa gaagagtagc aatggaaaga cctacgtaac cagtaccggc tacagcaata
     2941 ttcttcgact gatgttttgt tctgtttgat ttatttgatt catttataaa tagatctgtt
     3001 ggttcaaaat ttagaacttc tgctaatgat tctaattgtt gtattgacgg gataaaatcg
     3061 agagattcta ctcgactaat taaggcacgg ttgataccag tagctactgc aagttgttgt
     3121 tgtgtcattt tcagagattt tctagaatca ataatgattt gagctaaacg tttttgagat
     3181 aactttttca tgatgcactc ctttttttgt tgctaataat aacaaacaat tttttcttct
     3241 ataacagaat agcttattat taataaaact gtcaattttt ttattgatta aaaagatatt
     3301 ttttgatttt aagtattttc aattattttt tgttacttaa aataacttta tagattgtga
     3361 ataactttat acataaaaag tttacgtgag tccatacaat ataaagaata aaaaatgtga
     3421 tgttttaaac tgtaaccaac cataataaac tgcttctcaa ttctccctca tacgcaatac
     3481 ataggcgtca agcaggattc tcgcgatgat ctggagaggc aagcgtgaac gtacttcata
     3541 atcagggaaa taagaacctt ctgatttgaa acctacgaga tttatctcgc aataatcttt
     3601 gagtgtagag gaatgattgg ctgtaactag aatcgaacca atctctgcat catagcagtt
     3661 ctttgcggcg gtgacgagtt cttttgtctc gccgttcaaa gaaacaaaaa taacgctgtc
     3721 tttcttagtt agtttccgac tgatgctttt gataatctct ggatcagtat gcatttgaca
     3781 attttttcct gttaattgaa gttttacgag catctcttga ccaatcattt ctgaaaatcc
     3841 tcgggcaaag acaaaaaccc ggtcagtaaa tttgattctt tgtattgcat cttcaatgtt
     3901 cccgatttcc aacatattga tcgtacgtac cacttcttgt tcatttttta gaatggctcg
     3961 acggatacct ttgtctactt tatctaaaaa gaaaaattga tgttcttgtt ttctccttct
     4021 tttagatggt gcttaaacga agtaaatcct tcatagcctt ttttcttcat tgtccgcaca
     4081 atggtggctg ttgagacatt tgcttgttca cttagtttca cgatggagta gttagggatc
     4141 tctaggatgt ggtgttcgat aaagttccag aggtactgtt ctgtttcact taatttgtga
     4201 tccatattct ttccttcttt cactaaaagt tagttaaaaa atagcacgat gtgtttataa
     4261 atgtaaaggt tttctttgtg ttggaacgat taagaaaaca ttttctcttt cgcgtatttc
     4321 ataatagatt caaggaggtc attggttatg tctatctaca catgcacgat gaatttggca
     4381 attgatctat ttattgaaac agaggagatg catcctttta tggtaaaccg tacaaaggag
     4441 gatgacattc aggcaaacgg aaaaggagta aatgtgtcac tagtattaaa gatgctagat
     4501 ttcccaagta cggcattagg tttttctgct ggtttcacag gaaaatatat agaagactat
     4561 ttacagaaga aacaaatcca aacagagttt attgaagtgc ctggtatgac gcgtatcaat
     4621 gtatttacac aagtcaatca aacgagagaa gaatacaaat tagtgaatca aggacctgaa
     4681 ataccgcagg cagctgtcca taatttttta aatcaaataa gagcattaca agcagaggat
     4741 tatctttgtg tttctgggag tttaccaaga ggaatgtctc cgaaggtttt agtggaaatc
     4801 agtcggattt gtcaggaaaa ggagattcat ttgattattg atagcagtga tcaagaggtc
     4861 atggattgtt tgccctatcg accatttttg ctgaaaccga atgaggaaga actttcttct
     4921 tggttcggac gaaaaatggt tacagatgaa gattactttg tttatggaca acgactggta
     4981 gagtcaggtg cagaaaatgt tttgttatcc cttggtgaga aaggagcgat tctttttacg
     5041 aaagacaggg tgattcgagg aaactcgcca aaaggaaaag ttgtcaatac agcatgtgca
     5101 ggtgatgcca tgttaggcac ttttttagta ggatacatga atcgacgacc gcttgatgaa
     5161 acgttacgta aaagcatagc agcaggcagc tctactgctt ttcgtaaagg actgactgat
     5221 tttttggatg tggaggaatt aagtaaacaa atcaaaatca aggaagagag gttcgaaaga
     5281 tgggaaaata tcaactaatt gcagcaaccg gttgtccgac aggaattgca cacacctaca
     5341 tggcgcaaga ggcattagag caagcagcta gaaaaaaagg catcacgatc aaagtggaga
     5401 cccatggaca aattgggatc gagaatgaat taacggctga agaaatcaaa caggcagatg
     5461 ctgtcattat tgccgcagac aaagatgtcc attcagaaag atttgcggga aaacgagtca
     5521 ttgatgtttc tgtcagtaca ggaatcaagg atgcacaacg gctgattgac gaagcattgg
     5581 caggaaaagg gaccatctta gcagataatg aagctgttga tactttagaa tcagaaacga
     5641 taagtggtgc taattttggt cgcagcatct ataaaaattt aatgaatggc gtctctcata
     5701 tgttgccttt cgtggtagct ggcggagtat tgattgctat ttcatttgcc gtttggggaa
     5761 tctattcttt tgatccagac catgcacaat acaatgctac agcggcgatg ttaaaaacgg
     5821 ttggcgatgc agcaatgggg atgatggttc cagttttagc agcttatatt gcagaaggga
     5881 tcgcgaaacg tccagggtta gtcgttggat ttgttggtgg aatcgtggcc atgagtggcg
     5941 gtactggatt tttagtaggt attttagctg ggttcttagc aggttatttt atcttatttt
     6001 tgcaaaagct actgacaatt ttacctaaac aactagatgg cttgaaggca atcttcttat
     6061 atccagttat tggtgtagtg tttatcggca tcgtgatgtc tttgctggcg ggtccaatga
     6121 aggctataaa tgaaggaatg atgagctttt tagctggatt tgaacattcg aatccacttg
     6181 ttcttggcgt gattgttggt tgcatgtgtg cgtttgatat gggaggaccg attaataagg
     6241 cggcatatgt aacaggtaca gctcttttgg cacaaggaaa cacgactttt atggctggcg
     6301 tgtcagtagc ttgcattgca ccaccgttga ttactggttt tgcaacttta ttgtttggta
     6361 aatattttga tactaatgat cgtaatgcag gtttggttaa ttttatttta ggatcaactc
     6421 atattacgga gggagctatt ccgtttgctg cgaaagaccc gatccgagta ttaccaacga
     6481 tgatgttagg ctcatcaatt gctgctgtct tgacctatct gtttggtgtc caagtaccag
     6541 ccccacatgg cggtttttta gtgttacctg tagtgacggg aaaaatccaa tgggttacag
     6601 cgattcttat tggttcgatc gttggtggag tgatactagg tttgatccaa aagaaaagag
     6661 taactgataa agacaagaag atggataata aagcagcaaa tcgtacagtt atcgaagaag
     6721 gaagagcagg aggcttatca tgacaatcat tcatgaagag aacatttttt tacaacagcc
     6781 attagatagt caagaagaag catttaagtt tttagcaaag caaacattct gtttaggaat
     6841 cgctcatgac gaaaaagaag tatatcgaaa acttctagaa agagaaaaag aaggtactac
     6901 aggaataatg tcaggttttg ctatccctca tgcgaagtct caaacaatca acgaacctag
     6961 tatgattatc gtcacactga acaaaggaat tgaatggaac agtttagata gacagtcagt
     7021 tgattttatt tttgcgttat ttattccaga ttcagaagca ggaacgactc atttgaaatt
     7081 attgtctctc gttgcacgat cattgatgcg aaaagaggtt acagaacagt tgagaaaagc
     7141 aaaatcaaaa acagaaatag ccacaatatt aactaaagaa ataggtgaag atctatgaag
     7201 aagatgagta tagaaaagca ggaacgtttg aagcgattga cggatgaagc tggaattatt
     7261 agcgcgttag caatcgacca gcgtggtgct ttgaagaaaa tgatcaacca atataaagaa
     7321 gctacatcag aagacatcat ccaatttaaa cagatggttt ctcaatacct gactccctat
     7381 gcctcagcta ttttacttga tccagaatgc ggcctgcctg cagcagcgct tagagagaaa
     7441 gaatgtggct tgctgctagc ttatgaaaaa accggctatg atacgaaaat gccgggaaga
     7501 ttacctgatt tattgaatat ttggtcggtg aaaaggttaa aagaagctgg tgcggatgct
     7561 tgtaaatttt tattgtacta tgacgtggat gaaccagcag agattaatgt gcaaaaacaa
     7621 atagttatag aacggattgg ttcagaatgc gatgcagaag gcataccttt ctttttagag
     7681 atcctttctt atgatgcaac tattggcgat ccgtcctctc ttgcctatgc aaaagtaaaa
     7741 ccccataaag ttattgaagc catgcgagaa ttttcaaaag aacgttatca agtggatgta
     7801 ttgaaagtag aagtgccagt gaatatgaga tttgtggaag gatatgggca agagacttgt
     7861 tatagtcaag cagaggcatc cgcgtatttc tatgaacaat cgaaagtgac tgaacaaccg
     7921 tttatttttt taagtgcggg agtcaatgct acgttatttc aagatacatt acgttttgca
     7981 aaacaatcag gttcgacatt caatggtgtc ttatgtgggc gagcaacatg ggcgaaggga
     8041 gtggcacctt ttgttaccga aggcgaagaa aaagcgagta gttggatgtt agaagaagga
     8101 aaacaaaata ttaaggaatt aagtacacta ttaaaagaaa cagcaacacc tgtctacgtt
     8161 gatagataag aaaaagttgt ttcttcataa aaagatgctg ctgcaattag atagcgatca
     8221 atcgatgatt caaattatac attcacttaa ataaaaatat cgactagatt tctttttcaa
     8281 aaatcttggt cgatattttt aattgctaaa actgttaatg aatttttatt acactcttat
     8341 ttactatcta cttccgcttc tgctgctttc ctgcgttgcg gcctttgtta tttgtactgt
     8401 tttttggttg tttgatttgt tttgttgctt gtttgttgtc agctggtttt gctgattctg
     8461 tcacgtcttt tcttggagtg acgacttgtt ttggcgggtt ttgtttcaac tcttctgcca
     8521 cttgtgcttt caagcgtggt ttcatcataa cgtttgtgat gaatgtttgc agacagctga
     8581 acacaccacc gactacccag taaagtgtaa cgccggctgg cgcactgatc gacatgaaga
     8641 cgatcatagc aggagagacg atcagcatcg tacgcatcgt ctttttctgt tcttcaggaa
     8701 taccgattgt tgaaatatat ccttgaagca gataagcgac ccctgcaatg gccacaagca
     8761 ccatacttgg tgagcctaga tcgatgccgt aaaaagatga ttcacggata ccttctgtgt
     8821 agcgagccgt aaaatacaag gcagagaaga tcggcatttg gatcaataga ggcagacagc
     8881 cgattccgcc ggtcatactc aatccgtttt cgcgatatac ttgctgcatt tctgcttgtg
     8941 ctgccatttg ttcttcacgt gttgttgcag cttttagctt ttgttgtgct gcttcaactt
     9001 gtggttttaa cgcttgcatt ttttcagatt ggatcattgt ttttttcgat tgactgatac
     9061 ctagtggcaa gatgatgata cgaacgatca cagtcattgc aataactgcc cagccgtaag
     9121 accaattgaa attctcaact agatatgtaa tcgcttggcc cataggatgg acgagtactc
     9181 gataaacgat tcctgaagtg tcagggtttc cgtttgaatc catccgcaca cagccagata
     9241 aaaagagcag gatactgaca agtccggatc ccaataacca gtttttccat ttattcatag
     9301 aatacttcct cttctataat ttttacacaa acttaactat actaaaaaag cgcatggaat
     9361 tcaatcaact tcatagaaat ttcatataaa tattctttct ttttcgtagc gaaaaatttt
     9421 gaaaaaagac aaaaaatcaa gaaagaaata cttggaaaga aaagagtgat tccaagtgtt
     9481 tctttcttat ataaaaaact aaaaactttt ttgttctgtc tggaaaatat gggttaagca
     9541 aatttattgc aagttataga tcagcttttt tactacctat ctttaatact tgaggcaaag
     9601 cgcctcaaaa acaacctgat cgacggtgtt caaaagtcag tttctcggta ttaagtcaaa
     9661 tttatgcaaa aatgtggaaa gccattttca caaaattttc cttaatactc aaaactaagc
     9721 gaccttttca caaccttatc ccaccacatc aaatttcttt cgttcctcaa tcatcggatc
     9781 ttcttggaca tccacatact gcactcgtcc acttgggctc ggagacttct tgacttcttc
     9841 gataaatttt tggatgatgt cgtcatttcc gattgcttcg attgtgactg agccgtcgtc
     9901 ttcgtttttt accgttccgg tcacgcctaa ctgatcagca accatcttgg tcatatagcg
     9961 aaatcccacg ccctggacac gtccttggac attcatttta actttgcgca tgttcgtcac
    10021 ctttctttcc tgttttataa atcaatgata ccgatttttt taggatgaaa caagctcaag
    10081 cacctattcc atgatataat caggtgaggt gaaggaatgt gaaagagatc caatcagcaa
    10141 aaaatccaaa aatcaaagaa ttaaaaaagc tacataaaaa gaaatacaga gaagaaaagg
    10201 cagaatatct tttggaaggg ttccatctga tcgaagaagc tgcgtcgtct gatgcagaaa
    10261 taaaagaact ttttgtgaca cagagaggta tgaaagaatg gggagactgg ctggctgtaa
    10321 acaaagagat ttctactttt ctagtttcag atgaggtcat gaccgctatg tcagatctac
    10381 ctacaccgca agggatctta gcggtgatga cgttaacatc tgaagatacg ccagattttt
    10441 caggaggctg gcttttacta gataatgtac aggatcctgg aaatgtggga acgatgatcc
    10501 gtacggcaga tgcgtttggt atggctggtg ttttattagg taaaggaacg gcagatattt
    10561 atagtacaaa ggtgctgcga agcatgcagg gaagcaatta tcatttgcct gtgttacaaa
    10621 aagatttggc agaagtgatc cctgtaatga aaggcaaagg gtttacggtg tacggtacag
    10681 aattaaatga agaagcacat gcattagaca aagtagaaaa aacagaggat ttcgctataa
    10741 tcatgggaaa cgaaggtcag ggagtaagtc aggaaatctt gtctttgact gatcagaacg
    10801 tatatatccc aatgaaagga aatgcggaat cattgaatgt cggagtcgca gcagggatcg
    10861 ttctttatca tttttcttta tgattaagga actattaagc tttagctatt ttatctagag
    10921 cgactagaat aatccgaaca ctggtggtat acttagatca atttcagaag aaaatgaaat
    10981 tcaaaaaggt ggcagatcga taattatgac agaaaaatgg cgtgaagacg aagaatatat
    11041 gtcttatgta caagatcttt tagaaacaga agcagtgaaa aaattagcga attacacaca
    11101 gcatgttcat tccactcgtt tggagcattc catcagtgtt tcttactata gttatctgct
    11161 cgcaaaaaaa tggggtggca atgctaaagc aacagcgcgt gctggacttt tgcatgatct
    11221 gttctactat gattggcgta cgacgaaatt tgacgagggg acacatgcgt atatccatcc
    11281 aagaattgct gtaaagaatg cagaaaaaat caccgatttg tcggatcttg agcgagatat
    11341 cattttgaag cacatgtggg gtgcaacgat cgcaccgcct aagtataaag aaggatatat
    11401 tgtgacattc gtggacaaat actgcgcagt caaagaagcg gctcagccca tgtctgcaag
    11461 tatgcgtaag agatggcaac gttactttgg gaaagaatcc tcgatttaac aggtaaaacc
    11521 aataatagac catattgagg ttgtgacaga agtggtcagc ttcaagaaat aagaaggaat
    11581 ttacgaaaat tacttctcaa atttttgtga agtttcggct tattaccgca gatgcaagct
    11641 tttgaacacc attcattcga aacaagggac tttgacacga cttttgtcac agtccttttt
    11701 tctgtttgca gttgactatc tgttcaagat acggtttact tttatttatc gaatgaatca
    11761 tgtacataga agaataagca ttaaaggggg tgatgataag atgcaaaacg tatcttattt
    11821 aaaagagtcc gggatcttca ggtaacacat actttgcaga agatcacttt tttctacaaa
    11881 gtatgcactt tggttagaag aaaggacttt aaaatcatgg aacaaacaat aaaaccatac
    11941 caatattttt ttataaaaag agaagtagaa cagttattaa atgcgtactc atctgtcaat
    12001 gatccaaaaa cggtacaaac agttcaggca ctagcaactg aaaagattcg agacatatta
    12061 gaacacgaac tgccagaaat cgatacattt ttaacgacag ttttagacgt aaaactgaca
    12121 aaagcgcaag cagaacgagc attggaggag ctgaagcaaa tcgttcagcc gtttatgcag
    12181 cctagcaaac cacaaatcga gaaattattc cgaaaagtga aaaaactcaa acaaccagct
    12241 tggaatgaaa tcgacttgca agaacatacg tacatcggct ggaatgatcc aggtacgcag
    12301 aaaaaattca tgatctatta cgaagatgaa aaattaaaag ggattagtgg tactttatca
    12361 ccaacgataa ataagggaat ctgtgcaatc tgtcagaaaa catcgaatgt ttcgcttttt
    12421 ctttctacaa cgaaagcagg ttctgatggc acgtatacga aaaaggggaa ttatatttgt
    12481 catgatagtg accaatgtaa tcaacagtta acgaaattgg ctcctttcta tgaatttgct
    12541 gaacgtgtaa agaaagaacg ataattttat tgttttttac agaaaaatat gctatctttt
    12601 aatggaagtg aataggtgat ggtgttcgcc tttaaatgct aacgaaagat agctaatgac
    12661 gcctactaaa gtctggggac agcccctttc tttagtaggc gtttttttat tgaggaggaa
    12721 ttttttatgg aaaaacaatt accgagtcca gtacgcattt tagtgtacat gctgaagtgg
    12781 ctggtgatca cagcgattct cggtatattc atggggagct tgtctgcttt tttcttaaat
    12841 agcctgacag ttgtgacaga tatccgacta tcacatcctt ggctccttta tctactacca
    12901 gttagtggtg cgctttttgc ttatctctat gcgtatcacg gcggtctttc tagtcgtgga
    12961 aacaacttag tgatcgatca aggaaatggt ggagaagaaa aaatcccgtt gcgacttatt
    13021 ccacttacac tgtttggaac cataactacc catttatttg ggggatctgt tggccgtgaa
    13081 ggaacagcag tccaaatggg gggagcgtta gcagataata cagcacgttt gttccgattg
    13141 gataaatcag aacgggaaat cttgattatc agtggaatca gtgcgggttt tagttctgta
    13201 tttggcacac ctttagctgg aacattattt ggattagaag tgttggcaat cgggaaagtc
    13261 cgaacagaag cgatcttccc tagcttcttt gctgcactgt ttgcaaattt catgacagaa
    13321 agctatggtg tttctcatct acattaccaa atgggtgtga ttcctgaatg gagtatgttg
    13381 ctgttcttca aagtttttgt tgctgggatt gcttttggtt tgattggttg ggtattcagc
    13441 cgttcgatcg tctttttaaa agcgcgatat gcacaatgga ttcctcatcc tgtttggcga
    13501 aatacgatag gtggagctgt agttgtggca gcagcattga ttttgcaaac acaacgttat
    13561 ctcggattga gtcttccgtt acttcaagaa tcatttaatg gacaggcaca tgtctatgat
    13621 tttattggga agttattctt cacagttttg tctttaggcg cgggatatca agggggagaa
    13681 gtgacaccat tgtttgaaat tggtgcaacg cttggtgcag cactagcgcc tttgctccat
    13741 gtttctgttc cttttcttgc aggattgggc tttatcggag tattttctgg tgcgacaaat
    13801 acaccgattg cctgctttgt gatggggatc gaattgttcg gttcacaagc ggcagtatac
    13861 ttctttatga tctgcttgat cagctttatg tgttcaggca acagcggtat ttattctgct
    13921 cagcaagtag cggtcaaaaa agggatactc tttttgccaa tagttaagag aatgaaagaa
    13981 aaaaacaagg tgtgacatat gttttgtcac accttgtttt tttagaaaag taaagtaatc
    14041 agcgataaga atgcggagcc tgcgaaaagc aatccgcaaa tgatcatatg tctttttttg
    14101 ttcatcatca agatggcgat gtattctctt aaaaacgcat taggcaagta gtagaaagct
    14161 gtttttgcac caataccatt tgctttcaat ttggcttgcc acgcataaag accggctcta
    14221 aaaatgtggt aattgttgga actaaagatc gcgcggtatg gccctttcat ttgctgatcc
    14281 atgatttcct ttgaatagag catgttctcc aaagtggttg tcgaattggt ttcgacaaga
    14341 atgtctcttt cagggattcc ttgttccatg gcatattgtt tcatcgcaat ggcctctggt
    14401 actttttcgt ctgctccttg cccgccagac atcaataaga tgggcggatt taatgtagca
    14461 cggctttgag cccgataaaa agcaattgcc ttattgattc gcttggctag aagaggagaa
    14521 actgtttctc cattgatcaa cccagcgcct aacacaacga tgaagtcttg attgtatcga
    14581 ggccggttga actgatacaa gaatgagaca gtcaaaaagt tgtagaatac gataaataga
    14641 tagatcaaga tcaggggaac gcagaaaaac aaagcattta tccattgcgg caaatatttt
    14701 aacaagaaaa agtcaaaaac aagaaacagt gtaagaaaaa tggctagtat caacgttaaa
    14761 agattagcta atgaatgcga ttctctacgt aaaactgtga ccccgttcca aaaaagaaaa
    14821 acaatcaatc cataaatacc aaataaaagg acaagtagca atggaatcgt gatcaaggcg
    14881 atcagtccgc caaggaaaat attttgcgtt tcatacagca agacaaacaa gtagatgccg
    14941 aaactgatga ggaaaacatt gaataaaaga ccattcagca gccttcgttt ttctgtgaaa
    15001 taagaaaaag caaagatact aaagaacaac aatggaatca aaaaataaaa ggtcgaataa
    15061 aaaatttcca acacgacaaa ataagttaag attaccgtaa atgtccacgt actgccgtaa
    15121 aagaacaagg aaggatcctt cttctgtttt cgccaataaa cgatagagaa taccccaata
    15181 agatagagaa ctaaaaagat cagatctctg gttagattca taaattcaac tcctttttct
    15241 tatacagtat aacgaaaata gatgaaaaaa gctgttattt aaactagaaa aagcaaaaaa
    15301 acatctttcc gatggaaaga
//
LOCUS       DACDRK010000061        15205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDRK010000061 DACDRK010000000
VERSION     DACDRK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.061"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1251)
                     /locus_tag="IWJ77_002026"
     CDS             complement(<1..1251)
                     /locus_tag="IWJ77_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAP9453614.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQ"
     gene            1524..3995
                     /locus_tag="IWJ77_002027"
     CDS             1524..3995
                     /locus_tag="IWJ77_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9453615.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4098..5834)
                     /locus_tag="IWJ77_002028"
     CDS             complement(4098..5834)
                     /locus_tag="IWJ77_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9453616.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5831..6535)
                     /locus_tag="IWJ77_002029"
     CDS             complement(5831..6535)
                     /locus_tag="IWJ77_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9453617.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6666..7169
                     /locus_tag="IWJ77_002030"
     CDS             6666..7169
                     /locus_tag="IWJ77_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9453618.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7129..7467
                     /locus_tag="IWJ77_002031"
     CDS             7129..7467
                     /locus_tag="IWJ77_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9453619.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7488..8906
                     /gene="ffh"
                     /locus_tag="IWJ77_002032"
     CDS             7488..8906
                     /gene="ffh"
                     /locus_tag="IWJ77_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9453620.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9018..9596
                     /locus_tag="IWJ77_002033"
     CDS             9018..9596
                     /locus_tag="IWJ77_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9453621.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            9600..10121
                     /locus_tag="IWJ77_002034"
     CDS             9600..10121
                     /locus_tag="IWJ77_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9453622.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10200..10754)
                     /locus_tag="IWJ77_002035"
     CDS             complement(10200..10754)
                     /locus_tag="IWJ77_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9453623.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11007..11282
                     /gene="rpsP"
                     /locus_tag="IWJ77_002036"
     CDS             11007..11282
                     /gene="rpsP"
                     /locus_tag="IWJ77_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9453624.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11294..11545
                     /locus_tag="IWJ77_002037"
     CDS             11294..11545
                     /locus_tag="IWJ77_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9453625.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11670..12461
                     /locus_tag="IWJ77_002038"
     CDS             11670..12461
                     /locus_tag="IWJ77_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453626.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12631..13152
                     /gene="rimM"
                     /locus_tag="IWJ77_002039"
     CDS             12631..13152
                     /gene="rimM"
                     /locus_tag="IWJ77_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9453627.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13142..13903
                     /gene="trmD"
                     /locus_tag="IWJ77_002040"
     CDS             13142..13903
                     /gene="trmD"
                     /locus_tag="IWJ77_002040"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9453628.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14039..14386
                     /gene="rplS"
                     /locus_tag="IWJ77_002041"
     CDS             14039..14386
                     /gene="rplS"
                     /locus_tag="IWJ77_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9453629.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(14518..>15205)
                     /locus_tag="IWJ77_002042"
     CDS             complement(14518..>15205)
                     /locus_tag="IWJ77_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9453630.1"
                     /translation="AMKKGYVEKNPINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNF
                     TTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITNNNASVVINGTMMYRERSIADM
                     KKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKGTPFQLTAINTYLRN
                     HKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENSDITEKIYLHVTKGVK
                     EKLKEDIEKL"
BASE COUNT         4930 a         2781 c         3105 g         4389 t
ORIGIN      
        1 ttgtaaatag atccgtgccc gaaagttata aaagttgcga taaccaaacg caattcgttt
       61 aatcagcttg atcttgttat tcagcccctc agtgacgccg tttgagtggg acaccttaaa
      121 ggcatttgaa atacccgatt ggtagcgatg gaagatcgtg aatttctttt taaacgactg
      181 aggtaacgta gatgaaattg ttttaagggt ttcttgaaag ccttccgtac ctcgtttaac
      241 gaaataatag cgtaataatt gacaaaaatg atacgcagtt cgcaattctt cgttgtagct
      301 taacagctca tcaacgattt ctgtttgact aattggtcgt ttaaacagtc gatgataatg
      361 ccgtttgagt ggctccaatg tcgtggaatc tttgagtaag agtttccaat atcgcttgag
      421 ccgacggtac ttcttctgat tttcagagtg tgaggttcga aaacgattca tgatctggac
      481 acgtaattga ttgaagctgc gattgatgtg ttggacaata tgaaagcggt cagttacgat
      541 ctccgcattt ggaaagactg ttttaagcaa ctgacaatag ctggcattca tgtccatcac
      601 taggtacttg acctttaaac gggcctttct aggaaagcgc aggaagtatt gagttagttt
      661 gtaaagtctg cgatcttcaa ggatcgtaaa tagctgattg gttgtcccat caacacaaat
      721 aaaactcatg gaaccagaaa cagaattcat tgatttgaac tcatccatac acaacaccgt
      781 cggaaggaag ttcatacgtg gctggtaatg cttcgttgct tcacgcaaga tccgtgtcac
      841 cgtcccatcc gagacgaagt acttcttagc gattagttta cgtgattgat tttcgtacag
      901 ttccatgaga atcacctgct tcagttcctt tgttagttga tgatgttcat caactaagtc
      961 cgttttcgca gaaaaggtcc ttccacaatc atggcagaga aaacgggtac gttttaattc
     1021 taaaatagtt aacttttctt tgactttcaa catctgagtg cgtgtcgtat aggaaccgtt
     1081 gcggataata cgaaccgatc gacagtgttc acaataggaa gaagagtcat ccaattttcc
     1141 tttaataaac tgcgcttgaa cgcctttata ttctttttca atcagccatt cttcatcaaa
     1201 aaataaatgt ttatctgtta atccgagtaa ttttctagta tgattatcca ttgagagact
     1261 cctttggtag gatgacgttt tggtcgacta aattttacca aaagtgagtc tctttttgag
     1321 ttgaaacgaa gaaaaagtgt tagtagaaaa tcgccttcga ttttccacca acacaaaata
     1381 ttatagaacc cttttttttt gctatatgac aggaatgaaa ggtgtttgca agcttaatgt
     1441 gaggaacact ttccatatct gttctatact ggataggaga agtttggaaa tatccagaaa
     1501 acgaaatcaa tcgaggtagc cacatgaaga ataaatggat aaatcgaagt aaaaaaaaga
     1561 aagtaacaca aagatcatca gataaagtca tccgtctttt tccatcgtat aaagaaggat
     1621 taacggcaga tcaagtcaag gaacgaatag aaaaaggtgc agcaaataat tcagtcgatc
     1681 ccacattcaa aacaaatcag caaatcgtat tggaaaacat atttacgtat ttcaatttga
     1741 tctttcttat cttagccatt ttgctttgtt tagtagaatc ctataagaac ttgacttttt
     1801 taccagttat catagccaat acaggaatcg ccatttttca ggaaattcga tccaaaaaga
     1861 tcttggacga actaaatgtt ttaaatacag caaaagtcaa tgtcatacga gacgggatag
     1921 aacaaacaat agatatagaa gaccttgtaa tagatgacat tgttatttta gagactggat
     1981 atcaaattcc agcagatgca gaggtcgtgg aagggaaact gcaagttaat gaggctttat
     2041 tgacaggcga agcagatgaa ataaccaaag aaataggtga tccattaatg tccggcagtt
     2101 tcattgtttc cggaaaagct tacgcccgat tagacaaagt tggagcagat tcgtatattt
     2161 cccagctgac agtcaaagcc aagacaatgg gagaaggaga acagtcggag atgattgctt
     2221 ctcttaatca attgatcaaa tggattggga tcatcattat tcctatcggt atcctgcttt
     2281 tttctcaaag ttactttttt aatggaaaca cgatcaaaga aagtgtcgtt gcaatggaag
     2341 cagcattgat cggtatgata cctgaaggac tgtacttgct gacgacgatt gcattagcat
     2401 taagtgcgac taaactagca aagcaaagag ttcttttaca caatatgaaa agcattgaga
     2461 cattggcaag ggtaaatgtc ctttgtgtag ataaaacagg aacgatcaca gaaaataaaa
     2521 tgtctgttca aaaaatgatc gtctctaaaa aacaagaaat aatcagtcag tcagatcaat
     2581 tggaagcatg cattgctgac tatgcaaaag cgatggcagg agacaatgca acaatggaag
     2641 ctgttaaagc atattttaca aaaaccactg atcaatccta tcaccatgtt attccttttt
     2701 cttctgtcca aaaattcagc agtgtagcgt tggcagacaa ggtgtatgtt ttaggagcgc
     2761 ccgaaatggt tttgcgcgat caattgccgg aatatgcgga agagtttgtt tctttcgctg
     2821 aaaaaggcta tcgcgtgttg gtctttggtg tgtacgatgg gattcttgaa gaaccagtat
     2881 tgacagaagc agtcacacca ttaggctata ttctgattgc taatcctatc agaaaagaag
     2941 caagagccac atttgattac ttcaaaaaac agcaagtagc catcaaagtg atttctggag
     3001 ataatccgct caccgtatcc aatgtagctg tccaagcagg gatttcacat gctgatcagt
     3061 acgttgatgc atctaaatta tcagaagaag aatatgaagc agctgtcgaa aaatatacag
     3121 tttttggacg cgtcaagcca gaacaaaaga aaatcttcgt tcaattgata aagcgtaaca
     3181 atacagtagc tatgacaggt gatggcgtga atgatattct agccatgaaa gaagcagact
     3241 gcagtatagc gatggcttcc ggaaatgaag ccgccatgca ggcctcacaa gtggtcttac
     3301 ttgattctga cttttcacgc atgccagaag ttgttgcaga aggacggcgc gttgtgaaca
     3361 atattgagcg atcagccagc ttgtttttag tgaagaatat tttctccttt ttgctctcac
     3421 tgttttcagt gatctttgct ctgacctatc ctttagaacc atcacaaatc acattgatta
     3481 gtttgtttac tatcggactt ccatctttct tgcttgctct agaagaaaac aaaaagcgaa
     3541 tcagagggaa attcatcatg aatgtgatgg aaaaagctgt gccaggtgga ctaacagata
     3601 tgatcgtagt cggtgcgttg gtgatctgcg gggtgacttt gaatctaaat aaaacagatg
     3661 tttctacagc atctaccatg cttttgattg cagtagggtt cctcgttctc tataaaatct
     3721 gttcaccgct gaataaattc agaagtcaga tcattctttt ctgtgctagc ggtaactttt
     3781 tttcggtcat ctttttgcat aagctgtttt caatcaccgg tatctcagct gtatcacttt
     3841 tgctgttgag cattctattt ttctcggcag attcgatttt taggcatttg accacattgg
     3901 tagaatattt gtttgaacgt aaaaatgaaa aagagcaaaa gccgtttagt cgcgtagcta
     3961 aaaaatttct atcgatattt aaacgagaaa attgacgaaa agtatctcaa aaaagagtaa
     4021 aaaagaacaa ccactcgtgt atctttcgaa gcaggatgag tcatccaact ttaaaacgca
     4081 cgagcagttg ctctttttta cattttcgga atggtaacgg tgaaagtcga acctgttcca
     4141 agatgactgt ctacagtaat cgtaccatta aggagttgca cgtagtcttt tacaatcgct
     4201 aaccctaatc cggtcccgcc tgaatgtcgg cttcgcgcct tgtctacgcg gtaaaaacgc
     4261 tcaaagatcc gttcctgatc ctcaatgtcg atacctatac caaagtcttg gacagaaaac
     4321 gatagatcat ctgttgcctg atagcggata ataatctcgc tgtcgctttt tgaatattgg
     4381 acagcatttt caattagatt tttgataatt ggataaagga tatctgtttt agtagtaaat
     4441 ggttcgttct ttccaatcaa tcgaatcgtc aaccgtttct tttcgatgat tggctgataa
     4501 ttttgaacga tttgctggaa ataaggttct gtataaattg tctggatctc gtaagaattc
     4561 tcgccatctt ttgacagctg gatgatttcc cgaatcagtt tatctaaccg gatcgcatct
     4621 ttttgcatga tctcaagaaa agaagtcaga gtttgcgggt catcttttgc accatccagt
     4681 aaagtctcgg taaaaccaat cagtgaagtc acaggagttt ttaattcgtg agagacgttg
     4741 ccaacgaaat ccttttgtag cttttctaaa cgtcgcactt tcgttaagtc atacgccacc
     4801 cctagaatct gtccggtacc ttctgaatta gtaaagtagc gcaaactcaa gtctaatgtc
     4861 tgccgacctt ctgtaagggt gatctcctga tggatcagcg gatgctcagg agtcacttga
     4921 tgaatcagct gaatgagttt tggctcttga atgacttctg tgtaccgctg ttcaggttga
     4981 tagcttacca cgtttaaatg tagctgcatc tttggattaa gcagtagtaa acgagaatca
     5041 gcatcatcaa tcaggaatac gccaatcatc agttcgttca gtaacgtata taattgctct
     5101 tctgtggtag tacacgcccg atacatctta ctcatttgtt cgctcaacgc attaatcgtc
     5161 tgatacaatt cttcccattg atcgggtgtt tgcatgatta cttccgcctg attaggattt
     5221 tgcaccattt tttttagtac gggcagcacg gttttcaatg gctggttttt ttgatagatc
     5281 agataataga tcattcctgc aagaacaagg aagaaagaca gaaagaagaa aaagacccaa
     5341 cggcgaaaac tctcagttct cggtaaaaaa ccgcttgtcg gttctgcgat acgtaaaatg
     5401 gcttctaact ggccactttt tttgactggt aatgcgacat ataaaagatc ctcattcaaa
     5461 gtagtacttt tgcgcaaagc gctacctagg ttcccaccat tcaatacggc tttgatttcg
     5521 ggacgggagt ttcgctgttc attcaatgtt tcattgtttg tgtcaaataa gatctttcca
     5581 gtagcatcca ttaaagtaat ccgttcgttc gactgatgga cgaattcttc caaagcagtt
     5641 ttgttttgcg ctgcttggag gttttcgaca tctaattgcc ggatcagcag ttgtgctttt
     5701 tttgtcaggt agctttcttg ctgttccacg atctgctgtt gaaaataatg ggaaatcaat
     5761 tgccagccac caaaaaacaa actaactaaa agaataagaa agaagagacg ttcataaatt
     5821 tttttccatt tcatcgtttg ggttcctgaa acttatagcc aaatcctcta atagtcacca
     5881 agtactttgg gtgttttgga tctcgctcga ttttttctcg caaatgacta acgtgtacat
     5941 ctacgattcg gctttgtcct gcgaagtcaa aattccaaat gcggtctagc aatgtgtctc
     6001 ggtcgatcac acggtctttt cgtttcatga aataaacaag taattcaaat tctttcggtg
     6061 ttagttcaat cagatggtcg tctacggtca cttgataatt ggtcaaatca gcagtgattt
     6121 gtccaatcga tagatattca ggctcagctt catcttttga ttgcttacga ggttcaattc
     6181 gtctgaatat tgctttcata cgggctaata cttcgcgagg actaaatggt ttggtcaaat
     6241 agtcgtcagc acctatttct aggccaatga tccgatccac ttggtcatct ttagccgtca
     6301 gcatcaaaat cggtgtttca tttttttctt gacgcagccg ctgagtgatc tccatgccat
     6361 ccatcgttgg aagcatcaca tctaaaatga tgaaatcaaa tgattgttct aatgctaatt
     6421 ctaatccttc gcctccatct gtggcactgg ttaccttgta gccttctttt tccaaattga
     6481 acgtcaagag cgttacaata gaaggttcat catcaacaac tagtattttt ttcattcaaa
     6541 tctcctttaa ataggtttca ttctgtttat attttagcat agaatcagta caaagggcta
     6601 ttttacaaaa gaaataaatg gtataatagg aaggcacttt gctgaataga aaggaagagc
     6661 agtcaatgat tggaatcagt gcatgtttag gcggtctggc ctgtcgctat gatggacaga
     6721 aaaaagaagt accagaattg atggaactag ttgaaactgg agaagctgtc atggtctgtc
     6781 cagaagtgat cggcggatta ccaattccta gagatcccgc tgaaatcatt ggtggaaatg
     6841 gattcgatgt gtggaaagat caagcaaaag tatggacgac aagcggcgaa gatgtaacgg
     6901 aagcgtacaa aaatggcgcg atcaaagcct atcaaaaatt gcaggaaaaa cacattgatc
     6961 tagtcatcat gaaggaaaaa agcccttcat gcggaaccca ttcgatttat gacggaagtt
     7021 tttccggaat aaaaaaaaat ggtgccggag tggctactgc ttattttatt cagcaaaaaa
     7081 tgactgtgct ctctgatgaa gaatggctga aaactagagg tgagcagaat ggaaatcgaa
     7141 aagaccaatc gaatgaacgc gctctttgaa ttttattcga ccttattgac agaaaaacaa
     7201 atgaactaca tggaattata ttatgcagat gatttttcat taggtgagat cgcggaagaa
     7261 tacgaagtaa gccgacaagc agtatatgac aatatcaagc ggacaagcaa gatcttggag
     7321 gattatgaaa aaaaactcca tcttttttct aattatattg ttcgagaaca ggtattggaa
     7381 aatatgactg catatattac agataagtac ccggaagata aaaaactact tgaatatgta
     7441 cagcaaattc aagcaatcga agaataaata aaaggaatga agaaaatatg gcatttgaaa
     7501 gcttaactga acgtctccag caggcgatgg gaaaaataag aaaaaaagga aaagtatcag
     7561 aagctgacgt aaaagaaatg atgcgggaaa tccgccttgc cttactagaa gccgatgtta
     7621 acttgcaagt agtcaaagat tttactaagc gcgtacgcga acgtgcggta ggggcagaag
     7681 tgctggaaag tttatcacct gcacaacaaa tcgtaaaaat cgttgatgaa gaattgacga
     7741 tcacacttgg ttctgaaaca gcagagttaa ataaatcacc aaaaatacca actgttatca
     7801 tgatggctgg tcttcaaggg gcaggtaaaa cgacctttac tggtaaatta gccaattatt
     7861 taaagaaaaa tgaaaatgct cgtccgctcc taatcgcagg ggacgtatat cgaccagctg
     7921 cgatcgacca attgaaagta ttaggtcaac agctagacgt tcctgtattt gatatgggaa
     7981 ctgaagtcag cccggtagag atcgtccgtc aagggatgga gttagcaaaa gaaaagaaaa
     8041 acgactatgt attgatcgat acggcaggac gtctgcatat cgatgaaaca ttgatggatg
     8101 agttgaaaca aatcaaagaa ttgacgcaac caaatgagat tttgcttgtt gtcgatgcca
     8161 tgactggtca agatgccgtc aacgtagctg atagcttcaa ccagcaatta ggtattacag
     8221 gggtcgtcat cactaaactt gatggggata ctcgaggcgg ggcagcacta tcgatccgct
     8281 cagtgacagg cgcaccaatc aaattcatcg gttctggtga aaaattgact gacttagaag
     8341 tattccaccc agatcgtatg gctagccgta tccttggtat gggggatatg ctgacattga
     8401 ttgaaaaagc gcagcaagac tacgacgaga aaaaagcgga agaacttgca cagaaaatgc
     8461 gggaaaattc attcgatttc aatgacttta ttgaacagct tgatcaagtg atgggtatgg
     8521 gcccactgga agatctaatc aaaatgatcc caggaatgaa tcaagtccct ggaatcgaaa
     8581 acgtcaaggt agatccaaaa gatgttgaac gcaaaaaagc gatggtttat tctatgacgc
     8641 cagcagaaag agcaaatcct gatctgttga acccaagccg tcgtcgccga attgctgccg
     8701 gttctggaaa cagtgttgtt gaagtgaatc gaatgatcaa acaattcaag gaatcaagaa
     8761 aaatgatgca gcaaatgtcc aaaggggata tgaacatccc aggtatggac caaatgtttg
     8821 gttcaggcgt caaaggaaaa ctaggcaaaa tggcgatgaa tcgaatgatc aagaaaaata
     8881 agaagaaaaa gaaaaaacgg aaataagatg aaaaaaacag cttcggtaca gactgaaaat
     8941 gtctactgcc ggagcttttc tatcagcaat ttttattctt tactaataca attactctaa
     9001 acgaagaggt ggcacagatg agatgtccgt ggagtgaaca aacagaaagt atgcggcaat
     9061 atcatgatac tgtctggggt gttcctgaat atgatgacca aaagctgttc cgaaaattga
     9121 tgcttgatat taatcaagcc ggattgagct ggcagacgat tttaaataaa agtgaagcat
     9181 tcgatgaagc atatgatcga ttcgatatca aaaaagtcgc aaattatgga gaaacaaaaa
     9241 tcagtgaact aatggaaaac aaaggaatta ttcgtaaccg aagaaaaatc gaagcagccg
     9301 tccaaaacgc caaagcagtc ttgaaaattc aagaagaaat cggcagtttt tcagagtatc
     9361 tttggtcgtt ttctaatgga aaaatcgttg attcttctta tcattcacaa gaagaagtcc
     9421 caacaacaag tcctctttca gatgaattag caaaagatct aaagaaaaga ggatttaaat
     9481 ttatcggaag cacaacaatc tatgcttttt tagaagctgt cgggatcatc aatgatcacc
     9541 ttgacagttg tttccgcaaa gaagaggtcg tacgtatcgg cagaaagggc gaataaagca
     9601 tgcgaaaaat catattttat ggtgctataa gtttagatgg ctatttggca acgaaaacgg
     9661 atgatcttca atggcttttt gacactccaa caggtaagca aactacttat gaagcttttt
     9721 atgaaacaat cgatacgacc atcatgggca gaaaaactta tcaggaagca aaaaaatatt
     9781 tggagaccga agcaatttat ccagagaaaa agaatttcgt tttttccacc cacgcacaat
     9841 tacaattgcc agatgctgag gtcgtaaacg aagatcctgt tgcttttcta aaaaatctac
     9901 aaatgacaga aggaaaagat atctgggttg tgggtggcgg tcgattgatc aaaccgctat
     9961 tggaacatca aatgattgat gaatggtata tccagattac ccctgtatta ttaggggacg
    10021 ggattccttt attccaaaaa ggaacatatg aagcgcgttt tgaaatgcta gacacgacac
    10081 gattcggtga atttatcgaa ttgcattatg tacgaaagta gaagtaaata aaataaaaaa
    10141 ggcaactata tgcagaaatg tcctgtacat agttgccttt tttattaccg ttatgctttt
    10201 tactctgtta atttgttaga aaaatctaga tacgcatcta aaatcgtttg gaagaaactt
    10261 aatgttcctt caacgatatt accattttca tcaattagat tcgcaacgtt tccgatatat
    10321 gcttccggtt gctgcaaggt tggcacattc aaaaagacaa gtgattggcg tagatggtgg
    10381 tttgcgccaa agccgccgat cccacctggt gatgcagtaa cgactaaacc tggtttatga
    10441 tcccaaacac tgtgtccata aggtcttgag cctacatcta aagcattttt caatactgct
    10501 ggcacagaac ggttgtattc aggagtaacg aaaacgacac cgtctagtcc cctcacttcc
    10561 tcacgaaaac gagtccattc agcaggagcc tgttctggag tttcgagatc ctcattgtaa
    10621 aatggcagat catcgatttt tacaaataca ctttcgtacc cttcaggaag caatttcgca
    10681 aaagcttctg ctactttttt gttgtaagaa tcttttctta aactaccaat aaaaaatcca
    10741 attttttttg tcatacgatt tttcctcctt caaactatct ataataatct tacaaaagat
    10801 aagtaactgg agcaaataaa aacacttact gacaaaagct acatagaaga tagattactt
    10861 atttttaaca aaaaagaagt ctaaaatctt ctattttcta aaaaaaatcg aatttaatca
    10921 agctgtaaag aaaaaaagct ttacagggta aaaaaaaact gttacactac atcttgtgaa
    10981 taaaacaatg gaggtggaaa taaataatgg cagttaaaat ccgtttaaaa cgtatgggtt
    11041 ctaaaaagag tccgttttac cgtatcgtcg tagctgattc acgttctcct cgtgatggac
    11101 gtttcatcga aactgtaggt acttacaatc ctttgaaaga ccctgcagaa gtagttttaa
    11161 aagaagactt agttcttgac tggttatcaa aaggtgcaca accttctgac actgtacgta
    11221 acatcctttc aaaagaaggc gttatgcaaa aacaccacga agctaaattc tcaaagaaat
    11281 aaggtgaccg gatatgaaag atttaagcga cttagtctta acaatcgttc gtccgctagt
    11341 cacttatcct gatcaagtca agttagacgt cgtagagtcg aaagactttt acgagtacaa
    11401 tctaacagtg gctcctgaag atattggccg tatcattgga aaacaagggc gtgttgctaa
    11461 agcaatccgc acaatcgttt acggtgttcg gatcaatgca ccgaaaaaag tgcgtttgaa
    11521 tatcatcgat gataaggaaa agtaaataaa cgtgctgtac tcgaattttc gagtgcagct
    11581 tttttgtttt ttttattaat tgaagaaatg attatttggc aaaacaagca agaaagcgta
    11641 tccttatagg aggagggagt gaacagccaa tgagaaagaa aatgttattt attccatttg
    11701 ctctattgtt aagcgcttgt agttcggtta ctcaagaatc agacaataca catctatcaa
    11761 gcagtatatc tgaaactgtt accagtccag aaaaaagtag taccaaaacg gcttccacca
    11821 ctgaacaaac aatggctacc tcaaataatg agaaaaagac tgctttagac cagttgaaag
    11881 aacaacagcc aaatgttccc atgcctctag atgtaccagt ctcttcaggc tatctcaata
    11941 tcgcagcgac acatacaaag caaggttatt ctattttata ttacagaaca gatcgtcccc
    12001 tcgggttgaa tgctgacgaa ttgaatcaag aaacgcctat tgcaacctat ctatatcaat
    12061 atgggtttgc ttctagtcaa gaaacaatcc aagttttgca gccatttgag atcgacacaa
    12121 atggtcagca agtggactta ggttctcgaa tcacagggta tcagcaaggt gcagcaggtt
    12181 caagttttct tgaatggcaa gaaggaaact ggcgtattcg aatccgtggg aataatatag
    12241 aaggccaaga tcccttacta ttagctaaag aaatcgtagc ttatttggaa gaaaacagtc
    12301 taccagcacc tgaacaattt ggtaaaatta ctgttgatat gggtgatacg acgaatcgag
    12361 ccgtagaagt aagctggcaa gaaccgaaaa atgcctatac gatcactcat caagatccta
    12421 tgtctgcgtt gaagatggca gtttctatga agaggttgtg aaatcagcgc gctgatctga
    12481 gcccatcatt taactccaag tcacaggaag taatcaaaaa tagcacgccg catttttgca
    12541 taattttggc ttaatacctt aaaagaaaac tagaattgcc tcttcacaca tgctaaaata
    12601 aaacagacaa aaaagaaatg gggatataag ttgactgaat acttgaatgt aggaaaaatc
    12661 gtaaacacac aaggtatcaa aggcgaagtc cgtgttatct caacaacgga tttcccagaa
    12721 gaacgttaca aaaaaggaac ggtgctaaca ctattccaag acggaaaagc acctgtggaa
    12781 ttaacagtca aaagtcatcg caaacataaa aattttgact tattgagttt tgaaggacat
    12841 ccttctatca atgatgtgga aaaatatcgg gatggcatat taaaagtgtc aaaagacaac
    12901 tctgtagatc tagctgaaaa tgaattctat taccatgaga tcatcggtct gcatgtatta
    12961 gaagatggaa ctgagttagg taaagtaaaa gaaatcttgt caccaggtgc caatgatgtt
    13021 tgggtcgttc aacgtccgaa aaaagctgat atattgcttc catatatcgc ttctgtcgtc
    13081 aaagaagtcg atttagaagc tggagtggtc cgtgtagaaa ttccagaagg actgattgac
    13141 gatgaagatt gatgtattga ctttatttcc aagaatgttt gaaggtccta tgggggaatc
    13201 catcatcggt aaggctgtag ataaaggatt attagatatc aatatctcta acttccgcga
    13261 ttattcagat aacaaacatc agacagtaga tgattatcca tacggcggtg gagcaggtat
    13321 gctgctcaaa gtccagccca tctatgataa catccaagcc attgaaaaag ctgcaccgga
    13381 tgtcaaaaaa agagtaattc ttcttgaccc agctggtaaa cgattcgatc agaaaatggc
    13441 agaagaattt tcacaagaag aacacttagt attcatttgt ggtcattatg agggatatga
    13501 tgaacgtatc cgcacattag tgacagatga agtttcctta ggagattatg tccttactgg
    13561 gggagaatta ggtgcgatgg tcatgatcga tgcaacagtt cggcttttgc cagatgtgtt
    13621 agggaatcag acttcagctc aaacggattc ttattcaacc ggtttattgg aacatcccca
    13681 atatactcgc ccggcagaat ataaagggat gaaagtacca gaagtattga caaatggaaa
    13741 tcataaattg attgaagaat ggcaattaaa agaatcacta agaaggacct atcaacgtcg
    13801 gcctgatctt ttggaaacac ttgaatggac acctcaaatg acgaaatttt tagaagaaat
    13861 caagaaagaa gaacaagaaa aaacagcaga tgaagccaag tgactttttc taaaatcttc
    13921 tttacagtag gttgataata tgttagtatt ttatggtgag tgcaaaagca cttaactatt
    13981 acgatattcc gctgtgagaa aagctcataa gaatatttgg aataaggaga agaaaaccat
    14041 gaatccatta atcgaagaat taacaaaaga acaattacgt tctgacatcc cagcgttccg
    14101 ccctggtgac actgtgcgtg ttcacgcaaa agttgttgaa ggtactcgtg aacgtatcca
    14161 gttatttgaa ggtgttgtta tcaaacgccg cggtgctgga atcagcgaaa cttatacagt
    14221 acgtaaagtt tctaacggag tgggcgttga acgtacattc ccattgcata caccacgtgt
    14281 tgcaaaaatc gaagtagttc gctacggtaa agtacgtcgt gcaaaattgt actacctacg
    14341 tgcattacac ggaaaagctg ctcgtatcaa agaaatccgt cgttaatcat cgttatcaga
    14401 ctgagaaaga acccttggta ttcaagggtt ctttttttgt tctctgtttt tttatattaa
    14461 cgatttctat ttgggacatt tttgattaat aaaaaggggc aaaaaagggg caacaagtta
    14521 cagtttttct atatcttctt tcaatttttc ttttactccc tttgtcacat gcaaataaat
    14581 cttttcagtg atatcactat tttcgtgccc aacgcgatcc tgaatagcat acagaggagt
    14641 tcctagttcc gctaattttg aaatatgggt atgtctaaaa atatgtgaac taagtttttt
    14701 atcaatcttc atgtcagctt tatgatttct caaataggtg ttaattgctg ttagttggaa
    14761 aggagttcct ttcgttgttt gaaatagaaa ttgaccattc ggatttaatt ctagaagctc
    14821 attgtaaata gctatcgctt tttttggtaa atcaatttct cgcattccag cagcagtttt
    14881 tgtagaatca gattttttca tatcagctat tgaacgttct ctatacatca tcgtcccatt
    14941 tataactact gatgcattat tattggtaat gtgtacatcg tctttactta atgcgattgc
    15001 ttctccaggc ctcattccag tcaaatatag ccactggaaa aggagagaat atcttttatt
    15061 gtgtgaagtt gtgaaattta ctagtctttt atattcatca tcttctaaaa acttatcttt
    15121 aatttttatc gtttttgact ctcgtttata atcaataact acttcgttga taggattttt
    15181 ttctacatag ccttttttca tagcg
//
LOCUS       DACDRK010000062        15152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDRK010000062 DACDRK010000000
VERSION     DACDRK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.062"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(79..1230)
                     /locus_tag="IWJ77_002043"
     CDS             complement(79..1230)
                     /locus_tag="IWJ77_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9453631.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1384..1914)
                     /locus_tag="IWJ77_002044"
     CDS             complement(1384..1914)
                     /locus_tag="IWJ77_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453632.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(2013..2363)
                     /locus_tag="IWJ77_002045"
     CDS             complement(2013..2363)
                     /locus_tag="IWJ77_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9453633.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(2520..4049)
                     /gene="ahpF"
                     /locus_tag="IWJ77_002046"
     CDS             complement(2520..4049)
                     /gene="ahpF"
                     /locus_tag="IWJ77_002046"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9453634.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(4059..4622)
                     /gene="ahpC"
                     /locus_tag="IWJ77_002047"
     CDS             complement(4059..4622)
                     /gene="ahpC"
                     /locus_tag="IWJ77_002047"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9453635.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4766..5428)
                     /locus_tag="IWJ77_002048"
     CDS             complement(4766..5428)
                     /locus_tag="IWJ77_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9453636.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            5612..7258
                     /locus_tag="IWJ77_002049"
     CDS             5612..7258
                     /locus_tag="IWJ77_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9453637.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            7249..8331
                     /gene="uxuA"
                     /locus_tag="IWJ77_002050"
     CDS             7249..8331
                     /gene="uxuA"
                     /locus_tag="IWJ77_002050"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9453638.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(8439..9992)
                     /locus_tag="IWJ77_002051"
     CDS             complement(8439..9992)
                     /locus_tag="IWJ77_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9453639.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            10140..11428
                     /locus_tag="IWJ77_002052"
                     /pseudo
     CDS             10140..11428
                     /locus_tag="IWJ77_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(11554..12633)
                     /locus_tag="IWJ77_002053"
     CDS             complement(11554..12633)
                     /locus_tag="IWJ77_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9453640.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(12736..13176)
                     /locus_tag="IWJ77_002054"
     CDS             complement(12736..13176)
                     /locus_tag="IWJ77_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9453641.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(13233..13643)
                     /locus_tag="IWJ77_002055"
     CDS             complement(13233..13643)
                     /locus_tag="IWJ77_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9453642.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(13823..14257)
                     /locus_tag="IWJ77_002056"
     CDS             complement(13823..14257)
                     /locus_tag="IWJ77_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9453643.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(14357..15127)
                     /locus_tag="IWJ77_002057"
     CDS             complement(14357..15127)
                     /locus_tag="IWJ77_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9453644.1"
                     /translation="MFLGPHHIAAGGVSGLGILLEAALGFDRAIVILVLNIVMLVLAL
                     LFLGKKPFFKVLFGSLVFPLIIAIVPETMITSDRLLSVIFGSAIFALGVAILYKNNSS
                     SGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFIFGIEEFLYAILSIAITSIVMT
                     YIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLLEAKGGYNRKSKEVLLIVVTD
                     HEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE"
BASE COUNT         5176 a         2853 c         2641 g         4482 t
ORIGIN      
        1 tgtggcgtta tcgtcacagt ccttttttca aataaacggt tttcacgatg tctctttcac
       61 aacttctttt tgatttactc acacttcata aacttttttt gtaaaatatt tgagaacaag
      121 tggagaaata agtgtactta ataaaatcac gataactaaa ggtgaatagt actgtggttc
      181 gatcaattga ctttgctgtc caatttgtaa aatgatcaaa gccatttctc cacgcgaaat
      241 cataccagca ccgaccatca atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa
      301 ataacctcca agcaatttgg tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag
      361 ttgttcacta aagtcagaaa aatcaacttc taatcctaca ctgacaaaaa atactggtat
      421 aaatactgca tatcctaaag cctctacatt gtggtatact tcttctttaa cctttgtctg
      481 actgacagct atcccagcaa aaaaagcacc aatgacagag ctaagcccaa caagatccgc
      541 tacgtaagac attcctaaac aaataactag cgatacgatg ataatagaag aatttgcgta
      601 cactttttct gcaaatgaaa gtaggaaagg cgcaatccat ttgactaata agaaaataaa
      661 aacaaaataa attagctgtt caatcaaaac aagcggtaaa gaagcattgc tagaagcttc
      721 ccccgttaga aaagataggc tgaaacttag aactaaaacg actaaaatgt catctaccac
      781 agaagcaccc aaaattgttg atccttcttt tgtattcact acgtttagtt cttttaatac
      841 ttctactgaa atactgacag aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt
      901 tcccgaaact tggaatgctt tgcctgtcaa ccagccaaaa acaaccggga agatgatccc
      961 aagcaaagca acgaacatac caggtcggaa atattttttc aaaagagata aatcactttc
     1021 cagaccagct aaaaacatta ataagatcac accaatctcc gaaaagtcat ggactagaat
     1081 atcaggtttg acccaattca atccagcttg gcctagcaaa acaccaacaa gtaattgtcc
     1141 aatcactgct gggataccta atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa
     1201 tatcaggcat aaaataccaa taaattccat tctcatcatt cctttgtata aaatatagcc
     1261 cgaacttagc ataacataca ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa
     1321 caatccaaaa gatttttttg gattgttggt ttttatacgg tctattttaa taactaaaaa
     1381 tgattaacgt atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat
     1441 gaaaatcaaa ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa
     1501 caataagacg aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc
     1561 ttttgtattc atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag
     1621 aagaatcggc aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat
     1681 caggaaaaaa agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg
     1741 gtaagcctca aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc
     1801 gataaaatga aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac
     1861 agcagctctt aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga
     1921 aaacctcctt tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa
     1981 atgagcgaag gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt
     2041 ttgccttttg ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta
     2101 tcctcgatgt cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga
     2161 ttggggacag gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat
     2221 tctagttctg tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt
     2281 atacgatctt ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc
     2341 gtaaaaatac gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac
     2401 ttatagtata taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt
     2461 gacaaaattc ttgtcaccat ccttttttcc gctctctgac tttttacaat cttcatagct
     2521 caagtgcgca tcagataatc gaatgctcct aaagctgcag tagctcctga acccattgaa
     2581 acaatgattt gtttgtatgg actatctgta caatcaccag ctgcgaaaac gccggggata
     2641 ttcgttgcgc catttttctc ggtcactatt tcatttcttg gattcatttc gatcgttcca
     2701 tctagccaat ccgtattagg gagtaagccg actagaatga atactcctga aacagctagt
     2761 gtatgcatct ccatcgtacg gcgatcgata taggagagag attctacctg attcgtaccg
     2821 ctgatttctt tcgtcgctgc atgtttgata acagtgacat tcgcaagaga gactagttta
     2881 tcctgtaaga tttgatctgc ttttaattct ggtaagaatt ctagaacata tacatgtttt
     2941 gctaacccag ccaaatcgat tgctgcttca acaccagaat taccaccacc gataacagca
     3001 atctcttttc ctttaaataa agggccgtca cagtgtgggc aataagcaat tcctttattt
     3061 ttaaactctt tttcgcctgg tacattgata gaacgccagt gcgcacctgt cgcgatgatg
     3121 gcggtttttg ctttaagtga tataccgtta gctaagccaa tattaataaa atgttgctct
     3181 ttatttaagg aaacggcctg ttgattcatc ataatgtcga tagggtaact acgcacatga
     3241 ctttctactt gctgcatcaa ttttggacct tctgtgtaag aggtaccaat catattttca
     3301 atccctagtg tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt
     3361 cctttacgtg ctgcatagat agctgcacta ctagctgctg gtccaccgcc aattactaat
     3421 acatcataag gttctttttc aaacaactct tcttcaatca aagggccagt gattttttca
     3481 acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa aaacacagtt
     3541 ggcactgcca tgattttttt atcatcgatt tctttttgga acatcccacc ttcaatcatc
     3601 gtatgagtaa tatttggatt taatacagct aatatattca atgcctgtac aacgtcgggg
     3661 caattatgac aagtcaagct aacataactg tcaaaatgca atgtttgtgt tatttttttg
     3721 attttaagac tcaaagattc actgattttc ggtgcccgtc cactaatttg taacatagcc
     3781 agtaaaaaag aggaaaattc atgaccgagg gggataccag caaaaacgat tccactttct
     3841 ttgccaactg gattgataga aaaactaggt gtgcgcatcg aatctctttt ttcccaagag
     3901 attttaggag acatttctgc tatttccttc gtaaattcag ctagtttttg actattttca
     3961 ctattatcca aactaacaga aaaaacaata ggagattcaa tcagtgctaa atattgttcg
     4021 agattttgtt tagtttcttt gtctaacata tccaccacct aaatctttcc aacaagatca
     4081 aaacttggtt tcaatgtttc gccactttct ttccattttg ccggacaaac ttcacctggg
     4141 tgggtacgta catattgagc agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg
     4201 attccatctg cattgatttc catagcttga acgattccgt ctggatcaat aataaatgtt
     4261 cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga aatctgatga
     4321 gacggatcac cgatcatcac gtattcgatt ttaccgatag catctgatgt atcatgccat
     4381 gctttgtgag taaagtgggt atcggtagag caagaaaaga cttcaacacc taaagatttc
     4441 aatgtactgt attgttcttg taagtcttct aattcagtag gacaaacaaa agtaaaatca
     4501 gctggataga agcaaagaat actccagttc cccataagat ctttttcact gacttctaaa
     4561 aattctcctt gatgataagc atgtgcatta aattcttcga tttttttccc aatcaatgac
     4621 atagtaaaac ctccaataat aattatttat ttataattat tgtaatttag ttggagttgc
     4681 aaagtcaatg gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa
     4741 aaatagaaga ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa
     4801 cagtttcctg ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt
     4861 ctggtttttt atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc
     4921 atggaagccc ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt
     4981 catctaacca gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata
     5041 aattatctgc acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt
     5101 ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca
     5161 tgatttgctc ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa
     5221 ttttggaaac atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt
     5281 gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt
     5341 cagatatttt ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat
     5401 aagcttgtga ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt
     5461 agtataacag aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta
     5521 taaatagtaa aattttggtt gacatagcga tttttattgt ataaaattca cttgaataca
     5581 aaaactaata tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt
     5641 tatccctgaa ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag
     5701 agaaaatgga caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg
     5761 atttcatgca gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat
     5821 tgtttgtgaa acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt
     5881 gattcttgaa gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac
     5941 agctgacagt ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca
     6001 ctttgaaaat cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt
     6061 gaaagataca aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa
     6121 gaagccatca catacaatga gtattcttgc tgcgctactt tataaacgtt ttttatctgg
     6181 agcttggccg attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg
     6241 tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt
     6301 tatccaatac ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat
     6361 caccccgaat ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat
     6421 gtcgattttc caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat
     6481 ccattattta atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg
     6541 ggtattcttg actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg
     6601 ccttaatcct ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat
     6661 agctgaggag atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga
     6721 aggcctccca gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt
     6781 aattactgtg cgcttttcta atccaaatat tcctgatatg cctcagcgaa tcgccacgga
     6841 tacctctcaa aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga
     6901 agttaaagac ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata
     6961 ccttattggc attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac
     7021 agactttcag cataagcttt ccgatattca actgggtacg caagatacat cacatattca
     7081 tgagaaaatg gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc
     7141 agagcttggc agaaaaatag aagaaatgtt catcaagatg gtagcaggac cgaatgccgt
     7201 acgaaaaaca ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact
     7261 tttagatggt acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct
     7321 gcggtcaaag ggattgtagg cacattgatg gatataccag taggagaggt atggcccagg
     7381 gagaggatca aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt
     7441 gaaagcgtca atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc
     7501 gaaaactata agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat
     7561 aattttatgc ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc
     7621 tcatccacgt tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg
     7681 cgcatcgaac attccagcaa tgaatttgaa ttgccgggat gggaacctga gcgcttagct
     7741 catatcaaat ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat
     7801 tttgcctatt ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct
     7861 gtacatccag atgatccgcc atactcgatt tttggattac ctcgaatcgt aaagaatcga
     7921 gaagatttag attggttgtg ccgcatagta gacagccctt ataacggaat cactctttgt
     7981 acagggagta ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgt
     8041 caaagagatc gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat
     8101 ttctatgagg caccgcataa atccgaatac ggttcattag acatgtaccg catactaaaa
     8161 acaatgtatg acaacggatt taacgaatac atccgtcctg atcatggaag aatgatctgg
     8221 aatgagacag gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta
     8281 aatggattat gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa
     8341 ctgtaatttt attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg
     8401 ttgttaataa gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct
     8461 tttttcgatt gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc
     8521 ataatcactt ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg
     8581 atatgagaag gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata
     8641 aaatcatttt gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga
     8701 tctttactga tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt
     8761 ttgtagcgat catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct
     8821 aaaataactg gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg
     8881 gcggtttgga cttctttcaa cacgctatgt tcttgataaa gcatgaaagt aggaaggggc
     8941 gtatttggtt gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat
     9001 gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa atacagtaca aaaggactcc
     9061 accatttctc ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc
     9121 tccaagcttg tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact
     9181 aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagggatca
     9241 ataaaaaagt caatatattc atactctgtg aattcttttt gtacgtcttt cacagaagtt
     9301 gcaggaaaat tctctttaca tatttttaat agaaaatata atttatctat cataccgcta
     9361 tttagtcggt caagtacttc ttttttaaaa aaagtattca tcataaaagc cactacttgc
     9421 ggatcttcat cataaaattc tgtagcagta cttgaaaaga tgttccaata aatatctaat
     9481 gctgcatatt gaatttgtac ctcttttcca gaaaaagact ttctgccatt attagaaaag
     9541 gagatagaca aaccgtacaa tgctaaaaat gcattaatct ttttcatttt cgtatacaaa
     9601 taagaggcac tatagtttgt ttgctgactt aaactgataa tcgaatttgt ctgacagctg
     9661 aacaaagaag tcaagatctg ataactgact gattcgttgc agtatcttcc aaatagacga
     9721 taatagcagt aagccggatc aattccttca aaattcaact ttaaaaaagg tttttgctta
     9781 tcaatatgca gttctgttag attgagacgc tcaaagtctt gttctaattc tttgatgtat
     9841 agtaaaaaat ttgattcaga catatggaaa tgatctgcta aatattgtgt agtaataaag
     9901 aaacgatgtt cagataggaa aagaaaaata tctaattttg tattgctttt tttcttcaag
     9961 agaaacgttt taatcgcttc ttgagtttgc atgaaagagg ctccttttta cttaaattgt
    10021 taaaacttga aaatattact gtttttttca taaatttaga caaaaaaaca gtaaacatat
    10081 aggtagaatc tctactatct tatttcttgc atacaaaaaa atcaaggagt gagagagtta
    10141 tgagatattc aaccatctta atatcaacat ttgcagcctt aagcttatcg cttactataa
    10201 atcagacagt ccacacagag gagatagaaa cagccaaatg gacagaaacc aagtgggcca
    10261 acgtcaatcg aattaccttt caggatgaat tttcatcctt caattcagga gtcgcaaaat
    10321 taagaaaagg acaaacagcg accatcaaag caaaaattga ctattcagga gacttagcac
    10381 ctatactaca atcagctgtt acatttgaga atgttgatcc cgctttgtcg attggctatg
    10441 acgagaacac tttgactatc caacacaagc aagcgatatt taccttcacc gttacattaa
    10501 atcaggactt gttaaaacca gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg
    10561 atcatcatca tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagctatcg
    10621 actcaggagg agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag
    10681 aagtaccgcc tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag
    10741 aacccgatac agaaactcaa ccagagccag acaatgaatc aaaacctgaa ataacccctg
    10801 gagaaaaacc agatgtagac ccagaagaaa aaccagatgt gactccagaa ccagatacgg
    10861 attctggaaa ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc
    10921 cagaaaaacc agaggtagcc ccagaagaaa aaccagatgt aactccagaa ccagatacgg
    10981 attctggaaa ccaaacagta ccggaaacaa atccagacac agataatgaa actgaaaatc
    11041 cagaaaaacc agaggtagat ccagaagaaa aaccagatgt gactccagaa ccagatacgg
    11101 acgcaagaga ccaaggaata ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa
    11161 aaaaagagag taaaaaatca aatctagcaa ttctaaaaat aaatgaagag cagctgaata
    11221 aaaaatcacg aatatttgat agcgcccaat cagctgaaac cttaaaaagt agcaaggata
    11281 caacctttgc tgtcctgaaa cgaaaaataa acagctacca aaatccggag agtcacaaaa
    11341 caaagttata ctgtggagcg gaatcattct attaagtata gcaacaatgc tttctgcaaa
    11401 aagatttaaa caaaacagat ctctctaaca tttgaataat gtctgtgaac gcatttttca
    11461 aataaaaaga aatatccaga aaatgacccc aaaataccag attgaaatct gatatttggg
    11521 gtcgttcata ctttatcttt cttcatattt attttaaggg atatctgttt cttttggaag
    11581 atggttcggg atctttttca ctttcaatac atctaaaaag aaggtaaaga tcggaatgcc
    11641 gacaatcaag ccccaaacgc caaagaaccg ttcgctaaca agtaagacca caaatgtata
    11701 aaaaatcggc agttctgttt tgctggacat caacttcgga ttcagcacat aagactcaat
    11761 tagatggaca atcaaaatcg taatcaggat ataaacaaca tctcggatgc ctccttggga
    11821 ataagctatg aaagatagag gaatacaaga aatgataaca cctgctactg ggatcagact
    11881 taaaatgaaa atcatgatcg ctaaactcag cagctgatga aagccaaata cagccaaggc
    11941 gattgtagtg agcaacgtat ttaaaagagc gacaacaaat tgagcttcta ataccaaacc
    12001 aaatgtattg acaaacttat cggcaaaata gtaaatatct tgaaagaacc aagaaaattc
    12061 actttttaaa aacaatcttg agaatacaat tgtttttttc ttttcaatca taaaaaagaa
    12121 acttaagatg aatgacatag caaacgccac tgccattttt cctatatcct gtaaatagcc
    12181 aagcgcaatc gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt
    12241 atggatatat tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa aattaataac
    12301 ggactggatc atgtcaaatg tttgatgaat aagcatcggg atatactttg tgaatgccag
    12361 atagataagg taaacgatca gtgtataaat catgatagct aaaacacctg tagggattcg
    12421 gacaaatcgc tgaatgattt gaacaagatg aatcatcaaa taagtaaaga taaatgtgag
    12481 caaaatggtt gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa ataataaaac
    12541 agtgagacgg cgtatttttt cattcgccaa aaaacgctga taaacggtca aatcgacaac
    12601 ctccaaataa caaaaaataa acacgttatt cattatatga tggaatatga aaataagaaa
    12661 gagcaaaaag aaaaaccgct aaatgacgtt atcttatgag ttggttttcc tcataaaatc
    12721 gttcatctag cagtttcagc cctttttctg gtcttgatga aaattatcta gcatttttcg
    12781 ggtcagatgt tgcagtgtat ccaattcttc ctgttcaagt ggaagataag acttgatctt
    12841 ttgtggaata ccttctgctt gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt
    12901 tacgattctt tcatcacccg tttcacgctg acgattaata taattttttt ctgctaactt
    12961 ttttagtaag ggagtcagcg taccagaatc cagacacaaa tcatttccta gctctttcac
    13021 tgaagtcttt ttcttttccc ataaaactaa catcacaaga tactgggtat aggtaagatt
    13081 cagcggcttg aggaggggag tgtagtgtct aatgatttct ttagaggcgg catataaagg
    13141 aaaacatact tgattaacta aatatagatc ttccattttt ccacctccgc tgttttacta
    13201 tagtgtatca tagcggattt acacaaaaaa gtctattcac taactgcttt cagagtaact
    13261 tctatatttc cagctgtagc ttttgaatac ggacacactt tatgcgctgc ctctaataat
    13321 tcttgcgttt cttccagaga aagaccttcg atagatcctt caatctctac acctaagaca
    13381 aagctcgttt cactttctga ataaagggaa acttgtgcac tgacagtaga agccccagag
    13441 atattttttt gctgtttgat caattctaat gcactgttaa aacatgcact gaaaccagct
    13501 gcaaacaatt gttcagggtt ggttgcattt tcgacgcgtt taccaggagc tgcaatattc
    13561 aattcgaatg tacgatcggg attatgcact tctccagagc gtccgcctgt gttgatcatt
    13621 ttcgtagaat acattttttt cataaaatta ccacctttaa ttatatttta tacaattgaa
    13681 ttttacacaa tataattgtg gaaagcaaag aaatggattg gcataagata aataagagaa
    13741 agcacatgaa acagacgcgc tttctcgaat agcaaacata tacaaaaaat atcagagaca
    13801 aaaaataatc tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa
    13861 aaattacttc acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca
    13921 ttgagttagt cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat
    13981 ttcgtaatgc tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg
    14041 tatttggatg ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat
    14101 ggaatctcgt accatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg
    14161 cctcaatcag ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat
    14221 catttaatac aaaatcgatt tccgttcctt tatgcataaa gatctcctct ccttttcagt
    14281 cgaacagtta ctagattctt ttattatacc agctaaagaa aacgcattca aaaaaatagc
    14341 ggatttactt accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg
    14401 ctacgctgct gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc
    14461 gggaaaattc atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc
    14521 cgccttttgc ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga
    14581 tgtcttccaa ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt
    14641 cgatataagt catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt
    14701 ctattccaaa aataaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa
    14761 tcgaagtatt caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc
    14821 tagaagagtt gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa
    14881 tgacagacaa caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag
    14941 gaaaaaccaa gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag
    15001 ctagtaccaa cataacgata ttcaacacta aaataactat agctcggtca aagcctaaag
    15061 cagcttccag taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta
    15121 aaaacatatt gatagcgact cctaatatga ag
//
LOCUS       DACDRK010000063        15084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDRK010000063 DACDRK010000000
VERSION     DACDRK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.063"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(167..1246)
                     /locus_tag="IWJ77_002058"
     CDS             complement(167..1246)
                     /locus_tag="IWJ77_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9453645.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            1354..2805
                     /locus_tag="IWJ77_002059"
     CDS             1354..2805
                     /locus_tag="IWJ77_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9453646.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            3154..3786
                     /locus_tag="IWJ77_002060"
     CDS             3154..3786
                     /locus_tag="IWJ77_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9453647.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            4374..5813
                     /locus_tag="IWJ77_002061"
     CDS             4374..5813
                     /locus_tag="IWJ77_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9453648.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            6108..6326
                     /locus_tag="IWJ77_002062"
                     /pseudo
     CDS             6108..6326
                     /locus_tag="IWJ77_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6575..6790
                     /locus_tag="IWJ77_002063"
     CDS             6575..6790
                     /locus_tag="IWJ77_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453649.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(7007..7263)
                     /locus_tag="IWJ77_002064"
                     /pseudo
     CDS             complement(7007..7263)
                     /locus_tag="IWJ77_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(7450..8361)
                     /locus_tag="IWJ77_002065"
     CDS             complement(7450..8361)
                     /locus_tag="IWJ77_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9453650.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(8380..9384)
                     /locus_tag="IWJ77_002066"
     CDS             complement(8380..9384)
                     /locus_tag="IWJ77_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9453651.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(9381..11504)
                     /locus_tag="IWJ77_002067"
     CDS             complement(9381..11504)
                     /locus_tag="IWJ77_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9453652.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(11509..13956)
                     /locus_tag="IWJ77_002068"
     CDS             complement(11509..13956)
                     /locus_tag="IWJ77_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9453653.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(13943..14332)
                     /locus_tag="IWJ77_002069"
     CDS             complement(13943..14332)
                     /locus_tag="IWJ77_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9453654.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(14392..14895)
                     /locus_tag="IWJ77_002070"
     CDS             complement(14392..14895)
                     /locus_tag="IWJ77_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9453655.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(14908..>15084)
                     /locus_tag="IWJ77_002071"
     CDS             complement(14908..>15084)
                     /locus_tag="IWJ77_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453656.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4525 a         3032 c         2826 g         4701 t
ORIGIN      
        1 acaggtaact catttgtttg ataccgtaac tgcctcgtta ttctaaatca gtgaggcctt
       61 gataatgaac tgaggtatga tagtcttcag acgattctgg ccccaggcca gctttccaga
      121 catgaagcca tgtcccgagt gaggatcgtt atcaaggaat aatgggtcaa gccaccaacc
      181 gttcttgctt gaatgcctgg acgaatgtat catgtggtgt cgcataaccc aaaattttcc
      241 gcgggtaatc gttcatccaa cgctggatcc gcaaaatctg gttctcgctc acctcactaa
      301 tcgggcttcc cttggggacg aaacggcgaa ttaatttgtg ctggttttcg ctcgtcccac
      361 gctcccaaga agcatagggg tggctgaaat agacgtcagc tacatcttgc agagcatcgt
      421 gtagcccaga aaactccgag ccattgtcgg atgtaacggt cttaaataat tgatcgaagt
      481 tagcgccagc ccgttctctt agctgttgaa ccgcttggtc caccgattcg gtgtccttcc
      541 cgttcaactt taggatgact tcaaagcgtg tctgccgttc agccagcgtc agcagcacgg
      601 catccgactt ggtcttgttt cccactaccg tgtcgatttc ccaatgaccg aacgtttccc
      661 gtgtttcaac ttctttaggg cgttcttcaa ttgacgtccc gagaacacgc ttgttgggac
      721 ggtgtcctgg gcggaccgct ttgattttac gggacagttt ttcaagcaag tccatgttct
      781 ttgttcgcat gattccgcgg tcaatccatt gataaagagc ggttgtgcca ggaatgatgg
      841 cacaatcgaa cagctcgtgt tttctggcaa atccgaccac gacgtccggc gaccattttt
      901 catttaacat tttgtcatct gcccaatcaa tgaaggcatt tgtgttcgcc catttggggc
      961 gtctgccaca gttcagtcgt tgcttttcat agaaggcctg tcctgcatca gcgtcataaa
     1021 cagaatagta ggtgtcatac acttttccat tttgcgtttg gcgttttaac tgggtgatgg
     1081 ttccgcgatc aatctcattg tttatggttt gcggaacacg tcccagcact ttggcaacct
     1141 gacggttgga atagccttcc tttttcagga tagcgatttg gcaacgttct gagtaggaaa
     1201 gatgttttcc cttgcgtgaa actgtgttat catgggtttg cgtcatgtga attcatcctt
     1261 gtctattgag agttgtggta acttcaatat aacatggaat tcacgtggcg ttttttttat
     1321 tttaaatggc taagttcatt ataaaatccg ccaatgaaaa aatctttaaa acttgcatca
     1381 gcagttatat taatgtccca actccttata gcaacacctt tccatgtgtt ggcaagcgaa
     1441 aattcaacat acaaaattgc aaaagaaaga accgagacga ctgaaatgag taattccgat
     1501 gtaacagcag agagtagtga acttttgaca acttcatcaa gtgaaagtag tacgacttca
     1561 aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa tgccgagtct
     1621 tcaacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttcg
     1681 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
     1741 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
     1801 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
     1861 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
     1921 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
     1981 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
     2041 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
     2101 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
     2161 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
     2221 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
     2281 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
     2341 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
     2401 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
     2461 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
     2521 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
     2581 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
     2641 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     2701 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     2761 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     2821 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     2881 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     2941 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     3001 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     3061 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     3121 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     3181 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     3241 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     3301 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     3361 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     3421 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     3481 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     3541 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     3601 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     3661 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     3721 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     3781 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     3841 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     3901 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     3961 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     4021 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     4081 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     4141 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     4201 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     4261 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     4321 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     4381 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     4441 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     4501 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     4561 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     4621 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     4681 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     4741 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     4801 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     4861 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     4921 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     4981 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     5041 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     5101 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     5161 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     5221 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     5281 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     5341 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     5401 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     5461 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     5521 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     5581 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     5641 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     5701 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     5761 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     5821 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     5881 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     5941 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     6001 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     6061 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     6121 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     6181 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     6241 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     6301 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     6361 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     6421 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     6481 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     6541 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     6601 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     6661 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     6721 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     6781 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     6841 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     6901 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     6961 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     7021 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     7081 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     7141 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     7201 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     7261 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     7321 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     7381 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     7441 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     7501 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     7561 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     7621 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     7681 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     7741 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     7801 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     7861 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     7921 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     7981 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     8041 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     8101 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     8161 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     8221 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     8281 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     8341 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     8401 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     8461 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     8521 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     8581 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     8641 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     8701 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     8761 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     8821 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     8881 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     8941 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     9001 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     9061 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     9121 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     9181 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     9241 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     9301 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     9361 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     9421 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     9481 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     9541 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     9601 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     9661 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     9721 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     9781 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     9841 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     9901 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     9961 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
    10021 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
    10081 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
    10141 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
    10201 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
    10261 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
    10321 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
    10381 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
    10441 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
    10501 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
    10561 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
    10621 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
    10681 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
    10741 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
    10801 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
    10861 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
    10921 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
    10981 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
    11041 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
    11101 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
    11161 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
    11221 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
    11281 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
    11341 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
    11401 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
    11461 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
    11521 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
    11581 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
    11641 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    11701 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    11761 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    11821 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    11881 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    11941 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    12001 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    12061 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    12121 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    12181 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    12241 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    12301 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    12361 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    12421 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    12481 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    12541 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    12601 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    12661 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    12721 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    12781 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    12841 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    12901 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    12961 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    13021 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    13081 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    13141 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    13201 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    13261 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    13321 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    13381 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    13441 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    13501 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    13561 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    13621 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    13681 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    13741 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    13801 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    13861 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    13921 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    13981 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    14041 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    14101 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    14161 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    14221 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    14281 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    14341 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    14401 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    14461 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    14521 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    14581 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    14641 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    14701 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    14761 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    14821 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    14881 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    14941 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    15001 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    15061 aattgccagt aagactaagg attg
//
LOCUS       DACDRK010000064        14756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDRK010000064 DACDRK010000000
VERSION     DACDRK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.064"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            142..1881
                     /gene="yfcC"
                     /locus_tag="IWJ77_002072"
     CDS             142..1881
                     /gene="yfcC"
                     /locus_tag="IWJ77_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9453657.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2038..3222
                     /gene="iadA"
                     /locus_tag="IWJ77_002073"
     CDS             2038..3222
                     /gene="iadA"
                     /locus_tag="IWJ77_002073"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9453658.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3333..3536
                     /locus_tag="IWJ77_002074"
     CDS             3333..3536
                     /locus_tag="IWJ77_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9453659.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3637..4299)
                     /locus_tag="IWJ77_002075"
     CDS             complement(3637..4299)
                     /locus_tag="IWJ77_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453660.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4312..5814)
                     /locus_tag="IWJ77_002076"
     CDS             complement(4312..5814)
                     /locus_tag="IWJ77_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9453661.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6362..8041
                     /locus_tag="IWJ77_002077"
     CDS             6362..8041
                     /locus_tag="IWJ77_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9453662.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8168..8647
                     /locus_tag="IWJ77_002078"
     CDS             8168..8647
                     /locus_tag="IWJ77_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9453663.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8866..9546
                     /locus_tag="IWJ77_002079"
     CDS             8866..9546
                     /locus_tag="IWJ77_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9453664.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9554..10885
                     /locus_tag="IWJ77_002080"
     CDS             9554..10885
                     /locus_tag="IWJ77_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9453665.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10900..11862
                     /locus_tag="IWJ77_002081"
     CDS             10900..11862
                     /locus_tag="IWJ77_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9453666.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11973..12311
                     /locus_tag="IWJ77_002082"
     CDS             11973..12311
                     /locus_tag="IWJ77_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9453667.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12390..13097
                     /locus_tag="IWJ77_002083"
     CDS             12390..13097
                     /locus_tag="IWJ77_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9453668.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13200..14681)
                     /locus_tag="IWJ77_002084"
     CDS             complement(13200..14681)
                     /locus_tag="IWJ77_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9453669.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4695 a         2539 c         3166 g         4356 t
ORIGIN      
        1 cataggagaa aaaaacaaaa tagaaaaaag tgattttcta ataaattatt catgtggaaa
       61 tgtcattttt actgtataat tcatttgatt atccattttt tattcatcaa aattactgaa
      121 aaaagaaagg aagaaataaa tttgaagaaa acaaattctt caggaaaacc gaaacgcgac
      181 ttcactaaat tatcgacacc ccatacgtat gtcattattt ttggtgtagt catctttgca
      241 tggatactga cttttgtcgt tccagcagga aagttcagca cgcaagatat tgaatacaaa
      301 gatgcaaatg gcgaaacaag tactcgtact gttttacgtc aagattcctt ccgttatgct
      361 tacgagctgg ataagtcgta tgtgtttgat caattagaag aactgcaaga tcatccagca
      421 gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa aagtcattgc tgacggagaa
      481 aaaaatctta cgcaggaaaa actagatgaa atctccttga cagatgatgt gctttacgat
      541 cagtacggcg aaaatatcta tgatacttct aaaaaattgc ataaaacagc caaaatatgg
      601 ggaacagatg attttggcgg ctttggtttc ttaaattttg tttttgaagg actagtatct
      661 ggagataagt atggatctgc tgtagggatc gctgcattga tccttgttgt tggaggtgct
      721 tttggtatta ttatgcgtac cggagcgatc gacgcaggta tctatgcgtt tatcagtaaa
      781 actaagggac tggagcgttt agctttaccg ctattattct ttgctttttc attcggtggc
      841 gctacattcg ggatggcaga agaggtaatt ccattctcaa tggtgatggt tccatttgtc
      901 attgcgttag gatatgactc gatcgtagca gtaaccgtga cttatgtcgc atctcaagtc
      961 ggaaatgcga cctcttggat gagcccgttt agcgtagcgg tggctcaagg gattgctgga
     1021 atccccgtgt tgtctggtgc gacattccga ttgattatgt gggtagttgt tactgcatta
     1081 gccgctggtt atatgatgat atatgcagaa aaaatccgta aaaaaccaga aagttcgttg
     1141 acttacaaat cagatgatta tttccgttct catatcaaaa agacatctga tgaaaataaa
     1201 aaattcatgc ttgggcataa attgatctta ttagagatgt tagttgtcct tgtctggatc
     1261 gtttggggcg tgactcaaaa aggctattac attccagaaa tcgcttctca attctttgtg
     1321 atgggattag ttgctggaat cattgctgtt cttttcaaat tagatggaat gaaagtcaat
     1381 gacatcgctt cttctttcca atctggtgca gctgatttag ctggtacagc aatcgtagta
     1441 ggtatggcca aagggattct tcttgtcttg ggaggatctg atgcatctgt tccttcagct
     1501 ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg gtgttccagc tgtcattggt
     1561 gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg ttgttacatc aaattcagga
     1621 caagcagctt tgactatgcc aatcatggct ccactggcag atttagtagg tgtatccaga
     1681 caagtagcag ttcttgctta tcagcttggt tcaggtttta tggacgcttt tacacccgta
     1741 tcagccagct tgattggtgt gctaggtgtt gcacggatcg aatgggcaaa atgggcaaga
     1801 ttccaaatca agatgcaagg attcctgttt gttttaggaa ctattttcat catgattgcc
     1861 attgcaatag gtctgcagta gtactggaga cctgtaaata aaaaagtaat ccaaacagta
     1921 gataaggact ccaagaaccg tcattttacg gtaaaatcaa aggagtaaag atcctatcgt
     1981 ttggattatt ttttaagatc ctatcatcag ttatacttaa agaaagagga aaaatgtatg
     2041 aaaatcattc gacaaattga agtgtttgca ccagattact taggaaaaat ggatgtactg
     2101 attgccggtg ggaagatttt ggctgtagaa gaacagctag aaggcggcta cgaaggagtg
     2161 gaagtagaag aactttcagg tgagggaaaa gtactaaccc cgggtttcat tgactgtcat
     2221 ttccatattt taggtggagg aggagaaggc ggttatcaaa atcgaacgcc agaagtcaca
     2281 ttgagccaat tgactacagc tggtgtaacg acagttgtcg gttgtttggg aacagacgga
     2341 gaaggtagag atatgactgc gttgatcagc aaagcgaaag gtctggaagc agaaggaatc
     2401 tccacttatg tatacgaagg ttcgtaccga ttgccggtaa aaacagtaac agattctatc
     2461 atcaaagact ttttgacaat tgataaaatt atcggaatcg gagaaatcgc agtatccgat
     2521 caccgttctt ctcagccaag ttttgaagag ttcgctcgtg cagtagcaga tgctcgtgta
     2581 ggaggcatgt tatccggaaa agctgggatc atcaatgtcc acttaggcgg aggaaaaaga
     2641 aagctagagc tattgacacg aatcgtagaa gaaacagaaa ttccgatcac acaatttttg
     2701 cctactcatg caaatcgcac accagaatta tttgaagctt gcgtggcatt tgccaaacgt
     2761 ggtggcacga ttgacttcac tgctagcgaa gatcctgatt tttgggagaa gacagatggt
     2821 gaagttcgct tcagcaaagc actgaaacga ttgatcgagg aagatgtttc tttagataat
     2881 ttcacgatga cctcagatgg acaaggaagc ttgccttatt ttgatgaaaa caatcatttc
     2941 ctagggttag gagtaggaag tgccaaggca ttgctagtag ggatcaaaga agctgtgcaa
     3001 aaagaaagta tccctttgga aatcgcctta cgagctataa ccagtaatcc agcacgtata
     3061 ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg atgcagatct gtgtatcttg
     3121 gataaagaaa cgcttgatat tgatacagtt atcgctaaag gagaaataat ggtccaagaa
     3181 aaagaagtca aagtatgggg gactttcgaa aaaagttttt aatcaacagc tctaactatc
     3241 ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg ttgatgttac cctgaaggta
     3301 gatcaataaa caaactaaga ggaggaagca caatggcaga tttaaaagga cgttctaaag
     3361 atatgaaaga taaagtagtc ggtgaaggaa aagaagcata cggcaaagca acagacgaca
     3421 agagcaaaga aacagaaggg aaagcacaat cactaggtgc tgacatcaaa gaaaaagctc
     3481 gaaatttagg cgacgaagtc aaagacaagt tttctcgaaa agacgaaaaa aaataaaaac
     3541 cacttattac taattgaatc aagcaaataa aaaatccgaa ccatatctgc gagacagata
     3601 tggttcggat tttttatttg atcattatcc tgttttttaa acttcgattt tttgaaattt
     3661 tttatttttt agttcaaaga cttcatcaca gctttttgct acttcaatct catgagtcac
     3721 gatgataata cagcgatttt gttcatgggc gatttcttgg aaaagtttga cgatatcttg
     3781 tgaagttgtt tcatctagat ttccagtagg ctcatccgct acgattagtt gatgttcaca
     3841 gcagatagca cgaacgatag cgactcgctg ctgctgacct cccgaaagtt tggtcacctg
     3901 tttttttgct aatatttcat caattcctac acgagccagt cgatctaaag caacttgtcg
     3961 ataattctct tgcttggtct tagtgatttc cattgctgct atgacgttgt ctagagccga
     4021 taaataagga agcaagttgt aagcttggaa aataatcgag acatcatttt tacgataact
     4081 tcttaacccg attttgttca gtgaacgatc ttgatagtat acctttcctt cttttggtgc
     4141 atctagtcca gaaataagcg aaagaaaagt cgtttttcca gaaccacttg ctcctaaaat
     4201 agcgtacatt tttccagctt cgaaattcag ttctacatct ttataaagcg catcttgcgg
     4261 atcttgatac caaaatccca attgttctgt ccgtaacatc aaattcctcc tttagtttaa
     4321 taggattttt cttgggttta atcttaatat ccctatagaa gctaaaaaga cagaaaacag
     4381 actgattcca aatgctactc cggctaatag tcccaattgt gcaggtttga cattgatttc
     4441 caattcgctg acttgatcag aaacagcaaa tggattattc gtggttcctt gttgaccatc
     4501 gcgttgaccg ccttgttctt gtctgttacc tggcatttgt ccaccgccag gagcttgctg
     4561 gttttgtgtc gtggtttctg tctgttggtc aagtagctgc tgtcccacgg cattcgcgac
     4621 gacgtttccg ctaagcgaag caactcctaa tgcaagaatc atacaaatag ccacttcggt
     4681 aaacaattgg agaatgattt tacttctgga ttctcctaaa gatagcaaga cgcctaactc
     4741 atgccgacgc tcccgaatgg taatcataat gatcagagta agtatgataa taccggctac
     4801 agccactagt aaaacaacat ttttagaaaa acttgccacg ttgtttaaag gttccaacat
     4861 tgactgatac atggaatcat ttgattgtaa gctaaatgta tctgtgtcga tcagcttctc
     4921 agcttctttg agaaactcat ccattttatc tggatcagat agagtgtaaa tagcagaatc
     4981 tatcgtgtta tcgtcagatg tcccatttag ttcatttgca aaagtgtaag aagtgtaaag
     5041 tgtattcgac ggattcatga agttgaattt catacccata ctgccgcttg tttcgctgga
     5101 ttcataaatt cctttgatcg tcatttcata agtggtatct tcatcttcag tactcgtgat
     5161 tgtgaaagta tctccgactg ccaggtcatt tgtttctgct agagtagaat caatgactac
     5221 attgtttgtg cctttgtcgc tgtcatctat tccttcacca tccgtgattt ttgctgttcc
     5281 atcagaaaaa ttactatttt gtgcagttgc tgacacacca ctgatttgaa agtctgcttg
     5341 agtcatttgc ggattcccgc ccattcctcc agcaggttca ccgcctgcat cagtagtttc
     5401 tgaactgtct tcacttgaat cagaactagt gatggcagtt attccatctt ttgccatagc
     5461 tgaggcggaa ctttcaaagg aataactctt tacattatct aattcagcta ttttttccgc
     5521 gtccgttaag gaaactggtg taagagaaaa actgcctgga tctggacgtt cttcagtggt
     5581 agaaccgctt gaagaagttt gttcctgttt tttaaatgaa gcttctcgat tggcacttaa
     5641 agtcactgtt gctccaactt ctttttgtgc ttgattcgct gcttcttctg cagcgcttcg
     5701 aatcgtgagg ccagctagca caaaaataag aatggctgtg aacacggcaa acaataacaa
     5761 tgaacgtccc cattttactt ttgtactttt ccatgcacgt ttcataaaat tcatattttg
     5821 catccctcct agtagatgca tttgataata gacaacctat cttaaagcta gcttaaatct
     5881 tatgctgttt gcacaccatc aacatgaggt tctgtatata taaaaagtca ctatagttat
     5941 tgcaaaaaat gtggtaaaat gaaaaaagta tgtttcaaac cgacgaagaa gaaatgaaag
     6001 aggaatagca aaagaaatcc tagatgctac agagggattt ttacgtgcat agttgttcta
     6061 acaattgctg atactagcga tttagtttat ttgttgtata ataatgggga acagctgaat
     6121 acttatataa gtattctggc tatagcgttt cgcaaaagat ccattttgtt aatgaagtat
     6181 atccaaaata aaatgcccat ccaactgtca gaacgcggat gtgctggtaa ttttcctgct
     6241 gattctccat aagttgagaa atagtgattt tgcatatgct tcattggaat ggctcatata
     6301 tttagataaa gaagaaaatc gacatcttta aacttggatg tcataagaag aggtgaagtt
     6361 tgtgagtacc attaaaatta tcccattagg cggcgttcgt gaaaatggaa aaaacatgta
     6421 tgtcgcagaa gtgggcgagg atatttttat tttagattgt gggctaaaat atcctgaaaa
     6481 tgagttgttg gggatcgacg tcgtaatccc tgattttacg tatttagaag aaaacattga
     6541 tcgtgtagct ggtgtctttt taacacatgg acatgcagat gcgatcgggg cacttcctta
     6601 cttgttgtca aaaatttctg taccagtatt cgggacagag ttgacgatcg agttagcaaa
     6661 attgaatgtc agtcgtaatg aagcagccaa aggatttaag gatttccatg tggtagatga
     6721 gcatacagaa atagactttg gtgaaacagt agtgagcttt ttccgtacca ctcatacgat
     6781 accagattca gtggggatca atctgaaaac aaaagaaggt agtatcgttt atacgggaga
     6841 ttttaaattt gaccaaacgg ctatcccaat gtatcaaaca gattatgcac gattggcgga
     6901 aatcggcaaa gaaggagtct tagccttact aagtgaatcg gcaaatgcag aaaatcctca
     6961 gcctgtagct tctgaagccc aaatagcaga tgaagtattt gatacgatca aatattggga
     7021 agggcgtatc atcgtcgctt gtgtagcaag taatttgcaa cgagtgcaac aagtattgga
     7081 tgcagcagaa aaagcacaac gaaaagttgt cttgactggt caagatttcg aacgaattat
     7141 tcgcacagcg atgcgtttag aaaaacttca attaccaagt gaagatctgc ttgttacgtt
     7201 gaaagacatg aaaaactatg atccagaaga actgatcatt ttagaaactg gccgaatggg
     7261 tgaacccatc aagtctctgc aaaaaatggc aaacggtaca caccgtaatg tcaagattga
     7321 agaaggagat ttggtttata tcactactac gccaagcatc gcgatggaaa ccatcgtggc
     7381 gaaaacggaa gatatcattt atcgagctgg tggaacagtg aagttgatct ctgagaatat
     7441 gcgcgtttct ggccatgcca atcctaatga cttgcaatta atgatcaatt tgatgaaacc
     7501 aacatatttt gtgccagttc aaggagagta tcgtgaatta gctgctcatg cagatttggc
     7561 tcatgcagta gggatgccat acaaaaatat ctatattact ggacgcggag acgtgctgga
     7621 atataagaac aagcgtatga gtgttgcagg aactacaagt gcagaaaatg taatgattga
     7681 tggattaggc ataggtgata tcggaaacat tgttttacgt gatcgaaaaa tcttgtcaga
     7741 agacgggatt ttcgtagcgg ttgtcactat cagtcggcga gaaaagaaaa tcatttcaaa
     7801 acctcaaatt acttcacggg gattcgttta cgtaaaagct agccgtgatc tgatgaaaga
     7861 aagcagcaat atgattgaaa caatcgttga gaaacattta gaaagcaatg attttgagtg
     7921 gagcaaatta aagcaagaca tccgagatca attaggccgt tacttatttg aacaaacaaa
     7981 acgccgccca gtgatcttgc cagtcattat ggaagctact caacgtcgaa ataaaaaata
     8041 aacaaaaaga gtaagatgaa cctctctata gagtgctcgt cttgctcttt ttttgcgaaa
     8101 aacaaataat ttttgaatac agcatccctc gctttataat aaacgataga ataaagggag
     8161 tgaatcaatg atgagtcaaa caaaagtcga acgttattta gaagaacaaa agatcagcta
     8221 tcaaccactt gattttagtc atgtggtaga agaatctttc acagaagaac tacaaaaaag
     8281 agggattgat ccagctttga tatgtaaaac tctcgtagtg aaaggaaaca aaactggggt
     8341 gatcatcgca ttgattcctt tgaacgatca cttagattac aaaaaaacga gacaagtgac
     8401 gaaagatcgt aaagttggtt ttcctggaat ggattttgta ttaatgcata caggatatcc
     8461 ccacggagca aacaccccta ttggaataaa actcgcacat cctgattatt gttttttagc
     8521 agacagttct ttaaaagaaa aagaagaagt aattgtgtca agcggtgaaa tcgggagaag
     8581 ccttcggata aaagtagccg atttagaaaa gatcgtccat cctatttacg ctgatttaat
     8641 tagttaaggg acgagaagag aagctaagca gagcagagac ctcaaacttg agattatatc
     8701 tgcttggtac ttggtgcatc ctgtattatc tgttacaatt gctttgtaca tactgtacta
     8761 tcaatccaat gaaaatatat ttagcagcta tattttttca ttgatgtgca agtgatgaaa
     8821 tcgtttttaa taaaaaagat acagagaaaa cgcgaggtgt aaaatatgaa aattacgtat
     8881 catggacatt cctgtttaac aattgaaatg gaaaatggcc aacgcgtgat gattgacccg
     8941 tttattacag gaaatccatt gacagactta actgcagaag aagtaaagac agattggttg
     9001 ctgattactc atggtcatag tgaccacata ggagatatga ttcctattgc aaaagaaaat
     9061 caagccacaa ttatcagtat tgttgagatt gctgattatg cgaacagcag aggagtagat
     9121 tcattcggta tgaatatcgg tggaaaacat gcgtttccat tcggcgtggt aaaattcgta
     9181 catgcacagc atagttcaag ctatgaagta gacggtatcg ttcagtatat gggcgagcct
     9241 agcggaatta ttattcaagc agaaggaaaa acgatttatc atgcagggga tactgcttat
     9301 ttcagtgatc taggattgct tgcagaagaa ttcgatattg atgtcgcttt cttgccgatt
     9361 ggcgataact atacgatggg gccagaagat gcagcaaagg ctgcgcaaac aattcgtgcc
     9421 aagaaagtag ttccaatcca ttacaatact ttccctgtca ttgaacaaga tcccgaatct
     9481 tttgtagagt tactggaaga tagtgaagga aaaataatga gcgtctcgga aacgctggaa
     9541 gtgtaggcag aaaatggcga caaaacatga tttgatttta gaatatattg aaagtttacc
     9601 tgttggcaat cgaatttctg taagaagcat cgcaaaagaa ttgaatgtca gtgaaggaac
     9661 agcgtaccgt gcaatcaaag atgcagaaaa tgtcggacta gtttcaacaa ttcaacgagt
     9721 agggaccatc cgtatcgaac gcaaattaaa aaaacatatt gaacgtttga catttggcga
     9781 ggtcgtcagg atcattgaag gggacgtttt aggcggggcg gcaggattag acaaggtctt
     9841 gaataaattc gtcatcggcg cgatgactaa acatgccatg gaacgctata ttacgccagg
     9901 atcactaatg atcgtcggca accgggcgga agtccaaaaa ttagcattgg aagatggcgc
     9961 cgcagtattg atcacaggag gattcaatac gactgctgag attgcaaagc tagctgatga
    10021 tcttgaaatg ccgatcttac gcacctctta tgatacattt acagtcgcaa ccatgatcaa
    10081 ccgagcgtta agcgaccaat tgatcaaaaa agatatcatg cttgtcagtg atatctatat
    10141 gccagctgaa aagactcatt atttgcagca gacagatacg atttctgatt atcagcgttt
    10201 gtcagaagaa acgcagcatt cgcgttttcc agtcgtcaac cgccatcatc gtttgatggg
    10261 gattgttacg gcaaaagatg ttttagggaa atcgccaaac cagatcatcg atcgggtgat
    10321 gacgaaagaa ccaatcagtg taaagaaaac aatgagtatt gcctctgtta gccatcaaat
    10381 gatctgggat gggctagaag tcatgccggt cgtttccgat gacttgacat tggaaggctt
    10441 gattacccgg caagatgtaa tgaaagcgat gcagcttgtc cagcgacaac cgcagattgc
    10501 agatacgatt tcggatcaga tatctggaaa tatccatacg attgatactg accgtgaagg
    10561 aaatcgtttg gaaaacccta agttcaaatt cgtagtagca ccacaaatgg tcaatggcgt
    10621 tggaaccatt tcttttggcg tacttagcga aatcatttct gatgtcgcac aaaaaacaat
    10681 ggtgatgaac caaaaacgaa atatcttgat tgaacaagtg aacctacatt atttgcggct
    10741 gatccaattg gaaagcgaat tagacattcg tccacgtgta ttggaaatcg gcagacgttc
    10801 cgctaaactg gatatcgaag tgtttattga aaacaccatc gtttccaaag caatcgttgt
    10861 ttgtcaagta atggaacgtt cttagaaagc aggagtgaaa tgacagtaca gcaagaaata
    10921 gtagatacaa taaaaaaata cgataaaatc attatccaca ggcatttgcg ccctgatcct
    10981 gatgcattag gatcacaagt aggattagcc gaactattac gggaaagttt cccagaaaaa
    11041 gaaatacgtc aagtaggtga aacgattgaa gggcttcgct ttttagcaga aatgcaagaa
    11101 gttgaagatg tcttctacca aggagcgcta gtcatcgtga ctgatacagc taattcaccc
    11161 agaatcagtg atcaaagata tcagttggca gaaaaactga tcaaaatcga ccatcatccc
    11221 aatgatgacc cttatggaga tcttgtgtgg gtaaatacaa aagcaagtag ctgtagtgag
    11281 atgattgcag aatttgcttt gatgtttcct gaatctttaa agataaacgt taaggctgca
    11341 cggttacttt atgcaggaat cgtcggagat acagggcgct ttttgtatcc ctccacgacg
    11401 tcccgtacat tagagattgc ttcgatattg agaaattatc catttgatgc aagtgctttg
    11461 aacagagaga ttgaacaaat gccgatgaaa gtcgctaaac tttccggtta tctttatcaa
    11521 aatatccagg tggatgaaaa tggtgcagga agaataatcc ttccgcaaga aattcttgat
    11581 aaatatggaa tcgatgattc agagacagct tcgatcgttt cacttcctgg agtgattgac
    11641 gaagtgctgg cttgggggat ttttgttcag cagccttcag ggtattaccg cgtacgttta
    11701 cgttctaagg ggccgatcat caatgaattg gctaaacgcc atcatggagg aggccatcca
    11761 ttagcaagtg gtgcgaatgc aaaagattta gcagaagtag aggaaatcta taaagagatc
    11821 caagatatat gccaatcgta taagagcaga aaaacagaat gatacttcgt tcgaaaacgt
    11881 gccttttgta gtaaaaatca caaatgtatc ttttcaagat cttctttttc cgttacaata
    11941 gtgctcacta aagattttaa aggagaaaga agatgacatt accaaattgc ccagaatgtg
    12001 gctcagagta cacatatgaa gatcgaggct tgttgatttg cccagaatgc gggaacgaat
    12061 ggaacaaaga agaacaagaa actgtcatag aagacggatt agtggtatta gatgcaaatg
    12121 gaaaccgtct gttagagggc gatagcgtga cagtgattaa agacttgaaa gtaaaaggtg
    12181 cgtcaggtgc cattaaacaa ggaacaaaag tcaagaacat ccgtttagta gaaggcgatc
    12241 ataatatcga ttgcaaggta gaaggatttg gaccgatgaa actgaaatcg gaatttgtga
    12301 aaaagaatta aagaatattc aaggtctttt cacattcttt tcctactact aatgataaaa
    12361 taaagtaaac gaaaaggagt gaaacgaaaa tgtatggtta tggaatgtat ggttttttag
    12421 atccgacatt tcttttggtc attcttggat tagttatttc cggaattgcg tcagcgtacg
    12481 tcaatagtac tttccgaaaa tacgatcaag tgagaagcaa aaacaatgtg actggtacac
    12541 aagcagcaca atatatatta caaagtcaac agataaataa tgtcggtgtt caacaaattg
    12601 ccggagatct cacagataac tacaattcag gtaataagat gttgagtttg tctcaagcaa
    12661 ctgcacagtc tacttcagtt gctgcgatag gtgtagcagc tcatgaatgc ggacatgcgg
    12721 tacaagatgc ttcgggttat gtcccattga agattcgtgc agcacttgtt ccagcagcta
    12781 actttggttc gatgatttcg ttccccttga ttatggtagg tgtattgctt agctggaact
    12841 ctacgttaat caatatcggg attttcgctt tttctttagc attacttttc caattggtga
    12901 cattaccagt cgaatttaac gcgtctcggc gagcaatcca aattttgagt gaaggcggac
    12961 tactgacgga agaagaagta ccgatggcaa gacacgtatt atttgcagca gcactgactt
    13021 atgttgcagc agcgctttcg acattcctgc agctgttgcg cttgatctta ttgttcggcg
    13081 gcaaccgaag agattaaaat aaagaagttg tgaaatcagc gctttgatct gagctaatac
    13141 cgagagttag ctgattgaat accatttatt tggaataaga ggatgtgaca agaattttgt
    13201 cacatcctct ttgttttgtt ctcataacct tttttcatat acagttcatg aaccgctttt
    13261 attaacttgc caaataacac tgtattgtgc gttgtatcca gatagaaagt ctccgtttca
    13321 tctgaaattt tctcatgctg atctgtaatg atcaaatcag catgttctga atatgcggta
    13381 attttaacag catctttgcg aaataactgc tgcaaatggc tttgaagatg ataatttcct
    13441 gtaaactgtt tggttatctg caaatggatg agtaactgct ctttcgtttc tgtttgtagt
    13501 aagtgttgga tcaattgact aaggtattga ctgatttggt ttctttcccc attttttgaa
    13561 aaaattgtat cgattttttc tcttaatttc ttttcctgta taaaagaact agaatgattg
    13621 gacgtggctt ctggaaagca aagtgtagaa aaactactgc tggattcacc caacagttcg
    13681 aaaaaaacaa ctgccaacac aagaaaataa aaataaattg gtattttatt ttctaaatgg
    13741 ttgagaggaa actgttgagt taagtaagtc atcaattgac cgacttgtcg acaaataagg
    13801 tttttgcttg tggaaaataa aaaaccaatc tcctgttttt gtcgaaaaga gatttgattt
    13861 ggaaaaaaac aatagaaaaa ataattaaag taggctctct ctgtttctgt ctcttcacaa
    13921 gggcgactca ctgaaaaaga agtaaagtat tctgaataat catgtgtatg ctgcaataag
    13981 tccatcaatt cagtgatttc agaagaagtt tttggaaaat atgcctgttg tttgtttcgt
    14041 aatatgaata aagttaacag caagtagata tttttttctt gttttgaaat tttttctatt
    14101 tgctcatctg tactgttcat ttttagtgca tttaattcaa gtgaggaaaa tggccaatcg
    14161 ttacctccaa atgtatctga aagaaaaaag taaagataga gacggataga taactcattt
    14221 ccttcaagag tacagcaatt ttttacagat aggatccgta aatggaattt ttttaaggaa
    14281 tgattgagct gttttgtcag tttttcgaga taggagggcg atatataaag ttgttcaagt
    14341 aaatattttt ttggtacagc agtttgtgga taattgatta gtaacatcaa atagttaaaa
    14401 cgattggatt cttttaggta gcataatttt aacttagaaa aaacagctaa ctttttagtt
    14461 tgatcggttt ctttggtttg gatctctcct ttatgattag aataaataaa ttcatcaatc
    14521 aataaaaatt gcttgagtga tacatttatt tttcggatca gttctttgat tgtccgtttt
    14581 ggaatatgta agtcattgga taattcttgt aaagaacaat tatttttttg aatgaccatt
    14641 tgtagaacaa gtaactcttt ttttcttgct tcaattagca taaatacctc ctttttattc
    14701 tgtttttgta tatagttaga caaaaactgt gagtggaaag tcatttcagt ctgtgt
//
LOCUS       DACDRK010000065        14412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDRK010000065 DACDRK010000000
VERSION     DACDRK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.065"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..510
                     /locus_tag="IWJ77_002085"
     CDS             1..510
                     /locus_tag="IWJ77_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9453670.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            603..1765
                     /locus_tag="IWJ77_002086"
     CDS             join(603..879,879..1765)
                     /locus_tag="IWJ77_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9453671.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2045..3025
                     /locus_tag="IWJ77_002087"
     CDS             2045..3025
                     /locus_tag="IWJ77_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9453672.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3147..4565
                     /locus_tag="IWJ77_002088"
     CDS             3147..4565
                     /locus_tag="IWJ77_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9453673.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4636..6105
                     /locus_tag="IWJ77_002089"
     CDS             4636..6105
                     /locus_tag="IWJ77_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9453674.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6115..6582
                     /locus_tag="IWJ77_002090"
     CDS             6115..6582
                     /locus_tag="IWJ77_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9453675.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6618..7490
                     /locus_tag="IWJ77_002091"
     CDS             6618..7490
                     /locus_tag="IWJ77_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9453676.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7571..10105
                     /locus_tag="IWJ77_002092"
     CDS             7571..10105
                     /locus_tag="IWJ77_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9453677.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10261..11034
                     /locus_tag="IWJ77_002093"
     CDS             10261..11034
                     /locus_tag="IWJ77_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9453678.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11027..11818
                     /locus_tag="IWJ77_002094"
     CDS             11027..11818
                     /locus_tag="IWJ77_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9453679.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            11828..12319
                     /locus_tag="IWJ77_002095"
     CDS             11828..12319
                     /locus_tag="IWJ77_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9453680.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12337..12747
                     /locus_tag="IWJ77_002096"
     CDS             12337..12747
                     /locus_tag="IWJ77_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9453681.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            12751..14151
                     /locus_tag="IWJ77_002097"
     CDS             12751..14151
                     /locus_tag="IWJ77_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9453682.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14174..>14412
                     /locus_tag="IWJ77_002098"
     CDS             14174..>14412
                     /locus_tag="IWJ77_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9453683.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVF"
BASE COUNT         5016 a         2031 c         2921 g         4444 t
ORIGIN      
        1 ttggtacaag gaatagaaag aggtgtttat gtggttgata aaattcagtt taatcaagtt
       61 atagaagaat tcatcaaagt aaataattta atgacccata ttcaaaaacg accaatcaga
      121 ataactaatg atctaaaact cagtacgagt atgattcatt tgattgatgt gattggaaat
      181 tatcctgaaa ctagtattac ggaattagct gatcgattag gcgttaccaa aggagctgta
      241 tcccaacaga ttcccacttt agagaaaatg aatcttataa gcatttctca aaaaaaagga
      301 aataagaaaa ctaaacttgt atcgttaaca aaatatggtc gaaaggtgta cgaatcacat
      361 aattatttac atgaagagct ttatagctca ataaatgaag cgttagaatc attctcacca
      421 gaccaaattt caaccattaa acagattcta gaaaaagtat cttatacaat caatgaatat
      481 caagtaagtc tgacaagaaa ggaagattga catgaaaaaa atgatttttg aatcggctac
      541 actagactag acagaaaaaa taaggtgtgt aaaataaatg aaaacacact ggaggaattt
      601 ttatgtcaag acgtcaacgt agaacctatt caaaagaatt caaacaacaa gtcgtcgatc
      661 tttatctcgc aggaaagccc cgagctgaaa tcattcgaga ttatgagctg actacctcgg
      721 cctttgataa gtgggtcaag caagcgcaaa ctactggctc gtttcaggaa aaagataact
      781 tgatccctga acaagtagaa ctaattgcat tacgaaagaa aaataaacag cttgaaatgg
      841 agaatgacat tttaaagcaa gcggcgctga tattgggacg aaaagacaag taattgatac
      901 caataagcac aaatactcaa tatcagcgat gtgtaaagtg ttaaacatct cttgccaaac
      961 ctattattat caagccaaac cagttgaaga tgaatcagaa ctggaagaac tcgttcaaga
     1021 agaatttatt cgcaatcgaa aagcttatgg taccaggaag ctgaagcagt gcttggccaa
     1081 tctaggtctt caagtgagtc aacgcagaat cggccgtatg atgaaacagc gtggcttgcg
     1141 ttcaacatac acattggctc atttcaaatt ccattcttct acttgtaacg aagccaagac
     1201 cgccaatatt ctagacggaa ccttcctaca ggaaaagtca ttagaagcga ttgtaaccga
     1261 ccttacctat gttcgtgttg ggaagaaatg gcagtatatc tgcctgattc ttgatttgtt
     1321 taatcgagaa atcatcggct attcatgcgg ggagaagaaa gatgcgacgt tagtcaagga
     1381 agcttttgca aagattccgt actcgttgaa taatgtacaa ctttttcaca cggatcgtgg
     1441 aaaagaattc gataaccaga cgattgacga aattttatgg gcctttaata tcacgcgttc
     1501 cttaagcaaa aaaggctgcc cctatgataa cgccgttgca gaatccacct ataaatctgt
     1561 caaagtagaa ttcgttcatc aataccgctt tgagacattg gctaatttgc gattagagct
     1621 atttgattac gtccattggt ggaataccat ccggctgcac ggcacgttag gctatgagac
     1681 accgatcagt tttcgacagc agagattggc gcagcgaatc cttgataatg agctcggatg
     1741 tgataccggt ggcgaggcac cttaattatt tgaatgagct tctgccttag agaatcagat
     1801 tcgaggaggc tcgttgtcaa gggcagtcgg aacaccagca gcaagcttac gcaatacctt
     1861 ataatttttg tcaaaaaaag tgttgccatt ccacatatat ctttaaacag ctattgccag
     1921 ataaaaataa atgatattat atatataaat acttatccga aaaggggaat gagagagtca
     1981 aggttcttgg aatgtcgaat agtcgagtag gtagattttc tatctacaac cttgttgttt
     2041 aaaaatgaaa aaaattggaa ttagagaagt tgcgaaagag gcaaatgttt ctcctacgac
     2101 tgtgtcgagg gtattgaatg atcgtggata tttgagtgag gaaacaaaaa gaaaagtttt
     2161 tgatgccatg aaaaaattag aatactatcc taatgaactg gcgagagcac tttttaaaaa
     2221 cgaaacatat accgtaggtt tgatttttcc tacaattact aatccatttc acgctgagtt
     2281 aattcaagat atcgagttcg agctttccag caggggatat aaagtgttgc tgtgcaatag
     2341 cttaaacaat ccaacaaaag aaaaagagta cttgacgatg ttgagaaaga atcaggttga
     2401 tggaattata gtaggaacgc acaataaaga tattgcggat tatgatattc cagggctacc
     2461 tattgtagcg attgatagaa gtcttggtga aaatacagtt acagtatctt gtgataatta
     2521 caggggtggg gaattagcta ctcaactact tgtaaatcat ggctgtcaga atatactgtg
     2581 tattcgggga gatagtaaga taaagctacc cgcgaatagt aggactttag catataaaga
     2641 tgttttgaat aaattcatgc tggaaccgtt agtcctagaa gtaccatttg taaaagaaat
     2701 caatgaaaaa aatgaaataa ttaagacata tctggaaact catccaaata ttgacggggt
     2761 ttttgcagga gatgatttat tggcttcttt ggcgattaac aacttaggaa gttcgggcaa
     2821 aagagttcca aaagaggtta aagtgattgg atttgatgga gctcaacaaa cattgattta
     2881 taatccagaa ttatcaacta ttcaacagcc tatagatcaa atatctaaag tagcagtaaa
     2941 aaaattattg aatatgatta agggcgaaaa ggagacggat gaactttcac ttccagtaaa
     3001 gctcataaaa aaaaatacag cataagttaa atcggcgaaa aattccttcg tcgatttaaa
     3061 aatattttta tgaaacgggt tgacatatga aacgggttga catatattag ttgtgaagat
     3121 aaaaaccttt tcactggagg aagaaaatgg agaaattatt aattaaaagc aataatcatc
     3181 aactttacta ccaacctaag gatgtttggg tcggagatgt tatgccttac gggaaagaag
     3241 gaaaattctt tatttatcat caacgtgata ctagaaatcc agttcctttt ggagaaccgt
     3301 ttggctgggc tttagcaact acgaaagatt ttgttcattt tgaagacttt ggggaatctc
     3361 ttttaagagg ggatgacaat gagagagacc agtttatcta cgctggttct gtatttgagg
     3421 caaatggaaa ggttcacgca ttttatacag ggtataatcg tgaattctta gcgcagggaa
     3481 agacatctca agtgttgctt catgctgaga gtgatgattt tgttcattgg gaaaaatcag
     3541 aaacagcatt ggagttagaa ccacaagaag ggtatgatat tcgaaactgg agagatccat
     3601 ttgtcgtttg ggatgatgaa aaagaagaat atttgctaat tttaggggcg cgaaagggtc
     3661 aggataaaca tctacaaacc ggacgtttag tggaatttac atcaaaagat ttaactaatt
     3721 gggaattcaa aggtgatttt tgggctccgg gattgtatac catgtttgaa atgccagaac
     3781 ttttcaagat tggtgactgg tggtatcttg tctattcaga atactcagat ggaaataaaa
     3841 tactctatcg aatgagtcaa agtttggaag gtccttggat taagcctaaa gatgaagctt
     3901 ttgatggacg ggcttattat gcaggtagaa cagcttatga tggaaaacgt cgagtcctat
     3961 ttggatgggt accaacaaaa gatcaagaaa atgataaaaa caattattta tggggaggta
     4021 ctttggttcc tcatgaagtc tatcaaaaag aggatttttc acttggggta aaacccatag
     4081 acgaagtgtg gaatgcattc ataaatttac atgatattgc cgatgtagag atagaaacag
     4141 cagacacaag agaagaagta acgcttgtca aaaatagtgg aactttgttt aatttagaaa
     4201 caacaatttc atttgatgag aatcttagtg acttttcact gagattatat aaaaaccttg
     4261 agacagatga atcttacgaa tatcgcttca atttagatga ggggcgtctt acatttgata
     4321 aaaacccttg ttacccttgg tttcaatata tggataaagg acttattaga cctatatctt
     4381 taaagcctaa ccaatcttat aaactaaaag tgattgttga tgacaatata atgacacttt
     4441 atattgatgg aatcgctctt aacgctcgtg tacatggttg ttttggtcaa gcaattacta
     4501 ttacagcaac aaatggatca atgactgcaa taaataccaa gttttcaaat gaaataaaga
     4561 aataataaac gtcaatcatg tggcaggtct gaaaatcaaa catgacctgt cacatttaaa
     4621 agggaggata aatgaatgga ctacaaaaaa attgccgaag atgtattgga aaacgttgga
     4681 ggaaaagaga atatctcaag cgctacacac tgcattactc gtttacgttt aattcttaaa
     4741 gacaaaagca aagcgaatat ggaaatgctt gaggaaatag aaggtgcaaa aggtgttctt
     4801 ttcaatagtg gacagttaca agtaattttc ggaacaggta taattgaaaa ggtttacaaa
     4861 gagttcgttg aattgacagg agctaaggaa gtatccgttt ctgaaatgaa agacgaagga
     4921 gtcaataatt caggaaatag acttcaacga gcttttaaag ttttctcgga tatctttatt
     4981 ccgatcattc cagcttttat tggggcagca atggttttag gaattcgttc gttgattacc
     5041 actcctggaa tgtttggaat ggatggctca ttcgctgatc acaatgtatt cctaaatgat
     5101 ttatccaaat ttctgggtat tattgcaacc acatttgatt atttaccagt tttagttatg
     5161 tattctgcaa caaaacgttt tggcggaaac cctattttag gattacttgt aggatttgtt
     5221 atgattcatc caggactagc caatcggaat gatttcgtca tgggaactat aaagcctgat
     5281 tattggcatt tatttggatt aagcattcca gcagttgcgt ttcaaggggg tgtttttcca
     5341 gcaattttga cttcttggtt tttagcaaaa gttgagaaaa ttacaaataa atattctcct
     5401 caagtacttg cttacatttt agtcccaact gtgacaattg ttcttgccaa ctttgcacta
     5461 tttttagtgt ttggtccatt ggggaatttt atcggtaatg ttttaggtgg aatcattgat
     5521 ttcctatata tgaagatggg tgcctttggt gcttttgttt ttgcaggcgc gttacaacca
     5581 ttagttgtaa caggaacaca acatgctatt caaggtattg aggcaaactt agtcgcaaca
     5641 actggattca attatatcca acctatttgg tcagtttcaa ttattgccca aggtggcggt
     5701 tgtattggta tgtatttact ctttaagaaa aaatctaaag atagagatat tgcgatttca
     5761 agctttattc caacactggt aggtattact gaaccggcta tctttgctgt taatttgaaa
     5821 tattcaatcg ttccttttgt ttgctcgttt attggtgctg gttttggtgg agcatttatg
     5881 aaaatttttg atgtaaaagc cattggacaa ggattgactg tattgccagg tctgactatt
     5941 gctaatccag ttcttcctta tatatttggt aatctcatag cattcatttt accaatcatt
     6001 tttattctcg cttatgataa atataaagtg tttatccctg aagctaaaca ccgtaaattt
     6061 gcagaaaaaa ctgactcaaa aaaattgcca gttcaagtaa agtgagatga gagcatgtta
     6121 aattttttta gaaaaaatga aataagcgtt gtagcacctg caaccggtgt ttttgaaaaa
     6181 ctggagaaat taagtgatcc agtcttttca aaaggaatga tgggacaagg atttgcaatt
     6241 gatgcaaaaa acgaaacgat tgtatctcca atcgatggtt ttgtttcaag tatttttcct
     6301 acaaaacatg ccttaggaat aaaaacaaaa tcaggaattg agatacttgt tcatgtagga
     6361 atagatacag ttgagctaaa tggtgaggga tttgatatta aagttcaaga aggacaaaaa
     6421 gttaaggctg gggatattct tatgaccatt aattttgtag tttttgaaaa gaatcacaag
     6481 actaaagatg tcattgttgt ttttccagac taccaagacg aaattttcat tccgaaactt
     6541 gaagaagtaa aacaaggaca agaattgttt gttcttggct aatgataagt aattaataat
     6601 agaatcgagg aaaatgtatg gtttttgggg ctattgaagc tggtggaaca aaatttgttt
     6661 gtgccattgg agataaagat ttgaacatat ttgaaaaagt cagctttccc acgactacac
     6721 ccaaagaaac aatgaattta gttttagatt tttttaatca tcataaagat gatttagaag
     6781 gtatcggagt gggatcgttc ggtcctattg atattcattg tgaatctaaa acgtatggct
     6841 tcattacatc aacgccaaaa cttgcttggc aggattttga ttttattgga agtttaaaaa
     6901 agcaacttaa tattccaact gcttggacga ctgatgtaaa tgcctcttgc tatggtgaat
     6961 atgtttctgg aaatggcaga ggcctttcaa gtgttgttta ctataccgta ggaacgggca
     7021 taggaggtgg cgctatccaa aacggaaact tcattgaagg ttttagtcat ccagaaatgg
     7081 gacatatgct tgtaaaacga cacaataaag acaacttttc tgggacatgt ccattccacg
     7141 gagactgttt agagggctta gctgctggtc cggcaatcga aagaagatca gggaaaaagg
     7201 gacaagaagt gactgaaaat gacgttactt gggaaataga agcttattac attgctcagt
     7261 gtgcatataa tacaacattg atgttatcac ctgatgtcat tatatttggt ggcggagtaa
     7321 tgaaacaaaa acacttaaaa aagaaagtac aagataagtt tattgagttg atcaacggat
     7381 atgtcgctat tcccgatgtt gacaattata ttatgacacc aaaattgtta gataattctg
     7441 gtattgtagg ttgtttagct ttagcaagag acgttaaaga aaaatcttaa tttggaaaaa
     7501 agatcgtctc gactatttgt ttggttgatg cgatcttttt ttcaattagt aattgaaggg
     7561 acgcctaaaa gtgaaaaaaa aaatgaaaga tgaagtatta gagtatatca aaaataattt
     7621 aaagtattac gatttttctg ctcaagatat tgcaatgaaa ttttgtatta aaagaaatgt
     7681 cgcttcccac tatttgaatc agttattttc agatggaaaa ttactaaaga atgattctgt
     7741 tagaccagta atgttcaagt ataatcaaca aaagccaaaa gactgctttt ctaagtttat
     7801 tggagcagac atatctttga aatccactat agataaatgc aaagctactg taatgtatcc
     7861 ccctaatggc ctcccattaa taataaaagg aaacagtggc gttggaaaaa gttttttagc
     7921 aagtttgatc tatcagtatg ctcttgatag aaaagtaatt cataatgacg caaaatttgt
     7981 tgttgtgaat tgtgcagatt atgcaaataa tccagagcta ttgtcagcag ttttatttgg
     8041 atataaaaaa ggagccttta ctggggctga aaaagatact gcagggatgc tatcttctgc
     8101 taatggagga tacctttttt tagatgaggt tcacaatcta agtgcagaaa accaagagaa
     8161 attgtttctt cttatggatt ctcaaaagta tcgtaaactt ggtgaaagtg tgcaatggga
     8221 gtatgcaaat gttcgcctta tcttagccac aacagaagat aaaaatacct ctttgttagc
     8281 aacttttaga agacgcatcc cagctgaaat cactctgcca gattacaata atagaagtac
     8341 atctgaaaaa attcaattat tatttgaatt tcttaaagat gaagcaatta cgattgattc
     8401 aaaaatattt tgttcgattg aactttttac tgatctttta tcgaaaacat ttgaaggaaa
     8461 tgttggagaa ttgaggaatg aaatcaagct tctttgtgca gaaggttatt taggaaataa
     8521 gagaaagtct tcaatttatt taggggacaa actagacagc ggtttttgga tagatccaga
     8581 aattagtata gatttaaaaa aaatgtttct taaatcactt tctgaaattg atttgattga
     8641 actttttcaa aatcacattt caattgaaga aagcatgagt caattaagag atagaatact
     8701 taaagagtgt gcaggttctt ctgagtataa ttatttagaa agtgacgaat ttatagcatt
     8761 aagaaactat gttgttaata aaatatcacc aattattgat tcgacaggtc tatgtttatt
     8821 agatgaattc ttggcggata tcagtctatt tataatgttt attgatgtaa ttgaagaaac
     8881 atcaagaaat tttttgctgt ctaagttcag aaaatttcgt atgaaaaatg ataaacaaaa
     8941 attattggca aatgagattt ggagtagttt agaagtcgaa gaaagtaaac aagaactttt
     9001 gttgaaatgg atactttttc ttgtaaagaa gttctatgtg aaaataccag aaacccattg
     9061 tctgatagtt atgcacggta aaataacagc ttctgcgatc gcaagggaaa caaataagtt
     9121 gctgaataca tatgtttatg aggctttcga tatgccaatt gaaggtgaga ctagtgactt
     9181 aattaaccta atcaacaatt tttgtaaaag tatagatacg acgaatggac tcattctgct
     9241 agttgatatg ggatcattgg aacaaatgta tgaaaaaatt gaatccaatg taataggtga
     9301 tttggtaatt ttaaacaatg tttctacagc tttagcgatt gaatgtggga ttcaagtttg
     9361 ccaaaaaaag ccaattagtc atttttacca gatggatttt gattcgtttc aagttaaagt
     9421 tcagtattat aaaggattat cacaaaagaa aaatgtaata gtatcttgtc tctctggtga
     9481 ggggatttct gaaaaagtta aggatatttt aaaacggtat ctcgataatc aagtggagat
     9541 tttgattttt gattttaacg cattaaagaa aatagcaaag gaaaaagata ctatgatttt
     9601 taagaataca atttgtgttc tttcaaccac tgaattttac attcaaggaa tcgattgttt
     9661 aaatttagaa aatctaatta atgggaatca aaccttagaa aaattaaata agtatatgga
     9721 ctcggatagc tgtgagttct gcttaaatga attagttaaa ctctttacta ttgaaggcgc
     9781 tagtactaag cttcgctttt tagattccaa aaaagtattc aatgagatag aaaaagtact
     9841 atatttgtat gaaaaatatt acgatgtcaa aatcccaagt tttctgagaa ttaatctatt
     9901 tctacatctt tctggaatga ttgaacgaat tatgattggt gatggaataa caaatgaaca
     9961 tttgaaagag ggagcgcaac tatttgatac ttttttaaat gtatcaaaag attttttttc
    10021 agaaatcaaa gatatttact gtattgaaat acccaaagga gagtatgagt taatctatac
    10081 aatttttgag cagactattt tctaaatagc atactagcgt ctaaaaaaat gaaagggtgt
    10141 ccaaagtata tttagacgct catttaatta atatttactg tattttcccg ctttattgga
    10201 taatgttagt tggcacagta attgcttata taaaagtata agaagaaagg agagaacaaa
    10261 atggcgtttt ggcaaacatt tttagttgtg ttgtatggat ttttcattaa ctgggataag
    10321 gaagggccca aaatcggaac ctcccaacct gtcgttgcag gtttcttgac cggacttatt
    10381 atgggagata ttcaaactgg actttatatc ggtgggactt tgcaattaat gacactaggt
    10441 attactagtt ttggtggtgc atcagtaccg gattatcaaa cagcagcatt gatcggtact
    10501 tatttagcaa tctcaactaa ccaggatgct tctataggaa taactttagc aataccggta
    10561 gcaatgttaa ttgtacaact agatgttcta aagtggtcaa caaacatttt ttttcaacaa
    10621 aaggcagaaa aattcgcaca aaaagaagat tatcaaaaat tagagtggat gcaatatttg
    10681 gcatcaatta atacaagctt tgtatctgga attcctgttt tgttaactgt gatattaggc
    10741 ccaacgttag tgggaagtgc aatcaattat attcctgaat gggttaccgg tggtttaaag
    10801 gtagcagggg gactattacc tgctgttggg ataggaatgc tacttaaata tttacctaca
    10861 aaagattact ttgcctattt gttagttggt ttcgttttgg cagtttattt ccaaatacca
    10921 attttaggga tttcgttact tggcgctgca atcgcactaa tcgtatataa aaatagatca
    10981 aataatactc aagtagcacc agctgcaaca ggaggttttg acgaagatga ataagaaact
    11041 gacaaaaatc gacaaacaag taattttccg gtggattctc aatggtgcaa cttcattaaa
    11101 ttatgaaaaa atggaagggt tgggttatgc gtattctatg cttcctttta tcaaggacac
    11161 atataaagat gatcctgaag gaatgaaaca atccgtttta gctcacttac aattttttaa
    11221 cactacgccg tatacagcgc cttacattat tggtatgaat gttggaattg aaaaagaaga
    11281 aaaaaataag tctttggaag cagtaagtgc tctgaaaaca ggtctaatgg gtcctttagc
    11341 gggaattgga gatagtgtat ttgtagttat tccatggact atttttggag caattgcagc
    11401 taatatggca ttggaaggta gttttgttgg aattattttg tggatactgg catcaatttt
    11461 attaaggatt actgcttatc ctttatacct tgctgggaaa gaatcgggtg cgagcctact
    11521 aactcaaatt gaaacaaaat taaagtctat tactgatgca gtatcaattt tagggttaat
    11581 ggtggtcggt gcactcattg ccacagttgt aaaagtgaat gttggctttg aatttaaaca
    11641 aggcgactta acaatgaccg gaaatgaaat ctttgatcaa gttatgcctg gtttattacc
    11701 tgcatgtgta gtagcaattg tgtattattt gttaggacga aaggttaaac cagtttatgt
    11761 aattctatta atcttagtag tttctattgc gttaagagcg ttaaatattt tgaagtaggt
    11821 gttgaatatg gatgaaaaaa tgaaaggtat tatacatgtt cgtattgatg atcgaatgat
    11881 tcatggacaa gtagcaacac aatggacaaa tcaattgagt gctaacagaa ttatggttat
    11941 taataatgaa gtagccaatg atgacgtaaa aaaagctgta gttcgcctcg ctgcaccacc
    12001 taatgttaga acttcaataa ttacaaggga agtagcagtt aaaaatattc tttctggtaa
    12061 atatgaagga caaaaagttc ttattgttgc agtttctcca atcgatgtta aatttttgat
    12121 tgataacggt ttaccaataa caagtgtaaa cgtaggaaat ttatcaagaa ggaatggaac
    12181 agaacgcatc cgtccgacga taaatattac tgaagaagag agagaggctt ttaaagaact
    12241 aattagtgat ggagttgaag tgacagtgat acaaactcct agagattcaa aagaattttt
    12301 aaaggatttt atttattaag aacagaggat gagtggatgg agtttataat tacatctcat
    12361 ggaaatattg ccattggatt tgtagataca ttaaaaatgt tttttggcga taatttaaat
    12421 tgtcatgtgg taacacttgg agatgcaggc attgaagaat tccgtgaaaa cattgaaaaa
    12481 atagtttctc aagtaaaaaa tcaagaagtt atggtatttg ctgacttgat tggtggaaca
    12541 cctttcaacg aatttgctaa aagaagtgaa gtatttcaaa atgggtttca acttcttggt
    12601 ggcatgaatt taggaatttt gattgaggct gtcaatttga gattacaagg aaaaaaaagc
    12661 gatgatatca tcgaaaatct aagaaatatg aatacaattg cgtgtcttgc agatatgcaa
    12721 agacatacta acgaagagga tgagtgataa atgcaaaaaa ttttttacaa gcctaaagat
    12781 ttttggtttg gggattgcat gcctttttat aaagatggaa ctttttatct atttcatcaa
    12841 cgagatacga gaaatcctaa acctttaact gatccttttg ggtggtcact agttacaacc
    12901 actgattttg tgaaatttga agaatacggg gaagtaattc atcgtggtgg caacgatgac
    12961 attgatcaat acatttacgc tggaagtatt gtccaaggaa tcaatgagta tttagctttg
    13021 tatactggac ataatcgcaa agctaagttg gatggaaaaa cttcagaagt gttaatgatt
    13081 gcaagttcta aagatttaat tcattggaaa aaggatggcg aagcagcaaa acttgttcct
    13141 caagaaggat atgataagaa tgattggcgc gatccttttg caatttttga tcaagatcat
    13201 ggaaaatacg ttttgatact aggagctaga aaaccagcaa gtaagaaaaa gccgacgggg
    13261 agattggtct attttgaatc cgaagattta aaagattgga atttcaaagg agactactgg
    13321 gctaataatg aatttaacat gattgaaatg cctgatatat ttgaaattga tggaacgtgg
    13381 tatttacttt tttcagagta ttgtgaagat aaaaagacta aatatcgagt cggagcagga
    13441 ttattttcag attggaaatc accagtagac gaagctttcg acggaaaagc ctattatgca
    13501 gcaagaacag tcggaactaa tgaaactggc cgttttctaa ctggctgggt ggcaacaaaa
    13561 gaaggggaga acgatcaaaa taactgggat tggggcggtg cacttgttcc acatcaaatt
    13621 attcaaaaga cagacaaaac tcttggtgtt aaactgcctg aaaaaataat tgagaatttt
    13681 aaagaaatca ctccattttc aagttttaag ttagagaaag aatttggaaa aaaagaaaga
    13741 gtaattgttc ctaaaatcga agattatttt ctatttgaaa caactttgac tattacatcg
    13801 ttaacaaagg agtttgcttt aaaattattt gtagatagtg atactcaaga aggatacgaa
    13861 tttcgtattt cattgattaa taatcagctt actttcggaa aaactccgaa ctatccttgg
    13921 cctcaaatgt ttgataaggg gctgcaacga ccactatttt tggaaatagg caaagaatat
    13981 gaactaaagt taattgttga tgatacgata gctgttttat atattgataa tgttgcttta
    14041 agtactagaa tgtatgataa agtcggaaca gcattaagta tgactgttac ggagggagca
    14101 atcaaggtag ataaactaga ctattcaata aattatgaat gtggatttta aaacaattga
    14161 agggatgatg taagtgaaat taaatggttt acaacatatt ggaattccaa caaaagattt
    14221 tggagcttca aaaatctttt atgaatcctt agggtttagt ctgattaatg aagaaatgaa
    14281 tgtgggaagt aaagtcggct ttttcgagtt gaatggaacg ataatagaaa tatgggaaga
    14341 taaaactact gagtgtagag gagccattga tcatctagca ttagatacgg atgacataga
    14401 tgaggtattc aa
//
LOCUS       DACDRK010000066        14107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDRK010000066 DACDRK010000000
VERSION     DACDRK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.066"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IWJ77_002099"
     CDS             66..767
                     /locus_tag="IWJ77_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9453684.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IWJ77_002100"
     CDS             764..1885
                     /locus_tag="IWJ77_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9453685.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IWJ77_002101"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IWJ77_002101"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9453686.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IWJ77_002102"
     CDS             3264..4172
                     /locus_tag="IWJ77_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9453687.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IWJ77_002103"
     CDS             4207..4479
                     /locus_tag="IWJ77_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9453688.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IWJ77_002104"
     CDS             4490..5536
                     /locus_tag="IWJ77_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9453689.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IWJ77_002105"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IWJ77_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9453690.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IWJ77_002106"
     CDS             complement(8547..9218)
                     /locus_tag="IWJ77_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453691.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IWJ77_002107"
     CDS             complement(9211..9705)
                     /locus_tag="IWJ77_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9453692.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IWJ77_002108"
     CDS             complement(9725..10945)
                     /locus_tag="IWJ77_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453693.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IWJ77_002109"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IWJ77_002109"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9453694.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14103)
                     /locus_tag="IWJ77_002110"
     CDS             complement(13021..14103)
                     /locus_tag="IWJ77_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9453695.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DACDRK010000067        13679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDRK010000067 DACDRK010000000
VERSION     DACDRK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.067"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IWJ77_002111"
     CDS             complement(<1..902)
                     /locus_tag="IWJ77_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9453696.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IWJ77_002112"
     CDS             1185..2153
                     /locus_tag="IWJ77_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9453697.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IWJ77_002113"
     CDS             2266..2544
                     /locus_tag="IWJ77_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9453698.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IWJ77_002114"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IWJ77_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9453699.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IWJ77_002115"
     CDS             3010..3756
                     /locus_tag="IWJ77_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9453700.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IWJ77_002116"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IWJ77_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9453701.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IWJ77_002117"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IWJ77_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9453702.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IWJ77_002118"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IWJ77_002118"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9453703.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IWJ77_002119"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IWJ77_002119"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9453704.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IWJ77_002120"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IWJ77_002120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9453705.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IWJ77_002121"
     CDS             8493..9035
                     /locus_tag="IWJ77_002121"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9453706.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IWJ77_002122"
     CDS             9058..10614
                     /locus_tag="IWJ77_002122"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9453707.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IWJ77_002123"
     CDS             10628..11530
                     /locus_tag="IWJ77_002123"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9453708.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IWJ77_002124"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IWJ77_002124"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9453709.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IWJ77_002125"
     CDS             12973..13395
                     /locus_tag="IWJ77_002125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9453710.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4521 a         2448 c         2980 g         3730 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca
     9901 ggtcgtgaag cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgcctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaagta attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgctgctc agccgtgctg tgaagaaaac tggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg ttccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattacaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggggaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca
    13561 gaggttgtaa aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttattt
//
LOCUS       DACDRK010000068        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDRK010000068 DACDRK010000000
VERSION     DACDRK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.068"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWJ77_002126"
     CDS             complement(<1..914)
                     /locus_tag="IWJ77_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9453711.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWJ77_002127"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWJ77_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9453712.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWJ77_002128"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWJ77_002128"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9453713.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWJ77_002129"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWJ77_002129"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9453714.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWJ77_002130"
     CDS             4440..5321
                     /locus_tag="IWJ77_002130"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9453715.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWJ77_002131"
     CDS             5318..6034
                     /locus_tag="IWJ77_002131"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9453716.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWJ77_002132"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWJ77_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9453717.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWJ77_002133"
     CDS             6751..7752
                     /locus_tag="IWJ77_002133"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9453718.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWJ77_002134"
     CDS             7749..9110
                     /locus_tag="IWJ77_002134"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9453719.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWJ77_002135"
     CDS             9070..10221
                     /locus_tag="IWJ77_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9453720.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWJ77_002136"
     CDS             10196..11365
                     /locus_tag="IWJ77_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9453721.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWJ77_002137"
     CDS             11540..12919
                     /locus_tag="IWJ77_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9453722.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWJ77_002138"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWJ77_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDRK010000069        13324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDRK010000069 DACDRK010000000
VERSION     DACDRK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.069"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            13..144
                     /locus_tag="IWJ77_002139"
     CDS             13..144
                     /locus_tag="IWJ77_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9453723.1"
                     /translation="MATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRKRVSR"
     gene            complement(206..2017)
                     /locus_tag="IWJ77_002140"
     CDS             complement(206..2017)
                     /locus_tag="IWJ77_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9453724.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2014..2379)
                     /locus_tag="IWJ77_002141"
     CDS             complement(2014..2379)
                     /locus_tag="IWJ77_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9453725.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2542..2937)
                     /locus_tag="IWJ77_002142"
     CDS             complement(2542..2937)
                     /locus_tag="IWJ77_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9453726.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2955..3917)
                     /locus_tag="IWJ77_002143"
     CDS             complement(2955..3917)
                     /locus_tag="IWJ77_002143"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9453727.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3917..4129)
                     /locus_tag="IWJ77_002144"
     CDS             complement(3917..4129)
                     /locus_tag="IWJ77_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9453728.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4150..4848)
                     /locus_tag="IWJ77_002145"
     CDS             complement(4150..4848)
                     /locus_tag="IWJ77_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9453729.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4868..5410)
                     /locus_tag="IWJ77_002146"
     CDS             complement(4868..5410)
                     /locus_tag="IWJ77_002146"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9453730.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5542..6546)
                     /locus_tag="IWJ77_002147"
     CDS             complement(5542..6546)
                     /locus_tag="IWJ77_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9453731.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6543..7532)
                     /locus_tag="IWJ77_002148"
     CDS             complement(6543..7532)
                     /locus_tag="IWJ77_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9453732.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7529..8335)
                     /locus_tag="IWJ77_002149"
     CDS             complement(7529..8335)
                     /locus_tag="IWJ77_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453733.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8501..9457
                     /locus_tag="IWJ77_002150"
     CDS             8501..9457
                     /locus_tag="IWJ77_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9453734.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9534..10052)
                     /locus_tag="IWJ77_002151"
     CDS             complement(9534..10052)
                     /locus_tag="IWJ77_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9453735.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10140..10289)
                     /gene="rpmG"
                     /locus_tag="IWJ77_002152"
     CDS             complement(10140..10289)
                     /gene="rpmG"
                     /locus_tag="IWJ77_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9453736.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10517..10963
                     /locus_tag="IWJ77_002153"
     CDS             10517..10963
                     /locus_tag="IWJ77_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9453737.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11158..13053)
                     /gene="asnB"
                     /locus_tag="IWJ77_002154"
     CDS             complement(11158..13053)
                     /gene="asnB"
                     /locus_tag="IWJ77_002154"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9453738.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4146 a         2830 c         2272 g         4076 t
ORIGIN      
        1 ttagtaggaa gtctggcaac agccgctgcc gttgctggtt tggttgcttc tgtgaaaaaa
       61 acagtgatcg atccaattga cgaaaaagaa gcaatgatcg aagaaaaccg taaaaaagca
      121 atgcgtaaac gcgtttctcg ttaataatta tttaaaaata cgcatgaaga ctttctaaaa
      181 aacagataag tcatcatgcg tatttttatt tgtcagtcaa tataagttgt gctattttat
      241 ctgcctgtct cagattccat ggatctgtat aaggtctagc tactgctgca gttagccaat
      301 ccacaccttc aacaggtatt ccatagcaat aaaagtcaga ctggatttcc cctgaagcat
      361 caatcacgcg attcgtacct cgctcaacat ctatagcttc tgaatgataa cttcctgtat
      421 tatctgaaaa tgaaaaaggg cgaacaagtt tgttttcctt caaatttttt atgacaggat
      481 tacttgaacg agaaaagctt gtttgtggca atcgagcttc aatcaaatag cgactgctga
      541 tttctttatc ttgtagagaa ataacatagc ggttgtgctt ttgctgaata tcaaatggcg
      601 gttcgatcaa atgaaaaata cccgcatcta tcagtgctgc taattctttc gtccgaatga
      661 ttggcggacc gatcgttaaa aagctattta gatgagtaaa ccatccccat agtatttctt
      721 tgtattcctt agcagaaaaa acgtgccatt caatagcctg ccggattgga tcacgccagt
      781 cttttaatgc atcaaatgtt gaagcaaatg ctcctgtgca attgcctttt tccgcttctt
      841 ttatctgccc ttcaataaat attcggctag cttctgaaaa gggctcattc tgataggcaa
      901 gaggagtttc taaatacgac caatcccatc tataagggat tagtgctgga tgctttccga
      961 gacactcctc tgaagagagg gagagaaaat catgctcaaa atttttcttt ggaatcggca
     1021 agtgatattc ttcgatgatt ttcttgtaat aaaacaactc aacgtctttt ttcagatagt
     1081 caaaaaatgt ttcgccagtc aaaaatcccg actgatgcca ttgctccagg ttttcttttg
     1141 ttagtaaccg tggccatgcc attgctccgc cttgcttttg atttcttcct cgagggtaat
     1201 aagggagccc ccttcttgat ccactgtata caatcggttc cttgcctgat ggatgataga
     1261 ccagttttcc cttttcgttc tcgaagcgcc ctcctcttga tactgtaaac aatccgacat
     1321 aatcaaaaaa acttaagcct agacctctta aaaagacatt ttctttaggc ggtatcttcg
     1381 aaagatctac atctgcagga ttagaaggaa gctgatacag taaaacattt ttctttgcgt
     1441 agtctaccca cactctttct tcttctgtct catcattttc ccagtgaccg cttgctaaaa
     1501 caagctgatc gatagaatag ctttgctgat ctgtctgtaa aagataacca tcttttcttt
     1561 tgcttacttt ttttactaaa gaataaacaa actgaataga agctggaaat tcttcttgca
     1621 actttgagaa aaaccagcgt tgatagagtc cgtataaaca tcgagtagat tgctgatctt
     1681 tttccagacg attgatttct tctataatta gttgcttatt tttggttacc gtctgttcga
     1741 tataatctgt agcaggtcct ttactccatt gaaacaagtt gggtccctgt gttaccaatc
     1801 ctccactgga caatgtttca tctgtaaaaa gcgtcacttg ttgagaaaca gaattcatta
     1861 ataactccgg tggctgattc aaccgccaaa ctcgacctcc aggaccattg ggatcgaaaa
     1921 gaaaaagttc aagagaatgt ctgtcctgat tattatggag aatcctttct gctacactta
     1981 atcctctagg tccggcacca atgatcccta ttttcatttg catgcctcca tttcgataaa
     2041 ttttttcaat aaaacagcat ggttctgatg ttcatttttt gcaccataaa caaaagtaat
     2101 gactggatgt tctttcataa gttgactgag tttaaaaata gcttgcttct tttcttcttc
     2161 gaaaatcaat tcttgctggt atttctttgc aaatgaagca aatcgttctg gtacatggtc
     2221 aaaagttttg cgtgcttcat tactaggtgc aatttctttc aaccacagat ctaactgcgc
     2281 tttttctttt gaaattcctc gtggccagaa tctatccacg agtattctat agccatcctc
     2341 tttttcagca tcaaggtaaa cacgttttaa tttgatcata gttgtttcct tctttctacc
     2401 ttttcgctta gcttatatat gtagataata gcacaaaata aaaaaaatga acggcacaac
     2461 tatccattgc gccgttcatg tgatcttggt caaaatctaa tgattgattc ggtttttaaa
     2521 gctgtcatac aatcatctgt tttatacttt tttacttttg atttttccag accatccgtc
     2581 atatccaccc ttaagtaaat aaatctcttg ataacctgct ttacgtaatt tatttgctgt
     2641 acggatgctc aaacttttct tttgatcgta aatatagaca ggctggtctt ttcgaataga
     2701 tcctaatgaa tctttcaaaa cagtgtaagg gatgtttctt gcacctaaaa tatgcccagc
     2761 atcaaacaca tctttttcgc gaacatcgat tacttgtgct tttctcattc cttctcgaaa
     2821 ttcttcttct gtcaaagtcg ttgctgaacg tttggccatt actcgtagat agacttccca
     2881 aaggatgatt gcaagcacaa tcaatagcaa aatgatatta attacccata aaaccatgta
     2941 atgcctccgt ttttttaagc aaatttcaag gctaatgagg cagcaccaat gacaccagct
     3001 tcattaccaa gctctgctaa tttgattttt gttgattctc ttacttgcgg gaaagtaaat
     3061 tctttaaaat atttctctac acggctacgt aaaaattctc ctgctgccga tactcctcca
     3121 cctaacacga tacttgacgg atttaatgta ttccccaagt ttccgcaagc taaacctaag
     3181 tagaagcata cgcgatcaac aaccatcaat gcaaacggat cgtcttcttg agccatttca
     3241 aagatatctt tacttgttac ttcttctccg ttgtctagca ttgctttcag ttttgaatcc
     3301 cctgcgtatt cttctgccaa gaaacgacct aggcgtacca cacctgtagc gctagataca
     3361 gtttctaaac atccgcgttt accgcaagta cattcgaacc cttcaggatc cacagtgata
     3421 tgtccgattt ctcctccagc tccgctcacg ccgtgaatca aatttccgcc agcaacgata
     3481 cctccgccaa cacctgttcc tagagtaacg aatacaacgt ctggatcgtt ttctccagca
     3541 cctttccatc gttcacctaa agcagcaaca ttcgcgtcat tatcgatagc gaatttgatt
     3601 cctgttcctg cctcgatcag ttttttcaca aattgaactt ctgtccagtt taagttatac
     3661 gcaccaataa ccgttccagc ttcgctgtcc acgcttcctg gggtacccat gccgattccg
     3721 ataaagtctt cggctttcat attatataaa ttcaaacgat gattgattga ttcaatgatt
     3781 tcaggaacga tatgcttacc atcatccaaa atattggtat cgatgctcca tttttgctga
     3841 atctctcctt ctggtgttaa aattgcaaat tttgctgttg ttccacctaa atcaatccca
     3901 ataatcttct tgtccattac aaatttcctt tctttcggct ttcttcaatt cggtgctctc
     3961 tttttaatat gcgccacgct gtcatgtatg tatcatgatc aatcaaacgg ctatcataca
     4021 gattacgcaa ttcgatagtc attaattcaa tgtcatagat gcgtgcaccc acatagataa
     4081 agatgccaaa ttttttgaac agctgctgta catcatataa gctctccatc tttaagcact
     4141 tccttcatat tatacaagta aaccatattt ttttaaaccc agtaaaatac atattacaaa
     4201 aagaaataca aaaactagtg tagaaagaat cctttggtgt atcgaatagc ttgtctgttg
     4261 tttcggtaga cccaaaacat tccctaacac taatccgcca attagcccac cgacatgtcc
     4321 ccaaacatct acagaggtat ccagaagacc aaaaacaaat gtcaacacaa taaataaaag
     4381 aaaatgtctg actagctgct ggatcgctgg gttatgttta aaatgaaacc ctaagataaa
     4441 caacgcaccg aacaaaccga atatcgaagt acttgcacca ccagacagaa cattcgcttc
     4501 gttaaaagca aagcttgctg tatttcccat tatcccgctg aacatataaa tcaagaaaaa
     4561 acgccaatgg ccatagattg cctcgatttg ctgccccata aaatacagta tcagtgagtt
     4621 cattacgaaa tgcatcagtc caaaatggat gaagattggt gtcactaaac gccaccattc
     4681 attgaagtga acaagataag gaccatacat tccggcataa taagggatct caagtcgcgg
     4741 aaataattcc atcatgagga agaccgctgt ctgtatccct aaaaaagcat aggtccataa
     4801 cggtcgtctt aaccatgcac gcaattttat ttgctgttga taattcatga aaacctccca
     4861 tttcatttta gtcaataaac aattgttgga tcttctgatc aaagctctcc ggttgccatt
     4921 gttcattcaa ttgttcacga aaaacaaggc tacatgtttt tttatgaaaa ttatttaaat
     4981 accggtcgta ataaccaccg ccaaaaccga ttcgataacc ggctctattg tagataatac
     5041 ctggtacaat gaggagatcg atcatctccg gcaaatagag cttctctgat tgtggctcaa
     5101 gaacaccaaa actcgaacgt acaagaacgg actgttcatc catttcataa aacgccatct
     5161 gacgtttcgg caaagtttta ggtacaacta ttttcttttg ttctttttct gctcgttgga
     5221 taaccggctg tgtatccagt tcaaattcat tcgataaagt cgtccctatc acttttgctg
     5281 acttccagta ctcgctggcg aatagctgtt tatagattcg ctgttctttt tcctttttct
     5341 tgccactttt ggccagattt ttcagctttt cgattccttt ccggcgtaat cttactttca
     5401 attggtccaa aaactcaccc ttccttctct cataaaatgg tataggaaaa aaagaaaaat
     5461 agcaatcgaa ctttcaaata aatgcataaa tagaataaaa agaagaccta gaaatgattg
     5521 gccttccttt tttaatgaat ttcaactgtt cagcgcgtgt ttttgtctga taaaaagtgc
     5581 atacagaaaa tatggcgcac caaaaagggc aatcaaaatt cctgtaggta cttccccact
     5641 tggtagaatc actctagcaa ccatatctgc actaagcaga aaaatggaac cgaacaaacc
     5701 gctcagaaca aaggtccagt tattttcttt acgtacgact agcttcgcag catgaggagc
     5761 tagtagtcct acaaaagata aactacctgt caccgctaca ctaccgcttg ccaaagcaac
     5821 agctaacaac aaaaggattt tttgactttt agccacgtta aggcctaatc cttgcgcgcg
     5881 ttcttgacca aatgatagca atacaatcct ctgtgcataa agtcctccta gcggaattaa
     5941 gataaccgcc catggaagca atgcccaaac ataaggccag ctcgcacccc agattgatcc
     6001 cgctaaccat gcattcacaa atccataatt ttccttagat aattggatcg tgcatagcaa
     6061 tgttaatgca gaaaggccgg cattaacggc aataccggac aataagattc gattcatacc
     6121 caaaaagcca tttcgatcat aggccgcaag atagacacag caagcagcta aaacaccacc
     6181 tagaaaggct acaactagca atagaaacgg cagcgaatag tgcgaaaaga aacctaaata
     6241 aagcatcact aaaaatcctg ctccggcatt aatgcccaat atactagaat ctgccaattc
     6301 atttcttgtt actccttgca agatataacc agaaacgcca agacaagctc ctgctaataa
     6361 actgatcagt aatcgaggaa ctcgaaattc tctaacaatc agcgccaaag aagatgattc
     6421 tttccctatg aacgtggcaa aaagctgatc caggtaaata aaaggcgtac caatcattaa
     6481 actacctata gacaggatga caagtacaaa aagcaagaaa tagaacgtcc aatatatttt
     6541 tttcaccgtt gttccctcct tacttgtatc agtaaaaacg gaacgccaat caatgcaatg
     6601 actaatccaa aaggtgtttc aaaaggggga ttgatcatcc tcgctgtaaa atccgccaac
     6661 ataaccaatc caccgccaac taaaaaagag acaggtagaa cataacgata gtccgttcca
     6721 accattccgc gaacaactgg cggaaccaac agcccaataa atgaaacagg tccgactaac
     6781 gaaacggcaa tggctgctaa cactgcaact agacagaaaa caatcaaccg cgttcgttta
     6841 ggatgacttc caagagctaa tgcatgtgtt tcactcaaat gaatcaaaga tattgctttt
     6901 cctaatgaaa aagaaaccaa aacagcaagt atatataaag gaaacaatac tgctaattgt
     6961 ttccatgtga tattggcagc tccgcctaca aaccaaaaag caagatcctg ctgcagatta
     7021 aagaattggg taagtgcttg gctgatagca gtaaaaaaag aactaatagc tacgccagct
     7081 aaaatcagct gtaaaggatt catgcccatt ttcgaaaact gcaaaaaacc aaatacgagc
     7141 aaagttacag caaaagaacc tgccaatgac attagaaaca cagaaaaatc agaagcttta
     7201 ggccaaaaag caaaactaag ggccattgcc aacgaagcac ccgcgttgat tcctagtaag
     7261 cctgaatctg ctaaaggatt tcttgtcacc ccttgcatca atgcaccgct caatgcaaaa
     7321 caactcccta caagaaatgc tcccgccaca cgaggcaaac gaatatttcg tatgatttgg
     7381 tgactttgtt ttgatctatc aaaatcaaac aacgcctgcc aaaaatctgc tttttccaca
     7441 gtcgctgcgc catttgaaag agcaacaaaa aaaataatca aaagaacaaa acagaccaaa
     7501 agcaggagtc tatttttctt catagtcctc ataattgatc ctcctgctct tttggcaaca
     7561 aatcataggt tagaaaaact ggtttatttg ttcttggaag cgtgacaatc gttgcatcaa
     7621 tctcaaatat ttttttcata cactcttttg tgatcacttc ttcaggtgta ccttctgtga
     7681 tcaacttacc gtttttcatt gctaataaat aatctgaaaa acgtgaagca tgattaatgt
     7741 catgaatcga catcaagatc gttgtttgtt tcatttgatt gatttcagtc aataactgta
     7801 ggatctctaa ttggtgtgcc ggatctaaaa atgttgtcgg ttcatccaag atcaagatat
     7861 ctgtttcctg agcaagcgcc atagcgatcc agacacgctg ggtttgtcca ccagacagcg
     7921 ttgcgatatc ccgcatacga aatccagcta gacctgtagc atccaacgcc cactcaattg
     7981 cttcatgatc ttgcgtgtcc atgccactga acagtttttg ataagggtat cgtccgtaag
     8041 atactacttc ttctactgtc atacctgtag ggtgttcact agtttgtgct aataatgcaa
     8101 ttttttttgc aagttctttt gaactgtatg tagagatatc agagttattg acaatgatct
     8161 ttcctgattt cacaggtagt atacgggtaa aacttttcaa taaagtagat tttccacttc
     8221 catttggccc aatcaaacta gtgattttcc cttcaggaat cgtaacagac atgttttcaa
     8281 tgattttttt gttttcgtag ccggctgcaa tgtcagctgc tcttaatctc gtcatttcac
     8341 ccatcctttc gctgaaaatc attctcaatt atgacgctgt ttcaacattt cgtcaagcgc
     8401 tactttcata tctatagcca aatgaagcgg ttgacagcgt tcgaaatatc aggaataata
     8461 accaatgaga aagattctca attatgttag gaggaaagaa atgaaattaa aaacaaaagt
     8521 attcacatta tctagcatat tgctgctggc tggagtactg agcgcttgct ccccctcttc
     8581 agataaagaa acagctacaa ctgccaattc gacgacttca tccaatagta ctcatacagt
     8641 cacagataca cttggacata aagtagagat tccaaacgcg cccaaacgga tcatcggcag
     8701 ttacttagaa gactatctga ttgcactagg ggaaaaacct atcgcccagt ggactgttgg
     8761 cagtggttcc attcaagatt atctgcaaga tgatttaaag gatgtcccaa cgatttctta
     8821 tgaccttcct tatgagaaag tgttaagctt tgagcctgat ttgctgttga tttcttcttc
     8881 cgctactgtc gaaggcggga aatacgaaca atatagcaaa atcgcgccaa cttatgttgt
     8941 aaaaaacgga acagatgtca catgggaaga acaactaaaa gatgtcggca aagctttggc
     9001 aaaagaaaaa gaggcagaaa aaatcatttc cgattatcaa aaatccatca agaaaactcg
     9061 agaagaacta gcagataaaa tcgagaacaa gacagctgca gtgttgtggg tgaccaacaa
     9121 ctcagccttt atggtcagca aaaatcggtc aagtggaaga atcgtatacg acgatctgga
     9181 atttggtgtt cctgatctag tggaagaggt ctccaaaaaa gccacttctg actggtctgc
     9241 tgtttcctct gaaaagctag ctgaattaga tgctgactac atcatccttg tgaacagtga
     9301 caaagatgct gccatgttca atgaaccaag ctggaagaat ctaaaagcag tgacagacaa
     9361 tcatgtactg caattcggac ctgaatctag ttggctatat aacggaccta ttgccagtca
     9421 ggaaatggtc aatgatatca aagaaaaact aaattgattt taactaaaaa aagcatcttc
     9481 acgattagtc gtgagatgct ttttttctta ttaacaaact agacgcatag attttattct
     9541 tcttttgaag caaccctatt ttttactctt tcccattttt gaaaacgatg ggcatataga
     9601 ttttcttgat ccgttatccc ctcatactcc tctataaggg cgaagtctga aaaatcaata
     9661 ttcccaatat acgtatcgcc ttcaaacgtg ctatcgatca atgttcgcca gatccgacca
     9721 gtgtaaggaa gaagttcttg aaaaatccga ctaccacccg aaacatagat atcttctgag
     9781 atcgtttgac tatacgacaa aagttcatcg atcgaatgca ttacttccgc attttctttc
     9841 gtgcgaaaat ttggttgcgt tgtcaacacg atgatatggc gataaggaag tgaaagatcc
     9901 cccattcctt cataggtctt tcttcccatc actaaaattc gatccattgt atgttcgcga
     9961 aagaaacgca tatcatttgg caatcgccat ggtaacaaac catttttgcc gatcagacca
    10021 tttttatctt gtgcccacat ggaaataaac atattatccg ctccttatga ttttagatgt
    10081 tttgagataa aaaaagtctc aattctttga ccagcaagga attgagaact ttaaaaacat
    10141 tattttgttt cacggtgcaa agtaactttg cgttcgcgtg ggcaatattt ttgtttttcc
    10201 aaacgatcag gattgttacg tttgtttttg cttgttagat agttacgttc tttacaagaa
    10261 gtacactcta aagtgatgtt tacgcgcatg tttttccctc ccatttttac aagaatcctc
    10321 taagatgact ctcagacttg attatcttac catgattttt tctagattgc tagtgttttt
    10381 cacgattttg ttaagtaaaa ctacttgacg ataaatacaa cggttttact tctcaaacaa
    10441 aatctttttc tttttgtata aataccctta cacttgttta tatagattat gttttttaat
    10501 aaagggggat actaatatgc catggaacat gaaggacttt cctgcatcaa tgaaaaactt
    10561 agataaattg acaagaaaaa aagcaatcga catcgcaaat gcattattag atgaagggta
    10621 ccctgataac cgtgcgatcc caattgccat tgatcaagca aaaaaatggt atgaagatgc
    10681 tagtgaatca gaaaaacgaa ctttcgaaaa agaaaaaaat ccaagcaaga cggatgaaca
    10741 cgatacgaat cctcgtgctg gaaaactgct tgattcagat gtgatcgtag aatatgagga
    10801 agaacaatgg atcgtgaaat ctaaaggggc aaaaaaggca agcaaccatt ttgatacaaa
    10861 aaaagaagca atcgaaaaag gaaagcaagt agcacaaaac aaagaatcaa ctttgttcat
    10921 ttataaaaaa gatgggacaa aagaaaaaga aatcgattat tgaacatatg taaaggggtt
    10981 gtgacgaaaa tcgtgtcaca acccccttat gttcgaaaca atggtgttcc tctgccaact
    11041 ccgaaccata agtcataata tctataaaac gggaagagct gttttttgaa attgtttctt
    11101 atcactcagt gttaaacggt aggagaaaac ctctttttta ttgatatttg attttcatca
    11161 aacagtcaca tagtcgattg cttctgcttg cggaatcgtt tcatctacaa agtagacttt
    11221 ataccatgtc aagaaactga agattgtcca gatttttctt tgctcttctt tacgtccttc
    11281 gtgatggtca tctagcaact tcatgatttt ttcttgatca aaaaattctt ttgcaaaatc
    11341 tgcttcgaat aatgctttga cttgttcgta gccgtgttct tctttcaacc aagctttgat
    11401 tggcacaggg aatcctaatt tcacacgatt cgaccattct tccggaagat gtttgttcgc
    11461 agcttgtctg aaaatatctt ttgtgttgtt ttgattcaat aaaaacttac ttggtatttg
    11521 ttgagccaat ttcatgactt cgatatccaa taaaggtact cggacttcca aagaacttgc
    11581 catagaaagt ttatctgcct ttaacaagat atcttttggc atccattgat gaagatcgac
    11641 atattgcatc ttattgactt catctttgat tccttctgtt ttctcgtaat gaacagtcat
    11701 gatttcatcc acagatggtg ctgtttgata ttttggtgtt aacacttctg atgcttctga
    11761 ttcttcgaac actcgagcat gaccgataaa atattctttt gcaggagcaa gtgattcata
    11821 taaatggatc cgtccatgga agtttttcat ttttccgatt ttacgtccta aattatagcg
    11881 tgttcctttt ggtaattttt tcaatccttg agcaatcaca cggatgaatt ttgaattcgt
    11941 atggaatcca tacgtttggt atcccgcaaa cagttcatcc gccccttctc cagattggac
    12001 tacccgcaca ctttgtgaag caagatttgc taaaaagtac aaaggtacgc aagaaggatt
    12061 tgaatcaggt tcatctaagt gatactggat caaagggaat gctttgaacg acatgtctcc
    12121 gtcgatcact gctgctgtat tatctaagtc caatagttcg gttagttttc tggcttcaat
    12181 cgcttcatta taggttttat catcaaaccc gattgagaaa gaatgatctg gtttcaatac
    12241 agaggttaca taactggaat caaccccgct ggataagaaa gaaccaactt ctacatcact
    12301 gaccgtatgt gcttcaatag aagcttgaac ggtttcgtcg attttttcga tccattcttg
    12361 tcgggaatgc gtttcttttg tttcaaaatc tgcatcccaa tattgctgaa tgtctatttt
    12421 tccatcttta tacgtataat aatggccttc aggcaaacga tagacatctt tgaagaatgt
    12481 ttcgccgttt aatggagaat attggaaagt catataaggt ttcaacgctt ctttgttcaa
    12541 ttctttgtta aaatcaggat gaggcagaaa acttttgatt tctgaaccaa acataaatgt
    12601 tccgttcatt tctgcataat aatatggttt gatcccaaag tggtctcttg caccgaataa
    12661 ctcattcttt tcattatccc aaatagcaaa agcaaacatt ccgcggatct tttgcaataa
    12721 ttctgttccc cattcttcgt aaccatgcaa aaggacttct gtatctgcat gtgtttgaaa
    12781 aacgtggcca gctgcaatca attcttctct caaaggctgg taattataga tttcgccatt
    12841 aaaaataatg atctttgtcc gatcttcatt gtaaataggc tgagtaccgc cctctaagtc
    12901 aataatactt aatcttctga atcctaaagc aacatgttta tcgatatatt ctcctgaact
    12961 attaggtcca cggtgaacga ttcgatccat cattgtatta ataattgttt ttttgttgtc
    13021 cttgtcgtta acaaaaccta caataccgca catttaactt tcacatccta atataaaatt
    13081 actgtttatt tcattcatat gcatcttatc atagaataaa tagcggacga atattttctc
    13141 tcttacaatt tcgtcacgtg aaaacaaaag atgaaagttt ctgtttttac agaaaataaa
    13201 gtcccaatat ctaatttacc atgttttctt ttttgaggct accttattct cttcgtgtta
    13261 aaaaaatatt agtggatgct atttccaaaa aaaccagcaa aaaaagacac gacctaaaga
    13321 tcgt
//
LOCUS       DACDRK010000070        13198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDRK010000070 DACDRK010000000
VERSION     DACDRK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.070"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            107..1248
                     /locus_tag="IWJ77_002155"
     CDS             join(107..335,335..1248)
                     /locus_tag="IWJ77_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9453739.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1424..3631
                     /locus_tag="IWJ77_002156"
                     /pseudo
     CDS             1424..3631
                     /locus_tag="IWJ77_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
     gene            3711..4106
                     /locus_tag="IWJ77_002157"
     CDS             3711..4106
                     /locus_tag="IWJ77_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453740.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4431..5048
                     /locus_tag="IWJ77_002158"
     CDS             4431..5048
                     /locus_tag="IWJ77_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9453741.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5089..5388
                     /locus_tag="IWJ77_002159"
     CDS             5089..5388
                     /locus_tag="IWJ77_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453742.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5422..5589
                     /locus_tag="IWJ77_002160"
     CDS             5422..5589
                     /locus_tag="IWJ77_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9453743.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5634..6239)
                     /locus_tag="IWJ77_002161"
     CDS             complement(5634..6239)
                     /locus_tag="IWJ77_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9453744.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            6299..6757
                     /locus_tag="IWJ77_002162"
                     /pseudo
     CDS             6299..6757
                     /locus_tag="IWJ77_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6922..7149
                     /locus_tag="IWJ77_002163"
                     /pseudo
     CDS             6922..7149
                     /locus_tag="IWJ77_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7275..8246
                     /locus_tag="IWJ77_002164"
     CDS             7275..8246
                     /locus_tag="IWJ77_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9453745.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            8386..9699
                     /locus_tag="IWJ77_002165"
     CDS             8386..9699
                     /locus_tag="IWJ77_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9453746.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            9742..10608
                     /locus_tag="IWJ77_002166"
     CDS             9742..10608
                     /locus_tag="IWJ77_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9453747.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            10605..11453
                     /locus_tag="IWJ77_002167"
     CDS             10605..11453
                     /locus_tag="IWJ77_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9453748.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            11467..12939
                     /locus_tag="IWJ77_002168"
     CDS             11467..12939
                     /locus_tag="IWJ77_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9453749.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            12956..>13198
                     /locus_tag="IWJ77_002169"
     CDS             12956..>13198
                     /locus_tag="IWJ77_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9453750.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         4305 a         2157 c         2696 g         4040 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccattacta atctagtagc
     1441 taattttgga cctaccactg aaaaaatgta cgataatcag gttactccta aatggggcaa
     1501 aaatctacct aaaaatattg tatttcttat tcaaggtgcc ttttggtgta aggggattaa
     1561 tccaggtggc ttcgatgggg tatatactcc atatttagat actgctgtaa aagagttgca
     1621 gactgatgca gggtttaatg gaagcgctgt aactggagtt ttggatgcga aatgggccaa
     1681 agcgttattt gatatgagtg cttttgttct tgttcccggc ggagatagta aaattagaaa
     1741 aatgcagcaa tggttaaatg taaattattt agaatacacg ggaataatgc cttgtgatgg
     1801 tatttatcaa agaaattcaa atcaagcatt aatttttgca ttacaagctg aactaggata
     1861 ttctcccagt gaagcaacag ggtcttatgg taatgggaca actagtaaaa cttatcctgt
     1921 gtctgaaggt aactcgggaa actatgtaag aataattcaa tatggattat atgttaatgg
     1981 ttattttgaa gacggtacat tcgatggtat tttcactaaa tatatgggtt tagaagtact
     2041 ggcattccga aaatttatgg tattgcctga atatactgaa atagcagatg ttgttgtaat
     2101 aaaaggacta ttgtctagcg ctggagatat tagaagatct gcagatggcg cagatacttc
     2161 tacgcaatta actagatctc aaatccaaac attggttgat aatgatataa aaatagttgg
     2221 tagatatctt actggtactg ttggaattgg aaaagatgaa cgaaataagt atctaactac
     2281 agaggaattg aataatattt tttctaaaaa tttatctgtg tttcctatct atcaagatgg
     2341 aggtgcagca ttagcttatt ttacttatga tcgtggctta tcagatggta agaaggctat
     2401 agatgcagca aaaaatctaa atataccatt aacaacagtt atttattttg ctgttgatct
     2461 agacatgtta ggggaagaaa ttcttgcata tgctggagaa tattttaagg gggtatctgc
     2521 tgtaatgtca tatagcgggt atcaaacggg tgtttacgga acaagaaatg tttgttctca
     2581 aataatcaat aacgggtatg cttcgtttgc atttgtttca gatatgtcga ctggttatag
     2641 tgggaattta ggttttccta tgcctagaca atggtctttt gatcaatttg ttgaatttac
     2701 cataggaagt ggcaacggag ctgttggtat cgatttaatc gctacatcgg gtagagatag
     2761 cggatttaat gagttatcaa atgatactaa taatgataat tacattgcca aatacaatca
     2821 gaaggttata aatcagatgg tacgtgcgta tcaatatcta tcacaagctg gtcttgataa
     2881 tccttggaac cctcatttga cattttatag atatgtaaat tattctggtt tgtcatggga
     2941 catcatttct tctcctgtaa cagagcatga tagaaaaatt tatgatgaat ataggaataa
     3001 attaactaat agcgagggcc tgtataatta ttttatagat cctaatactg gttcaataat
     3061 aggtttgcct cacttaattg tgacgttaca atcacagatg tttctagccg acacaatcaa
     3121 tgatagtgtt tctgactttg caggttggtt aggagattta atgacttgct ggggtgaagt
     3181 taagaaatta ggaatacaaa tggagcaagg tgtatttgca ttagtaggta cggctgcagg
     3241 tggaacattt agtctagaag atttatacca agatgttgac gcggtaaatg tttatacctt
     3301 agcaaaaaga aattctgatt caacagcttt atcggcttta aaaacgtact attgtgataa
     3361 gggtgctatt gatagattta atcaatttgt taagaacaga tttggttctt cagataaaat
     3421 cgcttcagta acaaatagtt tattagaaaa tgacgaaaca gttgatatct taggtgtagg
     3481 gataatttca tttttcattg ataaaatagt taataattca cttgggacag attttactta
     3541 tcttgatttt attggagatg aggggaaagt tgcaaatgga ttttctgaaa aaattattca
     3601 atttcaagaa aatagaagta aacttaatta agaatcgttt actatttaaa cagtgtcctt
     3661 ttccttttta aatatatacg ataaaataag tatatgaatt gtaaaaggac atgatgatta
     3721 tggaagaaat agaatggaat aatctgactc aaaataataa aaataattct ggatgtggaa
     3781 ttatctttat gtttgttata gttttgggaa tagtagtact tttaataact gtcaagatag
     3841 tagattactt tgcgaatact cctgaagaac aaatacagag aaaacttcct gagtttgaaa
     3901 gagtaccacc ttatgatcca agagataatt catttcaagt aaaaaaatta aaagagtctc
     3961 caaatgaaat gtggtttgaa gttacagttg aatcaagcga cttcaaagaa aaaaagaaag
     4021 aatacaatta tattgtaaag gcaaaagtat caaaaagatt tatttttaac cctaaattag
     4081 aaatatatag tgttgtaata gaataatagt gttaaaaaaa ttgtaaaagt taaagaagtc
     4141 aaattctgat gtgaaaaata aaatatacca tggaagacat aaaagaaaaa ctggataact
     4201 agtttattta atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat
     4261 ataatcatgc gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga
     4321 ttttctttgt ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt
     4381 aatctgattg ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat
     4441 ttttttatat ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga
     4501 aggaattagg aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta
     4561 aacgccctga atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac
     4621 aatcattaga tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa
     4681 ctaaggaaaa gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt
     4741 acaagcaact agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct
     4801 atatggccga agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg
     4861 cgaaacaaaa gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg
     4921 cgatctattc tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg
     4981 gcatgaaacg aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact
     5041 tttcttgata gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg
     5101 aatcaaaggg tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt
     5161 ttggttcact ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg
     5221 gtttgtggtc agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa
     5281 accgcgcggg acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt
     5341 tatgcgttaa ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa
     5401 tttacaagga ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata
     5461 actagtttta atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa
     5521 agtgtttatc aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat
     5581 ggaacataga aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa
     5641 tcattcgctt tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc
     5701 tctgcaactg gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt
     5761 tgcggatctg ttgagttaat agaatactcg attggtcgtc ccttatatcg gccggctttt
     5821 tgagccaata atactccttg ccgctgacgt tcacgaattt tctcacgctc gttttgtgca
     5881 atataactta gcagtgataa aaacatatcg aacatagcgg tatccaggag ttcatttcca
     5941 gtattaaaat ctaaaaactg tgcatctaat atttttaacg ctactttttt ctgtttcata
     6001 aaagcaactg tattttttat atcttcataa tctcttccta aacgatctag tgatgtaacg
     6061 atgacgcaat caccttcacg gatataattt agtaattttt gaaactcagg acgctcagta
     6121 tctttaccac tggctttttc ttggaataat ttatcaatgt taagtccttc catcattgcc
     6181 aattgtcgat ccaaattttg atcttgtgaa gatactcgaa tatagccaat ttttcccata
     6241 aaaaactcct ttttaaaagt gatataattt acatgtaaaa tttacttgta gttttactgc
     6301 tatcatagca tttttaaaaa taaattttaa gtctacttat aatttacttt ggaggttttg
     6361 aatgaatgaa attaagcgca ataaaaattc ttttggtgtt tatttaggag agcctagctc
     6421 ggaacagtta aataaaggtg aagctcgtca tgctctatgt cgaaatatat tttatggtag
     6481 aaaaggcaaa ctttatcaaa cttattacga cggtatggag gaacagctca acgcattagg
     6541 attagtaact aatgctatca tatattggaa ttcactatat ttagaaaaag tctttgaaca
     6601 aattaaaacg gaagggttcg actgttccga agaagtgtta cgcaagcaat ttccattaat
     6661 tagtgatcac attaattttg ttggcaaata tagtttccgc tatgaaaaag aattatcgaa
     6721 tggaatacta cgtccgttaa atatagagaa ttattgagaa tgttcgtctt ttggttccta
     6781 ccgtgcattt tcgcacggat gttccagata ctatttttga atgtataaaa ttgaaaacac
     6841 aagaatacta ataaatcaac gattcttgtg tttttaattt ttcactaccg tgcattttcg
     6901 tttctatgtt ccatgtaccc caatcttgaa aaattaatta aagacttaga taaagccatt
     6961 gacgacttag tcgtggtgct accggagtat caatgtttaa ccagtatccc aggtattggt
     7021 cctgtttagg ctgctggaat actcgctgaa attggtccaa ttgaacgttt ttctgatgaa
     7081 ataaaattga caaagtatgc cggcctatat tggcgacaga gtcaatccgg taaatctgaa
     7141 tacgaaaatt tttttacgaa aagcttcacg tctaatatct attttaaaat aaaaatattc
     7201 ctgctaattt ggaatcgttt ccttcttttg gtataataat acctgaaatt cttttgagag
     7261 gagttgttcg tgaagtgaaa actactatga aggatgttgc tgcgttagca ggagttggtg
     7321 tgggtacggt atctagagta ataaatggag taaaggtgaa agaggttacc aaagaaaaag
     7381 taatggctgc aattagtgag cttaattatg agcctgatga atatgccaga ggtatgaaaa
     7441 cgaatagtag caatacaata gctcttattt tacctacgat ttggcatccc ttcttttccg
     7501 agtttgccta ctatgtagag gaagctctca gccaaaaaaa ttataagctt tttttgtgta
     7561 atgcgaacgg cagtgcggaa aaagaaaaag aatatatcca aatggtcaag cagagtaaag
     7621 tagacggaat tataggtatt acctattcag acattgataa atatgtatca tcaaatttac
     7681 catttgtcag tatagaccgc catttttcgg aacaggtttc ttatgtaacg gcagataatt
     7741 atttaggtgg gcaaattgcc gctcaagaac tacttactcg taactgtaaa aacttgtgct
     7801 atgttggggg tgtttcacct taccctaacg aaacaaacaa tcgtaaagct ggtttttaca
     7861 atatttgtga ggaaaataaa atagctgtga aggtattgga tatgcctgaa ccaattagca
     7921 atttagagga acaattgctc gctttttttt cggataatcc tgaaattgat agcattttta
     7981 ccatcaatga tttcatggct ttggatgtga taaaagccca aaaaaaacta gggaaggaag
     8041 cacctaagga ttttcaaatt attggttttg atggagtcaa gctatcgttg caaaaagact
     8101 atttactatc aacgattgta cagccgatag aggaaatggc caaggtttca gtagaaacac
     8161 tcatggcgat gattttaaat gaagaaccgc ctaagcgtgt ggtagtaccg gttttctttc
     8221 acgctggaga gacgactaaa gagtaaaaaa ccggtttata acgatttttt tgagataggt
     8281 tgacagaaag tgatttcaat gatatcttaa aaccaattaa ttggaaacgt taccaattaa
     8341 ttgaaaaata aaacggaaac gtttcaaaaa ataggaggaa catcgatgaa aaagagaatg
     8401 ttagcaattg cagcaatttt agcattaaca gctggaggac ttgcaggatg tggcagtaag
     8461 gagaaaagtt caggtagcag tgatccagga gaaattaaga tttgggttca gttttctgat
     8521 gaaacgcctg aaggaaaagc ttgggcagag attgtgaaca attttaatga tgaatacgag
     8581 ggtgaatata aagctgttac tgagtatatt cctcgtagcg gaagcggcgg tggatatgag
     8641 gataaagtaa atgctgcaat tacaaccaat acccttcctg atgttattac gttagatgga
     8701 ccgaatactg cagcttatgc tcattcagga gtcattgctc cattagatga ttatttgaaa
     8761 gatgcggaca tggatgatgt attagacagc attattcagc aaggaaccta cgatggcaaa
     8821 atgtatgcgt ttggtttctc agagtccagt gtgggtgtct attacaataa gtcgctgttc
     8881 aaagatgctg gaattacaga agatcaattg ccgacgttag aagatccttg gacttggacg
     8941 gaatttcaag caatttgcca aaagctgact gataaataca aaacgcctgc aattgatatg
     9001 caaatggcgt cgggagacga aatgctgacc tacgcatata caccatttat atggtcgaat
     9061 ggcggcgatg tcgttaatgc tgatggaacc aaagccgaag actattttaa cggtaaaaaa
     9121 tccgttgaag cgttggaatt tattcaagaa ttggttgaaa aaggttacac cacaatcagt
     9181 cctattgaaa aaggatttga aactggcaaa tatgccatga tgctgagcgg ttcttggaca
     9241 atggcagatt tagaggaaaa ctatcaagag attgattatg gtattttgcc ttaccccgtt
     9301 tcagatgcaa ctggggagtt agtttcacca accggaagct ggcagttagc aatgactgag
     9361 aaaacagaga aaaaggatgc ttctgcggcc tttattaagt ttgcaaccag tacagaatct
     9421 agtcgtatta tgagtttagg gaacagtgtt ctacctatca gaaattcaac agttgattta
     9481 attaaagatg aagtgtcgga aggaatgcag gtattaatta agcaaaacca actttctgga
     9541 catgcccgcc cagtggttgt agcttatccg caagtatcta gagcattcca acaagcagtt
     9601 caagatatta gctacttcga ggaaaaccca gatgtacaaa aagtagcaga tagtcgcgct
     9661 gctgaaatgc aacaggcaat tgatacttca ctaaaataga agtttctaaa gggggccaat
     9721 caagtgttaa aaaaaccgac aatgaaagac aatttgatag gttacggctt tctagcgcca
     9781 gctttgctgc tgctgacgat ttttctgatc atacccgtaa tcatggtaat ttattatgcc
     9841 tttactgatt attatttgtt aacgccagat gctagacaat ttatcggctt agaaaatttt
     9901 aagcgactgt tcgctgatcc aatttttgta aaaagtattt ggaacacggc taaatttgtt
     9961 gtttggatta ttccggtcca aataggggca gctttaggaa tggcacttat tatcaacaaa
    10021 aatcgtaaag gaaatatctt ttttaaagta gccttttttg cgccagtcgt gatgtcctta
    10081 gtcgttattt cgattttatg gttgtacttg ttaaatccta atgaaggtct ggttaatacg
    10141 ctatttgcaa agattggaat tgcttcgcag ccttttttga ctagtcccaa acaagcaatg
    10201 tatacgcttg ttttcgtatc ggcatggcaa ggtgcaggat atcagatgct gctgttttta
    10261 gggggaatgc aaaatattcc gcaagacgtt tatgaggcag ccgaaattga tggtttttct
    10321 aagtttcagc aatttcgcta tatcaccatg ccattattaa agcccacggc gatttttatt
    10381 ttacttacca cattgatttc ggcctttaaa ctaatcattc agccaatggt aatgactcaa
    10441 ggtggaccaa tgaattctac tatgacaatg gtttattaca tttaccaaac tggatttaca
    10501 gaccgtatgg taggctactc cagctccatt gcgttaatct ttaccactat gattggttta
    10561 attacaattg ctcaaagaaa attggtaaag gaggatgatc cagcatgacc aagaaatttg
    10621 gaccgctaat gatcgtagaa tattgtttgc tgttcctttt agcggcatta ttcattttcc
    10681 cgatgctgtg gatggtagtt tcctctatga agcctgaagc agatgtctat caaaatttag
    10741 cttccttcaa agctttttta cctagtttga atccagctaa ttggtttaaa acttatcaag
    10801 aggttatttc ccggttcagt gtgggaactt atttattaaa cagtattttt tatggtttaa
    10861 cctttgcctg tgggtcaatc attgtaaact ctttggcggg ttttgccttt gcgaaggtta
    10921 atttttcagg caagaaaatt atctttggag ctttgttagc tctgctgatt gtcccgatgg
    10981 agacggtttt aattccgcaa tttaccatcg ttaattcact aggcttagtg aatactcgct
    11041 tggcagtgat tttaccagcg atggccagcg cgtttaatat ctatctgttt agaaatttct
    11101 ttatcgcgat tccagaagaa atcattgaat cagcgaaatt agatggtgca aatattgtta
    11161 aaattttctt tcacatcatg ctgccaatgt ctaaaccagc catagccaca gtaggtgtct
    11221 tatcctttat ctccagctgg aatgattata tttggccgtt aatggtctta actgacagca
    11281 gcaaattttc aatgcaagtg gcaattacta cgatcaatac tacacagcca gtttatatca
    11341 atcaagtgat ggctgtttta accattgcaa cgattccact gattattgtt tatatcgttg
    11401 cacaaaaata tattctgcag ggacttggcg gttctggaac gggtataaaa taggagtgaa
    11461 tagtgaatgt tgcaaaaact tttaaaggcc aatgagttta ttgagaaaaa tgctcaaaca
    11521 gtcaatcaag aataccggaa taaatttcat ttgatggctc cgattggctg gatgaatgat
    11581 ccaaatggtt ttgtttatta tcaagaggag tatcacttgt tttatcaata ttatccttac
    11641 gacagcatct ggggaccaat gcattggggc catgctaaaa gcaaggatct aattcactgg
    11701 gagcatttgc cagtagcact ggcaccaggt gaaagctatg acaaggaagg ctgtttttcc
    11761 ggcagtgcca tagaaaagga tggcaagctg tatttgattt atacaggaca caatgttgtg
    11821 gaagggcaag ttcgacaagt tcagtgtttg gcagtttcag aagatggcat tcactttgaa
    11881 aaatatgccg gaaatccaat catcgcagaa gatcacttag cttctgttgc aacgaccgaa
    11941 gatttccgcg atcccaaagt gtttcagcga aaagaagact attttactgt agtcgctact
    12001 aaaactgcag atgctcgtgg acaaattgtg atgttccatt ccaaagattt actaacttgg
    12061 gagttttatt ccgtattgct ggagggaact ccagaacaag gcattgtatg ggaatgccca
    12121 gacttgtttg aactagatgg caaagaagtc ttgattcttt ctcctattca aatgactcca
    12181 caaggcaatg cttaccaaaa tacgaactca actgttgctt tcatcggtac tgtcgattgg
    12241 caaacaggca aatttctagt agagaatttt catgaaatcg atggcggttt agatttttat
    12301 gcaccccaga cctgtgaaaa cgaagcaggc caaagaataa tgattgcttg gatgcaaatg
    12361 tggcagcgta aaatacctac gaatgatctg ggccatggtt ggtcaggttc catgaccttg
    12421 cctagagagc tgagggtggc tgataatcaa ttgttgcaac ggccagtttc atcaatttat
    12481 caagtaattg atccgcagga aaggattgaa tgggcgaaga ctgaagagaa tcgcttctta
    12541 ctgaaggact tagtatatga ccagcactat ttacgcttgg aaatcaattt aaaacaggca
    12601 acgaaactag aacttcacta tgcgaaggga cccgaccata cattactgct taactatgat
    12661 gttgaacggg agtgctttac tgcttccagg gaacacatgc cgtatgattt atcaggagct
    12721 gaaaaggaag tgttgaaaaa gcgcagtgtg acgattcctt tggaaaataa ttgtctaacc
    12781 atcgaaattt tccgtgatac ttcagctatt gagctttttc taaatgatcg tctgactatg
    12841 agtacaactt tttatgagac agaaaaagga caggattttc ttttgctggc agacagtgaa
    12901 gtaacctttt cattggaaag cggcttaatt aatctataga agggagagct cagtgatgat
    12961 ttatggttca atcgaagcag gcggcacaaa atttgtgtgt gccattagcg atgaagaaat
    13021 gacgatcaag gaaagagtga gctttccgac aacgacacct gaggaaacaa tacctttagt
    13081 cattgacttc ttcaaacaat accaagcaga tttagcaggg attgggattg gctcatttgg
    13141 gcctatcgat atccatcgcg attccgccac ttatgggtac attacctcga ccccgaaa
//
LOCUS       DACDRK010000071        13147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDRK010000071 DACDRK010000000
VERSION     DACDRK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.071"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IWJ77_002170"
     tRNA            complement(77..149)
                     /locus_tag="IWJ77_002170"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IWJ77_002171"
     CDS             530..1099
                     /locus_tag="IWJ77_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9453751.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IWJ77_002172"
     CDS             1086..1946
                     /locus_tag="IWJ77_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9453752.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IWJ77_002173"
     CDS             2136..2840
                     /locus_tag="IWJ77_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9453753.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IWJ77_002174"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IWJ77_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9453754.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IWJ77_002175"
     CDS             4677..5993
                     /locus_tag="IWJ77_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453755.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IWJ77_002176"
     CDS             5994..6863
                     /locus_tag="IWJ77_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453756.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IWJ77_002177"
     CDS             6918..7727
                     /locus_tag="IWJ77_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9453757.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IWJ77_002178"
     CDS             complement(7830..8459)
                     /locus_tag="IWJ77_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP9453758.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IWJ77_002179"
     CDS             8629..9921
                     /locus_tag="IWJ77_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9453759.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IWJ77_002180"
     CDS             complement(9997..10659)
                     /locus_tag="IWJ77_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9453760.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IWJ77_002181"
     CDS             10884..11168
                     /locus_tag="IWJ77_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9453761.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IWJ77_002182"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IWJ77_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9453762.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattca
//
LOCUS       DACDRK010000072        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDRK010000072 DACDRK010000000
VERSION     DACDRK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.072"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWJ77_002183"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWJ77_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9453763.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWJ77_002184"
     CDS             complement(322..891)
                     /locus_tag="IWJ77_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9453764.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWJ77_002185"
     CDS             1077..1979
                     /locus_tag="IWJ77_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9453765.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWJ77_002186"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWJ77_002186"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9453766.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWJ77_002187"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWJ77_002187"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9453767.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWJ77_002188"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWJ77_002188"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9453768.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWJ77_002189"
     CDS             8547..8732
                     /locus_tag="IWJ77_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9453769.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWJ77_002190"
     CDS             complement(8794..9990)
                     /locus_tag="IWJ77_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9453770.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWJ77_002191"
     CDS             complement(10160..11596)
                     /locus_tag="IWJ77_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9453771.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWJ77_002192"
     CDS             11889..12698
                     /locus_tag="IWJ77_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9453772.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDRK010000073        12695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDRK010000073 DACDRK010000000
VERSION     DACDRK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.073"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(206..568)
                     /locus_tag="IWJ77_002193"
     CDS             complement(206..568)
                     /locus_tag="IWJ77_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453773.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(642..1022)
                     /locus_tag="IWJ77_002194"
     CDS             complement(642..1022)
                     /locus_tag="IWJ77_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9453774.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1104..2279
                     /locus_tag="IWJ77_002195"
     CDS             1104..2279
                     /locus_tag="IWJ77_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9453775.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2356..2940)
                     /locus_tag="IWJ77_002196"
     CDS             complement(2356..2940)
                     /locus_tag="IWJ77_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9453776.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3141..4139
                     /locus_tag="IWJ77_002197"
     CDS             3141..4139
                     /locus_tag="IWJ77_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9453777.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4251..4760
                     /locus_tag="IWJ77_002198"
     CDS             4251..4760
                     /locus_tag="IWJ77_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9453778.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4790..5449)
                     /locus_tag="IWJ77_002199"
     CDS             complement(4790..5449)
                     /locus_tag="IWJ77_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9453779.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5451..6215)
                     /locus_tag="IWJ77_002200"
     CDS             complement(5451..6215)
                     /locus_tag="IWJ77_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9453780.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6223..6900)
                     /locus_tag="IWJ77_002201"
     CDS             complement(6223..6900)
                     /locus_tag="IWJ77_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9453781.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6937..8322)
                     /locus_tag="IWJ77_002202"
     CDS             complement(6937..8322)
                     /locus_tag="IWJ77_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9453782.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8532..8616)
                     /gene="ffs"
                     /locus_tag="IWJ77_002203"
     ncRNA           complement(8532..8616)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWJ77_002203"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8639..9154)
                     /gene="tadA"
                     /locus_tag="IWJ77_002204"
     CDS             complement(8639..9154)
                     /gene="tadA"
                     /locus_tag="IWJ77_002204"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9453783.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9261..10118
                     /locus_tag="IWJ77_002205"
     CDS             9261..10118
                     /locus_tag="IWJ77_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9453784.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10199..10534)
                     /locus_tag="IWJ77_002206"
     CDS             complement(10199..10534)
                     /locus_tag="IWJ77_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453785.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10601..11971)
                     /locus_tag="IWJ77_002207"
     CDS             complement(10601..11971)
                     /locus_tag="IWJ77_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9453786.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12049..12585)
                     /locus_tag="IWJ77_002208"
     CDS             complement(12049..12585)
                     /locus_tag="IWJ77_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9453787.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3788 a         2574 c         2111 g         4222 t
ORIGIN      
        1 tcaactataa attaagcatt actttgtgaa atgatttttc gatcaattat tattattcgt
       61 tatacaaccg ttttcaacat attttgaaaa taaaatgcac taaaaaacta tctttagcag
      121 aacatttcat tgtatttttt ggaccaaaca ataaaaaaag aagtttgaaa aaaattcaaa
      181 cttctttgtt cgcttaatct gtattttaga agaaaggcac tccctctact tcgtaagttc
      241 caagaacaga gcgcatttga cctggttctt cccaaatata tccttcgcct aattcttctc
      301 tttgattctt ttgttcagtt tcaaagacaa acaacggaac attttcctct ttaatatgtg
      361 tatttgtcaa ttccatcaaa ctaatcttgc taacatcgat atgaactatt tcttttaaga
      421 aattaagtat attcgctaaa ccagtctttc cttcagaaag ttccgctaac gcaaattcca
      481 acttgtcatc aattgaatgg accaaaaatt ttttagtacc atcctccaaa tgcaacataa
      541 ttgtaccagc aactcttttg cttgccaaaa tttccacctc gatcaacttg ttctatggct
      601 tacataaaaa ttctagcaca ttttcagaaa aaatacactt tttatttctt attttctttc
      661 cgttctagtt cagataatgc ttcttctatc caaataggta actgctccgc aatagtggta
      721 aatccgatct tacgattttt attcgtgaaa tacctttttc tgcgatatgg aacagctgga
      781 tttgtttttt ctaacgttct taaagataaa ctgatttttt tggaatattc atcaatgtcg
      841 atgatctgaa ctgttactgg ctgtccgact tttaacaatg aatggatatt tttcgtataa
      901 ccggattgta cttctgaaac atgaataagt ccttgcgtct cgttatcaag agaaacgaat
      961 gcgccataag gctgtatccc tgtcactttt ccttcaatta tttgtccaat tttataagtc
     1021 atctacttcc ctctcttcgc atttatcatt atactgtaaa tagctcaaaa aaagaagcga
     1081 cgaaaatgac tggaggtctt aagatgatcg aatttgataa acagatttca agaaaagata
     1141 cagaaagtgt aaaatgggac gcaattgctg agacatatca aatggatgac ttacttcctt
     1201 tgtgggtagc agatatggac ttcttgtctc ccggtggggt agctaaagcg tttagtgaat
     1261 acatcaaaca tggaatcttt ggttatgcca ctctgtctga taaactatat caggctatca
     1321 ttcattggga acgccaacac catgctgtgg agttgacgaa agaaaatatc gtttttacaa
     1381 gtggtgtgtt aaccagttta gctgcagcag tacaaacttt tacaaaacca ggcgattcta
     1441 ttttgattca tgacccagtt tatcctccat tttcctctat tatagaaaca aatcaacgtc
     1501 acatcgttcg aagctctttg ctagaaaaaa ataatcattt catcatggac ttgtctgaca
     1561 tggagaaaaa aatcgaagaa aacaatgtaa aagcaatgat tttatgcaat cctcacaatc
     1621 ctggagggcg tgtttggagc aaagaagaat tgaaacagtt aagcgaattg tgtttaaaac
     1681 atcaagtgat tctgtttagt gacgagatcc atcaagattt aactttattc gatcatacat
     1741 ttacttccat gctaacgatt gatcctaatt tagatgatct actagttgct tttacttccg
     1801 caaccaagac tttcaatcta gctgcaatca aaaattcaat ggtatttatc aaaaatccag
     1861 agatgaaagc tgattttgaa aaacatttag tgatgaacca acagcacgaa atcaacactt
     1921 ttggactgat tggaacccaa gcggcttatg agacgggtgg agaatggtta agccaactga
     1981 ttccttatct agaaaaaaat gttcaaacag ttaatcagtt ttttgaacag tatcttccga
     2041 aagtgagaat aatgaaccct gaaggaactt acttaatgtg gttagatttt accgcctata
     2101 tttcagacga ccaagaattg gagaacactc ttgttcggaa aggaaaagtc gtattaaatc
     2161 ctggcatcac gtttggtcct tccggtcatg ggcatatgcg cttgaatatt gcttgtccag
     2221 aagaaacttt actggaagga cttaatcgaa tcaagtatgc tttagagaca atcgattaaa
     2281 tcatttattc aaaataaaag gacgagacct tactaggttt cgtccttttt cataattact
     2341 ccgtaccata ccaaactatt ctgaaatttc aattgtttca atcactacat catgtacagg
     2401 tcggtcttgt ggaccacgtt gtacatttgc gatttcatct actgtgtcca tcccttctag
     2461 tacatgtcca aagactgtat gtcggaaatc tagccaagga gttccgccac ctttatacgc
     2521 ttcaatgatt tctgatggga atccagcacc ttctagttgt ccaagcatat tagctggcac
     2581 atttgtgtta tttacaataa agaattggct tccgtttgtg tttggacctg aatttgccat
     2641 agataaagct ccgcgaaggt tgaacagttc acgactgaat tcatcttcaa aactttcgcc
     2701 ataaatactt tcgccaccca ttcctgttcc agttggatcg ccgccttgga tcatgaaatc
     2761 aggaataacg cggtgaaaaa tcacgccatc ataataacct tttttggaaa gttcaatgaa
     2821 attttgaacc gtcttcggtg ctagttcagg gaataattga acagtaatgt caccacggtt
     2881 tgttttgata acagcttttg gtcctttttc actttttaaa tctagttgtg ggaatgccat
     2941 attgtttcct cctaatattt gcatacttca taataataat aacataatac ctaacctctc
     3001 gtaaaaatac tagatttgtt ttcttttatt tttcatcact agatacattc tataacttac
     3061 cgtttcgatt tcgaattcaa acatttttga gaagcattct caattaaatt ctgctatagt
     3121 gtgaaaaggg aggaattttt atggaaatct atgacattac aatcatcggt gcaggtcctg
     3181 tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc taaaacaaaa atcattgaca
     3241 gtctacctca acttggtggt caattagcaa ctttgtatcc cgaaaaatat atttacgata
     3301 ttcctgggta tccagcaata aaagcaagtg aattaatcga tcaattagaa aaacaattaa
     3361 ctacttttaa ccatacattc catttaaaag aagaagttct ctcgcttact cgtgaagacg
     3421 aagttatcga gattactaca aataaaggca tccattattc caaagcagtc attttagctt
     3481 taggaaatgg ttcattccag ccaagaaaat tgaatttaga taatgctgaa tcatttgaaa
     3541 accacggatt ggattatttc gtgaatgatt tgatgaagta tgctggaaaa aaagtcgcta
     3601 ttgctggagg tggtgattct gctatcgatt ggacgttgat gttagagcct attgcaagcg
     3661 aagtttactt gattcatcgt cgcccggaat ttcgtgcgca cgaacatagt gtatcacgct
     3721 tgaaatcttc atctgtcaac ttgcttactc cctatctcat cgatggatta tcaggcaata
     3781 atggggaact gacagatatc cggttaaaaa aagtgaaaag tgacgagacg attgatttga
     3841 tgattgattc tctgatcgtc aattatggat tctcttctaa tttagaacat ctttcttctt
     3901 ggggacttga tagtaccaga aatgccatta ctgtaaaatc agatatgtct acttctattc
     3961 caggcgttta tgcagctggt gacatctgta cctatgaagg aaaagtgaaa ctgattgcta
     4021 ctgggctcgg tgaagcacct actgcagtga acaacgcttt acactatatt aatccaaagg
     4081 aacgaactca gcctggtcac agcacaagct tatacgataa aaatcttcgt cctagttgat
     4141 tttttatatc tctttgcatt tcaagttaaa cccgaaagca gtttcttgca tttttttcat
     4201 gttccgttaa catgaaatag agaaagaaaa aataaagggg gagcaataat atggtcgtca
     4261 aaactgagac tttagaagaa aaggcaagag aactattgaa ggaacgtggc gtaactgtag
     4321 aagatatcgc acaacttgtc atgtttttac aaaaagacta tatttctgat ttgactttgg
     4381 cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg tgaagtacat aacgccatta
     4441 tcactggaat ccaattagat atattagcag aaaagaaaaa attgatgagt ccgttgcaac
     4501 atattatcga agatgacgaa ggcctttacg gtatcgatga gatcctagcc ttgtcaatcg
     4561 tgaatgttta cggtacgatc ggctttacga attatggtta tatcgacaag gtgaaacctg
     4621 gtattttaaa agaactaaac agccacgatg gcgttcatgt ccataccttc ttagatgata
     4681 tcgtcggagc gatcgccgct gctgctgcca gtcgattagc acacaaagaa ccagaaaaaa
     4741 gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa aacgtctatt tagacgagtt
     4801 ctttcttttt tgtttgtttc aagctatttt ttcctaagaa aagaaaaaca gcaaaaaata
     4861 attctaataa tacaaaaaat aagataaagc tatgcatgaa aaattcttct ggaagtacat
     4921 tgatgattgg atcagtaacg ggatcgaata accagtcatc gttgttgaaa aacgtttcat
     4981 gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa aaaaccgaaa acaactggca
     5041 gtaacatgcc gatttgaaac ggacgaatca accgccaaag ccgatcattc ttttgcaaat
     5101 atttgataaa caaaatactc gggatcaaag tgatgaaaaa cacagcataa tccactaaaa
     5161 acaaaatttt cacttcataa aagtgatggg ctccgctagc agaaactggg aaatctggca
     5221 agctgaggat ggttttgaag ggattattga gataattcat taggtgatca aaatttttca
     5281 gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg caaatgtccg atatcaaata
     5341 cgtatagtgg tcgaaagttg atcgtcacac caactgccag actgatcagt gtcagaaata
     5401 ggctgaacaa tccagcatat tccatccact gccatctcga atttttcata ttagaagctc
     5461 cattcatcca gtgattgttt cacataggta gctgggatag gcaacccagg tacatcttct
     5521 tcttttgtaa aacctgataa aacgagcaaa gtatctatat tattgcgtat cccagcttgg
     5581 atatctgttt catagttatc tccaaccatg attacttctt ctttttgcag ccctaatact
     5641 tcaacggctt tatccataat gatggcttct ggttttccga tgtagatcgg cggcgtttgg
     5701 gttgctgtct ctacaaaaga gatgactgac cctgctccag gaagaaggcc gcgttcagta
     5761 ggtatattct tatctgggtt cgttccaatg aaagtagcac ctttttgaat agcaagtgtt
     5821 gctttaacta ctttttcata ggtcagataa ttatctaagc ctaccactac atagtcagga
     5881 gcctcttctt cccatgtaaa tccggcagct aaaatcaagt caatcaatcc tgcttctccg
     5941 ataacataga cttttttccc ttttccatct gctttcataa aatcgattgt tgccaaagta
     6001 gctgtgtaga ctgtctctgg agctacatga atgtcaaact catctgctag acgattagct
     6061 actgtttctg gtgacttagt cgtgttattg gtaacaaaca agaaaggtaa ttttcttttt
     6121 tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt gtgtcccacg ataaatcgtt
     6181 ccgtctaaat caattaaata tcctttatag tccattcatc cactactctt ccctttttct
     6241 gatcgtaaat gctgtttttg ttttttctac ttgtttaggt tgttcttgtt tttttacttt
     6301 ttcagaggtt tttgctgact ctgtttttcg atttttaata accggttttt tgcgattaga
     6361 tgcgactggt ccttttttct catcgatata cgcacgatta tggttttttt tcttttttcg
     6421 gcgctgagtt gtcttttctt ttttaccgcc gattctctcg atgacaaaat acgcacaacc
     6481 aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg attcgctgat ctggatgcgc
     6541 ccgtcgatta tccgcttgga aaaatccttt taggcgtagt tgttcataac cccaatctcc
     6601 tacgatataa tcatatcggg ctagtacatc actgtaacgt tctccaagct tttctgcatt
     6661 aaatccttca cgataatcgg acacaagccg ataatgtctt tcacctatca aaaaattact
     6721 gtcatctaac agtgtcacta gctcgccttt gactttttct gctttaggct cctcaacgat
     6781 ttcttccaaa gcagctgtca gttcttcggt gactgttgtt tcctgttcag ctgatgattt
     6841 tttcttagca atttctgttt tatgctcaac gttctgctgt tcattttgtt ttgcagacat
     6901 gcgttcacca cctatctttt ttattatacc ttgtttttat ttgattgggt agtgagcttt
     6961 caagtaatct cctaccacac ttcggataaa ttcagggaac aaaaattcat tttttccagc
     7021 aatctcaatc gttggaaaaa atggaacaaa catcaaatga tctacggtag taaaagtata
     7081 atttttattc agatcgattg gcttgttctt ccaaaagact tgatggttaa tagaatcata
     7141 tgtgattcca ttatagacca tctgcccaaa aatcttaccg cgaaatccca ttcccttcat
     7201 aggatagtta cgtaaaaagt tacgattttt ttctatttct aaaactaagc ggatcagatc
     7261 atttccaccc aatgtgacat ttaacaagtg catcggatgc ggcaacgcag tgtgaagctg
     7321 atcctgattg acccgccctt ttggaatttc agttaaaaac agaccactgt ttaatattgc
     7381 tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct acttggataa acgaatgctc
     7441 attgaagata tctagactca gagaaaatgg caaatctgct acttcttttt cttttaataa
     7501 ttcatgccca cgttctagat aactatttat ttcttgttca tcttctgtaa aagctgtcat
     7561 tgtttcggta gggatggctt tggctgaatg cttaaggatt tttttctctt catcgatggt
     7621 taaatggact tcaccgacat actgcccgta ctttccagcg gctgctattt ggacatcatt
     7681 gattacgtga ccttctctga ataaatgatg ggtgtgcgat cctaaaataa catcaattgc
     7741 tggtatttct tttgcaattt ggaaatcatc ctgaatcccg agatgactca tcaaaactaa
     7801 aacatccact tgtgaacgta aatcttcgac taattctggc aaaatatcat aaggattccg
     7861 tatgtcccag ccattcggat tataagtaag cggaaatgga gcagtaaatg caattagtcc
     7921 gattttcgtc tggtgtttcg ttgtaatgat tttatatggt ttcgtccatg ccggtggctg
     7981 gagcgtattt ttatcgaata agttatccag gatgatatca aactcagcat gatcgtataa
     8041 atgattcaac tcttctttgg aatttcctac gccttcattg tttcctatcg tcgctgcatc
     8101 ataaccaact tcattcatca gcaagatatt ttcctgtcca ttcgttgctt ctgacaatgg
     8161 atgccaacga tccacaaaat cacctagatc tactgcaatc gttgtcgttt tttctttctt
     8221 ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc cttggccaat tttctaaatg
     8281 agaatgcaga tcattcgtat gtaaaatcac tattttttcc atgtaccctc tcgcctctct
     8341 ttatctttat cataccatag gcagcttttt cttccattga aacaagctct atttcagagt
     8401 ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg ataaatcagt ctggctaaaa
     8461 aatcaggaac attcattttt acagtactat aaaaataaaa aaaacagacc cgaaagtctg
     8521 ttgtcgtttc attataatca gacacatggc ggaacctgct tagtgctgct tccttccaga
     8581 cctgacacgc ttcacaagcc caccattgtc ctagcctttc ggcaaagaat agtatacctt
     8641 atttttcatt cgttgaccag tcttttttta atttcttttt tcgctggcgg agctttcgga
     8701 aaaagtcagt caaaatcatc ccacattcgt cttctaaaat gccaccttct acatatgctt
     8761 ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt accggctgtt ccgccttttg
     8821 gatcatacgc gccgaaataa acttctgcta cacgagacaa aatcatcgcg ccgctgcaca
     8881 taggacaagg ctctaaggta acaaaaagtg cagcatcttc caagcgccag cttcccactt
     8941 tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt agtagcatct tgcgtggttt
     9001 ctctaaggtt atatcctctt ccaatcactt ttccatccaa tacaaccact gctccaatcg
     9061 gaacttcatg aagcatttct gctttctttg cttctgccaa tgcaaggcgc atccattttt
     9121 ctttttctac tgcatcaaga tttgtattta ccaattaccc ttccctgcct ttgttttttc
     9181 ttgttttact ttagcatatg ataaaaaggc tgtaactaac ttttttttct atggtaaaat
     9241 aaatacaaga ggtgaacaag atgaaaatgg ataaattact tgctctttac ccacaagcac
     9301 agaaaaaaaa ctctccagca tcagaagcgg atatcctttc ttttgcaatt gatgatgctt
     9361 ttatctggat caaacaagat tcattatctc aacaagaaac cagccttttg aaagcgttat
     9421 tccctgtaat caacgatcaa aaaaaacacc catggtatca ctatcttttt aaagatgctt
     9481 cttgtgttca agaaaaaagc tttcgtttga tccagcttca cgtcgaatca aaaggagaat
     9541 tcttaaaaaa agaatggcaa gatactattt tggaattatt tcgctattct gctgatttct
     9601 ttttttatac agacacagat gcgctcttag tcgaacaaat aaaccgtgat tatcttgatg
     9661 ctactgaaat caacggtatt tttctatctt tagatgctga ttttgatacg acaacgtctg
     9721 tattcgttgg ttctttccat tcacctggca ccgacctcgt ccctttgttt gctgaagaaa
     9781 gaaaaatttt tttaacagag caaaacaatc tttatactca cagccgtact ttttctatgc
     9841 aagatgtggc tttacattac tatacaaaag atactgtaaa agatagtaag ttgatcaaaa
     9901 gttacataga actgatcaaa caaaatgaaa tggaagtgat cattcaagaa ttatggaatg
     9961 aattaggcaa tgtcagctct gctgcaaaaa cactgtttct tcatcgtaat actctaaaat
    10021 atcgaatcga aaaatttcaa gaacagtctg gtttcaatct aaaaaaagcc aacgatttac
    10081 tgttttgcta tcttttattg cttcatgaac ataattaaag atagattaaa ggggttgtga
    10141 caataatttt gtcacaaccc cttatttcga atgaatagtg ttcaaaagct agtttctctt
    10201 acatcatttt ttcaacaaaa tgcattagcc ggtcttttac atcttcggtt gaatctttga
    10261 ttgaatcacc ataaacagag attttctttt ttcctgattt gatttttgct aacattccca
    10321 tcaaagaatc caagtcacta tctgaaagac catctacgat ttctagtaat ttttcatttc
    10381 catcaaattt gtcatagacg aattttttta ctttcgtccg agtagtcata tgatggatct
    10441 tttccattac ttttcccgat acaacgactg ctgtcgctac actggcagcg gctgctacgc
    10501 ttaatccgat tgttactttt gttgatgttt tcataagtat cagctccttt ttatctatct
    10561 ccatcatagc atagacaaaa aaataaacga actaatccac tcagtgaaat ctcaatcctt
    10621 tagggaaaaa gttctttact gttcctttta cttgtttccc aaatccgaca ggtatcttat
    10681 ctaatactag caaaacccat ccttgattcc catcacgttg aaatgtttct cctgctgtgt
    10741 aagattgcca ttccttttga gtgatcggca agcaaggtat attctctatc ttttttgtag
    10801 caagcgctaa tgcatagcta ggttcaaatc gattcttttt gaaatctcct aaatgcaaac
    10861 ctgtacgtac gacttttaaa ccatcgagag aaggggctaa ctcaggaact tcccaaagat
    10921 ggtcgttgaa cactaaaagt cttccagtag cttcataatg aaaatcattt gaaaactccg
    10981 tccagagttt ttcctgctct tttgtcattt gcactttact tttttttcgt gtcttctttt
    11041 ctttatgcat ctgattttgt ccatgaaaag tcagctttgc aacaaaatgt ccttcaccct
    11101 gatctttatg aggccatata cgtattgttt tttccaaacc agctacgctt ccccattcag
    11161 aacgtcctga actaacggat tgagtcaaag gaatctcctc aatcgttact ggataatttt
    11221 ctacaagcca gctaatgatc tcttcatttt cttctggtgc aaacgtacaa gtcgaataaa
    11281 tcagttgtcc tttatttttt agcattttta ttgctgaaga caaaatctct tgctgacgtt
    11341 tttgacagta tagcggagat tcttctgtcc actcttttat tgcatttgga tcttttcgga
    11401 acattccttc tcctgaacaa ggagcatcaa ccacgatccg atcaaaaaag cccgaaaaat
    11461 gagggacaag ttctgcgggt gcatgatttg tcactattgc gttcgaaact ccccatcgtt
    11521 ctatattttc tgataaaatc ttcgcacgtt tcggaaagat ctcattcgtc actaataacc
    11581 ctttcccctt catttgagca gctaattggg ttgatttacc accgggagcc gcacacaaat
    11641 ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc aacgatcatt gcacttggtt
    11701 cttggctata ttcataccca gcttgatgga ggaaagactt tccattgaca gtgccaagaa
    11761 atccctcatt gctgtaggga gcgggttgta attcttcgct atggtaagtt tggaccatgt
    11821 ccaatcctgc cggttttagt ggattccacc gaaatccttt tttcacgctt ccttgttcaa
    11881 gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag acgatatttt ttgataaatt
    11941 gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt tcgttagaca ttttacaatc
    12001 ttttttacga aaaataaagg tggctgatct actttttcat gttgctgcct aaggtgactg
    12061 ttcctttaag atattttcca agtattcatc cgtcacctcg tttttatccc aaacagcgtt
    12121 catcatttca agaaaatctt gagaagaatc atctgagcgc aaacttccca atgcattaag
    12181 ccataccaat tcaagcgact tttcttcttt gatcttcgtg tgacctttga catatgagaa
    12241 aaatggccgc ttaaatagcg aatccatgtc taaatcagat tttgcaatga tttcacgaat
    12301 actttcatgg atagaaaaaa aatctttcaa taagtagaca atgcttaaaa catcatcgga
    12361 actgcattca gctaagtagt tttcaaaata aacagtgatt tcttctaata tcttctcttt
    12421 taacccgctg attgatttga aatgtgtata aatcgggtat gtagaacaac cgcagaactc
    12481 tgctaactct cttgcagtaa ttgacttcat tccatattct tccacgtata ccatactttt
    12541 gaacagcaaa tattctttac tataatttgt tactctcccc atcattctta cctccgtctc
    12601 ttttcatcta cccataaata aataacataa ataattctaa aaatattatt gataattatc
    12661 aaaacatatt tagttatatc cattaaaaat caatt
//
LOCUS       DACDRK010000074        12308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDRK010000074 DACDRK010000000
VERSION     DACDRK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.074"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            288..695
                     /locus_tag="IWJ77_002209"
     CDS             288..695
                     /locus_tag="IWJ77_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453788.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="IWJ77_002210"
     CDS             709..1110
                     /locus_tag="IWJ77_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453789.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="IWJ77_002211"
     CDS             1112..1483
                     /locus_tag="IWJ77_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453790.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1818)
                     /locus_tag="IWJ77_002212"
     CDS             complement(1519..1818)
                     /locus_tag="IWJ77_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453791.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1824..2054)
                     /locus_tag="IWJ77_002213"
     CDS             complement(1824..2054)
                     /locus_tag="IWJ77_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453792.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            2671..3333
                     /locus_tag="IWJ77_002214"
     CDS             2671..3333
                     /locus_tag="IWJ77_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453793.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3412..6637
                     /gene="fss3"
                     /locus_tag="IWJ77_002215"
                     /pseudo
     CDS             3412..6637
                     /gene="fss3"
                     /locus_tag="IWJ77_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            6751..7065
                     /locus_tag="IWJ77_002216"
     CDS             6751..7065
                     /locus_tag="IWJ77_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9453794.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            7078..7452
                     /locus_tag="IWJ77_002217"
     CDS             7078..7452
                     /locus_tag="IWJ77_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9453795.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            7462..7806
                     /locus_tag="IWJ77_002218"
     CDS             7462..7806
                     /locus_tag="IWJ77_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9453796.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            7877..9229
                     /locus_tag="IWJ77_002219"
     CDS             7877..9229
                     /locus_tag="IWJ77_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9453797.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            9289..10431
                     /locus_tag="IWJ77_002220"
     CDS             9289..10431
                     /locus_tag="IWJ77_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP9453798.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            10456..10710
                     /locus_tag="IWJ77_002221"
     CDS             10456..10710
                     /locus_tag="IWJ77_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453799.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            10966..12150
                     /locus_tag="IWJ77_002222"
     CDS             10966..12150
                     /locus_tag="IWJ77_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9453800.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            12165..12284
                     /locus_tag="IWJ77_002223"
     CDS             12165..12284
                     /locus_tag="IWJ77_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9453801.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
BASE COUNT         4168 a         2017 c         2646 g         3477 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt
     1741 ccatacttta taaaaatctt tgataaatct attcgtagga tcgaatttaa accataattg
     1801 tgtctcatta aaaagcataa ccattactcc gatctgtttt taaaattagt ttctacctct
     1861 aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca
     1921 ggttgtttat tttcggaaaa agctaatata gctaaaattg aaacatccat ctggaattta
     1981 tcttttttgc tgctttgctc ataaaagtct gcatattcat cactgatatt ttttctaata
     2041 aagtcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt ctcgttgtaa
     2101 agcgatattt gaggggcaaa aagggggcaa aaatacgttt atctttgata tatctcagat
     2161 tgaacatagt gtttattata tgtcggtatt gttgatttat agctgttttg agggtattag
     2221 atacataata gaatatagtg gttttgttta cgtgcattac acggaaaagc tgctcgtatc
     2281 aaagaaatca gaagataaac acggaaaact tccttggaaa tctgaaattt gtgaaaagtc
     2341 cttgtatatc aagggctttt tcttttttct ctatcacagt tttaagccat ttttggctgt
     2401 tttggaccat ttttcaatca tattccctta aaaattgtcc tagattgtcc gacattgact
     2461 atcagtgtcg tcaaaatagg gcaaaattta gtagtcagct tgacgacaaa tataatacga
     2521 aaaataccaa aacatttacc gaatttagca acttttgaac ccaagtttct caaagggtca
     2581 gctattagca aaaaagacaa aacatgtact tttctactaa aaagttgatg tgataggctt
     2641 ctatctaaat aactattagg agtttttatc gtgaagcaaa atgatggacg tatcatttgg
     2701 aaaaatcaaa gtgagttaaa actgatttta acaattaatg aattcatcga aaaacacgga
     2761 attacatcta gccgtcaata ccagaaaaag ctatcagaaa atccaaattc tgcaccaagt
     2821 atgtggttca ttaacaaaaa atatggatca tgggagaatt tattgattag tataggacga
     2881 gaaaatactg ggtatggaaa atgggctaga atgtcagaac aagaattatt ggaaatagta
     2941 gaagccttta ttaagtgcga gaagataacc tctcaacgaa tgtacgaaca aaaatcagta
     3001 gggaagaata ttccttcact tagtaccatt aaaaagatgt taggagatat acgtccactt
     3061 tttaaagaaa aaaatgatgg atctcgattt actgatttcg aactgctgtt agaactaaaa
     3121 aatgaaataa tccgattaaa actacaggat gatttatcaa tgacaaagtt tcgaaaacta
     3181 gttcaatccc caaaactacc atcagttgat acaattatga aaagaacgaa caaaaattgg
     3241 gaggaattga tggcagagat tggatttgat tatcgaagaa tcaaaattta taagcagaga
     3301 aacaacctat ccaagacaaa gaaaactaaa tagtatcact ctaaatcaga cgttcaaaat
     3361 taaaatttgg acgtcttttt tattacccaa aaacgaaagg agatttattt tatgaaaaag
     3421 ccaagattta agaattggcg tttgctcgca acactgagtc tattatgcca aacaattggt
     3481 ggatcgcttg gcccaacgat tgcctttgct gatgaaatta ctcatccaca aacggtaact
     3541 gtggaattgg atttggctca ccagtatgct gtcgaaggta catttagcga tggtcgtccc
     3601 atgtcagaag ttaccgttcc gcactatgca gtttacaatg gtgtgaagca agatattttc
     3661 tgtattgagc caggggtgcc gatttacaat gagttcactc ctggttatga gaaaaatcca
     3721 ttgcctgata tgtcggagaa agcgaaatta gtttccgttt tatggaagga agctggtacg
     3781 gatgtggata cacatattgt ggcccagaag atgatttggc aagaagttaa tggctacgct
     3841 tcactcaatt aaacgttctg atggtagtgc agtaaatatc gcagccattg agtcaaaaat
     3901 caatcaagcc attgcggatt atcaaaagaa accaagcttc cataactcca cagtgaaaac
     3961 cgtgttaggt caatcgacta ctgtgacaga tacgaacggc ttgaatttat cggagtttga
     4021 tgaagttgtc gagaatatcg caaatattga ttatcgtgtg aacggcaatc aactagtgat
     4081 tactccaaat gccaattcaa aagagagtgg tgaattaacc ctaaagaagt cagctaatac
     4141 aggaacaccg gtagcttata aaatggctgg tcaacaaact ttgatggcgg gggcaattga
     4201 taagcccaat acctacacaa tcaaaattga tgtggaaact gagggttctt tgaagattaa
     4261 aaaagtcgat aaagaaacag gaaatgttgt accgggaacc gttttccatt tagactttgg
     4321 aaaaacatta cctgcaaaag acgtgaccac cgataaagaa ggcattgcga cattggatga
     4381 gattcctcat ggcgcgaaag taaccattac tgaaaaatca gtgccagctc cttatacgat
     4441 tgacactaca ccaatcaccg caaccattaa atctggtgag actatttctg tgacttcaaa
     4501 aaatactcgc gaaaaaggtc aaatcatttt gaataaaatg ggggtggaaa caggaactga
     4561 tctttggaac gacaaatact cattagccgg aaatacgttt gccattcgca aagacagtcc
     4621 aaccggtgaa attgtccaag aaatgaccac ggatgaaaaa ggtcatgcgg aaacaccaaa
     4681 agagattgcc aatgcgttgg aattgggaac ctattatgtg accgaaacca aagctagtaa
     4741 tggctttgtg aataccttta aaccagtaaa agtcgaattg aactatgtca atcaaaccgt
     4801 ggctcttgtt accagtaacg taaaagggca aaaccaagaa atcactggtg aaaccacttt
     4861 gacaaaagaa gacaaagata ccggtaatga aactcaaggt aaagctgagt ttaaaggagc
     4921 tgaatatact ctctttactg caaaagatgg tcaagcggtc aaatggagtg aggcttttaa
     4981 accagagtta gtgaagggaa cgaaagcttc tgatgaaacg gtgactttga ctttagatga
     5041 aaagaatcaa gttgctgtta aacacttagc gattaatgag tattactggc aagaaaccaa
     5101 agcacctgaa ggatatacct tggatgaaac aaagtaccct gtttccatca aaaaggtgga
     5161 tgataacgaa aaaaatgccg tgattacccg agatgttacg gcaaaagaac aagtcattcg
     5221 ctttggcttt gatttcttta aatttgctgg atcagctgct gggactgccg aaactggctt
     5281 caatgatctg acctttaaag tatcgccatt ggaaggaacc agtgagatta caggtgccga
     5341 agatgaagca attacagctt ataacgagca actaggtttt gatggctatg gcaagtttga
     5401 aaatcttcct tatggggatt atttacttga agaagtagag gctccagaag gatttcaaaa
     5461 aattacgcca ttagaaatcc gttctacttt taaagaaaat aaagcggatt acactaagag
     5521 tgaatacatt tttacgatta ccgaaaaagg acaaaaacaa ccaattaaaa cggtgaccgt
     5581 tccttacgag aaactgacga ataaagcatt ttctgttagc ttgaatcgtt tgatgctcta
     5641 tgatttacct gaagaagaag atagcttaac ttctcttggg acttggaaag acggaggcaa
     5701 agaattgaca gaccttgatt ctaccgagct agttgataaa ttgagctata acttgcatga
     5761 aatcaaagaa gactggtatg tggtagcgca agccattgat gtggaagcga caaaagctgc
     5821 ccaagaaaaa gatgaaaaag ccaaaccagt ggtaattgcc gaaacaaccg caacgttggc
     5881 gaataaagag aaaacaggaa cttggaaaat tttgcataaa ttaaccgcag aacaagtttt
     5941 ggataaaacc atcgtcttat tcaactatgt gtatgaaaac aagaaaccct atgaagcagg
     6001 tgatgatcca gtagcgaaag atgctagctt gaacaatcaa gcccaaactg tcaattgtac
     6061 ggtggaacgc catgtttcca tccaaacaaa agcccaccta gaagatggtt cgcaaacctt
     6121 tactcatggg gatgtggtgg atatgttcga tgatgtgtcg attacccatg atgtactgga
     6181 tggctcaaaa gaagcttttg aaacaattct gtatgcatta cttccggata atacgaccaa
     6241 agaaatttgg aaatctggca aaattgatta tgaagtgaat gacaaagaat tcactaaaac
     6301 cgtacttgcg gaaaaagtgg acacaagtaa atatccagaa ggtacaagtt ttaccttcaa
     6361 agaaattaac tatggcaaag acggaaacat caatgggaaa cacaatgagg atctaaaaga
     6421 aaaaactcaa actttaacac ctaaaaaagt tccgacaaca ccaagtgcac cagaacaacc
     6481 ggaaatacca actgttccaa gtgactctca agaatctagt cctacagtaa agacattccc
     6541 acaaacaggg gagaaaaatt ctaatgttct gctgtttatt gggtttacct tgatctttgc
     6601 gactgctggg tattatttct ggaatcgccg aaactaaggt gatgtgatga aacaactaaa
     6661 taggaaaaac caacgtgcgc gaccgcctcc taaaaaaagt ggtcgcgcta tttttgtatc
     6721 caaaactaaa aatgaaaaga ggaaaagaag atggaattga aatttgttgt gccagatatg
     6781 gccgaaacct ttggaaagat aaggtatgcc ggcgaaggcg aagttttaac tgaaggatat
     6841 gggcggaata ctacagtaat tggtcgtagt tatcatttgt attccagtaa gcaacgagcc
     6901 gatgatattg aagtggtggt agctgcggaa gccggcgaaa aggattttga tcaagatcaa
     6961 ccactaaaag ccgtgaatcc ccatttggtt gccaaaggct atgagattga gaatcgtggg
     7021 tttaccgact acgtgttgta tgtcgatgat ttagtgaaag cataggagga aaagaagatg
     7081 agattaacag aaggtattgt tgtagattca ggactaacgt ttgggaaatt acgattttcc
     7141 gcattacgcc gagaagtacg gaagcaaaat gaggatggaa cggttagtaa cgaggtaaaa
     7201 gaacggactt ataatttgaa atcctctgcg caaggtcgaa tgatccaagt tagtatccca
     7261 gcaaatgtac ccttacgcga gtttgcctat gacgcagagg tagagttggt caatccaatc
     7321 atggatacgg tcgccaatta tgttttccga gaaggaacta ctgtcaattg gttcattaag
     7381 gccgatgatt tagtcttgaa acgacaacca gatcagggaa attctacaaa ccaaaatgaa
     7441 ggaaagaaat aggtgaagcg tatggaaatt aatgttgaac atattttaga ttgtctcgat
     7501 caatatggaa aaggtgagct aacggaggaa caactcacta aagcattaac ctatgatgaa
     7561 aaaatgtttc tgattatgca tcaagggtta ctggaagtag gtaacgatgc cgaagaagat
     7621 tttgatgtac tgaattggtt aggggaagaa gagtccttct ttatggttat cgaagtaaat
     7681 gaaagtctat gtcgagaagc cgaaaccgta ttggaagaaa ttggtgtgga aatgccggat
     7741 gccattgaag tttttttaaa gcaactagtg gaaacgaaac aactccctat agcagtaaat
     7801 gactagcata actgcttgaa attggatcaa tgaaatccta tgaagctttt ttcgattgga
     7861 ggtggaaacc ataaaaatgt acaaagggca tcgtatacgg gcaggagatc agcacttggt
     7921 ttatcacttt gttcttggct ggctactcgc tctgttcatc ggttggatga gcgtctttta
     7981 ttttcaagag cttagacaat ttgatatttc taagctatca ctttctacta tagaaatcgt
     8041 tcgttctata aaagatttaa tctatttgct aggaagtctc gttctttcag gaagcacgat
     8101 gttgctttat atacactttt ttcaggatca ttggcgcagt ttgtggcatc gacaaaagct
     8161 agcccggatg attctagaaa atcattggta cgaagtaaaa caaactcaga gtgaaggatt
     8221 tttcaaagac ctcaatagta gtcgaaccaa agagacaatc agttacttcc ccaaaatcta
     8281 ttaccgtatg aaggatggtt tactttctat tcgcgttcaa atttcactgg gaaaatatca
     8341 agatcagctt ttaaagctgg aaaagaagtt agaaagtggt ctatattgtg aactagtaga
     8401 aaaggaactc aaagactcct atgtggagta cactttactg tatgatataa ttgccaatcg
     8461 aattgggata gacgaagtag tggcagaaag tggggctttg cgattgatga aaaatcaagt
     8521 atgggcttat gattctttac ctcatatgtt aatcgctggc ggcacaggtg gtgggaagac
     8581 ttacttttta ctcactatca tcgaggcttt attaaaatct gatgccgagc tttttgttct
     8641 tgatcccaaa aatgcggact tagccgattt aggtacggta atgcctcatg tctattccca
     8701 aaaggaggaa atttctgctt gtgtggaaga tttctatgag cgcatgatga ctcgtagcaa
     8761 ggcaatgaaa gaaatgccca actacaaaac aggagagaat tatgcgtttc ttggacttcc
     8821 accaaacttt ttaatctttg atgaatacgt ggtttatatg gaaatgttaa cgacgaagga
     8881 aagtgcggtg attttgaata agctcaaaca aatcgtgatg ttaggtcgtc aatctggttt
     8941 ctttctgatt ctagcttgtc aaagaccgga tgcgaaatat ttaggagacg ggattcgcga
     9001 tcaatttaat ttccgggtgg ctttgggtcg tatgagtgaa ctgggatatt ccatgatgtt
     9061 tggcgaggtt gataaaaaat tctttatgaa gcgtatcaaa ggtcgaggct atgtagatac
     9121 gggagggagt gtgattagtg agttttatac gccacttgta ccaaaaggat atgatttctt
     9181 aagggaaatt agcgacattg gtggattaaa cgttaatacg gaaggataaa ataatagtat
     9241 gtagcctagg agtgtcaaga atcctgggct ttttttacga ggtgatttat gacattttta
     9301 gatttattcg ctgggattgg tggcttttgc ttaggtatgg aacaagcggg tcaccagtgt
     9361 atcggctttt gtgaaattga tgattttgct cgccagagtt ataaggcaat ccatgataca
     9421 agtaaggagg tggaaatgca tgacatcaca agtgtatcag acgagtttat tcaatctctc
     9481 ggaccagtgg atatcctttg cggtggattt ccgtgccaag ctttttcaat tgcgggaaag
     9541 cggcaaggat tttccgatac tcgaggtact ttattctttg aaatcgctcg gttcgccgct
     9601 attctccaac ctaagttttt attccttgag aacgtccggg gattactcaa tcacgaagga
     9661 ggggctacgt tcgagacgat cctccgaacg ctggatggat tggggtatga tgtggaatgg
     9721 caagtgctta actcaaaggc ctacgttccc caaaaccgtg agcggatctt ccttatcgga
     9781 cattctcgag acgcatgtac cgaacaagta tttcctatca ttggatcgtc tccaacatct
     9841 gatcaaaaca tcagaaactt attaaatatc aacccttcca atcgaggaat ggggggacaa
     9901 gtctatgggt cagatgacgt agcgcctacc ttgaccggtg atgaaggaat caaaatcgca
     9961 ttgccggtag atgatggaat ttctgtagcg ggtatgttac tgggaaattt tgaacaaggc
    10021 aatcgggttt atgagataac tggaacagcg cctactttgt caacgaaaca aggcggaacg
    10081 aagatcatgc ttcggaaaaa cgaaacagaa tatcaagagg tgaagccagg gaacagtgtg
    10141 aacctggctt ttcctaattc cacaagtcgt cgaggccgtc tgggaaaaca aagtgtgcat
    10201 accttattaa caggagacca acaagcagtc gtcacggatc agtatcaaat tcgaaagtta
    10261 acacctaggg agtgctggcg gcttcagggc tttccggact gggcttttga tcgagcttct
    10321 caagtcaatt cagatagtca gttgtataag caggctggaa attctgtaac agttccggtc
    10381 atttttgata ttgctagaag attaaaggag gtaaaaacag atgatttatg atgagttgat
    10441 tggggaatct actgggtgat tggcaagatt cagtcagatc cggaattgga agaagaactg
    10501 taccggctca attttgatat tcgcaaaaac ggggtgaaag tccctgatga tccgtatgta
    10561 attgatgagg aaacggatgc tcgaatggag ctgaatcaag tcatcagtga attggaacga
    10621 attgcggact ttgctaaaga acctgatatc cgccaatact tgtttgagat aaaagcagag
    10681 ttggaaattg agggtattac cgcggagtaa cggccaaata aaccactgat aggcactgac
    10741 cgcgggggat ttgcgaagca gaaatcctcc gtggtgctgt gcctgtttat ggacggcgtt
    10801 agccggccat gatgtctcac cccccgtatc taacaggggg gtacaaaaac acaaaaaata
    10861 agcaagcaac gataaggaat ggctgtccta tcaaggataa taagcaattt gtgtagatgt
    10921 taactgattt gaaaaagtta acaccttaaa tttggaggtg agaacttggc acaaaggaat
    10981 ttagattatc ggctattaaa agaccggcgt aatgaatatg gcgtttcaca aaataaatta
    11041 gctacgactt gtggacttag tcgcccgtac ttaaatcaga tagaaaatgg tggcgtaacc
    11101 gcatcgacaa aaaccatgag gaaaattttt gaccaactgg aaagtttcaa tcccgagtta
    11161 cctttaacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga tgcgaggaaa
    11221 atcattcaag agattctcca tttgaaattt gattatatgc tccacgaaga ttacgccttt
    11281 tactcctatc aagaacaata tgttatggga gatattgtgg tgatgttgtc acatgaagaa
    11341 gataaaggcg tccttttaga attaaagggt cgtggttgtc ggcagtttga aactttttta
    11401 ctcgcccaaa agcgtagctg gtatgacttt ttcgaagatt gtctaaaagc cggtggcgtg
    11461 atgaaacgct tagacctggc aatcaatgat cgagttggcc tgttagatat tcctgattta
    11521 acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtaccttcaa aagctatcgt
    11581 tccggggaac tgttgaaacc tgatgataaa gatggtatgg gcaatacttt gtatattggc
    11641 agtcttaaga gtgaggtcta tttctgtttg tacgaaaaag actatgagca atatatcaag
    11701 ttagggattc ctttagacca gaccgaaacg aaaaatcgtt ttgaaatccg actgaaaaat
    11761 gatcgagctt atcatgcaat tcaagatttg ttaaaaggtc gcagtattga aagtaccaca
    11821 ttttccatta ttaaccggta cttgcgattt gcggataagg tggaaggcaa aaggcgaact
    11881 aactggccct tgaatgaaca atgggggcgc ttcattggac ggaatcagaa ggaaattcaa
    11941 ttaacttctg aaccgaagcc ttataccatt gaacgaacgt tgaactggct tggacgacaa
    12001 gtcgccccta cgtggaagat ggccaaggag ttggatcggt tgaatcagac aacgtacatt
    12061 caggatatgg taaataatac tcgactatca gaccggcaca aaaagatttt ggagcaacaa
    12121 agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtgatggaa attgtgaaag
    12181 atattttatt ggtgtttggt ggcagtatgc ttggtgttac tgtgatgtgc ttgatgcatg
    12241 caagtgcagc ggcggaccga gcaatggaaa agaaaggaaa gtgatgaaat gaatttcggt
    12301 caaaattt
//
LOCUS       DACDRK010000075        12148 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDRK010000075 DACDRK010000000
VERSION     DACDRK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.075"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(30..587)
                     /gene="frr"
                     /locus_tag="IWJ77_002224"
     CDS             complement(30..587)
                     /gene="frr"
                     /locus_tag="IWJ77_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9453802.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(590..1312)
                     /locus_tag="IWJ77_002225"
     CDS             complement(590..1312)
                     /locus_tag="IWJ77_002225"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9453803.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1448..2329)
                     /locus_tag="IWJ77_002226"
     CDS             complement(1448..2329)
                     /locus_tag="IWJ77_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9453804.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2428..3210)
                     /gene="rpsB"
                     /locus_tag="IWJ77_002227"
     CDS             complement(2428..3210)
                     /gene="rpsB"
                     /locus_tag="IWJ77_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9453805.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3569..4048
                     /locus_tag="IWJ77_002228"
     CDS             3569..4048
                     /locus_tag="IWJ77_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9453806.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4265..5356
                     /gene="phnW"
                     /locus_tag="IWJ77_002229"
     CDS             4265..5356
                     /gene="phnW"
                     /locus_tag="IWJ77_002229"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9453807.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5349..5477
                     /locus_tag="IWJ77_002230"
     CDS             5349..5477
                     /locus_tag="IWJ77_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9453808.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5481..6026
                     /locus_tag="IWJ77_002231"
     CDS             5481..6026
                     /locus_tag="IWJ77_002231"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9453809.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6480..8171)
                     /locus_tag="IWJ77_002232"
     CDS             complement(6480..8171)
                     /locus_tag="IWJ77_002232"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9453810.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8591..9538)
                     /gene="arcC"
                     /locus_tag="IWJ77_002233"
     CDS             complement(8591..9538)
                     /gene="arcC"
                     /locus_tag="IWJ77_002233"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9453811.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9653..10672)
                     /gene="argF"
                     /locus_tag="IWJ77_002234"
     CDS             complement(9653..10672)
                     /gene="argF"
                     /locus_tag="IWJ77_002234"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9453812.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10763..11992)
                     /gene="arcA"
                     /locus_tag="IWJ77_002235"
     CDS             complement(10763..11992)
                     /gene="arcA"
                     /locus_tag="IWJ77_002235"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9453813.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3314 a         2526 c         2196 g         4112 t
ORIGIN      
        1 tgatccgccc ctctgttttt atgattttat taaacttcca ataattcttt ttctttatca
       61 gcagcgatct tatctacttc tttgatgctg ttatctgtca atgtttgtac gtctttttct
      121 aatccgcgca aatcatcttc ggtaatatca ccattttttt gttgtttttt atattcatcg
      181 atcgcatcac gacggatgtt acgaatcgcg atttttgcat tttctgcttc ttttttaaca
      241 tctttagcta attcttttct gcgttcttct gtcagttgcg ggataactaa gcgaatgaca
      301 tttccatcat tggttggact gattccgata tcacttgcca taatagcttt ttcaacatct
      361 tgaagaatgc tcttgtcaaa aggtgtgatc attaaaacac gtgcttctgg aatttggatt
      421 gatgccattt ggttcaatgg tgttggcgca ccgtagtagt tcacagtaat acggtccaaa
      481 aggctagcgt tcgcacggcc tgcgcggatt tgcccaagtt cacgttgaag attttgtgct
      541 gcttttttca ttttttcttt tgcttctgtc ataattgcat ctgccatttt tatttccccc
      601 ttacagttgt tccgatattt tcgcctaaga ttgcacggcg aatatttccg tgttcattta
      661 aattgaagac aagcagtgga atatcattat ccatgcttag tgaacttgca gttgaatcca
      721 tgacttgtaa tcctttggca ataacttcca gatgagtcag ttcttcaaat tttactgcat
      781 ttgcatctac tttcggatca gcagaataaa caccgtctac attgttttta gccatcagga
      841 taacatctgc gccgatttct gccgcgcgta atgctgctgt tgtgtctgtc gagaagtacg
      901 ggttgccggt accgcctgca aagataacga tccgtccttt ttctaaatga cgttctgctt
      961 tacggcgaat atatggctct gcgatttgac gcatttcaat cgaagtttgc acgcgtgtcg
     1021 gtacacccac gttttccaac gtatcctgta aagctaaggc attcataact gtagctaaca
     1081 tacccatgta atcagcttga gcacgttcca tgcccatttg tgctccgatt tgtccgcgcc
     1141 agatatttcc gccgcccaca acgattgcca tttcaacgcc cagctcgtga acttctttga
     1201 tttcttggat gatttctttg attactggtg gtttgattcc aaatcctgcg tccccagcta
     1261 aagcttctcc actcaatttt aagactacac gctgatattt cggtgtgacc atgttcattc
     1321 ctccaatatt ttctattgcc attttaccat acttagatga tttcgacact ctaagactgg
     1381 tttttcttgt tttgcttaaa aaaagggagc gcacaaaccg tgcgttcccg tctgttattc
     1441 aacagaatta ttttttcact tggctcataa cttcttctac aaagttatct tcgcgttttt
     1501 cgataccttc gccaacttcg aaacgtacga atgttttaac agttgcgcct tttgatgcaa
     1561 cgtatttttc aacagtcata tcaggatctt taacgaatgg ttggtccacc aaagcgattt
     1621 ctgctttgaa tttcttcaag cgtccttcta ccattttttc aacgatattc gctggtttgc
     1681 cttcgttcaa tgcttgttct gtaagaacag ttttttcatg ttctaattca gcttctggaa
     1741 tttgtgattc gtttacataa cgaggattga tagctgctac gtgcatagct acgtctcttg
     1801 caactgtttc gtcagttgtt ccatctagaa cagtcaatac ggcaatacgt ccgcccatat
     1861 gcaagtatcc accaaatgca gcattgtcgt ctttttctac aacttcaaag cgacggaagc
     1921 tgattttttc tccgataact tgagttgctt cgatcaagtc agattcaatt gtacctttat
     1981 ctgttttgat tttcattgca gcttccatgt ctgctggttt gttttcagca acaagttctg
     2041 caatttcttt tactaaatct tggaacattt cgtttttaga aacaaagtct gtttctgagt
     2101 ttacttcaac gattgctgca gtgttacctt tcacagcaac tgaagccaat ccttcagctg
     2161 caatgcggtc atttttcttc gcagcctttg ccattccttt ttcacgtaaa agatcaactg
     2221 ctttttccat gtcgccttct acttcaacta atgctttttt cgcatccatc attcctacgc
     2281 cagtcatttc gcgtagttct tttaccattt tagctgtaac gtctgccatg tttaactcct
     2341 cctgtgtgtt tttcttttta aaaaaagctg cttcaaagga gagtgaggct tgtcgcccag
     2401 cctttgaaac agctccatca ttcaaaatta ttctgctgaa gaattgtcgc cttcaacaac
     2461 atctacgatt tcttcgattg atgttgctgt gttttcttct gtgaaatctt cttcaactac
     2521 ttgatcttca ccttggtttc cttcgatgaa agcatcagcc atttttgaag taatcaattt
     2581 aacggcacga attgcatcgt cgtttgaagg gattactacg tcgatttcat ctggatcgca
     2641 gtttgtatca accatagcta cgattgggat atttaatttg tgtgcttctt gaacagcaat
     2701 acgttcttta cgtgggtcaa cgatgtacat tacatctggg attcttggca tatcagcgat
     2761 accgcctaag aatttttcaa gacgttcacg ttctttgttc aaaccagcaa cttctttttt
     2821 aggtagaact tcaaaagttc catcttcttc cattgcattg atttgtttca aacggctgat
     2881 acgtttttgg atagtatccc agtttgtcaa tgttccgcct aaccaacgat ggttaacgaa
     2941 gtattgtcct gcacgagttg cttcttcttt gattgcttct tgtgcttgtt tttttgtacc
     3001 tacgaataat gctacgccgc cttcttcagc aacgctcttc atgtaatcat aagctgcatc
     3061 tactaattta actgtttttt gcaagtcaat gatgtagatt ccgtttcttt ctgtgaagat
     3121 atatttcttc atttttggat tccagcgacg agtttggtga ccaaagtgta cgccggcttc
     3181 tagtaattgt ttcatagaaa ttactgccat gtttgtttcc tccgatttgg ttttattttt
     3241 cctcttctta tcttctacct tgccggcaaa ctttttcaag cacctaccga caatccaata
     3301 agaatgtgga tttgctgatg cataaagcac cctcagctat tatacagaca aaagatccgt
     3361 atcgcaagag caaatatact tttctgctca acattcaata gtttacctga aagataaaaa
     3421 gaattcaaga aaaacagtag atttctcttt gtttctttat tatccttttt ccataaaata
     3481 attttaggta taaaagacga atattattgg gcaatccttc ttttttttgt tatgatacat
     3541 gtgaaatctt tcgtaaagga gaaagaaaat gcgcaaagct caaagacatt tgctgattaa
     3601 acaagtcatt gaagaattcc cgatccgtac gcaagaagaa ctattaaaca aacttgggca
     3661 ttatggcgtt accgccacgc aggcaacgat ttctcgagat atccgtgatc taaaaatcgt
     3721 gaaggcacct gatgaaaatg gcgtttcccg cttcgtattg tttcgcgaaa aaggaactcc
     3781 tgagactagg actgaggaag aggatcggct ggttcaaatg attgaagaca ttgtattgaa
     3841 agtcgaacga gtccactttt tgacgatcgt caataccttg ccagacaatg ctcatttatt
     3901 tgcatctgtt ttagatgaga tcaaacctcc ctctattgtc agtacaattg ctggattcga
     3961 tacgatcatg gtcatctcgg aaaatgagga ggacgcaaaa cgagtagaag aatttcttca
     4021 ccatccaact gaagtatcac ttttttaatc agagcacgta ttcctaatat ggaatgcgtg
     4081 tttttttatt ttcaatattt tccttattga ataaaccttt acactcttta caaaaataca
     4141 tcatttttta ttcaaatttt tcaacacctt gattgaatca caataatcat catttatatt
     4201 ggatgttaac aagagcagct tcttgctctt tgtgtatttc aaaaaagaaa tgaaaggaaa
     4261 tcatatgtat aaattattaa cttctggacc attgacgact tctgaaacag tgaaaaatgc
     4321 tatgctgatc gatcattgta cttgggatca agaatacaaa gaaattacac aatggataag
     4381 aaaaaaatta ttggatcttg ctcaagtatc ggaaggaata tacacaacgg tcttaatgca
     4441 aggtagcagc tccttcgggg tagaatcggt attaagttct gtgatcaggc cagaagatac
     4501 tgtactgatt tgttcaaacg gcgcatatag acaacgaatg atcaacatgt gcgaatgtct
     4561 gaaactgaac tataccgttt atgaggtatc ggaacatcaa atcctccaag ctcatgtgat
     4621 tacacagctg attgaagaga atccgtctat ctccgcacta gcaatggtcc atagcgagac
     4681 tacttccggt attttgaata atttggaaga gattgctgct gtcacacaag aaagagatct
     4741 tttgtttata gtggacgcga tgtcaagctt tggtgggata ccgatttgtg tagccgaact
     4801 agggattgat tttttgattt ccagctcaaa caaatgtatt caaggtgttc caggcttttc
     4861 ttttgtactt gctaataaag cggaactgga aaaacgaaaa aatgttagcc gaagtttgtc
     4921 acttgatcta tatgaccagt accgcgtgat ggaaaacgag aatggcaaat ggcgtttcac
     4981 ttcccctacc catacggttt tggcttttca acaagcgctg aaggaactag aggcagaagg
     5041 aggaatcgaa actcgatatt cacgttatct cgccaataat catgtgctac gacaaaaaat
     5101 gacagagatg gggttccagc ctttcattca agaatatcag gggccattca tcacctcttt
     5161 tctttaccct aaggactggc gatttgattt taatcatttt tatcagtatt taaagcaaaa
     5221 tggatttgtg atctatcccg ggaaaatcag cgatcatgaa tgttttcgta tcggaaatat
     5281 tggcgagatc tatccggaag atattttgga actgacagca attatcaaag actataagga
     5341 gaacgttaat gattaaaact gtaatttttg attgggcagg aacaactgtg gactttggct
     5401 gtatggctcc tgtccacgct tttagaaatg cttttctaga aaaaggaatc caactgactg
     5461 acaaagaaat ccgctaaccg atgggaaaat taaaatggga tcatatccaa cagcttcttt
     5521 ccctcccttc tgtcaaaaaa caatggatag ctatttatgg acaactacct caggaaaaag
     5581 aggtggagga attgtatcaa caatttgaac attatctgtt taaagatttg gtcaagcatt
     5641 ctaccttgaa acccgacaca ttggaaacga tcgaacggtt gagaaacgct gggatcaata
     5701 ttggttccac gaatggattt aatgctccta tgatgacaat cgtagcagaa accgcaaaaa
     5761 aagaaaggta ttcaccagac ttcatggcaa cttttgaaga cgtcgaaggg tatggtcgac
     5821 cgtttcctta tatgatccat aaaaacatgc agtacttcaa aaacaagtcg gttgatgaag
     5881 taatcaaagt cggagatacg gtctcagata tccaggaagg gaaaaatgcc ggagtgttga
     5941 cagtaggtgt aattgaaggt agttcgctaa tggggatttc ttatgatgag taccaaaaat
     6001 tagagatggg gaaaaagatc gtttaaaaca gtctgttgct cttagattcc aacaagccgg
     6061 cgctgatatt tgtgtaaaca atttaaaaga gttaacagaa tacattttgt acgctgacat
     6121 gaatccagaa tgacttttat acaaaataag aggttgtgaa aaagctgtcc tgtatcaagt
     6181 aataaaaaca gccaagcaaa aatagctcct catatttttc gtttggttag gcttattacc
     6241 gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa aagggagctg tgacacgact
     6301 tttgtcacag tccccttatt tgcaaacaaa cagtgtttaa gagacaacct ctccactaaa
     6361 cggtgttcat tcagctgact ctcggtattt gctcaaaaaa cgataaaatt tgaaaagcta
     6421 ttttctcttt tgtttctcta atactcggat cagaacgctg atttcacatt ctctttttct
     6481 tacatcttat ctggtgcatg aagtcctagc aaacgaaggt cttctttcaa taatacagtc
     6541 actgcataaa ctagagctaa gcgtgcttct ttttgttcat catcagctaa aatcttcgta
     6601 tgagcatagt atttgttgaa tgcttgtgct aatttgattg catgtttcgc aatcactgac
     6661 ggttcgtatt tttccccagc agacaagacc gtttcagggt atttttgaac taatttaacc
     6721 acttcccagc tggtatcgtc attcaatgca tagtctgctg tttctgaagg gacaaatcct
     6781 gctttttcca acaggctgac tgcacgagca tgtgtatatt gcacataagg tccagtttct
     6841 ccttcaaatc gaacaacttc ttccaggtta aagtcaaatg tattcagtcg atcgtttttc
     6901 aaatcgtgga aaatgactgc cccaacccca acttgtttag ctacagcatc tttgttttcc
     6961 aaatcagggt ttttctcgct gatttgttct ttagcagatt ggattgcttc atttaatact
     7021 tcttctaata agacgatctt tcctttacga gttgataatt ttttaccacc ttgcgtgatc
     7081 aaaccaaatg gaatatggtg catgtcgtca gaccagtcaa agcccatttc cttcaagaca
     7141 gctttcaact gtttaaaatg atagctttgt tcattcccta ctacatatag agattgtttg
     7201 aagtcgtaag tacgtttacg gtacaatgca gcagctaagt cacgtgtaat gtatagtgtc
     7261 gcaccgtctg acttcttaat caacgctgga ttcaggtcat aagcagatag gtcaacgatc
     7321 tctgcacctt tatcctcgtt cagcaaatgt ttttcttcta aaagtttgac gatttcatcc
     7381 atcttgtcat tgtagaaagc ttcaccgttc aacgaatcga aacgtacttc cagcagatca
     7441 tagattttat tgaattcttt catcgattca tcacggaacc attgccataa ttgaatagcc
     7501 tctttatcgc cttcttccag acgtttgaac caagcacgag cttcttcatt taattctggt
     7561 tctgtttcag ctacttcatg gaattggacg tataaacgaa gcaattcatt gattggttct
     7621 gctttaacag cttcttctgt accccatttt ttgtaagcga cgatcagttt tccaaactgt
     7681 gttccccagt cacctaaatg gttgatgcgg attggctgat aaccgatttt ttccataatg
     7741 aaaccgatcg aattaccgat aactgtcgaa cgcaaatgtc ccattgagat tggtttggca
     7801 atattaggag aagacatgtc gattggtact gttccttgat tcccgatgtt gctatcccca
     7861 taatgttctt tttctttcac tactgtttgc aaaacttttt tgctgataag ttctttattc
     7921 atgaaaaagt tcaagtaagg tccgacaact tcgatttttt caaagttggc agaatcgatt
     7981 ttttctgcta aatctgcagc gatttgctgt ggcgcttttc ggtaaacttt tgctaatgaa
     8041 aaggctggaa atgcaacatc tccgtgttca gctgattttg gattttctag taattgctcg
     8101 acttgttcca gtgttaaatc atctttgaca gcgtcataga cagcttttgc gacaagatct
     8161 ttattattca tctttattcc tcctaaagtg ttcccttgat tgttaaacaa aaaaaatctc
     8221 tagcgacggc atacttagta tgccatcact agagacgaga tttcccgcgg taccactcca
     8281 attgtcctaa aaaaggacca gctccatctg atatcggtag acgaccgtct gtttaaaaca
     8341 gaattctcca agtgcgcttc gttgtatctt cttcattcgg cttccaccac ttccgaattc
     8401 gctagccaag aatgatacaa gtactctctt gttcaacaaa tcttagtctt attaaacttt
     8461 cttgaattat atcagaaaaa aaaacgaaag gctagtcgtt tcataaaaaa acaatccaga
     8521 tggcctaaag gagaagaaat accacctgga tagctataaa tcatttcgta aatacgaaag
     8581 acttaacaaa ttaatcagct acgataatcg ttccttcttc agaagctaaa agattctcga
     8641 tattttctaa agaagtgatg attgccttag cgtttggttt tgcttcaaca aatgcaatag
     8701 ctgcttctac tttcggaagc atgcttcctg gagcgaattg cttttcatca atgtattgtt
     8761 tcatttctga aacagtaaca gtttctaatt tcttttgatc tggtttttgg taattgacat
     8821 acacattgtc tactcctgtc aatacgatca gcaaatccgc atcgatgatt tcggctaatt
     8881 tttctgaagc aaaatcttta tcgattacgg cttctcgtcc ttctaatcca gtagctgttt
     8941 ctacaacagg aatcccaccg ccaccaacag agactgtgat aactccttga tcgaccagcg
     9001 tttcgatgac acgtgcttct ttaatcgaga tgggtttagg ggaagcgact acttttctcc
     9061 atccacgtcc agcatcttct ttgaatgtca cagtggagtc agcattcatt tgttctttgg
     9121 cttcttcttc cgtgtagaat ggaccgactg gtttacttgg atttttgaat gaaggatcat
     9181 tttcatccac aataacttga gtgactaaag aaaccacatc tttatcgatc cctttttcct
     9241 tcagtacttc gcccatggcg ttttgtaacc agtaaccgat agatccttct gtcattgcaa
     9301 cacatgtatc taaaggcatt gccggtgttt tctcagaatc agcagcttgc tgctgaatca
     9361 ataggttacc gacttgcgga ccatttccgt gggagatgat caattcatct ccttgttcga
     9421 taaatttcac taaatacttt gctgtttcca ttaacgcttc ttgttgggct tttgcacttg
     9481 catccgtaga caagatggca ttgcccccta aagcgacaac tacttttcgt ttggccattt
     9541 cattcgtcct ttcttcagtt ctaccgaatt ttctagataa caaaacgagc cagcgttttg
     9601 tgattttccc tcaatgctga ctcgtttttt cgtgagatga atgtggttat gcttaaacgc
     9661 gagggataaa caagttacct aatgttgctg ccatgatcgc tttgattgag tgcatacgat
     9721 tttctgcttg gtcaaattga cgagcatatt tgctgcggaa gacttcatct gttacttcca
     9781 tttcagtgat gccaaagcgt tctttcattt gttcaccgta aacagtgttt gtatcatgga
     9841 aagctggcaa gcagtgcaag aagatcaaac gatctgtgtt gtgtgttttt tctaccattt
     9901 ccatattgat ttggtaaggt ttcaacaatt tgatacgttc ttcgaattta tcttcttcac
     9961 ccattgatac ccatacatca gaataaagga cgtctgctcc gtctacacct ttatcaacat
    10021 cgtcggtaat catcaattgt gcaccggatt tttcagcaaa gccttctgcc attttgacga
    10081 tttcttcttc aggttgtaat tcttttggtg caacgatacg catattcgct cctaaaattg
    10141 cacctgtgac taataaagag ttagccatat tgtttcgacc atctccgcag taagcaacag
    10201 tgataccttc tactgttcca aagttttctt ggatagttaa aaagtcagcg atcatttgtg
    10261 ttggatgcca ttcatcagtc aagccattcc aaactggaac accagagaat tctgctagtt
    10321 cctctaccat tttttgactg aatccacgga attcgattcc gtcaaacata cgtcctagta
    10381 ctttagctgt atcttctgtg gattcttttt tacctaattg gatatcattt gcacctagat
    10441 attcaggatg tgcacctaga tcgatcgctg cagtagtaaa tgcagaacga gtacgtgtag
    10501 atgttttttc aaataaaagc gcaatatttt ttccttctag atagtgatgt gggataccac
    10561 gttttttcaa gtctttcaag tgttctgaaa aatcgatcaa atattgtaat tcttcttttg
    10621 taaagtcttt ttctgctaat aagcttcttc cttggaatac tgattctttc atattaaaca
    10681 ttctccttta gattgttttt atttaagatt aaagaggaaa tcccgacggc agctgacagt
    10741 aagactgccg tcaggaaaca aattacttca agtcttcacg gacaagtggt tggctcatgc
    10801 agcgtggacc accacgacct cgtgacaatt cacttgaatt gatttctaac actttgatgc
    10861 catagctacg aagcaattcg ttcgatacat aatttcggtt ataagtgact acaacacctg
    10921 gagcgattgc caatgtgttt gatccatcat tccattgttc tcgaggtgca acgatctcat
    10981 ctccattacc agttgggatc agtacaagat catctaatcc aagagcagct ttcatcgttt
    11041 cgtgtaagtt atctgaatga gtaatcttga tatcatcacc atctggttcg attgtaaaga
    11101 catccatctt accgtcttta ctttgaattg ctggatggat agtgaattta tcgtgatcta
    11161 ccattgtaaa tacggtatcc aaatgcatca ttgcacggtt gttcggaatt ttgattgcca
    11221 acactttttc aaatcctgaa ttttttgcga ataggtggcg tgctaatttt tctaaagctt
    11281 tcgcattagt tcgttgagaa atcccaacag ctactacttt atcgcttaag atcaattcgt
    11341 ctccgccttc gatatgatct ggattttcac gatccaacca tacttcgatt cctttatttg
    11401 caaatcttgg atgatgtttc aaaatatatt ctgtaaacat cgattcacga cgacgtgctt
    11461 caaaagtcat cttattgact gtcatcccat ttcccattga tgcggaaggg tcgcgtgtaa
    11521 agtacaagtt tggcattggg tccatgtaaa atggatagtc atcgtctgct gacagatcat
    11581 atagactgct agaacgtact tcgatatctt ttgtacgaac cccagccatg attttatcta
    11641 ccatttcttg cgtttccatt gatagaagga actcatgaag accctctctt acagcattcg
    11701 atgcaatatg agattcatcc aacattttat tcaagaattg ttctttgaca ttgcctgcgt
    11761 caatcgcttc tgccgctaat ttttccaagt aaagagtctc gacgccttca ttttgcaatg
    11821 tcttcgcaaa gttgtcatgt tcttcttgag caatcggaag atatggaata tcatcgaaca
    11881 acagacgatc cataatatct ggtgtcaaat tttccacttc ttgtccaggc cgtttcagta
    11941 aaactgtctt taatttccct atttcagaga aaacgtgaat aggtttatcc atgttgtgtt
    12001 gcctccttct ttttgattac atgtatagga tatcctcctt ttgtaatcac tttcataatt
    12061 ttgtctcatt caaaatgaga cattcctcac attaatattc gataattaaa taattaaacg
    12121 tttttctatt tttaagcgta atttattc
//
LOCUS       DACDRK010000076        12117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDRK010000076 DACDRK010000000
VERSION     DACDRK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.076"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..379)
                     /locus_tag="IWJ77_002236"
                     /pseudo
     CDS             complement(1..379)
                     /locus_tag="IWJ77_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
     gene            complement(610..1740)
                     /locus_tag="IWJ77_002237"
     CDS             complement(610..1740)
                     /locus_tag="IWJ77_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9453814.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(2281..2487)
                     /locus_tag="IWJ77_002238"
     CDS             complement(2281..2487)
                     /locus_tag="IWJ77_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453815.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2503..3117)
                     /locus_tag="IWJ77_002239"
     CDS             complement(2503..3117)
                     /locus_tag="IWJ77_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9453816.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(3264..3947)
                     /locus_tag="IWJ77_002240"
     CDS             complement(3264..3947)
                     /locus_tag="IWJ77_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9453817.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(4056..4514)
                     /locus_tag="IWJ77_002241"
     CDS             complement(4056..4514)
                     /locus_tag="IWJ77_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9453818.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4598..6361)
                     /locus_tag="IWJ77_002242"
     CDS             complement(4598..6361)
                     /locus_tag="IWJ77_002242"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9453819.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6582..8042)
                     /locus_tag="IWJ77_002243"
     CDS             complement(6582..8042)
                     /locus_tag="IWJ77_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453820.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(8250..9176)
                     /gene="glsA"
                     /locus_tag="IWJ77_002244"
     CDS             complement(8250..9176)
                     /gene="glsA"
                     /locus_tag="IWJ77_002244"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9453821.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(9199..10626)
                     /locus_tag="IWJ77_002245"
     CDS             complement(9199..10626)
                     /locus_tag="IWJ77_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9453822.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10657..11871)
                     /locus_tag="IWJ77_002246"
     CDS             complement(10657..11871)
                     /locus_tag="IWJ77_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9453823.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3673 a         2488 c         2003 g         3953 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta ggaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 acttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgtttttttg ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag
      481 caccacgggt ataatatgat tttttgagat actttcattg cgaattcctc tttctaaaag
      541 aacctctcgc ttgaaaaata aaagattgta ctttatgata attcaaactg agagtttctc
      601 agtttctctt tattgttttt caggtcgata gagcgccaac tcttcggcct gtatctttga
      661 agaaatattc aacgtgattc cgattcctaa agaaaggatc agataacttg ttcctccgta
      721 gctaacaaat ggcaatggca caccagtcat tggcatcagc cccgcgatac ttcccacgtt
      781 cattatcgtc tgaacgaata gcaaagttcc gaatcctaaa agaaacaatc cagcttgctg
      841 atttttacag cgactgctta aacaaaagat tctcatgcat aatgagaaca ataagaaaag
      901 cacacacaat gcaccgatta gcccaagctc ttctgtaata atcgaaaaaa taaaatctgt
      961 ttctgtctct ggaagatacc ctttttttgt gatactattt cctaatcccc gcccaaatat
     1021 gccaccatta tatagtgcat aaaacgaatg ggtcatttga tagcccgcac catgactatc
     1081 gataaaagaa tctcttaaag tcgcgatcct ttcataagca tgattaaaca tctgtggaag
     1141 ccaaccttta tttcctaaat ataatacccc gcctgctgct ccaatcagca atgcagaaaa
     1201 agtaacgatt agatagatcc cttttttaaa tggaaccgcc gctgcccaaa atatgaccca
     1261 cgcaatcgaa aggatcatca atgctccagc aatctttggc tgaaataaaa tcagaaaagt
     1321 tatacctact gtaatcagga atggtttttt tagatctttt gggctactat ttccgtcacg
     1381 aaaaaaccag cttaaataaa aaatcaaaaa aagatttgcc agttcagaag gctggaattg
     1441 aatgccaaac agagagatcc aacgttgtgc gccgttgaca gtgacaccga atatccctat
     1501 tcttactaat actaaaaaga aaatcgataa ggctaaaccg tatcctgcta ttttagggtg
     1561 aagtaaatag tgtagtttga ctgaacgagc taagaagatc acgctccaac tcaaaaatat
     1621 gaaaattagt tgacgcaata atagactttt tgtattttca tcggcttgca ataaacgata
     1681 agaactggca ctgtatactt ctaaaagacc gatcatggac aaagtcaaat aaggacccaa
     1741 aatccaccaa tcgatctttt ttcaccgcat ctcctcctca cacagcaatc cacttgtcag
     1801 gtgaggagcg agtttgtctc atcaaataag tcagtcacaa aaatataaag aaaaggtcag
     1861 aaaccttttc gaacaagaat tctgacctag catcatctca tcgtccgagt tttagcacta
     1921 tacaacgtaa agaaaacatt cttagctatt ggaaaaagcc ttaatccaac agtttctgtt
     1981 ttacccattg gcgactttcg acgtttctgg gcaacagcct atgtttgtat aggagcctca
     2041 cctaacaagg ttattactat attctattta cgagttcatg atagactttt cctaatgatt
     2101 aggcaatgga atttgaaatg aaatagtgaa atttaacaat ctttaatatt ttttagaaaa
     2161 aaataacaaa taagttgaaa gaatcccgtt gcaaattctt tcagttgcac caggattctt
     2221 tgataatttt ttagttttat gtgttttttt atatgttttt tcacttttcg gaataaatat
     2281 ttatttgtta aagtaattaa acaatccgct aatcgtttga tctggcattt ctatttcctt
     2341 accatctaaa gtaacagtag taacgacaaa atggtcttgc aatggcggaa cttctaagta
     2401 ctgcccttgc agaaaatcat tcactgtttc atattctttt ttcatttctt ttgaatctgt
     2461 cgatggttta tagcttattt ttaacataac tagtgctcct ttttattcgg gacgtgcaat
     2521 cgtgaaaatt tctcctattg ctgcataatc atttcctgcc aaacggctta cagcattcaa
     2581 ttttcttgga tcgatccttc ctgtcttatc atcatagact gtttcattta aatgatattt
     2641 ctgtacttct agcaaaaata aatcagctgt tactttattt ttattccgta tctcgacatg
     2701 ttcatataag gatgtttcaa aacgaacttt tgcttctgtc aatgctggta ctgaaatgat
     2761 tactgaatca gttaaagtca agtcagtcag gtacagttcg ctttcctcat taggcagatt
     2821 ggctgctgtt ttatttactt cttctacgtt gtcctcatca aggatatgga taacagcttc
     2881 tcttgtctca agaatattct ttgctgtgtc ttttactgca tcttgttttc tctggatcgc
     2941 caaggataag atgggtcgat tcgaactgac gatattgaag taactgaagg gtgcgatatt
     3001 cactatatct tttgctgatt tagtcgttac tactgctact ggtctaggga tgactgaacc
     3061 aatcagcaac ttgtaatttt cacgttctgt caattctgaa ggactccgtt caaacatggt
     3121 ttcgccttct ttctataaaa ttcgttaaaa agcagatctt gaagacaaaa tgaaaagtaa
     3181 tgcttttttc atcttcttag aatctgcttt aaactatcta tcaatttttc ttttttgtca
     3241 agaatctgtg ccgtgcgtgt tttttagtta tttgctttac ttgcggcttc gacatatctt
     3301 ttagtgatca attgcggacg ggtgataata gatccaacta cgactgaaaa agcacctaat
     3361 tcatatgctt tctctacttg ttcaggagag tcagtatgtc cttccataat tactgggata
     3421 tcgacttgtt ctatgatttg attcaataaa tcaaaatcag gtgtctgcgt ctttcgtgaa
     3481 tattctgtat agcctgctaa agtcgtagac aaaagatcaa aaccatcttc ttgcgcttga
     3541 agcgcttcct caaaagtcga aatatctgcc atagctaacc gaccgtgttc gtgaatcatt
     3601 tggacaagtt cagcagttgt tttttctgtc ggacgtttac gagaagttgc atccagtgca
     3661 atgatttctg cttgcgtctc caaaagttct tccacttctt tttctgtcgc agtaataaac
     3721 acttctgacc catcgtaatc tcttttgata atccctatta ccggtaaatc aacgattcgc
     3781 ttgatttcat tgatatcttc tgtcgtattt gctcgaatac caactgctcc gccttctttt
     3841 gcagccaaag ccattctccc cataataaaa gaactgtgta gcggctcgtt ttccaaagct
     3901 tgacaagaaa cgacgactcc gcctttgatt tgttccagtg tatccacaat atctcctcca
     3961 gtaatgttat ataaaaatac agcttcatat aaaaatgtaa cccttttcta ttcaggagac
     4021 aataatgagc tatttctttc agcagacctt tcttactaaa agaaaattac ttcaaattct
     4081 tcgtatacga ccagcttctc ttctgtaaat tctatgttca acgcttcttt ataagcatgt
     4141 ttaaaaaact cagcatggac tttccgccaa taagctaatg tccgatcccc ttctccttct
     4201 ttccaagcat gctctttcgt gacttcacta aaaggcaaga tggtcacatt tatatttttg
     4261 atgatacaaa ctggttcatt ttttccattc aggaccacgt ctaattgacc aacttttggt
     4321 aaaggctcat tctcttgttt atacagatcg taaccacttg atgtaccagt ctttatcttt
     4381 tttacaacta gttcgctcaa ctcgtctgcc attttttctg tatccccaaa tgaaaagaaa
     4441 cctaattttt ctgattgata actaggatat ttttcttgga actttttgac atattcttct
     4501 acttgctgat acataacatc cacccctttg ctttttataa tgaaaaaacc atccagatac
     4561 tagtataaac aattatctgg atggtgagca taatgaatta ttgaatattc aagacacgca
     4621 gactatcttc aacgatttgt tttggtgtta aatgataacg ttcataaagc tgccctacag
     4681 gaacgctatc cgtgaattct ttctctgcac caaaatttaa tacgttcatt gaagaatcac
     4741 cataataacg actgatattt tcgccaaatc ctcctgacaa actaccatct tctaatgtga
     4801 tgactaattc gtgtgtttct tttaatttat ctaatgtttc tacgtcattt tctgtgatcg
     4861 ttcctggatt gatcaacgtt gcttcgattc ctttttttct aaaagctttt actgtttctt
     4921 ttcctaattc aagaaaaccg cctaaagcta aaattgctac ttttcctcca ttatttactg
     4981 tttgataggc tggaatacta taatcatttg cgatagctgt accatgttct acaccatgtg
     5041 taggaattcg gattgctaca ggctgcgctg tttgttcgaa cgcccaatct agcatagaga
     5101 tcatatcctc ttttgttgct ggagcaagat atttgatgtt cgggatactt gataagtaag
     5161 gaatgtcaaa tgttccttgg tgtgttcggt cactagctga gatcgttccg ccaccgacta
     5221 aaataatcac tggattctcg ttgatcgcta aatcatgtga taattgatca tatgcccgtt
     5281 gcaagaaagt acttgattgg aagacgactg gacgtactcc ctgagaagcc aaagcagaag
     5341 cgaaagtgat agagtgctgt tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt
     5401 cttggaatgt tttcagtcca aaggcgcctg gtatcgctgc attgactgcg gcaataggag
     5461 ctgtacttgt ttgcattttc ttatccaaat aatccatgat gatcatatta aatgtttctc
     5521 cagaagttac ttgctttgcc tccccggtgg acagatcaaa tggtacatgc caatgatatt
     5581 tcattttatt atcgattgcc ggctgataac cgcgaccttt ttccgtatgg acgtgaagta
     5641 caatcggatg atcaatatct tttacttctt caaataaacg gatcaccgtt tccagatcgt
     5701 ttccattatc cacgtaacga taatcaaatc ccattgcttt aaataggtta ttgctgcttt
     5761 taccgttggt ttgacgaagc tcagctaagt ttttatataa actgccatga ttctcatcaa
     5821 tagacatctc attatcgttt actaatacga ttagattgga ttgtaaaaca ccaccattgt
     5881 tcaatccttc aaaagctaag ccaccactta gggagccgtc accgataaat gctaccacat
     5941 ttcctgattc tttcttcaga tctctcgctt tagctaaacc tgttgctaag ctgatagatg
     6001 tagatgtgtg ccccactgta aaatagtcat gtttactttc atgttggttg gaatatccgc
     6061 tcacttcacg gtaatgtcct ttttcaaaac cattttttcg accagttaaa agcttatgac
     6121 cataagactg atgggaaaca tcccaaacga ttttatcctt tggtgaatca aatacataat
     6181 gaaacgcaat cgttaattct actacaccta gattcggacc aacgtgccca cctacatcgc
     6241 tgtccttttg caagattttt tggcgcatct cttctgccaa ctgtttcatt tcctcaacag
     6301 ataatttttt gacatctgcg gggctattga ttgcatccaa tacatgtaat actgtactca
     6361 taatacattc tctcctccgt actatcttct tttaatgaaa atagatttgt tacactttat
     6421 ctattctact acttagagtt gactctaagt caatagcatt ttaaaaaaat ttcagagaaa
     6481 gcttgttata taagtagatc ttcgcacgaa tcaggactca aataaaaacc ttctacagac
     6541 aattaaaaag ctttgtagaa ggtctcctaa ttaaggtctc atcaagaaat atgttttttt
     6601 aataggtgaa gttcctgagc aatatgatac aaatggatgt ttgcaccttt taatgagatt
     6661 ggatagatct ttgtcttttc agtttctaat ccattttgat agctagcagc atcgcacaaa
     6721 accactcttt ttttcgcaca tagacattca tcaggcaatt cttctaatga acgaaataaa
     6781 ataggattta tttcagcgat gccttcttcc gcatacaaat gaaattgggt tcgataaaaa
     6841 aattgtttga ctgaactttc tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt
     6901 tcactgttga tcaaagaaag acttttgacg aattttcgaa tgaaattttc attccatctt
     6961 agttggattt gatttttctt tttccactct tccaatattt ctacaatttt tccgtacaga
     7021 agttgttctt tttctgatag aagatagatc ctttccggta aaaatacttg tccatctgcc
     7081 cacaacgtac ggacaaattg taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg
     7141 atttcagcga tactaataat ctgtaaaatc aattcattca gtgataggtc attgagaaca
     7201 aaatttttat aaacaacttc gaaatctttt cttaacagct ccatgttctc attggtataa
     7261 tttctactgt tgaacaaaga aaaaatataa tagacatctt cttcttgtaa gtctagaatt
     7321 cctttttgct cttccaatcc ttgacagact aattgaaata acggcttcgt ttctgcgctt
     7381 ttcttatctt cttctttcag tccaagggga aatgcctttc ttccgattgc gatctctatt
     7441 cctcgtatga taaaggactt ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct
     7501 acataatcaa tcaactcgtt acaagcttct tctacatacg gatttttatt ttcatagcct
     7561 tcccaattga cataacgtac taaagctagc aagatgttcc gcgagtcaaa ttctgggata
     7621 atgatttctt tatttttcaa ataatcattt tctttaaaaa aatccaatac gatttttttg
     7681 attgtatata ctttgctcaa agaaataaat tcaagatcag ccagttctgt actcgtgaat
     7741 ataccattaa aataataact taaaaacttt aaaaaataag attgaccgta cagaaactgt
     7801 gtgagctcag aaagagcaat accatgtttg aatacgagtt tatacttatg cttctctctt
     7861 acttgttctt ttatacaata ttctagacac tcggctagac tttctagatc gtgaaggatc
     7921 gttggtgtag tcacccttag agaaatagct gtttgttcga tgtctatttc ttggaattca
     7981 aataattgtt cacacagata cacttttcta aatatatttc gctcgatgta attttccagc
     8041 attcctgata cacgcctcca gtttttctta tgattttatt taatcatatc tgtaaatcgt
     8101 atttttgttt cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg
     8161 gacaaaagtc aaacaagctc acaatttcag cctcattgta ggtagctttg agccgtttgg
     8221 acttttaccc cagcctcttt caagaatggt tattcaaaga tgtcagcgtc tagttctttg
     8281 actacatctt tcaacaattg gataccagct gcgctgttac caaatggatc tagtgccggg
     8341 ctgaagacac ctatgccgta acgatttggc acagctgcca tcaacccgcc gcctacaccg
     8401 ctttttgcag gtacaccaac atggacggaa aactctcctg attcatcata caaaccagca
     8461 gttgtcataa ttgatttgac gattgtagca ctttcttcag taatgatttg tttgccattc
     8521 caaggagcaa ttcctttatt tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg
     8581 acattgaggg agcattgtct gaaataagca tcgagtatct ccggtacgtc tccttcgatc
     8641 atttgattac ctttcatata gtaagcaaga gagcgattga tatcacctgt tcttgattca
     8701 gaatgataga tttcttcatt caatgtgata ttaggatcat cacagatctc tttcataaat
     8761 gacaagattc gctcaaattt ttcatctgca tctttaccgg caatcaacga ggttgttgca
     8821 atcgctcctg cattaacaaa aggattcatt ggatgtttgc gatgattgat ttccatattc
     8881 atgatagaat tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa gactttttct
     8941 attccgttgt ctttgattgc taataacaat acgggaactt ttgacatgct ttcgatggca
     9001 aagcgcacat cggcgtctcc tccctcaatc agttgttttg tatccaaatc ataaatcgcc
     9061 atacctaatt gtttgggatc cacttcagct aatgctggaa tgtaatccgc cacttgtcct
     9121 gtttgataat agtggaaatt ttttccaatc aattgttcta attcttttac ttccatttga
     9181 ataaccagct ttcatttttt atttttctgc atgctggaat ttatgacggc atgcgtagat
     9241 cgtaaatgga ataactaaga cgataagata agaaacaatc agaatcgttt cataaatccc
     9301 atgctgtcca gctgcgatac tagcaggtgg aacaaatgag atacaaaaag caaaaagtga
     9361 agtcaaaaga cctaaacaag caacgattac ttttacaact tttccgcctg gaacctgata
     9421 ggctctttca agactgtcat ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa
     9481 gtacaacatc aggtagatta caacagtcaa tgacatcgct gcaagaaatg aaagattatt
     9541 ccccccgccg cttaatgtaa agacggctgc ccagattgtt gcaacgatcc catttgcgat
     9601 catcaaatga gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc
     9661 ttcttctgct gctacgtgaa gtgcttttga aggagaggtt acccaagaag caatttctcc
     9721 gatgacacct aaacaaacaa gaatcgcaag aattttcgca atccatgcaa gatttggatc
     9781 aatgtgtctt aataggaatt ctaacgattg aatcacccct gtattaagag acaaaccatg
     9841 ttgaggtact gtagtagcga cggtcaatcc gccaaaagta tctaaaatga ttgccagaat
     9901 aaccaataca aacattgcta atggataatc ctttttaggg ttttccattt catttgcatg
     9961 actggcagat gtttctacgc ccatatatgc taaaataaag gaaacaaaaa caaccagcgt
    10021 aggcaatttc gaaaaatcag gaatcaatgt tttcatagac agtatcgttt gtagtggagc
    10081 gcctgaagcg acataatgga tccctaagcc aaacaagact aatgctggca acaagatacc
    10141 agctacaaaa ccgatccgag caatcaatga agtgtatttg gttcctttta actgtgagag
    10201 taaaatagcc cagaaaataa tcaataggat tgccaatttc agatatttat tgttttgtat
    10261 agcaccaata cccgttgcat aggataatgc accagttaag aaatacagca tggtcaagta
    10321 gccaactgtt atctcaaacc attggaaaaa gattgcggca aaaccgaatc gttttcccaa
    10381 tgtgcgactg acccaagtat agacaccgcc tttttcccat cctttcaccg ttgccatctc
    10441 tgctgcgcac aatgccacag ggataaacca tagcaaaccg cctaataaca agaaaaatac
    10501 taagctgaag cctgaggtcg cgaatgtggg atattcatat aatgaaacga ccattgagat
    10561 ggtcatagcc aaaaagccaa agaacgttaa tttttttgca ggaattttcg ttttgctttg
    10621 ttgcatgaat cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa
    10681 gaaaaagtcc aatccttctt cttgttcttc ttttgtagta gtgatcgttg taaagtgcat
    10741 cgccgatttt aaaatcactg ttgtcagcac ataggcatac acggcagtaa tgatgacacc
    10801 aatcacttga atccctaaga agtaccagct gcttggattc gttagtaaat ggctgttaga
    10861 tgcaaagatc cctatcagaa tcgtacctgt gaatccgccc atgccatgaa caccccacac
    10921 atcaagtgca tcatcccatt gtttcttttt acgaaaatca acagcagcat gacagacgat
    10981 acccgcaatg atcccaatca gcatggcact ttttgcttct acataaccgg cacaaggcgt
    11041 aatggctgcc aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa agccatttcc
    11101 ttttactttt gcaatgatca gccaagtaat catcgcaaat gcaagagcga tgaatgttga
    11161 accaaatgca ttgactgctt gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa
    11221 ccagccaaac caaagcagtc ctgtaccgat agcagctgcc ataagactgc tatgctgact
    11281 tctttctcct aaaggaatct ttctttgacc aagtgtgagg atacatgcta aagagccgaa
    11341 tccagctgtc gtgtggatga ctgctccacc agcgaaatct ctaaatccca tctgatccaa
    11401 aaatccgcca ccccatatcc agtggcaaac agggaaataa atcagcagat tccaaaaaat
    11461 aacaagtata atatagcctt ttaaattcaa tctccctgca aaagctcctg tcatcaaagg
    11521 aacggtaatc acgataaaca tcagttggta aagaaagaac attaaaaatg gaatcgttgc
    11581 accatgaaac atgttgggga agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt
    11641 tccaataaca ccgccgatat cccgtccaaa ggccaatccg aatccaccaa atacccacat
    11701 aatcgttgtt atcccaacgg agagaaaaga ttgagacatg atcgttagta tattattctt
    11761 ccgtgctaat ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa ccattgctgt
    11821 acataaaatc atgaatgctg tatctcctgt attgataggt aatgtattca tttttttcgc
    11881 ctcctttcta cgattacgag tataaaagaa ggtgtagttg tttttttctt ctgttttttt
    11941 gaattaatac ttgcatttat aaaagacaga caattaataa aagcttgatg tgttgtgctc
    12001 tttttcgttt ttcacacaaa taaagacaaa aaataaacgg tcttcttttt atagaaaacc
    12061 gtttattttc gtcttttttt atttttaaag atattttttc ataaggaata aagaata
//
LOCUS       DACDRK010000077        11922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDRK010000077 DACDRK010000000
VERSION     DACDRK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.077"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..274)
                     /locus_tag="IWJ77_002247"
     CDS             complement(<1..274)
                     /locus_tag="IWJ77_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9453824.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTY"
     gene            complement(390..1304)
                     /locus_tag="IWJ77_002248"
     CDS             complement(390..1304)
                     /locus_tag="IWJ77_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9453825.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(1395..1823)
                     /locus_tag="IWJ77_002249"
     CDS             complement(1395..1823)
                     /locus_tag="IWJ77_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9453826.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(1828..2202)
                     /locus_tag="IWJ77_002250"
     CDS             complement(1828..2202)
                     /locus_tag="IWJ77_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9453827.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            2488..2673
                     /locus_tag="IWJ77_002251"
     CDS             2488..2673
                     /locus_tag="IWJ77_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9453828.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            2685..2933
                     /locus_tag="IWJ77_002252"
     CDS             2685..2933
                     /locus_tag="IWJ77_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453829.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            2964..3143
                     /locus_tag="IWJ77_002253"
     CDS             2964..3143
                     /locus_tag="IWJ77_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453830.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3186..3656)
                     /locus_tag="IWJ77_002254"
     CDS             complement(3186..3656)
                     /locus_tag="IWJ77_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453831.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3743..4441
                     /locus_tag="IWJ77_002255"
     CDS             3743..4441
                     /locus_tag="IWJ77_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9453832.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4619..4960
                     /locus_tag="IWJ77_002256"
     CDS             4619..4960
                     /locus_tag="IWJ77_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453833.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4953..5624
                     /locus_tag="IWJ77_002257"
     CDS             4953..5624
                     /locus_tag="IWJ77_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9453834.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            5630..6316
                     /locus_tag="IWJ77_002258"
     CDS             5630..6316
                     /locus_tag="IWJ77_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453835.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLSH
                     ETIKKLRIGG"
     gene            6319..7149
                     /locus_tag="IWJ77_002259"
     CDS             6319..7149
                     /locus_tag="IWJ77_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9453836.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            7165..8016
                     /locus_tag="IWJ77_002260"
     CDS             7165..8016
                     /locus_tag="IWJ77_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9453837.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            8013..8372
                     /locus_tag="IWJ77_002261"
     CDS             8013..8372
                     /locus_tag="IWJ77_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP9453838.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            8387..8548
                     /locus_tag="IWJ77_002262"
                     /pseudo
     CDS             8387..8548
                     /locus_tag="IWJ77_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            8545..8850
                     /locus_tag="IWJ77_002263"
     CDS             8545..8850
                     /locus_tag="IWJ77_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453839.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            8850..9161
                     /locus_tag="IWJ77_002264"
     CDS             8850..9161
                     /locus_tag="IWJ77_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453840.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            9328..9744
                     /locus_tag="IWJ77_002265"
     CDS             9328..9744
                     /locus_tag="IWJ77_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9453841.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            9744..10412
                     /locus_tag="IWJ77_002266"
     CDS             9744..10412
                     /locus_tag="IWJ77_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9453842.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            10409..10786
                     /locus_tag="IWJ77_002267"
     CDS             10409..10786
                     /locus_tag="IWJ77_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453843.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            10783..11082
                     /locus_tag="IWJ77_002268"
     CDS             10783..11082
                     /locus_tag="IWJ77_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453844.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            11324..11740
                     /locus_tag="IWJ77_002269"
     CDS             11324..11740
                     /locus_tag="IWJ77_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9453845.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         4211 a         1837 c         2481 g         3393 t
ORIGIN      
        1 aatatgtaga ctccttaact tgtcttttgt aaataactaa ccattcttcg acaagctcat
       61 gaaatgttag atcaggcttc tgaagtgttt tttcgtttaa ttttatatca atttttttat
      121 ttaactctag caatgccact ttctgagttt ctcgagattt atttttataa gtaatgctca
      181 ctttttttct ttttctagtt tttggatcaa tatatcgctc attatattta tatacttttt
      241 ttccgttttt atcagttttg gtttcaatcc acattgtttt catctcctat ttgttgctat
      301 aataggaatg gataagtaag ccaattatag caggtttatt tttcatcacg tccacaaact
      361 ttggcgaggg agggggcgtg tttttttgtt tagtagctac ctttccatga agtatctgcg
      421 aagaacactt caaatgttgt tccatcattt ttcacttcaa aatatgctgt tccttgtgcg
      481 gatttacccg gttggatggt ttccgaaaag taatctctac tagctaaatc agatttaata
      541 tctgatgaat cataaaattc taaataactt gaacttgcat caaaaggtgt agtccctgtg
      601 ttttctacaa gaaaatctac ctttgcgtaa aataggcctt ctggtttata gaaatcatca
      661 ccgtaatctt ttgatacaga ctggacagtt acgttaatag agttaccatt ttcagaagta
      721 aaaggaactg cttctcctat actatgtgca actgatgccg tctggggttc tgaagacgaa
      781 gttgtagttg tagttgtagt agagcttgaa tctttggatt ttccatcttt atctaagaaa
      841 gttcgttgat tataatatac aacctgttca gtttttggac taacccaaat taacattgtt
      901 tcatcatcag aatcagttga ataagtatat tggagcatct ctaatttttt tatatctttt
      961 gcactatttc catgttcaat taattcagag tgctctttat aataatctcc ttgtgcatcc
     1021 tttgaaatag atacaattaa ttcatcccgt gtgatagcat tgtctaattc tttgtaaaca
     1081 aaatcatttg tccatttacg taacggtgaa ccaagtgtct gataaacttg agttaaatca
     1141 tcaccaactt ttattttatg aacgtctgat gaagttagtt tttttgtttc atattcagat
     1201 gttgatgaaa cagtattttc tttagtgcta gcagaattag gactagtgga gcatccgctt
     1261 aataccaagc ctaaaaaaag caacccaaaa acaatttttt tcatttttaa ttcctcactt
     1321 ctatgatatg atttttttgt aggatcttag aaataaggtt tttagtccgt gttgcagcac
     1381 ggactttttt tacattatat aagaaagcga taagcgcttt ctggaagtcc gtaaagattc
     1441 tttaattcct caattcgttt aggatattga tcattgtctt ctttgtatag agaaacaatg
     1501 agattagcag caaagcaatt agcttcgctt tcagatttgc ttctagatgt tcttgttgat
     1561 acatagtaac tggataagcc acgatgaaaa atagcgtgac ctaattcgtg agcgcaaatg
     1621 tagaatcttt cctcagagtc tctcagttca tcatttaaga agattattgc acgacctcta
     1681 atttcttgaa actgtccttt gggattctcg ataaaaggaa cgtattgaat tttaatgccc
     1741 atcttttcac aaatataaaa aggattagcg gactggtatt tccgcttcaa ctcctcgact
     1801 aaattaatcg tatccatctc cataagctca catctttttg cctttttctt tgtcttcttt
     1861 cacaatatcc cagaaggttc caataaggat atcttttacg cgttggacct gttcaggcgt
     1921 cagtgtttct ccgccataag acatattgac atttgaatct agtaatttat caagttcaac
     1981 tacttcatct tttgttgccc agtcaggaac ttggtttcta ccgagtaaat aatcagtagt
     2041 tacattaaaa taatctgcta caagttgtag tcttttagta ctaggagttc tatttttcca
     2101 ttgataaatc gtgtttttag gtatatttaa ttcttcttcc aattgagtta tacttatgcc
     2161 tcttttatga gctaattcct ttattctatc tagtaaattc attttcgcac ctcacaagct
     2221 acgaaaacac taataaaaaa attagttaaa ggtgttgaca actaataaaa gtgttagtat
     2281 actgttttcg taagctaaaa tattagctaa aaagacttga taactaataa acacttcacg
     2341 gtcggcaaac tctgaaatgt aaattactag gcatttctgt gtcttattta gctatgtcta
     2401 tatactaata aaaatattag ttattgtcaa cgaatattag ctaatttttt agcttacaga
     2461 ttattttttt agaaaggagc tatttttatg tctgagaatt tagacttaaa aattcgagcg
     2521 gagatgagaa aaagaagaat gactttcaaa gaactagctg cgcttatcgg tatttcagga
     2581 gcttatttat cagatattct aaacggcaat cgtgatggaa agaaagcaca acagcatatc
     2641 gaaacagtga aaaaaatatt ggacatccga taggggggta ggcgatgaca aaactaaaaa
     2701 aacaagattt tgtaaaaaaa tacaattatt ctccatctac ttatcaacgt cgaatgtcgg
     2761 aactaaaaaa tacagcaatt ttctcagcgg cgtatgaacg ggtcacagga caagaagttt
     2821 ggatcaatac agaattatac gataaatttt tgtctttcaa atcctataac aggttacgca
     2881 caagaaaggt aacacctaaa gaatttatcg agaagcattt agttgattta taaaaaacag
     2941 aacattttga gaggtgaagg ttaatgggta aattcaacag agcattggta ttcagcgcac
     3001 cgctaatcat ctacgcttta ggactttggg gaagcaggca agcgttgata ggaacgattg
     3061 tttacatggt ttggattttt atggggcttg atgaagctga agctgagtac agagcgaaaa
     3121 agccaaccga gggggctgac taaaaatgaa tagaaaagag aaactagaat gtatattact
     3181 attactcagt ttaattcttt cactaatttc tctattggga agtttgtatt tttgatatca
     3241 acaattttgt ggatttgggt tctatttaga gtaaatatca tttttgtttg gtgattaagg
     3301 agtttattaa aattaatttt ccctacttca aatgcaaggt aacaagatat agcactttta
     3361 ggaggaatat ttagcggtac agcttcagag tatattctat tatcatcgac tgatcctcct
     3421 ttagcaatta atttcttaaa tggagtagct gaccatctgc ggtttaagtt agcggatgaa
     3481 agttctaaat taactaacat agctggttct gttgaatagt tagaaatgat tacttttgta
     3541 taaatcatat ctgcagcgaa gtaagaagca ttaagttcaa cttctagttg agggcgcatt
     3601 ccttttattt ttatccctgt taaaaacgta ctcaatataa aacttattat tgacatccat
     3661 tgaaaaattg ttaagtgaaa aaaattcaaa ataaacacca ccagttttta actaaattat
     3721 accaaaaagg agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt
     3781 gactacgagc ttacaagtgg cagaaagctt cgagaaaaaa catcaacatg ttttaagaga
     3841 tattgatgca ctaaaaaaag atgtgtccaa ttttggacag atgtttgtag aaggtaatga
     3901 accagattca tatggcagaa atcgacgagt tttcttcatt agtagagatg gatttttctt
     3961 gctggctatg gggtttacag gaaagaaagc tatctacttc aaacaaaaat acattgaagc
     4021 attcaacgaa atggaagatg ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat
     4081 gatgatctat caattagaag aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga
     4141 tactatgcga attagcggac aacaagagtt tgaaattaag caaaaaggaa atatgaaagt
     4201 tatgggagtt ctagggggaa aagaaagccg agcttatgaa gaaatcagca aaaaagtatt
     4261 ctcaaaattt tggtctgaat ttaaacgtac cttttcaatc ccaagatatg gcgagttacc
     4321 tcgtaagaga ttcgatgatg ctgtttcatt tattgaaatg tggttgccag aaactgcgat
     4381 ccgtatggaa attgatcaac tgaacagaca acagagactt tttggtgatg acaatgaata
     4441 gagctgaagc gctaagaata gggacggcaa ttgctaattg ctggtggaaa tactataaac
     4501 caatcatcct aagccaacaa catattgaca agcaaaaagc atggcaacaa ataaaaaagt
     4561 gactccgccg gcaagcaaag agtcacaaag aaaacacatc ataaggagat tttagcatat
     4621 ggaaaaagaa ctttccactc tagatcaata tttgattgat cctgattggg gcaagccgaa
     4681 aattgaggaa acaagtggtc gaaaaatcag acgaaatctt ttgacgaatg aagaactagc
     4741 ttgggatcaa gatgatttag gcaaccatgt aactatttgg gatcatgttt atcttatcca
     4801 tctatcgaag cattcgaata aacctgaata tatttacgtc atcgaagatg gcttgattga
     4861 tgcgctagaa gagtacgaca gagataactt gattgatatc tcttattacg gaccaggtaa
     4921 gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aatcaaaggg acaacgaact
     4981 ttgaaaaact ttttagtcgt aagttaaata aaattctcaa gaaaaaagga aattttgatt
     5041 atttatcttg ggctcacgcg tgggagatta tgaaaaagaa tgatccacag gcaacggtaa
     5101 ctattaatga gtacaaacac tacagggttg tttctggaac tcatcaagac tttcttgttg
     5161 aggaatataa accttttctt atggatgaaa ctgggactta tgtatctgtc tcagtaacgg
     5221 ttaaaggaca cacggaaacc gaattatttc ctgttttaga ttatcgaaac caaccagttg
     5281 ttaaaccaaa tgcaatgcaa atcaataact cattgaagcg atgctttgtg aaagcattgg
     5341 ctctacacgg actgggatta tatgtatttc aaggggaaga tattccaaca ccacctagaa
     5401 tcgatacaaa gaaattaaac atgctagaga cgattctaga agctttcaat gagcagatgg
     5461 gtaaagatat gaccaaaacc ttaatcgaat atgttaatga gcagacagat aaattagggc
     5521 tcttagctga taacgttgaa actattgaac agttaagcta tgagcaatgt gccttgatgg
     5581 agcgagcaat agcagctaag agaaaagaat tagataagaa gtgatatgag tgtttaaacc
     5641 attaatcgat tcatattcag cggttctgaa aaagttcaaa ggaaaagaca taagcgcaac
     5701 catcaatgag gaagtgaaca ttgatcgact aaagacgatg tatgacggct acgatggtga
     5761 tcgagtcatt gaaattcgtt ttattgatcc tagacgtttc accgtacagc aacgaaactt
     5821 catctatgcg ctgataggcg atatttttat cgatacaggc atgccaacgg acttctggaa
     5881 ggaattcttc tacttccgtt ttgaaggtgt cacagggcgc aaaataagcc tcaaagatga
     5941 atcgaatacg actgtgagtg atgctaatgt cttagcaaat atcatcttag atttcatctt
     6001 tgaacatcat attcctttca aagaaggcta tgagatttta ccagcgaatc aagaatatta
     6061 cttctacaaa tgcattacaa aaagagtttg ttgcatctgt ggcaaaacag gagctgacat
     6121 cgatcacttt gacaaagcgc taggaagacg aaagcgaaaa gaagttgatc attcagagta
     6181 cacatttgca gcactctgca gaatccatca cacggagaag cacaaaatag gtgtgatcaa
     6241 ttttaaaaat aaatatcaaa tcaaaggaat caagttaagt catgaaacga ttaaaaagtt
     6301 aaggatagga ggataaattt ggctgagata agttggatca aacttaaaac tactatgttt
     6361 gacgatgaaa aaatacggtt aatccaagct gttcctgagt cggatgccat catcgttata
     6421 tggattcgat tactagtttt agcaggaaag actaacgacg atggtctgat atatatccag
     6481 aggaacatgc cttataccga agaaatgctt gctacattgt ttggcaaaaa cgtaaatacg
     6541 gttcgcttag cgctaactac attggcaaat ttcaacatga ttgatctaag cagtgatgga
     6601 ctaattgcca tcagtaattg ggaaaaacat caaaatatcg agggtatgga taaagtaagg
     6661 ctaaaaaatg ctgaaagaaa ccgtaaatac agggaaagaa agagacagga acgtctcaaa
     6721 ttggaaaatg acgttagcgt gacgtcacgt gacggtacag ataaagatat agaagaagat
     6781 aaagatatag ataaagaaga aaagaaaggt aagtattctg acgaacactt acgccttgct
     6841 aaaaaaatgc aaaggaattt aactgaagat tttccaaaag aaatgaacaa agtagatatc
     6901 gaaaaatggg cagacacaat caggttgatg gaagaaagag ataaagcatc tatagaagcg
     6961 attgagtatg tgatcaattg gctacctaca aatgaatttt ggtttggaaa tattagaagt
     7021 gctaagaaat tgagagaaaa atttgagaag ctcaaattcg aaatcaaagc agacaagaag
     7081 aatcataaaa agcaaagtca aaaactacag tacagcaatc ctagtgaata tgacgacttg
     7141 ccaatttaaa aggagatgca tcacatggaa agcctagcaa atgctatgga gaagctaata
     7201 agaagagtat tagtgcaaag cggaaaatgt ccagaatgta gcgaaccttt gtatagttgg
     7261 cgagctaaaa ataaggatgg ttcagaacgt tgtaaaccaa catgtatgag ttgtggttat
     7321 aaagcgttac gtgtgaaaga ggatatacag accgaacgga tatataacga cagcttaaaa
     7381 gcacgagcat tgagtttttt tcaaaatggt tcggtattaa cagataaaac tttgtttaaa
     7441 tgcaaaatgg agaattatca cgtagtggac caagaaacga aaattgcttt agaaagagct
     7501 aaaagctatg taaatgatgt cctactgaac catcctgcac atttcattct atcagggaaa
     7561 tcaggaagcg gaaaaagcca cttatcaatg gcgacagctt gggaaatact tgagcgctca
     7621 aattatgaca agaaaatact ttttataagc tatcaagagt tattagagca aataaagttt
     7681 tcttataaca atgctgaact gagaaaagaa attgaaggat cgcttatagc cgatatcaaa
     7741 acaactgatt tggtggtttt tgacgatatt ggagctgaat taggtagcgg ggtatcaaat
     7801 agtaggcaat ttacaaacaa cacgttaaac acgctcttag aagccagaca gaacaaggca
     7861 acgatcatca caacaaactt atctggtcct gaactaagag aagcctacgg tgaaagaatt
     7921 gtttctagga tatttaagaa ttcagaaggt tatgcgctga aattccaaca aacagcagac
     7981 aagcgcataa aaccagtgaa aggtagtatc gcatgaataa ataccgtaat aaaaaaactg
     8041 ttcatcgagg tatcaagttt gattctatcg cagaagcaga gtattacgat ctagccttgt
     8101 ggcaagctga agcgaacggc tggaaagtaa aacttcagga aagatttgag ctgatgccga
     8161 aatttgaact agacggaaag aagtatcgca agatcgagta tattcccgac ttcacatttt
     8221 ataaaaacgg caaactagtc aaagtcgtag atgttaaagg gatgcagaca aaagatttca
     8281 aaatcaaagc gaaactcttc tgcagtcaat atcgagtacc actgatctta gcgaagaaat
     8341 atcggaatac gttcaaggaa gaacggtttt aacgaggtga tccatcatga caacagaaga
     8401 agtgattcaa atgcgtattc gaagccttca gcgtgagatt gacgatctgt aacggacaaa
     8461 ggcagtgatg gtcaatgaaa cggcgaggaa ggcaatcgat ttgcacatag aaaatttaag
     8521 aaggaaaatc catcgattgg aggaatgagc gtggataaga aagcagcaat gaaacgaatc
     8581 atcgaactga cacattctga gaattggcaa gaagacaaag aaatagttgc agaagtccaa
     8641 aagctcggta aatcaatgtg ggctgaaaag cctaaacgga aaacgccgag aaaaattgca
     8701 atctggcatg gtgatcgaat tctagtaaca ggtactgctg aacagttatc tgaaattact
     8761 ggattaagca aaaacattat ctgggataga gctaggagct tatggattga ttcaaaagga
     8821 cgacagttta ggtatgtgga ggagaaataa tgaacgaact aatcacaaaa gtagagcagt
     8881 gggctaaaga caagggactg gatcaagcgg atccaaaagc acaatttttg aaagtagctg
     8941 aggaattcgg agaaattgca tcggcaatgg caagaagtaa tgatgagcta tttaaagata
     9001 gcgtaggaga cgttatcgtc acgctgatta ttctttccat gcaaaaaggg gcaaacatac
     9061 aagagtgttt agaaatggcg tataacgaaa tcaaaggacg cacaggaaaa atggtagatg
     9121 gtgtattcgt gaagtcgagt gatttggagg acagcgaatg atgatgccaa aagaaatcta
     9181 ttcagatgca ttaagaggtg ctagaaatca attgaaaatg gcaaaaagag catacgaaat
     9241 tcggcccaca atcgaaaatg aacgcagagt aaaagcaatt cgccgtagat gttcaattta
     9301 cggcgagtta cagaaggagg acagcaaatg ataccgagat ttcgagcttg ggataaacga
     9361 aagaacgtaa tgagagatgt agccgtcttg cattttacta aaaacggcaa aacaaacttt
     9421 attgaatatt ggataaatcc taccgaattg aaatcatatc acgtgcgaaa catcgagctc
     9481 atgcaatcaa cagggctgaa agataagaat ggcattgaga ttttcgagtg ggatattgta
     9541 tcagtcagcg tgcgaaatgg attcgattat ttagataata aagtttgtgt tgtcaaaaat
     9601 tcaataggac attcgggatt agtgtgtgcc actgttgatg aagatttaga gtatcaaatt
     9661 tttaacacag agctgtttga agaatacacg tatgaagtca tcggaaatat ttgggagaat
     9721 agcgagttat tggaggaaca gtgatgaata aacaggaatt gattgatgaa ttagctaaat
     9781 atgtaaagcg ttatgagaac gttatggatg agcatggcca aggaaggtac ggcgcttatg
     9841 aagtatcttt aaagttggtg aaaagactaa atgaatcaaa aattacggac gaacaagctt
     9901 ggaataaggt agctgaggct tatcctgaat cggcacaaag cttgagaaac actttagata
     9961 atgctgtatt tggtaagact ggtgaaccgc agaagcacgt tatgccgaag tttgtggcgg
    10021 agtggataga atacgctaaa aagaaaggtg atagtctagc tatttcattc aagccgtgga
    10081 acctctatgg tgttgagtat agcaaggctg atagatggat tggagataat caagaaacgt
    10141 ttgctcgtgc ttggctagac ggctacgagg tcgagaaaga gccgttgtat tatgtcccgc
    10201 taccatatga agtttgggat gaagaggcag cggagcttaa aacagagtat ttgtatctac
    10261 attatgagat cacaagtgat gaaactagaa tatttcctac aaaagaacct agaaaaggtt
    10321 ttgtcgcaaa attagatgaa cttacgatta aatcagcaga tgaaaactac tggccgtttg
    10381 ctgtaccagt ggaagaggtg gtagaaggat gaaaaaattg caccaactca tatctgagaa
    10441 agaaagcgaa ctccaaaatt tagaagatag tttgggattg gggtttccaa ttgttgagca
    10501 agttaaaatg gttcaaatca gtcatctaca acttgaattg gaagatttaa gacaaataga
    10561 agatccgtat caactcaacg ataaccagca aatcgtgttg gagtggttga aactaactgc
    10621 gtcaacagga aagccaatgc aagttgtatt ttggatgatg aataatgcag catgggggca
    10681 tttagacgaa ttgagggatc ctttgatgga attaactgac aaacagcaat tcgaagtcct
    10741 aacagcattc gctcaatggg gattagaaca ggaggaaaaa gaatgaaact aaaagacgga
    10801 ttttacgcta gtagccacgg tatcggcggt ttaatgctag acatgccgac aaagaaccct
    10861 aaaacacgta agaagccaaa agtcaaagtc ggtgacatgg ttcgctgtga agcagaagga
    10921 ttcatctatc catttcgtgg atatgtagaa cacgtctata atcactcagc gatcattcgc
    10981 attgaaaaca cgatggaatg cgacaagtta ttagcgaaaa gcaaagataa tttagcagtg
    11041 gtgagattgg tggatatgga ggttataaac aatgaaattt aaaatctttg aagaggacac
    11101 tcgctataaa ttagaaaaaa aattaaatga atttgcaaag aataatgaaa ttcagcatat
    11161 atctttaacg gcttctaagt caggttatac aacttactat gcagctgttg tgagctacgt
    11221 aattcaataa aaggaactcg gcaaataaaa aagccggatc actccgactg atgtaataaa
    11281 tccgacaagt ttattatatc acataaagga gcggtttgac ttgatgcaat tgttacgaga
    11341 ggtagatttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact ttcggcgctt
    11401 ggagcggatg gcaggtcgct ctttgataga tattaagtcg ccgattatta cggatatgcc
    11461 gaaggcaccg aagcacggca ataaggcaga agacgcgatc attcagatga tggatataga
    11521 agcggagaga gacgcgattt tagcggcttt gatggcgctt agtctgatta gccgtcagat
    11581 actctactac agcttctgtg acgtaaacaa gcactctaat tatgaaatag ggcaattgat
    11641 acgaggatac ggagagaaga atgtagagaa gctgaaatcc atcgcattga tcgaatttgc
    11701 tgaagcatac aaaaaaggcg tgttagttca gtatcgttaa ttttgtaggg tttttgtagg
    11761 gatagtgtag ggtttttgag cggtttaacg tgatattatg atagtgtcga aagattagtg
    11821 ataggtctaa gacaaaataa taataaaagg aacatcgttt tattattgtt tcacaattaa
    11881 gcttcgatag acagcagcgg aaatattaag aataaggatg tg
//
LOCUS       DACDRK010000078        11564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDRK010000078 DACDRK010000000
VERSION     DACDRK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.078"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(32..1081)
                     /locus_tag="IWJ77_002270"
     CDS             complement(32..1081)
                     /locus_tag="IWJ77_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9453846.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLRDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1163..1852)
                     /locus_tag="IWJ77_002271"
     CDS             complement(1163..1852)
                     /locus_tag="IWJ77_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9453847.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1958..2326)
                     /gene="rplL"
                     /locus_tag="IWJ77_002272"
     CDS             complement(1958..2326)
                     /gene="rplL"
                     /locus_tag="IWJ77_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9453848.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2379..2882)
                     /locus_tag="IWJ77_002273"
     CDS             complement(2379..2882)
                     /locus_tag="IWJ77_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9453849.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3292..3981)
                     /gene="rplA"
                     /locus_tag="IWJ77_002274"
     CDS             complement(3292..3981)
                     /gene="rplA"
                     /locus_tag="IWJ77_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9453850.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4085..4507)
                     /gene="rplK"
                     /locus_tag="IWJ77_002275"
     CDS             complement(4085..4507)
                     /gene="rplK"
                     /locus_tag="IWJ77_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9453851.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4850..5518
                     /gene="sdaAB"
                     /locus_tag="IWJ77_002276"
     CDS             4850..5518
                     /gene="sdaAB"
                     /locus_tag="IWJ77_002276"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9453852.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5531..6403
                     /gene="sdaAA"
                     /locus_tag="IWJ77_002277"
     CDS             5531..6403
                     /gene="sdaAA"
                     /locus_tag="IWJ77_002277"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9453853.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6494..7513)
                     /locus_tag="IWJ77_002278"
     CDS             complement(6494..7513)
                     /locus_tag="IWJ77_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9453854.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7686..8573)
                     /locus_tag="IWJ77_002279"
     CDS             complement(7686..8573)
                     /locus_tag="IWJ77_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9453855.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8650..9195)
                     /gene="nusG"
                     /locus_tag="IWJ77_002280"
     CDS             complement(8650..9195)
                     /gene="nusG"
                     /locus_tag="IWJ77_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9453856.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9296..9466)
                     /gene="secE"
                     /locus_tag="IWJ77_002281"
     CDS             complement(9296..9466)
                     /gene="secE"
                     /locus_tag="IWJ77_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9453857.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9490..9642)
                     /gene="rpmG"
                     /locus_tag="IWJ77_002282"
     CDS             complement(9490..9642)
                     /gene="rpmG"
                     /locus_tag="IWJ77_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9453858.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9804..10448
                     /locus_tag="IWJ77_002283"
     CDS             9804..10448
                     /locus_tag="IWJ77_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9453859.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10504..11007)
                     /locus_tag="IWJ77_002284"
     CDS             complement(10504..11007)
                     /locus_tag="IWJ77_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9453860.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11020..11066)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT         3382 a         2394 c         2108 g         3680 t
ORIGIN      
        1 acatgtttag acattttcct ctaggaaaaa attaactagc accacgggta aatacagtat
       61 tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg gcgacttgac
      121 caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc aaatggcttc
      181 cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata tacacgattt
      241 ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact gctccagaca
      301 aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg ggactgctga
      361 tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa aatccactga
      421 ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt ttaagtgaat
      481 caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc cagtaaagca
      541 gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc caagaaaaaa
      601 gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt aaaccgctaa
      661 gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca ccgtaaggaa
      721 tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat gcaaataacg
      781 taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct accattcgaa
      841 tgatcaattt tggacgatcc accacgtccc ttaaaggaac taaaaacagt aaccctaacg
      901 catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta acagataaac
      961 tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca acaacgccac
     1021 aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca ttactcttca
     1081 ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg aaatagaaaa
     1141 atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt ttcttgcgat
     1201 ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc aacgtgactg
     1261 cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac acagacgaag
     1321 ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc atcgcatctg
     1381 cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac attacaccta
     1441 tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg gttttcttat
     1501 ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga gcattaccca
     1561 aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg actccaacta
     1621 ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc aagagtatcg
     1681 atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc gctctcggat
     1741 ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc actaccagta
     1801 tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc atatttcctc
     1861 ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa agcaccgaaa
     1921 tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt acagatgcgc
     1981 caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg ccttctttaa
     2041 ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa ccagttgctt
     2101 cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat tctacagtga
     2161 attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct acaggagcag
     2221 cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt tctaggatag
     2281 ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata atttcctccg
     2341 tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc tccatcttct
     2401 ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg tagtacagat
     2461 agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt gatttcttcc
     2521 aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt tgcttcttta
     2581 gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa tgcaacagct
     2641 gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc tgcacgtgaa
     2701 agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg tttacgtaaa
     2761 cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga tgcagctgct
     2821 gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat tgcttcactc
     2881 atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga aaaggcaagt
     2941 cacttttata gttactttgc tcaaccagta ttattcatca cctattcact tcgttcatag
     3001 tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc agtattgttc
     3061 atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc accgcagaca
     3121 tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag gcttgatgcc
     3181 acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat gctaaactag
     3241 ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta attagaaaga
     3301 agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat ttttcatgta
     3361 ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg ctgcaaagtt
     3421 ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga tatttccagc
     3481 tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt ttgttacgtc
     3541 cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta atactcgtcc
     3601 tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa agtcaaacca
     3661 tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac cagctgcttc
     3721 agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt taccagttcc
     3781 gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt ctacgttcaa
     3841 acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt ttgctaaagc
     3901 tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg catcttgcat
     3961 ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac ggttgtacct
     4021 cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc ttagggtgta
     4081 tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg cgcattgctg
     4141 cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct cttacttgat
     4201 cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt tcgattttag
     4261 ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg aatgaacggt
     4321 cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct gtacgagcgt
     4381 tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca ggacctactg
     4441 gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt tctactttct
     4501 ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg catatttctc
     4561 ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa caatgcacac
     4621 tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat ttttacattt
     4681 tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc tcatttcttc
     4741 ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat aacacggttt
     4801 tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa tgaaagattt
     4861 aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa gtagctctca
     4921 cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg aacagcctga
     4981 ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc acggaacaga
     5041 tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt tagctgattc
     5101 tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga gtgaaaaagc
     5161 cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc tttcagtcac
     5221 tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat tcaaaatttc
     5281 tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag gtatgattgc
     5341 taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga cagtgacacg
     5401 ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc aagtagaaga
     5461 aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt tcgattaagg
     5521 agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag cttatccaag
     5581 tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc gtagtcacat
     5641 catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg agggaaaaga
     5701 aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag atgattattt
     5761 aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca atgctgtagc
     5821 tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg ccggaagcgc
     5881 gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga cacatgaaca
     5941 gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa acaacgcttc
     6001 tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg caatggcagc
     6061 agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag cagtagccat
     6121 tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag ttgaagtccc
     6181 gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg atatggcatt
     6241 agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt accaagtcgg
     6301 acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg ttacgccaac
     6361 tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct ccttaatttc
     6421 gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta attgcccttc
     6481 tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc gtttttgttc
     6541 ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca gatgttactc
     6601 cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca actaagtcag
     6661 caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag tcgaaaatca
     6721 ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag gacgcaggac
     6781 ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc cctgcagaga
     6841 tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga agatgggtga
     6901 tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct gcataaattt
     6961 ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac gcagctaagt
     7021 taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg taaccaccga
     7081 gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct ttgttcaaaa
     7141 tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc ggtgcgcctg
     7201 gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg gaaaagattg
     7261 atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt gccaatcctt
     7321 gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa acagatgtat
     7381 gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt gtattaaaag
     7441 aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata tagccaaaag
     7501 ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt acaatatcga
     7561 ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc atcttcttga
     7621 ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct cagatacttt
     7681 tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg gttttcatga
     7741 agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc atttccgcgt
     7801 tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc atcactcaag
     7861 tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg aatattaccg
     7921 atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt tgctaactca
     7981 tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc aattgctgtt
     8041 aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata gcgtagtgca
     8101 ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta gttttctttc
     8161 agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt tttattatct
     8221 tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc tgttgcttgt
     8281 atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg atttttttcc
     8341 aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg gaaaaaaagt
     8401 gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc tttatcagaa
     8461 acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa actgataagg
     8521 actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt catacacctc
     8581 ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc gacatttcaa
     8641 gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac tggtttcacg
     8701 tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt ctgttacttg
     8761 tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa cttctacttc
     8821 tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga cttcttctgg
     8881 aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg tcacaccagg
     8941 tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa gtacgtatcc
     9001 tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag tttctttttc
     9061 ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg cacgagattc
     9121 gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat accagttttt
     9181 ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac ctcagtacac
     9241 ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta gcgagttatt
     9301 ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac atcaatccga
     9361 aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt ctgcttggcc
     9421 aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga caacctccct
     9481 tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt tacaaaactt
     9541 attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt agttgcgaga
     9601 cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct gcccccttgc
     9661 tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt caatttgtga
     9721 aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa ttaagctaaa
     9781 atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag aaaaaagatc
     9841 tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta gaaacttccg
     9901 gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga aacatttaca
     9961 aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct gtcactcatc
    10021 ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa gtattcttta
    10081 cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt tatggtattt
    10141 tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa caaggaagct
    10201 atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt gcaaaaatca
    10261 tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa gacgaataca
    10321 ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt gaaacgatcg
    10381 tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg caaaaccaag
    10441 aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt tcgcttttta
    10501 taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca atggtgctga
    10561 aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc aaaatgcttg
    10621 gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa agaagatcgt
    10681 gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag ctgtcaggcc
    10741 taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa aaccaaagaa
    10801 ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt taaagacaac
    10861 taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata caggcgcaac
    10921 taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga ttacggataa
    10981 ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt ttttttaacg
    11041 cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca gacttttcat
    11101 ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat gtaaacgttt
    11161 gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa ttcccagtaa
    11221 agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata aataaaaaag
    11281 gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc tgaagatcta
    11341 agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat gcgcttgcta
    11401 ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt ctgctataat
    11461 aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt cattcaaaaa
    11521 atagaaaaag cttgctaatt attggttaac cagcaaacag gagt
//
LOCUS       DACDRK010000079        11358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDRK010000079 DACDRK010000000
VERSION     DACDRK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.079"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            612..1583
                     /locus_tag="IWJ77_002285"
     CDS             612..1583
                     /locus_tag="IWJ77_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9453861.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1661..1897)
                     /locus_tag="IWJ77_002286"
     CDS             complement(1661..1897)
                     /locus_tag="IWJ77_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9453862.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1866..1997)
                     /locus_tag="IWJ77_002287"
     CDS             complement(1866..1997)
                     /locus_tag="IWJ77_002287"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9453863.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2073..2456)
                     /locus_tag="IWJ77_002288"
     CDS             complement(2073..2456)
                     /locus_tag="IWJ77_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9453864.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2489..3274)
                     /locus_tag="IWJ77_002289"
     CDS             complement(2489..3274)
                     /locus_tag="IWJ77_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP9453865.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3452..4552
                     /locus_tag="IWJ77_002290"
     CDS             3452..4552
                     /locus_tag="IWJ77_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9453866.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4542..5366
                     /locus_tag="IWJ77_002291"
     CDS             4542..5366
                     /locus_tag="IWJ77_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP9453867.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5372..6178
                     /locus_tag="IWJ77_002292"
     CDS             5372..6178
                     /locus_tag="IWJ77_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9453868.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6175..7005
                     /locus_tag="IWJ77_002293"
     CDS             6175..7005
                     /locus_tag="IWJ77_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9453869.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7012..8043
                     /locus_tag="IWJ77_002294"
     CDS             7012..8043
                     /locus_tag="IWJ77_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453870.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8150..9423)
                     /locus_tag="IWJ77_002295"
                     /pseudo
     CDS             complement(8150..9423)
                     /locus_tag="IWJ77_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9700..9909
                     /locus_tag="IWJ77_002296"
     CDS             9700..9909
                     /locus_tag="IWJ77_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453871.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9959..10675
                     /locus_tag="IWJ77_002297"
     CDS             9959..10675
                     /locus_tag="IWJ77_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP9453872.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10577..11097)
                     /locus_tag="IWJ77_002298"
                     /pseudo
     CDS             complement(10577..11097)
                     /locus_tag="IWJ77_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3199 a         2158 c         2612 g         3389 t
ORIGIN      
        1 aaagaaaaga ctgtagacat catccataaa atggactttg tctacagtct ggataacgtg
       61 attttttgct gtaatgacaa ggttttagct gtttcagata ccatgtatcc aaaaatagtt
      121 tattgagaaa ataggagagt atttgatact aactagtagg aaaaaatgag ataaaaagta
      181 aatggaatca tttaaggtaa tctgaaataa aaaatataca gccctgtggt actttaccta
      241 caaggctgta tatatttttt actctcagcg tttttgactc cgtgtctgac tccgttttat
      301 tgattttgaa tgttattcaa tgaccattta acaggtcaaa tttgatgttt ttgattccgt
      361 atgacgccgt ttgatatcta gtgatatagt gatttgagtc tacatcgaat aagggaataa
      421 accaataaaa aatgatttag aatattaaaa taacaaaaaa gaggactacc tgtattagtc
      481 ctcttttttg accagatttt gaccagtcta ttttacatcc tagtatattt atatgagcta
      541 attttatgat taaatggtaa gtttttcctt gtgtgtttta gtttaaacta gtgtgtaatt
      601 caaaaatcta tatggaagga acagtattaa gaatagaaca gggttctcta catgatggtg
      661 ctggtttacg gaccgttgtt tatttaaagg gctgcccatt acgctgtgcc tggtgttcga
      721 ttcccgaatc acaaagcaag cagattgaaa aaggatttgg ccagaccatg accgctgaag
      781 aggtaatgga tgagattgag aaggatgctg ttttttactt tcactcggat ggcggagtga
      841 ccattagcgg aggcgaggca ctggttcagg cggattttgc taaagaaatt ttgcaaaaat
      901 cgaaatacat tggaatcaat accgtactgg aaaccagctt ttgtggtgct tataatgaaa
      961 tccaaaaggt agctccctat gtggatacat tgtttgtgga tgtaaaaatg tttaccagca
     1021 agctccacaa acaatggacc ggcttagata accaacaaat tttgaagaat attcgtcgct
     1081 ttttaattga gtatcctaac tgcgaagtgc gaattcgagt tcctgtggtt ccaggtatca
     1141 atatgaacct gaccgagctg ctgacgattg cttgttttgt ggcagatctt gatcggttcg
     1201 taccgttaga attactaccg taccactgct atggtatgca cggttaccaa gcattgggac
     1261 tggagtatcc gttggcagac acgccagcgc cttcacctaa agaaatgttt gcattggcag
     1321 atcaactggc tcgcacggtt cctcatttgc tggtcacagc tttaaccaaa acatttattt
     1381 atttattgaa tccaagcttt atcttatgga taaatgaaag acactcacta gctgcagcca
     1441 gctcaagaat agagcttttg ctgaagtcag tgagtgtctt tttacgatgt tttttcgaag
     1501 ttaatttgtc gaatgaacac tggctcaatc ggtacatctt cactgtttgt cggctgagct
     1561 gcgatcgctg ctacgatttc taacccttgg tatacctgtc caaaaacagt ataacgacca
     1621 tccagctccg gtagtcctcc aagctccaag tatgcttgct ttacattagc aggtcagcct
     1681 gcgcctttgt gttttgcaca ataaaaaact ggttgccgtt ggtgtggggg ccgtggtccc
     1741 ctaaggccaa ggcgccatta aaatgataaa gtccgcgatg cacttcatca gcgaaaaagc
     1801 cctcatagct ggaaacagct tcatcaccag aaccatccaa agcgccacct tgaataacaa
     1861 attccttaat gacacgagcc gaaaggtgtg ttgtcataaa cgccttgctc agctagggag
     1921 cgccagttgg ctacagccag gggagcttgg tcaggaaata agcggagacg aatttcacca
     1981 gcatcggtga ccagcacaca aacttcttcc tgctgcggtt tttctaattg aaagagagga
     2041 aggtttgcaa ctgaaacgcc ttgatcctgc catcaaatat cgaaaatccg ccactcgacc
     2101 ttcgcctgat caccaaggcg agccattccc aataaaacgg tataggcggt ttcatccgct
     2161 tgaaaggtca attgcagctg tcctcgtaaa tcgttgtagt catctggggc ggctacttga
     2221 taaaaaagat gaaactgcgc atgcaaatat tccaaaggct gatctttaaa ggctgcaaaa
     2281 taagcctgaa ctttttcccg ctgcaagctg tcagccacca aaaaattttc cagttgttgt
     2341 tttggaacgg ctgctaacaa ggatcggcaa accgcctcaa ttttcaacgc atcccaatgt
     2401 cgcgcaatta aataatggat gggtgcctct tgattgtagt cccacaggtt gctcattttt
     2461 ttcaccactt tcttctatta tataggaatc acaaaatcgg cagcggcgtc aggcggcgtt
     2521 cttttccttg aatgactagc aacttttcta cctgacagtc tgccaacagc tgactcattt
     2581 ttttaaagcc cagctgataa tcttcggcaa catgagcatc cgagcctaag gtaaagagct
     2641 ttccgccgac actttgatac aaaggaacgg cataacggta aaggtcttcg ttgccgtatt
     2701 tcaagaagct cttggcattt aactccatcg ccaattctcg ttcaacaacc tttttgaaaa
     2761 tcgttagcag ctgagcctca aaatgctccg cgaattcttc cgctgtgaag gtgaagcggc
     2821 gtaatccata atcaaagtga gtcaaaatat ctccttctgg aaaatccgtc aagacctgca
     2881 acatctgatc aaaatattcc ttggtagttt gaatgggatc tttggttaaa accacatcgt
     2941 ccatataatc aaagcggcca ttttgatgaa tacttaccag ctttaaatca taaggatgct
     3001 gagcgaggta atccacgatc cgctcttctt gtcctggaac aacgccaatc tcgatacctc
     3061 gcagaaattt ggtggaatag gtttctttta aagagtccag tttgtttata taggctggat
     3121 aatccggaat gtcatctttt ccggtagatg gattgtttaa atcaaagtga tccgtagaaa
     3181 cgaaaaactc cggctgatag gctaaatagt tttcaaattt ttcctctgaa tcaaaggaaa
     3241 aatacgtgtg gagatgctga tcataatact gcatagggag cctccttttt tctactatca
     3301 taccgcaaaa gctttagaag gttaacggga attcgtcgaa atttacaaac catatacaac
     3361 ctttacattg ttcatgaaac cttttcaaga aactgattct ctcattacaa agctttgtta
     3421 gaatggaatt atcgaaaggg gagaagaatg aatgaaaatg aatgaaaaaa ttcgtaacat
     3481 gactatcggt ttattttgtg cagcaacagt aagtatcctg ctgacaggct gcggctcagg
     3541 tgaagcaacg aagcaatcgg ctgcggcaga cgactcttta gatacgatta tagaaaaagc
     3601 caaggaagaa ggagagattg ccagcttggg aatgccggat acttgggcaa attgggtagg
     3661 cacttggaac gaccttgaaa aagaatacgg cttaagtcac gtggatacgg atatgtccag
     3721 tgcagaagaa ttggctaagt ttgaatcaga aggaaaaaat ggaaccgccg atattggtga
     3781 tgtcggtatc agcttcggtc cgttggctga aagcaaagag ttaaccttac cttataagac
     3841 caactattgg gaggaaattc cggattgggc aaaggatgat gatggggatt ggttgttaag
     3901 ctatacggga accatttcct ttatcaccga taaagagaat gtgaagcaag caccgactag
     3961 ctggaaggag ctgcaagagg gtgactatca agttagcgtg ggggatgtga cgattgccaa
     4021 ccaagcacaa tttgctgtat tggctgcggc gattgccaac ggaggtgacg aaaaggatat
     4081 tcagccaggt ctgaattact acaaaaaaat ggcagaaaaa ggccgtttgt ccaccgttga
     4141 tgccagcgta gcaaatcttg aaaaaggtga aatcgatgtg gcagttgttt gggacttcaa
     4201 tggactaaat tatcgtgacc aaattgactc cgagcgtttt gaggtaactg tgccaacaga
     4261 cggctccatc attagcgggt atacaacctt aatcaataaa aatgcccctc atcccaatgc
     4321 ggcaaagcta gcacgggaat atattttatc tgatgaaggg caaatcaatt tggcaaaggg
     4381 ctatgcgcga cctattcgca aggtagagct gcctaaagaa gtagctgaaa aattactgcc
     4441 ggaggatgct tatgaagcgg cggtgccagt tgaagacaat gaagcttgga acaagacctc
     4501 tgtagagctt ccagagcttt ggcaggcgga ggtactgacc tatgcaaagt agacttgaca
     4561 aactgattgt agttatacta gacggctgtc gctacgacac agccgtcgcc cagcttgggt
     4621 tcatgaatca tttagttgag caccagcaag caagcttatt gaaggtaaaa agtgagctgc
     4681 ccagcaattc acgtccgtta tacgaagtac tgatgaccgg cgttccagcc tatgaaaatg
     4741 gcatctatag taattaccat gctcagcgat ccaaagagtg gtcgctgttt gaattagtta
     4801 aggaagctgg cggaaaaaca ggagccgccg cttaccactg gtacagcgag ttgtacaatc
     4861 aagcccccta taatcgtttg gttgatcgca tccagcatca ggaggagcga ctgattcagc
     4921 atggcatctt ttatagtgag gacacttacc ctgattcgca tttatttgcg gatgcccatt
     4981 atttagtgaa tcagtatcag ccagactttt tggtcgtaca ttcgatgaat attgatgatg
     5041 tcggtcacaa atttaccgcc gattctcatg aatatgcagc agcggttaat caggcggaca
     5101 gattattggc tatttacctt cctgaatggc tgcaaaaagg ttatcaagtc gtggtgactg
     5161 cggatcacgg gatggatgcg cacggactgc atggcggcag cttggctgca catcgtgaag
     5221 ttccttttta tttgatttca aataaacgcc aaaagctgaa ggaacaaact gtaccacagc
     5281 tgctggcggc tccattgttt tgctggttat taggaattgc accgtcagag aaaatgcagg
     5341 acattcaaga attgatgagg ggatgaaaga gatgaaaaaa cgcttggttc tggttcctgc
     5401 ctcctttatc ttctgtgcat ttttactgct gccgctgctt tttatggttc tgtgcagctt
     5461 gcacagtgat ttaactaacc agtggacatt ggccaattac agtgatattt tcagcaacaa
     5521 atattacaca caggcctttg ttaacagcgt actgattgct gtcagctcca gtgtgattgg
     5581 gttaatcggg acgtttattt tatgtttatg cttaatgaat ctatcgacta aattgcagga
     5641 gaaaatcacc tttgtttcca atttggcagc taattttgct gggattccgc tggcattctc
     5701 ttttattatt ttgttgggga atgtaggtgt gctgaaaatg attttgccat ttctagcgga
     5761 cttcaatttg tattcttggt ggggattgac catcacgtat acctatttcc aaattccgtt
     5821 aggggtcttg tttttgtatc ccagcatgaa ggaagtgaaa aaggaatggc tggagtccgc
     5881 acaattgctg ggcgcttcat cagcgtatgc ttggttcaag gtggctttgc cttttttgcg
     5941 accatcgatt gccagcacct ttgtcatctt atttgctaat ggcatgggga cctatgaaac
     6001 agcgtatgcc ctgaccagca gtaatgtaaa tcttttaacg attcgaattg cggctttagt
     6061 gtcgggagat gtgttcgcca agcctaattt aggcagtgcg ttggcagtcg ctttaggtgt
     6121 cctgctggta acggcaatgt tgattattca aaagaaaggc aaggtggtgc agacgtgaaa
     6181 aagttaataa aaccgatttt gttctgcttt gtcctgtatt acttgctgcc gattattgga
     6241 acgattttgt atgccagctc cactaaatgg tcaaaatcgc ttttacctag tgattttacc
     6301 ttgcaatggt tccaacagct gctgacggac agggaattta ttgctgcggt gggtcgctca
     6361 cttttattag caggtgtggt cttggtgacg attctgttgt tgatgattcc caccattatt
     6421 tggattcatt tgtatttccc tcgtttgaat cgttggctgg aaaagctgct cctgctgccg
     6481 tatgctttac caggagtgat tttggtaacg gctttgcttc gaacgtatgc tgaaactggt
     6541 atcccaatgt ttgtagtgtt ggtaggtgca ttgtttatta cggcgttgcc gattgtctat
     6601 cttagtctta acaaccagat gcgcttaatt aatctgaagg aattagtgga tgcagcagaa
     6661 accttgggcg ccccgatgtc tacgattatt atccaggtgc tgtttccaaa catccgtatc
     6721 ggtgtgaccc tagtttctct gatgattttt tcttcggttt ttggggagta catgctgacg
     6781 aacctgttga ttggcggtcg atttcagacg gtacgcatct atatgctgcg gcggatgaac
     6841 gaaaacggcc atttggctag cgctgtgatg gttttgtatt tgctgtttat ggtgggaatt
     6901 gctttaacta cctttatgct gacttatcga caaaagcaac agctgtccct taagcagacg
     6961 agggaagaga agcagtcgaa aaagaagccc ttaaaggagt tgtgacggaa aatgttttta
     7021 gaaatacatg atctcaatct gttttttgat gaaaaacaga ttttagctat tgatgaaatt
     7081 cagattgaaa agggtgagct ggttacttta ctaggtccca gcgggtgcgg aaagtctacg
     7141 ttgctgcgct gtatcacggg attggaacaa ccaaagagcg gtgagattgt gctggacaat
     7201 gagaatattg ccgataagcc tacgaaggat cgcaatatcg gctttgtttt tcaacagtat
     7261 gcattgtttc caactatgac tgtttttgaa aatgtagcct ttggtttgaa ggtaaagaag
     7321 ctgagtcctg aggtgattca ggaaaaggtt tttgagatgc tatcattggt ggatatgacg
     7381 gagcaggcag ataaaaatgt ccagtttctt tctggcggtc aacgacaacg ggtggcactg
     7441 gctcgctcat tggtcactga accgaaagtc ctgctgctgg atgagccatt aagtgctttg
     7501 gatgcccgaa ttagaaaaca gctgcagcgg gatttgcgcg ctatccagca atcgctaggg
     7561 atgacgatga tttttgtgac tcatgaccaa gaagaggcga tgcgaatcag tgatcggatt
     7621 tttgtgatgg aagctgggcg ggtggctcag gtttccacct cgaaggaatt gtatcagaat
     7681 cctcagagcc gatttgttgc ggaatttatc ggcaactata atcgtttcga atggtatgag
     7741 ctgaatcagt atacccacga gttttccgct aagggcaatc gctgcattta ttatttaaga
     7801 ccggagctga ttcaatttga accggttcct cagggggtta agattccggt tctatggaag
     7861 gaatcattta ttttaggcaa cattcaacgg tatgtttttc aaacgctgga gggcaaagaa
     7921 attttagtgg atcggttgaa cgatcgggaa gagaaaatta gcgatgcctt atacattcag
     7981 ctgaaagata ttctagcaat ccctgtggaa attgatactc agcattttgc acaacaaagc
     8041 tagtgtatag tcggcaaaca tttctggctc tttgtcaaat aggactgata gagtgatata
     8101 agaacatcta aggtagaatt gattttctgc tttagatgtt tttttatgtt taataagtga
     8161 tcagatgatt gattaagaaa attctggttc tcatcctaac ccgtaagcta ttcgtttcaa
     8221 tgctttgatg tgggtatgca gattttctat tttgccattt gagtaaggca actctaaggc
     8281 taaacgaatg ccggattcat agtttataaa cgcttttttt atatgatgca gttctcttgg
     8341 aaccgacttg ggcattgttt ttacaagctg aaagaagatg tccgcttctt tgtcataaaa
     8401 agcttgaaga atttcttgat agtaagcata agctattcgt agttcatcag aagtagaaag
     8461 tagacgttct acgacttcac tttcggttaa ataggcccaa ccaaaattac gccattttgt
     8521 ccatttatca taaacaagcg tgtcctgtcg tttgatcaga agtcgagata aagccttcac
     8581 ttgtcgatag tgtttggctt gtattggatc gtttcggtcg aagcttttca tgactcatag
     8641 tcaaatatcc ttaaaactgc gaatgaggtg cttgacgatg tgaaagcgat ccacaacgag
     8701 tttggcatga ggaaagcact cttttatcag caaagcataa gaggcgttca tatctgttac
     8761 gagtattttt acgtttaaac gtgcacgtcg ttcaaattgc ataaaataag aaatgagatc
     8821 tttcttctta cgtgaaggaa gaatatccag taatttccca gtatcgccat ccatacaaga
     8881 aaaagaatag ggaccgactt gatttttcaa tgaacgaaat tcatctacca atgaacagcg
     8941 aggcaaccaa tttattttca cgtttgtcat ggggagcaga gaagcgagtg ttcgcgaaac
     9001 ggttgaccaa gaaacgccgc aatgtttaga aatcgtcgtc attgcttgtt tttcagtcaa
     9061 ttcctccaat attttataat gtacttgttt ggcgataaaa cagttttttt caacaatgta
     9121 ggtattcgcc gtaaaatagg tgtgacagcc acgacagagg taccgttgct ttgccaaagc
     9181 aagataagtc cgttgattcg cacacttatt gagaaggacc agcgattttt tctttccgtt
     9241 cttaacgatc agcccatgtt gattcacctg tttacactga ggacagggct gaatcgtttt
     9301 aatgagcgtg gcagaaacaa cgatactgtt gattgaatgt atttttttct tctcgacatg
     9361 agtgatttga atgtttccat ctttgattcc tagtaatagt ttgataagat gaagatcagg
     9421 catacagaac gctcctttga ttgtgttgtg gtgattcaat tctaacggag cttctgtatg
     9481 ttttgttttt ttatgctcaa aaggactgat agaatgattt actatcagtc ctagaaatta
     9541 tagcgccaca tttctttcac ctttgacttt tcagacggca gtttttatgc tgatactgta
     9601 gagagagcag gacacagctt ttgcttgttt tgggtaaggg ggaaagaaga tgaccaaagc
     9661 acttattttt gattttgatg ggacgatgat tgacagtgaa tggatgtttt acgagagtat
     9721 tcatcactat ttactgaagc ctatttactg gagcagttcg gctataaaat tccttttgca
     9781 gactatcaac aaggtgttgg gacggcagca gaactgtcct tttatgaagg gataagtcaa
     9841 gcggttggga gaccagtgga tgctgaacat tttgagcagc ggatcagcag cactggaaaa
     9901 tcggagtagt ctcaattctg aactgcggga gctagactat ttttttgaag cacatccgat
     9961 gattaaggaa aaaattgatt cctttgtgac gatcgatgat cttcaggtcg gcaaaccaga
    10021 tccagaaggt tatttgttgt gcttgaaacg gctgagggtt gaacaggctg ttgtagttga
    10081 ggatagtcca gtcggtgccc aagcagcacc gcatatgaca gttgatgtac aagcgttgga
    10141 tgcagatttt ttcgccttta gcgggcataa aatgtgcggg ccgacaggaa tcggtgtatt
    10201 atatggcaaa cgtcaattgt tagaacaaat ggagccagtc gaattcggcg gtgaaatgat
    10261 agattttgtc aatctttacg atagttcatg gaaagagctg ccatggaaat ttgaagctgg
    10321 tactccgaac attgcaggag caatcgatta cttgaatgag attggtatgg agaatattca
    10381 tcgttatgag caagaactgg ttgcctacgt tttacctaaa ttacaagcga tcggcggtat
    10441 caccatttac ggaccgcaag atcctgaaca tcatactggc gtggttgctt ttaatttgga
    10501 tggcatccat ccccgtgatg tggcgactgc tctggatatg gaaggtgtcg ctgtacgttc
    10561 aggacaccat tgtgcacaac ggttcatcga cgatttctgt ttgactaatt ggtcgtttaa
    10621 acagtcgatg ataatgccgt ttgagcggct ccaatgtcgt ggaatctttg agtaagagtt
    10681 tccaatatcg cttgagccga cggtacttct tctgattttc agattacgtg attgattttc
    10741 gtacagttct atgagaatcg cctgcttcag ttcctttgtt agttgatgat gttcatcaac
    10801 taagtccgtt ttcgcagaaa aggtctgccc acaatcatag catagaaaac gggtacgttt
    10861 taattctaaa atagtcaact tttctttgac tttcaacatc tgagtacgtg tcgtatagga
    10921 accgttgcgg ataatacgaa tcgatccaca gtgttcacaa tgggaaggtg agtcatccaa
    10981 tctcccttta ataaactgcg cttgaacgcc tttatattct ttttcaatca gccattcttc
    11041 atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt ctagtatgat tatccataga
    11101 gcgactcctt tggtagggtg atgttttggt cgactaaatt ttaccaaaag tgagtctctt
    11161 tttgagttga aacgaagaaa aagtgttagt agaaaatcgc cttcgatttt ccaacaacac
    11221 aaaatattat agaagccttt tttctaaagg atttcaaggt gtctgtttgc atttattaaa
    11281 aaaaagaaaa agtattggca acagaaggcg tcaccaacac tagatattat agagccataa
    11341 tatgctgtat aatatcta
//
LOCUS       DACDRK010000080        11179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDRK010000080 DACDRK010000000
VERSION     DACDRK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.080"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            206..1441
                     /locus_tag="IWJ77_002299"
     CDS             206..1441
                     /locus_tag="IWJ77_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9453873.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2440..2700)
                     /locus_tag="IWJ77_002300"
     CDS             complement(2440..2700)
                     /locus_tag="IWJ77_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9453874.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2727..4028)
                     /locus_tag="IWJ77_002301"
     CDS             complement(2727..4028)
                     /locus_tag="IWJ77_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9453875.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4320..4778)
                     /locus_tag="IWJ77_002302"
     CDS             complement(4320..4778)
                     /locus_tag="IWJ77_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9453876.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4791..6116)
                     /locus_tag="IWJ77_002303"
     CDS             complement(4791..6116)
                     /locus_tag="IWJ77_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9453877.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6133..7545)
                     /locus_tag="IWJ77_002304"
     CDS             complement(6133..7545)
                     /locus_tag="IWJ77_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9453878.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7562..8326)
                     /locus_tag="IWJ77_002305"
     CDS             complement(7562..8326)
                     /locus_tag="IWJ77_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9453879.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8430..9305)
                     /locus_tag="IWJ77_002306"
     CDS             complement(8430..9305)
                     /locus_tag="IWJ77_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9453880.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9560..9644)
                     /locus_tag="IWJ77_002307"
     tRNA            complement(9560..9644)
                     /locus_tag="IWJ77_002307"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9608..9610),aa:Leu,seq:caa)
     gene            complement(9662..9733)
                     /locus_tag="IWJ77_002308"
     tRNA            complement(9662..9733)
                     /locus_tag="IWJ77_002308"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9699..9701),aa:Gln,seq:ttg)
     gene            complement(9743..9815)
                     /locus_tag="IWJ77_002309"
     tRNA            complement(9743..9815)
                     /locus_tag="IWJ77_002309"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9780..9782),aa:His,seq:gtg)
     gene            complement(9877..9961)
                     /locus_tag="IWJ77_002310"
     tRNA            complement(9877..9961)
                     /locus_tag="IWJ77_002310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9925..9927),aa:Leu,seq:caa)
     gene            complement(9982..10052)
                     /locus_tag="IWJ77_002311"
     tRNA            complement(9982..10052)
                     /locus_tag="IWJ77_002311"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10018..10020),aa:Cys,seq:gca)
     gene            complement(10098..10169)
                     /locus_tag="IWJ77_002312"
     tRNA            complement(10098..10169)
                     /locus_tag="IWJ77_002312"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10135..10137),aa:Gln,seq:ttg)
     gene            complement(10181..10253)
                     /locus_tag="IWJ77_002313"
     tRNA            complement(10181..10253)
                     /locus_tag="IWJ77_002313"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10218..10220),aa:His,seq:gtg)
     gene            complement(10256..10329)
                     /locus_tag="IWJ77_002314"
     tRNA            complement(10256..10329)
                     /locus_tag="IWJ77_002314"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10295..10297),aa:Trp,seq:cca)
     gene            complement(10336..10416)
                     /locus_tag="IWJ77_002315"
     tRNA            complement(10336..10416)
                     /locus_tag="IWJ77_002315"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10380..10382),aa:Tyr,seq:gta)
     gene            complement(10418..10493)
                     /locus_tag="IWJ77_002316"
     tRNA            complement(10418..10493)
                     /locus_tag="IWJ77_002316"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10458..10460),aa:Phe,seq:gaa)
     gene            complement(10529..10601)
                     /locus_tag="IWJ77_002317"
     tRNA            complement(10529..10601)
                     /locus_tag="IWJ77_002317"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10566..10568),aa:Asp,seq:gtc)
     gene            complement(10611..10683)
                     /locus_tag="IWJ77_002318"
     tRNA            complement(10611..10683)
                     /locus_tag="IWJ77_002318"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10648..10650),aa:Val,seq:tac)
     gene            complement(10699..10770)
                     /locus_tag="IWJ77_002319"
     tRNA            complement(10699..10770)
                     /locus_tag="IWJ77_002319"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10735..10737),aa:Glu,seq:ttc)
     gene            complement(10785..10874)
                     /locus_tag="IWJ77_002320"
     tRNA            complement(10785..10874)
                     /locus_tag="IWJ77_002320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10838..10840),aa:Ser,seq:gga)
     gene            complement(10914..10987)
                     /locus_tag="IWJ77_002321"
     tRNA            complement(10914..10987)
                     /locus_tag="IWJ77_002321"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10951..10953),aa:Asn,seq:gtt)
     gene            complement(10997..11112)
                     /gene="rrf"
                     /locus_tag="IWJ77_002322"
     rRNA            complement(10997..11112)
                     /gene="rrf"
                     /locus_tag="IWJ77_002322"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3558 a         2412 c         1813 g         3396 t
ORIGIN      
        1 acaacatata aatcgatcaa aaaattgttg cgtgtttttt tgaaaattgg agtttcaact
       61 tgattacaaa atttaaattt agaagttgtg aaaacagcga gagacagttt aatattcaaa
      121 atatacatag gctactcgaa cgctgtgttt tttagtatac tagtaagaaa cgaagggata
      181 gactcattcc aataaggagg atttcatgaa gacaattctt tttgctcctg cagtattcaa
      241 tttagctgag acaacacgaa tgattgaggt tgccaaagct atgaaagacc gttattactg
      301 tgtattcttt ggcttttcta aaacttttgc tcctttgatc acagaaagtg gctttgagta
      361 tcatttacta gcgccgatcc ttacgaaaaa acaagaagaa caaatcataa aatttgacca
      421 gttgcgtaca cttaaaaacc cttttaccaa agaaatggtt gcctctcgta tcactagtga
      481 acagagtctg attgctaagt ttgagcctcg actgattgtg acaggttcga atgtgacaat
      541 ttttttatcg gcccgcaata aaaagattcc tttagtatat gttaaaccct atgctttgag
      601 tgaacctttt tttcaatcag agcatgcgct gatgccccac gagttacgtt tcttcggccc
      661 tttaagcaat cccctttggc actggacaaa aaagcgtata gtaaataccc gatggatccc
      721 caatgccttc aaacacgctg cacaaaaaga aatgcttcct ttaccaaaaa caagtttaca
      781 aatgatggat gctgatctaa atctcatcac aacgcctaaa ctatttttgg aagaagaaca
      841 gctgccggaa aattatcgaa tcgttggtcc aatttttgct aagctcagta cttccttgcc
      901 tgaagaagtg ctaactttta tacagcgaaa gcaacaagaa agaaaacgta ttatctattt
      961 cgcaatgggt agttcaggca atccgcgtat gttacgcaaa attttgttct tcttacaaaa
     1021 acaaccggat ctagcggtta ttgcacctat tgactatctg cttgatgaca ttcaatctga
     1081 ctcagaagat cttttaataa cagaatatct gtctagtcat cttttgcatg aattcattga
     1141 tttttctttt atccacggcg gggaagggac cgtccaaact gcctgtgaat ccggcaaacc
     1201 gtttatcggt gtggggatgc aatttgagca aagctgtaat attgactatt gtgtaaaata
     1261 cggaaacgca atcgccctca caaaaccagt gactgaaaga aaactgcaga ttgctctgcg
     1321 cgaagtttct tcacccaaga ttcgtcagca ggcacgcacg ctgcaagccg tttttccaat
     1381 aaatggtcca caacaagcaa ttcaagaaat cgaaaccttc ctgagcaacc acccaatata
     1441 gtttcaagta gaaaaagctt ttttaataga gactcatcag taatttcgct attcgagacc
     1501 cagacagtga aaatacaaaa aaagtaaata gtggattgaa aagttagagt tacttaaatt
     1561 acggtgtcta aaaaacacaa aatcatcctt aaaataaaag atctgacgct atccaaatga
     1621 gcataggtag cgtctgccat ttattcttag tcaaattctt tgacaccttt ctctggttgg
     1681 atcatctcaa cgtttcttca aaagaaagac cataataatt aggcaaatac attttcttaa
     1741 aatgtgtcat atagatctag aagcatcgct ttgtagagat ttgtgccgct agtcccctca
     1801 acaattacta aatcaaatgt aggatttacc actttatctt tgataatcaa cctccctctt
     1861 ctgttgagac aatgatctcg caatccgatg catactccta tataaactgc cagcaatccc
     1921 cacatattga gtagatcatg tcagaatgtt ctatgtcgtc agcaacgata cactaaaagt
     1981 cacgttcacc atgactgatt gctattccca gcacaattat ttctatacac gtttcatgat
     2041 ttgaataaac actcacatca tacatattac cattcataca ccatactgca gcccctacta
     2101 tatggttgta agctcccaaa tctatatttg acttgatcac tttggtagta gatctactaa
     2161 ttctaaatcc attgctgata aaatttccat tttttcctcc ttctttctgc caaaacagtt
     2221 aaaacaataa taatctttat gtaaaaaagg gctgtgacaa aagccttgtc acagtcccat
     2281 attccgaata aacggtggtc aaaagcttgc atctgcggta ataagttcaa ccaaacgaaa
     2341 aataagccaa catttcacaa aaatttaaga agcaattttc gtaaatttct tcttatttct
     2401 tgaagctaac cacttctgtt acaaccactt ttagaacatt taaagcttcg atgtgacaaa
     2461 ccatctatgc tgctcgatca taaaattgat agcaaaaatt ccacaaagta catatgtccc
     2521 ccatgaaaac aaaacattca tatgcggtac aatgatcttt ggaagaatca cgatcaaaaa
     2581 tgatgccagt gcccaaatcc atcgattgat ttgaatatga tatgtagcta gcatattcaa
     2641 tataaggtga ctagccgtta ataaaaagat aaaaacaaaa aataaaatca gccaatacat
     2701 tgtttcccac cttctaaaaa ccaaacttag acagtctgga cttcgttttc tccacgactt
     2761 tgttcatctg acagcatctt tttatcataa gcttttacaa atggataata gataataaat
     2821 gctatcaatg tgttgatcat gatcaagata acaacacgcc agtcatttcc tgctgaaaga
     2881 taaccgccga ttggagctgg taatgtccat gttggcaaaa cagtaaatct attgaccatt
     2941 tctaatttga ctgcaaaata actgataata gtcaaaacta aaggaactaa gttgaacggg
     3001 atgaacatgt aaggattcaa catcatagga gctccaaaaa ttatgggctc attgatattg
     3061 aagataccag gaataataga aaaacgacca acctctttca agaattttga tttacagaag
     3121 aatataaaca aaacagatac cacgatcgtt gctcctgcac caccaattgt taccgtccat
     3181 tgataaaact gttcaggagc gatatagggt aattgggtgg cagctgtacc atctgccaat
     3241 gcctttgcat tctcatccaa catgatcagc cacattggac gaacaacagc ccctacaata
     3301 ctcatgccat ggataccaaa tgaccacaac aaatggatca aaaacattgg tagtaaaaca
     3361 ccaagcaaat tattccctaa aatattggtt aaaggctcga ataacgataa taaggcagcg
     3421 tttacatcaa agcccgcgac gcttcgaatc agccaagtaa ttgttaaaat gactgcacct
     3481 ggaatcaatg cctcaaaact acgggcaaca gcaggtggca cttgttctgg cattttgata
     3541 gtaatatttc gatttttaaa gaaacgatat acttccactg caaaaatcat agataagatc
     3601 cccgcaaaca tcccagatgc tcccaaagaa ctcataggta agacccagcc aagaggtgcc
     3661 tcttcaggag ttaaaacgtt tacaggaact tgtaacataa agaacgttcc taaagataat
     3721 ataccacctg tcacactatc taatccatat gatttagcta gatccgaacc cataacaaaa
     3781 gttgcataaa tagccatcaa tcccatcgtc atcctaaagg gtaaaacaat cgttgccgaa
     3841 taaggggcaa taaattcatt ccatccagga aacggtaaat tgataaagac cataaaaaat
     3901 gtcccaatca aaatcaatga caatgttgaa acaaccccat tacgaattgc taataaatga
     3961 cgctgtgtac caatttttgc cattggtggg acaagtttct cttcgaccca tttcatcagc
     4021 ttttccacta aaaccactcc ttttcaataa aatatgaacc attcattgca tgacaacgca
     4081 aagaatgttc ataactaatt atataattaa gaaattttat ttcaatatat tctcttaaat
     4141 aatcaaattt atttttaaga atcctgaaaa taagttttct aaatcatagg agcaacaaaa
     4201 aaattcgata attaacaaaa atcaaaatga ttaaatctag tgattgaaac atatcatttc
     4261 aaaaaaattt tgtaaattca cccataaata tcaccctatt taactttttt catcgtacac
     4321 taaaattcaa aacaactatc tttaatcttt acttcttggt tttgttttaa taaatttcct
     4381 tcaaaaatgc ctggagaaac tacaatgatc accgtatctt tttgtgcttg ctccaacttc
     4441 ttcaaatcta taatagctaa cagtgtccct tcttttacct cctggttact ttcaacccgt
     4501 atctgaaacg gacttccttc gagttcaact gtatctaaac ccatatggat cagtattttt
     4561 ctgccagatt cactttcgat cgttatggca tgtcgggtag gaaatatatt atcgattatt
     4621 cctttaactg gcgcatggat atatccatca tgattcgtta tagcgtatcc aactcctatc
     4681 atttttgaag aaaagacctc gtcttttact gctgttaatg gaataatttg accactagct
     4741 acagctttta gaatgtttct tgttttaaag aaatgcactt tttatcacct ctactttaat
     4801 gctggccaat attttttgtt tactttgatc atttcatcta gtattttttt tgcgactctg
     4861 gcatttggta cggtttgatt caatgtaaat gcttgtaaag ctttttgata agaaccttca
     4921 aagtaagcat ctactagtaa cttctctact gcaacttgtg cctccatcaa tcctttatga
     4981 aaatcactga ttggttttct tagtgtaatt ggctccacac cagttgcacc aacatatgcc
     5041 ggtatttcaa caactgcatc tgaccgtaga ttcggaatcg ccccttgatt aggaacgatt
     5101 agcataaagc gacgacgatc atcattcaac aaggaaatag ctatatcgac gatatattgt
     5161 ccatgctccc caaaattaaa gttcaatact tcttccacaa taccgcggcg tattttatca
     5221 gccatttctt tggtattttt ctctcgtcca tccatgacca tattagcacg tgtatactct
     5281 ttgtcagtat gctcaacgac catatcagga tataagtaat actctaagta attattcggg
     5341 atattacttg gaaaattttt cgtgatgact gacatcattt gaaaggtttt ttgccaagtt
     5401 ttatctccta tattaaaatc ggcgacttgc atttcttttg ttattagttt ttcaataatc
     5461 tctggcataa tttctcgttg caattcttta tcataaatcg atgtatacca gccaaagtgg
     5521 tttaaaccat aatacgtaac gatccaattc ttacgatcat aaccgtaatt cactgcaatc
     5581 gtttcttcaa ttgaaatagt catatcacag gcattaatca tttttacatt cggatattta
     5641 cgacgaacag cttcagaaac gatcgactct ggatttgtat aattcaaaat ccatgctttg
     5701 ggggcatagt cttgtatatg atcgacaatt tccaataaac ctttgattga gcgcatccca
     5761 taagcaaatc ctccgagtcc acacgtttct tgtcctacta atccatgttt aagtggaatc
     5821 ttttcgtcct tttctcgcat ctccaatcct ccaacacgaa tttgtgagaa aacaaaatcg
     5881 catcctgtaa aagcaagctt tggatcatcc gttgcacgaa tgcgaatatt tgaaaaacct
     5941 tttcttttca gcataaattt aacaatcaag tacatatcca tatttctttc ttcatcaata
     6001 tcatataaac gtatttcaga taatggcaac cgtttttcat tgttcaaaat ggcttgaacg
     6061 attcctggtg tatacgtact cccgcctcca gctatagtga taatctgttt tttcatcatc
     6121 atcttttcct ttctattctt ctaataattt attgaattct gccataacat aagtcacttc
     6181 taagccgatg ataatttgat aatttttttc agttggttta atgatcccgc ttatccctaa
     6241 ttgcttgatt cgctgttcct cgaccatggt gggatccact acctctaccc gtaggcgtgt
     6301 attacaacta tagacatcca ggatattggc ttttcctcca aacgcttgta gcatttttgt
     6361 tgccatatat tcataatttt tacttgctaa ccctaactct gtttcttcta aggttacttc
     6421 attgccaaac tctgccttag cttcacgtcc taatgtctga atgtttctct tattgatcaa
     6481 ataataaaaa gtaacgtaat acaaggcaaa aaagaaaatt ccgattggaa gaatcaaaat
     6541 agcattggta gcgattcgaa aattgatgag ataatcaaca atacatgctc cgaatgtaaa
     6601 accaattcgt atattgaata agtaacagac aatccctgct aaaccggcaa aaagtgcatg
     6661 gatcacgtac aacataggag aaagaaacat gaaactaaat tcaacaggct cagtgatatt
     6721 tgcaataaat gaagttcctg caccgcttcc catgattcct ttgaccatct ctttattttc
     6781 tttataagct gctttataga tagctagaca tatagctggt ataccaaaca tcattactac
     6841 aaaaaaaact gataaaaaca gtccagcctg aggatctccg ttgataaagc gtgtcatctc
     6901 tcctctgaaa acttccttgc taggagatgt atagcttcct aaatcgtaat aaatatacgt
     6961 attcaacaca tggtgcagtc caaaggggat cagcaaacga ttcaaaaaca taaatatccc
     7021 cacacctaat gctcccatat caacgagcag ttttgcaaaa ttatcaatcc caatttggat
     7081 gagcggccag ataaagccca aaaaaacagc tgcgattgtt tgtagaatca tcaccatagt
     7141 caatgggaat ttttcgcccg caaaataaga aaaaactact ggtaattttt gttctttaaa
     7201 gcgattatac gtccatgccg ctactaaacc ggaaataatt ccaccaaaaa tccccatatt
     7261 cacttctggg tccataattt ctaagccttt tttcaaggta gcaatcgcta aaaaaccagt
     7321 aagtgcaggt attcctttgt ccttattttt gacaaaaccg attgcaactc ctatagcaaa
     7381 taagatatcc aagtttccaa aaacggcatt tccagctgca ccaattactg ggatgttcag
     7441 catatcgtca gctgataaac gcatgatcaa tcccgctaat ggcatcgctg caactggaag
     7501 tagcatcgct cttccaagct tctgcacatt tgatttaaca ttcattttta ttcctcctct
     7561 attaaatatc ctcatatgtt actaactcaa tcaaaggatc atttttcact ttttgagcaa
     7621 atgctgaatg aattaaaaat tcgacttggt gggtacgagg atacacaaaa cttgaaccat
     7681 ttaacaattc ctgatccaaa tacgctgcgt gacacatgac ttcgatcgtt ttatcttctc
     7741 tcaatatcat gagtaaactt gccaaatatt cttccacact cgtattgatt tgtcgttttt
     7801 tttcatcagt ttctcctacg acatcgaaac aaggctcaaa ataatcgaca tgacgaactt
     7861 catcttttcg ttcaaagttg ctccgaaccg gcaaatcata tttttttgct aatgtaacaa
     7921 ctacctcctg attttcatcc aaagtatgag tatgatgatg actgtctaaa tgagttggta
     7981 tgatccccgc atgataaatt ttttgaatct gagcattcca ttcctgatac agttgatctt
     8041 tgtcaatgtt aaatggatgc tgataataag ataaggatct gaactctccg ctttcgataa
     8101 gagtatcgac agttttgagg agtggtttgc cacacgtcaa tgttaaatgg actcctacac
     8161 ctagcctagg catttccttt cttagtttca cagcatgctc gaaacccggc atatttgcca
     8221 tcaatgtagt tgatgtcaaa attcctcgct gataagcatc cataatccca taattcacac
     8281 catggctata gccaaaatca tccgaattaa aaatcacttt tcccatttca ttctccttct
     8341 tttttctact aaaataggcg caaccaaata atcatagtcc atttactatg tgtcatttgg
     8401 ttgcgcctat agatagttac gcccttattt taatagattc ttgctagatg aattgccaaa
     8461 tagatgattt cattttcttg aatggcaagt tgaaattgct gttcgatgat atctttaatc
     8521 tttagtgctg tacaatacgc tttgctatgt ttaaatgttt cagtaaaaaa accaatattt
     8581 tctactggcg tttcttcctg ataaatcaag cgtttaacca ggaatttgat atgaaccatt
     8641 aagcgatgat aagtcagtga ttctttatca taaacgtttg aataggattt ttctagatgg
     8701 gacaaaatcg tggtcgtcat ttctactatt tttaagctct ttaaactatt aggctctcct
     8761 agctcagcat taatgatatg catagtgatg aatcctactt catttgaccc taactcggtt
     8821 tgatatcgat gattgattcg ctcgaccact tctactgcta tatcatattc atttggataa
     8881 aggaatctca tttctggcaa cataattgct gtgacatctc tatcttctct ttttcgttga
     8941 atagcaaaat aaatatgatc tgtcaatgta atatagatat ttgaatctaa tggagaggag
     9001 atcctagctg ttatcagttc aatcacttct tccgcaaaat ccataaattc gattggaatc
     9061 tgcgagacta tctcactgaa tcgtttgatc ttttctttag aatctaatac aaataatttg
     9121 tcagcatcct caacatcaaa tggcttactc actttagcag aaaagccaat ccctttcccc
     9181 atcgcaattt tttctattcc atcgtcatcg atcaatacag cattatgatt cagctttttc
     9241 atcacataca ccgttaattc acctcctcta tatgataaaa caaaacaact tgcagactcc
     9301 ttcatacata tttttgtctc caaacaattt ttaaagacaa aaaacgacct aagaaagaac
     9361 cctttcttag gtcgtgccta attgaatagt agcaccctac ataatttaca aaaatagttt
     9421 aacacataat aaaagcgtta tcaatatctt ttttactcgt tttatgataa tacttgtatc
     9481 gaattgattt ggaggtaatt acataaaaac aaaaaaaata tagcaactct ctataagaaa
     9541 gttgctatat tgataattat accggcggcc ggggtcgaac cggcacgtcc tcaacggaca
     9601 ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaaattt aaaaataata
     9661 actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg ccttaccgct
     9721 tggctatacc ccaagaataa agggcgaatg atgggaatcg aacccacgaa tgccggagcc
     9781 acaatccggt gcgttaacca cttcgccaca ttcgccatca taaaagatgt tatttaattt
     9841 ctcaataaaa aactaagatg aagacatctt agtcaatacc ggcggccggg gtcgaaccgg
     9901 cacgccctca acgggcactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg
     9961 caagttgatt tattagtact aaggcggtaa ccggatttga accggtgatg aaggttttgc
    10021 agacctctgc cttaccactt ggctataccg ccattatact attatcaatc tataaaataa
    10081 agaataaatt atagaaactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt
    10141 cccttgcctt accgcttggc tataccccaa gaataaaaag ggcgaatgat gggaatcgaa
    10201 cccacgaatg ccggagccac aatccggtgc gttaaccact tcgccacatt cgccatggca
    10261 ggggcagcag gaattgaacc cacactaacg gttttggaga ccgctgttct acctttaaac
    10321 tatgccccta taaaatggag gagagtggat tcgaaccact gaaccctaag gaacggattt
    10381 acagtccgtc gcgtttagcc acttcgctac tcctccatgg tggcgcggga cagaatcgaa
    10441 ctgccgacac acggagcttc aatccgttgc tctaccaact gagctaccgc gccatatttt
    10501 aaaattatta attgaataac aagaacgacg gttccgacgg gaatcgaacc cgcgatctcc
    10561 tgcgtgacag gcaggcatgt taacccctac accacggaac cagttcatta tggaggttaa
    10621 cgggatcgaa ccgctgaccc tctgcttgta aggcagatgc tctcccagct gagctaaacc
    10681 tccaacattc aattaaaatg acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg
    10741 gcggtgtctt aaccgcttga ccaacgggcc aggtaaattt gctacggaga gtaagggatt
    10801 cgaacccttg agacagtttg gcaccatcta catgatttcc aatcatgctc cttcggcctc
    10861 tcggacaact ctccagagtg aaagaagctc aatactcttt ctcaaaatga gaactccggc
    10921 agtaggactc gaacctacga catcatgatt aacagtcatg cgctactacc aactgagcta
    10981 tgccggaata acatcagcgt ggcgacgtcc tactctcaca aggggcaacc cctcactaca
    11041 atcggcgcta agaagcttaa cttctgtgtt cggcatggtt acaggtgtat ccttctcgct
    11101 atcgccacca cactgtggtg ttatctttta ttgagtaaat tttgttcact caaaactgga
    11161 tttgaagtca cataagttt
//
LOCUS       DACDRK010000081        11176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDRK010000081 DACDRK010000000
VERSION     DACDRK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.081"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWJ77_002323"
     CDS             6..305
                     /locus_tag="IWJ77_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9453881.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWJ77_002324"
     CDS             378..1739
                     /locus_tag="IWJ77_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9453882.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWJ77_002325"
     CDS             1761..2021
                     /locus_tag="IWJ77_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453883.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWJ77_002326"
     CDS             2035..2787
                     /locus_tag="IWJ77_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9453884.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWJ77_002327"
     CDS             complement(2881..3486)
                     /locus_tag="IWJ77_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9453885.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWJ77_002328"
     CDS             complement(3539..4513)
                     /locus_tag="IWJ77_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9453886.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWJ77_002329"
     CDS             complement(4891..5670)
                     /locus_tag="IWJ77_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9453887.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWJ77_002330"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWJ77_002330"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9453888.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWJ77_002331"
     CDS             7161..8558
                     /locus_tag="IWJ77_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9453889.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWJ77_002332"
     CDS             8639..9079
                     /locus_tag="IWJ77_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9453890.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWJ77_002333"
     CDS             9227..9553
                     /locus_tag="IWJ77_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9453891.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWJ77_002334"
     CDS             9554..10303
                     /locus_tag="IWJ77_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453892.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWJ77_002335"
     CDS             complement(10383..10838)
                     /locus_tag="IWJ77_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9453893.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3551 a         1956 c         2294 g         3375 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaa
//
LOCUS       DACDRK010000082        10929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDRK010000082 DACDRK010000000
VERSION     DACDRK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.082"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..288)
                     /locus_tag="IWJ77_002336"
     CDS             complement(<1..288)
                     /locus_tag="IWJ77_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453894.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="IWJ77_002337"
     CDS             430..1548
                     /locus_tag="IWJ77_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9453895.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="IWJ77_002338"
     CDS             1676..3052
                     /locus_tag="IWJ77_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9453896.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="IWJ77_002339"
     CDS             3453..5033
                     /locus_tag="IWJ77_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9453897.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="IWJ77_002340"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="IWJ77_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="IWJ77_002341"
     CDS             complement(5659..5979)
                     /locus_tag="IWJ77_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9453898.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="IWJ77_002342"
     CDS             complement(6127..8361)
                     /locus_tag="IWJ77_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9453899.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="IWJ77_002343"
     CDS             8840..9106
                     /locus_tag="IWJ77_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9453900.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="IWJ77_002344"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="IWJ77_002344"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9453901.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2216 g         3322 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaaa
//
LOCUS       DACDRK010000083        10899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDRK010000083 DACDRK010000000
VERSION     DACDRK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.083"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWJ77_002345"
     CDS             23..646
                     /locus_tag="IWJ77_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453902.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWJ77_002346"
     CDS             1096..2145
                     /locus_tag="IWJ77_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9453903.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWJ77_002347"
     CDS             2142..3023
                     /locus_tag="IWJ77_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9453904.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWJ77_002348"
     CDS             3026..3796
                     /locus_tag="IWJ77_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9453905.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWJ77_002349"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWJ77_002349"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9453906.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWJ77_002350"
     CDS             5107..6270
                     /locus_tag="IWJ77_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9453907.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWJ77_002351"
     CDS             6387..6797
                     /locus_tag="IWJ77_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9453908.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWJ77_002352"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWJ77_002352"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9453909.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IWJ77_002353"
     CDS             9337..9654
                     /locus_tag="IWJ77_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453910.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IWJ77_002354"
     CDS             9814..10050
                     /locus_tag="IWJ77_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9453911.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IWJ77_002355"
     CDS             10047..10445
                     /locus_tag="IWJ77_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9453912.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3804 a         1804 c         2283 g         3008 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggatttt
//
LOCUS       DACDRK010000084        10585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDRK010000084 DACDRK010000000
VERSION     DACDRK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.084"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..581)
                     /locus_tag="IWJ77_002356"
     CDS             complement(<1..581)
                     /locus_tag="IWJ77_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453913.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKL"
     gene            complement(681..1190)
                     /locus_tag="IWJ77_002357"
     CDS             complement(681..1190)
                     /locus_tag="IWJ77_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9453914.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1391..2260)
                     /locus_tag="IWJ77_002358"
     CDS             complement(1391..2260)
                     /locus_tag="IWJ77_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9453915.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2280..3401)
                     /locus_tag="IWJ77_002359"
     CDS             complement(2280..3401)
                     /locus_tag="IWJ77_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9453916.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3403..3798)
                     /locus_tag="IWJ77_002360"
     CDS             complement(3403..3798)
                     /locus_tag="IWJ77_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9453917.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3817..4614)
                     /locus_tag="IWJ77_002361"
     CDS             complement(3817..4614)
                     /locus_tag="IWJ77_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9453918.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4616..5404)
                     /locus_tag="IWJ77_002362"
     CDS             complement(4616..5404)
                     /locus_tag="IWJ77_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9453919.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5418..5894)
                     /locus_tag="IWJ77_002363"
     CDS             complement(5418..5894)
                     /locus_tag="IWJ77_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9453920.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5966..8623)
                     /locus_tag="IWJ77_002364"
     CDS             complement(5966..8623)
                     /locus_tag="IWJ77_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9453921.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(8642..9046)
                     /locus_tag="IWJ77_002365"
     CDS             complement(8642..9046)
                     /locus_tag="IWJ77_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP9453922.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(9278..9535)
                     /locus_tag="IWJ77_002366"
     CDS             complement(9278..9535)
                     /locus_tag="IWJ77_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP9453923.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(9660..9992)
                     /locus_tag="IWJ77_002367"
     CDS             complement(9660..9992)
                     /locus_tag="IWJ77_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453924.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         3294 a         2147 c         1474 g         3670 t
ORIGIN      
        1 tccagtttct tagcatctcc tgactcaacc ggaattatcc aagcccagtc taaataattt
       61 tttaaaatct catcatattt tgcaaatatt tcattgccat attccgtcct taaaattata
      121 ttttcagtgc cagacaagtg atttatattg agttgataaa aaagatctcc atctaaaaaa
      181 atatcattag aagaagattt ccccacagta atgaccattc catcagctct atgtgttaca
      241 tacaaataat tgtatgacct catttcacct aatgaatcaa ttatttcttg ccctttagct
      301 ctaatatatt gatctctatt acctatctca ctcttattta acaaaagttt tttcatgtca
      361 aatttaatcg gctgttttat ttttgtaaat tcgcttctac tactaatcca atccgagatt
      421 aatttttcaa catgtcccag agtaaactct gatttattcg ctaaatactt tttttgttgt
      481 attaaagaca tctttccccg aagctcaaaa ttgagcctac atcgattctc tatctcttca
      541 aaaagcatct tatcttcatc attcttatat tcatcattca taaaaccaat ccagctcctc
      601 gattaagata atattttaat ctcatacaaa aactagctct catcaaaaca accattgttt
      661 tgatttctca tgaaatttct ttatactttt ctccgattat aaattcctaa ccccttgaga
      721 aaattacgca tatatttatc tccgcaaggc ttaaaatttc gttggccttc ttttctcaaa
      781 aatgccccga tttcgccttt tgatggatag atttctgcct cagcaaaaac gtctaaaata
      841 tcatcagtgg tcatagacaa ggcgattttt aattttttta gcaacacatt gttgatatat
      901 ttgatttcct tgcttttttg cggcgtgtct actatcggct tatctttttg cggaccacgc
      961 gccaagagaa tcaggccatt taggaatgcg tcaaaaactt ggttgttgat ggttttttca
     1021 tacacattat tttctgcatt atctttgttc ttatcctgta ctttggtcag taccgcctga
     1081 gcttcttctt tagtcagtgg tacaccgcct agctcaaaag ctttaatcaa atccgtatct
     1141 ttcaaatcaa tcgcataacg caatcgcaat aacctatcat tgttattcat ttgttcctcc
     1201 gaagtattta gtctttactt tcttacgtac tttttgtctt gctaagattt ttaggatcaa
     1261 tgataaaaac cgcatcactt atgataaggc gctgggtatc atgagtgaat gatattgctg
     1321 aatacaaata gtccgaccac attaagttga tcggacttca taagcatttt attgactaat
     1381 acttttttgc ttaagaattg attctaacat cttttgctaa agttaagcat cctatagttc
     1441 ctgcattatc tgctaactta ggtgtgacaa tgtattcttc gatatttggc gtatcgacat
     1501 agccgttaat tagttctaaa aaacgtcttt gtactttctt tttcagatgt tcttgttgca
     1561 tgacaccacc accaaaaatt ataatatctg gtgaaagcat cagggtcgta ttacaggcac
     1621 attgtgcaat ataatccgcc tcgatttccc agaacggatc ttctatagaa agatcttgtc
     1681 ccttggttcc cgtacgcatc tcgatagcgg gtccagatgc caacccttcg agacaatcat
     1741 gatgaaaagg acaagtacca gagaaagtat ctcctgaatg tctcttaact aacgtatgcc
     1801 ccatttctgg atggctaaac ccttcaacaa aaatcccatc ttgtatagct cctccaccaa
     1861 ttccagttcc aacagtgtaa taaacaacac tggataaacc ttttccatga cctgaaacat
     1921 actccccgta acaagcagca ttcacatcgg ttgtccaggc aattggaatt tcgaaatgct
     1981 tattcaacgt tccaacaaaa tcaaaatttt tccaggcagt ttttggagta gaggtgatat
     2041 gtccataggt ctttgattca cgatggatat caatcggtcc aaatgaccct actccaatgc
     2101 tttctaattg ttctttatag tgattaaaga aatcaattac caaactcatt gtttcattcg
     2161 gtgtagttgt aggaaaactc actctttcta aaacctttaa atcatcattg ccaatggcac
     2221 aaacaaattt agtcccacct gcttctattg agccatacat ttcttactcc accgtccttt
     2281 tatctaggaa ttcgttcttc cctgtctaat agtttttcga aaaactcatg tggttccttt
     2341 tggtaccaat aggcaacaga agaaatatcg tcttgacgct cataaagctt gatgtcatca
     2401 tttcctaggg tttggaaagt aacacgtaaa ttttctttga aactaatggg gtcgggtaaa
     2461 tgccagcggt acaacccatg catcggtaaa gcatcatcgc caaaaccatg aactggattt
     2521 ggattaggtt ttccagcact aaaataatcc cgggtgtgat ctcttttaga ttgaaaagga
     2581 tagcccataa atagcgtatt aaaattttga gcatatgccc tttctccaaa gttcttttta
     2641 tggaaagccc aagcgccgcc aaaataatct tctgctcctg tagatgttat tgtagggaaa
     2701 tcttcatctc catctatata aaacttaaat tctccttccc cccaccagta tctttccaat
     2761 gcgcacaaag caaagtaggt tcctacataa tacccagtgc ctttgattcc atcaataagg
     2821 gtgtaatctt ttccttttgt cgtgatgggt tcacggttcc atttagcatg aaaatacatc
     2881 gcatcatccg gtacttcagt caatgcataa ttaaatgtat aaaaaatgtg cttaataaac
     2941 tcaggatgtt cattcgttaa agtaatctta gctttttttc gaaatggcat ctcaaaatag
     3001 caattcatcc ctgatgttgg attcactacg attggtaagg aattcacttc gcatcttgtc
     3061 ccaaacccat tacaaaaaaa atctcctaat ggactttcta cagaaggagt ggattcatca
     3121 tcccaataaa tacgcaaaat taaatcacgt aagacgaaac tcccagcgat tgttttgtct
     3181 gtcactgtca tccaaaaatg acgtaaaatt ccagggcctt ccatttctgc taaagtaatt
     3241 gtctctccag catttatcgg aacagaaggt gacccttttc tcccaattcc tagatgactc
     3301 gctgtcattg ccgctttccc tttttcacca tttttatttt ctggtgaaat agttctactt
     3361 tcctctccct tgtggagcca tatattattt aaacaattca ttttattcgt cctctatcct
     3421 ttcttcactt ttttgattat tcatatagat gatttgttgt tgtgcctccg caataattgc
     3481 tccaatatct aaattatcat ttcctaactg caaagcgatt gacattgcca agggaagatt
     3541 cattcctgca attacgtgca ctctgtcaga aatataggta aaaaactttt gatttacact
     3601 tcctccagcc atgtcagtta aaacaattat ttcgtctgta gtatccacta actctttaat
     3661 ttgttcatct atatcatcaa tttcttcgac atatgcacat aagctactaa tttgattatg
     3721 catacctgtt aaaaactcta gtgtctcttt aaaaccctca gctagtattg aatggctagc
     3781 aatgatcaat cttctcataa ttcaaatcct tttcctttac gctaatattc caaatgcagc
     3841 cataaccatc gcaaaaacaa taacgagtag aatcaaaatg gtcatactga tttttttctt
     3901 actgagtaga taatatgctg cagcagtaat aattactgga attaatgttg gtaaaatttt
     3961 gtccaacatt ggttggattt tcatactaac ttcaccaaaa gtgaatttaa gtggcgttga
     4021 aacactgata actgaagcaa ttaatgatcc aactactgtt agccccaata ctgaagcagc
     4081 ttcagttaaa taattaatct tttcaccgta ttcagtaaca atcttcattc cctgcttata
     4141 accaaattca aagcctctca ttcggaaaaa gaagattacc atgattaaaa tgaaccataa
     4201 aaagattcct acaggattat tttctaaagc catataagcc gcaattgatc ccatgatggt
     4261 tgggatcatc gtcatcaaaa gagagtcccc aataccagca attggaccca tcgtaccaac
     4321 tttaaagtca ttaatcgcat cttttgcttc taatccttgc ttttcctcca ttgcaacaca
     4381 agcacctaaa ataatggctg ccagccaagg ctgtgtattg taatactgaa agtgattatt
     4441 taaagcttcg atgtattcat catcatcttt gtataatttt cttaaaatag gtgacaatgc
     4501 gtaagtcaca gatgcacctt gctgtgtttg ataattaaag gtacaaactg acatcaacca
     4561 acgatagttc gctttcctaa gatccttttt cgttacttga tatttttcgc tcattttatt
     4621 catcctcccc aataaaccca acatcagaag ctcccgcaga agcagtgatt tgtgcatctt
     4681 tttcagctct atgatacaca ttaaatgcta aagcgaatcc tatgatcgcc gctccaataa
     4741 taggtacctt caaaaaagcg gctaagaaaa atccaacaag taaataacta atgtatcgac
     4801 ctgtaggcat aaagcgtaac aacatcgcaa taccaacagc aggcaacatt tttccggcta
     4861 atgttagccc atccataaac caattaggta aaacatccaa aacgtaatta acagcagact
     4921 gtcctaaagt aacggcaata aatactggaa ttgctgcaga taaaccaaag aaaattggcc
     4981 ccaaccacaa aacactattc attttcttaa attctcgttt attcgcatat tcctgtgatt
     5041 tgcgaacaac aaaaccattt aagattttga caattacatc tagttgcacc cctaacatac
     5101 ctacaggtag tcctattgct aatccggctt ctactccaag ctggctacca aatgccgttg
     5161 caataattgc gcacgttcca tagtccggta cagatgagcc accaaaaccg gcaactccta
     5221 atgacatcaa ttgaactgta ccgccaacta tcaaacctgt ttttatatca cccatcaaaa
     5281 ttcctgtgaa taatccccaa aaaactgcgt aatttacaaa gatcatcgga atcccataat
     5341 aatcaacatg tttaataaat gctaatatag tcagcataat aatttgaaaa atagtaaaat
     5401 gcattatcct tactccttta tcctaatttt gattgaattt cctgtgcctt atccgccgga
     5461 acaaattgtg taacaatttc tacaccagaa tccaacaatt ttttatacgt gctgatattt
     5521 tcatctgatg cataggctct agaagatagc tgtaatgcat tatctttaac aacagtaccg
     5581 ccaacaatta gtcggtcaat tctaataccg ttatctacta aatgaaggat tgtttccggc
     5641 tctttcacga tgacaaatac tttttcctga tcgtatttcc cctttttaaa attcgcaatc
     5701 gctgtttctt cagtgataat tgatacagcc attccagcag gtctagctaa acgcatactt
     5761 gatttttgga tgtcgttatt tgcgacagaa tcatcgatca ccatcactct gtttgcttta
     5821 gacattggtg cccattgcgt gacaataatc ccatgcaata aacggttatc aactctcgac
     5881 ataacaatac tcataaatac ttcctccttt atctttcatt ttcttacaag caattaccat
     5941 gccaaaacaa aaagaacttg ataaatcaag ctctttttac ataatcataa acgtatttaa
     6001 cctcatgaat cgggatgtcg acactataat aatgttcaac gacactgaaa gattgcttaa
     6061 aatgtgtctg aaatttttga aattcctcac tcgtttcatt gaaataaatt tcatcttcca
     6121 aagtattttg ttttactaat cgttcaatta agcaactaat gtgaacatat aatccgaaac
     6181 acgtattatt atcaaatgaa attcctagat cattttgtaa gatgtatata gcatctgaaa
     6241 cccgttctaa taatttagtc ggatttaata tggtcaattg ctccattaaa ttcgttagac
     6301 taaaattctt cagaatatta ttgttcaaaa ctttcagttc attatcatta aagtgtctat
     6361 tcaattgatt ggagaattta cttatattca ttcctaaaat catatcttca attgaaaaga
     6421 attctacatc ttctagatgt ggatttagtg ttccaacaat aaaaagaata ttgttctctt
     6481 tacgaagctc cgataaatag ttttcattca ctaaagagta atagtcacac gtttctatga
     6541 caagatcagt atcccttgga aagctatctt caagtatctt ttttaattta aacgaagttc
     6601 ctaatccgct tgcacaagaa cagacaatca tatccttctt aatatttgat gattcataaa
     6661 tataatccac attttgattt tcttttactt gttcaagaat ctgtttaagc ggtaaatcta
     6721 ataatatctg atttgcaaca tcaatagcca ttcttgttga aacattgttt agtagtccga
     6781 attttttttg agcgtctagt tccaaattta gataaatttt ctccagtgaa cccatatcaa
     6841 caagtaaaat aatttcttca tatccggtac actcttgtaa gtatctatta attttgtctg
     6901 caatctcact acttgaagtc attagtggca tatcaatggc gtcgtatata tatctctcaa
     6961 cgatcctatt agcagtactt gctatacagg ttgccgttag atctccatga gccaaaatca
     7021 gtgctaaacg tttttgctga aattctttac tacacattaa cttaaataaa ataaccaaaa
     7081 catttttttg aatttttgtt aaataaatat cccctatttg aataaaacga ttgactaatt
     7141 gaacttctcg actacaccaa ctattaatat cattgatgaa ggaatataat ttatcgtccg
     7201 atgttaattg gatttgataa atccaagaat tcaaaaagat agacgttatt tgaagataat
     7261 aatagtcaaa ttctatttga aaatctctct ctatttcctt aacatactgt ctaaatcgaa
     7321 tctcttgata ggtgggatat gttaaagctg aattattttg gatatcaaca ttcttttgtc
     7381 taaaatatct atcatatttc tcaaaaagct gctgcttatc agtgtctttc ttccacgttt
     7441 gataaagata ctctataagt tcgttgatat taaaaacttc gtctactttt tccaaatcat
     7501 ccatttgaat atattttaat tcattctgtc ttttcataac cttcatgccg ttgaaattca
     7561 cccattgtgg taaatgatac atctgaatat aaagttcttc caaatcatct aagaaataaa
     7621 aggcattggc acaggtcaat tgaatgatat ttttcaaatt gccaacattt tcactaaagc
     7681 gatagtttaa taaaaaatct aataattgtt gagatatcag tattttcttt gctagtcttt
     7741 cttcttctga tttaaaaaat atcttgatta actgttttct ttcataaaga gaacgactgt
     7801 ctaatgttgg aagtgttagc tttacaggta ttcttctcaa caaagttgat aatagtttat
     7861 cctcaggtgg ttctgatgtt gcaaatataa tccagcaact tgatgtgtac cagttttcat
     7921 tatcacccat ccgatgaaat ttcctcgtat ccataaactg aaaaagtttc tcctgacatt
     7981 cattgctcaa tgcatgaatt tcatctaaaa ataagactcc attattagct aattctgcta
     8041 aaccctttct tccatcttcg gctcctgtat atgcaccttt tacatgccca aaaaaattgg
     8101 acaagaaaag ttctggatta ttggcatatt cactacaatt aactgaaatg aaagatgcct
     8161 tctcttctat ataattattg gccaacaaat ccttatacac ataatttgct aaatagcttt
     8221 ttccagttcc agtctcgcca tgaagtaaaa tgggtaagcc atttggcgga tagtagattg
     8281 ctgctttaat ttgagcaatt acatttcgca gacttccttg acttccgata accctttcta
     8341 ttccaaccct aaaagagcta tcataccatt tttccgcttc ctctgaagag ctaaaaattt
     8401 gttgatttgg aactttctcc ggtaaagaaa agaagattac tggacgagta ttgatttttc
     8461 caaattctcc atcttttacg aattcattca aatattgaga gacaatatta cgactaacat
     8521 ttaatttttc agatattttc tgtgctgtat tgttccccca tttattttta atctcagagt
     8581 tctctatgac ataattgaca atgcgttctt ttaattcttc catcacagca cctcctcgac
     8641 actattcgaa ttttgccgac agtatttttc cataaatatg acttgctaaa aaggctttgt
     8701 ttagatcatt caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat
     8761 actctggcga aatcgcatac cccaaaatat caacgattgt tgcaatcaga tcttcttttg
     8821 attcttcatc tgaaagtaat tcacgataca acatcgtttg tttgcttact gtgtcatatt
     8881 cctctaagga ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt
     8941 tatagaaaat cagccccaat aaataatttt tatattctga cgcatccatt ttactacgga
     9001 gattatctgc cgcactaaat aacttctgat ttaattctgc acccaaaaag atacactcct
     9061 atctcttatt taaagttgct atataccctc ttattttagc atactcagtt ttagaaaaag
     9121 caaaaaaata aagaaactta ttcttgataa caagctacgt gacgtatatc cctaacacaa
     9181 aaatagccat atcctttggt ttggatacgg ctatttgata ttactcattc aattatcaaa
     9241 tcacagtagg tgtgaatctc atcgggatcg gcaatatcta cttctgatta ggatcaaagg
     9301 tgattttctc aataaaggtg tgatacaaag ctttaacttg cttcttctca ctctggataa
     9361 gaaagcaatc caaactagtc aatatctgtt ggacatcttt gacttgaatc ggttcccctt
     9421 gttgttgaaa gatccctaag atttcattct ctcgaatttg taactgtctt cgtttttcag
     9481 tcaattcatt taaacgatct tttaacatcg gaaacaattc gggactatct tccaaagcag
     9541 cttcctgttt ttgctctaat agttagattt tgcgtaccat tttcttcttt tattttcctt
     9601 cgattgctga ttttaatata gtctaaagga aatttatatt tattgtattc tttataactt
     9661 caagagaact tcgtttgagt attactgtat ttttttaatt ccataagaaa attagttatt
     9721 acctgccacc taatcagcaa atctgaacct gagtcaatat aattaatatt attttccatc
     9781 tccaacattg taagtactgt tggtgttaat atagggactt tgtacaattg tatttttttg
     9841 attttatcat ctgcacaaag gagatttttt aaatctaatc cttcttttat ttcaaaagtt
     9901 gaatttttta aatctataag agtaaaacca attccttcat actgaatttc tgaaaatcga
     9961 atttttctcc cagttgatag atcaaggggc agaaaacctt ttattacatt gtgagtgtca
    10021 ccatcattta caatgtcatt aggaattttg attttagaat tctgtggagc acctattgga
    10081 agataataat tcattataat tttgtagtct tcatgaggaa ataaagtact tttaggattg
    10141 ctaagatatt ctcgtaccga atctaagaat ggcttatcca ttgaatcttc atccccattt
    10201 aaactaaaaa acattcctac aattgctttt gcaactctaa gtgaattcat tttctttatt
    10261 gaatgtgtta aatcagactt accattcttt aaacttgtat tgttataaag gaaattttta
    10321 ttgtcttctg caaatctaat atattcctca acatactcac taccaaaaaa actattacat
    10381 ttactacata acgtattgga atggcaacag tttttttgac aaattttata aggtgcagaa
    10441 cttctttccg tatgctattc cgattgtcct tgacaatgag cctcctcgga tgtgacgatc
    10501 ttcggcagga gctaacagtt aaagttagac tgcctcgcta gcgttagcac atccgagctc
    10561 attatcaagg attcgctgcg ccaat
//
LOCUS       DACDRK010000085        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDRK010000085 DACDRK010000000
VERSION     DACDRK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.085"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IWJ77_002368"
     CDS             complement(247..759)
                     /locus_tag="IWJ77_002368"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9453925.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWJ77_002369"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWJ77_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9453926.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IWJ77_002370"
     CDS             complement(3212..3646)
                     /locus_tag="IWJ77_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9453927.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IWJ77_002371"
     CDS             complement(3656..4444)
                     /locus_tag="IWJ77_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9453928.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IWJ77_002372"
     CDS             complement(4441..5382)
                     /locus_tag="IWJ77_002372"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9453929.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IWJ77_002373"
     CDS             complement(5559..6146)
                     /locus_tag="IWJ77_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9453930.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWJ77_002374"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWJ77_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9453931.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IWJ77_002375"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IWJ77_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9453932.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IWJ77_002376"
     CDS             complement(9063..9998)
                     /locus_tag="IWJ77_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9453933.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACDRK010000086         9386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDRK010000086 DACDRK010000000
VERSION     DACDRK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.086"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(267..881)
                     /locus_tag="IWJ77_002377"
     CDS             complement(267..881)
                     /locus_tag="IWJ77_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9453934.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1487..1864)
                     /locus_tag="IWJ77_002378"
     CDS             complement(1487..1864)
                     /locus_tag="IWJ77_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453935.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2164..3045)
                     /gene="galU"
                     /locus_tag="IWJ77_002379"
     CDS             complement(2164..3045)
                     /gene="galU"
                     /locus_tag="IWJ77_002379"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9453936.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3088..4107)
                     /locus_tag="IWJ77_002380"
     CDS             complement(3088..4107)
                     /locus_tag="IWJ77_002380"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9453937.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4740..5744)
                     /locus_tag="IWJ77_002381"
     CDS             complement(4740..5744)
                     /locus_tag="IWJ77_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9453938.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5757..6806)
                     /locus_tag="IWJ77_002382"
     CDS             complement(5757..6806)
                     /locus_tag="IWJ77_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9453939.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6823..7644)
                     /locus_tag="IWJ77_002383"
     CDS             complement(6823..7644)
                     /locus_tag="IWJ77_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9453940.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7631..8410)
                     /locus_tag="IWJ77_002384"
     CDS             complement(7631..8410)
                     /locus_tag="IWJ77_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9453941.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8427..8897)
                     /locus_tag="IWJ77_002385"
     CDS             complement(8427..8897)
                     /locus_tag="IWJ77_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9453942.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8909..9328)
                     /locus_tag="IWJ77_002386"
     CDS             complement(8909..9328)
                     /locus_tag="IWJ77_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9453943.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
BASE COUNT         2755 a         2029 c         1495 g         3107 t
ORIGIN      
        1 tctttcttgt ctctttttcc actacttttt atttttctga cactacatat tcttataact
       61 catttctcat aactgtgaaa caacttataa aaataacaaa ctttaaacaa aacaagctta
      121 aaatcaaaga tgatccatct taaaataact aaaacaaaaa aagtagtcgt tgttaatcct
      181 cattaataat cgttttcttt ttttcaaacg ctccattttt atgtggaaca ttattttttt
      241 aaggaacttt gtcaacatgt ggataactat tttccacttg tgtcctggac tagctgtttt
      301 gctgtaaaat acaaaacttg ctgcagcaaa aagatcgttg caagagaggc tgttccgcag
      361 atcgctggat ctagatttgg caaagtcaag actgcatcat gtaaattggc gtggttgtcc
      421 aaaaccaaat cagcaaattc aaataatcgc aaaccactgc tatgttgcga tttgatactt
      481 cgtgacaaat caaatccagt aacaatgatt aatggatgac cattttcttt gatccactga
      541 gctagttcga tgattgccgg gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct
      601 gatttgacag aaagctgatc aataattgcc tgtcctgcac cttctagcgt ttcatagaca
      661 cctccggctg gattttcaat caatatactt ggtacaagat ccggggtttg atcccaaaat
      721 tcaaatgcta caccacttag tgtgcggcta gcatatatct gcaatctacc accttgatta
      781 atttgtttcg cgatccaagt gccagcttga agcatcagcg gctcttctat ccttaatttc
      841 tcttccaatt ggtgataata gtcaaaataa cataaactca tttatctctc ccgcctagct
      901 atacttgaca tgaatagaaa cagctgccta atctaaggaa gtacataaaa aaacagtagt
      961 tagacagtaa cacatgtggt ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac
     1021 gcccaatacc ttgacatagc aaataaaaca acgtgcagac catggaaaat atctacatag
     1081 gggtagaatt ttcaccctag attaggtaca ataaattagg tcgatgcact gcattgacta
     1141 tggcaaaatg atcggtcagg gtgtatgtgt gtgactggtc attccctgat tcttagcgcc
     1201 aactaagggt cagggagcct ttttttattc agctgtttct atctgaattt attgtaagta
     1261 atctcttatc caagtgcaag caagaaaata caaaaaaaca ttggtatgat ttatatatat
     1321 ataaataaca taaaaaaagg aatggcttat cccgatacaa caagcattcc tttaaaaaag
     1381 attcttttat ataagtgaaa aaagcttttt tccaccaatt aaataaatct ctcatctcaa
     1441 tagtttatcc attattttat ctttttttgc gtatacctac taaatattac cgtcttcttc
     1501 tcgttcttct actttgttcg agagaatctg tactagaaaa agtagttgta tgtctacctt
     1561 ttctgcctat ccgagacttt cttttcatat atgggatata aatgattaga tacagtacag
     1621 caaaaaataa agcaaatacg caagcttccc aactacctaa cgccaattca accatcaagg
     1681 tagcaacccc aacggatgct gcataccaga taaaaatcag aaacgtttct ttttttctga
     1741 ccatgcgaaa catgataaat agactaataa gcaatagtgg caacacaaat atccaattgc
     1801 ttccttccat cagccacttg agtatatcaa gcagcgactg tgcaacagcg actaattttc
     1861 ccatagtgaa cacacacctt tttttataat taaaaaattc gcctttttat tctactatga
     1921 atcttctggc ttggctttca tttctcttat gtaatcattt tcttttatac aacttaaaaa
     1981 aaaattaaac cattagcata atattttctg aatctattca cgaaagtctt tttctatctt
     2041 tcgttttcca cgactcaact gatctagagt caaaaaacta aaacgacagt tgcgcaaatg
     2101 acgcacaagc tgccgcttta ataacaaata gcctttcatt ctcagcaggc cacttatttc
     2161 cagttaactt tctgttctta gctttttact caactggata atatatgcac ttaatgaagt
     2221 cccaatttcc gaatgttgaa gtccatattc aatatttgtc ttcatatatc ctaacttatc
     2281 gcctacgtcg tatctttttc ctttaaactc tctcgcatag accgcctctg tctggttaag
     2341 agtctgaagt gcatccgtta gctgtatctc gtttcctgct cctggttctt gagtttctaa
     2401 tatctcaaat attttaggag tcagtagata acgtccgata atggctaaat tacttggtgc
     2461 ttttcccatt tcaggttttt ctacaaaccc ttttactctg caacgaccct tttcgctatc
     2521 tgttttagga tcaataattc cgtatttaga tgtttcattt tctggtacac gcatgacggc
     2581 aacagcggaa gcttgtgttt cttcataatc catcatcaat tgttgagtca atgggatatc
     2641 atctttcatc aaatcatccc ccagcatgac gacaaacggt tcatttccta taaatgcttt
     2701 tgcttgaagc acggcatccc ccagtcctct tggatgagat tgacggatga agtgaagatt
     2761 gacttgagtc gtttcttcaa ctaaagcgag taattctgtt ttttcctttt cccgcaaatt
     2821 gttttccaat tcgatatttg catcaaagtg atcttcaatg ggtctttttt cttttcccgt
     2881 tacgatcaag atgtcttcga ttcccgaatc caatgcttct tccacaataa actggatcgt
     2941 tggtttatcc acgattggaa gcatttcttt tgccatggct tttgttgccg gcaaaaaacg
     3001 tgttcctagc ccagcggcag gaatgactgc ttttttgacc ttcacttgct gttcctccta
     3061 aacgacgatt aactattgat catctattta acgaagagaa aattcattct ccatttttcc
     3121 gtcacgtaac atgatttctt ttgctgcttt tttgatatct tctccattat acaaaacatt
     3181 atagattgtt tgagtaattg gcatctcaac acctagctgt tgagctagct ctactgctgc
     3241 ttttgtcgtt gccacaccct caacgaccat tcccatgttt tctagaactt cttccagctt
     3301 atgtccctgt cctaacaagt taccggcacg ccaattgcga gaatgaacac ttgtacaagt
     3361 aacgatcaag tcccccacac cactcaaacc gataaatgtc agaggattgg cacccatcgc
     3421 gacaccaaga cggctgattt ctgccaatcc tcgagtcatg attgctgctt ttgcatcatc
     3481 tccgaatcct aatccatgga tagcgccggc accaattgca atgatatttt tcagtgctgc
     3541 accagtttct acgccaatca catcattatt tgtataaatc ctgaaataat cattcatgaa
     3601 cagtttttgt acataggctg ctgctttttg atcaatactt gcagctgtaa ttgttgtgat
     3661 atcatgtact gccacttcct cagcatggct aggaccagat aatacaacaa ttgcctgtct
     3721 tttttcttct ggaatttctt cagccaaaac ttctgagatt cgtttatgcg tatcttgttc
     3781 tagtccttta cttgcatgta taatgattgg cttagtattc attttaccaa taagttcctg
     3841 tgcgactgaa cgaatcgctt ttgttggaac aacaaataaa atagcatctg cgttatccac
     3901 tgcttctgcc aattctttat atccgataat cgattctggg atcgtcaaat caggtaaaaa
     3961 atgctgattt gtatgatact tattgatttc atcaatctgt tcaggtacat ttccccaaat
     4021 acggacatca tgcccgtttt ctgccaatgt ttgtgctaaa gcagtgcccc aagatccagg
     4081 accaagaact gcgatttttt gtttcatgga tattcctcca aataaataat tgataatttc
     4141 cattatagta tacatttctt aaagttggga ctaatttcca ttgtttttaa gtgtttgttt
     4201 acttctttta tcgtttttac ttgaaataat caatgatatg ttacaatagt ctacatttta
     4261 tttttacact cgttatgctt tgcttgttcc taagaggaag cagttttgac aatgttacta
     4321 ccagcattac tattaggagg ttaggaaact tctcctagtg agtattgatt taaaatacat
     4381 aagagacaga ttgaacattt ctaaatctgt caggtcctgc aacggacaga ttgtctctgt
     4441 tgacggttga cataggaact tttgttttag tcaacattct ggatacaaaa cgctatgtaa
     4501 actggcgcaa ctcgttgtac ttggactttt actacggatc catgcaacaa ctaaatagta
     4561 ctattgcata acaaaaaatg gttggcaccc acatgtccca ttaaacagat tataagttga
     4621 actgtagata atgcaaaaaa tgtgttttat aacgtaattg atgccaatat tttaaaaaaa
     4681 taaagggagc agttatcgct gctctcttta ttttttatat tctttttatc cagtctatcc
     4741 taataatcaa actgacgata gtagcggcgt atctctaatg gatggcagtt caactgctcc
     4801 acatgagcat cgatgcgttg tgtcactaca tgcatcaata agaaagacaa aacacgtccg
     4861 cgatattttt cactgatacc ttctactgga tagtcttttg tgtctatcaa tgtataattg
     4921 ctgcaaattt ttggcaaaag tttagccaca cgttcagata acactctctg ttcatcttcg
     4981 cctaaaaata atgtgactgg ggttgtttct tctacgatct ctaacgtccc gtgaagaaac
     5041 tcggcagcat ggatagattt cgatggaagc cagctttgtt cttcccagta acacatggca
     5101 tatgaataaa cagctcccca ttgattccct gctccaataa aataatgcat actatcacgt
     5161 cgatgttttt ctgcaaatga taagccaaat gcatctgctt ttttctctgc ttccactaac
     5221 cctgttggta aataacgttc gagctgttca tagtattctg aataagcttc gaattctcca
     5281 ttcaaataca tcaatcgatc tgctgccata aaaaatttga tttgttctgt tccaggagct
     5341 ggataagtga ccacaaaatc gcatagctga gacaacttac tgttcgcttt atcgataaat
     5401 ccaatcagtg tcgctcctac tttcttgatt tgttctactg ctttcactac ttcttgagtt
     5461 gtaccggtaa cagaggacaa aataacgata gaatcttttg ttaatctttt gtttccagta
     5521 gtgtaatatt ctgcagcatg ctgtacaaag acaggcaagt tgcttttccc attcatataa
     5581 gtgacagctt gcatcgagga agcatacgtt ccaccgattc ctagataata gacagcgtca
     5641 aatttttccg cataaagttt atcgatgatt tcttctactt gcggacgtaa agctagtgcg
     5701 ccttttattg ctttgatttg ttcttcttca ttgaatttca gcatgatttc cacctcttat
     5761 aatttacttt tcaagactgt atcaaagtct tcaaaaatct ttttgttcaa gtcaatacct
     5821 ttagctactg cggttttata agcgaatagt tgaaacggaa cgacaagcgc taatacagac
     5881 agatattcat cacattctat tgctaatcca agtgttttct cgtttcttgc tttttttgtg
     5941 gtaatggtga gagtctctcc aacgtaccga ctcatatatc tttgcaactt ttgcgaacgt
     6001 tccttattta cactttcccc ttcaataaag aatagcaaat gcgaagcatc tgcctccagg
     6061 tatgggccgt gcatataagc ttctaactca aatccttgtg acggaacgcg gattgtttca
     6121 gtcaatttcg tctctgcttc tttagctgtt ccccagtttg gaccatatcc aatggtgacg
     6181 aaacgctgtg ctaaacgaaa taaactttga tatccatcaa aaaattcttc cgttttttga
     6241 ataatcgctg gtaaatggtt gattattttg cgcaactgtt gcatgtagtc ttgttctatt
     6301 tttttgctga ttttattttt acttattcca attcctatcc ctaagagtag taactgtaaa
     6361 acagtcgcag aatagccttt tgtaacgaag cctactgatt ctaccccggc acctaaatca
     6421 ataatttgtg cagcttctaa agcaagtgga ctttgcacgt catttgtgat agcaacaaca
     6481 ggtaaccctt gtttttgtat catttttact gcttcaacag tagaagcact tttcccgctt
     6541 tgtgacacag caatcacagt atcgattgaa ggagaaagtt ttccataatt gaaaaaataa
     6601 aaaggttctt gaatatcgac agtaagatca ccataagatt ccaatgctgg cttcgctgct
     6661 aaacaagcgt tataagatga acccgttgct aagatcagta aattcactgt ttttttcata
     6721 tgttctcttg tcgaaaaatc attctttttc aagatcgtac ttagtacttc cttttcttca
     6781 tagatataat cttgcatcgt aaacattttc tctgctcctt ttttaaaaaa tccctatcca
     6841 cgaaccaaaa attccaaaga aagctagtcc gatcaagatc gttaatggtt tcacttcttt
     6901 cttcaataga taaaacacta tgccaaatgc tccaagacat aagatatttg gaacgatatc
     6961 atcaaaaata ctttgaacag tcacagcatt ttctcctgaa ccgattttta aaggaatatt
     7021 gatggtgatc atcgtggcag tcattgctcc aacgaccatc agtccaataa tagacgcgcc
     7081 taatgtcaaa ctttccatca taccgtttgc ctggatcttt ttcaaaaaac ccgttcctaa
     7141 gcgatatccc caacctgttg ctagatagcg aaacagaatg tgcggaatat tgaaaatcaa
     7201 aatgaacaag atagggccta aaatatttcc ttgcatggct aaagaggtac ccacacctgt
     7261 ggcaatcaat cgcaaagttc cccaaaagaa agaatctcct aaccctgcaa gaggtcccat
     7321 caaagatgtt ttgatactat cgatcgcagt cacatcaaat tcaggatcat ccttattttc
     7381 ttcctccatt gcagccgtga ttcctaaaat cagtgtaacg atatgtgggg tcgtgttaaa
     7441 aaactctaga tgccgtttca aagctgctgc catttcttct ttcgactgat ataacttttt
     7501 caaaatcgga atcattgcat aaacataagc catattcatc tgacgttcat aattccaaga
     7561 aaactccatt tgaaaagaac gccaaaatac gtgatttagc tcttttttcg ttattttgct
     7621 gtttttgatc tcagaattct tcatcttctt ctacctcccc tgcagttgtt tgaagtggta
     7681 gttgacggac attcataata ttgactacca caactgctaa aatcgcacca aaaatagcta
     7741 ctccggtcac aggaatctct aagtaagaag ctatagcaaa acctaagaaa aaatatggtg
     7801 caacttgttt gttgatcagc aaacgagcaa gcatcgcaaa accaagagct ggaatcaacc
     7861 ctgtagctac tgacaacccc ttttgaacga agtcaggaat catatttaat agatttccga
     7921 tcgtattgct tcccaccata taagaaacca ttaccacaac accaagcatc aaggaaagac
     7981 cgataccggc aagcaagtgc atatgctcga ctcctttata atttccttct tctgcataat
     8041 tgtctgcttt atggttcata attggaatca aaagccctaa gtaaatattt ttcaatatca
     8101 atgtcaacgt agcaatcggc aaacctagta aaagcgcggt ttctgtaccg gctccagctg
     8161 taatagcaaa agccgtccct aaaatcccac cggtcacaac atcaggtgga atcgaagcac
     8221 ctactgagaa cgatccgata aatgctaact ctaaagttgc gcccataatg atcccagttt
     8281 ttacatcacc taacacgatt cctgtaaaca gccccgtcac aatcggacga gaaagcaacg
     8341 atgtccctag tgcatattct gattgtgcga caaatgcaac aattcctaat aagactgctt
     8401 gtacaatcat tgaggttctt cctttcttac aaaacttctc ttacatccac ttttttatct
     8461 gctggtactt gacggatttc tacttctatt tgtttgtcaa ttaatcgttt caagcgttcc
     8521 tcctcttctg gtaaaatatt taccgctttt ccgatgctgc gagtaccagg ttttgccttg
     8581 atccctccta agttgatggt tttgatttct ggatacgctt ctgccagctt atatgcatcc
     8641 tcgatcgatt ccactacgat gaataactta tatttatctg tcaccccgct tttcaacgcg
     8701 gcaatcgagt catcaattga tttgatcact agtttgactc cttgtggctt ggctaatttg
     8761 atcgttgtct ttcgtatctc attcgtaggt acatcatcat tagcaatcaa aatacagtct
     8821 gcgcctattg tttgtgtcca tgaaaaagct acttgtccgt gtaataaccg atggtcgact
     8881 cttgtcaata caatcatttt tcttcctcct aaaattcgtc ttcttctatt ttctgattga
     8941 gccgctcgtt acaaaactgg atcatttttt ttgaatcagt tagcacctgc tggatcaaaa
     9001 cagttgtctc tacttcagcg tccaaccgag atactagctc aatcaatagt ggcaaattca
     9061 atccggcaac gagatagaca tgatcccgat tgatatattg taagaattcg ttgttgacac
     9121 ttccgccaaa aagatccgtc agaacaatga tttctttatc tttgtacctc ataaaaagct
     9181 gccttactgt ttccgctaaa tcaaaattct catcagtata gcaatcgaat acagtaatag
     9241 aatgattttt cccatagatc agctctaatg aatctaatat ccctgaagca aattttccat
     9301 gggaagctaa gataatttct ctatccactt tttcatctcc tcttttcact tacacatact
     9361 aaaaaagcaa gttgcatgcc aaaatt
//
LOCUS       DACDRK010000087         9360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDRK010000087 DACDRK010000000
VERSION     DACDRK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.087"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(91..759)
                     /locus_tag="IWJ77_002387"
     CDS             complement(91..759)
                     /locus_tag="IWJ77_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9453944.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            893..1180
                     /locus_tag="IWJ77_002388"
     CDS             893..1180
                     /locus_tag="IWJ77_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453945.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1596..3134
                     /locus_tag="IWJ77_002389"
     CDS             1596..3134
                     /locus_tag="IWJ77_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9453946.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3131..4096
                     /locus_tag="IWJ77_002390"
     CDS             3131..4096
                     /locus_tag="IWJ77_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453947.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4083..5405
                     /locus_tag="IWJ77_002391"
     CDS             4083..5405
                     /locus_tag="IWJ77_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9453948.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5468..5572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5646..6389
                     /locus_tag="IWJ77_002392"
     CDS             5646..6389
                     /locus_tag="IWJ77_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9453949.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6404..7396
                     /locus_tag="IWJ77_002393"
     CDS             6404..7396
                     /locus_tag="IWJ77_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9453950.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7408..8145
                     /locus_tag="IWJ77_002394"
     CDS             7408..8145
                     /locus_tag="IWJ77_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9453951.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8135..8857
                     /locus_tag="IWJ77_002395"
     CDS             8135..8857
                     /locus_tag="IWJ77_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9453952.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8941..>9360)
                     /locus_tag="IWJ77_002396"
     CDS             complement(8941..>9360)
                     /locus_tag="IWJ77_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9453953.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2713 t
ORIGIN      
        1 ttttttataa agaagatttt actgaataat atcattttag tttacataat gatatttctg
       61 taaattaaat aaagtagtga aaaataagaa tcagtgctct aactgttcaa gaggatcaag
      121 tggttcaaat gtttctgtag tctgttttgc ttccaattgg ggcaaaacag atccaatttc
      181 cagatacttc actttattca atgtataaga tagaatagaa cagctaagta atgtatacag
      241 aaaatgtgtg agatccaaat aaagcaagga gacaactaaa ataccaaaag taatcaagaa
      301 aagacttttg ccaatcttaa tatgaaaata tttttcaatg gcttgataaa tgatatctgt
      361 tccgcctgtg cttccgccga atcgaaagac gagaccgcaa ccaatgccag aaagaatacc
      421 tgtcatcaaa ctattgatca gtagactatc tacagcaaaa tgtatatgaa ggctctccca
      481 taaatgtaaa aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt
      541 tccaaaaaga aacaatgaga taagcaataa aggtaaatta aaaagcagtg aagtgacaga
      601 aggagcgaat ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc caccatctgc
      661 cagcttagat ggaatattga ttcctgtcac agctaatccg tagattcctg cgcctaaaat
      721 gattccgcac aattctgtaa ataaatttaa ttttttcatt tctagcctct tttcttttga
      781 aaattttcag caacttcttt attatactga cttatactct tacttcaaga ggaaaatgaa
      841 aaaaaattat ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc
      901 agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt
      961 tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca
     1021 atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt
     1081 tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct
     1141 ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga
     1201 cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag
     1261 tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt
     1321 ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa
     1381 gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt
     1441 gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt
     1501 ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat
     1561 gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa
     1621 gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt
     1681 ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa
     1741 aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct
     1801 tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg
     1861 ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc
     1921 cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag
     1981 tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa
     2041 tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt
     2101 tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct
     2161 tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt
     2221 ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg
     2281 ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc
     2341 tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac
     2401 cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct
     2461 atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa
     2521 gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat
     2581 tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga
     2641 gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc
     2701 agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc
     2761 aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc
     2821 aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat agccagctca
     2881 aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg
     2941 caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata
     3001 aattaagcgg aaaaataacg gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg
     3061 aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg
     3121 aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa
     3181 gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg
     3241 tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc
     3301 agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg
     3361 gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca
     3421 aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt
     3481 agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag
     3541 agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc
     3601 agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt
     3661 acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca
     3721 caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat
     3781 tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca
     3841 tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg
     3901 atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat
     3961 gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag
     4021 aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg
     4081 aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt
     4141 atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga
     4201 cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta
     4261 atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag
     4321 aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat
     4381 tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta
     4441 ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac
     4501 ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat
     4561 tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag
     4621 ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat
     4681 taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca
     4741 ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt
     4801 tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc
     4861 cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg
     4921 cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt
     4981 caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc
     5041 ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg
     5101 ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag
     5161 tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta
     5221 aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc
     5281 aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag
     5341 agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga
     5401 aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa
     5461 aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga
     5521 cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg
     5581 gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta
     5641 acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc
     5701 agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat
     5761 tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg
     5821 aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg
     5881 attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc
     5941 caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag
     6001 tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga
     6061 ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga
     6121 tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg
     6181 ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct
     6241 tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga
     6301 tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag
     6361 ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct
     6421 tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa
     6481 acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa
     6541 tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt
     6601 ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc
     6661 acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat
     6721 accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc
     6781 agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag
     6841 tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa
     6901 actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat
     6961 ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa
     7021 ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac
     7081 gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa
     7141 aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata
     7201 tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta
     7261 tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc
     7321 ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa
     7381 tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc
     7441 attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca
     7501 tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc
     7561 cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca
     7621 gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac
     7681 gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat
     7741 ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg
     7801 atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt
     7861 gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg
     7921 aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca
     7981 atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag
     8041 attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag
     8101 caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt
     8161 agatgggagt cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa
     8221 ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc
     8281 cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc
     8341 ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt
     8401 accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga
     8461 gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc
     8521 ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac aagactatat
     8581 tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca
     8641 actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc
     8701 agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca
     8761 gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta
     8821 tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt
     8881 gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc
     8941 ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga
     9001 agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta
     9061 agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct
     9121 tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt
     9181 ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg
     9241 aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg
     9301 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DACDRK010000088         9066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDRK010000088 DACDRK010000000
VERSION     DACDRK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.088"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(42..302)
                     /locus_tag="IWJ77_002397"
     CDS             complement(42..302)
                     /locus_tag="IWJ77_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453954.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            377..580
                     /locus_tag="IWJ77_002398"
     CDS             377..580
                     /locus_tag="IWJ77_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9453955.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(721..1206)
                     /gene="ssb"
                     /locus_tag="IWJ77_002399"
     CDS             complement(721..1206)
                     /gene="ssb"
                     /locus_tag="IWJ77_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9453956.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1209..2108)
                     /locus_tag="IWJ77_002400"
     CDS             complement(1209..2108)
                     /locus_tag="IWJ77_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9453957.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2121..2714)
                     /locus_tag="IWJ77_002401"
     CDS             complement(2121..2714)
                     /locus_tag="IWJ77_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9453958.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(2955..5159)
                     /gene="topB"
                     /locus_tag="IWJ77_002402"
     CDS             complement(2955..5159)
                     /gene="topB"
                     /locus_tag="IWJ77_002402"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9453959.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(5170..6630)
                     /locus_tag="IWJ77_002403"
     CDS             complement(5170..6630)
                     /locus_tag="IWJ77_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9453960.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(6691..9063)
                     /locus_tag="IWJ77_002404"
     CDS             complement(6691..9063)
                     /locus_tag="IWJ77_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453961.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2217 a         1890 c         1517 g         3442 t
ORIGIN      
        1 aaaaagagct gcaatcctca tttaggactg cagctcttct tttatatgcc catcgatcgc
       61 gtaaaatgat cggggtttct aatttgcatc gattcaatga ctaattgatt ccgtttattg
      121 aaatgacctg tgacggcaat attgtatttt ccatcatcca taattaacaa tttatcgata
      181 atttcaatat ctgcaactac acaattaatc gattcatgaa ttgtttgtaa agtaaagcga
      241 accaataatg gttgtgtttt tattactcgg attttatcaa gcaaaccaac atatttgttc
      301 atcacgatca cctcgctaaa attatagcaa aaacagctaa aattgagaat ttttattcga
      361 ttttagatat gctataatga tcaagacagt aaagtttgtg acggtggcta tccgattgaa
      421 agtgaggtgg tgcgtatgtg cgtacttcca aaatcttaca gaaaggagaa gccttttgcc
      481 tgttgaaacg gcgctaggat taatgattag tttcggttct ttgatcgcaa caatcatttt
      541 tggaatctta gcagttgtaa acaacaatca aaaaaaataa ccgtcagctt tagccggtcg
      601 acgattattt ttcaaaaata atattaagct ggtcaccgtc ttaaacggtg ctgttagggg
      661 agttgttagc gcaactcctc ttttcgtatc cattataaca actttattga tcttttacaa
      721 tcagaatggt aaatcatcat cactaatatc gattgattgt ccgccaaatg gattttgtga
      781 agcgttcaat ccattgttgt taggcgtagc tacatcccgt tgttcaaagg tggtagggct
      841 tggactgctg gacgattgat cagcgtgcgc acggctttct gtcgctgctt tactttctaa
      901 tagttggaaa ttatcggcca caacttctgt cacatacaca cgttgtcctt gttggttatc
      961 gtaggttcgg gtttgaatcc gtccaacaac gccgatcaaa ctgccctttt tagctaatgt
     1021 cgccatcgtt tctgctggtt tgcgccaaat cacacagtta ataaaatcag cttctcgatt
     1081 gccgttttga ttcgtgaaat ttcggttgac agctagggta aaagtcgcta ctgcagtacc
     1141 actcgcggta taacgtaaat ctggatcttt ggttaagcgt cctactaaag ttacattatt
     1201 gatcatcttt atctctccat ttcttgattc atttgttgtg atcttttaaa ctgccgtttt
     1261 ctttcttgat tttcaatttt gatcacactt ttcattaggt gatcaatttc gttgactgta
     1321 tctgtaaatg aaatggaatt cgcaaacgtg ttaactgagc gatatttttc ccactgcttt
     1381 tccaaatagt catttcctct gatcttatcc atttctcttt cataataaaa ggacaacggt
     1441 tttgtatccc cacgttttgt aaatgtattt cgaacacttt tgtataactc tttcgtatcg
     1501 atattttcta gtttcggaat ggtaaataag tcgtagtaat cttttgctct cgaattatct
     1561 gtgccaaagc tgaacgtagc acttagcttt tctgcaataa tttgttctgt tggataagct
     1621 ggaatctcca cttttttatc ttcaaacatt aattggtgat agtgtgtatc tacttcagga
     1681 taaatagaat ctcctgttgt cacgtccatt ttaaacggta cacgtacttt ctctaaatta
     1741 gccaccaatt taatttgaaa tccaggataa aaatcggctt ctcttgtttc cttcattcct
     1801 tgaatagaga aaacaatgcc ttcttttgtg ggttttctag ttaaatcatt aaaaatgctc
     1861 ttcaattttt cttcagttaa tttgctattt ctaaatgtcg tatcgatatc aattgttgtc
     1921 cttctatcga ttccatattt tgatcctaat aaaaagccgc ctttcaaaat gaagtcattt
     1981 ttgtactttg attcagaaat ttgttcaatt agacgatcca tcataaaatg ccgttgcact
     2041 aactgtgcat ttaatcctgt actctttgct aaatttcttg tttttgctgc aaattgtgtc
     2101 ggtgtcatct ttttcctcca tcaatataag ctttgcatgt ggtatttcat ttctttttct
     2161 acatgtagtt ttgtcatgta atctcttaat ttacttggat ctctcaatgg gtcttccatg
     2221 tactctttta atgcttctaa tttcatttca tattcaaatt catatctcgg attccaaata
     2281 tcacaaagtg tacgctcttt atcatacata gtaataggat ttccttgaaa ggaagttccc
     2341 tctacttttc ctagttcata aacttctttc ttcacgaaat gaaattgtaa ctgctgctct
     2401 tttgtaattg aacgatcaac gttatagcca tggggaaaag ttaagtgaat gaatttcggc
     2461 atattcgtag aaagctggta aatatctaaa gtcgtttcat gagagaagat tcccctggaa
     2521 aatcgttgct gaagccaaaa taaatcatct attccatgac catcctctac ataataacca
     2581 cgatcaactt tgatcaattt tccttgattc accaatcttt gtacagtggt tgctgcgatt
     2641 ccaaatttcc tggcttcctt catagataaa tttccatcgc gaatagccaa caattctttc
     2701 agtttatctt gcatttactt cccctccctt cgttacaaat acactacatc aaatatatca
     2761 tagtagttta tttgtaacaa gattaacgtc tatcgatcta attcgatttc tttttgaatg
     2821 cgctgctggt tctttaattg ttgcagtctt cttttatttg cacgtaattt ctcttgttct
     2881 aaacgaattt ttgctttttg attttcgatt tcttgttgtt tgtgtgttgt atttcgcata
     2941 ggataacccc ttcattattt tgtagtgcgg cgtgttcttt tcttatagtt cgtttttttc
     3001 ttttcagaaa attcaaagcc tgttgtaaag tcttctttta aaacgatcgt tgcttcaaac
     3061 gaacctttct gaccttttag tcctctgact gattggcttg ttcggccgtt ttgaatgatc
     3121 tctttcagtt gtgtggctgt gagtttcttt ttggcaatcg ttgtccataa tttaaaatca
     3181 caggcttcag acgtacatgc cgccacattt tgataaagca agacttcttg ttcccgacat
     3241 tttggacaaa cacctaaatg acgcgttgct tcctgttttt cactggctgc tttcacctct
     3301 tgtaacgttg atccaaaatc tgttgactgg atttgtcccg gtacgacttc caacaagtga
     3361 gagacaaatt tttggatatt agctaagaaa gtagcttgtg ttcctttctg ctcaccaatc
     3421 tttaataagt aactttccca tttggcggtc atttcggcac ttgttagcag cggctctttt
     3481 tccacggctt ggcaaagtaa aatcccttta tttgtcacca caagcttatt tttttctacc
     3541 ttgatatatt ccttttgttt caaagtttca ataatatttg ctcgtgttgc ttctgtaccg
     3601 attccttcta cttctttcaa tatggattgt gcttcttcgc tatccacggt ttttccagct
     3661 gttttcatcg ctgtgatcaa cgtaccttcg gtataaggtt gtggtggctt cgtttctttt
     3721 tcaaggactt gtagatctac cgtcaccgct tctccttttg tcactttagg aagtagcggt
     3781 tcttcttcct tttcttttct gtcgctctct ttgccaaaca gctttttcca acctagatct
     3841 actggtactt ttccgactgt ctttagctga agctgctgaa cttgcgtcaa gatcgttgtt
     3901 tcttcatagg tatacttttc cgcaaacatg gccaccgttg tttttaacac ttgttcataa
     3961 atagcctgct gcagtggact taattgttca aaagccgctg ccgtcatgac cgtctttgta
     4021 ggaatgatcg cgtaatgttc ctggactttc ttattattca catatcgact attcggtgta
     4081 tgaataggag taggaatggc ttcggccttt aaaaagtgtt tgtattcgtc taagtgatcc
     4141 aacagatacg catactcccc ttctgtaata taaggggtat ctgttcgtgg gtagcttaga
     4201 tatttccctt catacaaccc ttgcatggct tccaaagtat ctttggcact agctttcatg
     4261 agctgattca ttttagattg aaggctggat agagaaaaaa gacgtggact tgccgcactt
     4321 tttttctcgg tttcaacttg gtggatcgta cctggctgtt ttcccaattg aacccccttt
     4381 tcttttaaat aatcttcaaa tgcttcttgc gtcgcttggg tttcattcgg atcaagcttc
     4441 gcatcaaatg cgccattttg agcgattact tgggcttttc cttcaaaata tggttctttt
     4501 tggaagtttc taatcttttc ctgtaattga taaatcatgt aaagcgttgg ggtttggact
     4561 ctgcctaagg aaaaacttcc tggtatgcct ttttgctgca agagtaaact atacagcggg
     4621 ctaccgttca tgccgatcaa ccaatcggcg atctggcgtg cttgtgcttc ttcaaatttt
     4681 gggtaggtct cttctgcatc tcgtaactgc tggaatccct gataaatggc ttctttttct
     4741 aaggagttga tccaaagtct tttaaatatc tttccatgat cggcgcctgc ttgttgaatg
     4801 atcgtccaag ctatcagttc cccttcacga tcgctatctg tcgcaataat aatggtgtcg
     4861 gccgattgaa gttgctgttt caccaccttg aattgctttt tcttatcttt aggaacatga
     4921 tagtgataat gtgtagggaa aataggcaaa tgctctaatg accattgttt ccaattttca
     4981 tcgtacatat cgggcgaatc taactcgact aaatgaccaa aaccatacgt gatcacagtt
     5041 tctcctgaaa acaatcgatc ttggatctca aaatacccat cttttcgggt agctttagaa
     5101 aagctatcgg cataagcctt cgcttgtgac ggtttttctg ctaaaataac ggttttcatc
     5161 atgacgattt cactcccttt cttgctgtct accttgttga aacaacatgg cacgtttttg
     5221 ctttctgatc tcttgttggt agatttcatg cttcaattga tcattccaat ccttttttac
     5281 ttcaggtaca aattcatgtt catgattttt atagcctttg tagtattgtt ctgctttata
     5341 agagaaaaga atctgagcat taatatcccc tttttcttga taaaaacgat caaaatcgta
     5401 cgtaccatcc acgcgttcac aagcttttaa ttcttttgcg acttctctac aaatatcttt
     5461 caaattaata gcttctattg aatcgttaac agaaagctgc tgaccaaaag acgcatttac
     5521 tttgtgagcg gaaaccaatt ggttcgttaa ttgcagattg ttttctgtct tgtgtatggc
     5581 cataatagct tctggtgtga tcttttcttc ttttgcgact tcttcataaa tgactttgta
     5641 ttcttttggc acttgccaat cattagcgat tcctcgttca caacgtactt tctgattcgt
     5701 aaatggatca actgcgccca ttaattgttc tttttcgtag aaaatatgtc ctgcacggtc
     5761 attatccaca caaatctcga tggattgagg aaaggcttgt ttctttctca attcagatac
     5821 tgcctcacca aaataatgac tgatcacatg gtgttttaac ccttccatcg aaactaacca
     5881 cgtatcgttt aactgatcac gatttagtgc agcatagcta agtaaatcaa tactactctc
     5941 aaaaaatttt aactgcttag ggtcaccgat ctttaggtta aagccatgat tcgctgtcga
     6001 atttttatct atatgcttat atgtgcctct ctcaccaaaa cgttcataat caatgaacgt
     6061 tccttgataa ctcgccccta ctgtctcatt tgtctgtgga tctttccata aaaaggcgac
     6121 cgcatgatct aaacgatcgt ctcgaactaa gaaattttgc cgttcatgtt ttggctttag
     6181 ttcggcaatc aacccttctt tttctgcata atcaatcagc cactctggta gttttcgttc
     6241 ttccttcaga taattccttc ctttttctgt gagtggttga taatcaacgg ggtgatcttt
     6301 gtacttttct gtctcaaaat gaagattttc cactcgttct gttttgtctt cattctttag
     6361 aataaaatca gtggctgctt taaattgttg cttagggtct tggatctctt tgtctatgaa
     6421 aaaaagttta gctaattcaa taatgtcgcc actaatattt tgactgttcc aataaaaacg
     6481 ttgttttctt cgatcaaaaa caaatgaatc atggtcttct aagcgatagt ctcgtccttt
     6541 attgactaca gccattccat tgtttcgagc aaaatccact atatcgatct gtttacatcg
     6601 atcaataaga ctcatttttt tctcggtcat tttaaaacct cccaaaagaa aaaagactat
     6661 ccgtttttca gatagtcttt tttacttgtt ttatacacgt tctttttgtg acaattgttg
     6721 ttcataatag atttcatttt ttcgttttgc cacttctgac actgtctcaa gtgaaaatcg
     6781 gaatgaattt cctagcgcaa attcttctga aggtaacgcc gtttggatct cttttccata
     6841 actttttcct tttaaaatta cttgattatg ctgtggatct tcaataaacg ccgcatcctt
     6901 caaatgtaac gttcgacccg tatcttcttc cattactttc ccgtctttct tgtcgatcca
     6961 aagtgaacca acaggaacaa tattcttttg ttctgccgtt tctacagtat ttttatgctc
     7021 ctgcttcact tctgctggtt ggatctctgg ttctctttct ctgtattgat tgatttcatt
     7081 agaaatggtt tgaatgaatt catttgcgac acggctgaca tcattcatga ttttcccacg
     7141 ttccgcagct tccttatcat ccaacgtctg accattcttc gtccatgctg caatataagg
     7201 aatagctttc tccgaagtgt ccattccaaa atgtttgcac acaatataag aagtcatttc
     7261 tgcctgcaat tctttagttg cggtatctaa ttctgaaaat ttgtgcattc ttgcatgggc
     7321 taattcatga atacttgtag ctaaatgctg cgtaggagta tttcgagtat tcatgacgat
     7381 ttcttcttcg ccagtaaatt cgttacgaac atatgccccc cgtgcttgtc ctaattctct
     7441 ttgatagact tcactttctg tcatatctcg aatttgtatt ccaatattgt tggctaccgc
     7501 ttgaatccca gcttgaagtt ctgactgtcc ttttttatcc aattgaaaat caaagacacg
     7561 gttcggaaag attttcggat agtcttcagg ttttaactgt gtttgtgtga tatcaaacac
     7621 tttttcaatc gtgtagtaag gttttttgat cacttcgatt tgaccacttt cgattcgttt
     7681 tttatcttct ggtgtagctt ctgtttctcg aacaaagcca cgtcctttct cataaaatcc
     7741 tttgaccgtt tttctgacaa agatcttgtt cccttgttcc ccttttttga tatagattcc
     7801 ttcttttttc aacgcagcac gacctaaaca agccgttgca cctggaaatt gttcctcaat
     7861 caataattga tttctaaagg atcgttctgg aaaacgagac ataaaattca aaagttcttt
     7921 cacgtcttca ggagaatggg tatgcttttt caccgcatcc agtgcctgtt gcgaaaggga
     7981 tttgacttct tgcttaatct cttctgccgt tcgtttttga tagccctttt gttttgtttt
     8041 tgtttctttc gactgcattt gttctttcga aacaggttca tttttgaccg tttgtttttg
     8101 ttccttttca ggtacttgtt cgcacaattt tgctaactct aaatagatgg gttgattact
     8161 gttgacgcca tcccctagat ccattcggat ctgatcgact acttctccgt tcttcacttc
     8221 ttcaaaatag aatttataat aacctgcttc tgcggcgtat tggttatcca acgttttgag
     8281 ttgatcgatc aattctaatg ttacgatctc cccaccataa cttttcaatc ctttttctgg
     8341 atcattttcg ttgaattcta ctcgatagaa atgatcgttt tctttctcct tcaaagaatc
     8401 agttggcgca gcctcttcaa gtaattcacg aacagctgct ttttcctctt ccgacgtggc
     8461 ttggtatttt tttgattcct caaatccccc gttttcattc agttctaaag caacttttcg
     8521 atagtctttc aaaaaataaa tggttggatt ttttttccat tgcgtcactc gtccgctgtc
     8581 aataattgct tgtacttcag gaagaagatt ttcttttcct ttttctgatc tttctttcag
     8641 ctgttctaat gtttccaatt tccgttggta ttttcggatc gtagcggctg aaaaagtcga
     8701 ttcacctttt tcaaagcgtt cgatttcttc tttgattctt ggaatatttt cgattgtcat
     8761 atccaggccg ccgtacttgt tatagcccat ttcctttagc tcttcttgtc tttcaagttt
     8821 ttctacctgt ttttcttgtt gcttgatctc ttctctcaga ctatgcactt tgttttctaa
     8881 acgttcttgt tgcctaaacc aagaatgccc atttctctta tcgttcattg gctgaccgtt
     8941 ggtctttctc tggtgttcaa acatcttctc aatttgttcg tttaattcat tttgcaatcg
     9001 tactaatttt cttttggatt ttcccaagcg actattgtct agtggttctt tttcttctgc
     9061 catgtc
//
LOCUS       DACDRK010000089         8943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDRK010000089 DACDRK010000000
VERSION     DACDRK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.089"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(342..698)
                     /locus_tag="IWJ77_002405"
     CDS             complement(342..698)
                     /locus_tag="IWJ77_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9453962.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(712..1122)
                     /locus_tag="IWJ77_002406"
     CDS             complement(712..1122)
                     /locus_tag="IWJ77_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9453963.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1469..3655)
                     /locus_tag="IWJ77_002407"
     CDS             complement(1469..3655)
                     /locus_tag="IWJ77_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9453964.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3873..4556
                     /locus_tag="IWJ77_002408"
     CDS             3873..4556
                     /locus_tag="IWJ77_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9453965.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4637..5356)
                     /locus_tag="IWJ77_002409"
     CDS             complement(4637..5356)
                     /locus_tag="IWJ77_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9453966.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5668..5874)
                     /locus_tag="IWJ77_002410"
     CDS             complement(5668..5874)
                     /locus_tag="IWJ77_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453967.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6119..6721
                     /locus_tag="IWJ77_002411"
     CDS             6119..6721
                     /locus_tag="IWJ77_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9453968.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6862..8142
                     /locus_tag="IWJ77_002412"
     CDS             6862..8142
                     /locus_tag="IWJ77_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9453969.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8155..8883
                     /locus_tag="IWJ77_002413"
     CDS             8155..8883
                     /locus_tag="IWJ77_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9453970.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2598 a         1673 c         1470 g         3202 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
       61 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      121 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      181 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      241 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      301 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      361 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      421 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      481 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      541 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      601 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      661 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      721 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      781 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
      841 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
      901 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
      961 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1021 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1081 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1141 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1201 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1261 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaaattct
     1321 tgtcacacct cccttttccg aataaactgt gttccagaaa tagcttcttc gaaaataagc
     1381 tgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttctt tcttgaaact
     1441 gaccacttct gtcacaccct ctcttctatc actttccttt tttcacacga ctcccggttt
     1501 ctttgataaa tctcgatact ttcaattttc ttgaattttt catttgaggt acacatagat
     1561 acagacgatt tcttgctcta gtcatcgcaa cataaaatag tcttcgttct tcttctatcg
     1621 tcgtcactgg ttctgctttt atttcattaa agtcgattac tttcttttct gtcgctccgt
     1681 tataaggcaa acttccttcg ttacaaccaa tcaaaaagac atttcggtaa ctaagacctt
     1741 tacttttgtg aatgctctgg atacatagtg catcaagttt tccttgtctg taagcaatca
     1801 agcgggtttt taattcttta gctgtcgctt gggtttcttg cgataaggct tggagtcttc
     1861 tgaaagtagc atgctgcttt acttcttctc gtatccattc ttgatcactc tggtcgattt
     1921 ccttagctat acgtggcaga tgaagatagc tttgaacaag cgaatagatt tcttgatgct
     1981 ggtctgtttt acgcaacgct tcctcaaact ctcggatttc tgcagatttc gattttaaat
     2041 tggggcttat cgacaaaata taatctgcaa cggctatatt ttgatattta ggagaaaagt
     2101 attgatcgca ttcacttttt ttcaggtagc agtctgccat cagctgccgg agcaattgct
     2161 ggtaggcctg atttttcttt tgcagattca gtgctgtttg ccaatgaaca attttttgat
     2221 agaaatgttg gaaagacttg ataccaaaga taccttcata tggactatct gcttccagta
     2281 caaaaggaat attcgttttc agcagagatt ggacaagctg ctgcgtttgt gaagctgatc
     2341 ggtataatat gattgattcc ttatatttcc catttttttg aataaatgtc tgaatttgtt
     2401 tgattagcca ctgactttct gctttagtat cagaaaacaa ataggcttcc ataacacctg
     2461 cttctttttg tgctggaatc atcgcctttg gtatccgatc atttttatta tagctgatca
     2521 atctatttgc tccttggaca atagctgccc gagaacgata attagtcaat aagaaaagag
     2581 ttttggtagg atattctttt tcaaagttct tgatgtatgc tggctctgat cctctgaatc
     2641 catagatact ctgatcatca tctccaacaa caatcaactt gatcctgttt tcctttgtca
     2701 tccgtttcac gatttcccat tgaagtggat tagtatcttg aaattcatca atgaatagat
     2761 aatcatattt ttggccgata taatgctgga tctcctcatt ctccaaagcg cttttcagat
     2821 tttggagtac atcattgaac tctagcaatt gctggcttct tttttgttct tggagttccg
     2881 tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc attcttatca atggaatcat
     2941 atttttcttt aactaactga accatttctt ctactgaaag gttcatattc tgccaatagt
     3001 ctaaccgaga agcaatcgtt ttttctccat attgatcaaa tatataaaga ttatatttac
     3061 gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa aagtctcctt
     3121 gtattccttg cattgctact tcaggaaatt ttcgacaaat ttcattgtat atcctaaaaa
     3181 agatactatg gaatgttccg atatggacag cggtttttct ctggctgaaa ttatctactc
     3241 gtttttttag ttcatttgca gcgttccttg taaaagtgat cattagtatg cgttcttcgt
     3301 ctacttgttt ttgcgtcatc agataaacag cttttgcaca gattgtttgc gttttgccgc
     3361 tcccagctcc tgctatgatt cgaagatgtt tcttcatatc atatgtgata gctgcttttt
     3421 gttcttgatt gaagcgatta taggaacgtt tctccatcat tttaaagaaa gagctgtttc
     3481 cttcactttc ttttacttgt acttcttcct caataagtcc gggtgcttcg ccataagccg
     3541 cttcataggt agcaatgatt tcttcttgta gccgaaagac ttgttgaagc tttttatact
     3601 tgttctccca gcgtttcaat tctttttgta atcggagaat ttctttttca ttcaactgat
     3661 tgatttcctt cccatctttc ttctttgaat ttgtttattt caagttggtt atttaaagtt
     3721 tcgttgtcta ttttttcttt atactaacag acctttctaa aaatgaaaat gggaagcgat
     3781 atgattttag ttttctgaaa atttttcctt tttttcaatc gtttatgcta agataaggaa
     3841 gacaaaaata ttcataaaag gtgatgaaaa aaatgcagac ttcctttcac gaggggcgta
     3901 caataggact tttgctgaca tttatcggag gagccatgga cagttatact tatatccaat
     3961 atggtgcttt tgcctctgct caaactggca atatcattct atcgatcatc caagcatttg
     4021 acggagaatg gctaagtgtt ggtaaaaaag tattgtctac ccttttcttt ttcttaggga
     4081 ttcttctaac aaagtatcta attgattatt ttagaaaaaa agaaaaacat ttctggcgct
     4141 tgtttgttct atattatgaa gcagtgattt ttttctttgt tagtttgaca cccgtccatg
     4201 agcatcccgc attagtaacg attttgattg cttttactgc atcgatccag tgggtagctt
     4261 ttgataaaat caacgggcgt gcctacacta atttatttac aactggaaat ttaaaagggg
     4321 tagcaacgaa cttatatgat tattgtattt ctaaagaaaa agcagatttt gatcgatttt
     4381 tccactatct aagtgtcgtt cttgccttta tcgcaggagc tattgtcctt gttttctgtc
     4441 atcatctgtt tggcgcccat tcgattttga tcgtttccat tttatttttg atcatggccc
     4501 tcactcagac atttcattta tggcaatttt atcatacgaa tcggttcact atttgatctc
     4561 aatgaatcaa aaaaactaag caagatgctg ctcacagaga aatatctgcg agtgagcaat
     4621 ttgcttagtt tttatattat ttatttgtaa agattccctt tgctttcggg ttatttgtag
     4681 tgtagcggat cgactgggca tctgcctctt ctgtcatttt ctttgcccgc atcttgattt
     4741 cttgtacgat ttgttctcca catgccggat cggctacgaa atcatatttg tccccatcga
     4801 tcactatttt gggacatata tcaaactgtt cataccactc ttcgtatcgt cgattgagtt
     4861 ctttatagta ggaatagagt tcaggatctt gttctagctg ttcatattcc cggcctcttt
     4921 ttttgattct cgctaacata gtttcaaagg atacacgaat ataaaccagt agatctggtt
     4981 tcttttgtgg cgaaatttct tctaactcct tcagcatcgt ttctaaaaga ccttcatact
     5041 gctttacttc tacttcagaa acacgcccta aatctgcatt caaatgaaac agcatactat
     5101 cttcgtagat ggagcggtct agtacgttgt catcatggct gaaagcatct ttgattgcca
     5161 aaaagcgttt gttcaaaaag aaaatctgta ataaaaaagt atacttttct ggattagaat
     5221 aaaatagcgg caatacttca ttatcttcca cgttctcata aaaaggtttt gtttctaaat
     5281 cttgggcaag catttcagtt agtgtgcttt tccctgcacc gatcgttcct gcaacaatga
     5341 tcacaattat cggcatcctt cctgtaataa aatctgttct tcattttact acttcattta
     5401 tagaaataaa agccttgact ttatctgctc cttaaaagct acttctctaa aagtagccaa
     5461 aagtaactcc tattcatgaa aataaaggct gtgacaaaag tcttgtcgca gccctttttt
     5521 caaataaaca gtattctcct gccaacacct cggtcacaag gcaatattca taaaacatga
     5581 aaagctgttt tttaacatta ttccttgtaa ctcagagcta aacgggagac tcacaacctc
     5641 tttatgctct caacatttta tctgttttta ttcttctatt gtacttgatt tgcgtacaac
     5701 taactgccag tgcagattat gagcggtatc attgtcttct actgcgcatt tcatgacacg
     5761 tatcatcaca ctattttcat agattttttc tatttttcct tttattggtg attcaaataa
     5821 atcagattta aatgcatatg tttcgccgac ttcaaacaat tgttcatttt ccataagaat
     5881 ccctccatca ccttttataa ggtcagtaat tttttaacat aaatcactcg aaaaagcaca
     5941 aacttgtcat aaaaagtact tttcgtatca aaactgtcac tttttttggt aaggttcttg
     6001 gaaaacgaag atttttttag gagatcatga cggttttgat gtcaaaacca ctttttatga
     6061 aacaaaagac gttttttatt ttttttagca taaaatttgt ggtggaggta cgtatgagat
     6121 gaaagataaa gttgtgaaca aattattaga agccttattc agcggagaca aatcggaaga
     6181 agatatggtg tatgtccaag taaaatttgc cttagaagtt ttattgaata atcttggaaa
     6241 actagcggtc gttctagtct tttctctggt gacaggctca tgggcggaaa caggaatcac
     6301 ctttctttct tatatctgca tccgcagata tgcttatgga ttgcattccg actctgaatt
     6361 cgtttgtctg ctgtggacac tactttattt atggggcgtt cccttggtga tgaaacatct
     6421 gcaattgact atctccttcc caatgatggt ctttttgtta atcagctgct ttttgttact
     6481 gcttcgctat ggatcaagag gtacagcaat caatcccatt gaacccgaga aacgtccccc
     6541 attattaaag aaggcgatca gcatgtttct aattttttct ctcattactt tatttttctc
     6601 agcttcttac ttttctacct atttgctact tgggattgtt ttagaaatag ctacgttgct
     6661 tcctattaca aactacttga tgaattttgg aggaatcttt catgaaaaaa acaatcatta
     6721 aacaattaac taacttatct gacttttttg tatctggctg ctgctttggt atgagttctg
     6781 aattcaaaat ccctgaaaaa ttaaaaaaat aatacgctaa ttgtgctata atttggtaaa
     6841 aatcaatggg agctgtactt tatgttttta tggtccatag cgttgcaata tttgatttac
     6901 acaatttgta tcttattgat tgaccatcac atccgataca aatatctgtt tgcgggagtc
     6961 attctgattt cttgtatttt gattggatgg gtctattcga ttttagggat ttttactgct
     7021 ctaattgcta taggaatgct gatgcttact tttttcttct tttcgacttc gcgtcaacaa
     7081 ttggtttacg cgcttccttt tgggttattg acgtcgcttt taggagatca cttaacaagc
     7141 atcgacgatt tttttgtttt gaaatcttcg gttattgaac caggagattc attgcagtat
     7201 tttcacattg tcttatccgc ttttttatct gctttattag ctttttgttt aaaaaaatcg
     7261 ttagagactt ggtttcacac tgtggaccgc cgtatgatcg gtacgatcgg gacattgata
     7321 ttatttacat actatatcat tattttttat acccgttttt ctggtgaaac acctgatgtc
     7381 ttagctttga atacgagttt tttcattcta tatctgtgtg ttggcctgat catcttggtt
     7441 tattattgga aaatctctaa taagaaatta gctgatcagt tattagagca acaagtcagg
     7501 atgcaacagg attatatcaa agatctagaa aaaaactatc aagacttgag agaattcaaa
     7561 catgattacc aaaatctgct tttttctctt cacagctata tttcagaagg cgacttagaa
     7621 ggattacggt cttattatga tcaaacgata ttacctacac gccagatgat gaaccttttt
     7681 ccggccaatc taagtatgtt ggataatatg aaagttcctg agatccgtag tttactgtca
     7741 ttaaaactca tgatggcaca ggaaaaaggg ttgatcgtcc agcttcattt tcctgagcca
     7801 atcgaactgg atacgaaaca tacggtaaac cttgtacgta tgcttggtat tatcttggac
     7861 aatgcgattg aaggagctag tgctgcaaag aagaaaaaaa tcgaattgac catactcaag
     7921 aagaatcatt ccgtgatgat tcgtgtgatc aatacaacaa caaatacatt accgttaaac
     7981 cagttgaaac aaaaagggtt ctcaacaaaa acaaatccaa aaaacgaagg ccttggcttg
     8041 tttatcttgg atgaattgac aaagaccaat ccttatcttt tccttgaaac atcaattgaa
     8101 aaccagcgtt tttctcaaac gatttatata caaataaaat aaaggagtaa tgatatgatt
     8161 cccatttata tttgtgaaga cgatccaaaa caattagaag ttttatcaac ggtgatcaaa
     8221 aatagaatca tgattgaaaa cttagacagt tatgtccata tttctacctc tgatccggaa
     8281 gaacttctca atgcagccaa gatcagaact tcttcttttg gtatttactt tttagatatc
     8341 gaactagaag acagtgaaat ggatggtgta gcgatcggta aattgatccg tgaattagat
     8401 cctttaggaa aaataatcta tgtgacttcc catacggaaa tgtcgatgaa aatcttaaaa
     8461 agcaacatcg aaccgactga ttatatcgtc aaagaagatc tttatgattt aaaagaaaat
     8521 gtcgagcata tcctgagcaa aattttcgaa gattttcaaa tgtcccaaat ccaaaaagat
     8581 atcttcaaaa tagagtttaa tgatgagatc aaatttttac cgatcaaaga catccaatat
     8641 ttttctactt cgctaggagc gccacataaa ctggaagtcc atttgactca tgcccaatta
     8701 caattctatg agaagatcaa agaaatcgag aaaatgcatc ggaattttat ccgttgtcat
     8761 aaatcctatg ttatcaatac acaaaatgtg cggtcgatta ataaaaaaac cagagaagtc
     8821 acgttggcaa acggcgagac catcccagcc tctatccgtg gattaaagaa attgatttca
     8881 taaaaagaac cgaatagttt ggaccgacta aatgaccagc cgatccaaac ccttcggttc
     8941 ttt
//
LOCUS       DACDRK010000090         8919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDRK010000090 DACDRK010000000
VERSION     DACDRK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.090"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="IWJ77_002414"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="IWJ77_002414"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9453971.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IWJ77_002415"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IWJ77_002415"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9453972.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4432)
                     /locus_tag="IWJ77_002416"
     CDS             complement(3191..4432)
                     /locus_tag="IWJ77_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9453973.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="IWJ77_002417"
     CDS             complement(4450..5373)
                     /locus_tag="IWJ77_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9453974.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="IWJ77_002418"
     CDS             complement(5594..8086)
                     /locus_tag="IWJ77_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9453975.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="IWJ77_002419"
     CDS             complement(8093..8560)
                     /locus_tag="IWJ77_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9453976.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8696..8769)
                     /locus_tag="IWJ77_002420"
     tRNA            complement(8696..8769)
                     /locus_tag="IWJ77_002420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8733..8735),aa:Arg,seq:acg)
     gene            complement(8827..8900)
                     /locus_tag="IWJ77_002421"
     tRNA            complement(8827..8900)
                     /locus_tag="IWJ77_002421"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8864..8866),aa:Pro,seq:tgg)
BASE COUNT         2478 a         1884 c         1485 g         3072 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc
     8701 ccaagaggag tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
     8761 tatgggcgca tattcaattt aaaaaaatta taaatcattt gaaaacaaaa agcaagataa
     8821 aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     8881 ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DACDRK010000091         8857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDRK010000091 DACDRK010000000
VERSION     DACDRK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.091"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            331..1158
                     /locus_tag="IWJ77_002422"
     CDS             331..1158
                     /locus_tag="IWJ77_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9453977.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1431..2201)
                     /locus_tag="IWJ77_002423"
     CDS             complement(1431..2201)
                     /locus_tag="IWJ77_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9453978.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            2419..3486
                     /gene="ulaG"
                     /locus_tag="IWJ77_002424"
     CDS             2419..3486
                     /gene="ulaG"
                     /locus_tag="IWJ77_002424"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9453979.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            3504..3965
                     /locus_tag="IWJ77_002425"
     CDS             3504..3965
                     /locus_tag="IWJ77_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9453980.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3985..5469
                     /locus_tag="IWJ77_002426"
     CDS             3985..5469
                     /locus_tag="IWJ77_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9453981.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            5512..5811
                     /locus_tag="IWJ77_002427"
     CDS             5512..5811
                     /locus_tag="IWJ77_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9453982.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            5826..6464
                     /locus_tag="IWJ77_002428"
     CDS             5826..6464
                     /locus_tag="IWJ77_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9453983.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            6469..7329
                     /locus_tag="IWJ77_002429"
     CDS             6469..7329
                     /locus_tag="IWJ77_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9453984.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            7319..8029
                     /locus_tag="IWJ77_002430"
     CDS             7319..8029
                     /locus_tag="IWJ77_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9453985.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            8261..8695
                     /locus_tag="IWJ77_002431"
     CDS             8261..8695
                     /locus_tag="IWJ77_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453986.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         2945 a         1375 c         1861 g         2676 t
ORIGIN      
        1 aataaacatg agaacataaa aaaataaaat aattatatta attgataggt gtgctgtcgt
       61 cattatacac acgtaagaaa gaaatagtta tgtttaaaaa tattcttaga ctttctcgcc
      121 atgacaattt tcactatgtg ttataactgt aaacgataat tattcagata acacaaaaag
      181 aaatcaagga gcagatacgc cagttgttta atttgaatat tattttaaaa ttaaaatttt
      241 tattctgttc taaacatttt tggttgttta gaactttttt ttgtgtatga tataaaggag
      301 aaataatttt ttaaagggaa ggaaattgtt ttgattaaag tagatggagc attactaaaa
      361 gaataccgaa agaaaaaggg gttaacacaa gtacaattag cagaaggcat ttgtgaacaa
      421 gcaactgtca gcaatatcga aaacaagaat atgtcgccag gcatcaatat cttaagtgcg
      481 ctttgcaaaa agctgaatgt cgagatctcc gatatattag tagaaacaga agaagacaaa
      541 atcaggaaaa aaataaatga gattcaatca ttgaattctg tatcaaaata ccaagaatct
      601 tataaaattt tacaaacgat cgatcaggaa aaagtaagta attctgatct tttaaatcaa
      661 attttatttt atcaaggact gcttgatcat ctttatgcga aaaaaccaga tgcagcatta
      721 ttttatttta atcaagtgtt ggaacaaacg accgaaacag atatccatca cttacaagca
      781 gctgcaaacg tggcaatgat ctacttgtca aaaggagaat tagattttgc gaaagtttat
      841 gtggagcgta cacttaaaat attgagtgag actgattttg ataatctgat ggtctgcatt
      901 gtatattatg atatcgccac ctattaccgt aaaatcgagg attatgataa agcaatccaa
      961 ttatgtgaga agggtattga gtataataag aaacataaat cgacttatgc attggaatac
     1021 ttgctttatg aaattgcttc ttgccataag caattaggag aagatgatta tttagaaaga
     1081 tatatggacg caaaaaaaat agcgcgtttc aacggaaatg attacgcggt aaaggtgatt
     1141 gaaaacgacc tgaaataaag tggttgtgaa atcagtgttc tgatttgaat accgtttatt
     1201 cgaaaaaagg aggtgtgact tttgtcatac ctcctttttt cgatattgtt tgattcctat
     1261 ttaggcaaca tataacggtt ataaaagaag caatagatta tgatggtatc gattggatgg
     1321 atacatcttg ggattccttt atcaatttga gcgtagcgtg tttttcattt atcgataaat
     1381 ttgttattca tttttttgat aatcctattt acatatttac gtcctggttc ttaaaatgaa
     1441 tctacttgaa tggtttttat ccccgtattt ctgatctcct ctaatacttt ttcagaacaa
     1501 aatttatcag tgatcaaata atcaaagact gaaatatcag caatcttgaa ctttgatgtc
     1561 agtccgattt ttcgatgatc agcgactaaa atcttacatt tgattgtctt gttgaccatc
     1621 atttcgttga tcgttgattc atttaatatc tttgttgtga ccccatgtac tacatcgact
     1681 ccgctacagc caatgataca aatgtctgca ttgactgaag aaatcgtatt taatgcgatg
     1741 tcgcccacta gtgcttcttt tggaaagcgc atctctccac cagttaaaat ataactacaa
     1801 ttatctttgt gcggagagta attgattttc acgttgtttg taatgactgt caaatggtta
     1861 gattgaagaa aatccagtgt tttcaacgca gtcgagctag aattgataaa cagcgtatca
     1921 taatcgttaa tgtatttact agcttctctg gcaatcgctt gtttaatcct ttcaggacca
     1981 gattgatcaa aatcagtctg gtttttgtaa ttataagaac aataaccgta attgcgtatg
     2041 atctcgtttt tttcttctaa taaatttaaa tctcttcgta tcgtactaat agatacatca
     2101 agggattcag ctaattcgaa agttgatagt tgttcagtct gctgaagcag ttccaaaatt
     2161 tctttttgac gtttttcaat attgacaagt gagtttttca tgacttttgc ctcctgaggg
     2221 acatcttgat ccatggattt atttgactag ttgttcattt aaacattcat atctgtcttt
     2281 attatactga aacaatcatt gattactgaa agaaaatcat ataaaaaaca aacggtcata
     2341 taaaagggtt ttcatggatg ctgatacaca ttaatattta cttgtaagtt ggaacaacga
     2401 tatgggggga gcaagcaaat gagtaaaact gtcaatgaag taacaaaaga aaagtggtta
     2461 gtagaaacat ttcctgagtg gggcacatac ttgaatgaag aaatcgaaac agaagtagtg
     2521 gatcctggaa cttttgctat gtggtggctg ggctgtacgg gaatttggct gaaaagtcat
     2581 gaaggaacga atattttatg tgatctatgg tgtgggacgg gaaaacgtag tcatgggaat
     2641 ggcatgatga aaaaaggcca tcagatgatg cgtatgagtg gcgttcaaaa tatgcagcct
     2701 aatttacgta ctcagccttt cgtaatcgat ccttttgcga taaaaaatgt agacgcttta
     2761 atcgttacac atatccattc cgatcatcta gatatccata cagccgcagc agttcatcaa
     2821 aattgtcctg aagcaacatt cattgggcca aaagaagtgg ttcgcacgtg gcaaaaatgg
     2881 ggaattcctg aagaaaaaac gattacagta aagccaggtg atgcagtcaa aatcaaagac
     2941 atcaatgtag tagctttaga agcgtttgat cggacagcgt tggtcacttg tgaagatcca
     3001 gaagaggtat taaaaggaaa aatgcctcag gagatggatc aaattgcagt aaactattta
     3061 ttcgaaacat caggagggaa tctttatcat gcaggagatt cacattactc caatttattt
     3121 gccaaacatg gtaatgaaca caaaatcgat gtctgtttag gggcctatgg tgaaaaccca
     3181 cgagggataa cagacaaagt tacttctgtt gatatgctgc gcatggcgga gtcattgaat
     3241 gcaaaagtag tgattcctgt ccactacgat atttggtcta attttatggc tgatccaaaa
     3301 gagatcactg aaatttggaa attcaaaaag gatcgcctga agtatggatt taaaccttat
     3361 atctggcaag taggcgggaa attcatctat ccgacaaaca aagatgatct tgagtttaat
     3421 ttttatcgag gatttgaaga tgtcttctcg attgaaaatg atacaccgtt tccgtcgttt
     3481 ttataagaaa gaaggggagt ttcttgctga aatacttttt tgaccatcaa ttgattcatt
     3541 acccagataa agaaatcacg gattggcgtg aagctatcgc cgaaagctgt cttgtattga
     3601 tcgataagga aattattgat gaaacgtatg tggaggagat catccattgt gtagaaaaat
     3661 acggtcctta tattgtgatc gcacctcaag tagcaatgcc gcattcttca gaaaaaagtg
     3721 atggtgtttt tggaacagct atttcattta ccaagttgaa acagccaatc cgttttgaaa
     3781 gtgaagggga gaatgaggag aaaacggcta gtttgttttt tacactcgca gcaaaagatc
     3841 cagaagaaca tctagaaaat atccaaaaat tatccgaatt attaatgaca gatggattgg
     3901 ttgagcgtct catctctacg aactccatcg atgattacca agttgtgatg gcagaatata
     3961 attaattgat agaggagaaa aaagatgggg gaatttttgt taagcatttg gacgtatttt
     4021 gcaacaaata tcttgacgca gccagcatac ctgattggtt ttatcgtgtt attaggctat
     4081 attttgctga aacgcccgct tcatgaatgc atcgctggat tcttaaaggc aacggtcggt
     4141 tactttattt tgactgttgg ctctggtggt ttggtcaata attttcggcc aatcttggtt
     4201 ggactaaagg aacggttcaa tttggatgcc atggttacag atccttattt tggacaaaat
     4261 gctgtggatg ctggattaat ggagacattt ggccgtacct ttggagatgt gatgatttta
     4321 ttactgattg catttgtttt aaatatctta ttagtccgtt ttcagaaata tacgaaactg
     4381 cgttccgttt tcactaccgg aaacgttcag gttcaacaag cagctaccgc tttttggatt
     4441 ttactgtttt gctttcctga tctaggtagg atacaagcat tgatttttat gggactgatt
     4501 ttaggttgtt attggacagt agcttctaac ttaacagtag gaatcacgca ggaattgaca
     4561 gaaggtgccg gatttgcagt ggcacatcag caaatgttcg gaatctttat atttgcaaaa
     4621 ttagctgagt ggatgaaaaa gcgggatgaa aagaaaaata gatcaatcaa gcaagacaaa
     4681 aaaattgaag atatcaaatt accaggattc ttgtcgattt tcaatgagaa tatggttgct
     4741 acttcgttac tcatgctatt tttctttggg atcattctga ttgtactagg caaagattat
     4801 ttgattcaag cgcaattcat gcaagaaggg cagagtttct tattctacat tatgacgact
     4861 tctttaaatt ttgcggtata tctggcgatc ttgcaattag gtgttcggac attcgtcgat
     4921 gaactaactc aatctttcca aggaatctca aacacgattt taccaggagc tgttccagga
     4981 attgacgtag ctgctacttt tggctttggt tcgccaaatg cggtaacgat tgggttcttg
     5041 tttggtgctt tgggacagtt tttgatgatt ggtttactga ttttattgaa atcaccaacg
     5101 atcgtcatag ccggtttcat tccgttgttc tttgataatg ccgttatagc agtttatgcc
     5161 aataaccgag gtggattcaa agccgcttgc ctgttcccgt ttctttcagg aatcatacaa
     5221 gtaggcggtt cagcattatt cgctacatgg attgggatgt caacatacgg tggttatatc
     5281 gggatgttcg actgggcaac agcttggcca ttgtttacag taatcatgaa agtcctaggt
     5341 tatgctggaa ttgccgtagt tgtcattggt ttattggcta ttccgcagtt acaatatcgg
     5401 aaaaatcctg atggttattt tttgattgta gaagactatg acacatatgc agaaaagcag
     5461 agagtataaa agaaaatctg attagacaac actcataatg gagggattaa gatgagaatt
     5521 ttagtatcgt gtgcaaatgg atcaggaaca agcttgatga tgatgagaag tgtggaaaaa
     5581 gcaatgaaag aactaaacgt accaattacc aaaatccatc attgtgccat ttcagaggga
     5641 aaaagttcag ctggccaata cgatgtagtt tttacgccag tcaatttttt gaatatgttt
     5701 actcaggcag agaaaaaagg agttacagtt atcggtatca aaaatgtgat gtcagtcaaa
     5761 gaaatcagtg aaaaatttgc tgaaacaaag tggtaccaaa aaaacaagta aaggaaggaa
     5821 ctaagatgag caggccgaat ttgcaaattg cgttagatca caatgactta gaacatgcat
     5881 taggagatgt tatgaaagtt ggtgatgtag tagatattat tgaagtgggc acaattcttt
     5941 gcttacagga aggacaaaaa gcgattcgct gtattcgcag tatgttccca gataaaaaac
     6001 tagtcgctga tacgaaatgt gcagacgctg gaggaacagt ggcgagtaat gttgcaaaag
     6061 caggagcaga ctggatgaca gtcatttgtt gtgcaacgat tccaactatg gaggcagcac
     6121 aaaaagaaat aggtgaattg caagtagaac tctacggcaa ttggactttt gaacaagcca
     6181 tggattggca taacatcggt atccggcagg taatctacca tcaaagccgt gatgcgctgt
     6241 tatctggtga aacttggggt gaaaaagatt tatcgaagat caaaaaactg attgaactag
     6301 gctttaatgt ttctgtaact ggtggattaa atccacacac attacactta tttgaaggga
     6361 tcgatgtgta tacctttatc actggtcgtg gtattacagc agcaaatgat ccgatgaagg
     6421 ccgcacaaaa cttcaaagat gagatcattc gtatctgggg gtaaggaaat ggctcaaatt
     6481 gggatttatg aaaaagcgtt acccaaagat atttcatgga aagaacgttt ttcccttgta
     6541 aaagaaatgg acttcgattt tatcgagatg tcgattgatg agacggatga gcggttagca
     6601 cgattagatt ggtctgaaga aaagatagct gagctgagag aagagatgtt cagctccggt
     6661 gtacgtatcc attcgatttg tttaagtgcg catcggcgat ttccgtttgg ttctgctgat
     6721 ccagaaaaga aaaaagcagc gaaacaacta atgaaaaaag caatccattt ggcgcataat
     6781 ctaagcgtca aagtaatcca aatagctgga tacgatgttt attatgaaga gaaatcaatg
     6841 aattccagag aagactttat cgaaggaata aaagaaagcg taaaggaagc ttctaaatat
     6901 ggcatcattc tttccgtgga aatcatggat gatccattta tgaactccat ttcaaaattt
     6961 ttagaaatca aagaacagat tcattcacct tttctacaag tatatccaga cctaggcaat
     7021 ttgtccgcat ggccggaaaa taatccggct cgagaacttg agaaagggat cgattatatc
     7081 actgctatcc acttaaaaga tacttaccct gtaacaaaag aatattcagg ccagtttcgt
     7141 gatgttcctt ttggtagcgg ttgtgttgat tttcttggat tattaagaca tttgaaacgt
     7201 ctagattatg atggaacatt tctgattgaa atgtggagtg aaaaaagcca agactttcga
     7261 agagaaatcc agcaggcaaa agactactta agttctaaat tgaaggaggc tggctatgat
     7321 gtcttgtgaa gaaaaaattg cagcaatgaa gcaacgtgtc cttgaagcaa atctggcatt
     7381 gccgcgatac ggattagtca aattgacttg gggcaatgtg agtgaagtgg accgtgagct
     7441 aggagtcatc gtcattaagc caagcggtgt cagatatgaa tgcatgcaag cagatcaaat
     7501 ggtggtaaca gatttgtcgg gaaatataat agaagaagat agcttaaaac catcttcaga
     7561 tctccccaca catgttgttt tgtatcaaac ttttgaagat ataactgcta ttactcatac
     7621 tcattccacg cactccgtca tgtgggcgca agcaggacgc gatttgccag cttatggcac
     7681 gactcatgct gatgcttttt atggtaaggt tccttgtaca cgccaattaa cgaaagaaga
     7741 agtaagagaa gcttacgaag ttcatacagg gaatgtgatt gtcgagacgt ttaaggaaag
     7801 aaaattagat ccaaacgaag tccctggagt acttgtctat ggacatggac cgttcacatg
     7861 gggggattca cctatgaaag cagtagaaaa cagtttgatc ttagatgaaa tttgtctgat
     7921 ggccaaagaa aacgagctga tcaaccccaa tatatgtgaa atcccgcaat atttactaga
     7981 taaacattat tttcgcaaac atggaaagta tgcatactac gggcaataga gatgctaggg
     8041 taagggtaga aaattaaaag ataacacttg atagagagaa acagaggttg tgaagaaaat
     8101 ttatcacaac ctttttattt atcaaaaagt atgcattcta aaaggaaaga ggaattttaa
     8161 ggaatcgaga gaatctttac aggagagtta tatgtgatag attaaatagt caaagaactg
     8221 tattattaga gggagaattg tataagaaag gaagaaaaaa atgaatattg attcccttat
     8281 gatcgatgag atttctgaat tatataagag cgttggttgg acgaattaca caaaagacac
     8341 tgcaaggtta gctagagcat ttgaacaatc tgaatctctg ataaagcgta atggggaagg
     8401 gaagattatc ggaatcgttc gttggatcac tgattttgcg acgatcgcat ttattcaaga
     8461 tatcctcatt catccgaggt atcagcgcca aggaataggc aaagtgcttt tgaatgaagc
     8521 tttggagaag atcactagtt atggaccagt ccaaatcgaa ttattgacag atgatactga
     8581 gaaaacaaaa aaattttatg aatcagtagg atttgttcaa gtaaaggaaa tgggtggtat
     8641 ttcttatgtc atagatacta gaagaaaaaa ggtgcttttt ggtaatgaca aataacatat
     8701 aataacgtac aaaagaaaaa attctagctt agttctttag aaaaacaaga ggatagactt
     8761 agtatcactc aatctatcct ccaatgctca gctattttat ttttttactc acctctttat
     8821 caaatacaat tttttgcaaa ttcgaactaa tataaaa
//
LOCUS       DACDRK010000092         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDRK010000092 DACDRK010000000
VERSION     DACDRK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.092"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWJ77_002432"
     CDS             complement(<1..173)
                     /locus_tag="IWJ77_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9453987.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWJ77_002433"
     CDS             complement(593..1714)
                     /locus_tag="IWJ77_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP9453988.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWJ77_002434"
     CDS             2042..3451
                     /locus_tag="IWJ77_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9453989.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWJ77_002435"
     CDS             3448..4176
                     /locus_tag="IWJ77_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9453990.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWJ77_002436"
     CDS             complement(4304..5215)
                     /locus_tag="IWJ77_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9453991.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWJ77_002437"
     CDS             complement(5232..6239)
                     /locus_tag="IWJ77_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9453992.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWJ77_002438"
     CDS             complement(6236..8365)
                     /locus_tag="IWJ77_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9453993.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWJ77_002439"
     CDS             complement(8370..8477)
                     /locus_tag="IWJ77_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9453994.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDRK010000093         8163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDRK010000093 DACDRK010000000
VERSION     DACDRK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.093"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IWJ77_002440"
     CDS             94..1248
                     /locus_tag="IWJ77_002440"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9453995.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IWJ77_002441"
     CDS             complement(1270..2022)
                     /locus_tag="IWJ77_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9453996.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IWJ77_002442"
     CDS             complement(2386..5052)
                     /locus_tag="IWJ77_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9453997.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IWJ77_002443"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IWJ77_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9453998.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IWJ77_002444"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IWJ77_002444"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9453999.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2369 a         1695 c         1457 g         2642 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcg
//
LOCUS       DACDRK010000094         8006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDRK010000094 DACDRK010000000
VERSION     DACDRK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.094"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            69..770
                     /locus_tag="IWJ77_002445"
     CDS             69..770
                     /locus_tag="IWJ77_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9454000.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1379..2395)
                     /gene="tsaD"
                     /locus_tag="IWJ77_002446"
     CDS             complement(1379..2395)
                     /gene="tsaD"
                     /locus_tag="IWJ77_002446"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9454001.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2392..2856)
                     /gene="rimI"
                     /locus_tag="IWJ77_002447"
     CDS             complement(2392..2856)
                     /gene="rimI"
                     /locus_tag="IWJ77_002447"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9454002.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2863..3405)
                     /gene="rimI"
                     /locus_tag="IWJ77_002448"
     CDS             complement(2863..3405)
                     /gene="rimI"
                     /locus_tag="IWJ77_002448"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9454003.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3389..4213)
                     /gene="tsaB"
                     /locus_tag="IWJ77_002449"
     CDS             complement(3389..4213)
                     /gene="tsaB"
                     /locus_tag="IWJ77_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9454004.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4302..5282)
                     /locus_tag="IWJ77_002450"
     CDS             complement(4302..5282)
                     /locus_tag="IWJ77_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9454005.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5306..6790)
                     /gene="galT"
                     /locus_tag="IWJ77_002451"
     CDS             complement(5306..6790)
                     /gene="galT"
                     /locus_tag="IWJ77_002451"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9454006.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6802..7791)
                     /gene="galE"
                     /locus_tag="IWJ77_002452"
     CDS             complement(6802..7791)
                     /gene="galE"
                     /locus_tag="IWJ77_002452"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9454007.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2259 a         1714 c         1409 g         2624 t
ORIGIN      
        1 taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg
       61 actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg
      121 attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt
      181 cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct
      241 tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg
      301 actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca
      361 cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg
      421 aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag
      481 tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg
      541 taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata
      601 tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg
      661 aaactgctgg acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa
      721 agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa
      781 aataagcagt gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct
      841 gaaagatccg gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca
      901 gttatgtaat attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg
      961 tcctcatccg atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc
     1021 aatcagagga acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat
     1081 taaaagtatg taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa
     1141 cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg
     1201 ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta
     1261 aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa
     1321 gccgtttttc ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt
     1381 attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt
     1441 gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag
     1501 ggggaatcac tagttctacc tcagggagtt tagctgccaa agcatcctgc aatccttcac
     1561 gcaacccttg attggccgct acacctccag ctataatcag ttgcttgact ggataatttt
     1621 gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg
     1681 ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa
     1741 atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg
     1801 gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt
     1861 aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg
     1921 tctccccaat gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc
     1981 cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga
     2041 tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct
     2101 tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga
     2161 ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg
     2221 tgatttgttc tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat
     2281 gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg
     2341 ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat
     2401 cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata
     2461 tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc
     2521 attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag
     2581 taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt
     2641 tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc
     2701 ttcagctaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt
     2761 cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa
     2821 gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag
     2881 ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt
     2941 tgcttgaaac cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc
     3001 taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag
     3061 cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc
     3121 taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag
     3181 atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa
     3241 ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa
     3301 ttctcttagt acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt
     3361 atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag
     3421 gtgtgtgcgt ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa
     3481 aagcttgttt ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac
     3541 ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt
     3601 cagagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta
     3661 gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat
     3721 tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca
     3781 caataatatt atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta
     3841 ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc
     3901 ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt
     3961 gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga
     4021 tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt
     4081 ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct atgacattct
     4141 cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag
     4201 tacaataata catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa
     4261 agctgcggta tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga
     4321 ataatcaact ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat
     4381 tgaagaatcg tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa
     4441 agcgagggcg ttacatattt cgctacactg acatcattga agcccatcac agaaatatct
     4501 tctggtacca ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca
     4561 ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt ttaaatattg cttcactgct
     4621 tgatatcctg actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt
     4681 tctgccaaaa cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc
     4741 ttcgtatatt ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt
     4801 tcaatgactt tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa
     4861 gtgccgtcag aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga
     4921 gcttcgtcga attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc
     4981 cacgtttctt tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga
     5041 atagataaat aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga
     5101 agcgtttgct tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct
     5161 gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga
     5221 actcttgaaa cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc
     5281 atttgtctct cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct
     5341 gtcctttttc atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac
     5401 ctacagattt ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt
     5461 ttaattgttc tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga
     5521 aatgctctac ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta
     5581 cttcgatcaa cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa
     5641 tcccatcagg atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt
     5701 aattgtccct tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa
     5761 aagctctgat agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag
     5821 ctgcttcaat aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt
     5881 tcacgactcc tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg
     5941 cttttgccat aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc
     6001 ctccaacaat tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa
     6061 tccgaagcag tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata
     6121 aaatgatcga atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac
     6181 cgtctagatt cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc
     6241 tatatccttc gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc
     6301 gttctgctgc gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca
     6361 gttcaccata ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga
     6421 tgtagtcatt tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg
     6481 aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta
     6541 attgcgcttc aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg
     6601 ccttagcgac aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa
     6661 tgctttctag agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt
     6721 ccatttccat ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac
     6781 tgattgacat atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc
     6841 ccatgctgtt ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc
     6901 tctcgctttt tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat
     6961 ttctgctgga atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga
     7021 gtatcctttg ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc
     7081 caaagctaaa atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt
     7141 tccatctggt gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc
     7201 tacttgtaaa atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc
     7261 atctgctttt gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc
     7321 ttggtcacac cacttcatca ttttttccat catcaattta ctttcaccat acggattttt
     7381 aggattagct ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc
     7441 agtagaagag aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg
     7501 cataccgtat acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa
     7561 agagcttgca gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag
     7621 aaattcttta tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt
     7681 gtgacctgtg agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc
     7741 tacagcgtgc gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac
     7801 tcctttgatt agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact
     7861 tttatggtga aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa
     7921 acatttagta aacattttca agggtttttt gcaaaggttt acaaacaaac gtaaaactag
     7981 cctttttttc tcttttgttc cataat
//
LOCUS       DACDRK010000095         7858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDRK010000095 DACDRK010000000
VERSION     DACDRK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.096"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..179)
                     /locus_tag="IWJ77_002453"
     CDS             complement(<1..179)
                     /locus_tag="IWJ77_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9454008.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="IWJ77_002454"
     CDS             complement(192..1295)
                     /locus_tag="IWJ77_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9454009.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="IWJ77_002455"
     CDS             complement(1385..2320)
                     /locus_tag="IWJ77_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9454010.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="IWJ77_002456"
     CDS             2622..4556
                     /locus_tag="IWJ77_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9454011.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="IWJ77_002457"
     CDS             5013..5429
                     /locus_tag="IWJ77_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9454012.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="IWJ77_002458"
     CDS             5443..6009
                     /locus_tag="IWJ77_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9454013.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="IWJ77_002459"
     CDS             6032..7018
                     /locus_tag="IWJ77_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9454014.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7858)
                     /gene="scm"
                     /locus_tag="IWJ77_002460"
     CDS             complement(7173..>7858)
                     /gene="scm"
                     /locus_tag="IWJ77_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9454015.1"
                     /translation="SDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDT
                     TSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTT
                     STTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHAS
                     VSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRIT
                     FMSYKKSKR"
BASE COUNT         2230 a         1503 c         1639 g         2486 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     7681 tcacttgaag ctgatgtgtc cgtgctatca cttgtggtag aggtagtatc tgtactgtcg
     7741 cttgaagctg acgtgtccgt gctgtcactt gtggtagagg tagtatctgt actgtcactt
     7801 gaagctgatg tgtccgtgct atcacttgtg gtagaggtag tatctgtact gtcgcttg
//
LOCUS       DACDRK010000096         7510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDRK010000096 DACDRK010000000
VERSION     DACDRK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.097"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(190..867)
                     /gene="nth"
                     /locus_tag="IWJ77_002461"
     CDS             complement(190..867)
                     /gene="nth"
                     /locus_tag="IWJ77_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9454016.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(874..1572)
                     /locus_tag="IWJ77_002462"
     CDS             complement(874..1572)
                     /locus_tag="IWJ77_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9454017.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1840..3024
                     /locus_tag="IWJ77_002463"
     CDS             1840..3024
                     /locus_tag="IWJ77_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9454018.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3030..3473)
                     /locus_tag="IWJ77_002464"
     CDS             complement(3030..3473)
                     /locus_tag="IWJ77_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9454019.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3699..3986)
                     /locus_tag="IWJ77_002465"
     CDS             complement(3699..3986)
                     /locus_tag="IWJ77_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454020.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4157..5509)
                     /locus_tag="IWJ77_002466"
     CDS             complement(4157..5509)
                     /locus_tag="IWJ77_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9454021.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5782..6228)
                     /locus_tag="IWJ77_002467"
     CDS             complement(5782..6228)
                     /locus_tag="IWJ77_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9454022.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6464..7390)
                     /gene="cysK"
                     /locus_tag="IWJ77_002468"
     CDS             complement(6464..7390)
                     /gene="cysK"
                     /locus_tag="IWJ77_002468"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9454023.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2026 a         1542 c         1232 g         2710 t
ORIGIN      
        1 tttctacatc gggtattttc ccaataaaaa taacaacttt tatttttatc gcaataaatt
       61 gtaataagtt accatcctag ctactgagta tgtacattta ctttttcatg caaaaaagat
      121 aaacgaaaca gcttttctcc ataataacaa gataattcat acatttattt gcaattaaag
      181 aaaccacgtc tacaggcgtg gtttcttttt tagcgccttt tcttttagac gcatccgatt
      241 tttcccatct tgacatattg atagcaaagg acatacctcg cacttgggat ttcttgccgt
      301 gcaatgatat ctaccaaaaa aaatcagtgt atgatgtgtc tttacccata attcttgtgg
      361 tactttccgc atcagtgttt cttctacttc catgaccgta gcatctagtt tgcagatcct
      421 caggcgtttt gagacacgtt ccacgtgggt atctactgcg attgctggta tcccgaatgc
      481 atcccccaat acgacatttg ctgtctttct gcctacacca ggaagagaca tcaattcttc
      541 tcttgacgtt ggcacttgcc catcgaaacg ttcaatcagc tgctgtgcac acgctttgat
      601 gttttttgct ttatttcgat acaaaccaat cgtttttatt ttaagaatga tttcatcgat
      661 tgaagcttct gctaacgcat cgggtgtcgg aaaagaggca aacaaatctg gcgttgcttt
      721 attcactgat acatctgtcg cctgggcact taagatcact gcaatcaata actcaaatgg
      781 attattgtgc ttgagttctc catgtgcttc gggaaacatt ccatacatcg tttctagagc
      841 ttccattgtt ttttgtttgc ttaacatatt ccgctactcc tccaaccaat ttttcagtga
      901 gactttcggc aattcttctt gttgagctga ctgttctact tctctttgaa gaagctgctg
      961 ttttcttcgt ttttggtctt cttccacttg ttgttttgta cgtagatttt ttctttccca
     1021 cgatagcaaa atgcggtcaa cgtaattaaa actataagcc tgatttaata cggcttcccg
     1081 cagtgctagc ttcaaaattt ccggctgata atggtcttct tctagccatt gtccgatccg
     1141 ctggaactcg atcgaagaaa gcgggcgtcc aaattcttgt tcaaataatt gatacactga
     1201 ctggatttct ttttcttgtg ttttttcttc gtattttttt tcttgtgttt ttaaatactc
     1261 acctaataga tcatagatgg ggtaaagatt atatcgatct gcttttttac cgttgacaat
     1321 cgtcgtttct atcttgataa atccatttgt tacgagacga tccagtattt gaaaaatctg
     1381 atcttgtttg attcccatat ccatagcaat catctgcaaa tcagggaaag tatctccttc
     1441 aagctgtgcc atatgcaact gtagaacaaa taaaaattct tcagattgta gaccgatcct
     1501 atgataattt ttaagtaaaa gattagatat gactgtttgt ccagcgctta agtaattttc
     1561 taaattcaac atgatttctc ctccgctcaa tcacagtata cgtaaaaaaa acatgggaag
     1621 caagaacagg tttcttttct atttacttca ctgatcggtc agatttacaa tgtcgtctac
     1681 ttgatttttg tttgttcatg tttttgatgc gtatcttcac ttttcggaat aaataaatta
     1741 agtagatttc gctgttcctt agaatgttga caaggaaaca aaattactat acactatttt
     1801 tgtttccaag ttaacattat tgcataaagg agtttttaca tgtacagttt cacaaataaa
     1861 cattctaaag tttttggata cgtctctgtc ttattcactg gattaggatt tactatcgtc
     1921 agtcctgtac ttccgttttt aacgctgcct tattctcact ccatacatca gcaagctttc
     1981 tatatcactt tgctcatgtc tgtttatgct ctagctgcct tcttatctgc ccccattctc
     2041 ggtagcttaa gtgatcattt tgggcgtcat ccgatcctta ttatcagttt attaggatca
     2101 agtatcggat atttgctttt tggtttagga aattcgatat ggatgttgtt ccttggtcgt
     2161 atcattgaag gattgacggc aggagaaatc tccaccttat atgcttactt tgccgacatc
     2221 acggaaccta atgaaagaac caaagttttt ggatggatgg gtgctttagt cggtatagga
     2281 acaacgctgg gtcctattat agggggtcta ttagctgagt tggggaattc tgttcctata
     2341 tttattggag cactctttac tttcttaaat gccgtttatg gctacacatt tatgctagaa
     2401 agccttccaa taaaaaaacg ttcagtagat ttaagtttct cccatgtaag accttttcac
     2461 caattacaac aattattcaa gatttcatcc gttattcctt tattgacagc aggttttgct
     2521 gtttggttgg cagctggctc tcttcaatct atcttttctc aattttcgat tgacgccttt
     2581 cagtggaagg ctgggttggt tggtttaagc ttctctttga ttggaatctt ggatactgtc
     2641 tcgcaattgt tcattatgcc tcgattgctc aaaaaatttt cagaacagca gatcacaaga
     2701 atagggatgt tcagtgaaat cctcgcctat ctattcatca cattatctgg aattcttcta
     2761 ctgccgattt tgtttttatt tgggattatc tgttatggtt tcggtgattc tatttttact
     2821 cctgtattca atggccaact atccaattct gtctcagata accagcaagg tcttgtaatg
     2881 ggcggtaccc aaagtataca atctctatcg cgcgtcatcg gcccgctaat cgcaggacag
     2941 ctttatgctg cagcaccatt tcttccgacg gtattcggat ttttgcttat gatttttgcc
     3001 tattatcact acaataaaaa atagtagctt catttgctac ttcgtataaa ttctctgtat
     3061 cgaccgataa aacgaaatac tgtgtcaaat tcttcttttg acatattttc aaatacagta
     3121 gcatctcgct gtacccattt ttgatggagt tgctcgtgtc tattccttat tttttgtccg
     3181 attggcgtca attgaaaata gatttctttt ttattctctt cgctttgata agtgtttatg
     3241 atccctctag ctatcagttt ttttgttagc ttactgatgg ctcctctagt catgtaacta
     3301 gcattcgcta attttgtcac gttaggatca gggattttcc cgatatgttc aatcacttct
     3361 agttcattta gagtaaatcc tgcaaattct ttttccattt ctggtttatt acgatgaata
     3421 atctcattag ctaaatccag atattcttct attagcagtt gtcttttatc catttctaca
     3481 ccttcttttt ctttcattat attgtttttt tgtttcctat tcaacaaaga taaaaatatt
     3541 ttgtaggaaa acccaatctg aaaccaagaa aaaaaggggt tgacaaagct tgtcataacc
     3601 cctttttctg attaaaagat agtaaagaga cagtcatcgg taattgtgat gattttttgt
     3661 catcacttaa ggatgagcgg ccttttcaaa aactgtcttt aaagatcatc atcagtttct
     3721 agcacatatg gctgaccttc atatgtgtac tctgtattca atgtgccgtc attttcatct
     3781 gagtagatgc gtctactgaa aggaaaacga tgagaaacat tatctttggc gtcatttgct
     3841 ggcatcgtgt aatattcctc ttgcgtttca ttcaactgag agagtacttg atctgccaac
     3901 tcatcaatat tgatcgttaa tttcttcgaa gcacttttat aatcttctaa aaatgcatct
     3961 tgttggacaa aatattcttt attcatcttc tttctcacct ttcttttcaa aatcaaataa
     4021 agctgcttgt tcttttatgt aacagcttta tctttgctgc ttattgtgac aaaaaattcg
     4081 aagaaagaca actaattgta cttttcacaa aaaaagacag agggtcgtga caaaattgtg
     4141 tcacaaccct ttttctttat tgtgcagttt cgatttcgcc tagcgctgct tgtgcaagta
     4201 ggttcaagta attccatggg cggtcaaaat gtggttggaa gaagaaatct gaaagtgcga
     4261 gatcttctac agtcatttta ttttgaacag ctaaagacat ggtatttgct gattgtgtaa
     4321 tgtcgtattt ggacatgaac tgagcaccta cgatacgatt cgttcctttt tcatagacta
     4381 gctccatcat tactttttca gtagttggca tgaactctgg acgataatta tcttcaaata
     4441 cagtcgcttg gacttctaga tcgttcaaag ttgcgctttc tttggtcaca cctgtcgaac
     4501 cgatcgtcca gccaaatagg taaagtcctg aagttccttg agtaccacga tacgccattt
     4561 tatgatctgt caagttattt cccacaagca aaccttgacg aacggcattt gttgccaaag
     4621 gaatatagtt ttgcgtgcca cttggattgt agttgactac tgcagagtct ccagctgcaa
     4681 aaatgtctgg attacttgtc tgcatgtatt cattcacttt gattgccccg tttggcagtg
     4741 tatctacttt tccttcgatc agttttgtat ttggacggaa gcccacacat aaaatgacca
     4801 tatctgtatc aaatgtatct gttggtgtag ctacttgttt cacttttcct tcttcatctg
     4861 tgataaattc agtaacattt tcgcctagcg caagagtaac accgcggtct gtcaattctt
     4921 tttctaagat atctgtgaat ggtttatcta gatatttatt caagatgcga tctaatccat
     4981 caatcaatgt cacttgtttt ccagattcga caaaagcttc tactaattca ataccgatat
     5041 atccaccacc aacaattact acacgttttg cttctttcgc ttgtgcaata atttcatttg
     5101 cttgattgta gtttttgcat aggagtacat ttttcgcatc gattccacta atcggtggga
     5161 taataggcca tgaaccagtt gtcatcacta atttatcata agaaaccgtt tctgtttctc
     5221 ctgttttcag atcttttgcc gttactgttt tattttctgt atcgacgtct gttacatcgt
     5281 gttccatttt aactgttgct cctaatgaag ataattcttc aggatttgag tagaacaagc
     5341 ctgctgggtc ctttactact ccgccgacat aaagtgcgat accgcaagat aagaaagaaa
     5401 tattgtcatt tctttcatat actgtcactt ctgcttctgg atgatttttt aaaatacttt
     5461 ttacagctgc agtacctgca tgagtacagc caattactac tactttcatt tatggtccct
     5521 ccgattaaca ttttgtcctt cgacaaaact actataacag aaataaaacg gatacaacag
     5581 tttaatgctt gtgaattcac aaaggtttgt ttgtagtttc attccttttc aagcataaga
     5641 acccaatatc tacaagattc ttattttaat aatattcact tttatatcta agattgtgaa
     5701 taaaagacaa aaaaataaaa aagctagcgt tttttttaca cagctagctt tttttaggta
     5761 taacacttgt agaaactgtt tttacatttg cgcctttttt gctgactctt tacaatccgg
     5821 acagagtcca tacaattcca accgatgttc attaatctca aaatcagtca gtctgcttgc
     5881 agccatttct atatcttcaa gccctgggta atgaaaatca acaatcttac cgcagttttc
     5941 acaaattaca tgataatgac gctttgaact gaaatcaaat cggctagaag catcgccgta
     6001 cttcatttct tgaacaaatc caatcgaagt aaataagcga aggttattgt aaacagtcgc
     6061 aacactcata tttggaaacc gatgttcaag tgctttataa atctcatcag ccgttggatg
     6121 ggaatgatgt tcaatcaaat attctaaaac cgcgtaccgt tgaggtgtga ttcgaatgtt
     6181 ctcttttttt aacttttcga taacctcttc gactaactta ttttccatgt aaacacccca
     6241 cgttgttatt ctcgtcttat catactattt ttacatttta aaatccactt aacatgaaaa
     6301 gaaaattcaa ttttattgta ttttaatgaa aaaagacgta aaacagtcgc gttcactatt
     6361 ctacgtctct attcaaataa tttttctcat cataaatttt tgtgtgttct tttcatcttt
     6421 aaaagttttt tacataaacc tatctggaga aaaaccaagt gccttatatc tcttggtaaa
     6481 gggctgtaga taaataacgt tcaccattgt cgggaaccac agctaatacc ttcttgcctt
     6541 ttcctaattc ttttgcgact tttagcgcgg cagcaattgc agcgccagat gagatgccta
     6601 ctaaaatccc ttcttttcgc ccaacttctc tagctgtttc catcgcttcg tctccactaa
     6661 tagaaaggac tttatcatac acagaagtat ccagtgtttt aggaacaaaa cctgtaccga
     6721 ttccttggat tttgtgaggt cccgcttctt tcccttctaa aatagcagat tctgctggtt
     6781 ctaccccgat taattcgatt ttcggataca cacgttttaa ttctcggcca gcgccagtga
     6841 tcgttcctcc tgtaccgata cctgcaacaa acgcgtccaa gccatttact ccaaatgctt
     6901 gatgtatctc aggaccagtc gtcttctcat gcacaagagg atttgcttca ttttcaaatt
     6961 gtaatggtaa aaagtagccg ttttcttttg ccaaacgttc tgcttctctg attgatccag
     7021 aaataccatc cgcaccagga gtcaaaatca actctgcgcc gtaccctttc atcaataaac
     7081 gtctttcgat actcatcgtc tcaggcatca cgatgattac tttatatcct tttgcaactc
     7141 ctaccatcga caatccgatt cctgtatttc cagaagtagg ttcaatgata gtatcacctg
     7201 gtttcagtag accatcgtgt tctgcttttt ctatcatact taaagcaata cgatctttta
     7261 cacttcctcc tggattaaaa aactcaagct ttacaaaaac atctgctgaa tcttctggta
     7321 cgattttatt taatttgacg attggcgttt tgccaatcaa ttcggtgact gagttataga
     7381 tagctgtcat cttcaattcc tccttgtctt tgctcttctt acttatcata atacaatctg
     7441 ttcatttatg gcgagtgatt cgtctttgac tagcaaaaaa aagacaaaga tataccatat
     7501 atggtaattt
//
LOCUS       DACDRK010000097         6740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDRK010000097 DACDRK010000000
VERSION     DACDRK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.098"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(82..495)
                     /locus_tag="IWJ77_002469"
     CDS             complement(82..495)
                     /locus_tag="IWJ77_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9454024.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(951..1436)
                     /locus_tag="IWJ77_002470"
     CDS             complement(951..1436)
                     /locus_tag="IWJ77_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454025.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1575..1790)
                     /locus_tag="IWJ77_002471"
     CDS             complement(1575..1790)
                     /locus_tag="IWJ77_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9454026.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2014..2814
                     /locus_tag="IWJ77_002472"
     CDS             2014..2814
                     /locus_tag="IWJ77_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9454027.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2833..4701
                     /locus_tag="IWJ77_002473"
     CDS             2833..4701
                     /locus_tag="IWJ77_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9454028.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4694..5185
                     /locus_tag="IWJ77_002474"
     CDS             4694..5185
                     /locus_tag="IWJ77_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9454029.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5173..5727
                     /gene="srlA"
                     /locus_tag="IWJ77_002475"
     CDS             5173..5727
                     /gene="srlA"
                     /locus_tag="IWJ77_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9454030.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5745..6740
                     /locus_tag="IWJ77_002476"
     CDS             5745..6740
                     /locus_tag="IWJ77_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9454031.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2348 a         1079 c         1319 g         1994 t
ORIGIN      
        1 gctcattttg aaaatttaaa catactcgtt cttatccaaa tatataaaaa tcgcccctca
       61 ttcatcgaga ggcgataagt tctaatttaa ctcaattttt tttgtttctt ttggctgata
      121 agctgtttta ggtaatacca atttcagcac accgttttta aaggttccag tgattttttc
      181 ttcatcaaca ttaggaagat agaatgaacg tttgtaagag accgaatgtc tttctttccg
      241 tacatagttg ccatcttctt tatcttctgt atgggactca tgattggcgg agatcgtcaa
      301 agtattatct gaatattcaa ctgtcgtgtc ttctttgtca cagccaggca tatcagcagt
      361 tagtttgtat tctttttcat tttcgactaa atctactttt ggataacttg aaacacctaa
      421 aaaatcattg aatgctggac taaacacgtc attgaaatca gggaacatgt cgcgaatgct
      481 aggtacattt gccatttttg atcatccttt caataagtat tttttattta tccctattta
      541 cgtaataact ctattttgaa aaaaatgcaa gtataatggt tagctttgtg aatatttcat
      601 ctaatacgat acaaaggcta tctttctaat ttgaaaaagg gatcattaaa ataataagaa
      661 gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc aaatatagaa aaattgaatc
      721 tagaaaaccg cccgacaata aatttcatta actcagttga ccgaaaatga agtagatacc
      781 aaaaatttat caagagagca agtagagaag tgagtatatt cataagagaa atagctgtaa
      841 cgaattatta ggttaatgca aatgcttatt tatactccgt ataactttct cagaaaaaat
      901 gataccaata atgacttgct agtactgtaa atccgtataa tatcatctaa tcaaaatggt
      961 gttccatttg catctgtcat cgtaatcgat ccttcgggtg acactcgata aaaaccaacg
     1021 cttccagatc ctccttggga acggatttgt ttgctggaag ctgtgaacaa ataatctcga
     1081 tcaatttttt gataaaagct aataacaata tcgtcacttt gtaattcgga acggtgggca
     1141 ataagaaata gaagtgcttt gtcagagttt tctaatggaa gagaaggtac agtgctttct
     1201 tgcatttcag atgttgaaga aatgctaaac gactgaatac ttgattggct atgttgcgaa
     1261 tctacattac cagtagaagt attagtaatg gaagtagtag aagtactttt agttgaacca
     1321 acctcagttg ttgaaatggc tgctgaagat tctttaacag aattctttgt tttatatcca
     1381 caggcagaaa aaagtattac agttcccaac aagcctgcgt aaatcccttt atgcatgata
     1441 gctcctccta aaatagatta tctttattca gtatatcact taaacaaaga aaagcagatg
     1501 gtttaaaatg aaaaaacctt cccaataaag ggaaggttca tgaagataat aactttttta
     1561 ataattatag ctttctaata tctttcatca acttttctag taatcattgg gaaaaatttc
     1621 aagcagtaga tcgcacaaat acctacgacg atataaatga tcttcgcaaa aatagttgtt
     1681 gaaccaccag caatcatagc tactaaatca agttcgaaca agcctaccaa cagccagttc
     1741 aaccctccca caatcaataa agttaatgca atagcatcta aagttttcat tagaattctc
     1801 ctttcttaaa tgataaaaca attatatatc aaaaagagaa acattgctta tataacgctc
     1861 aggaatttta gcaatctatg caaaaagatg aaaatttttt ttgctcttcc attagtacac
     1921 attttttagc tgaagctgta aatttcatgt cttaattttt tttagtgagc ttttatactg
     1981 gatttgtaag aaaaaagaag gagaggatca catatgtcgg attggttgga tattgcagga
     2041 aaagtagtaa ttgtaactgg aggttcctca ggaattggaa gaagtatcgt cgaaaatttg
     2101 ttgaagcaga atgcacaagt agcgaatttt gatgtaactg aatgccgtat acagcatgaa
     2161 aacctactat ctttaaaagt cgatgtgtct tctaaaacgg acataaaaga agggatatat
     2221 aaagtaatga aacattttaa gacgatcgat ggtctagtca ataatgcagg aataaatatt
     2281 ccttctcttc taatagatag aaatcatcca aaaagcaagt atgaattatc agaacaagtt
     2341 tttgataaga tgatcgctgt caatcaaaaa agtgtatatt tgatgagcca agcagttgga
     2401 agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc tttcttctga atcaggattg
     2461 gaaggatcag aaggacagag ctgttatgct gctacaaaag cagcgatgaa cagttttact
     2521 cgttcttggg caaaagagtt aggaaagagg aatgttcgtg tggtaggtgt tgcaccaggg
     2581 atactggaag aaaccggttt gagaacacaa gaatatgaag aagctctttc ttatacgaga
     2641 ggtataagcg tcgaacagct gagaaacggt tacagtcgaa caagtacgat ccctctagga
     2701 aggagtggta agctgcaaga agtggcggat ctagtctgct attatctttc agaaagatca
     2761 agttatatca ctggagtcac gactaatatt tcaggaggta aaacaagagg ataaaggata
     2821 ggggggacgc acgtgacgct agtaaacaga tggtaccaat tattgaaatt actagtcact
     2881 cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca aaaccagtaa tcaaaccata
     2941 aaaaaagata ttggggagtt gaatgaagaa attgccggta ttgcaaaaat catccaaaaa
     3001 aacaaacatt ttgaacttga aatttatgat tttgattttt ttgatgagat catgcaagga
     3061 aaattgaaaa cagccagtga tttcaattcg gcatccaaaa gaatcgctta tttactaaat
     3121 aggctgatcg aaacagagga tttcttacgt atggatgatt tgtctgaaga agtcggtgtc
     3181 agcagaggaa cagtggtcaa ggatctaaag acattaaaag agatgatcaa tgattttgat
     3241 gtaaagatta caggcacacc aaataaagga ctaagaataa taggagatga gctagaatta
     3301 cgactacttt tattttattt tgtttctccc tacttttcag agacgaaaac agaacagttt
     3361 ctagaagaaa aacaagcaat caaacatttt cagaagaaga cgaatgctag taaacaagaa
     3421 atcacccttt taacgaaagt tatcggtatt tctttgaata gaatctctag taactatctg
     3481 ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat atatccaaga attcaatgaa
     3541 ctgatcaatc agttggaaga aatctatgaa ctaactttga gccaatatga aaaagatttt
     3601 atcagttttc ctttgaacat catcaacaca agtataacga tgataaggaa agctaactct
     3661 gatttgcgct tatatttcga ccccatgatg atgcgtattc gacgattaga tctcattgat
     3721 ctggatgagt ccttcttata tcaagagatg aaacagcacc tctctttatt gataaatcgg
     3781 gtaatttttc gttatcgatt gactgattta ttttatggtg agatagaaaa aaagtatcct
     3841 ttttcttacc aaatcgctag cgaatgcata caagcattag aagctatctt gcaacataag
     3901 atctctaaag tagagatcag ctacctaacg ttgtatttgg agttagcatt tcgatatcaa
     3961 aaagagcatt tttcctctaa aaaagtagcc attgttcata cgacaggaaa aggaacagca
     4021 ttaatgatcc aaagacaaat caaaaggata ttggggaggg atatacaaat aacacatgtt
     4081 tcagaagaac aatatgaaaa agtagatcta aacaaatatt tcgctgtttt tacaacgatt
     4141 ccgttaaaaa atgtaaatga agagacaccg gtgatccatt tgactaatct ttttgatgac
     4201 tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc gattagccgg gtttcattct
     4261 actgtttttt tctatcaaat atggaaagaa aaaaagacgt atcaagaata tttaaacaaa
     4321 ttagtcgctc ctttgatcag aggctattat gtggatgaag aatttgaaaa aaggatcttg
     4381 aaaagagaat atcagcaaag cacatttgtt aacaaagaat ttgcttttcc tcacacaacc
     4441 aaccagctat ctcaacacat tgttttatca gtagggctaa taactgatca aggtttagca
     4501 gaaacaggag gaaatggagt tattttttta tttgccatac ctgaagaaat gacggctgaa
     4561 gttgagaatg aattatttga gctatacgat atcctatttg aactcataaa aatgggaata
     4621 gatgagttta ttgaattagc accgacacaa gaatcatttt taaaactaat caaaagtaaa
     4681 ggagagagac ttcatgaatg atatgtatct attcatagtt ttcgcagctt cagcttatgt
     4741 gctgcaagca gttatcagtt ggaagcaact aaagcatttc aatcaaacat atagcgaact
     4801 gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg aaaatccgtt caggcacgat
     4861 tgttatgcta gctatagacg atcatgatca agttttagaa gtcagaaaaa tgcaaggggt
     4921 tacatctttg gcaaaatttc attcattacc tgtctatgcg ggtgaagata tccattacat
     4981 cgatcgttac catccctcag ttcgagcgga agatcgtttg acacaagcag ctttagaaaa
     5041 tgccagagaa gtatacttac gaatagaggc aggaaatgaa aaaagtgaac aaccttcatt
     5101 gccttttatt gctatgctca ataattttcg gatatggaaa agtacgatca aagaaaaaag
     5161 gagtgacaaa cgatggatat cataacaaaa tttgctgagg gatttatgag gctgttccaa
     5221 acaggtgcag aaacattcat aagctggatg acaggaatcg taccagttgt cttgatgctg
     5281 atggttgcaa tgaatacgct gattgctttt ttaggtgaag accgagtaac gaaagtagcc
     5341 aaattatcag cgaaaaatcc gcttactcgt taccttgtgt taccgttctt atctgctttt
     5401 atgttgggaa atcctatgtc atttacaatg gctcgtttcc ttccagaata ctacaaacca
     5461 agctattatg ctgctcaagc tcaattttgt catacgagca atggtgtatt cccccatatt
     5521 aatcctggag aattgtttgt atggatgggg attgctcaag ggatcgaaac cttagggcta
     5581 aattcgatgg agctggctat ccgatacttg cttgtgggac tgctgatgaa ctttattggt
     5641 ggttggataa ctgattttac tactggattt gtatgcagac agcaaggaat cgttcttagc
     5701 aaaaaagtag agctttcagt tgattagaga aaggagagtt ttaaatgtat ggtgatatta
     5761 agatcgtaag aggaaacggc gggtatggag gtcctttgat tattaggccg acaaaagata
     5821 aacacaagtt catctatgtc acaggtggag gtgagaggcc aatgatcgta gataaaatag
     5881 aagaactgac aggtatggag tctatcaatg ggttcaaaac atcaatacct gatagtgaaa
     5941 tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg tggaatttat cctaaaaaag
     6001 ggataccaac catcaatatc gttcccacag gaaaaagtgg cccattagca caatatatca
     6061 ctgaagatat ttatgtttca gcagtagggt tagaacaaat acagccacta atggaaactg
     6121 atgataacaa agaaacagta aatactgcat ctccagataa aaagtacaaa tatgatactt
     6181 ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg gatagcaaaa attggaatgg
     6241 gcgcgggaaa agtagttgct acattcaatc aatcagcaag agaagcagta gatacgatga
     6301 tccggacagt gatccctttt atggctttcg tttccctttt gattggtgtg atacaaggtt
     6361 ctgggatcgg taatgtattt gctaacatta tggcaccttt agcaggaaat gcagttggat
     6421 taacaatcat tggctttatt tgttcactgc catttttatc tcctttgctt ggtccagggg
     6481 cagttattag ccaagtgatt ggaacattga tcggtgtaga gattgggaaa ggaaatattc
     6541 ctccccaatt agcattaccg gcactatttg ctatcaatac tcaaaatgca tgtgatttta
     6601 ttccagtcgg tctaggtttg gaagaagcaa aagctgagac agtagaagta ggtgtaccat
     6661 cagttcttta ttcaagattt cttaacggca tcccacgagt tatcattgct tgggtagcaa
     6721 gttttggatt atatagctga
//
LOCUS       DACDRK010000098         6672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDRK010000098 DACDRK010000000
VERSION     DACDRK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.099"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(4..1029)
                     /locus_tag="IWJ77_002477"
     CDS             complement(4..1029)
                     /locus_tag="IWJ77_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9454032.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(1274..2545)
                     /locus_tag="IWJ77_002478"
     CDS             complement(1274..2545)
                     /locus_tag="IWJ77_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9454033.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2829..3776)
                     /locus_tag="IWJ77_002479"
                     /pseudo
     CDS             complement(2829..3776)
                     /locus_tag="IWJ77_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3788..4261
                     /locus_tag="IWJ77_002480"
     CDS             3788..4261
                     /locus_tag="IWJ77_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9454034.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(4434..4988)
                     /locus_tag="IWJ77_002481"
     CDS             complement(4434..4988)
                     /locus_tag="IWJ77_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9454035.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(5461..6630)
                     /locus_tag="IWJ77_002482"
     CDS             complement(5461..6630)
                     /locus_tag="IWJ77_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP9454036.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT         2134 a         1217 c          978 g         2343 t
ORIGIN      
        1 tttttaatcg atattttttt cactttctct aacaataata ttattagaaa caatcacttt
       61 gatcgaaaca tttctaactt cttttattcg ttcattaacc atgcgaactg ctagttttcc
      121 aatttcttct gtattcacat tcaccgtatt taatgcaggt gttaaaaatt cagcaacttc
      181 aatattatca aaactcacaa tcgaaatatc ttctggaata tgtagaccat ttttttgaag
      241 tgaacgataa acccccacag caataggatc atttcctacc atgatagcag tcggcaactt
      301 atctttagca tgttttttca aaagtaaatc tgctgcctgc attccgtcaa gtgtggtcca
      361 gccttctaaa taaatttgac tatatttttc taatcctttt tgtttcatcc acgtctcata
      421 ggctattttt cgataatcat catctgacac gtgtttattt ccttggctat ctaaacgaac
      481 acgtcctcct ccgataaacg caatgtttcg gtgtcctttt ttatacaatc gatctaaatg
      541 ctgattcgtt gcttttgtta aatcagtata aactgcatct acttcagctt caatactagg
      601 gtcatcaatc actacgacat tcgaattaat tgctgatact tcttcaatca cagaagatag
      661 cacctgccca ataatcaaca caccttgaca ttttagcaaa tcgtcttgct ccaacggtgt
      721 ttctgacaaa cgaataattt tcttcaaatt gactttgctt tgttctgctt cgacttgaat
      781 ccctctgcga atcgttcgaa aatagggatc ttccaactca tctacttcag aaactgtcgt
      841 tattaatgca atatttgtta aattttttgc aacgggactt tttttgacat aatgataaaa
      901 attaaccgat tctgcaatct ttcgtttggt ttcatcggtt actgaaagtg tcggatcctc
      961 atttagcacg cgcgatactg ttgcaatgga cacacccgcc cgttttgcaa catccctaat
     1021 tcctaccata atagctcctc ttttcatatt tgaaagtgtt tactaacata taatcataca
     1081 ttttgaaaat aagcaaattt cgttaaaaat aggcactgta atcataatta aaaacaagaa
     1141 aaaatatccg aacaatttag agtaaataat gctaattcta gtgaatttgc gacgaggaac
     1201 tgctagatca cagttcattc tctctatcgt tcggatattt tattgttaac tatacttatt
     1261 tcacagagct tttttatttg attttgtgaa cagagtctcg agaaatcaat tctccctcta
     1321 atagtagtac ttgatttttt atttcttctt gtttggccag tccaatcagt tggtgaaaag
     1381 caatcgcccc cttgttacct ggatttagtc gtattgttgt caacgcagga tccattaatt
     1441 ctgcaaaagt aatatcatca aatccaataa tcgataattg ttccatcact cgtttattct
     1501 tattttttaa aaattttact aatttagccg ctaaataatc tccagtaaca acaattgcag
     1561 taaattcagc taattcttga taaattcgct caaattcaga gttgttatat atccaatgcg
     1621 atttaaaggt taaatgatat ttctttaatg tatcgatata agcttgatgt cgttcttgaa
     1681 taacacttgg ttcacctaca atgtagtcaa aagaaagaaa cgctattttc tcatgaccat
     1741 aatgaatcaa atgttccact gccaatgtac tagcttggta atcatttgta tttacaaata
     1801 aacgattcat ttgttgttca agttctctta atgtctcttt tgaaagatgc gtatcaatat
     1861 aaacaacagg accatcaaaa acgttattta attccaaaaa ttcttttaca cgtacaccaa
     1921 caacaatgaa cccctcaaaa cgccaatttc tctgaatagt tttaacatct tctgctgaat
     1981 taacattatg caccaataca aaataaccat gattctttgc ctgttcctca attccagaca
     2041 aaacttctgc ggtaaatgga tcagaaaata caaaatcagc attatcaata gccgaaaaat
     2101 aaagcaatcc tattaaaaat gcaatcgtct taccgattcc ctgacagaca gaacacattc
     2161 cactaggatc attaaatgaa taagcatttg catatcctaa actaggtgtt gcaaaacatg
     2221 aaaacaacac acgaaataac gcattgatgt tagtaatcgt ccctaacgtt gagcgcgcac
     2281 tgccaccaat cttcttttga ttgaccacaa tcgccgtaga aagattatca atctcatcaa
     2341 cctctggacg actgtattta ggtaaaaata aacgggtaaa actatcatag gtgtcattca
     2401 gttgacgacc agcttcttgg gtaatagtat caaaaacaat cgatgatttt cctgaaccag
     2461 atacgctggt aaaaatacta attttatttt tgggaatttt gagactgaca tttttaagtt
     2521 gtttctcttg cgtggataat ttcaatatac tcgttcattt ggtactcccc tttattttga
     2581 aatagttgag aatgtttcaa tgagacacgg tatttttggc agatgtttag tttacctaga
     2641 aagaggtata aaaatcaaga aaaaaagaga ggctgactca taagtagttt tattagtcga
     2701 aaatgatagt ttataaaaat aggaaccgca tcaaatcaac attctttaga atggatttga
     2761 tgcgtttttc tttatttttt gacttatgag tcaacctctt actcttcgtc atggatttga
     2821 ttagctaatt attgctccac ttgttcacga atgaaattta agtactgcgg taaaaataaa
     2881 ggaatttctt caaagcgtgg actttgattt tcatacgctt ttgcatctag gtttccttcc
     2941 cctgtagcaa tttcaatcaa taattgattt gggtcacgat aataaagtga tacaaaaaat
     3001 tcacgatctt ttattcctga atttataaaa ttgcgctctt gtaattgttt gtcgatgcgt
     3061 tgtaaatcag atttattttc tacgccaaac gcaacatgat ggacgctacc aatcccttca
     3121 atggcaactg gattctcacg atcttgaata atctgaactt cttgatagaa ctgagtgtct
     3181 tgattttcta agattgtcac tcgttgatga cccgaaaaga aactgacgta ttctttgtgc
     3241 taccagccta ccgacaataa ctctcgttca gtcgcatcgg aatattgtac gcgtagttga
     3301 atggcatcaa ttcctaaaat ggcatgctct gtagggatct ggtctgtgac atacgggaaa
     3361 taatcatcaa tatcttcaaa ttcgcgtaag ggaactaaag ctaatcgtgt tgcatctggc
     3421 ccttcaaaac gtaagatagg tcgtccagaa aatgattcga ttccataatg gcaaacgcct
     3481 tgtgtttcaa aatatatttc ccaaaaatgc aatgatgctt cggaaggaac ttttagtatc
     3541 gttcgttcaa tcgtattggt tccaaaaaac tggttgttcc cttcttgtac ttcaaaaaaa
     3601 gtcagttcag ttcccacacg gtggtgatta tctgaaaaaa acaggtgata catggtatga
     3661 tcgtcttgat taattgtttt cattagtaat tttaaaccga gtatattatg ataaaagtca
     3721 aatgtcggtc gaataaagcg attcacaata gaaatgtgat gaatacgtgc gggcataata
     3781 tctaaaaatg agaatcaaac ctataaaaga tggtgaacaa cttttgtttt catcagaaga
     3841 aaataagccc ctttatttag actatgtaaa tcacaatttg aaaatcatca ttaaagagaa
     3901 taacttaaaa catatcacac ttcatggttt tcgtcatacc cattgtagtc tgctctttga
     3961 atctggtgcg tctgtcaaag aagtgtatgt tcgacttgga catacagata ttaaaaccac
     4021 tatggatgat atatacaccc atatcgcgaa aaagcagaca gaggaaacgg caattcgttt
     4081 tgcagatttc atgagttcta cagaggaaaa gcaaggaagt ttgcagaagg tcaaaagtat
     4141 ggtcaaaaac gaaaaaagca gactatccct attagatagt ctgctaaacg ttgagttatc
     4201 agtatttttc ttattccgca gcgtttgcgt ttttgagacc gtattgattc tcttttttta
     4261 atttcatcct tttccaacct aaaaatgtta tttaattact tagtttatat aacacgaacc
     4321 tataccaagt accagtaacc ccagctttta ttccgcgaac tttatgaaat aaaatcgttc
     4381 tagataaaaa tagcttttaa tctttgtctt tactgtttat aaaaattttt aaattataca
     4441 cctagtttaa atccacgaag tgatgctcct atttcatctt cagttatacc aatgtacctt
     4501 tttgtaattg cttgcgagct atgattaaaa ataaacatta aggtagcaat gtctctagtt
     4561 ttcttgtagt agtgataccc aaaagttttt cttaatgtat gtgttcctat atcatctcgt
     4621 cctaattttt ctgctatatc tgcatatatc tgatagacac gatcccttgt tatatgtcga
     4681 ccttgttgcc gactcggaaa taaccaatca ttttcttctt taccttcagt atataataaa
     4741 atttcttctc tcaaattatc tataaaaatc ggttttcgct tacctgtttt atgttcaaca
     4801 ataataggct tacttttgcc tcgtatatct ccaactttta atcctataat atctgacata
     4861 cgtaacccat tattgattcc aaacaaaaat aaaaaactat ctctttccgg attacttgtt
     4921 cttcttagcc aaaactgaaa atcttgtatc tcttcatttg tccttaacgg ttgtacatta
     4981 tagctcacta ttgtttccct tcttaaaaat attctgagtt aatgattatg tatatatttt
     5041 accataatca ttaacttata tatataaact tttgaaagtc aataaaatca agggatttag
     5101 tttatgattt tatatcattt cagtaactca gaatattccg acttttttgt acaaaaaaat
     5161 taaccttact tatatgaaga ttctaagctc ttatttaaat tttagacggg cagttatccg
     5221 tagaagttct tagaatagct aatttacata attaaattat atgaaattta agtttgaaca
     5281 atatactaag aactcccctt actataaagt gaaataaatt agcatatgaa taaccgtgct
     5341 accaagcttt tcattaataa aatattttta ttaatatttt ataactttac gcaagatcat
     5401 aattttgtcg ggagcgtcaa taattttgtg taaatggaaa agcccattct gttgatcaag
     5461 ttaaaacatt gattctaatg tatcatgtat ttctttaaaa cctttatgaa ctcggcatag
     5521 aaacttttga ttatagttgt tgaattgaga tactagaaat ctctccagag attcttcatt
     5581 aggaaattgc tcttttcttt tggtatattt ctttaattgt ttgttaaaac cttcaatcaa
     5641 attagttgaa taaatagttc ttctgattga cggagggaaa ttataaaagg ttaatatagc
     5701 aggattcatg agcaatttaa ccactcgtgg atactgcttt ttccatttat ctatcataaa
     5761 ccttatttga ttatttgctt cttcttttga acttgcttgg taaactaatt tgaaatcgct
     5821 acagatttct tgtcgatcac taacacgaac cttatgagcg atatttctag atatatggac
     5881 acaacattgt tgaaattgag catttggata aacactatga atactatcgt caataccact
     5941 taaaccatct gttacgacta acaaaacctc ttctaaacca cggtctttta gatcttgaag
     6001 aatctctttc caaacataag cagattcagt tggagctata gtaaatccta gaacctcttt
     6061 agttccatcc aacctgatac caattactat atagacagct tcttttgata cggtttgtct
     6121 ttttaaggga atatgagtag catccataaa aatgactgag tatttagctt ctaaagttct
     6181 ttctttaaat gccgaaacat cttcagacac aattttactc atatttgaaa tagtttgtgg
     6241 tttgtaataa tgaccataca ttttttcgat tagatcagag atttcggaca tagtaattcc
     6301 tttttgaact agctgaataa tagtggtttc taaagagtca ttggttcttt tataagcagg
     6361 taaagtttgt tgagaaaagt ctccattcct atctctaggt attactaaat tcaattcacc
     6421 gtactctgtt ttaaaagagc gcgaataatt cccattacgg gaattacctg aattaaatcc
     6481 agctctatca tacttttcat agtcaagaaa taccgttaat tcagcttgta ataaagcgtt
     6541 aatagccaat tctaggtgac gacgaaacaa atcatccaaa tcacctttat tgattagtat
     6601 ttccataatt tctgtagtaa aatcgttcat atggaagtcc tcttttctgt gaatgtgttg
     6661 tggtaactct at
//
LOCUS       DACDRK010000099         6645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDRK010000099 DACDRK010000000
VERSION     DACDRK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.100"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..444
                     /locus_tag="IWJ77_002483"
     CDS             <1..444
                     /locus_tag="IWJ77_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP9454037.1"
                     /translation="NYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKAN
                     IFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDL
                     SMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            725..1945
                     /locus_tag="IWJ77_002484"
     CDS             725..1945
                     /locus_tag="IWJ77_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9454038.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1942..4662
                     /locus_tag="IWJ77_002485"
     CDS             1942..4662
                     /locus_tag="IWJ77_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9454039.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4646..5590
                     /locus_tag="IWJ77_002486"
     CDS             4646..5590
                     /locus_tag="IWJ77_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9454040.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5659..>6645)
                     /locus_tag="IWJ77_002487"
     CDS             complement(5659..>6645)
                     /locus_tag="IWJ77_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9454041.1"
                     /translation="AATSALAVLTLAACSGDTNKDIATMKGGTITVSDFYDEAKLESS
                     NQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAGYTKDTYKE
                     YIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDVKKSADDGD
                     DFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDVITTTNPTS
                     YATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEELKDANVKIK
                     DDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDSSK"
BASE COUNT         2341 a         1082 c         1277 g         1945 t
ORIGIN      
        1 aactatgagc ttgagtatga aaaatctatt tgttcaatcc cttataataa tattgaggaa
       61 attatagaaa aggatgctat cagcatttac aaatttttta tttttattcc taatcgtcaa
      121 ttgaaagcta atatatttaa ccatttgaaa aagatgctaa atgtgtctgt tactgaatcg
      181 gcacctgtaa atattgaaat cgtacctcaa aatgtatcta aaggtgtggt gtttgatcgt
      241 ttggaatcaa tctacaattt aaagaaacct ttgagaatcg cgatcggaga tagtttaaat
      301 gacttatcta tgtttgaaag tgcagatatt agttttgcaa tgggaaatag tcatcaagaa
      361 atcatgaaaa aagctgatta tgtcactgtt tcaaatgata aagacggagt atctcaagca
      421 tttgaaagaa ttcttaccat ataaactaat taaggtagta tagaacatag aattctaaat
      481 acggaaaaat ggagcataaa ttccagtaca aatagtactt cttcagtgtc aaacgaagcg
      541 aatataacat agatatttag gattgtgaca aaaatcatgt cacaatcttt ttttcgaata
      601 aacttatttt ttgaagtcgg ccatttatcg taatttcttt gcataatacg aacaaatttt
      661 cggttattga tgaaaaaaaa cattgaaaat ggtatgataa aagaaaaaaa ggaggaaatt
      721 tttaatggtg aaatttatcc atgcggcaga tttacatatg gatcggtcat ttgaaggact
      781 gacaactctt gataaaactg tacaggaaaa actattgaag acgaatctga ccgttttatc
      841 aaatatcatt gaacaggcaa taataaataa cgttgatttt gttttacttg caggagataa
      901 ttttcatcaa aatcgtcctt cattaaaaat acagaaacat ttttcagaac agatgaaacg
      961 gttagaaaag aaccatatac ctgtatttat aatattcgga aatcatgatt tctatcaaaa
     1021 agagcggtat tggttctcct ttcctaaaaa tgtccattta tttacatccg aaacggtaga
     1081 gacaaaaaaa ataacgataa aaagtggaga aactgtctct ctcagtggat ttagttatcg
     1141 tcaaccgtgg attcaaaaag acaaagtgat ggaatttcct tctagagaac tcacagatta
     1201 ccatattggg ttgtaccacg gggaacctgg cgtctctcaa aaaggaaact atgctccttt
     1261 ccaaccctca aagatgcaag aaaagggata cgattattgg gctttagggc atatccatgt
     1321 gccaacggtc ttaaatgaaa agcaaacaat tgtttatcca ggtgcgcctc aaggtcatac
     1381 taaaaaggaa caagcttcta catctattct actagttgaa ttaagtaaag gaagttgcca
     1441 aatacatccg attaaagtag cagaggtcta ttggaagaca aaggaaatat cattgaggaa
     1501 agcaagaacg acaaaagagg tcattgttca cattcgtcag cagctttcaa aagtagagga
     1561 tggatttagt ttaattgaga taaaattaaa agattatgac catctcaata cagatgtttt
     1621 ggaacggata caatcaggag agctattaga ctatttgtta gaggaattct cagataggat
     1681 aaatgatttc tttatttggc gtatttctct catagagaat actttttata cgaagacccc
     1741 acttgctgct tctgctaaat tgatggaaca gttatttcaa tactatcaga cacctcaaga
     1801 tttccaacaa attttaaatg aagtatatag tcatcaagaa gcggctagaa tattgagcga
     1861 actgccggaa tatcaagaag aaacacttga aaaagccaag cagctgctta atcaagattt
     1921 tttatttgag gaggatcagg aatgaagatt ttaaggatcg agattgctgc ttttggaaaa
     1981 tggcgtcaaa aaagttttga cttttattca ggcaatcagc tgatctatgg gggaaacgaa
     2041 gcaggaaaat caacgattta tcaatttatt caagcaatat tatttggttt cccttcaaaa
     2101 ggccgtaaaa aaaaggatta tacaccaaaa gacggctcag cctatggcgg caaaatatgg
     2161 cttaaacatc ctgtatatgg tgaatttgct gtcgagcgct acaagcagca aaatcgcgga
     2221 aaatcgaagg tgtggctggg ggatcaagtg ggaagtgatg agttactaga aaaactgatc
     2281 gcacctttga cgaaagagtt attccagcag gtatttactt tccagcaaga gcagttgatg
     2341 gaactagatc atttgagaga aaaagagcta catgatgcgc tgatttcttt aggcatcacg
     2401 ggcagttctc aagtattcca aaagcggcaa gaatattatc agaaagcaca acaattattc
     2461 aagaaaaaag gccaaaaatt actaatcaat caaaaattaa atgaatggaa agagctgcaa
     2521 gaaaaaattc atcaaaaaca agaacaggaa gcttattttg gcaagttgat ccaacaagaa
     2581 caggaatacg agtcgcaaca gcagcagtta ttggcacaat taaaagaggc gaacaaacaa
     2641 ttttcattag ccagacagca agaacttaat ttttctttat atgaagaatg gcaatctctc
     2701 aaaaaaaaca gcactacagg tcttcctgat gagcaacttc ttttagatct ggagtctttc
     2761 tccaaacggt atcagcaaac aaatgagcgt ttggaagaat tagaagcaac actggaaaag
     2821 catagcggga tggatcagca gtcagccaga tattattttt atttagagca ggagcaggaa
     2881 ctaaaagatc tccagcaact tcaatttaca gcggaacaat taaacgaaga atggcaaaga
     2941 ttacaaattg agactagaga aacaaattca gaattgaaga ttttggaaca aaggtggcat
     3001 tggcagaaaa atccgttacc ggaagaattt ttatatgaag aagaatggaa gcatcttctg
     3061 gctgaagaaa gaaggattgc tgatctagaa agccagattt ctgtgcgtct ccaaatagct
     3121 aaagaacagc aagagatgat tgaagcagaa gtgaatgact tggaagagac ctatccagca
     3181 ttattacaaa atcagtcaga gatagcacca gtctcttcat ctaaaccgta tatgctgatg
     3241 gctggcggag caataatcat tgcaggtata tttgtaccaa tgcccttgaa agtgatcctt
     3301 ttactaattg gtgcaggcat gattggctat ttcttctaca aaaggcaacc aaaaattaaa
     3361 actaaaaatg atttctctga gataaaagga atgtggcaag aaaaactagg acaacttgat
     3421 ctagccttag ctcaattagc tgaagcagaa gaaaaacaaa agagaactgg agaagaaaaa
     3481 cagattcttc agcaagaagt atatcgtttt gcggatcatc ggcacttagg aaagatggat
     3541 acgctcaaac tcatgaaaga atatggacag gaagtcatag attatcagaa attatatggc
     3601 gtatttgagc ggaaacataa aagagaacag gaaatacaag aaaaactacg acaaatcgac
     3661 caaaaatttg agtttgttaa agaatggtta ccgatacaag ataagacaat ttcagaaaaa
     3721 atggtattgc tttcccgttt tatacaagag atggaacaga tgaaatttgc cagaagctat
     3781 cagcaaaata ctttattgaa gcaagaaatc aatcaattga aaaagaaaca aaaagtatgg
     3841 atacaagaat acagcgaaca attgactcaa gcaggcttat cttatccttc ggaagtacct
     3901 gtctttttac aaaaagccaa acaagccgaa cagcagatga aaagatacaa tgaattatcg
     3961 gaaatgctta ctccactatt tcctgaagag ctgacaaaag agaaattatc tgtaagactg
     4021 cgccaactga aagaaaagca atcccaactc cagcaagaag aaaaagaagc aagtgaaaat
     4081 cggcaacgac tccaacttca agtagaagag ttgcaaagag acggcacatt ggacgaactt
     4141 tatcaacaag ctagccggca aaaagcaaaa ttacaggaat tactggagga atggggagca
     4201 cttgaaatcg ccgggacatt cttaggtgat ctttcaacag agatgagtga aagacaatta
     4261 ccccagttgt taagagcagc aggtgattat ttaggtattt taacaaaagg aaattatcag
     4321 aagatattac tgaaaaatga tacactgact ttaacagacg gatatacaga atttcccatc
     4381 tatgagttat ctacaggtac gaaagatcag ctgattatgg cgatgagatt tgcttacctc
     4441 tcattagaag ctaaccgttc gatatgcccg atcatcattg atgatggctg gcttcattat
     4501 gatcatcagc gaaagtatca attagctgaa cttttagcta aatttggaga aaagtatcaa
     4561 ataatttgtt tttcttctga taaagaaatg gtaagctatt accatgagat gaaccaaatc
     4621 gtaaaacaat tagaaggagc aaataatgaa aaaaattcgt gatttagtaa tcgatgagca
     4681 gtttgaacaa tttgtcctga tcaaaagtgc agatgtaaga atcgctaaaa acggaaaaaa
     4741 atttattgct tttactttcc aagatacttc aggtactatc gatggtaaat actgggatgc
     4801 ttcggaggag gaaatcaata aatttacagc cggcacagtc gtttttctaa acggtaaaag
     4861 agaggtttat caaggaaacc ctcaagtaaa gattcttcat atgcgcttaa caagggtaga
     4921 tgagccagga gagcctagtt tatacatgga aagagcccct ttgaagaaag aagaaatgga
     4981 agaagaaatc aatcaattgt tgtttgagat caccaatgcg cattggaatc gtattgttcg
     5041 ctatctactt aataaatatc aaaaacaatt ttttgagttt ccggcagcaa aaagaaacca
     5101 tcatgcattt gccagtggat tagcttatca tactttgaca atgcttcact tagggaaatc
     5161 aatcgccgat gaatatcctg agctgaatcg agcattgttg tatgctggta tcattttgca
     5221 tgatttagga aaggtgattg aactttccgg tcctatgtcg acagaatata cgcttgcagg
     5281 aaatttgatc ggtcatctgg tattgattga cgaagaaata actaaagcct gtcttgcgtt
     5341 gaaaatcagc gaagctgacg aagatgttct tgtattacgt catatggttt tagcacacca
     5401 tggcttatta gaatatggct cacctgtacg tccaagaata atggaagcag aaatattaca
     5461 tcaaatcgac aatatcgatg cttcgatgca gatgatgttg acttcgattc gtcaaacaga
     5521 accaggaaaa tacaccgatc ggattttcgg tatggacaac cgaagtttct atgtaccaaa
     5581 agatttatag caaaaattta tttttttcat tcaaaacaaa aaaagcgaga cataagtctt
     5641 cgcttttttt gttttctttt attttgatga atcagttgtt tcttcagttg aagattcttt
     5701 tgttgaatca gttgattttg tgtcagaact ctttgttgaa gctgtagttt cacttgagtc
     5761 tttcgtactt gatgaagttg ttgtgaatgc actcaacaca ttttcaaagg catcatcttt
     5821 gatttttaca tttgcatctt ttaattcttc accgattact ttagttgtga atgcattgtc
     5881 acttagttta gtttcagtag cgatatcttt tagttgatct ttgtatttat ccatgtcatt
     5941 gcctttattt tggtttttca ccattttgac aacgtagtat tctgtcgcat aagaagtagg
     6001 atttgttgta gtaataacat cggagatctc accgtctttc aatttgaaag ctgcttcttt
     6061 tacttctgca ggaatagttg ttgtcgttga gtcgaattta actttaccgc catcttcttt
     6121 tgtctctgta tcagttgatt tgtctttggc taatttagag aaatcatctc catcatccgc
     6181 tgattttttc acatcttttg cttcatcttc acttgataat ttgatgattt gtgcttctac
     6241 ttctggatgg aaagatttcc aagcagtttt caaatcgtca tctgtaatat ctacgtgagc
     6301 tttcaagcca gcttcaaaag ctaaattgtt acgaatatat tctttataag tatctttcgt
     6361 atatccagca gcttctaatt gtgattcaaa agtatcacct aatgatttcg cttgtttgtc
     6421 gtattctgca tccacttgct tatcggttac tttatcgcca tatttgttat tgaatacttt
     6481 ataaatgatc atccgttgta ctaaggattg gttcgatgat tcaagttttg cttcatcata
     6541 gaaatcagat acagtgatcg ttccgccttt catcgttgcg atatctttat tagtatcgcc
     6601 tgaacaagct gctagagtta aaacagccaa cgcactagta gctgc
//
LOCUS       DACDRK010000100         6643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDRK010000100 DACDRK010000000
VERSION     DACDRK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.101"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IWJ77_002488"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IWJ77_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IWJ77_002489"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IWJ77_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9454042.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IWJ77_002490"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IWJ77_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IWJ77_002491"
     CDS             complement(2143..2373)
                     /locus_tag="IWJ77_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9454043.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IWJ77_002492"
     CDS             complement(2603..3421)
                     /locus_tag="IWJ77_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9454044.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IWJ77_002493"
     CDS             3582..4271
                     /locus_tag="IWJ77_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9454045.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IWJ77_002494"
     CDS             4285..5787
                     /locus_tag="IWJ77_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9454046.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IWJ77_002495"
     CDS             5800..6276
                     /locus_tag="IWJ77_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9454047.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IWJ77_002496"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IWJ77_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1033 c         1204 g         2234 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACDRK010000101         6413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDRK010000101 DACDRK010000000
VERSION     DACDRK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.102"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..2604
                     /locus_tag="IWJ77_002497"
     CDS             <1..2604
                     /locus_tag="IWJ77_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP9454048.1"
                     /translation="LRTFSTLNKKTVSHIFSTADSQAIINYLEKQKLSEGTVNITYNP
                     IKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERYLKE
                     KRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDTSVY
                     NPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMMSTS
                     RVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNNS
                     NGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQF
                     KLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGCL
                     QTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQD
                     SFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLNV
                     TKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQG
                     GVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERLE
                     NYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGITK
                     IEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLIN
                     SYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDIK
                     VNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEIL
                     LPSSLKSYFRL"
     gene            2786..3025
                     /locus_tag="IWJ77_002498"
     CDS             2786..3025
                     /locus_tag="IWJ77_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454049.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3041..4414
                     /locus_tag="IWJ77_002499"
     CDS             3041..4414
                     /locus_tag="IWJ77_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9454050.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4418..6052
                     /locus_tag="IWJ77_002500"
     CDS             4418..6052
                     /locus_tag="IWJ77_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9454051.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6215..>6413
                     /locus_tag="IWJ77_002501"
     CDS             6215..>6413
                     /locus_tag="IWJ77_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454052.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2246 a         1049 c         1291 g         1827 t
ORIGIN      
        1 ttgagaactt tttctacatt aaacaagaaa accgtttctc atattttttc tactgcagat
       61 tctcaagcaa ttattaacta tttagaaaaa cagaaattgt ctgaaggtac agtaaacata
      121 acttataatc ctattaaaca agaggtgatt acacaaaaaa agcattctgt gagagacaat
      181 gatattgaaa aaattcggtt tgtatttatt gatattgacc ctaaaaggaa agagggaagt
      241 gcaacagata gcgaaaagaa aaaggcagaa aatgttatgg aacaagtaga acgttatttg
      301 aaagaaaaga gaatagaatc atttgtaaaa gttgatagtg ggaatggcta ccatattttt
      361 cttcccataa atgaacaacc aaataatcac gagacgattc ttactattca gaattttctt
      421 caattacttc accgaagatt tggagtagaa gatgaagttg atattgatac ttctgtttat
      481 aatccttcaa gattatgcaa attagttggt acaccagcgg tcaaaggaaa gtccacaaaa
      541 gagcgtccgc atcgtttatc tagtttcctt tctattcccg ataacataga tagaagtaat
      601 tgttttaaaa ttcttgaaca gatagttgaa gaaaattcca tgatgtcaac ctctagagta
      661 aaaagccatc catcttttag agaagtgtta ccttttattc aagcagacta taaacaatgg
      721 ttagctcact atcctgaatt aacttatacc ataaaagagg gcgatcataa aggagttacc
      781 ttacttatat ttgacgaatg tccattaaga aaacacacga ataattctaa tggttcttgt
      841 ttgtctgtta cggctgataa taagattcga tttcattgtc tgcatgcttc ggataaaaat
      901 aaagatattc aagactttgt aaaaaaatat ccgttgccaa aacctgtaaa tccttctgta
      961 gaatcattag aatccaatca agaatattat tttgaccagt ttaaactgaa tcaagaagga
     1021 gtatttttgg tgaaagaagg tcaagaagaa aaagttgctt gtccgatgtt tattaagcaa
     1081 atacgcaaaa atatcgaaca aaaagtcatt acacttgttc ttgcctatta tgtcgatcat
     1141 tgttgggatg aaatagaaat agaaggtgga tgtttacaaa ctaataattt taaacaatta
     1201 gcaaaacatg gcttaatcat taaagctaga agagaagctg aagtcgttga ctttttacag
     1261 attcaacggg agaatgctcc aatctttttt gaacatgaat ctcttggctg gaaaaaagaa
     1321 aatgaagcaa tgatttttca gttacaagat tcttttggac gtgaaattcc ttctgtatta
     1381 actaaaaact catgctacca attaacaact aatggtacag aagcggaatt tgacgcattg
     1441 gttcataagt atgttttagg aacgaactta gaacttgctt ggtcgattgg tattgcttct
     1501 attgtgttag gctatttaaa cgttacgaag gctaatactt ttttgagttt actaattaac
     1561 ctacagggca agagcacaac gggtaaaacc actacgttac actttattgc ctctttattt
     1621 ggaaatccaa ctacggttct tagaaatatg aatgctacca ataaagccat tataaagcta
     1681 gcatcaaaca atcagggagg tgttccacta atactagatg agttaggagc agcaactcaa
     1741 aataacttta gctcgttgat ctatcaatta gcttcaggag aagaaaggct aagattagat
     1801 tctaattgtc agcttatgcc acaagagact ttttctgttt tgacccttac ctcttctgaa
     1861 gagcgtttgg aaaattattt caatgagaaa ttaactggtt tgaaagttcg atatatcgaa
     1921 ttttctgata tcaagtttac aaaggatgcg aatcacgcag agaaagttaa aagtctttct
     1981 tctaatactt ttggttgggg tataaagtta tttcttttga aactctttga acaagggatt
     2041 acaaaaattg aagcaacttt tgtcgaagct caaaaaaaac tatgggctgt tttacctgaa
     2101 cattctttga agcaacgcat tggtaataat ctagctttga tttatagtgg agccctccta
     2161 attcaagata tactgggcta tcctttacga ttagagcata tacagacggt gcttattaat
     2221 tcgtacgaac agttaattgt agatactagt ttaggaagtg agcaaacggt atttgaccga
     2281 gtgaaaacat tgttggtgat gaatagccat aaatttttaa caaacagtga caacaaacat
     2341 acgcccagta cgatttgggg aaaagtatca attcaaaaaa atggggatat taaggtaaat
     2401 atttttccta tcccgtttga ggacttgtta aggcgagaat ttcaagtaaa ggatctaacc
     2461 tatcttttta ggcaattgct tgattcgggt aatatgtgtg cagaaaaagg gagaagaacg
     2521 aaacgtattc acatgaatcg tcaagttctt ccaacatacg agattctatt accttcttca
     2581 ctcaaatcgt attttcgatt gtaatttaga ataaaccaaa gtatgaaagt gaggcaccat
     2641 gttatggaaa tatttcttat tgatttagca aaacaactga ttacttatct gtatgatagc
     2701 gagcaaaccc aaacggaagt aaatgaagct tgatctatgc ttcatttact tcattcaatc
     2761 aaacatgaaa gaaaggaaaa ttctaatgac taaaaataaa atgacactaa aagcagaggt
     2821 tcttctgtat attcaagaac atttttcaaa tcaagccttt tttacaaaac caatttactt
     2881 ggatttcgaa ataagagggg tatctgctgg atctatcggg ggtactcttc aagcgttgaa
     2941 gaacgaagga tatttagaaa atcactttgt gcaacgttcg ttcaatagaa gagtcgtgaa
     3001 aaaatggtat ttagttcatt catagagagg ggaatcaagc atgcgtgtgg cttgttatga
     3061 tcgaacgtct gtttatgaag aagcgaaaat tagtaatgac gctcaatatg atgcagtgaa
     3121 agagtatgtt ttagagaaat atgaacaaat tccccaacga ttttcagaca aaggagaatc
     3181 aggggaaaaa gcggagcgtc cagcttttcg acagcttaga gcttgggttt gtgcgtcaga
     3241 agaagagggg gtgatctatg tttggcgtta tgatcgattg gcacgtcaag ccaaaatagc
     3301 cttagaattt attgagttgt gccaacaaca tcaggttcaa gtgtattcga tttctgaacc
     3361 tcttcctgct tctttctcac acgcgtcaac gaataaacta tttgtgcaat tattgttcac
     3421 ttttgcagaa caacaacgaa acgtcattat tgagaatgtt cgtgccggac tttcctataa
     3481 aaagcaacaa cggcaatatt tgtcttctgc ggtaccatat gggtatcgtt tagtgaatgg
     3541 gaaaatccaa caagaagtac acgaagcaag gattgttcgc tatatttttc aactttattt
     3601 gaccaaaaaa tatggctaca aaaagctttg tcaacgattg acgcaacaaa agcttttctt
     3661 ccgcgaacgt ccttttcagc cctatcatat ttatagtatc ctaaaaaatc cattgtacta
     3721 tggcgaaatc aaaggaggat ctttggggaa gtatctagga acctttgaac cgattctctc
     3781 gaaagcaacc tttttacaag cccaagagat tcgccaatca aggtgtacag ctaaaaaaga
     3841 tacgtatctg tacttattac gtcaaaaaat tcggtgtccc ttctgtggtc gtcatctgag
     3901 ttcgaagtat caatggaata caaagaagac gaagacgctt cattattatc attgtaccga
     3961 ccgaagctgc caaggaattt ttcttcgggc agaaagggtt gagtcagaag tattggaaaa
     4021 gatacaggct ttttttcaat acgaaggcgt ctatcaagcc ttgattgccc aattaacgca
     4081 acaattagcc gaaaagcgaa aagatgaaaa acgcaaagta atccaacaaa agaaacaaga
     4141 agcacaatgg attaaggcat ttgaagaggg caattgttca ctggaagaac tcaagcaaca
     4201 actgcaagct cttcaaaagc cgatgtctac ttcttcgttt ctttcactcc aacagtgtga
     4261 agcaaaagtg aaagaatggc ttcaactaaa agacctctct agtcgacaat tattatgtga
     4321 ctatgtttgt cggattgagg taacaaagga taaaacagtt tgtgggattt acttccgaca
     4381 actgacacaa aatattttag aaaggaacga atgatctatg gaaatgatac aaaccaaacg
     4441 agtcgcattg tatattcgcg tgagtacaca agaacaagct ttagaaggac atagtattca
     4501 agcacaagaa caaaatggac gacagtttgc cgaacggatg ggctatgaag tggtagaagt
     4561 ttatgccgat gaaggaatca gtgggaaatc cacaaaaaat cggttcgcct atcaacgaat
     4621 gatgaacgat gctcggaacc atcgttttga tctactagtg atttggaaat tgacccgtct
     4681 cggacgcaat atgttagatg tcttacaaac agtcgaggaa ttattgcgat atgacgtagg
     4741 attacattcg atctcggaac aatttgacgt gaccacttcg acaggtaaat tgatgttaca
     4801 actccttggg agtttcggtg aatttgaacg caaccaaatt gcggagaatg ttcaaatgtc
     4861 aatgcaaagc ttagtgaaag ataaaaagcg ttatgcagga ggccgacgtt taggctatgt
     4921 cagtggtatt gacacgaatg gtcaaaaaca attggtcatt gaaccagaag aggcgaaaat
     4981 tgttcgtctg atctatgcaa aatatttagg cggagcaggg tatcgagcga ttgccaattt
     5041 tttgaatcgt caaggatatc aaacagtcaa aaagaatgct ttttctacca cggcagtcaa
     5101 agatatctta cgcaataaaa tttatgcagg gtatttagaa tatgcgcgct acatcaattg
     5161 ggaaaccaaa cgacggaagg ggaagaatcc gagaccgatt ttagtgaaag gaacccatga
     5221 accgattatt tcggaagaag attatcaaaa agtacaagaa cgccttgctt tagaaggaaa
     5281 acaacccaaa tggaaccata caggtgaaaa tgtattgaca ggcttgttac gttgtccaga
     5341 atgtggcgca ccgatggcag cgagtaatgt cacaaatacc ctaaaggatg gcacaaagaa
     5401 acggattcga tattattctt gttcagtctt tcggaataaa ggagcaagtg tgtgtcatgc
     5461 caattcgatt cgtgccgaac aagcggaaac atttgtcgcc gaccgattaa aggagatcgt
     5521 tcagctacca gaagttcttc cccgattggt ggcagcgtta aatgaagaaa tagtgcgaca
     5581 aagccaacca ttggaacaag aactcgtggt gttactggaa cgaaaggaag aattgaagac
     5641 taaaattgaa aaatgggaag ctgctttgga agatagtcct gaattgtttc cgatgttaaa
     5701 agaccgctta gacgaattga ctgaaaaacg acgacagttg cacattcgag aaaatgaaat
     5761 cttagggatc tttcaacaag gagagccgat tcaagtcaaa gatgtccaac ggatattgac
     5821 taatttggat cgttttcttg cccagagtga gaagaagcaa atcaaagcct tgtatcgtac
     5881 ctttatagag aaaataacct ttgatcctaa ccataaggag atgttggaaa ttacgatgaa
     5941 attcacgcat gccgtggtac aacaattgaa tgagcaatat caaatagccg tatccaaaac
     6001 aaaggatacg gctatttttg tgttaaaaac accctttacg ttaacgatct agtctaggtt
     6061 atatcaaaca acgttagagg ggcgctaacg cttctctagc gatttgaaat cgttagagaa
     6121 gtagttaggg aggtccttat cataaatagg gatatttatt aatgaatatt tgaaatataa
     6181 ttgtatataa gaattaaaaa gagtggggat agatatgaaa aagatgggta agtgtatttt
     6241 atgtgaaaaa tacactgaac ttactaaaga acatgtacca cctaaaaaat cagggaatac
     6301 tggaggtaaa aagaccagaa ctggtaattt agatgatttc cttaagagcg attttacaaa
     6361 aggtgatttt ccgaagggga ttaaaaggaa acctcaagga aatgtttatt ata
//
LOCUS       DACDRK010000102         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDRK010000102 DACDRK010000000
VERSION     DACDRK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.103"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IWJ77_002502"
     CDS             334..714
                     /locus_tag="IWJ77_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9454053.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IWJ77_002503"
     CDS             696..1610
                     /locus_tag="IWJ77_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9454054.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IWJ77_002504"
     CDS             1855..2544
                     /locus_tag="IWJ77_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454055.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IWJ77_002505"
     CDS             complement(2665..2751)
                     /locus_tag="IWJ77_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9454056.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IWJ77_002506"
     CDS             3427..4377
                     /locus_tag="IWJ77_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9454057.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IWJ77_002507"
     CDS             4398..4931
                     /locus_tag="IWJ77_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9454058.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IWJ77_002508"
     CDS             5567..6100
                     /locus_tag="IWJ77_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454059.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACDRK010000103         6287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDRK010000103 DACDRK010000000
VERSION     DACDRK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.104"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWJ77_002509"
     CDS             <1..177
                     /locus_tag="IWJ77_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454060.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IWJ77_002510"
     CDS             190..690
                     /locus_tag="IWJ77_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9454061.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IWJ77_002511"
     CDS             787..2634
                     /locus_tag="IWJ77_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP9454062.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IWJ77_002512"
     CDS             2637..3533
                     /locus_tag="IWJ77_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9454063.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IWJ77_002513"
     CDS             3583..3972
                     /locus_tag="IWJ77_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9454064.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="IWJ77_002514"
     CDS             3959..>6287
                     /locus_tag="IWJ77_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9454065.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DACDRK010000104         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDRK010000104 DACDRK010000000
VERSION     DACDRK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.105"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWJ77_002515"
     CDS             725..811
                     /locus_tag="IWJ77_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9454066.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWJ77_002516"
     CDS             complement(932..1621)
                     /locus_tag="IWJ77_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454067.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IWJ77_002517"
     CDS             complement(1628..1906)
                     /locus_tag="IWJ77_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454068.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IWJ77_002518"
     CDS             complement(1866..2780)
                     /locus_tag="IWJ77_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9454069.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWJ77_002519"
     CDS             complement(2762..3142)
                     /locus_tag="IWJ77_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9454070.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWJ77_002520"
     CDS             complement(3592..3879)
                     /locus_tag="IWJ77_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9454071.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWJ77_002521"
     CDS             complement(3899..4123)
                     /locus_tag="IWJ77_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9454072.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWJ77_002522"
     CDS             complement(4763..5296)
                     /locus_tag="IWJ77_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9454073.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWJ77_002523"
     CDS             complement(5317..6159)
                     /locus_tag="IWJ77_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9454074.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDRK010000105         6150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDRK010000105 DACDRK010000000
VERSION     DACDRK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.106"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..441)
                     /locus_tag="IWJ77_002524"
     CDS             complement(<1..441)
                     /locus_tag="IWJ77_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9454075.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSS"
     gene            complement(443..1111)
                     /locus_tag="IWJ77_002525"
     CDS             complement(443..1111)
                     /locus_tag="IWJ77_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9454076.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1241..1825)
                     /locus_tag="IWJ77_002526"
     CDS             complement(1241..1825)
                     /locus_tag="IWJ77_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9454077.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2156..2389)
                     /locus_tag="IWJ77_002527"
     CDS             complement(2156..2389)
                     /locus_tag="IWJ77_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9454078.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2404..3354)
                     /locus_tag="IWJ77_002528"
     CDS             complement(2404..3354)
                     /locus_tag="IWJ77_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9454079.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3351..4193)
                     /locus_tag="IWJ77_002529"
     CDS             complement(3351..4193)
                     /locus_tag="IWJ77_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9454080.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4195..4914)
                     /locus_tag="IWJ77_002530"
     CDS             complement(4195..4914)
                     /locus_tag="IWJ77_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9454081.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            5606..5764
                     /locus_tag="IWJ77_002531"
     CDS             5606..5764
                     /locus_tag="IWJ77_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9454082.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            5836..>6150
                     /locus_tag="IWJ77_002532"
     CDS             5836..>6150
                     /locus_tag="IWJ77_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9454083.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTK"
BASE COUNT         2150 a         1064 c          806 g         2130 t
ORIGIN      
        1 acttgatagc gaaaaaatgt ttttatctaa tatatttgaa agatctaata attcagctaa
       61 ttcattgatt cttttatcca tatgctcttt tgtaaatcca aagttttcca tcgaaaatac
      121 taactcactc gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac ttacgtagcc
      181 aatatttttt gatatacttt cttgtgttaa ggattgtagc gagttatttt caaataaaat
      241 ttccccaacc aattttccgt cataaagtct tggaataagt ccattcatta actttaatag
      301 tgtggatttc ccactcccag aagaaccagt aatcaatgta agactgcctt tttttattga
      361 aaaattaagg tcaacaagcg cttgttcctt aacatcctta tatgaaaaag aaacattgtt
      421 aaattcaaac aattctttca cctcaaatca aaatacttaa gaaaaaaata gtaacggaaa
      481 acaggatgat atagtctaat aagctcatgt ttgttttata gtaagaactt cttttcacag
      541 gagattctat cccctttgtt attgcattag cggtcatttc ttcagcaatc atagttgcac
      601 gtataagaac gggaactaac agaaattcca aatattgcag aggctgaaaa aaagacaagc
      661 gaatgtttct catttttaaa gattctttaa tcattcttat ttcagaacga taagtcggaa
      721 agaatctgat cactactgta atacttaaca ttattttttt cggtaaatgt attttttcta
      781 aagaggataa taattcattg ctagtataga cactaaccaa caaccatgag atcattacga
      841 ccggcaacat tcgaaataca atatataagg atgtcgcaaa aaatatagta aaaaactcta
      901 tcttgatagc atcaataatt ttcattacta aaaacagaac tgaataaatc aagatgaaat
      961 aaatacatgt ctttatttta cgaaacatca atactaaaac tgtagcaata atgaaacacc
     1021 ccacttgaaa tatatttccc tttcctaaaa aaataatgac cggaaaaaaa atatttaaag
     1081 taatcaaggt taacggattg atttttgaca ctctttcacc tccaaaaaat aagatcgcac
     1141 tgttatctta agctgataaa aatgcaccgt tattcagata acagtgcgat cttatcaaaa
     1201 agcataactg agaacaattc tcaatgcaag ctttttttaa ttaactcagt aattcaatcc
     1261 acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt tcttttgaat
     1321 agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag aagtggattt
     1381 cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac ttatctagac
     1441 aacagactgt ctttttcgtc acaaatgatg taattttttc aacaatatct tgatatttgg
     1501 ttccagctga ttggaataaa atcaaatcga atatacgttt attctcaaaa atataattaa
     1561 taaatggatc taattcattg aagttctctc tccaactttt ctctatatct atattttcta
     1621 agtttaagag gtatttggta aactcttcat tatacatatt aataagcgca ttaatatgcg
     1681 gctgaataat ttcttcaaat aacgcttcct tagaagtaaa atgtttataa aaagcacccg
     1741 tagttacgcc tgcggtcagg cacagttgcc ttatcttagt ttgctgaaat ccttcttttt
     1801 caaaatggct tagtccactt tctattaact tttggtgagt tataatataa tctctggttt
     1861 ctatgggtac ttcctcctaa taagatgaat taactttatt atcttacaca ggacttgaag
     1921 tttcaatcgt taacgtttat tactgaactc attccttaga gctaaactaa tgagaaagag
     1981 cagaataact gatacaaaaa gcaatcctac aagaattaat agactcatct aaatcattca
     2041 ccttttctaa aataatccga gaaagattta aaaaaattat atccagctat taggataagt
     2101 cctatcataa tcactccaat agccattttc ttatcctccc taaaaataaa gtcttttact
     2161 taaaaccttt tttgtaatcg tcaataaatt ttttcccttt atcagttaat tgaaatccta
     2221 tatctaaata gtcctcatat ctaagtttag ccagcatctc acttacagat gctggactaa
     2281 cacttagaac ttttgctaaa tcattatttt ttaatttctt ttttcttttt gataattcct
     2341 gaattgctag taaataaatt agtatagtag tcgttttaat ttgtaccata cctaccctcc
     2401 aaattatttc gaaaggcctt cagcaatttt atccaggttc catttcatca tcgcatagta
     2461 actatcacca tcttgtcctt tttctgcaat tgaatcagta aaaatcgttg aatagatagg
     2521 aatattggta tcttttgata ctgttttcat tggtctatca tccacactgc tttctacgaa
     2581 taaggaggga acttttgttg tgcgtaattt ttcaactaag tgttttattt gatctggtgt
     2641 tccttcttct tcagtgttga tttcccaaat gtaagcagaa ggcacattat acgctttcga
     2701 gaaatattta aagcatcctt cacttgtaac aatcattttt ttatcattcg gaattgaagc
     2761 aaatttagat ttagcttctt tgtctagaga atccaatttt tcaatatact tatctagatt
     2821 ttctttatag aattttttat tatcaggatc tttttccgct aactgttttt caatattttt
     2881 agcgtaaata ataccgtttt ctaaatttaa ccaagcatga ggatcttcct tccctttctc
     2941 actctgaccc tctaagtaaa taacatctat gccatcactt gctgcaaaat agtctttgtt
     3001 ttcctctttg ttcgcatttt taactaattt tgtaaaccaa gcatttcctc cagtttccaa
     3061 gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt ttttgaacat cttcaggcaa
     3121 aggttcatat tcgtggggat ctttcccaat aggtacgata ctgtgtaaat caattttatc
     3181 ttttgctata ttttcggtaa tatctgctaa aatcgaatta gtaactacta cttttaattt
     3241 ttcgttcgaa gccactttat ctttgtcttt actatttatt gaacatgccc cgaatataaa
     3301 aagagcaatc aaaatcccca gacttcccag tactgctttc cattttctca tcataacgat
     3361 ctctccttct ttcttaaaaa tttttgtttt ggtgagatta gaaaactaat tacaaataag
     3421 aatgccgctg ttaaaacaat acttgatcct gctgctatgt taaaactata accaataaac
     3481 aatccaagca atgatgaact tgctccgact gttgcagaaa taaatatcat agttttcaag
     3541 ctatttgcat ataaatatgc tgtagctgct ggtgtaatta acattgcaac aattaatata
     3601 gttcccacac tttgcatagc tgttactgct actaaagtta atagaaccat caatagataa
     3661 tgataaaaac tgacattcat ccctattgct tttgccatta atggatcaaa agaagtgatt
     3721 aataattctt taaaaaatag aactactata accaacacta aaaccgaaac gccaattgta
     3781 atccacttat caatatcttg tacagctaaa atatttccaa ataaaatatg aaataaatcc
     3841 gttgagctat ttgcaactcc tataaggata actcctagtg ctaaaaaaga actgaaagta
     3901 ataccaattg ctgtatctcc ttttattaca ctattgcttt ttataaaagt aataatgata
     3961 gaagctaata gaccaaatac aatcgcacca ataaaaaaat taatacctaa aatataagaa
     4021 attgctactc ctggcaaaac agcatgagaa atagcatcac ccataagaga cattcctctt
     4081 aaaataataa aacaacctac tgttccagaa acaatcccta taactacaga tgtaattaga
     4141 gcattctgta aaaagtgaaa gtcatacaga ccattaataa aattttgaag catattattc
     4201 cccctctcca ataaatatag tatctccata ggcttttttt aaattttctt cattaaatac
     4261 agattctaca cttccgtgag caattagatt tcgattcaaa agaataatat tatcaaaata
     4321 cttctttacc ttgcttaaat catgatgcac aattagcact gtttttccct gatgtttcaa
     4381 tttttttaac gtatccataa ttattcgttc actaactaag tctatcccta caaatggttc
     4441 atctaaaaat atgaaatccg cattttgagc aagacatctt gctaaaagta cacgttgaaa
     4501 ttgtcctcca gataattctc ctatttgatg attagaatat tttcccatat cgaccttttc
     4561 caaagccttt gaaactcttt gccattctgc atttttaatt ctttgaaaaa ctttcattcc
     4621 ggcatatgtt cccatagata cacattcttt tacttttatt ggaaaagtaa aatcaatatc
     4681 agctttttgc tctacatatg caatcttcac cagtttttta ttgatatctt cattattaat
     4741 ggagacctct ccctgatgag ggataatatt taacattgct ttaagcaatg tggattttcc
     4801 agcaccattt ggaccaataa tacctgttaa agcaccttct tgtattttta aagaaatctc
     4861 gttcaatgct aaaaaatcgt tataacaaac attaagcttt ttcacttcta acataacttt
     4921 ctccttctaa atgataatga ttatcacgat catatggtat acaaaaatta tattttagtc
     4981 aaccctaaat tttatagttt agattttttt ctgttgctga agattagaat gacaaaacta
     5041 tttgaaacta cgcgtatagt aagtagcttt caaaatatat aaaaaaggag cgttctcaaa
     5101 tgagaacatg ctcctaaaac tactttattg gcgtatggaa aaattatact tgaagtggat
     5161 agcgggttga ataagttgat aagcatcaaa acatttataa aaattattca agactatgtc
     5221 tgttccatat acaaatatga aaatcagtaa actccattta acttttttcg tagtgtgtct
     5281 ttttatacat gtttatcgtt attaatagtg aataggaaaa aaactatttt tgatcattga
     5341 caattaaata caatttttga ataacgatcc ctgctattcg agctagaaac tcaagtacgc
     5401 catgacctga ttagcgacaa atacttagag atttcttttt gttctgggaa aacaaaatgc
     5461 catgacaata gcagcaaata atgatacttc cgtaaggctc gccgtaaact ggggagaggt
     5521 gaaattccta tgtgcttacc caaagcaatt caaaaatttc ataactacta ttttaggaga
     5581 tgattttata gaatccagag tctacctgaa taaaaaacaa acctgtgcat ttctaggaat
     5641 cgataataat actctagata agtggattcg tatatataat ttcccagtaa taagagttgg
     5701 tggtgtagtt cgctttgacg tagaagatat taaagacttt atgaatacat ttaaaataac
     5761 ttgaaatttt cttctcgccg catatactaa aattagtgaa atatactgcc ttactttttt
     5821 taaggagtga ggattttgcc tacaaagctt tcaaatggga aatataaaac taatctgaga
     5881 tatccaaaaa ggtttaaaga aataacagga attgcatcag aaaagtatca aaaaacattt
     5941 ccaaataggc aattggcgat taaagccgaa aatgatatga agaagaaaat agaaaaagtt
     6001 ttgagagagg agaatgcaaa ctcattagaa ttaaaaggaa aaataacgtt taaaaaattt
     6061 tacgaatcta aatggcttcc tagatatgaa ttaggtcaaa caattagatc aaacaggcct
     6121 cctagtgata taaccattag taatacaaaa
//
LOCUS       DACDRK010000106         5966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDRK010000106 DACDRK010000000
VERSION     DACDRK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.108"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(170..3319)
                     /locus_tag="IWJ77_002533"
     CDS             complement(170..3319)
                     /locus_tag="IWJ77_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9454084.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="IWJ77_002534"
     CDS             complement(3333..4550)
                     /locus_tag="IWJ77_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9454085.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..>5966)
                     /locus_tag="IWJ77_002535"
     CDS             complement(4540..>5966)
                     /locus_tag="IWJ77_002535"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9454086.1"
                     /translation="DTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVL
                     ADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIK
                     KLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKER
                     RFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEM
                     NLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKAD
                     FAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFG
                     TSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKY
                     AHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAI
                     SSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1629 a         1266 c          998 g         2073 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcat
//
LOCUS       DACDRK010000107         5903 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDRK010000107 DACDRK010000000
VERSION     DACDRK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.109"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..985)
                     /locus_tag="IWJ77_002536"
     CDS             complement(<1..985)
                     /locus_tag="IWJ77_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP9454087.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="IWJ77_002537"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="IWJ77_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9454088.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="IWJ77_002538"
     CDS             1810..2964
                     /locus_tag="IWJ77_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9454089.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="IWJ77_002539"
     CDS             3179..4147
                     /locus_tag="IWJ77_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9454090.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="IWJ77_002540"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="IWJ77_002540"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9454091.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..5785
                     /locus_tag="IWJ77_002541"
     CDS             5177..5785
                     /locus_tag="IWJ77_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9454092.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1782 a         1149 c         1335 g         1637 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc ggcagtgcca ttgatcttac gctttatcga ttagacacgg gtgagcttgt
     5581 accaatgggg agccgatttg attttatgga tgaacgctct catcatgcgg caaatggaat
     5641 atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc atcatggaaa acagtgggtt
     5701 tgaagcatat agcctcgaat ggtggcacta tgtattaaga gacgaaccat accccaatag
     5761 ctattttgat ttccccgtta aataaacttt taaccgttgc acggacaaac tatataagct
     5821 aactctttcg gcaggaaacc cgacgtatgt aactggttct tagggaattt atatatagta
     5881 gatagtattg aagatgtaag gca
//
LOCUS       DACDRK010000108         5812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDRK010000108 DACDRK010000000
VERSION     DACDRK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.110"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            37..1044
                     /locus_tag="IWJ77_002542"
     CDS             37..1044
                     /locus_tag="IWJ77_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9454093.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1056..2060
                     /gene="neuB"
                     /locus_tag="IWJ77_002543"
     CDS             1056..2060
                     /gene="neuB"
                     /locus_tag="IWJ77_002543"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9454094.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2057..3205
                     /gene="neuC"
                     /locus_tag="IWJ77_002544"
     CDS             2057..3205
                     /gene="neuC"
                     /locus_tag="IWJ77_002544"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9454095.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3178..3855
                     /locus_tag="IWJ77_002545"
     CDS             3178..3855
                     /locus_tag="IWJ77_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9454096.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3845..4987
                     /locus_tag="IWJ77_002546"
     CDS             3845..4987
                     /locus_tag="IWJ77_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9454097.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5000..>5812
                     /locus_tag="IWJ77_002547"
     CDS             5000..>5812
                     /locus_tag="IWJ77_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9454098.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYD"
BASE COUNT         2156 a          745 c         1128 g         1783 t
ORIGIN      
        1 attatagatg actataattc ataggaggag atataagtgt ttaaaggaaa aacaatatta
       61 gtaactggcg gaactggttc cattggaagt gaaattgttc gtcagctttt aaaatatgaa
      121 ccaaaagcta tcagaattta tagtagaggt gaagaaaaac aattttatat gcaacaagag
      181 ctctcaaatc acactaattt gagattttta attggtgata tcagagactc tcaaaggatt
      241 gattatgcat gtcgtgatgt ggatattatt tttcatgctg ccgcaatgaa acacgttcca
      301 gcaagtgaat ataatcctat ggaagctgta aaaacaaatg tgatgggaac tcaaaatgta
      361 atagatgcag cgatcaaaaa taatgtaaaa cattttgtag gtatcagtac agataaagta
      421 gtgagtccta ctaacaccat gggtgcgaca aagttattga gtgaaaaatt aataatagct
      481 gccaatcagt ataaaggaaa aacagaaaca atattttctt gtgtaaggtt tggaaatgtc
      541 atggggtcaa gtggatctgt tattcctctt tttagtaaac aaataaaaaa caataaagat
      601 attactttga cagatgttga tatgacacga tttatgatgt caataccgga tgctgcaaga
      661 ttagttctgt ctgctgctat gaattcaaaa tttggagaaa catatgtctt aaaaatgccg
      721 gcaatcaata ttttaacttt agctcaatca ttaataaaaa tatataatga tacacatgat
      781 gaaaaatata gaggtgaaat aaaagttgta ggtatcaggc caggagaaaa attatatgaa
      841 gagctaatga ctacagacga aattgaacgc tcatatgaaa atgataaaat gtatatcatt
      901 ccaccacact atcatgctac tgacgaactg tacaaagatt atgaaaaaat taaaatcact
      961 gcatactctt cagatgcaac agaattaatg agtggacagg aatttattag aatcgcaaaa
     1021 gagaataatg tcgtgacttg gtaaggagtt ttatgatgaa atcagaagta tatgtaattg
     1081 cagaaattgg tgtaaaccat aatggaagtc tagatttagc tttagaaagt attgataaag
     1141 ctaaagaatg tggagctaac gcggttaaat ttcaaacatt taaaacgaat aagctagttt
     1201 ccaaaaaggc tccaatggca aaatatcaga aggataatct taaagaggac agttctcaat
     1261 ttgaaatgct aaaaaaatta gaattatctc aatctgattt tattagaata aaagattatt
     1321 gtatacaaaa taaaattgat tttttgtcta ctccttttga tgaagaaagt gcagactttc
     1381 taaaacaaat tggtgtacaa ggatttaaaa ttggctcagg agatttaaca aatttacctt
     1441 ttcttagaaa aatagatatg tatgggttgc cagttttatt atctacaggt atggctgata
     1501 ttcatgaggt cagtgaggct gtagattgtt tcatagatag tccagttact attttacatt
     1561 gtacttcaaa ttatccggct agttctgaag atattaattt attggcgatt aacacattaa
     1621 aagaaatgtt cgggataccc gttgggtact ccgatcattc attgggatat gatgttggta
     1681 tttgtgctgt tgcgatgggt gctaaggtaa ttgagaaaca ttttaccttg gataaggaac
     1741 tacctggtcc agatcataaa gcttctttag atcctcagga atttgcagat tttgtaaagc
     1801 atattagaaa cactgaaata tttttaggtg atggtgttaa gcgagcaatg ccttctgaac
     1861 ttagtacaaa agaagtatca agaaaaagcc tagtggtcac tgagaattta aatgtgggag
     1921 atattcttac agagaaatct cttacaataa aacgtcctgg aaatggaata gctcctaagt
     1981 ttttatatca atatattggc aaaaaagtta aaagaccaat aagtgcagat agtgttctgc
     2041 ttgaggaaga tgttgaatga aaaaaatact tatagtaagt ggaacacgag ctgattatgg
     2101 aatatataaa cctgttgctg agaatttact acaagataat tgggatgtat catttgttgt
     2161 ttctggaatg catttatccc ataaatatgg atttactaaa gaattgatct tagctgacgg
     2221 atttagaatt agtggagaag ttcaaagttt gtttttagag aacaataagg gaaacatggc
     2281 tcggtctgtg gcattagaga taaatggttt cacaaatata tttgaacaaa ctagccctga
     2341 ttttgtatta ttactagggg atagaggaga aatgttggca gctgcgatag cttgtttgta
     2401 tcttggaatc aaaacagcac atattcacgg aggcgaagtg tctggtactg tagatgaatc
     2461 aataagacat gccataacga aaatagcatc tgttcatttt gctgctacgg aaaaaagcaa
     2521 agagcgttta ataaaaatgg gggaagattc ttggagagtt tttcccgtcg gagctcctag
     2581 aatagatact attttaaata atagattacc tagttttaga gatataaaag aaaaatatca
     2641 tctaaaattt gaaaagaaaa aatacgtttt gcaagtattt catccagttg taaccgaact
     2701 agaaaatata gaaaaacaaa ttttagcttt gatcaattca atggaacaac aatctcaacc
     2761 aattatttgc attttaccta atagtgatgc tggtagcgaa attatcagaa aaatgtacca
     2821 gaaatattta aaaacggata ttatttatat tacgaattta gagccggagg aatacctgac
     2881 tatattaaaa gagtgcaaat ttatgatcgg aaattcttct tctgggataa ttgaagctgc
     2941 atcattcaaa ataccagtaa taaatttagg aaccagacaa aatggacgag aacaatctaa
     3001 aaatattata aatgcaaatc taaatgaaaa tgaaattgaa actgctttga ataaaataca
     3061 agatcctcag tttataaaaa gcttgaaaaa tgttaagaac atctacggtg acggcaacag
     3121 ttctagaaga ataacaaaaa cattgaatca aatattgaat gatggggaag atattaagtg
     3181 gattcaaaaa caaatagctt attaaaatat actagtgatc catacattat tattggggct
     3241 ggcggacacg caaaagtcat tattgaaacc ttaagattag aaaataagtt aatatatgga
     3301 ttttttgatg ataagcaaaa taaaatgcaa gatctgaaat atcttgggaa aataaacgaa
     3361 ttgaatagct gggcggatag caaaaaaaaa atctataaat ttattgtagc tattggtgac
     3421 tgtgtagtaa gaaaaaagat tataaaaaaa ttgaatatgc caagtgagca atatggaata
     3481 gctgttcatc caagtgctat agtggcaaaa gaaatttcaa ttggttatgg aactgttatt
     3541 tttgcgaatg ctgtaattaa cccagatgct gtaattggag agcatgctat aataaacaca
     3601 ggtagcattg ttgagcatga ttgtagaatt aataactatg ttcatttatc tccaggtgta
     3661 tgcttagcgg gtggagtaca tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt
     3721 atccagatga agaaaattgg atcttggtgt atgataggcg cagggagcac tattgttaag
     3781 gatattccct cacattcctt agcttatggc aatccagcta aaattaagaa agaaggaatt
     3841 aattttgaat tctagaattt tattatcaag tcctcatatg ggaggaacag aagaaaaata
     3901 tgttgctaag gcttttaaag ataattggat agcgccatta ggtgaaaatg tggataaatt
     3961 tgaagaaagt attacaaaat atactggttc taagggcgga gcttctgcgt taagttcagg
     4021 tacagctgcc atacatcttg ccttaagact gcttaatgtt ggaaaaaacg atattgtctt
     4081 tgctccttca tttactttta tagctacagt aaatcctatt ttatatcaag gggctgtacc
     4141 agtgttcatt gactcagaac ctaatacatg gaatatgagc cctgttgcac tagagagcgc
     4201 attagaagaa gctgaaagaa acaaccaatt acctaaggca atcatcgtag tgaatttata
     4261 tggtcaaatg gctgattatg aacgaatttt agctattgca aaccaattta atgttccagt
     4321 gatagaagat gcagcggaat cgttaggttc tacttataag ggaatttcgt caggcttatt
     4381 tgggacaatt gggatctatt catttaacgg taataagatc atcacaacct caggtggagg
     4441 agctctagtg tctagtgata aaaaatatgt tgagcattca agatttttag caacacaagc
     4501 aagagatcaa gcaccgtatt atcaacatag cgaaataggt tataattacc gtctaagcaa
     4561 tatttctgcc ggtatcggac gtggacaaat ggaagtattg aatgatagag tagctaaacg
     4621 aagagaaatt ttcaatcgat ataaggaagc tttttctgga attgatagaa ttgaaatgat
     4681 gccagaagtg gatggcatgg tgccaaatcg ttggttgtct actttaacga tcaatgatta
     4741 taattctatt gatcaagtag ttaacgtaat aaataaacta aatgaaaaaa acattgaaac
     4801 aagagctttg tggaaaccac tacatttgca accagtattt caaggggtaa aaatgtatta
     4861 ccatgataat aatcttagtg tgtcagaaaa gttatttaat agcggcttat gcttaccttc
     4921 tggaagtaat atgacagata gtgaacaaga atttgttatt gatgagttta aaaaaatatt
     4981 caagtaggtg tgatatttta tgaacatagg aattattggt ttaggttcca tggggaaaag
     5041 aagacttaga ctcattcaag aacactttaa tgatatttta ctatgtggtg ttgatactaa
     5101 tgaggcgaga cgaaaggaag ttgaaaagga atttggaata ccaacttata atagtctaac
     5161 agaggcaaaa aatttgtttg aaataaattc agtttttgtt tgtacatccc ctataaccca
     5221 tgaatttatt ataaaagagg ctttaaattt agattgtaat atctttacgg aaatcaattt
     5281 attaaatgaa tattatgatg aaataatcga agtagctaaa aagaaagata gattacttta
     5341 tctatcttct acatttttga agagaaaaga aattcaatat attaagtcta aagtgacgca
     5401 taataaaaaa atttcttatc aataccatgt aggacagtat ttacccgact ggcatccttg
     5461 ggaagattat cgcaattttt ttgtttcaaa caaaaaaact aatgggtgta gagaaatatt
     5521 cgctattgaa ttaccttgga ttatagatac atttgggaaa gttaaatcgt ttactgtaat
     5581 gacccagaaa ttatccaatc ttgatattga ttattcggac agtttcactc ttattattga
     5641 gcatgaaact ggtatagttg gaactttaag tgtagatgtt gtttcaagag tagctaaaag
     5701 aaatttaact ataattggtg aaaagatttt aatagaatgg gaaggtaacc cggattctct
     5761 attttcatgg aatgaagaaa cttctagtat gacagatatt caattgtacg at
//
LOCUS       DACDRK010000109         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDRK010000109 DACDRK010000000
VERSION     DACDRK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.111"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IWJ77_002548"
     CDS             450..1604
                     /locus_tag="IWJ77_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9454099.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IWJ77_002549"
     CDS             1598..2020
                     /locus_tag="IWJ77_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454100.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IWJ77_002550"
     CDS             3143..4075
                     /locus_tag="IWJ77_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9454101.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IWJ77_002551"
     CDS             4092..4607
                     /locus_tag="IWJ77_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9454102.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IWJ77_002552"
     CDS             4903..5184
                     /locus_tag="IWJ77_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454103.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IWJ77_002553"
     CDS             5186..5602
                     /locus_tag="IWJ77_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454104.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDRK010000110         5791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDRK010000110 DACDRK010000000
VERSION     DACDRK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.112"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWJ77_002554"
     CDS             complement(99..1229)
                     /locus_tag="IWJ77_002554"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9454105.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IWJ77_002555"
     CDS             complement(1877..3079)
                     /locus_tag="IWJ77_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9454106.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IWJ77_002556"
     CDS             complement(3139..3342)
                     /locus_tag="IWJ77_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9454107.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IWJ77_002557"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IWJ77_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IWJ77_002558"
     CDS             4879..5691
                     /locus_tag="IWJ77_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9454108.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          922 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta g
//
LOCUS       DACDRK010000111         5760 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDRK010000111 DACDRK010000000
VERSION     DACDRK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.113"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IWJ77_002559"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IWJ77_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9454109.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="IWJ77_002560"
     CDS             complement(2064..3062)
                     /locus_tag="IWJ77_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9454110.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="IWJ77_002561"
     CDS             complement(3288..3941)
                     /locus_tag="IWJ77_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9454111.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            4435..5727
                     /locus_tag="IWJ77_002562"
     CDS             4435..5727
                     /locus_tag="IWJ77_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9454112.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT         1729 a         1207 c          931 g         1893 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcgg ctctatactt
     4321 tttgatgttg gtggatgaca ccagcatctt ttttattttc caaaacatat agaaaagaca
     4381 ccttgaaatc ctttagaatt aatttggcga accaacccaa aaggaggaat caagatgtct
     4441 tacactcacc ttatcaaaga aacattagat attctagact tatcagttac ttttaacgaa
     4501 aattgtctca caaaagaaaa atacaaaggg caaatctgtc atatttatcg tggaaacttg
     4561 atttatacag cccaagaatg catccactgt aaacaccaga ttgcttcaga tattgtgcgt
     4621 tggggtacaa caaccgtccg gttgttaatg aacgatgttt ctgaataccg tacctacctc
     4681 gaattgaaaa agcaacgctt caaatgcaaa gcttgtcagc ggacctttgt cgcagatact
     4741 tctgttgcga aaaaacattg tttcatcagt gagaaagtcc gctggtctgt cgtgactcgt
     4801 ctgaaaaaaa atacctcaat gacagagatc gccgctcaga aaaatctatc ggtctcttct
     4861 gtttaccgta ttatgaagcg attttatcgt ccgttgaatc cttttcggac accgttacct
     4921 aaggtacttt gttttgatga attcaaatca gttcggggtg tttcaggtgc tatgagtttc
     4981 atcatgatgg atggacaaac acagcgctta ttggacattg tcgagaaccg acaactgcct
     5041 tttttaaaac gttattttag tcatttttca cgagaaattc gtgaagcggt cgaatggatc
     5101 gtgattgata tgtacgctcc ttatgtatcg ctcgttaaaa agctgtttcc aaaagcacag
     5161 ctgattattg accggtttca catcgtccaa catattggtc gtacctttcg caatcatcga
     5221 atcaaagaaa cgaaccaact attaaaaagc aaagaacaaa aacactatca attaggtaag
     5281 caattaaaaa gatactggaa acttcttcaa aaagatgaac gcaagcttga ttatactcga
     5341 cgcctttggc gacctggctt caaagctcat ttaacagaga ccgatattgt ggatcgctta
     5401 ctgaaaggga gccctgcttt gcgtgtaggc tatcagttat atcaagactt tctttacgct
     5461 gtaaaggaac gagactacgt atcgtttgaa gaactactta ccaataatat catgcttccc
     5521 gaaggctatc agacgacctt gagaacgttc cagaaatttc tgccacaaat caaaaatgct
     5581 ttacaacaaa gttattcaaa tggcccactt gaatgtctga acaaccatat caaagtgtta
     5641 aaacgaaatg catatggctt tcgtagcttt tataatttca aattgcgcat catgattcgt
     5701 catggcaacg cattgatttt caactaaaaa agatcaagag gaactgatcc acttgatctc
//
LOCUS       DACDRK010000112         5614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDRK010000112 DACDRK010000000
VERSION     DACDRK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.115"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..2008)
                     /locus_tag="IWJ77_002563"
     CDS             complement(59..2008)
                     /locus_tag="IWJ77_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9454113.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="IWJ77_002564"
     CDS             complement(2050..3108)
                     /locus_tag="IWJ77_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9454114.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="IWJ77_002565"
     CDS             complement(3227..3682)
                     /locus_tag="IWJ77_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454115.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="IWJ77_002566"
     CDS             complement(4266..5069)
                     /locus_tag="IWJ77_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9454116.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DACDRK010000113         5247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDRK010000113 DACDRK010000000
VERSION     DACDRK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.116"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IWJ77_002567"
     CDS             complement(207..1169)
                     /locus_tag="IWJ77_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9454117.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IWJ77_002568"
     CDS             complement(1171..2610)
                     /locus_tag="IWJ77_002568"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9454118.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IWJ77_002569"
     CDS             2795..4741
                     /locus_tag="IWJ77_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9454119.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..>5247
                     /locus_tag="IWJ77_002570"
     CDS             5005..>5247
                     /locus_tag="IWJ77_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9454120.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1697 a          888 c          867 g         1795 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaa
//
LOCUS       DACDRK010000114         5247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDRK010000114 DACDRK010000000
VERSION     DACDRK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.117"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1389
                     /gene="gadC"
                     /locus_tag="IWJ77_002571"
     CDS             <1..1389
                     /gene="gadC"
                     /locus_tag="IWJ77_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP9454121.1"
                     /translation="GGILWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAI
                     FFQWFQITVGFITMIYFILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTN
                     RTAKIAKAGFVIGIVIPSIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFV
                     SFILAYMGVEASASHINELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLS
                     LSAGVIQTFETLILHFNSHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGL
                     LPVFFRKTNKQDVPVPLIMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGY
                     LLFFLGYFVLVFKKKDLKRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAAS
                     EHRTYQIILLISFVVTVLLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEH
                     HVTKNDEHILKH"
     gene            1402..2802
                     /locus_tag="IWJ77_002572"
     CDS             1402..2802
                     /locus_tag="IWJ77_002572"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP9454122.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(2967..4407)
                     /locus_tag="IWJ77_002573"
                     /pseudo
     CDS             complement(2967..4407)
                     /locus_tag="IWJ77_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4800..5247
                     /locus_tag="IWJ77_002574"
                     /pseudo
     CDS             4800..5247
                     /locus_tag="IWJ77_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1675 a          850 c         1001 g         1721 t
ORIGIN      
        1 ggaggtattt tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa
       61 ggatggaaag atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt
      121 tttgcagcta tttttttcca atggtttcaa attacagtag gttttattac aatgatctac
      181 ttcatactag gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt
      241 atcaagttta ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg
      301 accaatcgta ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg
      361 atcattctct ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct
      421 attaatgcga aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta
      481 tcttttatat tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa
      541 aatccccaaa gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta
      601 gatgcaatcg gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt
      661 gctggtgtaa tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg
      721 ttggtaaaag tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg
      781 gtagttggtc catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc
      841 ttccggaaaa ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc
      901 gttactattt gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta
      961 gtagcaatct ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt
     1021 tactttgtat tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa
     1081 actgttggca aaacgattat cggtggaatt ggttttattt tatcagtgtt cgcattgctt
     1141 atctcttttg taccaccagc ttcaattgca gctagtgagc accgaacata ccaaatcata
     1201 ctacttatta gctttgtagt gactgttctt ttacctttta tcatttatga agtacatgat
     1261 aaaagtgctc acacaactat tgaggaacct actcacgtga aagctgaaga cgttaatccc
     1321 gctgtttatc cagctgctag aggagagcac cacgttacaa aaaatgatga acatatttta
     1381 aaacattagg aggaattcat tatgttatac ggaaaagata atcaagaaga aaaaaactat
     1441 ttggaaccaa tttttggctc tgcaagtgag gatgttgact tgcctaaata taagttaaac
     1501 aaagaatcca ttgaaccacg aattgcttat caattagtac aagacgagat gttggatgaa
     1561 ggaaatgcgc gattaaactt agctactttt tgtcaaacgt atatggaacc tgaagcagtg
     1621 aaattgatga cccaaacgtt agaaaaaaat gcaattgata aatcagaata cccacgaaca
     1681 acggaaattg aaaaccgctg tgtaaatatg attgctgatt tatggcatgc tccaaataat
     1741 gaaaaattca tgggaacttc aacgatcggc tcttcagaag cctgcatgct gggtggtatg
     1801 gccatgaaat ttgcttggcg taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa
     1861 aaacctaacc tggtgatctc ttctggttac caagtttgtt gggaaaaatt ctgtgtatat
     1921 tgggatgtgg aactgagaga agtcccaatg gatgaaaaac atatgtcaat taatctagat
     1981 actgtcatgg attatgtgga tgagtacaca attggtattg taggtattat gggtattact
     2041 tacactggtc gttatgatga tatcaagggt ctgaatgatt tagttgaagc tcacaataaa
     2101 caaactgact ataaagtata cattcatgtt gacgctgcat cgggtggctt ttatgcacca
     2161 tttactgaac ctgatctagt ttgggatttt caattgaaaa atgttatctc aattaattct
     2221 tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa
     2281 caatacttac cagaagaatt agtatttaaa gttagttact taggtggaga aatgccaact
     2341 atggctatca acttctctca tagtgcagca caactaattg ggcaatacta caactttgta
     2401 cgctatggct ttgatggtta tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat
     2461 ttagccaaag agatcgaaaa aactggtatt tttgaaatca ttaatgatgg atcagaattg
     2521 ccagttgtgt gctataagct gaaagaagat cccaatcgcg aatggacact atatgattta
     2581 tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct acccattacc taaagacttg
     2641 gatcaattaa ttattcaacg cttagttgtt cgagcagact ttggaatgaa catggctggt
     2701 gattatgtac aagatatgaa ccaagcaatt gaagagttga ataaagctca tattgtttat
     2761 cataaaaaac aggatgtgaa aacgtacggc tttactcact aaaattcttc agagaaatat
     2821 aaaagaagtt aattaactaa taaaaggagt tgtgattacc acacagctcc ttaaatctat
     2881 ttgaataaat atatgatatt catttgttac actctcaatc ttagctgttg ttctattcat
     2941 cattactctt attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta
     3001 tgaattgttt gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg
     3061 tataaagaac tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt
     3121 tctctaagtt gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct
     3181 aactgttgtc gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt
     3241 ggagatttcc gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa
     3301 ttctcattcc aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga
     3361 acttctaagt acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat
     3421 atctgaatat cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc
     3481 tgattttctt cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa
     3541 gggttatctg ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag
     3601 ttatcgcata gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg
     3661 tcatgacata aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga
     3721 gtatgtcggg caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc
     3781 tgcaaaccga tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt
     3841 gaaaaaaaat gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta
     3901 tttagctgga tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga
     3961 taatcctttt caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa
     4021 aaattaatca actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca
     4081 gctatattac tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca
     4141 gagcgctgat agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact
     4201 gtaaagctac ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg
     4261 cattccaagt cgttagtgac agacagtgag tgggcaaacg ttcaattgat ctgctatttc
     4321 gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa atgtaaactt gacgaaaaat
     4381 acttttctcc agataggttt ccaacatagt actcctccta gtcattttat acataactat
     4441 tataactcta ccaatcggaa tagtcctgta atatattgaa tcaaaaatat aaattataaa
     4501 ataattagat ggggtatttg gtttaagtat cccttatata gtaataaatt tataggtgtg
     4561 attctcgaat taatacccga aaatgtgaga gaatttttat ggatttttta ggatacaaat
     4621 taattatttt tgcttttatc ctgattacca gtccagtact gtcggtatcc cattttgcag
     4681 caaaatcatg ttgataaagt gacaagaaga ctgatgagat atattatcca aattgagaag
     4741 ataattaatc ggattcttga gtatacccta ctttttaaga aagtagggta tacaagcgta
     4801 tgaataaaga cataactttc cctgtgatgg acgttcggct tgttccaatt accaaaatac
     4861 aggcgaacga ttataatccc aatcgagtag cgtctccgga aatgaactta ttggagcttt
     4921 ctatcattga agatggcttt actcaaccat tagtttgtta ttacaaggaa aaagaagaga
     4981 agtatatctt ggttgatgtg cttcataggt atcgtgttgc taaggaaaga ctcaacttaa
     5041 aagttgtacc ggtaacagtt attgataaac ccatataaga acgtatggct tcaacaattc
     5101 gacacaatcg tgcaagaggg acccatgaaa taagaggaat ggaaattgtc atccaaagat
     5161 tagtaaaaaa tggatggtca gacaagagaa tatccaaaga attaggcatg gacattgaag
     5221 aaatttttcg ctttaagcaa gtgtctg
//
LOCUS       DACDRK010000115         4948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDRK010000115 DACDRK010000000
VERSION     DACDRK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.118"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..466)
                     /locus_tag="IWJ77_002575"
     CDS             complement(26..466)
                     /locus_tag="IWJ77_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9454123.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="IWJ77_002576"
     CDS             626..1132
                     /locus_tag="IWJ77_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9454124.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="IWJ77_002577"
     CDS             1300..3213
                     /locus_tag="IWJ77_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9454125.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="IWJ77_002578"
     CDS             3218..4219
                     /locus_tag="IWJ77_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9454126.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="IWJ77_002579"
     CDS             complement(4421..4627)
                     /locus_tag="IWJ77_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9454127.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         1829 a          757 c          998 g         1364 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
     4681 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
     4741 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
     4801 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
     4861 agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc
     4921 aactgtgaaa taaaattaca aatgataa
//
LOCUS       DACDRK010000116         4891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDRK010000116 DACDRK010000000
VERSION     DACDRK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.119"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(7..1164)
                     /locus_tag="IWJ77_002580"
     CDS             complement(7..1164)
                     /locus_tag="IWJ77_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454128.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="IWJ77_002581"
     CDS             complement(1167..1523)
                     /locus_tag="IWJ77_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454129.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="IWJ77_002582"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="IWJ77_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9454130.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="IWJ77_002583"
     CDS             complement(2991..3830)
                     /locus_tag="IWJ77_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9454131.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..>4891
                     /locus_tag="IWJ77_002584"
     CDS             4015..>4891
                     /locus_tag="IWJ77_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9454132.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKN"
BASE COUNT         1593 a          970 c          722 g         1606 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat a
//
LOCUS       DACDRK010000117         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDRK010000117 DACDRK010000000
VERSION     DACDRK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.120"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IWJ77_002585"
     CDS             564..989
                     /locus_tag="IWJ77_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454133.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IWJ77_002586"
     CDS             2141..3118
                     /locus_tag="IWJ77_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9454134.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IWJ77_002587"
     CDS             3128..3625
                     /locus_tag="IWJ77_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9454135.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IWJ77_002588"
     CDS             4001..4282
                     /locus_tag="IWJ77_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454136.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IWJ77_002589"
     CDS             4284..4700
                     /locus_tag="IWJ77_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454137.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDRK010000118         4713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDRK010000118 DACDRK010000000
VERSION     DACDRK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.121"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            108..359
                     /locus_tag="IWJ77_002590"
                     /pseudo
     CDS             108..359
                     /locus_tag="IWJ77_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="IWJ77_002591"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="IWJ77_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9454138.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1767
                     /locus_tag="IWJ77_002592"
                     /pseudo
     CDS             1656..1767
                     /locus_tag="IWJ77_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1788..1871
                     /locus_tag="IWJ77_002593"
     CDS             1788..1871
                     /locus_tag="IWJ77_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9454139.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="IWJ77_002594"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="IWJ77_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9454140.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="IWJ77_002595"
     CDS             complement(2996..3859)
                     /locus_tag="IWJ77_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9454141.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="IWJ77_002596"
     CDS             complement(3861..4133)
                     /locus_tag="IWJ77_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9454142.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4365)
                     /locus_tag="IWJ77_002597"
     CDS             complement(4150..4365)
                     /locus_tag="IWJ77_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9454143.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..>4713)
                     /locus_tag="IWJ77_002598"
     CDS             complement(4457..>4713)
                     /locus_tag="IWJ77_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP9454144.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1447 a          796 c          865 g         1605 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctt gataatattt tggaaaacca
     4621 agttatcttc tttatgttgc ttatacagtt cttccaggtt tgattttatc gttgcgctgt
     4681 ccgtatcaac taaataagga acaaaaccga tca
//
LOCUS       DACDRK010000119         4649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDRK010000119 DACDRK010000000
VERSION     DACDRK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.122"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(127..420)
                     /locus_tag="IWJ77_002599"
     CDS             complement(127..420)
                     /locus_tag="IWJ77_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454145.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(566..1117)
                     /locus_tag="IWJ77_002600"
     CDS             complement(566..1117)
                     /locus_tag="IWJ77_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP9454146.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(1291..1590)
                     /locus_tag="IWJ77_002601"
     CDS             complement(1291..1590)
                     /locus_tag="IWJ77_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP9454147.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(1617..1925)
                     /locus_tag="IWJ77_002602"
     CDS             complement(1617..1925)
                     /locus_tag="IWJ77_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9454148.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(2042..3001)
                     /locus_tag="IWJ77_002603"
     CDS             complement(2042..3001)
                     /locus_tag="IWJ77_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9454149.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            3145..3921
                     /locus_tag="IWJ77_002604"
     CDS             3145..3921
                     /locus_tag="IWJ77_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9454150.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(4019..>4649)
                     /locus_tag="IWJ77_002605"
     CDS             complement(4019..>4649)
                     /locus_tag="IWJ77_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9454151.1"
                     /translation="FDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKIGTTASI
                     FDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIKMAIASS
                     SPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEECLVIEDS
                     YNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
BASE COUNT         1445 a          850 c          701 g         1653 t
ORIGIN      
        1 gctatgagtc caactatgat taccccaact gtcttaatta cttttccttt atctatattc
       61 ggatgcgcaa gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca
      121 atcagcttag ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct
      181 tgcagagaaa ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat
      241 catgttttcg attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat
      301 ctgttgccct cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc
      361 ctcaaaaaag tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat
      421 ctacacaccc cctgttattt tttacataat gaaaatagca tattactggg tacattgata
      481 attcatataa catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt
      541 ataagtaaca taagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca
      601 attgccattc attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta
      661 tcaatacttt ggtcatcttt ttttgtttcg cactcaattg atcgtaaatg gtcttcgctt
      721 gttcataagc cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtgttga
      781 ttttcttttc tgtgaaaaca gttgttaata tttccaaaga ttgttcatca tttccacgag
      841 gaaatacccg aggacttttc gcttttgtat acgaaaaaag attcacagca ataaaaccag
      901 taaatcctaa ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg
      961 gttctgtatc tgttggacga atagtaatga ctaccgctaa tttatcttcc gcttcttttc
     1021 ctttgatcgc ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa
     1081 cttctgtttg tgtggtcact aattcttttt caatcatctc tttttctcct atgctaattt
     1141 ttgttcaagc aattcaatca ccattgccgt tataagtgtc aatactagtt gaaaatattt
     1201 catattcttt ctcctttttt cttcagaatt tgatgaatcg ctgtaaggat atcttctaaa
     1261 catttcgcaa tcattctcaa ataatcgatc ttattcattc gttcctccaa tctgactcca
     1321 aaccaatcgt ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt
     1381 aatgaaataa actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt
     1441 catcactaaa cgtggtagag taacatcttc aggaagcata cccatttcaa tttcctcttt
     1501 ttccatcgta acggattcat agccccactc ttcaggagta gtgactagct gtgcacactt
     1561 ttccagtact tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatatcag
     1621 tattcttctg gtaataacat cgtttgatac tcacccgaat caatgaccca tattttttga
     1681 gacactgatt tcaaacgaag cttaggtgaa atcagatact cttttcttgc ttctggttct
     1741 tcttgacgat gaaccaactt ttgatttcct ttttccgtag aaaattcaaa gatttgaaga
     1801 tagtctaaat gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt
     1861 aattccaccg gcaaactgtc cattactccc tttgtgacat aacgttgtcc tttattttga
     1921 aacatttctt ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg
     1981 gcttcatgga tataatatat atttgatatt caatttgatt cattttatac tatctgattt
     2041 gtcaaattaa gctagacaat atgtctttat ctatgcagct caaaaatact tccaatggtg
     2101 ttcgataatt taatgatttt cttggaatat ggtttctttt cgaggcaacg gattgaataa
     2161 aggtttcatc cgtgtcccta aagtccattg ttttaggtaa gccatctcgt ctaagcaagc
     2221 cgtttgagtg ctcatttagc cctctttgcg aaggtgttcc tggatctgcg aaaaagatgg
     2281 caatatcgtt cgagttactt atagatcgcc agttggaaaa ttcttttcca caatcaaatg
     2341 tcatggattt aaacaagtgt ttaggcatgg atttgaacca atcatttaac ctgttttcga
     2401 tatcttcggc tcgtctgcct gtcggtttca aagtaataat aaccttggat aaacactcaa
     2461 ccaatgtaat aacagcgctc ttgtgtttaa ctcccacaat ggtgtcgact tcgagatgac
     2521 caaattcttt agtcaggtgt ggatagagac tgttccgttc atgaatcgtt ctgcgaaagg
     2581 cctgttttcc acgcttttct tgatgattat tcggtttacg tttccccttc ataggtaacg
     2641 actgtgtgtc aaaaaggcct tttttaaaca tccgatagag tgtacgaaca gaacaatcaa
     2701 ttggaaactc gccacggccg ataatcacat cgggtgacca tccttgagct accttttctt
     2761 gaacatatgt tgattgttca tcagataaaa cggttgaacg tcgtccacaa cgagatttat
     2821 tttgtttgta ttgacggtaa taatctagca ctttttttcc ttgtttgaga aacgaataaa
     2881 cattataaat ggtttgtcta gagcgattta aactttgtgc gacaagagcg acaggtttct
     2941 cgatattgaa ataagactct atgattacta gttcgtctgt tgtaagatgg gtgtaagtca
     3001 tttgtactca ctccttatta attctttggt cggaactatt ttgagtgtag cacaaatgat
     3061 ctttttagtt gtctagctta attttacaat cgacgtttta ataaattgtg atataatgaa
     3121 gtataacttt ttagggagtg tactatgaat tttcaagata atagaccatt atatttacaa
     3181 gtagaagctt ctatacgtaa cgatattcgt gaaaaaaaat atcttccagg agagcaatta
     3241 cctacagaag aagaattatg taaaatttat ggagtaagta agattaccat tcgaaaagct
     3301 tttaaacttt tagcagaaag tggaacagtt gaacgattaa gggggaaagg gacctttgta
     3361 actcaaaaaa aagaaagttt aaaattagct ggagttcatg gatttagcga ttctctttct
     3421 acccgaggac ataaaattag atatactgtt ttacattcta aaattataaa ggctgatgag
     3481 tttttagcta ataaattaca aatcaatgta actgatagag tttacaatat taaacgttta
     3541 atgtgggaag acggtgcccc gatgggaatt gataattttt tcgcatcaga agaaaaattt
     3601 ccaactttat ttgaaaaagg tagccaatat acttcattat ataaacttct agaagatgac
     3661 tatgatgtca aagctacgag ctctattatg gaaataaacg gtttatctgc aacacctgag
     3721 ctttccgact tattgcaatg tatagtagga gatccgctat ttattttaga aaaggttggc
     3781 aaagataaaa ataatcaacc atttcattat tctcaatcca cagttcgttg tgatagaatt
     3841 agttatgtta ttgaagtcac agaaaatatc actttagata cggtgcataa aaatgattca
     3901 gatcctaaag taatagattg atattaaaag aaatcatacc caaagcaatc aattttgatt
     3961 agatttgggc atgattttta tttttactaa tattctctaa atacatcaaa aaacttattt
     4021 atattccaaa tttcacctgt acttcttgtc gcagatattt aatatcgtaa ataacatcat
     4081 ctgctagact tacatcttga gaaaaacgtt tatctttaat tgcaagaacc ttcatatttg
     4141 ctcttttacc agcttttata ccattataag aatcttctat caccagacat tcttcaactg
     4201 gaattctgag ctgttctgaa gctttaagga atatagtagg attaggttta ctttctggca
     4261 aatctcttcc gctaattaaa caatcaaaat atgactctat tttacaagtg gtaacaactt
     4321 catttatagt atccattgga ctggaagaag caattgccat ttttattttt ttctttttta
     4381 accattcaag ctgttcaatt agtatattag gaaaaatatc caagtaatca atcttttgcg
     4441 atttccaata tttagtaaat tttcccttca attctaaact gttacatccc tcattgtact
     4501 tttctatatc atcaaaaata cttgctgtag ttccaatctt tttttgataa gaatacaaag
     4561 gatagcttaa attttcttgt tttaaaaaat taaataccca cttttgatac ataatctcgc
     4621 tatcaattaa aactccgtcc atatcaaaa
//
LOCUS       DACDRK010000120         4634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDRK010000120 DACDRK010000000
VERSION     DACDRK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.123"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IWJ77_002606"
     CDS             174..518
                     /locus_tag="IWJ77_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9454152.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IWJ77_002607"
     CDS             519..938
                     /locus_tag="IWJ77_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9454153.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IWJ77_002608"
     CDS             complement(1030..1380)
                     /locus_tag="IWJ77_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454154.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IWJ77_002609"
     CDS             complement(1346..2566)
                     /locus_tag="IWJ77_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9454155.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="IWJ77_002610"
     CDS             2932..3498
                     /locus_tag="IWJ77_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9454156.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="IWJ77_002611"
     tRNA            3643..3730
                     /locus_tag="IWJ77_002611"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="IWJ77_002612"
     CDS             4064..>4634
                     /locus_tag="IWJ77_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9454157.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DACDRK010000121         4611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDRK010000121 DACDRK010000000
VERSION     DACDRK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.124"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(86..340)
                     /locus_tag="IWJ77_002613"
     CDS             complement(86..340)
                     /locus_tag="IWJ77_002613"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9454158.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="IWJ77_002614"
     CDS             complement(451..705)
                     /locus_tag="IWJ77_002614"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9454159.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="IWJ77_002615"
     CDS             complement(898..1173)
                     /locus_tag="IWJ77_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9454160.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="IWJ77_002616"
     CDS             complement(1145..2098)
                     /locus_tag="IWJ77_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9454161.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="IWJ77_002617"
     CDS             2710..4203
                     /locus_tag="IWJ77_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9454162.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="IWJ77_002618"
     CDS             4338..>4611
                     /locus_tag="IWJ77_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9454163.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DACDRK010000122         4475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDRK010000122 DACDRK010000000
VERSION     DACDRK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.125"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            323..1054
                     /locus_tag="IWJ77_002619"
     CDS             323..1054
                     /locus_tag="IWJ77_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9454164.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="IWJ77_002620"
     CDS             1199..3226
                     /locus_tag="IWJ77_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9454165.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="IWJ77_002621"
     CDS             3334..4431
                     /locus_tag="IWJ77_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9454166.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DACDRK010000123         4465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDRK010000123 DACDRK010000000
VERSION     DACDRK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.126"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWJ77_002622"
     CDS             574..1008
                     /locus_tag="IWJ77_002622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454167.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IWJ77_002623"
     CDS             2298..3221
                     /locus_tag="IWJ77_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9454168.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IWJ77_002624"
     CDS             3237..3776
                     /locus_tag="IWJ77_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9454169.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          674 c          757 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
      421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctaa
     4261 acggtcggcg aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DACDRK010000124         4465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDRK010000124 DACDRK010000000
VERSION     DACDRK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.127"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..851
                     /locus_tag="IWJ77_002625"
     CDS             66..851
                     /locus_tag="IWJ77_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP9454170.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1560..1919
                     /locus_tag="IWJ77_002626"
                     /pseudo
     CDS             1560..1919
                     /locus_tag="IWJ77_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2214..3347)
                     /locus_tag="IWJ77_002627"
     CDS             complement(2214..3347)
                     /locus_tag="IWJ77_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9454171.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(3338..4006)
                     /locus_tag="IWJ77_002628"
     CDS             complement(3338..4006)
                     /locus_tag="IWJ77_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9454172.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
BASE COUNT         1459 a          869 c          739 g         1398 t
ORIGIN      
        1 cccttttaga aaaagcaaaa aaaaaagaaa aaatcttatt cttgataaca agctacgtga
       61 cgtatatgcc taacaaaaat agaacttttg aatcaatctt aaatgaaggt ttaaccacag
      121 aaccggcatc cattattttt gatgaagtta tcgatgacgt tgaagatagt aagtatcata
      181 aaaatttaaa agaacttttt tattttgcac tcaacaatgg accaagtttg ttcactggta
      241 tatcagtacc tgatagtgat aatccattaa ttatggcaaa aatttactta acaaaatggt
      301 gtaataaata catcagggat aggaataacc cagcattaaa aaaacctctt aaaacatttg
      361 gcgaaaaaga tgctgcttta actactagag tagcagcaaa tgctaatatt gatgatcaaa
      421 aagtattgaa tgactacttg cgtggacatt ttttatatat gagcgctgag aacatgaacg
      481 gaagtatttt agaggaatat ttagccgaag tgttagaacc tgaaggttgg atttggtgcg
      541 ccggctctgt atatcgagct gttgatttct gttatttagg aacctcacca attcttttac
      601 aagtgaaaaa taaatacaat acagaaagca gttcgagttc agctataaga gtaggaacaa
      661 caattagaaa atggaatcgt ttaaataagt cgacaaaaat ttctggtttg gatagcccta
      721 tccccaattg gaaagcatta atagaaatga ctgaagctag taaggaactg gcagctaagt
      781 taactgaaaa tagttattta gcttatatta atgaaaaatc cacacgagaa ttatggacac
      841 tcgatgacta acaatatcct tattagtatc tatccacgat acatggaaaa aattataaac
      901 aaacaaaaaa actttgaatt tagaaatttc tatcttacct cagatcctta taatatcttt
      961 tggatttacg aaacaatccc caccaaagct ttaaagtata aattgattgt aaaaaatcca
     1021 attactaaac ttaagaaaaa tcaacactac tttttaggag acgatgagtt tgaacaatta
     1081 gtttaaaagg gaaaatatgc ttctatcatt acaagctcgt ttggtgtaag atgggtataa
     1141 gtcatttatg ttcactctcc ttgtatgctt tagcgggtat tacaatttga gtgtaacata
     1201 aatggctttt ttatttgtct cgcttaatta tacaaacggc gaattataaa taactcataa
     1261 aaaatatttt gactttcaaa atcacttttg ctaccagaag ttgaggcgaa gtgttttaag
     1321 catagttgta ccctagtttg accgttaaaa ataaataacg atcgctagag tgcaactatg
     1381 tttgtttgcc ctaccgtcag cgtcggcttg gcctctcaca tccattaccg tcgggattat
     1441 ctccgttaca tacccttaca aaatacgccc tgtaagcatt gcatgaaacc accgtgcatt
     1501 tgcaccgtat ttccgcccta gccgttcaaa tcggtcgcta ccaccattca gaaacacgtg
     1561 cggtaatgga aggtttggag gaagctatcc agaccactaa cgattgtccc tatcgtcgta
     1621 cgtttcactc agatcaaggt tggtcgtatc aaatgaaagc ttatagaaac aaattgaagg
     1681 agtacaaaat ctttcaaagc atgtctcgga aagggaactg cttagataat tcacttatgg
     1741 agaatttttt tggcttatta aaacaagaga tttttcacgg gaaagtttat aactgtttcg
     1801 aggaattaaa atcagccatc gatagctata tctattacta caataatgaa cggataaaac
     1861 agaaattaaa ctggcaaagt cccgtccaat ttcgtaaaac aaccgttacg gttgtgtaaa
     1921 aatatcattt tagagcatga aaaaaagagt ggatttctcc actccaaaaa agtctaactt
     1981 ttgggggtca ctacattttt gatccttttt atttgattaa ttatatccag ctggataata
     2041 attttggggg caacatttct atcttactat tatcttccta aagtagtaga ctttcttatt
     2101 gggaaaaatt acaacaaaaa ggagtggctt agttgacaac tcctttttgt ttacaaatca
     2161 attggatatc tataaattgt attttctaac gaattttata gacatctgat ttgtcaaatt
     2221 aagctagaca atatgtcttt atctatgcag ctcaaaaata cttccaatgg tgttcgataa
     2281 tttaatgatt ttcttggaat atggtttctt ttcgaggcaa cggattgaat aaaggtttca
     2341 tccgtgtccc taaagtccat tgttttaggt aagccatcct gcttgagccc atgcactcgg
     2401 tactgatgag aaaaaattga tgcctgtttc tcctgaggaa tgccaattcc ctgatctttt
     2461 acttcaataa tcacgctacc cttttggttt gaaatggtga caattaccag ttttccttca
     2521 ccatatttga ttgcattgtc aactaaattc gaaaccgccc gataaatcca acgatctcgt
     2581 ccatgaatca gggttggttg atcttcagta aacgcaaaca aaagattcgg ataattcatt
     2641 ttgtaggcat cacaaacttc cgcacagatc agtgaaacgt ctacgacttc cgtgctaaga
     2701 tcctctgagt tattggataa tgtcaaaata tcgtcaatac tgtcggtcat ccgatctaaa
     2761 agcattaaat ttccttgatc ggcatgttgc cgttctattt ccagattcgt acgcaaaata
     2821 gcgatggcat ttttctgctc gtgggtcaga taactattta atcgtcgata atcctccaaa
     2881 tgaccatcga acttatcctt tagcttatta aaagccgcat ttaatgcctc gtccttcaca
     2941 gtatttttag gaacttgatc aataaacgtt aaatcacgcg taatcgctaa gatttgatta
     3001 ctgtaaattt tgcgtaaaac caaccaaaga ataaacgtta ataacatgac ggcagaacaa
     3061 ataaccagca ccatcgctgg taaatattta aatagttcgt cctggtaagc tttatctatc
     3121 atcgaaatag tcataaattg attgttggaa gatttcgaag cttccatctg cgtctctgct
     3181 tgaaccgtct taccctcttc cataaaataa acatcttcac ctaaggtaga tgcgatactg
     3241 attccctcca aattatttct cagaaagaag aaactcacta aaatcacgcc tgtcaccaac
     3301 agtgaaaaag ccagcatcgt taacgtcatt ttaaaattta atagcatagc tggtacccct
     3361 ttgctcgaat ggtttttaag acctcttttt cagctgcttc agccagtttc ttttttagac
     3421 gagccagatg cactcgcaga accgacgaaa aaggatcaaa atcttcatcg tagacatgtt
     3481 cagaaatttc ctcagacgaa acaaccgctg gatgacgctg agcgatgcac agtaagatat
     3541 caaattcctt gggtttcagg accacctctg tcttttgata gctgaccctt cgacctaatg
     3601 gatcaatctt caaagcacca attgaaatct gcggcagcgc acgtccatga taacgacgaa
     3661 taaccgcgcg tattcgtgct tttaattcta gtaactcaaa gggtttgact aaataatcat
     3721 ctgctccaaa atccaagcct tttgctcgtt cttcaatctc atcacgtgca gtaataatga
     3781 taactggact ttcgacttct tcagcacgga gactttgtaa aacagataat ccatcttttt
     3841 taggcagatt taaatctagt agtatcccat cgtatgaatt gatgaaggcc ttctcttctc
     3901 ctgactcgcc atcataagct acatctacag aaatattctc ctgctctagg ccctttttta
     3961 cggataaaac cagctcacga ttgtcctcaa ccaccagtac acgcatgtat attcactcct
     4021 catacggatt tagtaattac acttatgctt tttagcatag tacacgctct tcttcaaagt
     4081 ccacgttctt tttgtgaaga tttgaagaaa ttcatacatt ctgttaaaaa acacataatt
     4141 aagaatgatt ttactgtttg aaaataccta gaatagaaaa agactggaac ctttcctcca
     4201 gtctcactac tttctatttg cttggttttc ttgtatatac ttttcaaagt tcgttggttt
     4261 atgaccaata agcctcgtta gaatactgaa aattgccatc aagtcattaa gtaccacatg
     4321 taagaaaatc tctctaaaga aaaacccctt cgttttaact cacttactat tttagaattc
     4381 gttcccctaa taatgcagca ggttctgttg cgaagtttga aaatcaaacg cgccctctct
     4441 gaactccaaa tatcttaggc tgtta
//
LOCUS       DACDRK010000125         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDRK010000125 DACDRK010000000
VERSION     DACDRK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.128"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWJ77_002629"
     CDS             complement(543..2048)
                     /locus_tag="IWJ77_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9454173.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWJ77_002630"
     CDS             complement(2045..2731)
                     /locus_tag="IWJ77_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9454174.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWJ77_002631"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWJ77_002631"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9454175.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACDRK010000126         4424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDRK010000126 DACDRK010000000
VERSION     DACDRK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.129"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWJ77_002632"
     CDS             690..1658
                     /locus_tag="IWJ77_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9454176.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWJ77_002633"
     CDS             1669..2484
                     /locus_tag="IWJ77_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9454177.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWJ77_002634"
     CDS             2552..3262
                     /locus_tag="IWJ77_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9454178.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4424
                     /locus_tag="IWJ77_002635"
     CDS             3298..>4424
                     /locus_tag="IWJ77_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9454179.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1490 a          661 c          921 g         1352 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaa
//
LOCUS       DACDRK010000127         4328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDRK010000127 DACDRK010000000
VERSION     DACDRK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.130"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IWJ77_002636"
     CDS             complement(<1..281)
                     /locus_tag="IWJ77_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9454180.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2136)
                     /locus_tag="IWJ77_002637"
     CDS             complement(730..2136)
                     /locus_tag="IWJ77_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9454181.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(2359..2721)
                     /locus_tag="IWJ77_002638"
     CDS             complement(2359..2721)
                     /locus_tag="IWJ77_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454182.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="IWJ77_002639"
     CDS             complement(2725..3033)
                     /locus_tag="IWJ77_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454183.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="IWJ77_002640"
     CDS             complement(3042..3461)
                     /locus_tag="IWJ77_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454184.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNSGFDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="IWJ77_002641"
     CDS             complement(3454..3687)
                     /locus_tag="IWJ77_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454185.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(3684..>4328)
                     /locus_tag="IWJ77_002642"
     CDS             complement(3684..>4328)
                     /locus_tag="IWJ77_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9454186.1"
                     /translation="FNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFI
                     TQYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTW
                     WRLELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLA
                     KNTKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1222 a          966 c          614 g         1526 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaaccat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc gctctctcag aagccatcag agccttatca cgctcgtttt cttctctttg
     1321 cctataaata tacctcaact catcagaaac gctctcacgc tcttttacag ccgtttggcg
     1381 caattcagaa atttcactca tgatttcttt gtattcgtga atatctttaa ttttgttggt
     1441 accgacaata tcccttttcc aaccgtaatc attcattaat cgttctatag agtcaacttc
     1501 ttgatttcta aaattattga aaagcactcg accattatct ttatcagcgt gtatcccttg
     1561 ttgaaccagt gctttattaa agctcggctg cttttgaaca ccccgtttat aaccttcagc
     1621 aaccggcaca atattcaagt gcaggtgcgg acttatttcg tcattatgaa taacagcatt
     1681 ataaacagcc aaattagggt ttctctcttg aaactccttg taatactgaa ccaaaatttt
     1741 attggcaatc actcgatttt gttcagcttg ttgtttcgat atgccagtag aagaaccatc
     1801 tgctcgattt gtctggaaat catctaaaat gcctatttgc acaataaatt ctcgttgagg
     1861 ttccaacttt ttatccttgc gcactttcgc taaatagtcc tcgatttttc tatcggctct
     1921 tttttgtttc gcattatatt tttctaccac ttcgccaaac aattcctcat atttatctgc
     1981 tagattcgtc tgctctaaat aaatattttc atccgatttc gtgaagtcaa tgtgcttatg
     2041 ccaatcattg gttttctggc gatcagttaa ctcacgatta ttatgagaaa tacttgtatt
     2101 cttcgttcct tttttaaatg aaatactaaa ccccaagtta aaaccctcct tcgcgctgtc
     2161 gctaataaaa agattgtaaa cctttctgtt actctttgtc aaagagtaaa cattttccta
     2221 attgtgacat ggatgtcaca attccaaacg ctctccgagg gttttcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtatcac gctatcaact ccttttatgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagtcgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgtttt
     2521 ttcgtgttgc tgatcgtcag aaattgacga taaccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttgcgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctag
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcaaagtca atttttgttg gtcacgaatc atttccgtta attcagaaat atgcctatct
     3181 ttttccgcta attgttcacg caaaaattca ttttcttttt ctaacgggaa atcattgcct
     3241 gcatcattga ccggtaaatc accagtcaat tgaccacctt ttgaccgata aattgaccgc
     3301 acaacaccaa tcaattcctt gtctaacatg ataaccccat tatctttata ccgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tagatatacc caactcatca
     3421 gcaaggtttt ttatggtcat tttttctggc aaatcagtca ttatcttctc cgttactttc
     3481 agctacccca taaacatatt taacagcatg cttagggata atgattgggg aatcaatttt
     3541 attatttttc aaaatcaact catactgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggattgaa
//
LOCUS       DACDRK010000128         4310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDRK010000128 DACDRK010000000
VERSION     DACDRK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.131"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IWJ77_002643"
     CDS             complement(65..1285)
                     /locus_tag="IWJ77_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9454187.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IWJ77_002644"
     CDS             complement(1305..1910)
                     /locus_tag="IWJ77_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9454188.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IWJ77_002645"
     CDS             complement(1959..2891)
                     /locus_tag="IWJ77_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9454189.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="IWJ77_002646"
     CDS             complement(3312..4172)
                     /locus_tag="IWJ77_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9454190.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DACDRK010000129         4226 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDRK010000129 DACDRK010000000
VERSION     DACDRK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.132"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(249..791)
                     /locus_tag="IWJ77_002647"
     CDS             complement(249..791)
                     /locus_tag="IWJ77_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9454191.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            926..1315
                     /locus_tag="IWJ77_002648"
     CDS             926..1315
                     /locus_tag="IWJ77_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454192.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1319..2083
                     /locus_tag="IWJ77_002649"
     CDS             1319..2083
                     /locus_tag="IWJ77_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9454193.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2212..3159
                     /locus_tag="IWJ77_002650"
     CDS             2212..3159
                     /locus_tag="IWJ77_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9454194.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3289..4029
                     /locus_tag="IWJ77_002651"
     CDS             3289..4029
                     /locus_tag="IWJ77_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454195.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1428 a          704 c          843 g         1251 t
ORIGIN      
        1 gaggtcttaa ggtatgaata acatagaaaa aataagatcg tcaaagttgc aaaaaaagag
       61 cctaaatgta aaaaattagt ggttctacgt tgaatttcca taatatctcc aaattaaatt
      121 gataagtata taaagtataa taacaaattg atgagtatat gtcgatgttt tttaatcaaa
      181 ataataaata aaaactgtct aacatttaaa atttaattta gatattagac agtttttaag
      241 tttgtgagtt attttccttg tcgttttctc acttttttag tccagaaacc acctgaaata
      301 tattttggct cataagaaat gataaaagca cgctcttcga tttctttgat caggcgatac
      361 agttcacgtt cgctttttct aggagaaagt atttcgagaa ctagccgctc accttccagc
      421 cccattgcag aagtctgtgt cacaccataa ccgtgttctc tcagtatacc cggcaaactt
      481 ttatcttctg cgctatttgc tggcaaaatc gttgtgacca tgatgtatcc taatgccaat
      541 ttgtcttcaa tcaaaattcc gacactgata cctactgcgt aacctaacgc atagacaaat
      601 aaattcagtg gattatctaa acgatctaaa acaagggata agccgacgat ataaatcgtg
      661 atctcgacca tactgacaag tggagcaatc agtcgatagc ctttcattgt caacatgaaa
      721 cgtaacgtgt tcagagtgat ataagcaaaa ttaatgagga agatcatccc caacattttt
      781 ggatcaatca tttgtaaaac tcctttcatt tatataacaa caatatcaaa aaaaatcgta
      841 agtgaagaaa aattgaaact ttatacatac aaaaaaatga taaaatgatt ataatagaat
      901 tataaagata ggttaggtga ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca
      961 cgtgctaagt ctccttctca gtatatactt aggtgtctat tcgattcaac agccaattga
     1021 ggtagagaac attgtttttc agatgtcgct gacagatggt ttaattttaa gcgttttttt
     1081 ctttttgata ctgtttattg gtaatatttt cggtagcgtt gccagttttt tgaatttttc
     1141 tatttatcca ctcctttcgc tagcattagg agtggtggga ctttgttctc tgtgcttatt
     1201 tcccgagcca ttcagtcctg catttatatt atttggtaca ctgaacctgt tccagacaac
     1261 tgttggagca tggttgctat ggcgatcagg aaatttaatg aaaataggtg agtaacaatt
     1321 gaatccaatt atattgaaac gttctgtcca tcatattcca gtgttagaag ttgttctgaa
     1381 agaattaaga gaggaacgta ttcccgttgt catctattat cacggatggc agacaagtaa
     1441 ggagttagtt ttgacgcaag gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc
     1501 agatgcgatg aatcatgggg agcgcaaaca gccggtttct tctattccat cctatacatt
     1561 ttgggatagt atctacggaa atctgtttga atttgacaca cttatcaagc atttagaaaa
     1621 aagaggtctt ctacatgatc agcttgcagt tggcggggtt tcgatggggg gaatgacaac
     1681 atgcgccttg ttggctaaac atcctcatat agtagcgggc gtgtgtttga tgggttcccc
     1741 tgcacctttg gaatatggaa aagaaatcgt aaggcgcgca tcggaatata attatgtcgt
     1801 accaaaagac tattttgatt tgatttcatg ggttaagagc tatgatttgt cattgcagcc
     1861 ggaaaagtta gctggacgtc ctctgttctt ttggcatgga caagctgacg aaaaaatccc
     1921 ttatatacaa acggaacgat ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat
     1981 tgtttttcgt tcctcaaaaa cacagcgaca tatggtacaa ataccactaa tggaagaagc
     2041 ttccactttt ttagcaaaaa atttgataaa aaagtataaa taattgaaac ggaaaatcac
     2101 tcatgacgat agaggatttt ccgtttttta ttttgggcta taataaactc tgttaggaaa
     2161 atttggtata attgatagta agaatgacaa catgttagaa aggaagagag catgcccact
     2221 ttagcagatg ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat
     2281 catccggaac aggtgactga cgaattgaaa gagttagttt tctcagcaat ggctgaactg
     2341 gattaccgtc caaatattgc tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa
     2401 ttatttatac tggaagaaat cgacacaact gagccctatt acatgaattt gctgatggga
     2461 atcgctaaac ggatcggtaa agcgcattat tctctccaat tagttacaaa tgatgccttc
     2521 gacgtcggtt cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga cttcgattgg
     2581 atcgaccgtt tggaaaaacc agttgtcttg tttggggaaa attctgaagg atatgattat
     2641 gtagacagcg ataatgaata tggtacagca aaagcaacgc aatatgcctt agaaagagat
     2701 tatcaaacga ttatttatat cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa
     2761 actggttatt tatctgtgat gaaggaagtt gatcttccta gtaaaatctt gcggtttcca
     2821 aatcattcga cagaagtaga aagatatatg acagaaaact gggcagattt tacagataaa
     2881 acttgttttg tatgtagttc agatcggcta gcactaggag cagtaagagg gattataaat
     2941 gctggaggac acttgcccga agaatttggt gtgattggat ttgatggggt atttttagac
     3001 caaatcagtt cacctaaatt gacaacgatc aagcaagata ttatacgtat gggtgaagta
     3061 tgtggagaaa tgttgttaaa aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt
     3121 ttttttcctg aactagttat cagagagaca acgaattaag tcttattact atatatatat
     3181 ttatgaaatt catcgctatt ttcttaaaat aatcagcgca ttgctactga aaacaatgaa
     3241 tctgtgctat tattgagaaa agttccattt ttgagaagga tggtttgaat gaaagaaatc
     3301 agacgaaaac atttccgttt tcctgcgtac gatgatgagt taggcgtaaa attaaccaac
     3361 gacaagaagc gagtattatt tgacggacaa gaagatcttt tgactgaaca gaatcagtcc
     3421 ccgtttgaac agatgaaaga atttcatttc acgaacacga acaccgagaa aatccgtcgc
     3481 aagcgtcgtg caacaaatgg attgaagtca gctccgcgta aggaaaattt ggaaaagcat
     3541 aaaggaaaat taccggatta taccggccat taccaatcag aaatcacatc gacaggacga
     3601 aaaaaacttt ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc
     3661 aagcatacta cacgaaacgc atctgttgac acgaaggtgc gggagcgttc ttattttgtt
     3721 cctaaatatg tacccgcatc gattatccca gaccctagag aaagcaaaat ttctgaggat
     3781 catttaatcg aatcgatgaa aaaaacggat gactcttatc tgttgttcga tacagaaccg
     3841 acgccgtatc aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa
     3901 acgttagagg aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta
     3961 gaacgctcat tacaaggatt gatcgatgat caaggaaact ctttgggaga aaatagctat
     4021 tttaaataag tgatatacaa atttatattc ggctacagtt ggaaaggaaa cgctgcggcg
     4081 tttccttatt ttatactcta tccgaaagtt tatatcgtat cctcaaattt ttttgagttt
     4141 aagcgaattt tttctcccaa taagtaagaa aataatggca aaataaaaaa acaaactatt
     4201 ttcgcagatc gtttgcaaaa atagtt
//
LOCUS       DACDRK010000130         4153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDRK010000130 DACDRK010000000
VERSION     DACDRK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.133"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWJ77_002652"
     CDS             <1..106
                     /locus_tag="IWJ77_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9454196.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IWJ77_002653"
     CDS             970..1182
                     /locus_tag="IWJ77_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454197.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="IWJ77_002654"
     CDS             complement(1460..3259)
                     /locus_tag="IWJ77_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9454198.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="IWJ77_002655"
     CDS             complement(3383..3979)
                     /locus_tag="IWJ77_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9454199.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DACDRK010000131         4073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDRK010000131 DACDRK010000000
VERSION     DACDRK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.134"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(58..384)
                     /locus_tag="IWJ77_002656"
     CDS             complement(58..384)
                     /locus_tag="IWJ77_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454200.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(371..1159)
                     /locus_tag="IWJ77_002657"
     CDS             complement(371..1159)
                     /locus_tag="IWJ77_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9454201.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(1527..1817)
                     /locus_tag="IWJ77_002658"
     CDS             complement(1527..1817)
                     /locus_tag="IWJ77_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9454202.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(1953..2132)
                     /locus_tag="IWJ77_002659"
     CDS             complement(1953..2132)
                     /locus_tag="IWJ77_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454203.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(2129..2479)
                     /locus_tag="IWJ77_002660"
     CDS             complement(2129..2479)
                     /locus_tag="IWJ77_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454204.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(2472..3797)
                     /locus_tag="IWJ77_002661"
     CDS             complement(2472..3797)
                     /locus_tag="IWJ77_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9454205.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
BASE COUNT         1215 a          755 c          593 g         1510 t
ORIGIN      
        1 actgattttt ataaaaaaaa tatcacgata ttatgatatc gtgatattga atgtaaatta
       61 aattttattt tgtttctttg ctagtttata tccttcaatc aagtattctt caggtatgga
      121 ttttaatagc gcatcttcta taacgtctgg tttacttttg tctgattcag caatagtcgt
      181 ccaatatttg agtgctgaca ttagaattgg atttatacga atgctatctg ctgttcttac
      241 actacttcct tgatctttta tgactttact tatatttttc ttggttaatt cgatttcttc
      301 tacttctttt gatttaaaat tctttttatt tttatttcct agtaatctac ttactgcagc
      361 ttgactgcga ttatcttctg ccatcaaaat caccaacttc ttttaaatat tcttctgtaa
      421 atgctttata gtcatcttca gctgttgagg gtttattaaa attgattaga tctgatccag
      481 cagcttgcgc ttcgtcaacg gttattgtcc tacgaatata agttttgaaa atttttgcac
      541 caatgactct agaaatttcg gttgctgcag acgtcatttc tttagaaatg ttataacgct
      601 tattatactt agtaaataga actcctcgta cttcaagctt gtcgttcgta tattctttta
      661 ccgtttcaat agtttcactt aactttatta gtccttttaa tgagaaatac gcagcttcaa
      721 ctggaatgat aatttcatca gaaacagtaa atgcgttgac tgtaagcatt cctaaacttg
      781 gtggacaatc aattataata taatcatagt tgtccttgat tggttgaagt actcttttaa
      841 gcctgtgttc acttcctact tgagtaaatt ccctctctgc agaagaaagg gctatgtcgg
      901 ctggcaaaac atcaattcct ttatagtcca gaatagcatc tgtaatgtca gcctcccctt
      961 ttaatacttc ataaattgta ggttggtttg ttccatctat atcgaaacct acattatctg
     1021 tagcatttcc ctgaggatca atgtccacta aaagtacatt tttaccgtaa acaaatttca
     1081 aacatgctgc taaattaacg ctagtcgtag ttttagaaac tccacctttt tgattggcta
     1141 ctgacaatat tcttgccatt aaaatatccc ccattcatct tatattataa tataattata
     1201 tcataatatt gtgatattgc aatatcgtat atgcattata ttgtaatatt gtgtgattat
     1261 tttgttttaa agatatatat cggttcggat ataaagatta ttcatatgtt attcgaaagt
     1321 aaaataatca ctttttatta ctttaaaatt ttttattaat cataagaaat atgttttgcg
     1381 agcagcaact agaataattt aaacattatt aaactgtcta tattgttact aataattccc
     1441 ttcttatagt gcattttctt agaaaaaaac aatattacga tatcatgata ttaagatatc
     1501 gtaatattat acaattgtga ttgatattat atatgttttt ttattttttt atcatgttta
     1561 atcttgaagg aatcatattc tttttctaaa gacaggagct tttcataaac atcagacatg
     1621 tttaaactag aggccaggct taaatcataa acgaatgaag caggtaatga ttttattcta
     1681 cgttgtcggg ttatccagct tgcaaccgtt ccttgtgggt aggcatataa ctcacaaaat
     1741 tgttctacag tgattcctaa attcttgata atgtaaacat tgatcgggtg cgagtaaaca
     1801 aatgtattga tagacatggt gcgtaagcct actttctttt attattgcgt ttgcaataat
     1861 agtttaacac acgcattatt ctagtatcta ctcctatttg taaaaacaaa aaaggattca
     1921 tagagataac tctatagaat ccgtaaaata ccttatatat ataacgggaa aatataaaac
     1981 ctaaaattat gataacctgc tgcagcttct acatagaaag agaagcgctc aacaccaacc
     2041 tcatttatca aacgttggcc ataaccccaa atctcttctg atagctgatc aaaggttata
     2101 tactttagct catattgctg gataatgttc attctaatcg actccattta ttatcctggt
     2161 aaaattcgat atgacgtatt tcgtcgtagt gaattttgtc ttttccaacg tagatcccca
     2221 gctcatcact acctaagatc ttgccaacaa tatcttctgg atagtatcct tcagcgtttc
     2281 gttcttcttt ttgaatagat actgatctat tttttatgac tgcagcgttc aatacttccg
     2341 caatctcttc agtcgtcatt tgttcttttg ctaaattttg actggttcgt tgttcattgt
     2401 cggcatcaat ttcttcagta tgatcagata aataaaaccc tgcccacttt aaacgtccac
     2461 gatcattgta atcaatcatt tttaattccc ccatttccgc ccgcatgacc accaacaagt
     2521 gtgcttctag taatgcttct agcgctttct aacattgaag aagcatgtac aatcgacgta
     2581 aaaccatatt tttgccgaat cttgtcaata attttatcta aattttcttc gttaatttgc
     2641 ttttgtggat cactaaataa atccagctga agcgaatcgg tgtacacaag cttgctatag
     2701 gttattccaa cgtgccttac ttcttgtcct tcataatact tatcaaacaa gaaaagacaa
     2761 taattctgca attcctttgt attatctgtg ataggtattt tcatctggtg tgaaaatcct
     2821 ggtctcgttt ctaagatcga cgtaccaatg tttaaatgaa cacatcctgt tttacaatta
     2881 tgctgcctga tacgaatggc tacttgttcc gccatttctt ttaccaccag ctcgatttca
     2941 tttcttctag tataatctcg tggtaacact tgtgaattac cataagattt ttcctttgtc
     3001 ggttctggcg ggattgctat atctgttcga tcgattccgt tagcatggaa gtacagctgt
     3061 aatccaatca cacctaatct attcttaatc gtataaggat tccagttagc aaggtctcta
     3121 atgctcaaaa ttcccatttg gttgagcctt tttttcattc gagaaccaat tccccaaaag
     3181 tccgtcattt cgggaatatt ccatactttc tcaggaacgt tttcgtacgt ccattccgca
     3241 ataaatcctt cattatgctt tgcttcattg tctaaggcta atttagccaa aaggggatta
     3301 tcgccgacgc ctaccgtagc aatcaaacct aactcttctt taatacgttc ttggatcatt
     3361 tgagcgagct tcttccttcg ctgacttcgt gtttcttcag tcgtaaaaag attcagtgat
     3421 tttgtcactt ttaaaatcga ttcatcgatc gagtaaatca gtagatcttc atcagccaca
     3481 tatcttctga aaatattatt tacctgcata tttcgcttga tatataggtt catacgtggt
     3541 ggaacaacgt gtagtgtttt aggaaatggt tgtggtaagt cacgtggtct actcacattt
     3601 gtaataccat accgcttttt tgcttcagga gaagaagcta atatcaatcc tgaaccagta
     3661 ttatcagatc gactcataac aacaagttct gttgttaatg gatctaaatt tctttctata
     3721 cattcgacac ttgcgtaaaa gcttttcata tcgattaaaa agtaatcatt tactggctct
     3781 tttgaataat ccatcataaa taacactcca ttcttccaca ttatacaaac atatgttcga
     3841 tgttgtaaag aagaaatagc taaatacata aaaaaagaag cataggtatg ctatcaccct
     3901 gcttcttctt atgatttgta tgatcaattt attctatcac tttactaatt aatttgaaaa
     3961 tctaataaaa actctcactc taaaagagtg agagactgca aattagagta cggacttttt
     4021 tggagttccg ttctttaata ttaacaaatg aataaaatcc ttacaacatt tga
//
LOCUS       DACDRK010000132         3998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDRK010000132 DACDRK010000000
VERSION     DACDRK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.135"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            749..1789
                     /locus_tag="IWJ77_002662"
     CDS             749..1789
                     /locus_tag="IWJ77_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9454206.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2613..2945
                     /locus_tag="IWJ77_002663"
     CDS             2613..2945
                     /locus_tag="IWJ77_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9454207.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3564..3905
                     /locus_tag="IWJ77_002664"
     CDS             3564..3905
                     /locus_tag="IWJ77_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9454208.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1463 a          510 c          720 g         1305 t
ORIGIN      
        1 ttttatgact tgtacaaatt tatctgatat tctcttatta agaactacct aatttgggta
       61 gttcttattt tttacatttt aagtagtatt taattgcaat ttggtagtat ttttttatta
      121 tcttattctt agaacggtag tacttaatat aaatataggt agtatctaat ttaagtatta
      181 atggtagtat ctaaattaac aggtagtatg taactacttt aaagtaattt ttttcagatt
      241 aacacagtag tatcttatta aataagttaa ttttaatggt agtacataat tattatatat
      301 atctgacatt ggtagtactt attgtatttt ttagtagtat ataatctatt gttttctagc
      361 ctaaagcctt gtcttataag aataatataa tatatactac tgaaataagg tagtatatat
      421 tactagtaat ggtagtatgt tttatatgca taaggtagtt cttaatttat tttttgtttt
      481 gacactggta gtacttaatt atctttaatt ttaaaactaa attttattca tacgtttttc
      541 tggtagtatt taattataaa taatatttat ttaaagtagt tcttaatgac tttaaaggta
      601 gtacataatt cattttattt ctgattttag gtagtattta attaaaaaac agttttaatg
      661 cgtttgcatt ttttatttat ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat
      721 aaaacaacgt gaaagtagag gatctacaat gactgatttt aaatttttta aggcggatag
      781 agtttacaac gaattatttt atcaatttcc aaaagtcttt attgtttctg acgaatacaa
      841 aaaaatgaaa gattcaacta agattgccta tatgctttta aaagcaagat tagagatcgc
      901 aatcagcaaa cggcaaatcg atgaagaagg taatgtttat tttacttata cgacaaatga
      961 actatgtaga gtattaaact gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga
     1021 atcctttggt ttattattac aaaagcagat gggatttaac aaacagttag ggaaaaataa
     1081 tcctaataga ctatatctag cagaattaaa agtctcagaa aatgatatct acttactcga
     1141 aaaatttgat agagagaata gggaaaacgt tgataaatca gagggtatga aaatcatacc
     1201 caccctcgac gaaaaatcag acgctgaatc ccttggggct caagagggta tgaaaatcat
     1261 accgtgccaa aacgttgata aatcagaggg tatgaaaatc ataccagaac ttaataataa
     1321 tatattagac actaatagac acaatataga cactgaaaaa gaccgcctac aagatcaatt
     1381 gttgttagac aattttgaga caattatgac aaacgacagc attgctacgt ttgtccctga
     1441 acgagtatta aatttgataa aaacattttc ttcaagttac agtgaagctc aaaaaaccgt
     1501 ccagactatt cataatgcaa agaaaaaagc tgaaatagaa agtggtattt cgatagtttt
     1561 tgaagaactc gatagttatt atgtcaatgc agaacaagaa ttatacacga cactgttaaa
     1621 agcctatcaa aaattaaaaa ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta
     1681 tgtaaaaaat tggtttatcg aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt
     1741 gaattatgaa agctccccaa gcactattac gaaagactgg ttagagtgaa actttactga
     1801 caaagtgatt gttgcttcat ggagtaacat taaaattaaa aattttttat tattctaatt
     1861 atattacgaa tattacaaaa taatagctat tttaatagaa tttataagtt aaaatattat
     1921 gataaaaatc acaaacgtag ccttttacgt cataagtgtt aaaagaaata attaatttca
     1981 aatatagaaa agggaacgta tgtttgcata ctcctttttt ttgagttata ataatacgcg
     2041 aagagagata atttcattat ttaaacaagc taaaaatctt gatattactt cttttcaggc
     2101 aatacgaata ttattcgctt acattttttg tataaattta attgtctgct atgcttgtga
     2161 tgaattgatt ttcgttctgc acttttggat gataaatttg gacaattgaa aatacaaaaa
     2221 aaagaaaggc ggtatgaact cggaaggctg gtaaccttcg tcagcgttca tacacggcat
     2281 taagatttgg cccttaatgc gttaaccttt cagtgttacg gtattacccg caatggattt
     2341 agtataacag atattcaaaa gtgataccac tttttttggc tgtgttaagg ttaaaaatac
     2401 gtgatattac gatgaatcta tgcctaagca caatgttata aaaatgtccg tgtggctctt
     2461 tgccgtatga cgaacgctga aattagaggg aacgcatgtt tggatgcgtt ccctcttttt
     2521 tgtttgtcat gaacgaaaat caattctcta tttttgaaaa ataacaagaa aaacttgtta
     2581 aaaatattta aagaaattgg gtggtttatt ttatgtcata tacaggaatt ttgagtctca
     2641 aagatatttg tcactacggt aaacggtgta ccgctacaga gaaaatcacg aagaaactat
     2701 caactggcca aagtaaaacc gttgtgcagt gtaagaaata tataatccag aaagacaaag
     2761 tctcagagga aatgatttat tacattggga aacaaaagca aataatttta aaagatccta
     2821 tccccttaaa agagctatat ccaacaatca aacatgttta tgaccaaaac ggtgtactga
     2881 ttggtcgaag aaaaaatgga gtcttgcgtt gtaccgcaaa agggatgggg cgtttgatcg
     2941 gctgaattga tcattctcat aacttgtatt ttatcgtttt tcgcttgctt gaaaatattg
     3001 tcgagacttc taaaggaact tagagtcact agaaaaataa ggagtagact tgatctatca
     3061 acaattccct tcggggagtg acggggtagg ttttaacgat tggtaacgta aaaaattggt
     3121 caatcgagta aatgcgcttg tttggatagt ggtgaaatga atttgtgtaa ataataagcg
     3181 cagagtaaac aagagggaat ttagaagtta gacaagttag aatagcgtct agattaatca
     3241 tgagcagcta ctaagggatc tagactttga cgcaaactca tggaataagg agtaggtctt
     3301 ctcgtaatgg ggaggacgca caagaaattg tgtcgtgtgc tgcagtacat gtagatactt
     3361 gcgaggacgt tgattattgg atcaatcgag catttaccgc tgtctactag ggtttctcgt
     3421 ttcggacgcc ataaacgagt cgaaattttt tatgtatttt taagtaaacg aaaaacgata
     3481 aaatatgaac aagtcctgaa taaagtgaaa aaattgatat aaaacataga gaaaatgaaa
     3541 tttacgtaat ggaggaatga caaatgaaaa attatgatcc gaatatacga ttgggcacac
     3601 atactattaa ggtttctttc caacgttggg attacaaagg atttgtgacg tttagaagag
     3661 gcggaaattg taaaggatta gatgttttag cattagacga ggatgattta tacgatcaaa
     3721 cattgactga taatccaatt ggattcggct tgctacctga agatgatgaa ggtaatgaat
     3781 ggtttaaaat gacgcttatg aatgataatg gagacgaatt atctgttgaa gatacttggt
     3841 catatttgag tgattatatt gtcagctttg aaatcattga atttgtagcg gataaagaag
     3901 aataaaaact ggaggaataa attttacaga taaaggctta gaaggagtca cgatgattaa
     3961 taaaaaggta gtattacctt aggcattgag taaggaat
//
LOCUS       DACDRK010000133         3830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDRK010000133 DACDRK010000000
VERSION     DACDRK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.136"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(615..839)
                     /locus_tag="IWJ77_002665"
     CDS             complement(615..839)
                     /locus_tag="IWJ77_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454209.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            1039..3807
                     /locus_tag="IWJ77_002666"
     CDS             1039..3807
                     /locus_tag="IWJ77_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9454210.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
BASE COUNT         1328 a          645 c          724 g         1133 t
ORIGIN      
        1 aaatgagaaa acatgccagt ctcttcattc ataggaacgt ctattttact taatttttct
       61 ataaatgaat tctgcataaa aaatatatca agtaaatgtt tatagatgaa actctattta
      121 cttctttttt aatttgttct tgcaatcttt tttgctacgt aatttttgta aaaaagtggg
      181 ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa aatgagaaaa atatttattc
      241 gtttattgtg ggtactatct tagaggaata aataatagtg gagaaaacta tggtttggcg
      301 cagattctat caggtttgat ggaacgaaga caacctcccg acatcaagtc aaaagctact
      361 tgagattttg atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat gtttctacct
      421 tgttttcgta atcaatggga aaaaactaag agtggtgtac gtaatcaata aaaaggatgg
      481 tgacaacagt acctctttac tctctgaaag catcataact cgtttttcct cattatgcca
      541 ataaattgtt gttataccac atataaggat ttgtaatagc taaaaacaga caggaaagtt
      601 gaaatctgtg aatcttattt tgtatggaag gatttataga acgcaaaaat caagataaag
      661 atgaataaaa atcctgtttc ggctatatat tgcgtatcta cgccaataaa acgagtttgt
      721 tctgtcatag ccatataaag caaaggcaaa aatacaacta cagcgagata agatccgatg
      781 atttctcgaa aagttttcca ggaagtatgt ttcctgtttt tatatttatc cgatttcatt
      841 gaatagtcat agcctcctta ttctttcata tgttgaaatt ccagtataaa aactggaagg
      901 aactgtacgt tttttagaat aaaaattata agggataagt aggctgctgt ctataataag
      961 atatattttt caattcttta ttaaagattt tattgacaaa atgaatccgt tttctatgat
     1021 ggaggagggg tgaataagtt gttgaagaag gaaattttat gttacttaga gaaccagaca
     1081 gcctttttcg atttagaaca tatgaacgag gtctttacag cacaaaggtt agctgatcat
     1141 tttggggtaa aaagaaacac agtcagtcat tatttgaacc agttaaatga tgagcaacta
     1201 ctagtcaaaa tcaattctcg accagtgatt tatttccaca aagaagcttt tgaaaaacaa
     1261 ttttttaagt tacgcacgaa tttttatttt tctatcgatg atctcaaaga ggaacagcct
     1321 tttttcgcgc aaccaaaaga cttattctct tttatgatcg gatacgacgc cagtttgaaa
     1381 gaaagtatcg aacaaatcaa aacagctctt tactatccag atggcggatt accacttttg
     1441 attacaggcg aaagtggcac aggtaaaagt tatttagtta atttggtcta tcagtattgt
     1501 ctgcttcatg atctgctaga agattctgca ccattcatca cttttaattg tgcacaatat
     1561 gcagataatc ctgaactttt gactagcaat ttgtttggac atgttaaggg agcgtacact
     1621 ggtgcagaag aaaataaaaa aggggctttt gaagcagcag atggtgggat cttgttttta
     1681 gacgaggtac atcgcttaac accagaaggt caggaaaaac tgttcaccta tcttgatcaa
     1741 ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac acgtctcttt
     1801 tttgctacga ctgaagagat tactagtcat ttcttaacaa ctttcattcg aagaatacct
     1861 atccgtatcg aactacctgc acttaatcag cgtagtcgta atgaacggct agaactgatt
     1921 tactcttttt ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt cagtggacca
     1981 gctttgtcac ttttaacaag tcagaaatta tccggaaaca taggagagct gaaaaatatc
     2041 gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg aacagcctga aatccatgtc
     2101 acgatccatc atttgcaaaa agaactactt gcgcagccta ttactaaaaa tacagcacag
     2161 caaggaatct atattacgca agaccagacc ttagagcaat taatggagaa gcaacagccc
     2221 gaacagcagc gaatcgttaa aagttttgag caaatcctaa ttgatttcaa gaaaaataac
     2281 tgtctgctat attcttgcga aggaaaatta aagcaagaag tgattcaatt atttgatttt
     2341 ttgctgtttg agaccagtag acaagaaaaa cacgagctgc tggtttattg gacacaatct
     2401 atccgagaga cctttcgaca aatggaaact gcctatcaaa tcaaattcga tggaaacagt
     2461 gtctatgcct tgagttacta tctctatcag cgaagcacta tcaaatggtt accggaagaa
     2521 catgatatta tccaactaat caaagaatta gaacaacaag tttctcagtc ttatccatct
     2581 agttatcact atgtccaacg aattctggaa ttaagtaaga tcaaattaga tattgaagac
     2641 acaagtatgg atcggatttt actgacgatc tatctaaaaa aagcgaaatg gtcaaaagaa
     2701 aaacgactgc caaaagcgat gattgctgct cacggttatg caactgctag cagtatggca
     2761 aatgtagtaa atcgtttatt acaagaggat ctatttgaat catttgatat gccactagat
     2821 gtcacaccac agcagattgc ttcagaaatc cttgattatt gcgaaaacag cgatgtttca
     2881 aatggattga tcatattagt agacatggga tctctgaaag aaatccatca gttttttaaa
     2941 aaacagttat ccgtaccgct tttgattttg aataatgtga cgacaccttt agcaatcaca
     3001 gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga gactgttcga
     3061 cagatccagc cagaatggcg actgctctat ccagaggaaa acaaacccaa agcactcatt
     3121 actacttgct tcactggaat cggtacggcg gtccacttaa gtgaactgct agaaaaaagc
     3181 ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt aaaagataag
     3241 aaaaacagtg acccattatt ttccatatac gaagtcgtag gaatgattgg caccacagat
     3301 cctgatatca ccaatatcga ctatctttca ttagaagacc tcatctcggg ggaaaagatg
     3361 tctgtactag ctgaatggtt ggacactact atgaatgctt cagagaagga aatattcaat
     3421 cagcgcatca ttcgtaattt ttcattggaa aaagtactgg attccgtgac tattctagat
     3481 acagagaagg tgatgggaga aatcgatcat tttatgagag aattagaggt gcagttgaat
     3541 cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg tctcattgaa
     3601 cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg gaagcagtgt
     3661 caaaaagaag ggttatctgc tattaaatct gcgtttagtg tcattgaaga gcattatagt
     3721 gtcatgatcc ccaattctga gattgcctat atctatgata ttttgagtga agatacagag
     3781 tttctctcga tagaagaaga attttgaaca taagcaagtt gttgccacta
//
LOCUS       DACDRK010000134         3785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDRK010000134 DACDRK010000000
VERSION     DACDRK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.137"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(704..997)
                     /locus_tag="IWJ77_002667"
     CDS             complement(704..997)
                     /locus_tag="IWJ77_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9454211.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1400..2578)
                     /locus_tag="IWJ77_002668"
     CDS             complement(1400..2578)
                     /locus_tag="IWJ77_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9454212.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2682..2996)
                     /locus_tag="IWJ77_002669"
     CDS             complement(2682..2996)
                     /locus_tag="IWJ77_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9454213.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            complement(3316..3711)
                     /locus_tag="IWJ77_002670"
                     /pseudo
     CDS             complement(3316..3711)
                     /locus_tag="IWJ77_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1212 a          722 c          554 g         1297 t
ORIGIN      
        1 tacaaagatt ttatttttaa ataaaaaata ctcttttttt acgattattg aaaaaaaaag
       61 ataaaacttg attaattgaa gtattgctta aatttggaag ctatgaggga agaataaaag
      121 agttaggacg ggattatcta ctatttttct ttgttttaaa tatttgttat ctttgaacat
      181 aaaagagaaa tgtgtcgact gcagtgttgt gtccctcatc acgaataaac ggtgttcaat
      241 tagctgatct tgtattcact taaaaaatgg taaagtacga gttgctattc cccattttca
      301 attttctaac gctcaggaca aaatactgtt ttcacaacct cattggaata aaagcatata
      361 tattgtttca agaacgaaat cttgttatct ttttaaaggg catctgtcca aaatctaatt
      421 ttaggaggta cgttttggta tttattcgta caaataaaag agaattatct ttattcatgt
      481 aacgcatcca attgattttt tcatgtacgc tcctggaacg attttactga cgattgctta
      541 tttaactgcg aatcgctcat tagcttttat catagctatc catattttga ataacgttct
      601 tggctttgta ttatagatac agatgacaaa aaaagttcaa gtatgcggtg tagaaccaat
      661 atcgcatact tgaacttttt tcaatgagta taaattaaaa cttctaattt tcaattccca
      721 tcatctgata tgcccgaccg tctgaacttc ttttgaaaaa gccaaatgtg attagataac
      781 gtctttgttc gacataatcg ccactagaaa atacgttcag cagtatcgaa ttaacctctg
      841 tttctgtata agtcactcca tggtcaaagc gtttcaagac ttctttcaat aggatgtatt
      901 tcttttttgt tgaacttggt acttgtttta cttttccatc attcatgaat gtgtctaaaa
      961 ctttcatctt ttcttgctcc gtataagtat aaaccatctt ctttctcctt ttgattctaa
     1021 gaatactcca tttatgttag aatattctgg attttttatt aaagcggtta ctcttaagaa
     1081 agatattatc atagtgctct tttttttact actacttata ggtagattta ttctaatcca
     1141 ttattctccc ctaccaataa ttaaagaaaa aactggatca gggtgttcaa attacatgct
     1201 aagaaaaaag aacccttgaa taccaagggt tctttctcag tctgataaca atgattaacg
     1261 acggatttct ttgatacttg tgaaaagtcg ttaaagtatt gatataaaag gatttttatt
     1321 acctctgact acatattgac taatttgtta aaaaatcgtt tttcaagaat cctgtaatac
     1381 cagtgctttc accgtttttt taactctcta aagaactaaa agcaatcaga ggttttacct
     1441 tttcatttga cgttaagtgg cgataaattt tcgaagttgt ttggtcagta tggcctaagc
     1501 gatctttcaa gacttcccag ctacagtgtt ccgctactaa gcttgccatt gtatgacgag
     1561 ctaaatgagt atgaaatggc ttattaattc ctgagcgatt cacgcaccgt ttgacgtgat
     1621 catttacata ctctcgacga taaggaacac cacgtttact agaatcaaga tacacaaaca
     1681 aatatgtttc ttctataata aacctgtaat gttgcctcat ctcacccaaa gccttaatca
     1741 actgttgtag cttttccata actagaggag ttactagaac tcgtctagca gaactaactg
     1801 tcttagtagt ttcaattcga aaatccttac tcttaccttt aactgccgtt tgcttataga
     1861 acagaatttc atttcttttg aaatctatat ctccaggctg tagagcaagc gcctcgctga
     1921 ctcggcagcc agtgtaaaat agaacatgga aaagtacaga catcggtaat tcctcatgaa
     1981 caatgccata attcaaaaaa gttctcaatt catcgacagt aagaaacttg tcctcaatat
     2041 tatttatggc ttcttttaaa tcagttaccg ttaactgata ttttggcatt ctcacagact
     2101 tcattggatt ttcacttatc aaattctttc tttgacaata tttaaaaatc atatttagaa
     2161 cttgaataag tgattgtaaa ctatttttgg catagccaac atttgttcca ttaacgccac
     2221 attcctttaa ggtaaaaagc aattcttgaa ggcactctgg tgtcaaatca gacaactttt
     2281 tatcaccaat ccatcgaaca ataattttta attgttcctc tcgcttatca taggttattt
     2341 ccttttgttc atttaacgaa cggtaatatt ggaaccattg ctcacctaca acactaaact
     2401 tgtcattgct tgcaacaatt tttttcttaa gcctttgttg tttctccatt tccagataag
     2461 cgagttgcgc ctcacgtttt gaattaaagc cacgtcttgt tgttcgatat gcttgaaagt
     2521 accatgtgat cctaccagta gaactgattt gcttcttgaa tcgatctgtt ttttccattt
     2581 attgattcct cctattttga aggaacccaa caagcagaaa ccctttggaa atctttctat
     2641 ctatgtcatc attgtaccgc atcttagaaa gaaaatgtaa cttataaaga tcgaagtagt
     2701 cgcaattttt ctcgatagtc attcgaatca ctctttctca tccattctaa aacatcatca
     2761 cgaaaatagt gtcctcgaat cccgtaccaa cagggaggaa actcaccaag ttttactaaa
     2821 cggttcagtg tactatcact tatctgaaat agtcgcttta aatcagtatt tgttaatacc
     2881 agaggtaaat gactgactgc aagcaatttt tccgtgtaat cttccagttt tttttgtaaa
     2941 attgaataga actctatttc cagagaatta acatttgttt taacttccaa ctccatcacc
     3001 cttctctctc aaaatcaaaa cgaccaccac ggtacccctt aaaattgtaa aaaaacactc
     3061 cagtactcca tcaaaacgga ctagaaaaga ggaattttta atatgaaaca aggaattgca
     3121 gacataaaaa taattaaaga aattcttgaa aaatctacgg ctaatgcaat agcattcgga
     3181 acaggaatca acttgagtac agtaaaaaaa cttaaaagtg gagagagagc tgaagaaaaa
     3241 ttaaatcttg ccgatgctat taaaatcaca gaatttggca tgaaaaatat gccaaccaaa
     3301 atcgaaatat ggaaatccta gctcttccaa agccatttgt cgttggtacc aaacactgcc
     3361 gagacttttc ccaataatat cgccaatttc acgatcatta agaccgatga agaaggacat
     3421 aaaaatgatt tcttgtcttt gactaggtaa tgctgcaatc gcatttgcca actcctcatt
     3481 ggaaataaag attttcatac caaggacaat aaaagtacca ctactattca aatttggttc
     3541 caaataatag aactctgtac ctaacccttt ttgttccaat atttccattg atatttgccg
     3601 ttggcgaaat ctagcgtact tcttttgaat atttaacgcc tcattgcaga ctactttctt
     3661 acaaaaactg tcaaacgtat gttgaatgtg ggtttcctct gagtcgccca taatattctc
     3721 cccctcaaaa aattcattca actcgtttta cttctatccc tttccattat tactacaatc
     3781 tagga
//
LOCUS       DACDRK010000135         3735 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDRK010000135 DACDRK010000000
VERSION     DACDRK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.138"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(269..1567)
                     /gene="eno"
                     /locus_tag="IWJ77_002671"
     CDS             complement(269..1567)
                     /gene="eno"
                     /locus_tag="IWJ77_002671"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9454214.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1683..2438)
                     /locus_tag="IWJ77_002672"
     CDS             complement(1683..2438)
                     /locus_tag="IWJ77_002672"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9454215.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2542..3732)
                     /locus_tag="IWJ77_002673"
     CDS             complement(2542..3732)
                     /locus_tag="IWJ77_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9454216.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
BASE COUNT          944 a          782 c          700 g         1309 t
ORIGIN      
        1 ttatttatac gagaggactt gcccatagat tcctattcta ctgttctatc cttaaaagct
       61 cttatatgat gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca
      121 ttctttgtaa acatcatata cagaagctag ttgcaaaaaa actctctacc tgctgatatt
      181 tcagcaagta gagagttcga ttacttaact aagtgatcat actcgactat ctttcaatag
      241 tcgatcatgt atcttcatta gaaaagaatt atttgttttt taagttgtag aaagatttca
      301 aacctttgta ttctgcaact tcaccaagtt ggtcttcgat acgtagtaat tggttgtatt
      361 tagcaatacg gtcagtacgt gatagagaac cagttttgat ttggccagcg tttgttgcaa
      421 cagcgatatc agagattgtt gaatcttctg tttcaccaga acggtgagat acaactgctg
      481 tgtagccagc ttctttagcc atttcgatag cttcgaaagt ttctgttaat gtaccgattt
      541 ggttcacttt gataaggatt gagttagcaa ttcctttttc gataccttca gataattttt
      601 gagtgtttgt tacgaacaag tcgtcaccaa ctaattgaac tttgtcgcct aatacgtcag
      661 ttaatttctt gaatccgtcc cagtcgtttt cgtctaatcc atcttcgatt gagatgattg
      721 gatatttaga aactaattct tcgtagaatt tgatcatttc gtcagttgtt ttttcgcctt
      781 cgcctgagtc agctagtacg taaacacctt tttctttgtc gtagaattct gaagaagcag
      841 catccatagc aagaacaacg tctttaccag gtacatagcc agctttttcg attgcttcga
      901 tgattacttc aaaaccttct tcgtttgaac caaggtttgg agcaaatcca ccttcgtcac
      961 caacagaagt tgctaatccg cgagctttca agattgaagc taatgcgtgg aatacttcag
     1021 cacccatacg tagagcttct ttgaatgttg gagcgccaac aggcatgatc atgaattctt
     1081 ggaagtcgat tgagttatca gcatgtgatc cgccgttgat gatgttcatc attggtgttg
     1141 gcaatacttt tgtgttgaat ccgcctaggt agtggtataa aggtacttct aggtagtcag
     1201 cagctgcacg tgcaacagcg atagaaacac ctaaaatagc gttcgcacct aatttacctt
     1261 tgttaggagt tccgtctaaa gcgatcatag ctttgtcgat agccatttgg tcacgaacat
     1321 cgtagccaat gattgcttca gcaatgatgt tgtttacgtt gtcgacagct ttagttacac
     1381 ctttaccgcc ataacgagct ttgtctccgt cacgtaattc aactgcttcg tattcaccag
     1441 ttgaagcacc tgaaggaacc attccgcggc caaaagctcc gctttctgtg tatacttcta
     1501 cttcgattgt tgggttacca cgtgagtcta agatctcgcg agcgtaaaca tcagtaataa
     1561 ttgacatgtt ttgtctctcc tttgagtttg ttttttccta agggatttcc ctcatatacg
     1621 attttagtta attttgctca ttagtgcaaa catttcttgc atttttcgca aattttcaac
     1681 aattatttta cagcgtctaa caatgataag aatgaatctg cttgcaagct tgcgccaccg
     1741 actaaagcac cgtcaacatt ttcttttgcc atgtattcag caatattttc tggttttaca
     1801 gaaccgccgt attggatacg aactgcttct gatacttctt ttccataaag tttttcaact
     1861 gtttgacgta caactccaca aatttcgtca gcgatttgtg cgtcagctga tttaccagta
     1921 ccgattgccc agattggttc ataagcaata accatgtttg acacctgttc agctgaaaga
     1981 tcagctaaac cagctgtaat ttgtccttcg atccattcag cagttttgcc tgcttcatag
     2041 gtttctaaag attcgccgca gcataggatt ggtgtcatac catttgcaaa gatagctttc
     2101 gcttttttat tgatgtcttc gtctgtttca tggaaatatt cacgacgttc tgaatgtccg
     2161 atgatcacat aatgaacacc tagatcagca agagctgctg gtgaagtttc accagtgaac
     2221 gcacctgagt tttcccagta gcagttttgt gctgagattt ttagttctgt accttcagct
     2281 gatgtcatta attcttgtaa gaataatgca ggagatccga tgactgaatc aactttgtca
     2341 tttgaaggga ttttgttttt tactgcttca gcaaattctt ttgcttctga tgcagttttg
     2401 ttcattttcc agttacctgc gataataggt ttacgcattg aggaacacac tccttaattt
     2461 attgaaaaat ggctagggag tcatgaaaac tcccttcacc ttttgttcga ttactcgtca
     2521 gaattgagca aacttggtcg tttatttatc gttgattgct gctagtcctg gtaattcttt
     2581 accttcaagc aattccaagc ttgcgccgcc acctgttgag atgtgtgtga atttgtcagc
     2641 aaatcctaat tgttctgctg ctgcggctga gtctccgcca ccgatgattg ttgttgcgcc
     2701 ttctaagtta gcgattgctt cacaaacacc gattgtacct ttagcaaagt tactcatttc
     2761 aaatacaccc ataggtccgt tccaaacaac tgtttttgca ccttgtaatg tatcagcaaa
     2821 taattttact gatgctgggc cgatgtctaa agccatcaaa ccatcaggaa tgtctccttc
     2881 gattacttgt gtttcaacat catttgaaaa ttctggtgca cagacattgt cgattggcaa
     2941 gacaagtttg tcgcctgctt tttcgattaa ttcttttgct agttccactt tatcagcttc
     3001 tactagagag ttaccgatct tgattccttt tgcttcgtag aatgtgtaag tcattccgcc
     3061 accgatcaag attttatctg ctttaggaat caagttttcg attacgccga ttttatcaga
     3121 aacttttgcg ccaccaagga ttgcaatcat tggtcgtttt ggttcttcta ctgcttcacc
     3181 gatgaacttg atttcttttt ccatcaagaa tccagcaact gttgggatac cagttgaagc
     3241 gatacctacg ttagaagcat gtgcacggtg agctgtaccg aaagcatcgt taacaaatac
     3301 atcgcctaat gaagcccagt atttacctag ttctgtatca tttttgcttt cttttttacc
     3361 gtcaatatct tcaaatcgtg tattttcgaa tacgacaacg tcgccgtcat tcatgttgcg
     3421 gattgcttct tctaattgtt ctccgcgagt ttcaggtacg aatgttactt ctttacctaa
     3481 taattcgcct agacgttttg caactggagc taaagatttt ccttctttat cttcttctgt
     3541 ttttacacgt ccaaggtgag agaaaaggat tgcttttccg ccttgttcta gaacgtagtt
     3601 gattgttggc aatgctgctt tgatacgagt gtcgtcagtg atcacgccgt ctttcaaagg
     3661 aacgttgaag tcaacacgga caagaacttt tttgtctttt aaatcgatat ctttcactgt
     3721 tttcttagcc atttt
//
LOCUS       DACDRK010000136         3713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDRK010000136 DACDRK010000000
VERSION     DACDRK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.139"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWJ77_002674"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWJ77_002674"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9454217.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IWJ77_002675"
     CDS             complement(2261..2512)
                     /locus_tag="IWJ77_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9454218.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IWJ77_002676"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IWJ77_002676"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9454219.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IWJ77_002677"
     CDS             3462..3680
                     /locus_tag="IWJ77_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454220.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DACDRK010000137         3654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDRK010000137 DACDRK010000000
VERSION     DACDRK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.140"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..1848
                     /locus_tag="IWJ77_002678"
     CDS             133..1848
                     /locus_tag="IWJ77_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9454221.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1851..3614
                     /locus_tag="IWJ77_002679"
     CDS             1851..3614
                     /locus_tag="IWJ77_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9454222.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         1087 a          721 c          732 g         1114 t
ORIGIN      
        1 tctaaagctt cttcataata aaactttcat ttatgaaaag gcttgttctc ttttcattct
       61 aaatttccta agctacaatg tggtagtact tgttttttca attgtggaca aataaataat
      121 aggaggaatc atatgattca gttattacgt tatgcaaaag actatcgtaa gcaaatcatt
      181 ttgggcccgt tcttcaaatt tcttgaagct gtcttcgaac ttgtcctgcc tttaatgatg
      241 gcttccttga tcgataatgg actaaaaatg aatgaccgag gaaaaatcat tgaaatgggc
      301 ttatggatgg tcgctatgtc tgtgattggt ctgatttgtg ctatcatttg ccaatactat
      361 gcttcagtag cttcacaagg atttggtaca gagttacgta atcagctaat caaaaaaatc
      421 aatactttct ctcacaacga gttgaatcat tttggaacag ataccttgat cacccgaatg
      481 acaaacgaca tcaatcagct tcagctagct ttagcaatgg tcattcgttt attgatcaga
      541 gcaccgtttt taagtatcgg gtcagtggtc atggcttttg tgatcgactg ggaagtaggt
      601 ctgtttttct tagcattatt gcctattttc tcaatcattt tattttttat catcaaaaaa
      661 accgttcctc tctaccaaaa agtgcaagaa aaactagatc agctcaatga acacgttagt
      721 cagaatctta gcggtgttcg tgtcatccgc gcatttgcgc agacaaaaaa agaaacagag
      781 aagttcgata aaagcacaaa tgatctagcg aacaattatt tacgtgtttc caatatctct
      841 gccctattat cacctgctac aaccttgatc atgaatgtcg ggatcatctg tttgctgaca
      901 gttggcggga tcaaagtaaa tattggtagc ttgcaacaag gacaagttct tgctttgatc
      961 aattatatga atcaaatgct tttggcactg atcgttgttt caaacctcgt cgtgatcttc
     1021 acacgtgccg aagcttcagg aaatcgggtg aaagaggtgc tagacacaga aaattcgatt
     1081 cttgaagctg aggtcagtac tactcccgat ttttcttctg aagcaattat cgcttttgac
     1141 catgttgact tccgttatac tcctgattct ggtctttctc tccaaaatat tacatttcag
     1201 ctgaagcgaa atacagtcct tggaatcatt ggtccaaccg gaagcgggaa aacgactttg
     1261 acacaattga ttccgcgttt ctatgatgtc agttccggta cagttataat ggacggaatg
     1321 gatgttcgtt cttggcagct ggatcatctc cgtcagcaaa ttgctcttgt tccccaaact
     1381 tctgtactat ttaccgggac tattcgtgaa aaccttcaat ggggtaagaa agacgccaca
     1441 gaaaaagaat gttgggaagc tctgaaaatc gctcaggcag aagaatttgt ccgtcagctt
     1501 ccaaatggtt tagatacaaa agtcatggaa aatgggaaaa atttctctgg cggacaaaaa
     1561 cagcgactga cgattgcaag agctttgatt gccaagccag cattactgat tttagatgac
     1621 tctttaagtg cgttggatta tcaaacagac ttggatttgc gtaaggcgct acagacttat
     1681 cttggcagta cagtgattat tatctcccaa cgtgtccgct ctattcaaga agcacatcat
     1741 atcttagtga tggatcaagg taagattgct gctcagggta cccatgaaga attacttgct
     1801 caatcagcag aatatcagga aatcgtcgca tcacaggagg aagaatagtt atgaaagtaa
     1861 aaaaattatc cttgcagacg ctccgccgat tcattcctta ccttactgcc tatgcaaaag
     1921 acatttggct agctgtgata ctaggaatca tcaatgggat tgcaacagtt gtgatgacgt
     1981 tacaaattgg tcaaagtatc gatcaaatga ttggagaagg acaggtccgc ttcaatcgac
     2041 tgttccattt acttattcta ttcggtggta tcgttttaat caacgtagtc agtcaatggc
     2101 tcatccaagt gttaagcaat cggttagctt acttatccgt tgcaaaatta agaaaagatg
     2161 catttgctca tctgaatcga ttgccacttc gttattatga tcaaaactcc cacggaaata
     2221 tcgtcagtcg atttacaaat gatatggaca atatttccat tgcaatttct gcggccttca
     2281 atcaattatt ttctggagtg gcaatcatca ttctttcgtt tatttttatg atgcgtctaa
     2341 gtccacttct gacattggtc gttctttgtt ctacaccaat tattttcctt gtgagttgga
     2401 tcgttgctcg tgcttcacaa acaaatttcg atagacagca aaggattatt ggggatatct
     2461 cgggctttgt gaccgaacga atcggtaacc agaaaatcgt gaaagctttt cagcaagaag
     2521 aagctagtca acagcaattt gaactgctaa atgctgatct atatgaaaaa ggacaacggg
     2581 cacaattttc ttcttccttg acaaacccgt cttctcgttt tgtagatcac cttgcttatt
     2641 tatcgattgg tttagttggc ggcctattgg cattgaagag tggttcgtca atcactgttg
     2701 gtgtgatttc cagctttacg atctactcaa gtcagttttc taaaccattt atcgaacttt
     2761 ctggaatcac gacacaactg caaaccgctt tttctggtct agaacgagca tttgatttga
     2821 tcgatcagcc agaagaaaag cctgatttgc cagatgccta tgccttggaa cccaaaaata
     2881 tcaaaggaaa agtctctttt gatcatgttt ctttttctta tgaccccaag cagcgtttga
     2941 tcgaagattt caattttact gcttatccag gtcaaaccat tgctatagta ggaaaaacag
     3001 gggccggaaa atccacactt gttaatttgc tgatgcgttt ttatgacaca acaaaaggaa
     3061 caattacagt cgatgaccat gacatccgct ctatccaacg tgatacatta cgtaaaagtt
     3121 ttggaatggt gttacaagat acttggctat ttgacagtac attgcgagaa aatcttcgtt
     3181 ttgggaatcc ggaagcaagt gacgcagaaa tcaatgatgc cctaaaagcc tcttatatgt
     3241 atgaatttgt agaacgtttg ccggaaaaat tagatactaa gattggcggt caagggttaa
     3301 agatatcaga tggacaacgc caactgttga cgattgctcg aacgatgatc agtaatcccc
     3361 ctatgttgat cttagatgaa gctaccagct cagtcgatac attgacagag aaaaaaatcc
     3421 aacaagcctt cttagcaatg atggaaggaa aaaccagttt tgtgattgcc catcgcctgt
     3481 ccacaatcaa aaatgcggac cagatattag tgatggatca gggagctatt gtcgaaatgg
     3541 gcacccatga tgagctgctt caaaaagatg gttattaccg acggctctat caagcacaat
     3601 tcacagcaca ataaaaacaa gcagtcttct gggatgggaa gactgcttgt tttt
//
LOCUS       DACDRK010000138         3602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDRK010000138 DACDRK010000000
VERSION     DACDRK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.141"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            120..1094
                     /locus_tag="IWJ77_002680"
     CDS             120..1094
                     /locus_tag="IWJ77_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9454223.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1076..1537
                     /locus_tag="IWJ77_002681"
     CDS             1076..1537
                     /locus_tag="IWJ77_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9454224.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1587..2051
                     /locus_tag="IWJ77_002682"
     CDS             1587..2051
                     /locus_tag="IWJ77_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9454225.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2065..2814
                     /locus_tag="IWJ77_002683"
     CDS             2065..2814
                     /locus_tag="IWJ77_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9454226.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2814..>3602
                     /locus_tag="IWJ77_002684"
     CDS             2814..>3602
                     /locus_tag="IWJ77_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9454227.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGC"
BASE COUNT         1218 a          481 c          693 g         1210 t
ORIGIN      
        1 aaattgttat aaaaaaattt ttttgaaaaa tagtattgca aaataaagca atttcataga
       61 taataggtat tgtaaagtta tatgacttta taatacttat tagagtgagg gataaagaaa
      121 tgagacaact acacaaagaa cagattgaaa cagcaatggc agcgttaaag gaaaagacaa
      181 ttgatcatgt atattttaca gcatgtgggg gttcacaagc cgttttaatg gctggtcaat
      241 atctatttga taaagaatta actgtacctt gtcatgttta tactgctaac gaatttaact
      301 atgctatacc aaaaggatta tctgaaaatt ctttagttat cacttgttcc cattctggca
      361 caacaccaga aactgtagaa gcaacaaaaa aagcaagaga atttggagca atgactattt
      421 gtttatctaa tgaagaagga tctcctctat gggaagcagc tgaatatcct gttcactatg
      481 attggggtaa agatgttgat gctagtgatt taaataaagg aattctttat ggtttattat
      541 ttaatatttt acttgtttta gatggctcta aaaaatggga aacttgcttg aatgaatttg
      601 ataagttaaa tgatcttgtt attgctgaaa agagcaaatt tgaaataact gctaaagaat
      661 ggggtaaaaa ccaaaaacga gataaaatta tttacacaat tggtagcggt atcaattacg
      721 gagaaattta ctccacagca gcttgctggt tcatggaaat gcaatggatt aattctaatg
      781 ctattcattc tggggaattt ttccatggtc cttttgaaat cactgattat gatgtaccat
      841 tcatcttagt gaaaagtatt ggagatacaa gatttttaga tcaacgggta gaagacttca
      901 ctaaaaaatt cagtaataaa ttaatggttt tagaccaagc tgattttgac ttagaatcta
      961 tagatagtgt agcgaaagaa tatattgcag ctgtacttag tggcgttgtt attcgccaaa
     1021 tggtagaagc gattgctttt gaaagaggcc attccttaga tgttagacgc tatatgtggc
     1081 aaatgaaata ttaattaata ggaggttgca aaagatggca aatcaattat ttcacattgt
     1141 gattacaaca cattctaccc tttgtgaggg ttatttaaag gcaacaaaat taattttaat
     1201 gaatgacgcc acaggtatta gtgctgttcc atttaaagaa aatactagtc cagatgattt
     1261 tgaggagagt atgaaagaaa taattagtca gcatcatttg caacctctaa ttattttaac
     1321 ggatttaatt ggaggaacac ctaataatgt ttctattaaa cagttgaaca acaatagaat
     1381 tcaaattatt gctgggatta atttacctct tttactggag ttattaatgg cccagtcagc
     1441 tggtaaatct atagaagaaa tagattttaa ggaattactg aataactctc aaaaaagtat
     1501 ggttcatatt aattcgcttt taaaagatgt cagttaaggt ggcaatttct attctataaa
     1561 ttttaaaaat tgaaggagga aatagaatga ttaaattagt gcgtgtagac cacagattac
     1621 ttcatggaca agtaatattt tcttggacta aacagtatga tattaaccat attattgtgg
     1681 ctgataacaa agttccagga gatccaatgt ctgtgatggc tttgacaatt gctaaacctg
     1741 ctgattgcaa attagacatt gttaaaatta gtgatgttaa agggattatg gaaacatccc
     1801 caaatgataa ttacatgatt ttaattaaag gacctaagga agcttttgaa ttagttcaaa
     1861 atttaccaga aattaaagaa attaattttg gcggtgtgtc taagaaaagc gagtccaaac
     1921 aatatggaaa ggcagtcttt ttaactcctg aagaattaga agccacaaaa aaaattcttg
     1981 atcaagaagt gaaaatattt gtgcaacaag tcccatcttc aagtattgaa aatgtagatt
     2041 ttagtaagta aggggggaaa ataaatgtta ttaaaagcca ttttattagg tttagttggt
     2101 gtggtagctg ttattgactc caggttaatt ggtcgacaaa atattggtag accactaatt
     2161 ttgagtacac ttgccggact tgtgctaggt gacttgcata cagggattgt tcttggggca
     2221 tctttagaat taatttctat gggattcgtc tcaattgggg cggctggacc tcctaatatg
     2281 caacttggta gtattattgc cactgctttt gcaattttaa ctaatagtag tacagaaaca
     2341 gccttaacaa tagctatccc agtggctgta gctggtgaat ttatgagtat tatcatgcgc
     2401 atgataattg ctcaatttgg acatgctgca gataaagcca ttgaaaataa taaatttaga
     2461 aaagctcaaa ttattcatat ttattggtca tttattttta atgcttttgt ttactttatt
     2521 ccaatctttt taacagtata ttttggtgct gacgttgtgg cagatttagt agataaaatt
     2581 ccagaaatga ttacagacgc cttgactgtc tctggaaata tgctttccgc attagggttt
     2641 gcaatgctat taagcacaat gttgagtaaa aaattatatc cttactttat ttttggattc
     2701 tttattgttg cctattcagg tctttcacta attggcgtga caatttttgc agctctgatt
     2761 gcctttgtaa tggatcaagt taaatatgga aaaagagaag aggcgcatgg ttaatggaaa
     2821 atagtacaga aaaaaaacgt ttaatcacaa aaaaagatct taataagatg tgtcttcgtt
     2881 catggacaat catgtgttcg tggaattatg aaagacaaat gcatatggga tttatgtatg
     2941 ggatggcgcc aattttaaat aaagtttata aaaatgatga agagaaaaaa agagaagctt
     3001 atactcgtca tatggcattt tttaactgta cgcctcaaat gacttcattt attatgggac
     3061 tttgtgcttc tatggaagaa caaaatgctc aagcagaaga aggaaacttt aatactgaat
     3121 ctatctccat gattaaaacc agtttaatgg gtccttttgc tggaattggt gattcatttt
     3181 tccaaggtac tttaagaatt attacatttg ggattggatt gtcttttgct aagcaaggta
     3241 gtattttagg accgatttta gctgttggcc tttttgctat accttctatt ttagctgttt
     3301 tttacggcgg taaattagga tatgagtctg gcaataaata ccttactaaa ttgtatcaag
     3361 aaggtcttat ggatcgagta atgcaatttg cttctatcat tggtttggct gtagtaggtg
     3421 gaatggtggc tagcatggtg ccaattgtca cccctttaaa attcactaca gggggaacag
     3481 aacttgtttt acaagactta cttgattcca ttattcctgg attattacca cttgtatgta
     3541 ctttgggaat atataaattg attaagaaaa atgttagtac aaatatgctc ttattaggtt
     3601 gt
//
LOCUS       DACDRK010000139         3128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDRK010000139 DACDRK010000000
VERSION     DACDRK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.142"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IWJ77_002685"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IWJ77_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IWJ77_002686"
     CDS             1360..1992
                     /locus_tag="IWJ77_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9454228.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IWJ77_002687"
     CDS             2197..2424
                     /locus_tag="IWJ77_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9454229.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IWJ77_002688"
     CDS             2493..2759
                     /locus_tag="IWJ77_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9454230.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IWJ77_002689"
     CDS             2749..3018
                     /locus_tag="IWJ77_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9454231.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DACDRK010000140         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDRK010000140 DACDRK010000000
VERSION     DACDRK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.143"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IWJ77_002690"
     CDS             177..359
                     /locus_tag="IWJ77_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP9454232.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IWJ77_002691"
     CDS             462..746
                     /locus_tag="IWJ77_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454233.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IWJ77_002692"
     CDS             762..998
                     /locus_tag="IWJ77_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454234.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IWJ77_002693"
     CDS             1020..1274
                     /locus_tag="IWJ77_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454235.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IWJ77_002694"
     CDS             1356..1541
                     /locus_tag="IWJ77_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9454236.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IWJ77_002695"
     CDS             1608..2003
                     /locus_tag="IWJ77_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9454237.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IWJ77_002696"
     CDS             1993..2370
                     /locus_tag="IWJ77_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454238.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IWJ77_002697"
     CDS             2371..2766
                     /locus_tag="IWJ77_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454239.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DACDRK010000141         3010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDRK010000141 DACDRK010000000
VERSION     DACDRK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.144"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..288
                     /locus_tag="IWJ77_002698"
     CDS             <1..288
                     /locus_tag="IWJ77_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9454240.1"
                     /translation="KTLFRLLMQYRSTGYYVVRIEEFRSLMDIPEYYQMGNIDQKVLN
                     PALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKISLRNWLEE"
     gene            complement(425..841)
                     /locus_tag="IWJ77_002699"
     CDS             complement(425..841)
                     /locus_tag="IWJ77_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454241.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(844..1128)
                     /locus_tag="IWJ77_002700"
     CDS             complement(844..1128)
                     /locus_tag="IWJ77_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454242.1"
                     /translation="MDILVRNIDPKFVATIDKLCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1828..1923)
                     /locus_tag="IWJ77_002701"
     CDS             complement(1828..1923)
                     /locus_tag="IWJ77_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9454243.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2576..>3010
                     /locus_tag="IWJ77_002702"
     CDS             2576..>3010
                     /locus_tag="IWJ77_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9454244.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAK"
BASE COUNT          961 a          477 c          565 g         1007 t
ORIGIN      
        1 aaaactctat tccgactgct tatgcagtac cgttctactg gttattatgt agttcgtatt
       61 gaagagttta ggagcttaat ggatatccca gaatactatc aaatgggtaa cattgatcaa
      121 aaagtattaa atcctgcatt acgagaacta aaaaaatact tcaatgatct taaagtcaca
      181 aaaataaaag caaaaaaggg caataaaatc gccaaattag aatttacttt ttcaagccta
      241 acgtcagatt tgccaaagat ttctctgcgt aactggttag aagaataaat aaaaaagaac
      301 tcgcccccct tacattaagc aagtagacaa gtcccttaca accgactaag attgctagta
      361 cattctagca tattgtatgg ggcttctaaa taaaaaacag ataggaataa ctcctatctg
      421 ttttttatcg ttttagaaaa tttgttgggt cgaaatttct agaagtgact gtttgactgg
      481 tttctttttg atgattcttt ggtcgatttc gatttgatgg atttagttta gcttcgattt
      541 tttctttcac ttcttgatat actttagaat ttgtgccaat aagaacgcct gtatctttta
      601 gatataaatc agcaaattct gcgatgattt cagtcatgat ggaaatttct tttcccatag
      661 catttagttt tgcctgattg ctggtagtgg tcgtgttggt atcgggttca taactcatac
      721 ataaaaagcg aatggcttcc gagtagttat tgatatctgg atttttttcg atgatggaat
      781 ctaaaacgtc aacatgacta gggtacaaag taacatgttt ttggacagaa gtttttctca
      841 tggttatatc attcctccga taagcatttc aatgatttga ttatttgtgt agacatactc
      901 gcttaatatt tgagtatttt ctgtttggat agcaataaat ttatcgagca ataaatcgaa
      961 ttggtctttt ttgtaagtcg taagttgacg gtcaaaatca ctttcgatta aaacttttag
     1021 atatttattt cgactccatt tttctcctgt tttattcgtg agttcttggc aaagtttgtc
     1081 tattgttgca acaaactttg gatcaatatt ccgaactaaa atgtccataa aaaatctcct
     1141 tttgcaatta tgtataattt atgtatacca tacaaatact atatttgtat agctgttttt
     1201 tgccaaaatg ctatacataa gctacccacg atggcgaaaa agtgatatca aatttttttc
     1261 tgagaaacgt gataggcaca gcctatctac ccactttttt tagaaagtgg gtacacgttt
     1321 ccttatgctc tttaaaattc gggggggagt gctgcacgtg taatcgataa ccaaatttaa
     1381 cagacgaaaa attgttggaa tataaaaata tgacacccga aagaaaacga tcgttggaaa
     1441 aattaatgca actagtgaac gaagacggcg gaatttcaga cgcagctttt gacgcatgga
     1501 gcgaatttca tggagaatat aattaagtgg gtttaaatgg ctctaaaatc gcttctaaga
     1561 gttttttagc ttggaccaat gaaatacacc aaaaactaaa taaaacgtct taaatcgaaa
     1621 aatacgagct ctcagagcga ctttatttgt aaatgttgca tataagaaat aattacggat
     1681 tttagacttt tgtaggatat ttttgaaata aatatttatt tctgttataa tttgattgca
     1741 aataagtgaa cacgaaacac acgaaatccc cagagaggtc ggattctctg gggctttttt
     1801 tgtgggctga gtcgccttaa ttaagtttta cttcttgttt cggttattca accagtaact
     1861 gaacaaagcg cttaaaagcc caacaagaag ggagcaaata aagttagtga acacgaaaca
     1921 cacctccttc cagtcgcaag actgtcggat aggcgacaat caattataca ataaaaaagc
     1981 aaaaataagc ctaagttttt tattgccttg gggttgttag ggcttgccct gaccgcctgt
     2041 aaggcgcttt agaacgaagg acatagtttt tatcgaatct tgtcttagaa cggcttagac
     2101 gagctctcag gacgccacaa aggagtccct aatgaattat ttttggcgaa ggagcctaaa
     2161 ggcgactgct gcaagtaaaa tgtgagagcg tagcgatcac tccttcttac agcgaccata
     2221 gggagctaga ttggcagttt aatatcatgc gcgaacctgt ttattttaaa aagtgggtga
     2281 ttttttggcg ggaaattttg ctcgcgcgcg cgcgtttatt ttatttattt tttaaagatt
     2341 ttttagaacc ctttttaggg ggcagttgag ccttactctc ccaacggata cagaagggta
     2401 ctaacaactt tttatctgag tactaacaac tttttatctg agtactaaca actttttatc
     2461 tgagtactaa caacttttta tctgagtact aatatccttt tgtataataa caaccaacct
     2521 atttacgagt tggttgttaa aggttattat ttatttgtaa ggggggaaga aaggaatgga
     2581 tgaaaaaacg gaattaacag tcaaatatca aaatgaattg aatttagtac cgctgaaaaa
     2641 tttcaatgct aagcagatgg atttattttt tgcattatgc gctcgtatga aagatcgtgg
     2701 tttgaaaaaa gttagatttt ctttcgagga attaaaagag ttaagtgatt ataagatgac
     2761 tgctacaaaa gcctttgtcg gtgatttaga aaagctctat aaagatatgc ttaatcttac
     2821 ttaccgtact gaagatgagg attctattcg ctactttgtt ttgtttactg gttttgaaat
     2881 caataaaaca aaaaagtttg ttgaagtcag tgttaatccg gacctagaat atattattaa
     2941 tggtttaaca gctgaattta gtagatttga attatctgct ttcacgtcgt tgcgttctac
     3001 gtatgcaaaa
//
LOCUS       DACDRK010000142         2905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDRK010000142 DACDRK010000000
VERSION     DACDRK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.145"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            529..>2905
                     /gene="esp"
                     /locus_tag="IWJ77_002703"
     CDS             529..>2905
                     /gene="esp"
                     /locus_tag="IWJ77_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9454245.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKD"
BASE COUNT         1052 a          444 c          575 g          834 t
ORIGIN      
        1 gtcatttttt gtttcgctaa aaacgagtga aaaaatgttg ataactccaa tcattcgttt
       61 aagattctag gaatcattca gaaatgctgt gaaaccgata ctttataatt ataaagaagg
      121 tgatttcaaa ttccgaagaa ggaatttgaa gtcacctttt tacttatgtc acagtctcat
      181 tcagtaatgc gggataaggc tccttattcc gttatttgaa tactctgaag tttaatctaa
      241 gaaagggtaa tattttgaaa acatgcttca tcgcatagaa atgaacttaa tttcagaaag
      301 aatacataca tatcttttta tatgttttca gctgctgaat ggttaaaaag cacaaatatc
      361 cgaaaaaaac atgaagaaat gtactttttt tgttgtaatg ttcatgaaat tctctaagta
      421 aattagttaa cataaggtta gtaagattcc tattctgtga ataatacgta tcagatttat
      481 cttttattct tactaggaaa gtaattatta agggagttga tttgaataat ggttagcaag
      541 aataataaga gagtatttct agaaaaaaca aagaaacgag tattgaagta cagtatcaaa
      601 aaattaagtg ttggggttgc atctgttttg attggtgttg gtcttgtctt gggaacaact
      661 gaactagtta aagcacaaga tgaaatttct ccaagcagcc cacttgaaac agcgataagc
      721 tccgtacaag taggtgacaa agtagcttcg gggaatactt ttcaggagaa tcctggttat
      781 acgaaaaatt ataacttttc ggatttacaa tttagtcctc aagaactaac tggagacaca
      841 ctaaaaggca atacaattgg atttgaagtt tacggaaaac ataatattgc agcatcaact
      901 aaaaactggg aaatccgtct tcaattagat gaacgattag ctaaatatgt agaaaaaatt
      961 caggtcgatc caaagaaagg aattggatct tctagacgga cctttgtaag aattaatgat
     1021 tcgctgggta gacctacaaa tatttggaag gtcaattata ttcgtgcaag tgatggatta
     1081 tttgctgggg cagaaacaac cgatacgcaa accgctccta atggggtaat tacgtttgaa
     1141 aagagcctag atgaaatttt taaagagata ggcatagaca atctcaagac cgatcgttta
     1201 atgtatcgaa tctacttggt aagtcatcaa gacgatgata aaattgtacc gggtattgat
     1261 agtacgggct actttttaac agattcagat gatttctata atagcttaga tgtatctgaa
     1321 aataatcctg accagtttaa acatggatca gtaagtgcaa aatatgagaa acctaatact
     1381 caaacaaaag atggttcagg atccacaggt gctaatggag ctataatctt agatcataag
     1441 ttaacgaaaa attataattt ctcatattca tcttccgcaa agggcacgcc ttggtatgct
     1501 aattacaaaa ttgatgaacg attagttcca tatgtagcgg gtatacagat gcacatggtt
     1561 caggctgaca aagtaacata tgatgtttcg tttgaatccg gaacaaaagt agctgattta
     1621 gcaatcgagc gacgggaagg tcatgaaaat tatggcatag gttcaataac taataatgat
     1681 ttaactaaac ttatcgattt tgctaatgca agtccacgtc cagtcgttat tcgatatgtt
     1741 ttacaattga ctaagccatt agatgaaatc ttagaagata tgaaagcaac agcacaagtt
     1801 gaagaaaata aaccatttgg tgaagatttc atctttgatt cttggttgtc ggatacgaat
     1861 aaaaaattaa tccagaacac ttatggaaca ggttattatt atttgcaaga tattgatggt
     1921 gatggaaacc ctgacgataa agaagagagc ggagacacga atccatatat cgggaaacct
     1981 gaattagaag aagtatatga tgttgacaca acagttaagg ggaaagtatt catccacgag
     2041 ttagcgggaa caggtcacaa agcccaactt gttgataaag aaggtactgt attagcagaa
     2101 aaaactatcg ctccaaatga aaaagatggg gctccaattt cagatactgt agaatttgaa
     2161 tttacgggtg tagattcaag taaattaatc gcgaaagatg aattaaaaat ccaaatcgtt
     2221 tctccaggtt ttgataaacc agaagaaggt tcaaccgtta ttaaggaatc accaaaagcg
     2281 gttgataaac aaaccgtggt agttggattt aaaccagatg ctaaagaatc aattcggaat
     2341 aataaaaact tacctgaaga tgcagagtat tcatggaaaa cagagcctga tacttctaac
     2401 gttactgata gtacgaaagg tattgtaact gttaagatcg gaaatcgaac tttcgacgtg
     2461 gatgtagagt ttgctgtaaa agcttctcaa gctatggaaa atgatgcaac atacgtacct
     2521 ataacaacaa ctccagaaac gacagttcaa agtggaaaac ctacatttga taaaccagat
     2581 gttcctttag ctaaagatgc cttttcaatt tcagatgtct ataataagta cttcggtaat
     2641 gcaagtgttg atgcaaacac tggtattgtt acattcactc cagctaaagg tgtaggagaa
     2701 tcggagccga ttactggaac aattcctatt aaaattgttt accaagatgg ttctgtaggc
     2761 acgaccgatt tagcagtaac tgtaagtaaa gatatttatg aaaatccagg agaaaacatt
     2821 cctgcaggct accacaaagt aaccttcacc gcaggggaag gaacaagtat tgaaagtgga
     2881 acaacagtct ttgcagtgaa agacg
//
LOCUS       DACDRK010000143         2844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDRK010000143 DACDRK010000000
VERSION     DACDRK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.147"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..494
                     /locus_tag="IWJ77_002704"
     CDS             144..494
                     /locus_tag="IWJ77_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9454246.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="IWJ77_002705"
     CDS             908..1726
                     /locus_tag="IWJ77_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9454247.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="IWJ77_002706"
     CDS             complement(1799..2158)
                     /locus_tag="IWJ77_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9454248.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          969 a          399 c          505 g          971 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa
     2521 ggattactgg tttttctaaa atagtttagt ttaaatacat aagtatcctc cgacactgta
     2581 ttagttcata atcatctaaa tacaaaattt tcaaataaaa atatttcaag ctctatcgtt
     2641 gacgaatgta aacgatactt ataaagtaat agaaattgat tacatttgta aacttaaaat
     2701 ggttaattta tctcacataa aactaagaat aaaataatga ttaaaagtgg ttagttttta
     2761 tacgatttag cctatcggta aaaatgaatt agagagtgta aaatattttg tgtaaatgaa
     2821 aaaatccata caaaaaagga agtc
//
LOCUS       DACDRK010000144         2838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDRK010000144 DACDRK010000000
VERSION     DACDRK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.148"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..733
                     /locus_tag="IWJ77_002707"
     CDS             11..733
                     /locus_tag="IWJ77_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9454249.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="IWJ77_002708"
     CDS             791..2830
                     /locus_tag="IWJ77_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9454250.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIIAAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
BASE COUNT         1016 a          498 c          612 g          712 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tgcagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgc
//
LOCUS       DACDRK010000145         2826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDRK010000145 DACDRK010000000
VERSION     DACDRK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.149"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1273)
                     /locus_tag="IWJ77_002709"
     CDS             complement(<1..1273)
                     /locus_tag="IWJ77_002709"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAP9454251.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKS"
     gene            complement(1332..1793)
                     /locus_tag="IWJ77_002710"
     CDS             complement(1332..1793)
                     /locus_tag="IWJ77_002710"
                     /inference="COORDINATES: protein motif:HMM:NF012360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP9454252.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            complement(1804..2580)
                     /locus_tag="IWJ77_002711"
     CDS             complement(1804..2580)
                     /locus_tag="IWJ77_002711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9454253.1"
                     /translation="MAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGDFSFAGL
                     YLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMTLYYLFN
                     INRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSLNAFSDKYLI
                     GYGLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKSGCYKWL
                     FLMIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKKES"
BASE COUNT         1046 a          518 c          330 g          932 t
ORIGIN      
        1 aagatttcca aacctgcttt aaaattttaa atggattaaa cttaaaacca aaccataaaa
       61 taatccaaaa gactataatc tgctctatct taacgaaaga tctaccatat gctagttcta
      121 taaacccttt ccttgaatag attattagaa ctggaattaa tactgctaaa tgtaggatag
      181 tcataagaac atttgctttt ggttttccta aaccacgata gtactcagag gcatattgtc
      241 ccaagacaag agtaaaacag ctcattaatc catatatccc aacaaatggt acagcctctt
      301 tccattgact cccgagtaga atatttgtta ctagttccct atatacatat atacctatac
      361 ccagaggaat aataaaaatg gaaacaattt taataaaatc taaatatgtc ttatcatact
      421 ccgttttact attttgtaaa cgagataatg ttgaaaataa tacagttgtt gttgctgaag
      481 ttacaattgc aaaaatacca ttaaccgttg tcattgtagt tttgtatatt cccaaatagt
      541 atacgcttaa aatgcctcct acgataaaag tacctatata agatgacaac catgtaccta
      601 atgattccac tagtgaccac gcactaaagg aaaacatttc gtaaaattct ttttttgaaa
      661 aaaaaagttt tggtttccac tctgataact tagttaatat tgcagctgta actaaatttc
      721 ctattataga tccaattact agtgaccaat aacttagtcc aattatcgcc aacggtactg
      781 taataactaa aggtatcaat gcagatatta ttctagcata acacaaaatt ttgtaatcaa
      841 aatttcgtct aaaaattgcc gattgtatac tggaaaaaga agtaattgga agagaaactc
      901 cagctactat aattactaat ccaagtccag aatttccaac taagtgcgct attttattat
      961 taaacacaca aataaataac cataataata aagaaatacc taagttacta gtaaaagcta
     1021 catttgttga tgaaaacatg tgttcttcat tatcaaattc atgctgtact aaaaattttt
     1081 gaaatcctga atcactaaac atatcagcga aactaataac catagttaca gtggctacta
     1141 ctccaaaagc atctggagtc aataatctag ctaaaatcat attcgaaatt ggagttataa
     1201 tctttgcaat tatttcagct aaggatgacc attttaaagc ttttgctgtc tttccatgaa
     1261 tattatttcc catttattac cccattttgc taaataaata tagcaacctt tcgcatatta
     1321 aacattactt tttaatcaaa tttataatca aatttcaact ctgttatgat tgctggattt
     1381 ccatatacta agctatatgg tggaacactt tttgtaacta cagcatttgc acctatgatt
     1441 gaatcatgtc cgatattaat tggtcctaaa acacatgcac cagcgaaaac agccacattt
     1501 tcctctagcg ttggtgcccc aacagtttct tttccattta ttattttgcc atttccaccc
     1561 aaagtcacat tttgatatat tttacaatta ttaccgattt ttgttttagg gtggaataca
     1621 caaccaagac cattatggac tagttgagca ttagagctaa aatctacctg tccgtagaat
     1681 tcacatccat aaataatcct taaaatatag caggatattt ttgacatttt attttgacca
     1741 ttcctgtatt gtctacaagc tttatctgcc catttctctg cttttcttct cattttttct
     1801 acctcaactt tcttttttta tagttctttc tgatgcagaa atcatacaca aaacaactat
     1861 tgcgtaaaga aaacgcgaag atacaatatc cggtataaac attgctccca ctactatcat
     1921 caaaaataac catttatagc aacctgattt ctttaattct tcatacgtaa atttaaagaa
     1981 gccacttaaa ataattagcg taccaataat tcctgtctgt aataaatatt gaataaaaaa
     2041 attatgtggt actgctacac ctagtagcgt gtataaatta tttaatccta atccatatcc
     2101 tattaagtat ttatcactaa aagcatttaa agaggacata tagttttcta atctgcctga
     2161 agaatccaaa agactttgtc cagaacgacc tgccattaac attactagta tataaggtgt
     2221 agttatgatt ccacctatta gaactaaaag atgaggaaca ctaattctat ttatattaaa
     2281 tagataatac aatgtcattg tgacaaacaa ggaaactaac cctgttcttg cactaacaca
     2341 taaaattgca cctaataaaa atacttctat tattataaag aataataatt tccaattttt
     2401 taaatccaca tattttagaa aagcatatag acagcctgta gcaagatata accctgcaaa
     2461 actaaaatct cccatcaaac ctgcatatgc aattctattt aaacccattt ctgcataatg
     2521 gcctattatt acaccagtag ataaaatata tactcgttgc aaatatacct gaaacgccat
     2581 agatactact cctatgaaat agaaattcca cccaatcata gttaagtttt tgctatacat
     2641 tttaaaacgt tttcctatta aaaaactaca gataaacaac gaaatagtca ttaattgctt
     2701 taatgcatct attaaaaaag gtaagaacat agacgtcgat attccaaaac aaataaataa
     2761 aaataaaata atcaagtatg aacgatttaa tgttccttct ataataatat ataacatcaa
     2821 aattat
//
LOCUS       DACDRK010000146         2723 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDRK010000146 DACDRK010000000
VERSION     DACDRK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.150"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWJ77_002712"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWJ77_002712"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9454254.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1067 a          420 c          558 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaa
//
LOCUS       DACDRK010000147         2609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDRK010000147 DACDRK010000000
VERSION     DACDRK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.152"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            102..2543
                     /locus_tag="IWJ77_002713"
     CDS             102..2543
                     /locus_tag="IWJ77_002713"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9454255.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          872 a          461 c          614 g          662 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgctt
//
LOCUS       DACDRK010000148         2600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDRK010000148 DACDRK010000000
VERSION     DACDRK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.153"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            395..601
                     /locus_tag="IWJ77_002714"
     CDS             395..601
                     /locus_tag="IWJ77_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9454256.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            601..852
                     /locus_tag="IWJ77_002715"
     CDS             601..852
                     /locus_tag="IWJ77_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454257.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            867..1304
                     /locus_tag="IWJ77_002716"
     CDS             867..1304
                     /locus_tag="IWJ77_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454258.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            1297..2004
                     /locus_tag="IWJ77_002717"
     CDS             1297..2004
                     /locus_tag="IWJ77_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454259.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            2385..2564
                     /locus_tag="IWJ77_002718"
     CDS             2385..2564
                     /locus_tag="IWJ77_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454260.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT          944 a          440 c          541 g          675 t
ORIGIN      
        1 ttttacatag aaacaggaaa agaccttttt aggaagacac aagtgccttc tcaattgaaa
       61 aacctttctc tcactccaac cgccaccgcc gttcgagaag aaaggaagtg aacgttgcac
      121 gttcactaag atatggtgtc ttgcaaagca agacaaaata gaaaaaactt cctctcacca
      181 aaaggttcgt tcctgttcgc tatctcttta ttgatttgcc agaccggcaa caagttgcct
      241 tttatcacca aaaagcgaca aggacaaggg taaatggcac gctgcgcgcc cttgtccttg
      301 tcgcttttcg ttgattcatt ctggccaaca aatcaataaa gaaagaacta acgaaaggaa
      361 ataaaacctt tcctaagagg aggcagaatc aatcatgcga aagaaatttg attattgggg
      421 tgttcccttc agcgaattat tcccaaacta tcatgcacca catactgtcg aatgcgattg
      481 tggagaaaga gcgaaatgta tcaaaagcta tcgtttgtat caatgtccaa catgcgggaa
      541 aaaatatacc ttgtcttatg gcgattatgt tttgattgat gagaaagtaa agaaacggtg
      601 atgaaaacac aacacaaatt attgactgta aaagaagtcg ttcaaatttt ggtggacaaa
      661 gaagtcattt ctgaaaaagt agcagaagct ttacttattc cgcggtactg gacgatagaa
      721 acccctttag tagaggatgc accgactatg ctatttgaag acctagtgaa gattgcagga
      781 tatgaactgc attatcaaga aaacatggaa acttatgaaa tcgaatattg gttaaccaaa
      841 ttagagaact aaaaaggaga aaaaatatgg ctcaaaatta ctatgacgaa tttgtcaaat
      901 tacctttgga taaaatggca caaaaaatgg aggatatgac gttcctatac catgaaacac
      961 gtgtacccaa aaaacattac aaggaaaaat tgtcagtcgc agtggaagaa atgattgaat
     1021 caggtgtcga aatgaatctc attgcgacgt actatcgaac actggaagaa ctgaaaaaac
     1081 aaaatggaaa atggtttttc caagccttac tatgcttgga agtaggtgtc aaaccaagca
     1141 ctatcaaacc ttccgaatat caagccttag aactgactta tgcaaaattc gcacagatta
     1201 agaaagctaa agcaatggct agcgactggc tagatacttt tgaaaatatc gaaaaaaatg
     1261 gtgcatttta tacgattgaa tcggaggaga aagataatga ataaggtggt ttatcagcta
     1321 acaacagaag atgtttcgca atttttaaca aacaaagaac acttgaatga agtaatcaga
     1381 cggattcaaa aagactttag ttgtatggaa gaaatgaagt actttattca aagagtggaa
     1441 gaacagttgt atatggaaag tgaaacgagt caaacgaagg ttacagtcag ccataatcgt
     1501 ttagacgtgt ataaacgtcg tcctgacaca ttaaaaaaag gagcggagct tctacgacta
     1561 gaaacagaaa aatatatcat gaatgtttac ctagaaagga gtgagctggt cgaaaacttt
     1621 gtcagctaca cgatttgcag gaaacactat tactcagaat cagaatatta ttcaattttt
     1681 gagagcatgg aattaatgga agaagaaatg gaagagctaa acagcaaaga tgcgacggta
     1741 gacgattatt accttgtatt tactagattt ttgacgtgtg ggacggaatc tatcgtgtta
     1801 atgggaatgg attatgaact cactgtaccc gaagcatttt tgagggaata tgtagaagaa
     1861 gtagataacc caggcgacga ctggtacacg actttaactg attttttaga cacctacact
     1921 tacgacgata cagaagaaat ccaaaatgaa cttgaccgta aacgaatgcc atatcaacga
     1981 tttgaaagga aaaaaggtca ataaccattt ataccgaaaa gtgaggatgt gtgaaataag
     2041 aacaaaaaac cttgaaatta agggaatggt tcttatttcg caccaatcga tccaaccgcc
     2101 cccgccgctt aggtggtgcg aaataagcgg gcaaagcccg ataacctaag agaaagaagg
     2161 agatccatat gaataaaatc attcctctat tgttcgtact gtttgttggc gagatactta
     2221 tgcgtcatgg tgcagctcta catttaaact tcgtagtatt cttaggaata ctggtatttt
     2281 gtttgatctt ttggcgcttt gtgttaaccc ttgtgagtct aatattcaag ttgattgcag
     2341 ccatattttt aatagggtta tttcgatgaa aggagagagt actaatgcca attgcaatag
     2401 gaaataaacg gttacccgtg acgttggatg aaaagagaca aaaagaattg cagcaactaa
     2461 agcagaagta cggcaaaagt gaatccagga ttatgtgtat tgcgttagat ttattgattg
     2521 cccaagaaaa agcaggattt gaggtaccag cactcaaaaa gtgacgtcac tttttatcct
     2581 aaaaactaaa agtgatagca
//
LOCUS       DACDRK010000149         2595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDRK010000149 DACDRK010000000
VERSION     DACDRK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.154"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..411)
                     /locus_tag="IWJ77_002719"
     CDS             complement(<1..411)
                     /locus_tag="IWJ77_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454261.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNY"
     gene            complement(1125..1415)
                     /locus_tag="IWJ77_002720"
     CDS             complement(1125..1415)
                     /locus_tag="IWJ77_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454262.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1623..2315)
                     /locus_tag="IWJ77_002721"
     CDS             complement(1623..2315)
                     /locus_tag="IWJ77_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9454263.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          771 a          480 c          365 g          979 t
ORIGIN      
        1 atagttcaag gtggttgctc tccaaccggt ttcccccatt actgtcaatg gtattaaaca
       61 gtatatagag caaaccaata ataaattttt aacatttatt ttctcaaaaa catacttgca
      121 aagtaatact acaattccta acataatcgt agaatttaat aatttccata gcataaagtg
      181 tttagaaaag aacattaaga atagttctat aataattctt gaactccatg tcttataatg
      241 ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc
      301 cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag
      361 taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact
      421 ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa agataactta
      481 aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg
      541 atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg
      601 gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt
      661 taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac
      721 tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta
      781 tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa
      841 ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg
      901 agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat
      961 gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat
     1021 tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt
     1081 ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca
     1141 tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac aattttgggt
     1201 ttccctaaca catgatagga tctcacaaca aattgctttc tattattata ataacctgta
     1261 acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc
     1321 aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta
     1381 cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat accattatac
     1441 gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat
     1501 tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa
     1561 agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact tgttaagaaa
     1621 gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt atcttccact
     1681 tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt ttcagcagtt
     1741 gcttcgtcta atttcttttg tgtatctgtt aactgagttt ctacatttgt tttttcttgt
     1801 gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc tgaaagtttt
     1861 gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat ttcatcattt
     1921 ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg tagttgtgca
     1981 tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg atttttttgg
     2041 ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga atcttgcaat
     2101 gtctgttctt tttgtgctag tcgagcggta agatctgtaa gcgcttgctc tttatctgtc
     2161 aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt ctctccaccc
     2221 cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca tagaaatgca
     2281 acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta atccaatact
     2341 tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa attacatgtg
     2401 taattttaat ataaatacta tcaaatactc atttaatcct ccttttttat ctatttaaaa
     2461 aatctttttt atagagaaaa atgcaggtta atactttctt aaataaatat ttatggtgaa
     2521 caaaaacaaa aaagcaagtt ttattaaacg taaccctttt catttcatag attactgtat
     2581 ttattacaaa aaacg
//
LOCUS       DACDRK010000150         2533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDRK010000150 DACDRK010000000
VERSION     DACDRK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.156"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..379)
                     /locus_tag="IWJ77_002722"
     CDS             complement(<1..379)
                     /locus_tag="IWJ77_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9454264.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            598..1968
                     /locus_tag="IWJ77_002723"
     CDS             598..1968
                     /locus_tag="IWJ77_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9454265.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1989..2273
                     /locus_tag="IWJ77_002724"
     CDS             1989..2273
                     /locus_tag="IWJ77_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454266.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          860 a          380 c          522 g          771 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac ata
//
LOCUS       DACDRK010000151         2454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDRK010000151 DACDRK010000000
VERSION     DACDRK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.157"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWJ77_002725"
     CDS             132..344
                     /locus_tag="IWJ77_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454267.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWJ77_002726"
     CDS             506..1024
                     /locus_tag="IWJ77_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP9454268.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IWJ77_002727"
     CDS             1031..1543
                     /locus_tag="IWJ77_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454269.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="IWJ77_002728"
     CDS             1814..2437
                     /locus_tag="IWJ77_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9454270.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          408 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaa
//
LOCUS       DACDRK010000152         2429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDRK010000152 DACDRK010000000
VERSION     DACDRK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.158"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWJ77_002729"
     CDS             <1..848
                     /locus_tag="IWJ77_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP9454271.1"
                     /translation="ISAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYG
                     TRKLKKCLAKRGLQLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTF
                     TQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIP
                     YSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEF
                     VHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDT
                     SGEAA"
     gene            complement(878..976)
                     /locus_tag="IWJ77_002730"
                     /pseudo
     CDS             complement(878..976)
                     /locus_tag="IWJ77_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
     gene            complement(977..1603)
                     /locus_tag="IWJ77_002731"
                     /pseudo
     CDS             complement(977..1603)
                     /locus_tag="IWJ77_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
     gene            complement(1762..2028)
                     /locus_tag="IWJ77_002732"
     CDS             complement(1762..2028)
                     /locus_tag="IWJ77_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9454272.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(2018..2374)
                     /locus_tag="IWJ77_002733"
     CDS             complement(2018..2374)
                     /locus_tag="IWJ77_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9454273.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          708 a          469 c          403 g          849 t
ORIGIN      
        1 ccatatcagc gatgtgcaaa attctaaata tttctcgtca gacctactat tatcaagcga
       61 aaccgatcga aaatgagtcc gacttagaag agatcgttca ggaagagttt attcgaaacc
      121 gaaaggctta cggtacccga aaattgaaga agtgtttagc aaagcgtggg cttcaactca
      181 gtcggcgccg aatcggtcga atcatgaaac gccgcggatt gacatctacc tatacgatcg
      241 ctcattttaa agggcaacga acagcttgta atgaagcgaa aacagcgaat gtattagatc
      301 ggacctttac acaagaacag ccattggaag ccatcgttac ggatcttact tatgttcgcg
      361 tggggaaaaa gtggcattat atctgcttaa tacttgattt gtttaatcga gaaattattg
      421 gttattcctg tggtgagaag aaagatgcct cattggtaaa agaagccttt ggacggatac
      481 cgtattcttt aacagacgtc aagctttttc atacagaccg gggaaaggaa tttgataacc
      541 aaaccattca tgagattctg aatggttttg gaattactcg ttcattgagt aggaagggtt
      601 gtccgtatga taatgccgtt gtggaatcaa cctataaatc tgtcaaagta gaattcgtgc
      661 atcaatacca atttgagaca ctggcacagc tacgtctaga attgtttgat tatgtgcatt
      721 ggtggaacta tctacgctta catggcacgt tggcgtatga aacaccgatc caaattcgac
      781 aacagagatt ggcgaagcga atccttgata atgagcgcgg atctgatacc tctggagagg
      841 cagcgtaatt gaatgattgt gcttctgccg gagaaaatca gatccgagga tgctcattgt
      901 caagggcaat cgtagccata acaccgcagc attcacaaca ccttataatt tttgtcaaaa
      961 aaagtgttgc cattccagga tcaaaaatat ctaagttgtc atcaaaatct ttatcagttc
     1021 cggtaacttt tttgttaatt actgaaggtg tcttgtaaga aatagaatct aaatcaaagc
     1081 gttttgattc taaattattt tgtgctttct caattaaata attcactttt tttgtgagaa
     1141 accttctgat attgtctttg ctatatgttc cataaaaacc cttatcttct aaagagtccc
     1201 tgtacttctt aacttctata tactcttcag ccaaaacttg tggaactggt tcatctaaga
     1261 aaaaaacccc tacctcgcct ttgctatttt tataaaagta atcaatttct gctatagttc
     1321 ccgatctata attatcaact ggagaaccca acttatggcc aaaaatcgct aataatatat
     1381 cactattttc tacaatttgt ttattaataa cactttgccc agagctatta tttatataag
     1441 taggaatcgc attcgtttcc catcgaattg gcatcaaaat tacttttgta ttatctgtat
     1501 tttctatatt ccatctatat atggtttctt ctacttcatt cctctttgct gctatatcac
     1561 ctggtgatgc tataaatact tttattagat ttatggtttt catttattgt acctttctct
     1621 ttatgtctct ttaaagtaat ttttgtataa aatttattga cttttacact ataggacaac
     1681 ttttatctat ttagtacaaa aaggcccact aaactaattg attagttagg agcctattac
     1741 aggtatattt tcaactcgct tttaagcaaa ttctactacc agctttttgc ctaatgccct
     1801 agcgatatca tcaagtttat cgaaagatac gttatcccct ctctcaattc gagcaatagt
     1861 agattgagtt gtatgtgctt tttgtgcaag ctccgcttga gttaggcctg cttcctcccg
     1921 tgcatgataa agagcaaccg cagtttcgag ttttcttcct tcacggtcat atgcttctgc
     1981 aaaatctttg tctttttgct tttcttcaat caatttttta atcatcattt gtgaaccact
     2041 cctctctaag ttcttttgca tgtttaagtt cacttattgg tgttttctcc gtttttttag
     2101 taaatccatg ggtaatgaca taccttccat tgaccacatg gaaatagata gctctttgga
     2161 tgtttgatcc taccttactt cttaattcgt acaaattagt gtctaatttt ttcacccatt
     2221 tcaaccgttg agctactagc atcccattct tttctgtttc ttctattgta cgtaaaagct
     2281 tcgcactatc tttttttggt aatgtttcca accattctaa aaattcatca tgaccattag
     2341 gtcttttaaa tgtttcaaaa gatatttttt ccatacccaa ttatatcata tattgatttt
     2401 atattatagt atataacata aaaattcga
//
LOCUS       DACDRK010000153         2406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDRK010000153 DACDRK010000000
VERSION     DACDRK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.159"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWJ77_002734"
     CDS             5..1150
                     /locus_tag="IWJ77_002734"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9454274.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWJ77_002735"
     CDS             1321..1779
                     /locus_tag="IWJ77_002735"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9454275.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2406
                     /locus_tag="IWJ77_002736"
     CDS             1938..>2406
                     /locus_tag="IWJ77_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9454276.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          801 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttt
//
LOCUS       DACDRK010000154         2406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDRK010000154 DACDRK010000000
VERSION     DACDRK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.160"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..103)
                     /locus_tag="IWJ77_002737"
     CDS             complement(<1..103)
                     /locus_tag="IWJ77_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9454277.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(177..458)
                     /locus_tag="IWJ77_002738"
                     /pseudo
     CDS             complement(177..458)
                     /locus_tag="IWJ77_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            920..2302
                     /locus_tag="IWJ77_002739"
                     /pseudo
     CDS             920..2302
                     /locus_tag="IWJ77_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          719 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc cttttttttg
     2341 taaaaaacta ttgcacaata caagtagcct acggctatcg aaatttccat aactttcgag
     2401 caagaa
//
LOCUS       DACDRK010000155         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDRK010000155 DACDRK010000000
VERSION     DACDRK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.161"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWJ77_002740"
     CDS             207..2069
                     /locus_tag="IWJ77_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9454278.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IWJ77_002741"
     CDS             2069..>2400
                     /locus_tag="IWJ77_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9454279.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDRK010000156         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDRK010000156 DACDRK010000000
VERSION     DACDRK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.162"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWJ77_002742"
     CDS             <1..378
                     /locus_tag="IWJ77_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9454280.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWJ77_002743"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWJ77_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9454281.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="IWJ77_002744"
     CDS             1859..2026
                     /locus_tag="IWJ77_002744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454282.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="IWJ77_002745"
     CDS             2080..>2232
                     /locus_tag="IWJ77_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9454283.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DACDRK010000157         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDRK010000157 DACDRK010000000
VERSION     DACDRK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.163"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IWJ77_002746"
     CDS             <1..544
                     /locus_tag="IWJ77_002746"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9454284.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="IWJ77_002747"
     CDS             complement(900..1472)
                     /locus_tag="IWJ77_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9454285.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="IWJ77_002748"
     CDS             complement(1488..2093)
                     /locus_tag="IWJ77_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9454286.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DACDRK010000158         2151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDRK010000158 DACDRK010000000
VERSION     DACDRK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.164"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..286)
                     /locus_tag="IWJ77_002749"
                     /pseudo
     CDS             complement(1..286)
                     /locus_tag="IWJ77_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(608..829)
                     /locus_tag="IWJ77_002750"
     CDS             complement(608..829)
                     /locus_tag="IWJ77_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454287.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(927..1172)
                     /locus_tag="IWJ77_002751"
     CDS             complement(927..1172)
                     /locus_tag="IWJ77_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454288.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1412..2026
                     /locus_tag="IWJ77_002752"
                     /pseudo
     CDS             1412..2026
                     /locus_tag="IWJ77_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          707 a          386 c          317 g          741 t
ORIGIN      
        1 ccgcatcaat tgcacgataa agataatgcc aacgtccctt aattttgata taggtttcgt
       61 ccattttcca tgaatagaag gattgtctat ttttcttctt ccaaagataa tagaggactt
      121 tgctgtactc ttatacccaa cgataaatcg tagtatgaca aacatttatt ccacgatcat
      181 ataacaattc ctgaacttca cgatagctta gattgtaacg caggtagtaa ccaacagcga
      241 caataatgac gtcttttttg aattgtttgc ctttaaaatg attcattact ctgtcctctc
      301 tgtctttttt ctcaatttta cactaaaata gattttttgg aaaactttgc aacagaaccg
      361 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
      421 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      481 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      541 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      601 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      661 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      721 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      781 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      841 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      901 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      961 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
     1021 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
     1081 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
     1141 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
     1201 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
     1261 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
     1321 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1381 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1441 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1501 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1561 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1621 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1681 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1741 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1801 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1861 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1921 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1981 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     2041 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     2101 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt g
//
LOCUS       DACDRK010000159         2059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDRK010000159 DACDRK010000000
VERSION     DACDRK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.165"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..223)
                     /locus_tag="IWJ77_002753"
     CDS             complement(<1..223)
                     /locus_tag="IWJ77_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454289.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLA"
     gene            537..1151
                     /locus_tag="IWJ77_002754"
     CDS             537..1151
                     /locus_tag="IWJ77_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9454290.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1175..1525)
                     /locus_tag="IWJ77_002755"
     CDS             complement(1175..1525)
                     /locus_tag="IWJ77_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9454291.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1519..1782)
                     /locus_tag="IWJ77_002756"
     CDS             complement(1519..1782)
                     /locus_tag="IWJ77_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9454292.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          711 a          347 c          307 g          694 t
ORIGIN      
        1 gagcaagtaa tggagaaact attaaataat tttcagtaac agttaagtcg ttagttgggt
       61 taccaatggc tattccgttc ctatctaaag gagttaattc ttggttatta tctactacag
      121 tagttggttc atttgtacta tcagcaaatg taggaattgt agttccagtt acactaattc
      181 caatagttaa aatacctaaa gataaaataa acttattttt cattgtctat cacctcgaaa
      241 tcaaaaataa gaaaacgttt tcttaataaa tcttaacaga taaatcaagt tatatactag
      301 aaaattcata aaatatattt ttaatgtcat aatcgtttaa attttcttga ttgacattta
      361 tttacaaaaa atattgccat ttaaaaagaa aagtgacaca tactatttcc tccatcatta
      421 gtttttgtct atataaggta taccctaact agacacgacc gcgacattga aaaatctggt
      481 tttctcgtgt atgattaggg tgttttttaa atagttgcag aaagtaggta aaaattttga
      541 gagttgcata catacgtgtc tcatccatcg atcaaaatga acaacggcag attgaagaaa
      601 tgaaaaaatt cggtgcagag agaattttca ttgagaaaca atctggcgca acgattactc
      661 atcgacctgt ttttcaagaa gcactggatt tcgtccgaga tcaagatatt tttattgtgg
      721 aagctattga tcgacttgga agaaattacg acgagatcat cgcttcagtc aattacttga
      781 agaagaaaaa tgtgcagctg atcatcacaa gtctaccgat catggccgaa gcaattggca
      841 atcctctcct ggataaattc attaaagatt tgatcattca aattttggcc atgatcgctg
      901 aacaagaacg taccgaatct aaacgacgtc aggcacaagg cattaagatt gcaaaagcga
      961 acggtgttta taaaggacgt cctaagctat acagtgccaa tgcaaaagat ccccaacgtc
     1021 gtcttgttta taaaaacatc gttgaggatc taaaaaaagg tgttgcgatc gctaagattg
     1081 caaaagacta caatgttacg agacaaaccg tatatagaat aaaaaaagac agcatggtca
     1141 atcacgaata gttgatcatg ctgctgtttc tattttattt gcttactttt tgaaaaactg
     1201 ttctaacttt catcaataac tcctcaacta aactatcatg cgctttttcc aaaaaaatac
     1261 actctctggc ttcataatca attgtcgtta gttgatctaa taatactttt cctgttgtct
     1321 tcatttcata ttctgagttt atatttacat atagcggata gtttctagtt gtactagaaa
     1381 ttggagcaac tgtaacaacg ttactataat ccgcaataat atcatgactt aaaacaatat
     1441 aaggtctttt acctgtctgt tcgtgtccag atcgaggtgc tgtattcaat aatagaatat
     1501 cgccttgtct aggttttctc accatttttc attacctact gcttcaccta aattttgaat
     1561 cgtcacatga tttctttcat cagtgtaatt ttcaaataac tgttcaattg ttaattcatt
     1621 ttctatatta gccgcttcga taattagccg cttctgaccg tttacttctt cttgtattat
     1681 cttcactttg tcttctgtat gaattccaag atattccata atatactttg gcaatctaag
     1741 gccattacta tttccccatt tttttatagg taacatttcc atagtagaca cctctttctg
     1801 tatatacata gtataacata cataagaaaa aacgtatata cttaattatc cttttctatt
     1861 aaattagatt aacattccat gaatccataa actatgttaa catttatatg aaaatagtcg
     1921 aaaggaaatc ataatatgtt taagaaaaca aaatctttag aatcaggtaa gaaaaaaatt
     1981 gatctaaaaa taatctcttc tggatacttt aaagatttca tagttattga tacaaaatgg
     2041 aataaaaagg acatagaaa
//
LOCUS       DACDRK010000160         2059 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDRK010000160 DACDRK010000000
VERSION     DACDRK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.166"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..337)
                     /locus_tag="IWJ77_002757"
     CDS             complement(<1..337)
                     /locus_tag="IWJ77_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9454293.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="IWJ77_002758"
     CDS             complement(404..559)
                     /locus_tag="IWJ77_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9454294.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1177..1398
                     /locus_tag="IWJ77_002759"
     CDS             1177..1398
                     /locus_tag="IWJ77_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454295.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1399..1656
                     /locus_tag="IWJ77_002760"
     CDS             1399..1656
                     /locus_tag="IWJ77_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454296.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1792..>2059)
                     /locus_tag="IWJ77_002761"
     CDS             complement(1792..>2059)
                     /locus_tag="IWJ77_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9454297.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          679 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aaacgctgaa acccttggga gagtaaggct
      721 aaacagctat cgaccaatga ccaatgacca ataagggggg tgtcgtagtc cccccctcct
      781 ccgcccaatt cataaacacc aaaaccaaaa aacaaacctc agggctccgc cccgaacccc
      841 gacctccttc taccgaaata tatgcaggcg ccacgcttac tatttcgttc agctatcggc
      901 ttttgcaatc taacacgacc gtttcgctac cagtcgcctt acaggctcca ttctcgcttc
      961 tcttacgtgt tttaacgatt gcgccgataa ctaaacgaaa tagacgctaa atcgcttaaa
     1021 acgcaaaata gacccttttt agagcattga ttttttctac tagtcatttc gataatataa
     1081 aattgaccac aacacagaaa acttatatta taagcttatg tcgtaagtcc gacgtactca
     1141 cgtaatatag acggacgtta aatggaagga gcataaatga gagtgataaa atcagaaaaa
     1201 ttcactttgg ccgaaattat gacgaccagc gaacttgcac aagaacttga ttacaaccaa
     1261 gcttacgttt tgcgcttagc gaaagaacat ctgactgaag gaaaagaata tcgctctgcc
     1321 ggtagaagaa actatctttt cagcccagaa gcccttaaaa aactagaaaa agtcataacc
     1381 accaaagaag gtgagtaaat gcctaaagaa acaactatca acgttgaaca actagaaaaa
     1441 ttgctagaat catattccaa tgattttatc gaattgaccg gcggaaaagt cgaagaaacc
     1501 accgacctct ttgacctact tctcacaaat attctggaat taaactctga agaacaaaaa
     1561 gagcataagg gaaaactgat agcaccttcc tacaggaagg tggctaagtc ggctgtgcca
     1621 actggttttc ttacaaaaca accttatatt ttttgatatc acaaaaaaat ccattttgga
     1681 cctattttcg gtcataatat agtacctact tttggtcata gtttcgtccg tagttttggt
     1741 catagtgact aagactttca cgcacaaaaa aagagctgaa attaacagct cttatccttt
     1801 atttattttt gaatttacac ttccaccatg acgattcgat tttatatatt gcccgataat
     1861 ataagctgaa ttatcacaaa gaagcggaaa ccaatcatca aatctttctg acattgcata
     1921 gtcaattaaa tctttgattt cagtgatatt cttagaatca acaaacgaca tcatctctct
     1981 aatcaactca tatcgttctg cactcgttac tgacaaataa ctagcaattt ctgcaccacc
     2041 atgagcgata atatcactt
//
LOCUS       DACDRK010000161         1981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDRK010000161 DACDRK010000000
VERSION     DACDRK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.167"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            272..838
                     /gene="amaP"
                     /locus_tag="IWJ77_002762"
     CDS             272..838
                     /gene="amaP"
                     /locus_tag="IWJ77_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9454298.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            851..1039
                     /locus_tag="IWJ77_002763"
     CDS             851..1039
                     /locus_tag="IWJ77_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9454299.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1052..1609
                     /locus_tag="IWJ77_002764"
     CDS             1052..1609
                     /locus_tag="IWJ77_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9454300.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1641..1883
                     /locus_tag="IWJ77_002765"
     CDS             1641..1883
                     /locus_tag="IWJ77_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9454301.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          722 a          305 c          406 g          548 t
ORIGIN      
        1 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
       61 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      121 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      181 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      241 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      301 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      361 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      421 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      481 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      541 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      601 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      661 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      721 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      781 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      841 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      901 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
      961 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1021 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1081 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1141 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1201 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1261 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1321 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1381 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1441 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1501 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1561 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1621 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1681 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1741 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1801 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1861 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1921 aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta tatgaagtat
     1981 t
//
LOCUS       DACDRK010000162         1908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDRK010000162 DACDRK010000000
VERSION     DACDRK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.168"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            60..586
                     /locus_tag="IWJ77_002766"
                     /pseudo
     CDS             60..586
                     /locus_tag="IWJ77_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            741..1316
                     /locus_tag="IWJ77_002767"
     CDS             741..1316
                     /locus_tag="IWJ77_002767"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9454302.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1359..1556)
                     /locus_tag="IWJ77_002768"
     CDS             complement(1359..1556)
                     /locus_tag="IWJ77_002768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454303.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            1543..1725
                     /locus_tag="IWJ77_002769"
                     /pseudo
     CDS             1543..1725
                     /locus_tag="IWJ77_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          675 a          296 c          366 g          571 t
ORIGIN      
        1 gaagtccctt ctgtagaata gagttaccac aacacattca cagaaaagag gacttccata
       61 tgaacgattt tactacagaa atcctaaaga ctctagcgaa caaaggcgat ttgaatggat
      121 tattccgtgt ccatttggaa aaagctgtca atacgcttct caaaacggag ttaacggctt
      181 tcctcgatta cgaaaagtac gatcgcattg gttttaacac gggtaattct cgtaacggct
      241 cctatgaccg tacggtcaag accgagtatg gggaacttca tctccagatt ccgcgcgacc
      301 gcaatggcga gttcaagcaa cagactgttc ctgcttatag acggacgaat gacacattag
      361 aggagaccgt cattcacctc ttccgaaaag gtattaccat gtcggaaatc gcagacttga
      421 ttgagaaaat gtatgggcat cactacacgc cccaaaccat gtccaacatg actaaagtgc
      481 tgactgaaga agtaaatgca tttaaagtta gaactctaaa tgataagtat gtcgctattt
      541 tcatgattcc ttcagttttt cagatgatat accactcaaa aaacaatatg agagatgttc
      601 aggaatacca tctctaatgg cttataaatt ggttttaggg atagtagatg ttatagacat
      661 atcaaggaat aaacgagcag ctgcataaaa gcagatggaa ttaaggaata attgaattgt
      721 aaaagaaggg aataaattta atggcacaat tattttacag atatggaaca atgaatagtg
      781 gaaagagtat tgagctactg aaagtggctc ataattatga agaacaaggg aagagagtta
      841 tcataatgac aagcgcaata gatactcgat caggatttgg aaaagttgag agtcgtatag
      901 gtgtttcgaa accagctatt gcaattactt ctgaaatgga tgtgtacgga gaattagtgg
      961 agattattga tcagtacgga aaagttgatt gtattttggt cgatgagtct caatttttaa
     1021 gtagagagaa tattattgat ctatcactta tcgtaatgaa tgaggaagta ccagtaattt
     1081 gctatggatt gaagaatgat tttcaaaata atctcttcaa gggatccgaa gcattattga
     1141 catttgctga taaaattgag gaaattaaaa ctatttgcca tttttgcaat aaaaaggctg
     1201 taatgaatgc tcgtattgtt gatggtattc cagtttattc aggtgaacag attgtagtag
     1261 gaggtaatga agattacctt tcggtatgtc gcaaccacta ctacaacgaa gattgaaata
     1321 aaaaacgtgt aatcaaataa ttgattacac gttttttatt attctgttaa aggttgagga
     1381 tcagaaattt ttaggtatct ataatcctca cctaaaaatc cgttaaaacg aagtaaaaca
     1441 tcatgaccat ttaaatcagt tggaactaaa attggaatta atttatcaac agtagttttg
     1501 ttgtaaagtt caaaagtgtt ctccattcct tctggttcta caatcgaatc tttcaataaa
     1561 caaatcaaga aatacagccg cagaaaagag cagtttcaaa atgaagaatc actagagcgt
     1621 ttcctagtat ccatctttga tacatacaat caaaaattct taaatagaag ccataaaggc
     1681 ttccaacaag tgacggatac attagcttca atgtttactg agtaacccat tattttgcag
     1741 aaggacgagt tatttacaca aaattattga cgctccctgt ttggagtggt aaaaagattg
     1801 agaaattgtt tcattagcta ttacgattat tattgtaata tatttttatt gaaaatcaaa
     1861 attaaaataa ttcactgtaa aaacaggatt attttttcta ttttggta
//
LOCUS       DACDRK010000163         1796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDRK010000163 DACDRK010000000
VERSION     DACDRK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.169"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWJ77_002770"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWJ77_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9454304.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACDRK010000164         1783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDRK010000164 DACDRK010000000
VERSION     DACDRK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.170"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..542
                     /locus_tag="IWJ77_002771"
     CDS             144..542
                     /locus_tag="IWJ77_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9454305.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="IWJ77_002772"
     CDS             526..1221
                     /locus_tag="IWJ77_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9454306.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          677 a          260 c          344 g          502 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 ttatttctca tttaaacgat gtattcccta acctctaata catccttgta actctgaagt
     1381 tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata aaaatatctt
     1441 tcgtgtttct ctagtataac agaataagag gaacaagtcc agaagggata tttttagtct
     1501 tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg aagcgatacg
     1561 gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg attcatgtga
     1621 tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc agtgatttgt
     1681 tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt gcctcaaaaa
     1741 taagaaggaa tttccaaaaa tcacttctca aattttgcca cat
//
LOCUS       DACDRK010000165         1748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDRK010000165 DACDRK010000000
VERSION     DACDRK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.171"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1146
                     /locus_tag="IWJ77_002773"
     CDS             <1..1146
                     /locus_tag="IWJ77_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9454307.1"
                     /translation="DIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERA
                     KNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGD
                     KIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSAAQELYENTLITDIHTAAKV
                     YGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISG
                     IYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVF
                     ISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTL
                     SSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            1276..1608
                     /locus_tag="IWJ77_002774"
                     /pseudo
     CDS             1276..1608
                     /locus_tag="IWJ77_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          655 a          240 c          315 g          538 t
ORIGIN      
        1 gatattaaaa agatatctca aacagatagt atagactcct atgtaaaaag aataaacagt
       61 gttgcagatt tagttgatta tgatattatt gaaactcaag agactcttgc gaatcaatca
      121 cctgaaagag caaagaattt taaaagaaca gttatgttaa ctggggttaa cgactcaaca
      181 aaagaaacaa aatttgtatc agaagcatat aaattagtag aaggaaagca tttagaaaat
      241 gaagataaaa ataaaatctt aatgcataaa gatttagctg aaaaaaataa ccttaaagtt
      301 ggagataaga taaaaataaa atctaattta tttgatgctg ataatgagaa aggtgcagat
      361 gaaacagtag aagtagaaat taaaggtcta ttcgatgggc ataatagcgg tgtagtaagt
      421 gcagcacaag aactatacga aaatacatta atcactgata ttcatacagc tgctaaagtt
      481 tatggtaata ccgaagatac agctgtatac caagatgcaa cattctttgt aaaaggtgat
      541 aagaatcttg atagtgtaat aaaagatctt gggaaattag atataaattg gagagaatat
      601 aatttgatta aaagttcatc aaattaccct gcattacaac aatctatatc aggtatttac
      661 tcaatttcaa ataaattatt ttttggctca ttaatatttg caggagttgt agtttcatta
      721 ttattattct tatggatgaa tgctagaaag aaagaaatag cagtattact atcattaggt
      781 atatcaaaat tagaaatttt tggtcaattc ctaattgaga tggtatttat atcaatcccc
      841 gcattcgttg gttcctattt cttagctcaa tatacagctg acaagcttgg gaataatata
      901 ttgaataagg ttactggtga tatagctaaa caaatagcta aacaatctgc atctagccaa
      961 ttaggtggtg gggcagaagc tgaaggattt aacaagacat tatcaagttt agatatcaat
     1021 gtattaccta aattcatgat ttatgtagtt atatttatga gtttagtact tcttgtatca
     1081 ttgattattt cttcattcaa tatattaaga aggaatccaa aagaattatt aattgacaat
     1141 aattaaaatt ttcggtgctt tttagcacca aaaatgagag tgtaatataa accgtgtaag
     1201 taaccatcaa tcctaggcgg gtggttgctt cacggttttt atttttctaa aataagatca
     1261 agaggtgatt acacgatggc tagaaagaaa agaaatcctg atgctgagaa gttagctgaa
     1321 tccattttga atacttatca acccgaatct gtagaggata tgcaagatgt tttaaaagat
     1381 gtgtttggtc ccttatttga aaaaatgctt caaggagaat tgaataacca tttagggtat
     1441 gacgcccatt ctaaagagcc aaagaaacat gacaatcgac gaaacggtta tggcaataag
     1501 acgcttaaaa cgagctttgg taaagtaaac attgatgttc ccagagaccg ggaggcttct
     1561 tttgaacctg agttgattcc taatagaaaa cgagatgtct ccgacatcgc agactgtaga
     1621 caaaagctgt tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg
     1681 aatttttgaa caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt
     1741 taaattca
//
LOCUS       DACDRK010000166         1642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDRK010000166 DACDRK010000000
VERSION     DACDRK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.172"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..817
                     /locus_tag="IWJ77_002775"
     CDS             5..817
                     /locus_tag="IWJ77_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9454308.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            814..1614
                     /locus_tag="IWJ77_002776"
     CDS             814..1614
                     /locus_tag="IWJ77_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9454309.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
BASE COUNT          492 a          266 c          376 g          508 t
ORIGIN      
        1 aagaatgtta cgtttttttc cgcaaaaaaa caaatatatt caagaaaaaa gcgaatttca
       61 atttaatacc gatggaccaa ttcctcagca cattgccatc atcatggacg gcaatggtcg
      121 ttgggcgcaa aaccgtcgtt tgccacgagt tgctggccat aaagaaggca tggaaacagt
      181 aaagaaagtc acaaaaaaag catcaaggct tggagtgaaa gtgttgacgc tttatgcctt
      241 ttcgactgaa aactggaagc gaccaaaaga tgaagtaagt tttctcatgc aactacctgt
      301 cgacttcttt gatacgttcg taccagaatt gattaaagaa aatgtcaaag ttcatgtcat
      361 gggttatgaa aacgtattgc cagaacatac gcaagatgct gtaagacgtg caattgagca
      421 aacaaaaaac aatactggaa tggtattgaa ttttgctttg aattatggaa gcagagcaga
      481 gatcgtgact gctgtccaag aaattgccga agaagtagca aaaggagaaa tacacgcaga
      541 agaaatcgat gatgaattga ttgctaagca tttaatgaca ggtttcttgc ctaaagagtt
      601 acaagatcct gagctgatga tccgtacaag tggagaagag cgaattagta attttctgtt
      661 atggcagatc gcatatagtg agctgtattt tacaaaagcg ctttggcctg attttgatgg
      721 tgcgcatctt gaagaagcaa tcgcctctta tcaaaatcga gaccgtcgat tcggcggtgt
      781 aaaaaaaaca gaagatgaga aaggggatca gtcatgaaac aacgtgtggt cacagcagcc
      841 gtggcgctag tcttgtttat tccgattata tggatcggag gcatagcggt tgagcttaca
      901 gcagcaatct tagcagtaat aggagtttac gagctgtttc ggatgaaagg attgacactc
      961 ttaagttttg aaggagtttt atccgccgtt ggtgcagtgt tacttgtttt gccaaaagaa
     1021 agatggtttt tctttcttcc agagaatgta agtacattta tgttatttta tcttatagtt
     1081 atgattcttt taggaacatc agttatttct aaaaatacgt atacgattga tgaagcaggt
     1141 tttcctgtgc ttgtcagctt gtatgtggga ataggtttcc agaattttgt caatgcacga
     1201 acagaaagct tggctgtttt gatatttggt ctatttattg tttgggcaac tgatatcgga
     1261 gcatatatgg ttggtcgacg ttatggtcag cataagttaa gtcctgatat ttcgcccaac
     1321 aaaacgattg aaggtgcgct cggtggtatt ggtagtgctg ttgtagtcgc atttctttat
     1381 ttattgctat acccagcaaa agaactattt aatcatggta tgtttgtcat gcttctgatg
     1441 acgattattt tttcagcagt cggacaattt ggtgatttgg tcgaatctgc aatcaaacgt
     1501 cattatgatg tgaaagactc gggaaatatc ttaccaggcc atggtggaat cttagaccgt
     1561 ttcgacagtt tgctgtttgt atttcctatc atgcatttat ttggcattat ttagtaagta
     1621 tacctgcgtt tctttgtgaa tt
//
LOCUS       DACDRK010000167         1641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDRK010000167 DACDRK010000000
VERSION     DACDRK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.173"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1400
                     /locus_tag="IWJ77_002777"
     CDS             <1..1400
                     /locus_tag="IWJ77_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9454310.1"
                     /translation="GEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGN
                     YEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLP
                     ALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYN
                     PEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGS
                     SDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEK
                     VDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEP
                     NAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSS
                     NKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLG
                     TLGLLGYKNRKKENE"
BASE COUNT          649 a          248 c          352 g          392 t
ORIGIN      
        1 aaggcgaaaa agtagaagtt gggggcacag tagatctaac agataatgtt actaacttgc
       61 cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca attgatacca
      121 acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt acgaaagata
      181 cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa tatgaaccaa
      241 cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat aatgttacta
      301 acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc ggtacaattg
      361 ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca gacggtacga
      421 aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca gataaataca
      481 atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca tctgagggaa
      541 ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag aaagtagatg
      601 ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat ggatcatctg
      661 atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat aaatataatc
      721 ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct gagggaatta
      781 agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa gtagatgtta
      841 gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga tctaaagaag
      901 aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct caagttgatc
      961 ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca gaggtgacag
     1021 ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt aattttacag
     1081 ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact gatgaatctg
     1141 gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac agtggaaaaa
     1201 acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa gacaaaaata
     1261 gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac agtaatatct
     1321 ttactattag cggtggttta atactgttag gaacgttagg attattaggg tataaaaatc
     1381 gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa gaggaagaat
     1441 ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta aagatgaata
     1501 tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat tattggttca
     1561 aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa ttatgaagtt
     1621 attctaatcg ataaaaaatc a
//
LOCUS       DACDRK010000168         1639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDRK010000168 DACDRK010000000
VERSION     DACDRK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.174"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWJ77_002778"
     CDS             <1..153
                     /locus_tag="IWJ77_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9454311.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IWJ77_002779"
     CDS             159..575
                     /locus_tag="IWJ77_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454312.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IWJ77_002780"
     CDS             681..944
                     /locus_tag="IWJ77_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9454313.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="IWJ77_002781"
     CDS             944..1216
                     /locus_tag="IWJ77_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9454314.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="IWJ77_002782"
     CDS             1293..1622
                     /locus_tag="IWJ77_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9454315.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          625 a          207 c          324 g          483 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaac
//
LOCUS       DACDRK010000169         1572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDRK010000169 DACDRK010000000
VERSION     DACDRK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.175"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..317)
                     /locus_tag="IWJ77_002783"
     CDS             complement(<1..317)
                     /locus_tag="IWJ77_002783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9454316.1"
                     /translation="MKKIKIMHLLQSNRFSGAENIVCQIMSLFQNNEKFEMIYVSPDG
                     PIANILNIRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSH
                     LHN"
     gene            complement(340..1485)
                     /locus_tag="IWJ77_002784"
     CDS             complement(340..1485)
                     /locus_tag="IWJ77_002784"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9454317.1"
                     /translation="MKLLYILNVANKVNNFSKASMMAAKQTGLEFYIAGNWKYKSDEE
                     RKADEVKYGIKIYQVDFYRNPLNLKNIKAYHQIVEIIKNEKIDIIHCNTPIGGVVGRI
                     AGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPMERWLAHYTDVLITINHEDYTLAQK
                     LRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKELNIPDTAKIILSVGELNENK
                     NHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANELGLHQRVFLLGYRSDIPKLL
                     AAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEI
                     AGAINRLADNKLLCESMGAHNLDKIKDFDLSVVINQINNVYHEIVSK"
BASE COUNT          496 a          283 c          184 g          609 t
ORIGIN      
        1 ttattgtgca aatgggaaag gatgatttgc ttcttatatc tacttgctct tacactagca
       61 ttaaaatcat gagcatgaat tatatctggg tttaattcat gtacggctct gtcaatttct
      121 tttctcgaaa atttttttaa tggtaagtaa cttaaacctc taatatttaa aatatttgct
      181 ataggaccat ctgggctaac gtaaatcatc tcaaattttt cgttattttg aaataaactc
      241 attatttgac aaacaatatt ttcagcgcca gaaaatctat tactttgcaa caaatgcata
      301 atttttattt ttttcatatt aatatcactt tcaattcaat tatttactta caatttcatg
      361 gtaaacatta tttatttggt ttatcacaac tgataaatca aaatctttta tcttgtctaa
      421 attatgtgcc cccatagatt cacacaataa tttgttatca gccaatctat ttatcgcacc
      481 agcaatttct tcttcgtctt taggattgat tagaaatcca cccttaccat cttctataag
      541 atctacattt cctcttatat taccgacaat acatggtaaa ccactagcca tcgcttccat
      601 taaagaggcg ttgaggcctt cccttaatga gggtagaata tatatgtttg cagcagctaa
      661 taatttgggt atatcacttc tatatcctag taaaaaaact cgctgatgta atcctaattc
      721 attagcaata ttttgtagtg tctccctcat ttctcctacc ccagcgataa tataatagat
      781 atctgtatgc agcggaattt tttgtaatgc atgtattact gataaatggt ttttattctc
      841 atttagctct cctacagata aaatgatttt tgctgtatca ggtatattaa gttctttaca
      901 tatactttct ctaaaattcc ttattgatgt ggaagacata aacctatttg tatctatacc
      961 cacacctgga atataataag cttttccacc tttcctaaga cgaagttttt gagctaaagt
     1021 ataatcctca tgatttattg taatcaaaac atctgtataa tgagctagcc aacgttccat
     1081 aggataatat ataaaccaat ttattttagg agcacctgaa taaaaatgaa atccatgtgc
     1141 ctggtaaatt acttttttga ctttacattt aagaccagca atccgtccga caacacctcc
     1201 aataggtgta ttacaatgga taatatctat tttttcattt tttataatct ctacaatctg
     1261 atgataagct tttatatttt ttaaatttag tgggtttcta taaaaatcaa cttggtatat
     1321 ttttattcca tatttcactt catcagcttt tctttcctca tcggatttgt atttccagtt
     1381 accagctata taaaattcta aacccgtttg tttagctgcc atcatactag ctttagaaaa
     1441 gttatttact ttattagcta catttaaaat atacaataat ttcataatta attcttctcc
     1501 atataaaacc gatattcatt cataattctt ccttcattag tagaatattt acgttctaaa
     1561 atgaactcat tc
//
LOCUS       DACDRK010000170         1474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDRK010000170 DACDRK010000000
VERSION     DACDRK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.177"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..365
                     /locus_tag="IWJ77_002785"
     CDS             <1..365
                     /locus_tag="IWJ77_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9454318.1"
                     /translation="LKNLDKDVYFSVVSARRNPYFNMVKRIENEVTIVNKSRYTSRQQ
                     TPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEEDFKWLEFLFPK
                     FLEDSSELQDIYNYLYRR"
     gene            370..1317
                     /locus_tag="IWJ77_002786"
     CDS             370..1317
                     /locus_tag="IWJ77_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9454319.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          501 a          206 c          300 g          467 t
ORIGIN      
        1 atctaaaaaa tttagataaa gacgtctact tttcagttgt ttctgcaaga cgtaatccct
       61 attttaatat ggtaaaaaga attgaaaatg aagttactat agttaataaa tcaagataca
      121 catctaggca acagacacct gaagtttttg aaatgaatgc ctcaatgtat tcttttaaac
      181 ctgaatttct aaaaaaaagt aattatattt ttgatgggca atgtgggata ataaaaatga
      241 gagattatct aattttagat attgatagtg aagaagattt taaatggtta gaatttttgt
      301 ttcctaaatt tttagaagat tcttcggagc ttcaagatat ctataattat ttatatcgga
      361 ggtaaaagga tgagctgtta tcttatcacc ggtggggctg gcttcattgg ttctacctta
      421 gccaatcatt taggagaaga acatgtggta atcgtcgtcg atgacttatc aatgggaaaa
      481 gtagaaaatc ttgatatgac gaaaaatatc acttttattg agggagatgt cgctgagaaa
      541 tccttgatgg aagagatcat gaaagctaat aaatttgatt atatctttca tttagcagca
      601 gttgctagtg tggcagattc agttgcccga ccagtagaaa cacatcgtgt gaattttgaa
      661 agtgtattaa tgattttaga attgatccgc aagtatcaac ctgatttaaa acggatcgtg
      721 tttagttctt cagcggctgt ttatggggat gaaccgactt taccaaaaaa agaagagtcc
      781 gtgattcgcc cattgacgcc ttatgcggtt gataaattcg ctgcagaaca atatgtgcta
      841 gattattgtc atttatatga tgtacccggt agcgcagttc gttttttcaa tgtctatggt
      901 ccaaatcaaa atccgaattc tccatacagt ggtgtgattt ctattctcgt agatcgctat
      961 aagaaacaaa tcgtaggaga agcaacttca tttacattat ttggtgatgg aagccagtct
     1021 agagactttg tttatataga tgatgtaatc caagcattac ttttagttgc gaatgaggaa
     1081 gctgcactgg ggaaacaatt caatgttgga acgggtaaag caaccacact tttggaattg
     1141 attcatgcaa tcaatgaaat tctcggtgta gaattgacac ttgaatacaa agaagagcgt
     1201 tcgggtgata tccatgactc tctagcagat attactaaga tccagtcaat tggctatcaa
     1261 ccgaaatatg gtattttaag tggaatgact aattatttga aaactgaaat caattaaaac
     1321 atactaagat aagattagag ggtaaattca ggctctttgt caataaggac tgatgagttt
     1381 tgtaaaatca aatctgggat agaagggctc tttgtcaaat gatgttggta aagagaaatc
     1441 ttagaataat ttggacagaa cagaaagaag aaaa
//
LOCUS       DACDRK010000171         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDRK010000171 DACDRK010000000
VERSION     DACDRK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.178"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWJ77_002787"
     CDS             complement(150..1337)
                     /locus_tag="IWJ77_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9454320.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DACDRK010000172         1413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDRK010000172 DACDRK010000000
VERSION     DACDRK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.179"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..281
                     /locus_tag="IWJ77_002788"
     CDS             <1..281
                     /locus_tag="IWJ77_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9454321.1"
                     /translation="KNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLR
                     ESVEEINEKSMLNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            473..871
                     /locus_tag="IWJ77_002789"
     CDS             473..871
                     /locus_tag="IWJ77_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9454322.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            1091..>1413
                     /locus_tag="IWJ77_002790"
     CDS             1091..>1413
                     /locus_tag="IWJ77_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9454323.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSK"
BASE COUNT          507 a          189 c          275 g          442 t
ORIGIN      
        1 ccaaaaataa aggagatttt gagaaagctg aaatatataa gaatcccctg attgaaatag
       61 ttaaaattcg tgagattacg aaaacagaaa atgagtataa aacaatatcg aattttgata
      121 gacgtgtttt aagggaatca gttgaggaaa ttaatgaaaa atcaatgctg aatattgatt
      181 ttgctaaagt tagaaaaggc agaaattaca attttgctgt ccagtttaat atttctagtg
      241 agtttgatga aaaaacttat agagaaaaaa aattaggtta atgcaaaata tatcttactc
      301 gtttgaagat gcttcagacg tatgctaaga tatatagtgc cttgggagca aggtattaat
      361 ctcctttaga ggttaattag cttgtgatcg cctatcaatt tttttggtag gcgattcttt
      421 tttttgaaac aaaaaagcaa aaattatttt ttaattagag aggtgcaatg cgatgaaata
      481 ctttttttgt gcagtattcg aatggaaagg aaatgaatat gcagttaaat tcccagattt
      541 tccagactta tatacacatg gtgaaacttt agaagatgct tttgagatgg cgcaagaagt
      601 attggaagga tacttggtgc ttttagagtt gcaagaaaaa gaaattccat cgcctacgcc
      661 tgttcaagag ttaaatgcaa gaatcaagcg atctgagaat gttaatttat ttgtcgtaaa
      721 agctaatacg aaagttgcta aagctcgaaa caagcaaaat tatgatcgaa aatcagtgac
      781 tgtggaacac tatttaaatg ttttagcggc tgaagagaat attaatctgt cgaaagcttt
      841 gaatgatttg ttgagagaaa agttgatttg atgtctctca gatgatcata ttaagcaaaa
      901 acagcttgtt ttaggacgta tggatttaac agaattaggc agaagtcgcc catcctctat
      961 aggtgggtga tgaatgccgt tcggtatgag ggttaccgtt ggtaactcga agaccgacaa
     1021 ctaaggcgta ttgccttttt attttgtccc atgttacaat cagtctatac aagaaatgag
     1081 ttgatataac atggaattag atacaaataa tcattcagta tttcttcttt attaccacct
     1141 tgttttagta acgaaatatc gtagacaagt gattgatgat gaaatatctg attatgccaa
     1201 aactactttt gaaagaattt cagaatcgta tcacattact ttagtggagt ggaatcatga
     1261 taaagaccac gttcatatta tgttcaaagc tcaacctaaa acagaattga caaaattcat
     1321 caatgcctat aaaagtgcca gctcaagatt gataaaacgt gactttccaa gagtcaaaca
     1381 atttctttgg aaagaaatgt tctggtctaa aag
//
LOCUS       DACDRK010000173         1399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDRK010000173 DACDRK010000000
VERSION     DACDRK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.180"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            53..1354
                     /locus_tag="IWJ77_002791"
     CDS             53..1354
                     /locus_tag="IWJ77_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9454324.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          454 a          283 c          257 g          405 t
ORIGIN      
        1 agaagctccg ttagaattga atcaccacaa cacaatcaaa ggagcgttct gtatgcctga
       61 tcttcatcat atcaaactac tacttggaat caaagataaa aacattttca tcacaaatgt
      121 ggaatcgaag agcattaaga atatcaagag tcttgttgtt tctgcgacgt taagtaaaga
      181 aattcgtcgc tgtcctttgt gtaaacagat gaatcatgaa ggcatgatcg ttaagaacgg
      241 aaagaaaaaa tcgctcattc aacttaataa atgtgcgaac caactgactt atcttgcttt
      301 agcgaaacaa cgctaccact gtcgtggctg ccacacctat tttacggcga atacctacat
      361 tgtcgataga aactgcttta tcgctaaaca agtgcgctat aaaattttag aagagctaac
      421 tgaaaaacaa gcaatgacga caattgctaa gcattgcggc gtttcttggt cgaccgtttc
      481 tcggacactc gcttctctga tccccatgac aaaagtaaaa agaaattggt taccgcgttg
      541 tttattaatg gatgaatttc gttcactgaa aaatcaagtc ggtccctact ccttttcttg
      601 tatggatggc gatactggaa aattactgga tattcttcct tcacggaaga aaaaggctct
      661 cgtttcttat tttatgcaat ttgaacgacg cgcacgattg aaggtaaagg tcatcgtgac
      721 ggacatgaat gtttcttatg cctctctgat aaaggaatgt tttccaaagg caaaactcgt
      781 tgtggatcgt tttcacattg tcaaacatct cattcgtaaa tttgaggata ttcgcgtacg
      841 aatcatgaaa agctttgatc gaaacgatcc aacacaagcc aaatattatc gacaattaaa
      901 ggcgttgtct cggcttctga tcaaacgaca ggacaagctt gtttatgaca aatggaccaa
      961 atggcgcaat tttggttggg cgtatctcac cgaaagtgaa gtggtggatc gtctgctttc
     1021 gatctctgac gaattacgaa tcgcctattt ttactatcaa gaaattcttc aagcgtttca
     1081 cgacaaagag gcggacactt tctttaaact tgtaagaacg atgcccaatt ccgttccaaa
     1141 agaactgcat tctataaaaa aagcttttat aaactatgaa tccggcattc gtttagccct
     1201 cgaattgcct tattcaaatg ccaaaataga aaatctgcat acccacatca aagcattgaa
     1261 acgagtcgct tatggctttc gaagctttcg gaaaatgaag accagaattt tcttactaaa
     1321 taatctgatt acctatgaat caaaaaacat ctaaagtaga aaaggaattc taccttagat
     1381 gttcttatct cactctatc
//
LOCUS       DACDRK010000174         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDRK010000174 DACDRK010000000
VERSION     DACDRK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.181"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWJ77_002792"
     CDS             191..445
                     /locus_tag="IWJ77_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9454325.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWJ77_002793"
     CDS             446..763
                     /locus_tag="IWJ77_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9454326.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DACDRK010000175         1357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDRK010000175 DACDRK010000000
VERSION     DACDRK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.182"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            262..1302
                     /locus_tag="IWJ77_002794"
     CDS             262..1302
                     /locus_tag="IWJ77_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9454327.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
BASE COUNT          553 a          188 c          221 g          395 t
ORIGIN      
        1 atattgtaat attacgatat cgtgatataa tgttattgta atatcgtgat atagttacca
       61 tgagcttaaa ccctgaaata gagcggtttg ctttaattta ttttttaagg taaactaaaa
      121 aaagataaaa aaagactact gctttaacaa agtttggcga cttatcagca gtagaaaaat
      181 atatagtgga tagatatatt tgtttaattc attgtaacat agttttgaac aaaacgctat
      241 cctacagaaa gaggattggc tatgaatgat tttaattatt acaaatcaaa agaaatttat
      301 cgtgaaaaat attatcaaat gcctaaggta ttttttacta acgaaaaata catggatctt
      361 tcaaatgatg caaaaattgc ttatatgcta cttaaagatc gatttgatta ttcagtcaaa
      421 aataactggg tagattctga tgataatata ttcttcatat tcacagttga agaattaatg
      481 aaattgttac aatgccgcga aggtaaagta tctaagatca aaaaagaatt agaaaaagca
      541 ggtcttctga aacagaagag aggaagagta aataaacgtg atggtaagat tgaatctatg
      601 cctaatttat tgtacttagg caagccagaa gttacgaatc aagacgtctt taaaatcatg
      661 gaagaggaag acaatactga ttccaccgta attgcgaaaa tcgcaaatac ggcaaaaccg
      721 cgaaaaatca atgattctac cgtaattgcg aaaatcgcaa atacggcaaa atccagtcat
      781 atcaaggact caaccgtaat tgcgaaaatc gcagacaatc tattctattc taattctata
      841 gacactaata gacacataat agacactgaa actgaccagt tacaaaatca agttttgcta
      901 gataacttcg tcgacatcat gcaagaggac agtataaata catttgtacc agaaaatgta
      961 ttaaacttga taaaaacatt ttcttcaagt tacagtgaag ctcaacaaac cgtccaaact
     1021 attcataatg caaagaaaaa agctgaagaa ttagagcaaa ctattgtcgt ttatgaagaa
     1081 ctggaatctt atggaattga tgctgacaaa ggattatata tgactttact gaaagcttat
     1141 caaaaacaaa aaactgaaaa agtaaacaat ttgcagaatc taattttcgt ttatgtaaaa
     1201 aattggtttg tcgaaaaagc tattgcggca aagcttgcaa acgaacaaag agaaacattt
     1261 aacgaattac caacagttag tacggaaaat tggttagagt agttgataaa aagcaattac
     1321 tataaaaatg atcaatttta aattagaact tttttat
//
LOCUS       DACDRK010000176         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDRK010000176 DACDRK010000000
VERSION     DACDRK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.183"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IWJ77_002795"
     rRNA            <1..>1355
                     /locus_tag="IWJ77_002795"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDRK010000177         1352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDRK010000177 DACDRK010000000
VERSION     DACDRK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.184"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>1352
                     /locus_tag="IWJ77_002796"
     CDS             82..>1352
                     /locus_tag="IWJ77_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9454328.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFL"
BASE COUNT          459 a          260 c          257 g          376 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg at
//
LOCUS       DACDRK010000178         1350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDRK010000178 DACDRK010000000
VERSION     DACDRK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.185"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1323)
                     /locus_tag="IWJ77_002797"
     CDS             complement(<1..1323)
                     /locus_tag="IWJ77_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9454329.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSN"
BASE COUNT          534 a          192 c          216 g          408 t
ORIGIN      
        1 attcgaatta tctaattcaa ttgtaagttt gttactcata aaatgagttg ttggatttat
       61 ttcctggtta cctagtaaat attgatcatt attaatcata tcaggatgat agattgactc
      121 tttgtttgca taatcagtat gattgtaatt ttcagctata gcaacagcgt cttcaggtgc
      181 tataagtgtt aatgaatctt tgttagcatg gatagaattg cttactcctg agagatgcaa
      241 tattagtgca agaaacgtaa agaccattat tttccatgaa ttatttttag tagaaaaaat
      301 ataatagatc actatactca tcgtataggc tataaataaa gaagaaaaac catttaaccg
      361 ccagacaaat tgtagactct taactggtgt atggttaaaa agttgccaag gaaataaatt
      421 ggttgaacaa aaaagaacaa atacaccaag aaagaaaata aataaatcat cagcagttaa
      481 tttttttata aaaataaaag caccaatcaa tccgagaaat aagaataatc caatagtata
      541 gttgttatag ttattattta aaatatgtgt cagcatatca ctaggagcca taccacttaa
      601 ttcttttccc agtgggactt tcaattcttg tgctctagat tgttgaataa aaggaattaa
      661 gaatcccgca cttaaaaaaa aggtgaggac ggtagctttt aaaagactta agagtctagc
      721 gattttttgg tcccaaaaat aaaaagaaag aaacaacgtt cctccaataa atacagaaac
      781 catagcgaca gaaagtaaat gagtatacac tactagcgtc atgccaatag acaaccagta
      841 ccatttttgg taatctctaa tataaatttc gtaacacccc attagtatca aaggcaaaaa
      901 tgttaaagcg acagcttctc ccaatgaagc acgatggaat atgtcaatgg cacgataggc
      961 ggaaaatgta taaataatcg aaaataacag cgaaatataa cgattgtttt ttatttgttt
     1021 catagaaaaa taagaaacga ccattgttaa aaaagtgata aaaaaatagt agatattata
     1081 acttaaaaca agatttccca tcattttata aaataaaaaa gctggataaa gagtaagcca
     1141 tggataaaaa atattcatca ttgttccatg atgatcgaag ttattgaagt ttactggaga
     1201 actccataca ttatctagtc ctatgattcg agataaatga aagtaagtat cctgattgac
     1261 actatgatag attcccttag acatgaatag agggatgacg taagcaaaac ttagaaataa
     1321 cataaaaatg atatctatta tttggttttt
//
LOCUS       DACDRK010000179         1341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDRK010000179 DACDRK010000000
VERSION     DACDRK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.187"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWJ77_002798"
     CDS             <1..1244
                     /locus_tag="IWJ77_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9454330.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          433 a          244 c          286 g          378 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgtacaggta ctgttctatg gggcgcttac
     1321 ttttcttctt caagaccaaa t
//
LOCUS       DACDRK010000180         1336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDRK010000180 DACDRK010000000
VERSION     DACDRK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.188"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..>1336
                     /locus_tag="IWJ77_002799"
     CDS             66..>1336
                     /locus_tag="IWJ77_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9454331.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFL"
BASE COUNT          450 a          253 c          257 g          376 t
ORIGIN      
        1 aacatatcgt tctctagtat gattaaatca cgacaaaata aacaactgag aggacgatat
       61 gctatatgaa tgattctatc aaaaaaatgc tgcgattaat agagaaagat ttgatgatta
      121 cagaggtctc ttacgagacc tttcagaaga aaaagacgtt gatcgtcgat gctgttttct
      181 cacctgctcc ccatacctgt agaaattgtg gttctactgt ggtagatgga aacgggaaag
      241 taattgtagt gaaaaatggg aagaaggaaa cgattgtccg ctttgaacaa tacaatcaca
      301 tgcctttggt tatgcgctta aaaaagcagc gctatacctg taaaaactgt cgaacccatt
      361 ggacggctca aagttatttt gttcaaccca gacattcaat cgcgaatcat gtcagatata
      421 aaattgcttc tttactgact gaaaaagtat ctttatcttt tattgcgaaa aattgtcagg
      481 tatctttgac caccgttatt cgtacgttga aagagtttaa aagctactta ccaaagcaat
      541 ctaagaggat tctccccaga gtattgatgg ttgatgaatt tcgttcgcac gcttccatag
      601 aagataaaat gagctttatt tgcgcagatg gcgaaacagg acaattaatt gatgtcttgc
      661 ctacgcgtaa attgcctcga ttaacaagct atttcttagg ctgcgctaat cctgaagaag
      721 taaaattttt agtcacagac atgaacgccg cctacttcca gcttaccaaa cgtattctgc
      781 caaatgcgaa agtagtgatt gatcggtttc atattatcaa acacatgaat caagccttta
      841 atgagttgcg tatccgtgaa atgaatgaac ttcgtaaagt aggacagaaa agtcaggcag
      901 aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc aaccattatg
      961 aatacaaaac atggaaaagt tttcgagcac caaaataccc atttcttacg gaagcgatga
     1021 tgattgatcg attgcttggc ttttctacgt ccctgaagga ggcgtatccc tattttcatg
     1081 aattagttga agcctttcga gacaaagacc ctgacttatt tttctcctta ttggcagaac
     1141 ttcccgaaac gttagatgac ggctttcggg aaaagctcca aaaccttctg acctatgaag
     1201 aaggcatcac caacgcgatg atctatcctt acaccaatgg aaaaatagaa gcgaagaata
     1261 cccatataaa gacaatgaaa cgagtatcct acggatttaa atcatttgag aacatgagaa
     1321 ttagaatctt tttgat
//
LOCUS       DACDRK010000181         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDRK010000181 DACDRK010000000
VERSION     DACDRK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.189"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            278..820
                     /locus_tag="IWJ77_002800"
     CDS             278..820
                     /locus_tag="IWJ77_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454332.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            910..>1324
                     /locus_tag="IWJ77_002801"
     CDS             910..>1324
                     /locus_tag="IWJ77_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9454333.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DACDRK010000182         1324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDRK010000182 DACDRK010000000
VERSION     DACDRK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.190"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(51..1190)
                     /locus_tag="IWJ77_002802"
     CDS             complement(51..1190)
                     /locus_tag="IWJ77_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9454334.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          344 a          287 c          244 g          449 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtttat taaaaaaatc ttacgctaag
       61 taggataaaa taattccttg atatttttcg atcatatcca aataattatc gatttcaaca
      121 tactcatcta cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag
      181 gaatggttag ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt
      241 aatggctggc tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg
      301 acaggtattt tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat
      361 tcattgacga ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat
      421 cgaatgttcc cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg
      481 atcaatgtaa cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg
      541 tgattcattt cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa
      601 ccttggtctg gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt
      661 gatcccatgt gcgtatacat caaactgtaa ttggttggct ctccgatgat caaggcatct
      721 aagtcatcta catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct
      781 ccgacagtgg ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg
      841 atcatggcaa tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac
      901 aaacggttcc catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca
      961 gccgccacga catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg
     1021 taacgagcaa tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca
     1081 tgctttgcca aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat
     1141 ttaattcgga taatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct
     1201 cccttgccat ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca
     1261 aagtaaaaat gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt
     1321 ttat
//
LOCUS       DACDRK010000183         1318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDRK010000183 DACDRK010000000
VERSION     DACDRK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.191"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            188..391
                     /locus_tag="IWJ77_002803"
     CDS             188..391
                     /locus_tag="IWJ77_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454335.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            602..1186
                     /locus_tag="IWJ77_002804"
     CDS             602..1186
                     /locus_tag="IWJ77_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9454336.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          465 a          182 c          252 g          419 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca ctttcttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag
      901 ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt
      961 gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg
     1021 tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg
     1081 tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta
     1141 tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa
     1201 tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag
     1261 tgtctactga taacttattc tcaaatgttg taaggatttt attcatttgt taatatta
//
LOCUS       DACDRK010000184         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDRK010000184 DACDRK010000000
VERSION     DACDRK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.192"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWJ77_002805"
     CDS             <1..848
                     /locus_tag="IWJ77_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9454337.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DACDRK010000185         1308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDRK010000185 DACDRK010000000
VERSION     DACDRK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.193"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            26..>1308
                     /locus_tag="IWJ77_002806"
     CDS             26..>1308
                     /locus_tag="IWJ77_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9454338.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          409 a          285 c          261 g          353 t
ORIGIN      
        1 aaaccattca aaggagtgtt tcaagatgtc ctattcagag tttaccaaag agttactaga
       61 tattctagac ctaaatctta cctttcatga agatgccttt cgcaaagagc gcattaatga
      121 cgaaacatgt tttgtttttg atggcacatt gacctatcaa ccagaggaat gcttccattg
      181 ccactatcaa aataagcaaa cgatcattaa atggggctgg aaaaaagtat ccatcttatt
      241 gaatgatgtc agtaactaca agaccattgt gcggattaac aaacagcgct tcaaatgtaa
      301 acactgtgga aagacttttt tagcagaaga ttcagttagc gatcgtcgct gctccattgc
      361 ccgcagagtc aaacaagcca ttcttgagct attaagtgag cctatttcta tgtctttgat
      421 tgcacgtatg aagcacattt ctccgaccac tgtcattcga atacttcgca gccttcggcc
      481 taaaaccgtt tctttgaacc agccgttacc agaagtggtg tgtttcgatg aattcaaatc
      541 ggtgaaaaac gtctcgggag ccatgagctt tgtcatgatg gacggcaaga cccatcaact
      601 gattgatatt gtggaaaatc ggcaactcaa tcctttacgc gactatttct tgcgctaccc
      661 tagaaaagtc cgtgaacaag tacgtctagt ggtttcagat ttctatacac cctatcgcac
      721 attggtgaaa gagtgttttc cgaatgcccg aatcgtcgcc gatcgctttc atattagcca
      781 acacatcggt cgggccttta ccaaccaccg aattcaagta atgaagacct ttaaaaaagg
      841 ggatcgtcgg tcgaagcact taaagaaata ctggagacta cttcaaaaga atgcgtggga
      901 attgaatggt cagcatcgct actggcgtcc gtcatttcga gatcatctga cagaagcgga
      961 gatagtcgat cggctattat attatgatga ctccttaaag cgaggctacg aagtctacca
     1021 ggtttttctt tccgctatca gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga
     1081 agactacaag gagttgccag agcactatca accagtgttc acgaccttca agaaatatcg
     1141 aacagaaatc aaacgagcgt tgcgagttcc ctattccaac ggccctatag aatgtttgaa
     1201 caaccatatc aaagtactaa aacgaattgc ctatggcttt agaaactttc aaaactaccg
     1261 agaacgaatc tttttatatc gaggaaaata tttcaagaaa actaagaa
//
LOCUS       DACDRK010000186         1286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDRK010000186 DACDRK010000000
VERSION     DACDRK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.194"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            36..>1286
                     /locus_tag="IWJ77_002807"
     CDS             36..>1286
                     /locus_tag="IWJ77_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9454339.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          398 a          256 c          250 g          382 t
ORIGIN      
        1 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
       61 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
      121 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
      181 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
      241 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
      301 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
      361 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      421 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      481 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      541 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa
      601 gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt
      661 ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc
      721 cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc
      781 acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac
      841 gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga
      901 aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt
      961 ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact
     1021 tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt
     1081 cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca
     1141 ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt
     1201 caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg
     1261 gctttcgcaa cttttacaat ttcaaa
//
LOCUS       DACDRK010000187         1258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDRK010000187 DACDRK010000000
VERSION     DACDRK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.195"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            73..690
                     /locus_tag="IWJ77_002808"
     CDS             73..690
                     /locus_tag="IWJ77_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454340.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          463 a          169 c          204 g          422 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg ttatttaata
      661 agatgctgcg gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt
      721 tcatagcaat ggagaaaata cattagcaat atataaaaga aggtatcaag agtcacagtg
      781 aatttattcc gaaaagtgaa aagagcaaat ataaaattta atgaaaatat agaactaaat
      841 aggaaaacgt agacgtaatt ttctacgttt tccttttcat atatattaaa tattacggaa
      901 aattatataa actaatttag tgttttgttt gtaattccta attttgctcc acttttagat
      961 aatttttagg tctatttaac tcatcagaca ttctatgctc atttttctcc aaatttgttt
     1021 caactctttc gtacttaatt ttgcgtattt gaaaattctt cttttagagt acttttatta
     1081 cgatactagt atgatatata tctatagcaa acagttaaaa tcttcctaaa ataagcgaat
     1141 agcattccta aaacctataa aatttttaga tgaagcgaca agtggacaat taataaacta
     1201 cttgaacatg aaattatgga tcacctttca aatttagaaa atggaattct tatactta
//
LOCUS       DACDRK010000188         1252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDRK010000188 DACDRK010000000
VERSION     DACDRK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.196"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(74..1078)
                     /locus_tag="IWJ77_002809"
     CDS             complement(74..1078)
                     /locus_tag="IWJ77_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9454341.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT          349 a          234 c          178 g          491 t
ORIGIN      
        1 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
       61 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
      121 ttcttccatc tgtataaaca cggaattgtt ttccaccagc cattgttgta tttccattat
      181 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
      241 tatattcatt tgtgctaatt aaattagtat ttgatgcatt tttatcagga gaatcataca
      301 accataaagc tttattattt agtacagttt ctttagcagc ttcccaactt gaaattgctg
      361 agctacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
      421 gagtttgagg agtttgtcca ttcacaatat caatgaaacc ttgttttaac tgttggttct
      481 gagtttcatt gaagtacatg tagtttttat cattgccttg attttgttgc gttaaataaa
      541 ctttaggttg tccattttga aagccaaaga aatatacatg ctgccctaaa tattctccag
      601 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
      661 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatttcca tcaataactg
      721 cacttggtaa aggcgtacca tacagactta cattgttctg aggattgtag cttttatatt
      781 cttgacctaa tgtctttccc cattgtggga caaaaattgc tagttgagta gatttctcac
      841 tatcccacaa agtattttta acctcttcag aagaagattc aatgattgaa ctttcacttg
      901 tacttgcttc cacagattgt ttcatttctg ttgtagtttg ttcagttgat tgcgttttac
      961 tttttttagc ctcaattgtt gtatcagttt ctacagtctc tttagtcctc gtattctcag
     1021 atgtacaacc agtgaataaa atccccattc caattagtaa taacaaccat tttttcattt
     1081 tatttccccc tttcaatttc tccctcgcgt acttatttta tcagtcaatt aatctcaatg
     1141 gtgttcattt agttaatcct ttcccaagaa tgtcgatagc cagcatgaca acgatctctt
     1201 ccttgagggc tttcagatgg attaaaacta gtagaaattt cccttccaca tc
//
LOCUS       DACDRK010000189         1234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDRK010000189 DACDRK010000000
VERSION     DACDRK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.197"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1234
                     /locus_tag="IWJ77_002810"
     CDS             <1..>1234
                     /locus_tag="IWJ77_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9454342.1"
                     /translation="KSSFWYTIGNFFIKGISFITIPIFVNITTQAEYGLINNFTSFVS
                     IFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLLIGNIYFLFSNAFF
                     DMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTFLNIAISVALMQTL
                     YFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALKISLPLIPHSLAN
                     IVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEK
                     KVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSL
                     PVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFHYN
                     IAKKITNKQLFDTKKICII"
BASE COUNT          387 a          155 c          195 g          497 t
ORIGIN      
        1 taagtccagt ttctggtata ctatagggaa tttttttatt aagggaattt cattcataac
       61 tattcctatt tttgtaaata ttactactca agcagaatat ggtttgatta ataatttcac
      121 ttcatttgta tcaatttttt cgattcttgt tgggctaagc ctaaattcgg caattaataa
      181 tgcaagtttt gactataaag atgacataaa tgaatttatg tcatcggtac tttctctagc
      241 tacactttcg ttaattgtgt tcttattaat agggaacata tattttttgt tttcaaatgc
      301 tttttttgat atgaaccaac ttgtatttaa tttgatgtta ttacaaagtt ttgctagttt
      361 tgtaattagt tatatccagt cttattttac gattaacttt aaccatttta aatatttgat
      421 tttatctttt atatctactt ttttgaatat tgcaatctct gtagctttga tgcagacatt
      481 atattttgat gataaatatg ttggcagagt agtcggcgga acactgtcat ttgtgttgat
      541 aggtttagta atttatttaa gaataatgat aaagggtgct catttttttg ataaaaaaca
      601 gtggagatac gctttaaaaa tctctttgcc gctaatccca cattccttag caaatatagt
      661 tttagcacaa ttcgatagaa ttatgattaa ttcttattta ggtcctgctg cggggggaat
      721 atattcctac attagtaaca taggtattat tctaagcgta atatggatgt ctactaataa
      781 cgcctgggtt ccatggttct atggtgagat gagtaaaaat aatgaaaaaa aagtgaagaa
      841 agttagtaac tattatttag tagtctttac tttgattacg atgattgtaa tggtagtttc
      901 gattgattta gcaaaattta tggctccgaa agactattat tctgggttat ctttagtggt
      961 tccgataacc ttaggctatt tctttcagtt tttatatagt ttaccagtta atgctgaatt
     1021 ttatgagaaa aaaacatcgt ttattgcgat tggtacagta gcaagtgcta tagttaatat
     1081 actattaaac atgattttca tacctaggtt aggaataatt gctgctggat acacaaccgt
     1141 atttacgtat tttttacttt ttatttttca ttataatatc gctaaaaaaa ttacgaataa
     1201 acaattattt gacacaaaaa aaatatgtat cata
//
LOCUS       DACDRK010000190         1216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDRK010000190 DACDRK010000000
VERSION     DACDRK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.198"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..305)
                     /locus_tag="IWJ77_002811"
     CDS             complement(<1..305)
                     /locus_tag="IWJ77_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454343.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEF"
     gene            complement(322..>1216)
                     /locus_tag="IWJ77_002812"
     CDS             complement(322..>1216)
                     /locus_tag="IWJ77_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9454344.1"
                     /translation="AMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHE
                     PLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNL
                     SNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSL
                     PEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSE
                     TKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKK
                     YAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          422 a          207 c          138 g          449 t
ORIGIN      
        1 ataaattcat catgtatcaa aaagaataaa taacttgata ttttagcatc tagcttaatc
       61 agattaaata atgtttgttt aaaattgcta ccattaatat gactgataga ttctctagct
      121 tcttcacata aactttttac tttattaatt actactttgt catattcatt taacacacta
      181 ttattaccat tacttagacg ctcagattct acgaaacaac tcaaatcaga aatttcccaa
      241 aaaaattttt tgttaaaatc caatttcctc aactcctcca aactacgtaa atgatatagt
      301 ctcataataa aataaaacct ctcagctaat gttgttgaaa ttgtccacta aacctaattg
      361 atattgctta ggagatacac ctgcgtactt tttgaatact ctcgaaaagt gtgaagaatc
      421 tgaaaatcct aaacctaaag aaacttcact gatagtatgg tttgatttca acattatttt
      481 agccgtagaa atttttcgct tagagatata attataaatc gtaatatgca tttcattctt
      541 aaagctttct cttaatttag tttcacttac agaaaaataa ttagctattt cctttacagt
      601 aattctttta tagatattta agccgatgaa ttgaacgaca gaagaaatta agggagaaag
      661 atgctctatc ttagtttttc cgatttcctt agtataataa acaatcccag aatccctcac
      721 ttttaagacc tcaggtaacg atacagctaa ttcgttttct tgtatcaaag catctctact
      781 ttgatacgct tctattatat ccattcctaa tgttatagac gtttgtgcta acttttcaaa
      841 aacaatgata gaataatttt tttcagaccg aatcgtatct ccactaacac ttggaattat
      901 tccattactc agattaaaaa ccatgttttc taaatttcga atatctccat ttttaacata
      961 ttccagtatt ttattttcat aaagaaacag atatatttct ggatcgtaat tttgtgataa
     1021 cagattatct attttgattt gctcgatagt tttacttaga ttttctgtat attcaattaa
     1081 cggttcatgg aatagatctt ctatttttcc agtaaaaaaa taatgaacta aaatcagaat
     1141 atctcgaatg tctcctaaag aatacaaagt caaattttgc aatgaattat agagtcgttc
     1201 tctatcagcc attgca
//
LOCUS       DACDRK010000191         1192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDRK010000191 DACDRK010000000
VERSION     DACDRK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.200"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWJ77_002813"
     CDS             <1..1076
                     /locus_tag="IWJ77_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9454345.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DACDRK010000192         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDRK010000192 DACDRK010000000
VERSION     DACDRK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.201"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWJ77_002814"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWJ77_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9454346.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACDRK010000193         1132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDRK010000193 DACDRK010000000
VERSION     DACDRK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.202"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..1130
                     /locus_tag="IWJ77_002815"
                     /pseudo
     CDS             1..1130
                     /locus_tag="IWJ77_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
BASE COUNT          487 a          149 c          190 g          306 t
ORIGIN      
        1 atggaaacaa atggtctttc tatccaagaa actgctaaaa aatttaatat cggttcaaat
       61 agaactctaa gtaaatggat agagcaatat gaagaaggcg gtgcttcttc acttgagagc
      121 caaaaaaggg gcagaaaaat tagtatgaat tccaagctaa acattcctaa aaaacttaaa
      181 gatgagtctc ttgaagaaga agttattcgt ttaagagcag agaacgcata tttaaaaaag
      241 ttagaaacct tgattcaaga acaagatttg agcaagaaga aatcaaggtt aaaacaatcc
      301 ttcagctcaa aaaagaattt aaattgaatc tattactttc aattgcacaa ttagcgaagt
      361 caacttatta ttactgggta aaaaaattag ataagccaga taaatatagc aagataaagc
      421 aagaaattac agcgattgta aaagaatcaa gaaactctta tggttatcgt agagtcactt
      481 tagcgttaaa gatgaaagga tatacaatca atcataaaac agttagaaaa ttaatgtccc
      541 aaatgggact tacctgtcaa atccgaataa agcgatataa atcttataaa ggaacagtag
      601 gaaaaattgc caagaatgtg ttaaaacgaa atttttcagt agatacaccc aataaaaaat
      661 gggtgacaga cgtcactgaa ttcaagataa aaggaagaaa aatctatcta tctcctatac
      721 ttgatttatt taacggagaa ataattagtt atagtatatc aactagtcca acatataaac
      781 tgattgaaga aatgcttcaa caagcaatta aaaaaaaggg aactgagggt tcactgattc
      841 tacattcaga tcaaggatgg cagtatcaga tgccacaata tcaaaaaaaa ttaaaagaga
      901 ataacattat tcaaagcatg tctagaaaag gaaattgctt agataattct gtaatagaaa
      961 atttttttgg tgtgcttaaa tcagagtttt tttatcgaga aaaattccga tcaattgaga
     1021 tatttcaaag taaattaaat gagtatatta ggtggtataa taacaaaagg ataaaactaa
     1081 agttaaatgg actatctcca gtagaatatc ggaaacagtc cattaaatag tt
//
LOCUS       DACDRK010000194         1117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDRK010000194 DACDRK010000000
VERSION     DACDRK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.203"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..900)
                     /gene="vanY"
                     /locus_tag="IWJ77_002816"
     CDS             complement(<1..900)
                     /gene="vanY"
                     /locus_tag="IWJ77_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9454347.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTN"
BASE COUNT          329 a          228 c          159 g          401 t
ORIGIN      
        1 attagtatgt gttgatccgg gaaacactgt cacaattaca ccgtctatat tgtttcctga
       61 tatctcataa cgaagattag tcggcacatg aatggtggta tttttagtaa caggataata
      121 aaagatctca tatttttccc catttacact aacagaaatg gttttttctt cttttaggta
      181 atccatatat tcctcgagaa cgaaattctt ttctttcata atcgcactat gtggtaaacc
      241 aacatagcga atatgccatg gttcatattg aattcctgtt aactctgttt tgtcctctgg
      301 ataacgtaaa atgaacccgt atttccaagc attttcttct atccactttc cttcaggggc
      361 tcgttccatt ttcgtcaagc ttgatcctac atctagtgat aaacctgaat tatgctcact
      421 ataacctgct ggtaaggcat actcagcccc catttcttgg taaagcacac tttgctcatc
      481 aaagtctcga tagccactat taataataaa atgactaacg ccacccttta cagcatcatt
      541 gaccatctct gaaaattttt gtgctatttc ttttgacata taaatattac tatcaagcaa
      601 cccgtatcca tttattaatt cgtcatgttt agataaattc acgatatctg acttcacact
      661 ttcttggcga acaggatatt tactattgat taatagcaga tttccttgat aaacctgttc
      721 ttctgtaatt gttttctctt gggtattttc agaagtccca ctattttcta aatgttcttt
      781 gggattttga tcataatttt gaaattcgac tttttcctga gaaaacagtg cttcattaac
      841 gtagtcataa cctaagtata ttaagaataa caataacaat aaaaaaaaca acttcttcat
      901 tttcagtctc ctccttaact atatagttgg aagtatgaaa tatttctaga atcttcccct
      961 taaacggcac aatctcgttt gagattgtgc cgttttgccc cgtaaattac agtcaattat
     1021 ttcgccctac tggatttact attatcacca atgtagatga attttcataa gtgataccca
     1081 tgctgaattt aacgaggctg aactgcctgt tgacaat
//
LOCUS       DACDRK010000195         1084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDRK010000195 DACDRK010000000
VERSION     DACDRK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.204"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IWJ77_002817"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IWJ77_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IWJ77_002818"
     CDS             complement(545..739)
                     /locus_tag="IWJ77_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454348.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          376 a          186 c          157 g          365 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacg
//
LOCUS       DACDRK010000196         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDRK010000196 DACDRK010000000
VERSION     DACDRK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.205"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>1073
                     /locus_tag="IWJ77_002819"
     rRNA            2..>1073
                     /locus_tag="IWJ77_002819"
                     /product="23S ribosomal RNA"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DACDRK010000197         1063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDRK010000197 DACDRK010000000
VERSION     DACDRK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.206"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..994)
                     /locus_tag="IWJ77_002820"
     CDS             complement(38..994)
                     /locus_tag="IWJ77_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454349.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt caa
//
LOCUS       DACDRK010000198         1045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDRK010000198 DACDRK010000000
VERSION     DACDRK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.207"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWJ77_002821"
     CDS             6..980
                     /locus_tag="IWJ77_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9454350.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DACDRK010000199         1019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDRK010000199 DACDRK010000000
VERSION     DACDRK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.208"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..282)
                     /locus_tag="IWJ77_002822"
     CDS             complement(<1..282)
                     /locus_tag="IWJ77_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454351.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTK"
     gene            complement(384..737)
                     /gene="tnpB"
                     /locus_tag="IWJ77_002823"
     CDS             complement(384..737)
                     /gene="tnpB"
                     /locus_tag="IWJ77_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9454352.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(727..921)
                     /locus_tag="IWJ77_002824"
     CDS             complement(727..921)
                     /locus_tag="IWJ77_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454353.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          289 a          204 c          181 g          345 t
ORIGIN      
        1 tttcgttcct acaactcgtt gatgtccttt tactggaata atggtggcag ctgcttctgg
       61 aacacttaca gaaatttctt catcactaaa aagattcaat tgcccattcg tttcctcaaa
      121 gagagtctct ttagagcgtc caaataattt gttggttaaa taattgacct tttcattcaa
      181 tagttggatt tcagttgtaa gattctgaat ggtttgttgt tgtgcttgat tggttttatg
      241 ggcttcttga agcaactgaa gcaacgtatc tgtagtttcc aaaatttttc accaccttga
      301 atcgaactga ttttgttaca gaataccaca agaaacgcac aagaaatagg ggttcaacac
      361 attttttgtg ttaacccccc agcttaaaaa actccttttt ttcctggcaa aatagctcga
      421 ggttgatcga tacttaatcc ttctaacaac catcgaacct gttggttcgt taaggttcga
      481 acttcgtctt tcgtccgagg ccattttaac ttgccgtttt ctatccgctt ataacagaga
      541 agaaaaccat caccgtccca atagagaagt ttgaaacggt cttgacggtt cccgcaaaat
      601 aaaaaaacgg catcttcgta aagctcgagt tgatactctt gttgaataag actggcaaga
      661 ccatcaattc cttttcgcaa atctgttttt ccgcacacaa tataaatatg tttggtttga
      721 agtaaatcat aaagcattgg acaacagttc tttcaaaata gcttgaagaa tataattgtt
      781 aataccgtta tagatcaaaa cttcagaacc attctctctt ttgatgcgca tcgctaactt
      841 ggcagccgcg ggtttacctt tttctggtgc actcttttcg tttcctaatt ggacaggaac
      901 aatctcattt aatttataca tagaagacct ccagacttct tgatagctca agtatatctt
      961 gaggtgcatt tgttagtaag gtactgttct atggggcgct tacgttaaaa atgttttgc
//
LOCUS       DACDRK010000200          995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDRK010000200 DACDRK010000000
VERSION     DACDRK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.209"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..913)
                     /locus_tag="IWJ77_002825"
     CDS             complement(26..913)
                     /locus_tag="IWJ77_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9454354.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DACDRK010000201          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDRK010000201 DACDRK010000000
VERSION     DACDRK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.210"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IWJ77_002826"
     rRNA            complement(<1..>994)
                     /locus_tag="IWJ77_002826"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDRK010000202          982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDRK010000202 DACDRK010000000
VERSION     DACDRK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.211"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..461)
                     /locus_tag="IWJ77_002827"
     CDS             complement(<1..461)
                     /locus_tag="IWJ77_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9454355.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(745..966)
                     /locus_tag="IWJ77_002828"
     CDS             complement(745..966)
                     /locus_tag="IWJ77_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9454356.1"
                     /translation="MKEKFLNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRV
                     AYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          313 a          188 c          131 g          350 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa agaagccgtc
      721 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
      781 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
      841 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
      901 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
      961 tttcaatggc ttctctttta gc
//
LOCUS       DACDRK010000203          967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDRK010000203 DACDRK010000000
VERSION     DACDRK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.212"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..185
                     /locus_tag="IWJ77_002829"
     CDS             <1..185
                     /locus_tag="IWJ77_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9454357.1"
                     /translation="NSEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFAS
                     SLMLIKKPKEILSKIS"
     gene            195..851
                     /locus_tag="IWJ77_002830"
     CDS             195..851
                     /locus_tag="IWJ77_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9454358.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT          400 a           95 c          158 g          314 t
ORIGIN      
        1 ttaactcaga ggattcaatg atttccggtg gaagtttaat aaataatagt agttttatgt
       61 caaatataac aacacttgga caaagttatt taatattaat aagtattatt gttttatcag
      121 ttgtatttgc ttcttcatta atgttaatca agaagcctaa agaaatctta tcaaaaataa
      181 gttaggagaa aataatggat atattagaaa taaagaatgt agcatatagt tatgcaaatt
      241 ctaaagaaaa agttttgtca ggagtaaatc aaaaatttga acttgggaag ttttatgcga
      301 tagtaggaaa gtcaggaaca ggaaaatcta cacttctttc cttacttgct ggacttgata
      361 aacctcaaac aggaaaaata ttgtttaaga atgaagatat acaaaataaa ggatatagta
      421 atcatagaaa aaataatata tctttagtat ttcaaaacta taatttaata gattatttat
      481 caccaattga aaatattaga ttagtaaata aatcagcaga tgaaagtatc ttgtttgaac
      541 taggtttaga taaaaaacaa ataaaaagaa atgttatgaa attatctggt ggacagcaac
      601 aaagagtagc tattgctaga gcattggtat cagatgctcc aataatacta gctgatgagc
      661 ctactggtaa tctagacagt gttactgctg gagaaataat taatatatta aagacattag
      721 ctaaagatag aaataaatgt gtaatagttg taacacatag taaggaagta gcagattctg
      781 cggatatcat tttagaacta agtggtaaaa agttgaaaaa agtaaataaa atgaatttgg
      841 aggttgaata atgataaaaa atgcatttgc ttatgtaact agaaaaagtt taaaatcatt
      901 aattatcata ttagttattt tatctatgtc aactttaagt ctcattagtt tatctattaa
      961 agatgct
//
LOCUS       DACDRK010000204          958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDRK010000204 DACDRK010000000
VERSION     DACDRK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.213"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            85..354
                     /locus_tag="IWJ77_002831"
     CDS             85..354
                     /locus_tag="IWJ77_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9454359.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            347..604
                     /locus_tag="IWJ77_002832"
     CDS             347..604
                     /locus_tag="IWJ77_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9454360.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          366 a          102 c          178 g          312 t
ORIGIN      
        1 taagaagtta gaatattaat catttgctta attgtacaat ataatgtaca attgttttat
       61 agaaataaat aaggggtgaa aggaatggaa gcagtagctt attcaaattt ccgccaaaat
      121 ttacgtagtt atatgaaaca agttaatgag gatgctgaaa cacttattgt aacaagtaaa
      181 gatgtagaag atacagttgt tgtattatca aaaagagatt atgattctat gcaagaaacg
      241 ttgagaacac tttctaataa ttacgtcatg gaaaaaattc gtcgaggaga tgaacaattc
      301 tccaaaggtg catttaaaac acatgactta atcgaggttg aatctgatga ttaaggcttg
      361 gtctgatgat gcttgggatg attatcttta ttggcatgag caaggaaaca aaagcaatat
      421 aaaaaagatt aacaagttaa taaaagatat cgatcgttcc ccctttgctg gattaggaaa
      481 acctgagcca ttaaagcatg atttatctgg aaaatggtcc agaagaatta cagatgaaca
      541 tagactgata tatagagttg aaaatgaaac gatatttatt tattctgcaa aagatcacta
      601 ttaaccaatc ggaagtaagg aaagggtcag aaacttaaaa gtttttgatc cttattttat
      661 ttaccctagt catttaaaaa gctaatatag cttagtgttg attgttatta atgaatgtgt
      721 ttgttacgcg tattacggat ataaggttag taaaatcatt tctaaagttg aggaaaagta
      781 aatataaatg gcttaaattt caacaatttg aagttgaata gatatgttat aatactattg
      841 tagtgtggga tgttagttac taaaggatga cgcttatata tatgactgaa tagaataagc
      901 aataggttta ataatctatt ttaaattttt tgtactagtt ttagttaatt agcaaaaa
//
LOCUS       DACDRK010000205          940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDRK010000205 DACDRK010000000
VERSION     DACDRK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.214"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..365
                     /locus_tag="IWJ77_002833"
     CDS             <1..365
                     /locus_tag="IWJ77_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9454361.1"
                     /translation="VLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGD
                     AVDQEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELF
                     ISILKELEEHENWYAEVE"
     gene            366..914
                     /locus_tag="IWJ77_002834"
     CDS             366..914
                     /locus_tag="IWJ77_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454362.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT          378 a          132 c          200 g          230 t
ORIGIN      
        1 tcgtcttaca gacccaagta aaatcgatga gaaatgcgat gcaatatctt gagtatatgg
       61 acttttttgt gaaagaattt gatacaaatt tatatgttgc tggtgttatt cctttcatgt
      121 tggaaagcgg tgacgcagta gatcaggaaa tgtataaaca agcccaagag atttatgggg
      181 aacatttgat taaaaatgtt gtactcaaaa atgcgcgctt aaaacgatac gacggatcag
      241 gtattacgat ggagaagaca aagaaaggtc tcttaaaaca atgggatcgc aaaagccacg
      301 aattatttat cagtattttg aaagaattgg aagaacacga gaattggtat gcggaggtgg
      361 agtaattggc tgacatcaaa ggacttgata tttttgggaa caacaaagga ctcgccaaaa
      421 taaaaaaaga agcagaagca aagaaaaagc agcaagcaat tacaccatct gaaaataagc
      481 aattagaagt gcaccaagtt caagaaataa aaacttcctc caatgttgaa agacagagta
      541 gaggaagaac aaaaaaagtt ggcgcaccaa tcagaaagtt tgataaggtt tattccacca
      601 agcatccttt aaaactgtcg actttgttaa atgcgacttc cagagtttta gtagaaaagt
      661 atgagactaa tatgacaaga gatgaattgc ttagaaaagc tttagatgag tatattaaac
      721 agaatcttac gcaagaagat aagcaagatt tgtttaatga cgtggtgaaa gaactagact
      781 tgttcagaga aaaatatcca attgttccag aattagatga tcgtggagaa ataataagaa
      841 ctaccagtca gataaaacaa gataccgaag actcactgcg caaagggtgg ggaatgaata
      901 ataaagttag ataataatca aagaagaact aagtaactac
//
LOCUS       DACDRK010000206          933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDRK010000206 DACDRK010000000
VERSION     DACDRK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.215"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(342..>933)
                     /locus_tag="IWJ77_002835"
     CDS             complement(342..>933)
                     /locus_tag="IWJ77_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9454363.1"
                     /translation="KEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGG
                     ALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQ
                     EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNG
                     YVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          253 a          220 c          180 g          280 t
ORIGIN      
        1 cttctatcat tacaagctcg tttggtgtaa gatgggtata agtcatttat gttcactctc
       61 cttgtatgct ttagcgggta ttacgatttg agtgtaacat aaatggcttt tttatttgtc
      121 tcgcttaatt atacaaacgg cgtgtaaaaa actattgcac aatacaagtg gcctacggct
      181 atcgaaattt ccataacttt cgagcaagaa tttatctcat tcaaggcttg atcttccaag
      241 taaaacaaaa accagttaag cactctgcct aactggttat gactaaattt tcagttgtat
      301 ctttctctac caacactaaa tgtcgaagag ccttttttcg tttatgaatg taagaccgct
      361 tctcgagcca atgccaaaca accgagcgtt ccagcgttgt cttctaatgc tggggtcacg
      421 atatatttgc ttaactcagg tgtttcgaca tacccattga ccaattttgc aaatgcgtcg
      481 tggacgtttt gcagcatatg gcgttgtttc atcacaccac cgccaaaaat gatcacatct
      541 ggagaaaaca tcaaggttgt attgtaggca cattgagcaa tataggaggc ttcgatttcc
      601 caataaggat catcttctgc aacctcttgt ccttttttcc ctaagcgttt ttcgatggcg
      661 ggtccagcgg ccatcccttc taaacagttg ccatgaaatg gacagctgcc agcaaagtca
      721 tcttttggat gagggacgac gagcatatgc cccatttctg gatgactaaa cccttcgata
      781 aagcgccctt cttgtaaggc accgccgcca acacccgtac cgatcgtata gtaaacgaca
      841 ctgctcaatc ctttgccact gccgaagaca tactcaccat aggcagccgc attgacatct
      901 gttgtccaag aaatcgggat cggaaattct ttt
//
LOCUS       DACDRK010000207          908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDRK010000207 DACDRK010000000
VERSION     DACDRK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.216"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            275..908
                     /locus_tag="IWJ77_002836"
                     /pseudo
     CDS             275..908
                     /locus_tag="IWJ77_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          303 a          172 c          170 g          263 t
ORIGIN      
        1 atgtaatcat caggaaaaac aatcttaaac gaattactat tcatggtctt agacacacac
       61 atgctagctt gcttttagca agctagtgca aatattaaag atgtgcaaga gcgtttaggc
      121 cactcttcta ttcaaatcat tatggatcta tatattcata ttacagacaa aagaaaagaa
      181 gaaaccgtag cgcaattcgc aaaatatagg ctctataatt tatgggactg atgaatcaaa
      241 attgattctt cagtcccatt gttattttag gtatatgatt cgtacattga aagagttgaa
      301 aaactatctt ccgaagccgt caaaaaggac cctgccaaaa gtattgatgg tggatgaatt
      361 tcgttcgcac gcttcaattg tggataaaat gagctttatt tgtgcggatg gcgagactgg
      421 aaaactcatt gatattttgc ctacacgaaa attgtctcga ttaacacact attttagtcg
      481 ctgtgcaaat cctgaagcgg tggaatattt agtgacagat atgaacgccg cctacttcca
      541 actgacaaaa cgtgtcttac caaatgcgaa agtcgtcatt gattgttttc atatcatcaa
      601 acatttgaat caggcgttca atgattttcg tgtacaagaa atgaaaagat tacaccagtt
      661 agggcaacga cgggaagccg agaaagtgaa aaagaactgg cgctttcttc taaaaaaccg
      721 tcaaaaaatc aatcactatg attacaaaac atggcgcagt tttcgtgcac ctaaataccc
      781 gctactcact gaagcaatga tgattgatcg attgctggca ttttcaacgc ctttaaagga
      841 cgcctatact tttttccatg agctgaccga agcctttaga gacaaggaat cagacttatt
      901 ttttacct
//
LOCUS       DACDRK010000208          898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDRK010000208 DACDRK010000000
VERSION     DACDRK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.217"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            69..>898
                     /locus_tag="IWJ77_002837"
     CDS             69..>898
                     /locus_tag="IWJ77_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9454364.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          310 a          136 c          154 g          298 t
ORIGIN      
        1 gaaaaaaaga aagtccattc tgtaaaatta aagttaccac aaccgaaatt ttataggagg
       61 actttctcat gcccaatttt actacagaaa ttatggaaac actaatcaat aaaggtgatt
      121 tagatgaatt atttcgatgt catttagaat tagctgttaa ttcattattg caggctgaat
      181 taacagcttt tcttgattat gaaaaatatg atcgtgctgg gttcaattca ggtaattccc
      241 gtaatgggaa ttattcacgt tcattcagaa cagagtatgg agaattaaat ttggtaattc
      301 ctagagatag aaatggtaag ttttctcaac aaactttacc tgcttataaa agaaccaatg
      361 actctttaga aaccactatt attcagctat ttcaaaaagg aattactatg tctgaaatct
      421 ctgaattgat tgaaaaaatg tacgggcatc actatacgcc acaaaccatt tcaaatatga
      481 ctaaaatagt ttctgaagat attattgcct ttaaagaaag gtccttagaa tccagatact
      541 ctgtcatttt tatggatgct actcacattc ctttaaaaag acaaactgtt tcaaaagaag
      601 cagtttatat tgtaattggc attcgattag atggaaccaa agaggttctt gggtttagtc
      661 ttgctccaac tgaatctgct tatgtttgga aagagattct tcaagaccta aaagaccgtg
      721 gtttaaaaga ggttttatta gtcgtaactg atggtttaag tggcattaac gatagtattc
      781 atagtgttta tccaaatgct caatttcaac aatgttgtgt ccatatatct agaaatatcg
      841 ctcataaggt tcgtgttagt gatcgacaag aaatctgtag cgatttcaaa ttagttta
//
LOCUS       DACDRK010000209          896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDRK010000209 DACDRK010000000
VERSION     DACDRK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.218"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>896
                     /locus_tag="IWJ77_002838"
     CDS             <1..>896
                     /locus_tag="IWJ77_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9454365.1"
                     /translation="SNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDG
                     TRFSQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGII
                     GAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSES
                     PKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQ
                     TNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNG
                     FSIFNYNFYAAGLNLKTARLLS"
BASE COUNT          347 a          109 c          155 g          285 t
ORIGIN      
        1 aagtaattta gaagatcttt tttatcaaaa tagaatgaca ctaaattctg agataaaaaa
       61 gttatttcta gagacagaag ttgacaaaat tgatattcaa aataaaaaga ttagttgtgt
      121 aaaaaaagat ggtacaagat tctctcaaga atatgacaaa gttatattag ctacaggttc
      181 aaagcaaatt atgctagata taccggggaa taatttagct aatatttttt cgataaagaa
      241 ttttccaaat gcaaaagaga tttttaaagc tttggatgat agtactatta aaaaagtagg
      301 aattattgga gcaggatata ttggagttga aattgctgaa gccataagaa aaagagggaa
      361 agaagtatat cttttcgatg ttgcagatag agtgttgtcg acttattacg accgtagttt
      421 ttcagataaa gttgaagaaa tactatcaaa aaatggaata catctttgtc tatcagaaag
      481 tcctaaaaaa tatttaggaa gaaaaaagat tgaaaaaata gtcactacaa gtggtaccca
      541 atactctatg gatatggtcg tacaagctat aggatttcta tctaatagtc caattgggga
      601 aaaggaactc ttgagagatg ttagtggagc atatctaact aatgaatacc agcaaacaaa
      661 tataaaagat atatatgcta ttggggattg tgctacaact ttttttacgt ccattaatag
      721 aagtagtgtt gatttttcta tttctaatgc tctaagaaca gcttatattg caactcaaca
      781 tattaacaat caagatttta caccttctgg ttcccaattt acaaatggct tttctatatt
      841 taattacaat ttttacgcag caggtttgaa tttaaaaaca gcacgattat taagtg
//
LOCUS       DACDRK010000210          867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDRK010000210 DACDRK010000000
VERSION     DACDRK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.219"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            62..>867
                     /locus_tag="IWJ77_002839"
     CDS             62..>867
                     /locus_tag="IWJ77_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9454366.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLN
                     RPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLG"
BASE COUNT          275 a          116 c          124 g          352 t
ORIGIN      
        1 aaaaatattt atagtttatg catttgttag tttattatat ttatttccag catttaaaaa
       61 catggaaata tttggctttt atgatacggc atttcacatt aatcgtgcgt taagtttaga
      121 atctattttt agctccccta ttaattttga aacgtttaga tcatatggca tgcaagtaaa
      181 taatttttac ccatggttaa ctctatatcc tttattttta ttgatcaagt ttactaattt
      241 agcaataggc tataaccttt ttctttatat tgttacgctc attactctat ttatatgtca
      301 ttatgtaatg tatgaaatta ccaaaaaaca tgttactagc tcctttttcg ctattatata
      361 tacaacatct tcttttagga gtgtagaaat atttcttcga ggggcaatgg gggaattact
      421 cgcaatgtca atacttcctc ttattttact cgggtttata aagctttacg actcaaaaaa
      481 agaatcttgg gttatgttgg cgattagcat gactttatta atttacactc atgttctttc
      541 tgtagctatg actatggtaa tgattataat tgctttaatt attcaatttt atcggaaaaa
      601 gaaaatagaa atcttcttaa ttattaaatt aataaaagct gctatagtta ctttacttct
      661 ttcactagcg tttataggtc ctatgattga gcagacattg tatcaagatc tgaatagacc
      721 ttatgtggat atattacaaa aaagagcaat ttatctgggt aaagagtttt tgattggaag
      781 tatagattca gaattactat catttggtat tggcttaact ttgcttattt gtttaattat
      841 actaacattt aactttaaaa aattagg
//
LOCUS       DACDRK010000211          856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDRK010000211 DACDRK010000000
VERSION     DACDRK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.220"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            83..>856
                     /locus_tag="IWJ77_002840"
     CDS             83..>856
                     /locus_tag="IWJ77_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9454367.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANA
                     NKHNFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
BASE COUNT          285 a          158 c          165 g          248 t
ORIGIN      
        1 ataaaatgcc agactgtaga caaagtccat tttatggatg atgtctacag tcttttcttt
       61 tataatagat ttatgaaaac gaatgcaagg agtgatagct atgatgtcta aaaacaatac
      121 gaatggtaga aatcaatttg cgatgcttac gattgatgat ctagttcctc aagatcatct
      181 cgttcgaaag attgatgccg cgcttgattt tgaatttatt tatccaatcg tcgaagccac
      241 ttactccgat ttaggtcgcc caagtatcga cccagtgatt ctgatcaaat tggtgtttat
      301 tcagtattta ttcggtatcc gttccatgcg acaaaccatt aaggaagtcg ataccaatgt
      361 cgcttaccgc tggtttttag gctactcctt cgaagagaaa atcccccatt tctcaacttt
      421 tggaaagaat tatgttcgcc gatttagaga aacgacggtg tttgaggata tttttgccta
      481 tattttagaa caagcagtta aagcaggttt tgtaacagag gataattttc atctagattc
      541 tacccacatc aaagctaacg ccaataaaca taattttacg aaagaaatga cccatgatga
      601 agcgaaagcc tatcaggatg aattagaaga tgagatcaat cgtcaacgaa tagaggctgg
      661 aaaacgccct tttactttgg acttagaaaa ggaagtgaag ctgaaagaaa gaaaaataag
      721 taaagctgat ccggaaagcg gctactacgt caaaggcgaa caagaaaaac aatttgctta
      781 ttctgcccat acttcatgcg acgacaatgg atttattctt agcacaatca tcactcctgg
      841 aaatattcat gacagt
//
LOCUS       DACDRK010000212          843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDRK010000212 DACDRK010000000
VERSION     DACDRK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.221"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            62..676
                     /locus_tag="IWJ77_002841"
     CDS             62..676
                     /locus_tag="IWJ77_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9454368.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT          298 a          150 c          156 g          239 t
ORIGIN      
        1 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
       61 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
      121 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
      181 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
      241 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta
      301 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
      361 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
      421 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
      481 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
      541 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
      601 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
      661 ggtcaatgac aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac
      721 atataattac tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa
      781 caaaccattt tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg
      841 tta
//
LOCUS       DACDRK010000213          829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDRK010000213 DACDRK010000000
VERSION     DACDRK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.222"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWJ77_002842"
     CDS             complement(138..428)
                     /locus_tag="IWJ77_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454369.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWJ77_002843"
     CDS             complement(546..806)
                     /locus_tag="IWJ77_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454370.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
BASE COUNT          348 a          130 c          122 g          229 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcata
//
LOCUS       DACDRK010000214          821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDRK010000214 DACDRK010000000
VERSION     DACDRK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.223"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..792
                     /locus_tag="IWJ77_002844"
     CDS             <1..792
                     /locus_tag="IWJ77_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454371.1"
                     /translation="KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGR
                     VINLVNRKSTTKFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNT
                     VVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWI
                     KELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRID
                     NIINYINSLSEPLKCRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT          341 a           83 c          142 g          255 t
ORIGIN      
        1 aagtccagtg aaagatttat gagcaatcaa ttagtgaaaa tcaaaaaatg gctccctatg
       61 attgatcaat tagtatctaa taataaggaa gctaaaaatt tatgtatttt tgtcttatta
      121 ttacatggaa gagttattaa cttagttaat cgaaaaagta cgactaaatt tgcaaactca
      181 ttctggtttt taaaaaatat taataaaaca gtggaaaaga atggagttta taaaggtgat
      241 tttcattttc aaaatgctga gactattgaa cgagaacatc attatttcga tttattagtg
      301 aatacagtgg tagaattagc gaaatgggca aagtcaaacg aaacttatag tcaagtactt
      361 aaaagaatta tgagtcaaga aacttcattt atctatgagt taagcaagtc aaatttgaaa
      421 tatacttata atgaagatga acttgaaatt ttagataatc cagaaaattc acaatggatt
      481 aaagagttga agagagaaga ctttattgag acactaagaa ttctaaaatc gttatttgat
      541 ttgaaattag ccaaagctac catcatggga attgaaagag attcatatta ttgggaggaa
      601 attggactat atgctcccca gattgaaggt gaagataaag agaaaaggat agacaacatt
      661 attaactata ttaatagttt aagtgaacct ttaaaatgta gaggacttga ttttatagga
      721 acattaggat ggacagaaac atactatgag aaaatatata aagaaataat tgaacaatat
      781 tttcctaaat aatttaataa ggattgattt atcgtaaaag a
//
LOCUS       DACDRK010000215          812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDRK010000215 DACDRK010000000
VERSION     DACDRK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.224"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>812
                     /locus_tag="IWJ77_002845"
     CDS             <1..>812
                     /locus_tag="IWJ77_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9454372.1"
                     /translation="LSLNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEH
                     RFYYLKELIDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADV
                     LKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKK
                     NLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLS
                     GGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVI"
BASE COUNT          316 a           94 c          145 g          257 t
ORIGIN      
        1 tttatcgcta aactctaaat taattatatt ggatgaaccg tcttcaaatt tggacttcaa
       61 agcgactaag aaacttgctg acttactttg tgatttaaaa aataaaggat ttactattgt
      121 tatagctgaa catcgttttt actatttgaa agaattaatt gatcaagttc tatttgttaa
      181 aaatagaact gtaaaaacta caactattca gttgttaaaa actgaaaaca ataacgcatt
      241 aaggaaattt gacattttta atagttcgat tgaagttccc tatatcgaaa aggtaagtgc
      301 agatgtatta aaagttgaag aaattactta tgaaaatatt ctaaaagaca taaattttga
      361 tgtcaaagtt ggagatgtta ttggtttggt agggaaaaac ggtgtgggga aaacaacctt
      421 actaagaata ctcatgaaga ttattcgccc taccaaagga aagataatcg aaaataaaaa
      481 aaatttgtca tcgccgtttt tagtaatgca agaaatggat taccaatttt tcacagaatc
      541 agtgaggagc gagttagccc tggggaatga aatagttagt aaagatcagc aagaaaaaat
      601 tttaaaaaaa atggaacttt ataaaatgaa agatcaatta ccgtttgatt tatccggtgg
      661 agaaaaacaa agattattgg tttctatagc ttcattgtca aaaactaatt tatttctatt
      721 tgatgaacct acaagtgggt tggattatat taatatggaa aggattgctg accttattaa
      781 agacttaaaa gaaaagggtg cagtagttat ag
//
LOCUS       DACDRK010000216          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDRK010000216 DACDRK010000000
VERSION     DACDRK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.225"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWJ77_002846"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWJ77_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9454373.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDRK010000217          764 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDRK010000217 DACDRK010000000
VERSION     DACDRK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.226"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..694
                     /locus_tag="IWJ77_002847"
     CDS             <1..694
                     /locus_tag="IWJ77_002847"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9454374.1"
                     /translation="SLCSSRFNSVIGVSNSVKEEFRYKQLIKDKFVLLPNTVNTERII
                     KMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVMIGEGPLFDEVK
                     MQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEAMLLETPVVAYN
                     VGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISAKKFSNMNRYQG
                     VLEKIYVSAALK"
BASE COUNT          292 a           77 c          146 g          249 t
ORIGIN      
        1 ttctttatgt tcgtctaggt ttaattcagt aattggtgtt tcgaattccg ttaaggaaga
       61 atttcgttac aaacaattaa taaaagataa atttgtgttg ttacctaaca ctgtaaatac
      121 tgaaagaatt ataaaaatgg ggaatgaatt tgttgaagat gatattgata ttttatatgt
      181 aggacgcatg gcagaaccta aaaatccaat aggtgcaatt aatgttttag aaaatgtagt
      241 aaaaaaatat tcaaaacatt tacgtgtcgt gatgattggt gaaggaccat tatttgatga
      301 agttaagatg caaatacaaa aaagaaactt aacagatgtt attgagttaa agggattcaa
      361 aagtaatccc tataagtaca tgaagaaatc taaagttcta ttaatgccat ctctttggga
      421 agggtttggc ctagtatcag tagaggcaat gttgttagag acgcctgttg tagcttataa
      481 cgtaggagga ttgaaggata ttatcgatga gacaaatgga tttctgtgtg gtagtgaagt
      541 tgaattatct aattcactaa tgaagctatt aaattcagaa gaacttagaa aaaaaatggg
      601 agaaaatgct gctattagtg caaagaaatt ttctaatatg aatcgttatc aaggagtatt
      661 agaaaaaatt tatgtatctg cagctttaaa ataaaatatg aattataaaa atagagatca
      721 attagtttta cgaaaaataa tacccattag tattttgttt caat
//
LOCUS       DACDRK010000218          763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDRK010000218 DACDRK010000000
VERSION     DACDRK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.227"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IWJ77_002848"
     CDS             99..293
                     /locus_tag="IWJ77_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454375.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..>763
                     /locus_tag="IWJ77_002849"
     CDS             562..>763
                     /locus_tag="IWJ77_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454376.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRI"
BASE COUNT          317 a           97 c          129 g          220 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatggaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tag
//
LOCUS       DACDRK010000219          733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDRK010000219 DACDRK010000000
VERSION     DACDRK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.228"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..>733
                     /locus_tag="IWJ77_002850"
     CDS             22..>733
                     /locus_tag="IWJ77_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9454377.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNV"
BASE COUNT          258 a          127 c          138 g          210 t
ORIGIN      
        1 aattataagg agtgagtaca aatgacctat acccatctta caacggatga acttgtaata
       61 atagagtctt atttcaaaat gaatcaatct gttgctaaaa ctgcccattg cttgaatcgt
      121 tcaagacaaa cgatccataa agtatatctg ttcttcaagc aaggaaaatc agccttagaa
      181 tattatcaac agtataagaa aaacaaatca aactgtggta gacgtccgct tgttttaccc
      241 gaggaacaat cagaatatat tcaaagaaag gttgttcaag gatggacacc cgatgtgatt
      301 gttggtcgtg cagcgtttcc tattagttgt tctgctcgta ccatttatcg tatgttcaaa
      361 aaggggctat ttgattcttc tgacttaccg atgaaaggca agcgtaaacc gaatggacat
      421 caagaaagac gtggaaaaca aactttccgc cgctctattc atgaacgtga aaaggattat
      481 agccaattct caaatgagtt tggtcacctt gaaggtgaca ctatcgtagg tctgaaacat
      541 aaaagtgctg taattacctt agttgaacga ttatcaaaag ttatcatcac attgaaaccg
      601 tgtggtagac aagcgattga tattgaaaaa aaattaaatc aatggtttga atctgtaccg
      661 aaaaacctat tcaaatccat cacttttgat tgtggaaagg aattttcaaa ttggaaacag
      721 atcagtaatg tca
//
LOCUS       DACDRK010000220          713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDRK010000220 DACDRK010000000
VERSION     DACDRK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.229"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..446)
                     /locus_tag="IWJ77_002851"
     CDS             complement(<1..446)
                     /locus_tag="IWJ77_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9454378.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          217 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cca
//
LOCUS       DACDRK010000221          698 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDRK010000221 DACDRK010000000
VERSION     DACDRK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.230"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(64..>698)
                     /locus_tag="IWJ77_002852"
     CDS             complement(64..>698)
                     /locus_tag="IWJ77_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9454379.1"
                     /translation="KKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTT
                     AFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYL
                     NFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIID
                     PSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          227 a          107 c           79 g          285 t
ORIGIN      
        1 attaaattta ttactcgaaa cgcatctcga cttaataaac tcttctctaa aacaggtact
       61 attctattca tctatatttc caactttcaa aatttcttta tcaattctat ttaatagttt
      121 atcagagtaa tccgatttaa cttttcttac aaataatttt tcactttctt ttatttgatc
      181 tatatccctt tcatcaaacc atttgttcat ggaaggatca ataatatgaa tatttgctaa
      241 tttcgaagtt tgtaaagtag tttcttcaat atttttcaat tcaagtggta aatctggtcc
      301 tccaataaca tttttatttt tgaacgagga attaaaaaaa agtgtatgaa aaaatttttc
      361 atctggggca aacataccta ttttaaaatt caggtagtct tgttgatttt gttcatacat
      421 ttgtattaat tctgttaggc attctttcga caaagcccac cattgagaac catgatacaa
      481 atcaaattta tggtgtctaa tttttggggg aagtttaatc atcctcagac aacgattaat
      541 tatagctcta aaaattttaa atgcagttgt gttattccta ttaattctag gataatcatg
      601 tttttgatat acatctatat gtttataata aaattcttta tgtttaattt gatctagatc
      661 tattccacga ataaactcca ctgaactatt ctttttaa
//
LOCUS       DACDRK010000222          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDRK010000222 DACDRK010000000
VERSION     DACDRK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.231"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(53..628)
                     /locus_tag="IWJ77_002853"
     CDS             complement(53..628)
                     /locus_tag="IWJ77_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9454380.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          124 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaac
//
LOCUS       DACDRK010000223          643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDRK010000223 DACDRK010000000
VERSION     DACDRK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.232"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..260
                     /locus_tag="IWJ77_002854"
     CDS             <1..260
                     /locus_tag="IWJ77_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454381.1"
                     /translation="LVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFI
                     LSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
     gene            262..>643
                     /locus_tag="IWJ77_002855"
     CDS             262..>643
                     /locus_tag="IWJ77_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454382.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTY"
BASE COUNT          210 a           72 c          101 g          260 t
ORIGIN      
        1 tacttgtatc aagtggtata attgctttta tatgtatcta tgctttctat gtgttaaata
       61 tctttagttt actcaaaaca ttttttagaa gtaaagtgaa ttatgcgttt cttggtattg
      121 ttttaatttt tatactatct atttttatag atgaaatggt ggaggctcat gtacttttca
      181 ataagcgcga tgtcatttca attgttttct ggtattattt gggaagcatt tcttacttgt
      241 atttaaaaaa ggagaattaa tatgaagaca gctttcattg cttggactcc tttacatttg
      301 attaatatat taaatgttta tcaaaatttt taccgagaat caaaaggaga tttatttatt
      361 tattctgaat ttgaaaacgc tagaagatat agtgagcgta taaaagaatt agggctattt
      421 aatgatgttg tttttgtgaa tcaccggaaa atcggttctt cctttaaacg taaaattaac
      481 ttgttaacta accataatca atttttctcg gatgatttat ccgtttatga tgaaattttt
      541 atacaggggg aaaattattt cacaaaaata cttttctcca atgtttacaa aaaaaatcca
      601 gatgtaaaac taaattatat agaagatggt ttgggtactt att
//
LOCUS       DACDRK010000224          637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDRK010000224 DACDRK010000000
VERSION     DACDRK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.233"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(107..511)
                     /locus_tag="IWJ77_002856"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IWJ77_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          202 a          121 c           99 g          215 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaa
//
LOCUS       DACDRK010000225          595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDRK010000225 DACDRK010000000
VERSION     DACDRK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.234"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            79..234
                     /locus_tag="IWJ77_002857"
                     /pseudo
     CDS             79..234
                     /locus_tag="IWJ77_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..>595
                     /locus_tag="IWJ77_002858"
     CDS             253..>595
                     /locus_tag="IWJ77_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9454383.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFK"
BASE COUNT          247 a           56 c          104 g          188 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taaga
//
LOCUS       DACDRK010000226          590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDRK010000226 DACDRK010000000
VERSION     DACDRK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.235"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            221..>590
                     /gene="spxA"
                     /locus_tag="IWJ77_002859"
     CDS             221..>590
                     /gene="spxA"
                     /locus_tag="IWJ77_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9454384.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLEL"
BASE COUNT          207 a           89 c          108 g          186 t
ORIGIN      
        1 tttgaattag atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt
       61 tactgtatat taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag
      121 ctatttaaaa gttgattatg tataatagaa acagatggga gattattatg atcttctgtt
      181 acaaaaaagt gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc
      241 cccaagttgt acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt
      301 tgttgaacgt aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca
      361 aatgacagaa gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact
      421 gaacatggat ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc
      481 tggattgctt cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga
      541 agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa ttagaattac
//
LOCUS       DACDRK010000227          573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDRK010000227 DACDRK010000000
VERSION     DACDRK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.236"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..514
                     /locus_tag="IWJ77_002860"
     CDS             <1..514
                     /locus_tag="IWJ77_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9454385.1"
                     /translation="ILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNL
                     ENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTIN
                     SLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKA
                     QQIVNEHTIQ"
BASE COUNT          276 a           73 c           81 g          143 t
ORIGIN      
        1 tattttaaat tcacaattgt cttcaaacaa ccaaactctt gaacaactaa gaactaatat
       61 tgaagtttcc aaacaaaaag tggcatcttt agaaaacgac aagaatcaat tagtatcaga
      121 gaaaactaat ttagaaaatc aattgtcagc aaaagactct gaattgcaag gaaaacaaaa
      181 tgaaattaat gcgaaaatag acgaaattaa ccaaaaagta gctgaaataa atcgattaaa
      241 tagcgattgg aataatcgtt tacaaaatga aaagaacaaa tttaatgaag aacaatctac
      301 tattaatagt ttaaaccagc aaattaatca aatgttgagt gaaaaaaata acattcaaaa
      361 tcaactgcaa gctactcagc aaaagctaga agaagaaaag tcaaaaaata gtagtttgaa
      421 taattacatt gaaaagcttc aaaaagctaa aaatgatgta gaggataccg caaataaagc
      481 acaacaaatt gtaaatgaac atacaatcca atagatatag ttttaattta acaaatggta
      541 tgtttggaga taatgagata gaatataaat gtc
//
LOCUS       DACDRK010000228          566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDRK010000228 DACDRK010000000
VERSION     DACDRK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.237"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          182 a          113 c           82 g          189 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatga
//
LOCUS       DACDRK010000229          558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDRK010000229 DACDRK010000000
VERSION     DACDRK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.238"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..374)
                     /locus_tag="IWJ77_002861"
     CDS             complement(<1..374)
                     /locus_tag="IWJ77_002861"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9454386.1"
                     /translation="MVIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRS
                     YTEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNIL
                     MRLFRAFLKPSETKRDENYVELA"
BASE COUNT          194 a          106 c           52 g          206 t
ORIGIN      
        1 gctaattcca cataattttc atcacgtttt gtttcgcttg gttttagaaa agctctaaaa
       61 agcctcataa ggatattggg tttctttata aaggctacac aactataaaa tccaaacggt
      121 attattcttt tctttatcat atacttatat aaaccactca tatctccaga atatgcaaga
      181 aatcctattg gcttatcatt ttgaatagca accaatatac cagaatcatt atattctgtg
      241 taagaacgat acatcaattc caaaaacttt tttcccatga aagtcaaaaa aaaatttgta
      301 aatgttctca tatgaatatc gacaatcgaa ttaattaact ctttatccga actttttatc
      361 tcttcaatca ccaaaactct tatcctccat tcattttatc tgattaataa actctcgttc
      421 accttctaag tcggcgtgat attctttacc aaatataaca aaaatagttt tacacattat
      481 tttcaaatca ttcatacaac taaattcact gatacttttc aaattgtaat acattttact
      541 gggaagaatt ttttctat
//
LOCUS       DACDRK010000230          543 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDRK010000230 DACDRK010000000
VERSION     DACDRK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.239"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          192 a           88 c           82 g          181 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttactct aggagtgagc atttttcgaa aaatactaaa aatttttcaa
      121 aataaaaaaa ggcatacaga caataaactg tctatacgcc tcacagactc tattctatac
      181 atctaatttt ggctgtcctg caaaaaatat gtgttagcct ccatcgaaag atcaaacttt
      241 ggaaaacatc attataactt tgcttggtaa gagctttgct aaaacatgat atgacttgta
      301 tacaaaatgt ggagtgtatt ccaataaata ggaaggatta caaaatttga aaccaaataa
      361 gtaaaaaata ctgtcacttt gaattaattt gaaatagtaa attctcatta aactattgaa
      421 atgctgatac caaaaaatga agtacttttc gttcatacca acttagagca cttcgttatc
      481 tatttatttc ttcatttggc ttatctctat aatctcatga tttggtccgt agaaattaaa
      541 gta
//
LOCUS       DACDRK010000231          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDRK010000231 DACDRK010000000
VERSION     DACDRK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.240"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWJ77_002862"
     tRNA            complement(5..90)
                     /locus_tag="IWJ77_002862"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWJ77_002863"
     tRNA            complement(107..178)
                     /locus_tag="IWJ77_002863"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWJ77_002864"
     tRNA            complement(190..262)
                     /locus_tag="IWJ77_002864"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWJ77_002865"
     tRNA            complement(280..361)
                     /locus_tag="IWJ77_002865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWJ77_002866"
     tRNA            complement(369..441)
                     /locus_tag="IWJ77_002866"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWJ77_002867"
     tRNA            complement(445..517)
                     /locus_tag="IWJ77_002867"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDRK010000232          515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDRK010000232 DACDRK010000000
VERSION     DACDRK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.241"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          173 a           76 c           86 g          180 t
ORIGIN      
        1 aaaaaaaagt tatctctatt cggttttttt gccctgacag cttccatggt tttgactgtt
       61 tatgagtatc cgacctttgc aacatcacaa ttacatttgg ttttctttct tctttttttc
      121 tatttacaca aaatatttta cactctcaac aaaagtatct tcgattaata cttgctcaag
      181 ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc tttttcgcag ctttttttgg
      241 acttatgaga agtacattcg ctggaagaat acgatataaa acgagctttt tcactaaaga
      301 tattaataga gtgaaagaaa caagcttttg ttgtcagttt tttcactttt ctaaaaatga
      361 aaataaaaaa ttctggaagt ggagaagaat tttgcttgct atactttgtc ttgtagaaaa
      421 gaggtagaca aaataatgca agctcaaata agcgattttc cataaccttg gtaggaatga
      481 caaattaaat aggaaaggga agtaataata tgaat
//
LOCUS       DACDRK010000233          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDRK010000233 DACDRK010000000
VERSION     DACDRK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.242"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..510
                     /locus_tag="IWJ77_002868"
                     /pseudo
     CDS             1..510
                     /locus_tag="IWJ77_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DACDRK010000234          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDRK010000234 DACDRK010000000
VERSION     DACDRK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.243"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACDRK010000235          500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDRK010000235 DACDRK010000000
VERSION     DACDRK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.244"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>500
                     /locus_tag="IWJ77_002869"
     CDS             <1..>500
                     /locus_tag="IWJ77_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9454387.1"
                     /translation="EYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAEI
                     LNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLLT
                     FHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTVI
                     QVPEVV"
BASE COUNT          217 a           68 c           70 g          145 t
ORIGIN      
        1 tgaatacgca atgaattatc taaatatcaa tactgagata atatcagatg aattaaccaa
       61 aaaatcgaaa gaatatgcaa atataaaaat cataaaagga tatgttcgtt ccatgttcga
      121 tattgcagaa atactaaatt atatcgaatt taatcgaact actaaaatta ttcaaagtat
      181 cactgcacct aaaaaaaatg ctttagaaga aaaaagaata caagaaggta agcaagcact
      241 atcatcaaaa gaacttacta attggataga agcagtaaat gatgatttta ataatcattt
      301 acttactttt catgattaca cactttttat gattaccctg tatcttggtg atagaaaaag
      361 tgagacttac gctttgcaat ggaaatatat tgattttgaa aaacaaacgg tccgtcttaa
      421 acatacttta gataaatatc aaagaaaaaa gtttacaaaa ggtcgaaaag ataccgtcat
      481 tcaagtacca gaagttgtaa
//
LOCUS       DACDRK010000236          493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDRK010000236 DACDRK010000000
VERSION     DACDRK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.246"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..171
                     /locus_tag="IWJ77_002870"
     CDS             <1..171
                     /locus_tag="IWJ77_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9454388.1"
                     /translation="NNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLY
                     HANEVLKIAEEK"
     gene            172..>493
                     /locus_tag="IWJ77_002871"
     CDS             172..>493
                     /locus_tag="IWJ77_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9454389.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDL"
BASE COUNT          196 a           55 c           97 g          145 t
ORIGIN      
        1 aataataaaa taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat
       61 atgtatgaga aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg
      121 aatgatttat atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt
      181 ttatttacta tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat
      241 ttggcaggaa agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa
      301 catcctgaac ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg
      361 acgactaagt taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat
      421 actgtaggga aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat
      481 atatttgatt taa
//
LOCUS       DACDRK010000237          491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDRK010000237 DACDRK010000000
VERSION     DACDRK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.247"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(108..>491)
                     /locus_tag="IWJ77_002872"
     CDS             complement(108..>491)
                     /locus_tag="IWJ77_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9454390.1"
                     /translation="AFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHK
                     GCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPV
                     GYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          142 a          106 c           96 g          147 t
ORIGIN      
        1 gacaaatttt ataaggtgca gaacttcttt ccgtatgcta ttccgattgt ccttgacaat
       61 gagcctcctc ggatgtgacg atcttcggca ggagctaaca gttaaagtta gactgcctcg
      121 ctagcgttag cacatccgag ctcattatca aggattcgct gcgccaatct ctggttacgg
      181 taaccaaccg gtgtctcgta gccaagtgta ccgtgcaacc gaaggtggtt ccaccaattg
      241 acatagtcaa ataactccaa atccaattgt tgtaaggttt caaatgtgta ttgatagaca
      301 aattctactt tcaacgactt ataagttgat tcagctacgg cattatcaaa aggacagcct
      361 ttatgactca atgatcgatt gatgtcaaaa gttgttaata attcatcaat agcttggtta
      421 tcaaactctt ttccacgatc agtatgaaaa atctcaacct ctgtcagagg ttgtttgata
      481 cggctaaatg c
//
LOCUS       DACDRK010000238          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDRK010000238 DACDRK010000000
VERSION     DACDRK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.248"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..259)
                     /locus_tag="IWJ77_002873"
     CDS             complement(62..259)
                     /locus_tag="IWJ77_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9454391.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DACDRK010000239          477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDRK010000239 DACDRK010000000
VERSION     DACDRK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.250"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..399)
                     /locus_tag="IWJ77_002874"
     CDS             complement(<1..399)
                     /locus_tag="IWJ77_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454392.1"
                     /translation="MIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLA
                     ELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLF
                     FLIFLLTYRKCQNYYVRILTALPFFLNLIIY"
BASE COUNT          197 a           63 c           62 g          155 t
ORIGIN      
        1 ataaataatc aggtttaaga aaaacggtaa tgcagtcaat atacgcacat aatagttttg
       61 acatttccta tatgttagaa ggaaaattaa gaaaaatagc aataaaaata agatattcat
      121 gtctaaaaaa aacggctttc caaatgatga aatacctaaa tctaacttat taagaaagtt
      181 gaaattttta tattcaggaa accatttatt aatttcatgt gcagcacgta atgcattccc
      241 tggagttgtc aaagagaaaa ttaattctgc taagcttatt attgaaaata ctgataactt
      301 ataattatat cgtccattga caatcaaata taaactaact atacttgtta gaacaaaaaa
      361 acaggcgttg acttgttctt gattagttaa aaatatcaat aaaggaatac ttacccaata
      421 tatttttcta tttatttctt ctcctcttaa gagttgaaaa aacggataaa aagttag
//
LOCUS       DACDRK010000240          464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDRK010000240 DACDRK010000000
VERSION     DACDRK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.251"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            165..>464
                     /locus_tag="IWJ77_002875"
     CDS             165..>464
                     /locus_tag="IWJ77_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9454393.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPT"
BASE COUNT          176 a           55 c           79 g          154 t
ORIGIN      
        1 catatttatt tataaatcaa aaatacagag aatagtatat aaatgtcctt tcctattctt
       61 aaaatgatgt ttgtacttac ttaaattagg tataattagt atatgttttg tgagaaattt
      121 agacttacaa taagcagtga atttataatc aacgtatagg agacatggat gaaatgaaac
      181 taattggtat agatttagac ggtactcttt tgaattctga ccagagcatt agtcaaaaaa
      241 atgcaagaac aatgaaacat ttaccggaag actgttttcc attcatttgt tctggaagag
      301 aagtagaaga cattaataat attctcaaaa aatctggttt aaggatacca gcagtagggt
      361 taaatggttc tgttggattt gatggagaaa aaaagatatt tgagtcttcc ttcgataaaa
      421 atgatgtaaa aaaaattgat tctgtcctat cgaaatttcc aacc
//
LOCUS       DACDRK010000241          432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDRK010000241 DACDRK010000000
VERSION     DACDRK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.252"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..403
                     /locus_tag="IWJ77_002876"
     CDS             <1..403
                     /locus_tag="IWJ77_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454394.1"
                     /translation="YFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFNHNTLFFFIMN
                     GLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALMPLTNPSTIQV
                     AGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT          108 a           83 c           60 g          181 t
ORIGIN      
        1 ctattttttt catcgacatc ctggtacgtt aaacgaagct tcaagcgtat tgattgcatt
       61 taaaacgttt tcttttttag caattctttt ttattcgttt aatcacaata cgcttttctt
      121 ctttatcatg aatggtttag tcgtgatttt atccattttt acttatttta ttttttcact
      181 catttttagc caattgaaat gtccgctctt ctttcgatta gttcctgtca cctgttttat
      241 ttacagcttg ttttcctttt ttgtccttgc tttaatgcca ctgaccaacc cttcgacgat
      301 ccaagtcgca ggaaatctgt tggatatgct cttcatcgtc agtctaggtg tatttatgtg
      361 gcaaagaaaa aacaaacaaa ataaaaagca ttttgaagcc tgactattac gacttcataa
      421 atgctttttt ag
//
LOCUS       DACDRK010000242          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDRK010000242 DACDRK010000000
VERSION     DACDRK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.253"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IWJ77_002877"
     CDS             complement(<1..339)
                     /locus_tag="IWJ77_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9454395.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACDRK010000243          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDRK010000243 DACDRK010000000
VERSION     DACDRK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.254"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IWJ77_002878"
     CDS             <1..379
                     /locus_tag="IWJ77_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9454396.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DACDRK010000244          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDRK010000244 DACDRK010000000
VERSION     DACDRK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.denovo.255"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>404
                     /locus_tag="IWJ77_002879"
     CDS             <1..>404
                     /locus_tag="IWJ77_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9454397.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DACDRK010000245         7935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.guided.095, whole genome shotgun sequence.
ACCESSION   DACDRK010000245 DACDRK010000000
VERSION     DACDRK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.guided.095"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1077
                     /locus_tag="IWJ77_002880"
     CDS             <1..1077
                     /locus_tag="IWJ77_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9454398.1"
                     /translation="SFELNQAVLNGWEDRKGRKINVPIADGGENTAEAIYYALRGTWR
                     TIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKPTDQTIRLASSYALGE
                     LIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRN
                     VLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWE
                     VDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQ
                     QTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAME
                     HRQTLEKTKILGQTLSRFFTILPK"
     gene            1335..2837
                     /locus_tag="IWJ77_002881"
     CDS             1335..2837
                     /locus_tag="IWJ77_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9454399.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2904..3431
                     /locus_tag="IWJ77_002882"
     CDS             2904..3431
                     /locus_tag="IWJ77_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9454400.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3642..4703
                     /locus_tag="IWJ77_002883"
     CDS             3642..4703
                     /locus_tag="IWJ77_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9454401.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4706..5602
                     /gene="murQ"
                     /locus_tag="IWJ77_002884"
     CDS             4706..5602
                     /gene="murQ"
                     /locus_tag="IWJ77_002884"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9454402.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5616..7598
                     /locus_tag="IWJ77_002885"
     CDS             5616..7598
                     /locus_tag="IWJ77_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9454403.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7663..>7935)
                     /locus_tag="IWJ77_002886"
     CDS             complement(7663..>7935)
                     /locus_tag="IWJ77_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9454404.1"
                     /translation="PTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         2641 a         1399 c         1787 g         2108 t
ORIGIN      
        1 tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc
       61 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcagg
      121 ggaacatggc gtaccattga tgggatggat ctcttattcc gtcagcgaaa aatcagctat
      181 ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg aatctgcaca agcattgggg
      241 ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag ttatgcctta
      301 ggagaactta ttaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga
      361 ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa
      421 gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac
      481 gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt
      541 caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagct
      601 ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat
      661 ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg
      721 ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag
      781 atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac
      841 ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
      901 atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc aattgtctta
      961 agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa
     1021 aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg
     1081 acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     1141 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     1201 ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt
     1261 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
     1321 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
     1381 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
     1441 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
     1501 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
     1561 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
     1621 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
     1681 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
     1741 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
     1801 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
     1861 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
     1921 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
     1981 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
     2041 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
     2101 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
     2161 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     2221 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     2281 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     2341 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     2401 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac cagccaaaac
     2461 atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     2521 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     2581 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     2641 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     2701 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     2761 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     2821 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     2881 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     2941 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     3001 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     3061 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     3121 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     3181 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     3241 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     3301 aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt
     3361 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     3421 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     3481 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga
     3541 aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga tatatactct
     3601 agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat
     3661 ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg
     3721 gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa
     3781 agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata
     3841 tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag
     3901 caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag attgccgact
     3961 ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg
     4021 aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat tattatccac
     4081 gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc
     4141 aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc
     4201 aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc
     4261 tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca gaaaacgttc
     4321 aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag
     4381 atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag
     4441 atgtcatacg aagcgtagat gcacgttttc gagagattcc gcctatccca gcaagaaata
     4501 cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg
     4561 gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta
     4621 aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat
     4681 ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc taacaacgga
     4741 acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact
     4801 aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga
     4861 accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg
     4921 agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg
     4981 tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc
     5041 tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt
     5101 gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg
     5161 tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca
     5221 agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt
     5281 gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga atatgatttc
     5341 tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc
     5401 aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga
     5461 ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt
     5521 catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg aagcaaacgg
     5581 atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg
     5641 aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg
     5701 tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg
     5761 aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca
     5821 tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat
     5881 taggagaaaa actttcttct gtaagtacag ctacagcaaa ttcatcagca tctggaaaag
     5941 accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat
     6001 ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat
     6061 ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg
     6121 gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg
     6181 ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa
     6241 caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag
     6301 caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcctag
     6361 gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac
     6421 ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc ggtctgattg
     6481 aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc ggtgggatca
     6541 actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca ttattcttgc
     6601 cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa atgattgatg
     6661 aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga caagtgggtg
     6721 cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa atgatcaaag
     6781 gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga gtaacattac
     6841 ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg gcagtgatcg
     6901 gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct ttgatcccat
     6961 tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct tatgctggtg
     7021 gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa gaagaattag
     7081 cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg
     7141 aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta gataaagcaa
     7201 gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg
     7261 gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg aaacacgcta
     7321 ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc aacacagttg
     7381 acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg acacctgata
     7441 cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa acatccatga
     7501 tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag
     7561 caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac aatagaaacg
     7621 aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat tgttttctgc
     7681 taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa
     7741 gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact gattattgaa
     7801 gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt catgttccag
     7861 atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac catttccgcc
     7921 gtctaaataa gtagg
//
LOCUS       DACDRK010000246         5985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.guided.107, whole genome shotgun sequence.
ACCESSION   DACDRK010000246 DACDRK010000000
VERSION     DACDRK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.guided.107"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(40..1026)
                     /locus_tag="IWJ77_002887"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="IWJ77_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="IWJ77_002888"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="IWJ77_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9454405.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="IWJ77_002889"
     CDS             1851..3005
                     /locus_tag="IWJ77_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9454406.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="IWJ77_002890"
     CDS             3220..4188
                     /locus_tag="IWJ77_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9454407.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="IWJ77_002891"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="IWJ77_002891"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9454408.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5826
                     /locus_tag="IWJ77_002892"
     CDS             5218..5826
                     /locus_tag="IWJ77_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9454409.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1161 c         1347 g         1666 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
     5581 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
     5641 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
     5701 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
     5761 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
     5821 aaataaactt ttaaccgttg cacggacaaa ctatataagc taactctttc ggcaggaaac
     5881 ccgacgtatg taactggttc ttagggaatt tatatatagt agatagtatt gaagatgtaa
     5941 ggcagagggt tctgttgcaa agttttaaat aaagaataaa atccc
//
LOCUS       DACDRK010000247         5637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.guided.114, whole genome shotgun sequence.
ACCESSION   DACDRK010000247 DACDRK010000000
VERSION     DACDRK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.guided.114"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1991
                     /locus_tag="IWJ77_002893"
     CDS             <1..1991
                     /locus_tag="IWJ77_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9454410.1"
                     /translation="APLIQIFLGVVLGLTEWGQSIDFEPELFLVMIIAPLLFREGEKA
                     DISSILKNFGTILFLAFGGVILTLVGVGATLSFLLPSVPLAACFAFGAALGPTDAVAV
                     SSLSGRVNIPKKAMHILEGEGLLNDASGVTAFQFALGALITGSFSAVNAGMSLVVSSI
                     GGALIGFLLVWFKQKIIHLIEKASAQDVTGYLLIELLLPFLAYVLAEAFDVSGIIAAV
                     AAGILQASGFRKISVFDAELSSLSHSTWTTIAFTLNALVFIFLGIELTQVFSPVWGDG
                     LYPNGLLLAIIVLISVMLFVIRFISISLFYVFKDGSKKFKKQLNEILILTFGGVKGTV
                     SLATIFILPFSINNMMFYQRSLLLFLTAGVILVTLVIGIIVLPMLTETEEAKSTDLNA
                     LMILEEVVEVLRKEIKEIDRNTKEFLATEAVIENYQERIRDLYLEDLTDDEKQEVQEI
                     QALILSIERDGLDESYRSGKLSSNGYRFYSRFLSRFEHSITSQILSFIGFWFIVVRRL
                     MRIVLHPKMFWQRRYADRQTFISDEDIQEIRETYQKNTQLIIESLDNLTDIYDETMVQ
                     FFIQQRKLEGIKMNSGNLISSWMIQQDSLFTKKMLRGYYLERKIIDEYEVAEKITTFS
                     ANSYRRNINLLESYTMNKPSDNFSFRFAFRAKSKKRVQ"
     gene            2156..4042
                     /locus_tag="IWJ77_002894"
     CDS             2156..4042
                     /locus_tag="IWJ77_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9454411.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4054..5001
                     /locus_tag="IWJ77_002895"
     CDS             4054..5001
                     /locus_tag="IWJ77_002895"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9454412.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5021..5548
                     /locus_tag="IWJ77_002896"
     CDS             5021..5548
                     /locus_tag="IWJ77_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9454413.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
BASE COUNT         1850 a          937 c         1239 g         1611 t
ORIGIN      
        1 cagctccact gatccaaatt ttccttggag tggtattagg tctgacagag tggggacaat
       61 caatcgattt tgaacctgaa ttgttcttag ttatgattat tgcaccattg ttatttagag
      121 aaggagaaaa agcagatatt tcttcgattc tcaaaaattt cgggacaatt ttgttcttgg
      181 catttggagg agtcattttg acccttgtcg gggtaggtgc gacgttatca ttcctgttgc
      241 caagtgtccc acttgctgcc tgctttgctt ttggagcagc gttaggtccg acagatgcgg
      301 tagcagtcag ttctctttct ggacgagtga atatccctaa aaaggcgatg catattttgg
      361 aaggagaagg tcttttgaat gatgcatctg gtgttactgc cttccagttt gctcttggtg
      421 cgctgatcac cggatctttt tcagcagtaa acgcggggat gagtctagta gtttctagta
      481 ttgggggagc tttgatcggc tttttgcttg tctggttcaa acaaaaaata attcatttga
      541 tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct gatcgaactt ttgcttcctt
      601 ttctagccta tgtattggca gaggcatttg atgtatctgg aatcattgct gcagtggcag
      661 caggtattct ccaagcttct ggttttagga aaatctctgt gtttgatgca gaattgtcga
      721 gcttgtccca cagtacatgg acaactattg cttttacact gaatgcatta gtatttatat
      781 ttttaggaat cgaattgacg caagtgttct cgccagtatg gggagacggt ttatatccaa
      841 acggtctttt attagcgatt atcgtattaa tctctgtcat gctttttgtg atcagattca
      901 tttctatcag tcttttttat gtattcaaag atggcagtaa aaagttcaaa aaacagttga
      961 acgagatctt gatcttaaca tttggcggtg taaagggaac agtcagtttg gctacgattt
     1021 ttattttgcc attttcaatc aataatatga tgttttacca gcggtctttg cttttgtttc
     1081 tgacggcggg tgtcatctta gtgactttgg tcatagggat cattgtcttg cctatgctga
     1141 cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt gatgatttta gaagaggtcg
     1201 ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa tacaaaagaa tttttagcca
     1261 ctgaagcagt gatagaaaat tatcaagaac ggatacgtga tctgtacttg gaagatttaa
     1321 ccgatgatga aaagcaagaa gtacaagaaa tacaagcact gattttgtcg atcgaacggg
     1381 atggattgga tgaaagttat cgttcgggca agctttcttc taatggttat cgtttttact
     1441 cccgttttct ttctcgattc gaacattcta tcacaagcca gattttgtct tttatcggct
     1501 tttggttcat tgttgtccgt aggttaatgc gaatcgttct ccatccgaaa atgttttggc
     1561 aaagaagata tgctgatcgt cagacattta ttagtgatga agatatccaa gaaatcagag
     1621 agacatatca aaaaaatact cagttgatta ttgaaagttt agataacttg actgatattt
     1681 atgatgaaac gatggttcaa ttttttatcc agcaacgcaa acttgaagga ataaaaatga
     1741 actcggggaa tttgatttct tcgtggatga tccagcaaga tagtctgttt acgaaaaaaa
     1801 tgctaagagg ttattatctt gaaagaaaaa tcattgatga atacgaagtt gctgaaaaaa
     1861 tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa tttgttagaa tcttatacga
     1921 tgaacaaacc atctgataac ttttctttcc gatttgcgtt tcgagcaaag tcaaaaaaac
     1981 gagtccaata aaataaaaag gtaaaaattt tcaagtttta taaaaaacaa aacatatcag
     2041 aacaggatcg aatcggtcct gttctttttt tgagaacaca aaaagaacaa ataccaaaaa
     2101 aattttcctg aagttcacta tttttatgat acaatatcca agataaggag ggaacatgtt
     2161 gaaagaatta aaagtaactg acctcaaaaa aacttatggt gaaaaagatt tgtttgacca
     2221 gatttctttt ttggtccacg aaaaagaccg tatcgggttg attgggacga acgggacggg
     2281 taaaacaagt ctcttaaata ttattgccgg tatcgatagc agcgacggtg atcggcaaac
     2341 agtgttttat ccaactgatt accgtattgg ttatctttct caagcagctg aattttccga
     2401 tgaattaact gttctccaag cagtctttca aggaaacagc cccttgatcc aaactgttcg
     2461 agcatatgag gaagcattga tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa
     2521 acgttatgct aaagcagaag aacaaatgaa taaagaagat gcttggacta cagataccaa
     2581 cgcaaagatt attttgcaaa aattaggaat cagtgaatta gataaaaaaa tcagtacttt
     2641 atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg ttgattgatg aacccgattt
     2701 actattacta gacgaaccaa caaaccattt agattatgaa gcgatcgagt ggttggaaaa
     2761 ctatttgaaa caataccgtg gcgcactatt gatggttacc cacgatcgtt attttctaga
     2821 tagagtagca aaccgaatct tcgaactttc atttggaaaa ttatacgagt ataaaggaaa
     2881 ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga
     2941 aaagcgcaag cggctttata aacaggaatt agcttggatg agggctggcg tacaggcacg
     3001 tggcaccaag cagcaagcac ggatcaaccg tttcgaagat ctaaaggaga acttgtacca
     3061 agtaaaccaa gaagatgatt tagagctgaa cctagcgaca caacgtttag ggaaaaaagt
     3121 actggaaatc aaagatggca gttaccgtat aaatgaccaa acactgctag aacatttaga
     3181 tttattgatt caatctcggg aaagactagg tattactggt aaaaatggtg cgggaaaatc
     3241 gacattgctt aatattctag ctggcagaat tcccctggat agcggtacaa tgtctatcgg
     3301 tgaaacggtt catcttgcgt attatacaca agaaaatgaa gaaatggcac cagataaacg
     3361 gatgattgct tacttacaag aagctgctga agaagcaaaa acagcagatg gcagtcaaat
     3421 cggagtagca gaattacttg aacgattttt atttccaaga tttatgcatg ggacactgat
     3481 aggcaaactt tcaggcggag aaaaacgccg gttatttttg ttgaaactgc tgattcagca
     3541 acctaatgtt ttattattgg atgaaccgac caatgatttg gacatagcaa cattgacgat
     3601 tttggaagat tattttagaa gttttccagg tgctgtgatc actgtttccc atgaccgtta
     3661 tttcttggac aaggtagcag ataagttatt agtattccaa ggaaacggaa aacaagaact
     3721 ttattacgga aacatgtcca gttatttact gaagcaaaaa gagacccagc agcctgctga
     3781 aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa aagaagaaat tatcttacat
     3841 ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca gaacttgaag aaaaaatctc
     3901 attgctccaa gaagagatga atcatcaagg agataatttc actcgattac aagaattgca
     3961 aaacgacgta tctgaaacag aagcacaact tgaagaaaaa atggcacggt gggaatactt
     4021 gagtgaatgg gtagaagatt aggaggaaaa aggatggaac aagcgtatct tgacttagga
     4081 agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa caggcacagg cacattaagc
     4141 ttattcggat accaaatgcg gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa
     4201 cgagtacctt ttggattgat caaaagcgaa ttgctttggt ttttaaaagg agatacgaat
     4261 atccgctatc tattacaaaa taaaaaccat atttgggatg aatgggcatt tgaacgctac
     4321 atcaaaagtc cagactataa tggaccagat atgactgatt ttggccatag atgtttgaca
     4381 gatcctgaat tcaatgaaga atataaaaag cagagcaagc ttttttgcga gaagatccta
     4441 accgatgatt cctttgctga aacatacggc gatcttggac acatctacgg ctatcaatgg
     4501 cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca
     4561 atcaaaaaga ctcctgattc tagaagattg atcgtttctg catggaatcc ggaagatgtt
     4621 cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat tttatgttca agagggacgg
     4681 ctaagctgcc aattgtatca acgcagtggg gatgtgtttc taggcgttcc gttcaacatt
     4741 gcaagttatg cgttgctgac tcacctaatt gcacatgaaa caggtttagc ggttggcgaa
     4801 tttgttcaca cattaggaga tgctcatctt tatatcaatc atatcgaaca aatgaaagaa
     4861 caattgagcc gagaaatacg cgcatttccg actttagttt tgaatcagga aaagcagtca
     4921 gtatttgatt ttgagatgga agatatccaa ttagaaggat atgacccaca tccggcgatc
     4981 aaagctccta tcgctgttta gaatcagaaa ggatgagaaa atgttagctg caatttgggc
     5041 acaggatgaa aacggattga ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga
     5101 tctgaaattc ttcaaacaaa tgacagaagc aaatacacta gtgatgggac gaaaaacatt
     5161 cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg attgttttga cgagagattc
     5221 ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga
     5281 aaaagaaaca gacggtatct ttttcatcgc tggagggtca gctgtttatc aagaatttct
     5341 gccattttgt acgatattat accgtacagt catccatcat gtattcgatg gagatgctta
     5401 ttttccacct gttgactggt ctgattggtc actgatcaat actagtcaag gagaaatgga
     5461 tgaaaaaaat cgttatccgc atcaatttga aacgtatcag cgtaatgaga atcctgtaaa
     5521 aagcgggaca ggcgaattga cgatataaaa aagctatgga tagactagct gttatttaga
     5581 gctgtcccat ccatagcttt atttttttta ttaagtaaag aataaaacag agaagta
//
LOCUS       DACDRK010000248         2883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.guided.146, whole genome shotgun sequence.
ACCESSION   DACDRK010000248 DACDRK010000000
VERSION     DACDRK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.guided.146"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(3..500)
                     /gene="dfrG"
                     /locus_tag="IWJ77_002897"
     CDS             complement(3..500)
                     /gene="dfrG"
                     /locus_tag="IWJ77_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9454414.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            862..1902
                     /locus_tag="IWJ77_002898"
     CDS             862..1902
                     /locus_tag="IWJ77_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9454415.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            2000..2188
                     /locus_tag="IWJ77_002899"
     CDS             2000..2188
                     /locus_tag="IWJ77_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9454416.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2246..2599)
                     /locus_tag="IWJ77_002900"
     CDS             complement(2246..2599)
                     /locus_tag="IWJ77_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9454417.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT          946 a          486 c          457 g          994 t
ORIGIN      
        1 cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata cggattttta
       61 tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt cacttctgga
      121 aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt ctcaacataa
      181 gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc ttcgttttta
      241 cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa ggtaaacccc
      301 ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat tgattcaagg
      361 ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata ttcccagtcc
      421 ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt atccatcgca
      481 gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc atctaatttc
      541 ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata agcaattttt
      601 tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa ttatcatttt
      661 caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa atgaaaaaag
      721 tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta cttacttgca
      781 tatatacgcc tttatagtgg agtactacat ttacgagatt cggttagagt atcagaaaaa
      841 gaaaaaataa aagttacaga aatgtatact tcaataaatg gtgaattatg taagattgat
      901 agagcttatt ctggagaaat tgttattttg caaaatgagt ttttgaagtt aaatagtgtt
      961 cttggagata caaaactatt gccacagaga aaaaagattg aaaatccgca ccctctacta
     1021 caaacaactg ttgaaccgag taaacctgaa cagagagaaa tgttgcttga tgcccttttg
     1081 gaaatctcag atagtgatcc gcttctacga tattacgtgg attctacgac acatgaaatt
     1141 atactttctt tcttagggaa agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag
     1201 tatcatgtgg agatagaact aaaagagcct acagtcattt atatggagag accgttaaaa
     1261 aatgcagaat ataccattca catcgaagtg ccgccaaatc ctttctgggc ttccattggt
     1321 ttatctgtat caccgcttcc gttgggaagt ggaatgcagt atgagagctc ggtttctctt
     1381 ggatacttaa atcaatcatt tcaaaatgca gttatggaag ggatacgcta tggttgcgaa
     1441 caaggattat atggttggaa tgtgacggat tgtaaaatct gttttaagta cggtttatac
     1501 tatagccctg ttagtactcc agcagatttt cggatgctta ctcctattgt actggagcaa
     1561 gcctttagaa aagctggaac agaattgtta gagccatatc ttagttttaa agtttatgca
     1621 ccacaggaat atctttcacg ggcatataac gatgctccca aatattgtgc aaatatcgta
     1681 aatactcaac tgaaaaataa tgaggtcatt attattggag aaattcctgc tcgatgtatt
     1741 caagattatc gcaatgattt aacttttttt acaaatgggc ttagtgtttg tttagcagag
     1801 ctaaaaggat atcaggttac cactggcgaa cctgtttgcc agacccgtcg tctaaatagt
     1861 cggatagata aagtaagata tatgttcaat aaaataactt agtgcgtttt atgttgttat
     1921 ataaatatgg tttcttatta aataagatga aatattcttt aatatagatt tgaattaaag
     1981 tggaaaggag gagattgtta ttataaacta caagtggata ttgtgtccta tttgtggaaa
     2041 taaaacaaga ctacgaatac gagtggatac tatacttaaa aatttccctt tatactgccc
     2101 caaatgtaag aacgaaactt taattaatgt tcaaaaaatg aatataataa caatcaaaga
     2161 gccagacgcc aagacgcaga gccgataatt tgagaaatga aactctcatc ttatcggctc
     2221 tttttgttta tctgaatttt actgactagc cttcaatatt tcttgcttca ttgatacctt
     2281 ttgctagtgc ttccattaag gatagttctt tgtctgtaaa gctatccatg tatttttcta
     2341 cctgtaacct tcgagtgctt ttaatcaaat tatctgtagg taagaaaaat tcatcaatag
     2401 atacatggag taatgataca agatcataca gcacttgtgt acttgggtgt tgtcctttgt
     2461 tttctatatt tgttaaataa cgtgggtcaa tttcaatcat tgttcccact tgttcacgag
     2521 tgagaccccg tttcattcga gcctctttaa tcgctaaacc aacggctcta aaatcgtatt
     2581 tatcttcttt tttacgcata ctaaatcacc tctatacatt ttactgttcc tatataacca
     2641 taaacaggta tagaaaaaca tgttatatag tttatgagtt cctatagaac actgtggaat
     2701 ttattaatgc gatgaatcca atttcaaata gaaaggtcgc tgattttatt tacctataca
     2761 actttataaa atttagtcat actcctagaa agtagataaa attggcgact taatggaggg
     2821 aaaagtcgcc atgagaaaga taatctgtca tctagttctc ttaattaagt tcttatcaaa
     2881 aaa
//
LOCUS       DACDRK010000249         2681 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.guided.151, whole genome shotgun sequence.
ACCESSION   DACDRK010000249 DACDRK010000000
VERSION     DACDRK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.guided.151"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..212
                     /locus_tag="IWJ77_002901"
     CDS             <1..212
                     /locus_tag="IWJ77_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9454418.1"
                     /translation="REVYVALTEKAQKKIKHCEMDHKDTGSVLSPAFSDVEMKQFLTL
                     LDKLNTQLCNITTETKEVNNNGTKK"
     gene            196..825
                     /locus_tag="IWJ77_002902"
     CDS             196..825
                     /locus_tag="IWJ77_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9454419.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1179..2657
                     /gene="msr"
                     /locus_tag="IWJ77_002903"
     CDS             1179..2657
                     /gene="msr"
                     /locus_tag="IWJ77_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9454420.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT          943 a          447 c          566 g          725 t
ORIGIN      
        1 accgggaagt gtatgtagcg ctaacagaaa aagcccagaa gaaaatcaag cattgtgaaa
       61 tggaccataa agatacaggt tccgtgctat ccccggcttt cagcgatgta gaaatgaaac
      121 aatttctaac acttttagat aaattgaata cccaactttg caatataaca acagaaacga
      181 aagaggttaa taataatgga acaaaaaaat gatttttcaa caattatgac tggtagaaga
      241 tccgttcgcg tatatgatga aacatacaaa atcccacacg aagaaatgct ggaaatgatc
      301 aaagaagcga cgactgcacc ttcttctgtc aatatgaaac catggcgctt tgtggtcgct
      361 gaaagcgagg aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac gagacaaaat
      421 gatacttctt ctgcaatggt catgattttt ggcgatatgc aatgttacga atatggcgaa
      481 gaaatctaca accaagctta cgaatcaggg aagatgccaa aagaagtcag agatcaacaa
      541 ttagctgcga tcattcctta ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc
      601 aaagtcgatt ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat
      661 gaaacaaatc caattggtgg attcgaagca gatcagttgg cagaggcatt tggtttggat
      721 aaagaccgtt atgttccagt tattatttta tcaataggaa aagcggtaga agaaggttac
      781 gagtctgttc gtttagcacc agaaaagatc actatgttcg aataaaaggt tttcagttac
      841 tattcagtag ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg
      901 gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt
      961 caaaagacat gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca
     1021 gtagaatagg tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag
     1081 taggaaaaga tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg
     1141 ataattgttg ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata
     1201 taacaaatct acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg
     1261 tctatcagca agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc
     1321 tcaaactaat agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct
     1381 tcaactacct gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag
     1441 ccagtcattt ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag
     1501 aacgaaagat cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg
     1561 agccaacaac ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt
     1621 attatggtac gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga
     1681 aaatctggga agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc
     1741 tcactcaaaa ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag
     1801 agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa
     1861 aagtgtccag taaaaagaaa aagcaacaaa tcagactgga tcggttgtct tcctctaaac
     1921 aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc
     1981 aaaaaatagg agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa
     2041 aatctcttga actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa
     2101 gtgggagaac attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga
     2161 ttgtcggtga aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag
     2221 aaggaatcct tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa
     2281 tgacatctga agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc
     2341 ttgtccgtag tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat
     2401 gtacattaag tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa
     2461 gtaatgtgtt gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt
     2521 tagagaagct aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact
     2581 ttgtcgagcg tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac
     2641 ttacgagaaa tttttaaatg tgctacgatg attttcgtga c
//
LOCUS       DACDRK010000250         2535 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.guided.155, whole genome shotgun sequence.
ACCESSION   DACDRK010000250 DACDRK010000000
VERSION     DACDRK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.guided.155"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(266..1369)
                     /locus_tag="IWJ77_002904"
     CDS             complement(266..1369)
                     /locus_tag="IWJ77_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9454421.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1922..2470
                     /gene="aac(6')"
                     /locus_tag="IWJ77_002905"
     CDS             1922..2470
                     /gene="aac(6')"
                     /locus_tag="IWJ77_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9454422.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          820 a          446 c          490 g          779 t
ORIGIN      
        1 acatggtaca gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac
       61 agttgccaaa taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct
      121 taattaagga actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc
      181 tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct
      241 gcttgatggg ggttttagca attatttaac tgattttctt atatccttgc tggctttctt
      301 gtgtagcatg ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt
      361 cataaggcac atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat
      421 cggtcgtgac attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa
      481 acacatagat tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac
      541 cataactgac agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt
      601 aatagaatga gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc
      661 cggtagtaac aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg
      721 cactatcttt caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga
      781 tttggattcc taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt
      841 cgacttcttt ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg
      901 caataattgc tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt
      961 ccattacgcc ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac
     1021 caatagtggg aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat
     1081 cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct
     1141 ggatcaactg ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa
     1201 cctctatttc ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct
     1261 tccctccaat cagcactact tttgtcccat attctttgac gattccattt gctttttcat
     1321 acgcatctgt tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct
     1381 tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag
     1441 atggagtaga ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg
     1501 aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat
     1561 gaatttttgt tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa
     1621 aattctaaaa attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt
     1681 tttttttaaa gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat
     1741 tgatggcttt cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt
     1801 tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag
     1861 cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg
     1921 tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt
     1981 actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat
     2041 gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat
     2101 tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag
     2161 ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc
     2221 ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt
     2281 aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg
     2341 tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa
     2401 tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa
     2461 ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat
     2521 ttacgaaaat tactt
//
LOCUS       DACDRK010000251         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.guided.176, whole genome shotgun sequence.
ACCESSION   DACDRK010000251 DACDRK010000000
VERSION     DACDRK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.guided.176"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWJ77_002906"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWJ77_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWJ77_002907"
     CDS             394..477
                     /locus_tag="IWJ77_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9454423.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWJ77_002908"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWJ77_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9454424.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDRK010000252         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.guided.186, whole genome shotgun sequence.
ACCESSION   DACDRK010000252 DACDRK010000000
VERSION     DACDRK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.guided.186"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWJ77_002909"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWJ77_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWJ77_002910"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWJ77_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9454425.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDRK010000253         1196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20418_2015
            SAMN12639623-rid9883103.guided.199, whole genome shotgun sequence.
ACCESSION   DACDRK010000253 DACDRK010000000
VERSION     DACDRK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639623
            Sequence Read Archive: SRR10030782
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742087.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742087.1
            Genome Coverage       :: 52x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:45:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,911
            CDSs (total)                      :: 2,846
            Genes (coding)                    :: 2,772
            CDSs (with protein)               :: 2,772
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 27 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 21 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639623-rid9883103.guided.199"
                     /isolate="E20418_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            259..>1196
                     /gene="vanY"
                     /locus_tag="IWJ77_002911"
     CDS             259..>1196
                     /gene="vanY"
                     /locus_tag="IWJ77_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9454426.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          428 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaataa
//
